@article{22301, abstract = {Auxin, primarily indole-3-acetic acid (IAA), is a central regulator of growth and development in land plants, but its physiological role in chlorophyte algae remains unclear. Here, we show that exogenous IAA modulates growth in Chlorella sorokiniana, Chlorella variabilis, and Chlamydomonas reinhardtii in a concentration-dependent manner. Low IAA concentrations promoted growth by accelerating the onset of cell division without affecting cell size, whereas higher concentrations inhibited proliferation. Radiotracer assays showed that all three species take up and release IAA across the plasma membrane through a combination of passive diffusion and energy-dependent, saturable processes. Competition by excess unlabeled natural and synthetic auxins further supported the presence of carrier-mediated transport with broad substrate recognition. Phylogenetic analyses identified potential PIN-like auxin exporters in chlorophytes and other non-plant eukaryotes, and structural modeling supported conservation of the overall PIN fold and predicted auxin-binding residues. However, functional assays in Xenopus laevis oocytes, tobacco BY-2 cultured cells, and Arabidopsis thaliana did not support a role for these proteins in directional auxin export. Instead, non-plant PIN homologs localized predominantly to the endoplasmic reticulum and showed limited or no transport activity in heterologous systems. Together, these findings indicate that auxin responsiveness and basic cellular auxin transport predate canonical PIN-mediated directional auxin export, which appears to be a later innovation of the streptophyte lineage.}, author = {Smoljan, Adrijana and Koutnik‐Abele, Sarah and Vladimirtsev, Dmitrii and Klíma, Petr and Bírošíková, Anita and Zhang, Yuzhou and Merrin, Jack and Schuster, Maximilian and Kurtović, Katarina and Hammes, Ulrich Z. and Petrášek, Jan and Friml, Jiří}, issn = {1744-7909}, journal = {Journal of Integrative Plant Biology}, publisher = {Wiley}, title = {{Auxin response and PIN‐mediated transport in chlorophyte algae}}, doi = {10.1111/jipb.70309}, year = {2026}, } @article{12670, abstract = {DNA methylation plays essential homeostatic functions in eukaryotic genomes. In animals, DNA methylation is also developmentally regulated and, in turn, regulates development. In the past two decades, huge research effort has endorsed the understanding that DNA methylation plays a similar role in plant development, especially during sexual reproduction. The power of whole-genome sequencing and cell isolation techniques, as well as bioinformatics tools, have enabled recent studies to reveal dynamic changes in DNA methylation during germline development. Furthermore, the combination of these technological advances with genetics, developmental biology and cell biology tools has revealed functional methylation reprogramming events that control gene and transposon activities in flowering plant germlines. In this review, we discuss the major advances in our knowledge of DNA methylation dynamics during male and female germline development in flowering plants.}, author = {He, Shengbo and Feng, Xiaoqi}, issn = {1744-7909}, journal = {Journal of Integrative Plant Biology}, keywords = {Plant Science, General Biochemistry, Genetics and Molecular Biology, Biochemistry}, number = {12}, pages = {2240--2251}, publisher = {Wiley}, title = {{DNA methylation dynamics during germline development}}, doi = {10.1111/jipb.13422}, volume = {64}, year = {2022}, } @article{10719, abstract = {Auxin, one of the first identified and most widely studied phytohormones, has been and will remain a hot topic in plant biology. After more than a century of passionate exploration, the mysteries of its synthesis, transport, signaling, and metabolism have largely been unlocked. Due to the rapid development of new technologies, new methods, and new genetic materials, the study of auxin has entered the fast lane over the past 30 years. Here, we highlight advances in understanding auxin signaling, including auxin perception, rapid auxin responses, TRANSPORT INHIBITOR RESPONSE 1 and AUXIN SIGNALING F-boxes (TIR1/AFBs)-mediated transcriptional and non-transcriptional branches, and the epigenetic regulation of auxin signaling. We also focus on feedback inhibition mechanisms that prevent the over-amplification of auxin signals. In addition, we cover the TRANSMEMBRANE KINASEs (TMKs)-mediated non-canonical signaling, which converges with TIR1/AFBs-mediated transcriptional regulation to coordinate plant growth and development. The identification of additional auxin signaling components and their regulation will continue to open new avenues of research in this field, leading to an increasingly deeper, more comprehensive understanding of how auxin signals are interpreted at the cellular level to regulate plant growth and development.}, author = {Yu, Z and Zhang, F and Friml, Jiří and Ding, Z}, issn = {1744-7909}, journal = {Journal of Integrative Plant Biology}, number = {2}, pages = {371--392}, publisher = {Wiley}, title = {{Auxin signaling: Research advances over the past 30 years}}, doi = {10.1111/jipb.13225}, volume = {64}, year = {2022}, } @article{7497, abstract = {Endophytic fungi can be beneficial to plant growth. However, the molecular mechanisms underlying colonization of Acremonium spp. remain unclear. In this study, a novel endophytic Acremonium strain was isolated from the buds of Panax notoginseng and named Acremonium sp. D212. The Acremonium sp. D212 could colonize the roots of P. notoginseng, enhance the resistance of P. notoginseng to root rot disease, and promote root growth and saponin biosynthesis in P. notoginseng. Acremonium sp. D212 could secrete indole‐3‐acetic acid (IAA) and jasmonic acid (JA), and inoculation with the fungus increased the endogenous levels of IAA and JA in P. notoginseng. Colonization of the Acremonium sp. D212 in the roots of the rice line Nipponbare was dependent on the concentration of methyl jasmonate (MeJA) (2 to 15 μM) and 1‐naphthalenacetic acid (NAA) (10 to 20 μM). Moreover, the roots of the JA signalling‐defective coi1‐18 mutant were colonized by Acremonium sp. D212 to a lesser degree than those of the wild‐type Nipponbare and miR393b‐overexpressing lines, and the colonization was rescued by MeJA but not by NAA. It suggests that the cross‐talk between JA signalling and the auxin biosynthetic pathway plays a crucial role in the colonization of Acremonium sp. D212 in host plants.}, author = {Han, L and Zhou, X and Zhao, Y and Zhu, S and Wu, L and He, Y and Ping, X and Lu, X and Huang, W and Qian, J and Zhang, L and Jiang, X and Zhu, D and Luo, C and Li, S and Dong, Q and Fu, Q and Deng, K and Wang, X and Wang, L and Peng, S and Wu, J and Li, W and Friml, Jiří and Zhu, Y and He, X and Du, Y}, issn = {1744-7909}, journal = {Journal of Integrative Plant Biology}, number = {9}, pages = {1433--1451}, publisher = {Wiley}, title = {{Colonization of endophyte Acremonium sp. D212 in Panax notoginseng and rice mediated by auxin and jasmonic acid}}, doi = {10.1111/jipb.12905}, volume = {62}, year = {2020}, }