---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '23133'
abstract:
- lang: eng
  text: "Background\r\nCotyledon micrografting represents a useful tool for studying
    the central role of cotyledons during early plant development, especially their
    interplay with other plant organs with regard to long distance transport. While
    hypocotyl micrografting methods are well-established, cotyledon micrografting
    is still inefficient. By optimizing cotyledon micrografting, we aim for higher
    success rates and increased throughput in the model species Arabidopsis thaliana.\r\n\r\nResults\r\nWe
    established a cut and paste cotyledon surgery procedure on a flat and solid but
    moist surface which improved handling of small seedlings. By applying a specific
    cutting and joining pattern, throughput was increased up to 40 seedlings per hour.
    The combination of short-day photoperiods and low light intensities for germination
    and long days plus high light intensities, elevated temperature and vertical plate
    positioning after grafting significantly increased ‘ligation’ efficiency. In particular
    high temperatures affected success rates favorably. Altogether, we achieved up
    to 92% grafting success in A. thaliana. Reconnection of vasculature was demonstrated
    by transport of a vasculature-specific dye across the grafting site. Phloem and
    xylem reconnection were completed 3–4 and 4–6 days after grafting, respectively,
    in a temperature-dependent manner. We observed that plants with grafted cotyledons
    match plants with intact cotyledons in biomass production and rosette development.\r\n\r\nConclusions\r\nThis
    cut and paste cotyledon-to-petiole micrografting protocol simplifies the handling
    of plant seedlings in surgery, increases the number of grafted plants per hour
    and greatly improves success rates for A. thaliana seedlings. The developed cotyledon
    micrografting method is also suitable for other plant species of comparable size."
article_number: '12'
article_processing_charge: Yes
article_type: original
author:
- first_name: Kai
  full_name: Bartusch, Kai
  id: 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
  last_name: Bartusch
  orcid: 0000-0003-2708-5545
- first_name: Jana
  full_name: Trenner, Jana
  last_name: Trenner
- first_name: Charles W.
  full_name: Melnyk, Charles W.
  last_name: Melnyk
- first_name: Marcel
  full_name: Quint, Marcel
  last_name: Quint
citation:
  ama: 'Bartusch K, Trenner J, Melnyk CW, Quint M. Cut and paste: Temperature-enhanced
    cotyledon micrografting for Arabidopsis thaliana seedlings. <i>Plant Methods</i>.
    2020;16. doi:<a href="https://doi.org/10.1186/s13007-020-0562-1">10.1186/s13007-020-0562-1</a>'
  apa: 'Bartusch, K., Trenner, J., Melnyk, C. W., &#38; Quint, M. (2020). Cut and
    paste: Temperature-enhanced cotyledon micrografting for Arabidopsis thaliana seedlings.
    <i>Plant Methods</i>. Springer. <a href="https://doi.org/10.1186/s13007-020-0562-1">https://doi.org/10.1186/s13007-020-0562-1</a>'
  chicago: 'Bartusch, Kai, Jana Trenner, Charles W. Melnyk, and Marcel Quint. “Cut
    and Paste: Temperature-Enhanced Cotyledon Micrografting for Arabidopsis Thaliana
    Seedlings.” <i>Plant Methods</i>. Springer, 2020. <a href="https://doi.org/10.1186/s13007-020-0562-1">https://doi.org/10.1186/s13007-020-0562-1</a>.'
  ieee: 'K. Bartusch, J. Trenner, C. W. Melnyk, and M. Quint, “Cut and paste: Temperature-enhanced
    cotyledon micrografting for Arabidopsis thaliana seedlings,” <i>Plant Methods</i>,
    vol. 16. Springer, 2020.'
  ista: 'Bartusch K, Trenner J, Melnyk CW, Quint M. 2020. Cut and paste: Temperature-enhanced
    cotyledon micrografting for Arabidopsis thaliana seedlings. Plant Methods. 16,
    12.'
  mla: 'Bartusch, Kai, et al. “Cut and Paste: Temperature-Enhanced Cotyledon Micrografting
    for Arabidopsis Thaliana Seedlings.” <i>Plant Methods</i>, vol. 16, 12, Springer,
    2020, doi:<a href="https://doi.org/10.1186/s13007-020-0562-1">10.1186/s13007-020-0562-1</a>.'
  short: K. Bartusch, J. Trenner, C.W. Melnyk, M. Quint, Plant Methods 16 (2020).
date_created: 2026-10-13T11:15:29Z
date_published: 2020-02-05T00:00:00Z
date_updated: 2026-10-14T12:25:45Z
day: '05'
ddc:
- '580'
doi: 10.1186/s13007-020-0562-1
extern: '1'
external_id:
  pmid:
  - '32042304'
fulldoi: https://doi.org/10.1186/s13007-020-0562-1
has_accepted_license: '1'
intvolume: '        16'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '02'
oa_version: None
pmid: 1
publication: Plant Methods
publication_identifier:
  eissn:
  - 1746-4811
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Cut and paste: Temperature-enhanced cotyledon micrografting for Arabidopsis
  thaliana seedlings'
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 16
year: '2020'
...