@article{1795,
  abstract     = {Background: Murine leukemia virus (MLV) vector particles can be pseudotyped with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope protein (Env) and selectively target gene transfer to human cells expressing both CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY cells, which express the MLV gag and pol genes, we generated stable producer cell lines that express the HIV-1 envelope gene and a retroviral vector genome encoding the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed from a bicistronic vector which allowed the rapid selection of stable cell lines. A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env expression. Vectors generated by these producer cells displayed different sensitivity to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors are a valuable screening system for entry inhibitors or neutralizing antisera generated by vaccines.},
  author       = {Siegert, Sandra and Thaler, Sonja and Wagner, Ralf and Schnierle, Barbara},
  issn         = {1742-6405},
  journal      = {AIDS Research and Therapy},
  number       = {1},
  publisher    = {BioMed Central},
  title        = {{Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors}},
  doi          = {10.1186/1742-6405-2-7},
  volume       = {2},
  year         = {2005},
}