---
_id: '1795'
abstract:
- lang: eng
  text: 'Background: Murine leukemia virus (MLV) vector particles can be pseudotyped
    with a truncated variant of the human immunodeficiency virus type 1 (HIV-1) envelope
    protein (Env) and selectively target gene transfer to human cells expressing both
    CD4 and an appropriate co-receptor. Vector transduction mimics the HIV-1 entry
    process and is therefore a safe tool to study HIV-1 entry. Results: Using FLY
    cells, which express the MLV gag and pol genes, we generated stable producer cell
    lines that express the HIV-1 envelope gene and a retroviral vector genome encoding
    the green fluorescent protein (GFP). The BH10 or 89.6 P HIV-1 Env was expressed
    from a bicistronic vector which allowed the rapid selection of stable cell lines.
    A codon-usage-optimized synthetic env gene permitted high, Rev-independent Env
    expression. Vectors generated by these producer cells displayed different sensitivity
    to entry inhibitors. Conclusion: These data illustrate that MLV/HIV-1 vectors
    are a valuable screening system for entry inhibitors or neutralizing antisera
    generated by vaccines.'
article_processing_charge: No
article_type: original
author:
- first_name: Sandra
  full_name: Siegert, Sandra
  id: 36ACD32E-F248-11E8-B48F-1D18A9856A87
  last_name: Siegert
  orcid: 0000-0001-8635-0877
- first_name: Sonja
  full_name: Thaler, Sonja
  last_name: Thaler
- first_name: Ralf
  full_name: Wagner, Ralf
  last_name: Wagner
- first_name: Barbara
  full_name: Schnierle, Barbara
  last_name: Schnierle
citation:
  ama: Siegert S, Thaler S, Wagner R, Schnierle B. Assessment of HIV-1 entry inhibitors
    by MLV/HIV-1 pseudotyped vectors. <i>AIDS Research and Therapy</i>. 2005;2(1).
    doi:<a href="https://doi.org/10.1186/1742-6405-2-7">10.1186/1742-6405-2-7</a>
  apa: Siegert, S., Thaler, S., Wagner, R., &#38; Schnierle, B. (2005). Assessment
    of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors. <i>AIDS Research and
    Therapy</i>. BioMed Central. <a href="https://doi.org/10.1186/1742-6405-2-7">https://doi.org/10.1186/1742-6405-2-7</a>
  chicago: Siegert, Sandra, Sonja Thaler, Ralf Wagner, and Barbara Schnierle. “Assessment
    of HIV-1 Entry Inhibitors by MLV/HIV-1 Pseudotyped Vectors.” <i>AIDS Research
    and Therapy</i>. BioMed Central, 2005. <a href="https://doi.org/10.1186/1742-6405-2-7">https://doi.org/10.1186/1742-6405-2-7</a>.
  ieee: S. Siegert, S. Thaler, R. Wagner, and B. Schnierle, “Assessment of HIV-1 entry
    inhibitors by MLV/HIV-1 pseudotyped vectors,” <i>AIDS Research and Therapy</i>,
    vol. 2, no. 1. BioMed Central, 2005.
  ista: Siegert S, Thaler S, Wagner R, Schnierle B. 2005. Assessment of HIV-1 entry
    inhibitors by MLV/HIV-1 pseudotyped vectors. AIDS Research and Therapy. 2(1).
  mla: Siegert, Sandra, et al. “Assessment of HIV-1 Entry Inhibitors by MLV/HIV-1
    Pseudotyped Vectors.” <i>AIDS Research and Therapy</i>, vol. 2, no. 1, BioMed
    Central, 2005, doi:<a href="https://doi.org/10.1186/1742-6405-2-7">10.1186/1742-6405-2-7</a>.
  short: S. Siegert, S. Thaler, R. Wagner, B. Schnierle, AIDS Research and Therapy
    2 (2005).
das_tickbox: '0'
date_created: 2018-12-11T11:54:03Z
date_published: 2005-09-12T00:00:00Z
date_updated: 2026-08-07T12:29:10Z
day: '12'
doi: 10.1186/1742-6405-2-7
extern: '1'
external_id:
  pmid:
  - '16156891'
intvolume: '         2'
issue: '1'
language:
- iso: eng
month: '09'
oa_version: None
pmid: 1
publication: AIDS Research and Therapy
publication_identifier:
  eissn:
  - 1742-6405
publication_status: published
publisher: BioMed Central
publist_id: '5315'
status: public
title: Assessment of HIV-1 entry inhibitors by MLV/HIV-1 pseudotyped vectors
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 2
year: '2005'
...