---
OA_place: publisher
OA_type: hybrid
_id: '20807'
abstract:
- lang: eng
  text: Human glutamate carboxypeptidase 2 (GCP2) from the M28B metalloprotease group
    is an important target for therapy in neurological disorders and an established
    tumor marker. However, its physiological functions remain unclear. To better understand
    general roles, we used the model organism Caenorhabditis elegans to genetically
    manipulate its three existing orthologous genes and evaluate the impact on worm
    physiology. The results of gene knockout studies showed that C. elegans GCP2 orthologs
    affect the pharyngeal physiology, reproduction, and structural integrity of the
    organism. Promoter-driven GFP expression revealed distinct localization for each
    of the three gene paralogs, with gcp-2.1 being most abundant in muscles, intestine,
    and pharyngeal interneurons, gcp-2.2 restricted to the phasmid neurons, and gcp-2.3
    located in the excretory cell. The present study provides new insight into the
    unique phenotypic effects of GCP2 gene knockouts in C. elegans, and the specific
    tissue localizations. We believe that elucidation of particular roles in a non-mammalian
    organism can help to explain important questions linked to physiology of this
    protease group and in extension to human GCP2 involvement in pathophysiological
    processes.
article_number: BSR20230502
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Lucie
  full_name: Panska, Lucie
  last_name: Panska
- first_name: Stepanka
  full_name: Nedvedova, Stepanka
  last_name: Nedvedova
- first_name: Vojtech
  full_name: Vacek, Vojtech
  last_name: Vacek
- first_name: Daniela
  full_name: Krivska, Daniela
  last_name: Krivska
- first_name: Lukas
  full_name: Konecny, Lukas
  last_name: Konecny
- first_name: Filip
  full_name: Knop, Filip
  id: 25f3131f-6e7c-11ef-8296-b64ccd4a1b69
  last_name: Knop
  orcid: 0000-0002-3845-3465
- first_name: Zsofia
  full_name: Kutil, Zsofia
  last_name: Kutil
- first_name: Lubica
  full_name: Skultetyova, Lubica
  last_name: Skultetyova
- first_name: Adrian
  full_name: Leontovyc, Adrian
  last_name: Leontovyc
- first_name: Lenka
  full_name: Ulrychova, Lenka
  last_name: Ulrychova
- first_name: Judy
  full_name: Sakanari, Judy
  last_name: Sakanari
- first_name: Masako
  full_name: Asahina, Masako
  last_name: Asahina
- first_name: Cyril
  full_name: Barinka, Cyril
  last_name: Barinka
- first_name: Marie
  full_name: Macurkova, Marie
  last_name: Macurkova
- first_name: Jan
  full_name: Dvorak, Jan
  last_name: Dvorak
citation:
  ama: Panska L, Nedvedova S, Vacek V, et al. Uncovering the essential roles of glutamate
    carboxypeptidase 2 orthologs in Caenorhabditis elegans. <i>Bioscience Reports</i>.
    2024;44(1). doi:<a href="https://doi.org/10.1042/bsr20230502">10.1042/bsr20230502</a>
  apa: Panska, L., Nedvedova, S., Vacek, V., Krivska, D., Konecny, L., Knop, F., …
    Dvorak, J. (2024). Uncovering the essential roles of glutamate carboxypeptidase
    2 orthologs in Caenorhabditis elegans. <i>Bioscience Reports</i>. Portland Press.
    <a href="https://doi.org/10.1042/bsr20230502">https://doi.org/10.1042/bsr20230502</a>
  chicago: Panska, Lucie, Stepanka Nedvedova, Vojtech Vacek, Daniela Krivska, Lukas
    Konecny, Filip Knop, Zsofia Kutil, et al. “Uncovering the Essential Roles of Glutamate
    Carboxypeptidase 2 Orthologs in Caenorhabditis Elegans.” <i>Bioscience Reports</i>.
    Portland Press, 2024. <a href="https://doi.org/10.1042/bsr20230502">https://doi.org/10.1042/bsr20230502</a>.
  ieee: L. Panska <i>et al.</i>, “Uncovering the essential roles of glutamate carboxypeptidase
    2 orthologs in Caenorhabditis elegans,” <i>Bioscience Reports</i>, vol. 44, no.
    1. Portland Press, 2024.
  ista: Panska L, Nedvedova S, Vacek V, Krivska D, Konecny L, Knop F, Kutil Z, Skultetyova
    L, Leontovyc A, Ulrychova L, Sakanari J, Asahina M, Barinka C, Macurkova M, Dvorak
    J. 2024. Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs
    in Caenorhabditis elegans. Bioscience Reports. 44(1), BSR20230502.
  mla: Panska, Lucie, et al. “Uncovering the Essential Roles of Glutamate Carboxypeptidase
    2 Orthologs in Caenorhabditis Elegans.” <i>Bioscience Reports</i>, vol. 44, no.
    1, BSR20230502, Portland Press, 2024, doi:<a href="https://doi.org/10.1042/bsr20230502">10.1042/bsr20230502</a>.
  short: L. Panska, S. Nedvedova, V. Vacek, D. Krivska, L. Konecny, F. Knop, Z. Kutil,
    L. Skultetyova, A. Leontovyc, L. Ulrychova, J. Sakanari, M. Asahina, C. Barinka,
    M. Macurkova, J. Dvorak, Bioscience Reports 44 (2024).
date_created: 2025-12-12T09:03:54Z
date_published: 2024-01-12T00:00:00Z
date_updated: 2025-12-15T10:21:56Z
day: '12'
ddc:
- '570'
doi: 10.1042/bsr20230502
extern: '1'
external_id:
  pmid:
  - '38108122 '
fulldoi: https://doi.org/10.1042/bsr20230502
has_accepted_license: '1'
intvolume: '        44'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1042/BSR20230502
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Bioscience Reports
publication_identifier:
  eissn:
  - 1573-4935
  issn:
  - 0144-8463
publication_status: published
publisher: Portland Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Uncovering the essential roles of glutamate carboxypeptidase 2 orthologs in
  Caenorhabditis elegans
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 44
year: '2024'
...