---
OA_place: publisher
OA_type: free access
_id: '21111'
abstract:
- lang: eng
  text: Inhibiting PLA2 activity should, in theory, be an effective approach to control
    the inflammation. Several naturally occurring polyphenolic compounds have been
    reported as inhibitors of PLA2. Among the naturally occurring polyphenols, catechol
    (1,2-dihydroxybenzene) possesses anti-inflammatory activity. Catechol can inhibit
    cyclooxygenase and lipo-oxygenase. By means of enzyme kinetic study, it was revealed
    that catechol can inhibit PLA2 also. Crystal structure showed that catechol binds
    to PLA2 at the opening of the active site cleft. This might stop the entry of
    substrate into the active site. Hence, catechol can be used as a lead compound
    for the development of novel anti-inflammatory drugs with PLA2 as the target.
article_processing_charge: No
article_type: original
author:
- first_name: Kalarickal V.
  full_name: Dileep, Kalarickal V.
  last_name: Dileep
- first_name: Ignatius
  full_name: Tintu, Ignatius
  last_name: Tintu
- first_name: Pradeep K
  full_name: Mandal, Pradeep K
  id: 6a3def15-d4b4-11ef-9fa9-a24c1f545ec3
  last_name: Mandal
  orcid: 0000-0001-5996-956X
- first_name: Ponnuraj
  full_name: Karthe, Ponnuraj
  last_name: Karthe
- first_name: Madathilkovilakathu
  full_name: Haridas, Madathilkovilakathu
  last_name: Haridas
- first_name: Chittalakkottu
  full_name: Sadasivan, Chittalakkottu
  last_name: Sadasivan
citation:
  ama: Dileep KV, Tintu I, Mandal PK, Karthe P, Haridas M, Sadasivan C. Binding to
    PLA2 may contribute to the anti‐inflammatory activity of catechol. <i>Chemical
    Biology &#38; Drug Design</i>. 2012;79(1):143-147. doi:<a href="https://doi.org/10.1111/j.1747-0285.2011.01258.x">10.1111/j.1747-0285.2011.01258.x</a>
  apa: Dileep, K. V., Tintu, I., Mandal, P. K., Karthe, P., Haridas, M., &#38; Sadasivan,
    C. (2012). Binding to PLA2 may contribute to the anti‐inflammatory activity of
    catechol. <i>Chemical Biology &#38; Drug Design</i>. Wiley. <a href="https://doi.org/10.1111/j.1747-0285.2011.01258.x">https://doi.org/10.1111/j.1747-0285.2011.01258.x</a>
  chicago: Dileep, Kalarickal V., Ignatius Tintu, Pradeep K Mandal, Ponnuraj Karthe,
    Madathilkovilakathu Haridas, and Chittalakkottu Sadasivan. “Binding to PLA2 May
    Contribute to the Anti‐inflammatory Activity of Catechol.” <i>Chemical Biology
    &#38; Drug Design</i>. Wiley, 2012. <a href="https://doi.org/10.1111/j.1747-0285.2011.01258.x">https://doi.org/10.1111/j.1747-0285.2011.01258.x</a>.
  ieee: K. V. Dileep, I. Tintu, P. K. Mandal, P. Karthe, M. Haridas, and C. Sadasivan,
    “Binding to PLA2 may contribute to the anti‐inflammatory activity of catechol,”
    <i>Chemical Biology &#38; Drug Design</i>, vol. 79, no. 1. Wiley, pp. 143–147,
    2012.
  ista: Dileep KV, Tintu I, Mandal PK, Karthe P, Haridas M, Sadasivan C. 2012. Binding
    to PLA2 may contribute to the anti‐inflammatory activity of catechol. Chemical
    Biology &#38; Drug Design. 79(1), 143–147.
  mla: Dileep, Kalarickal V., et al. “Binding to PLA2 May Contribute to the Anti‐inflammatory
    Activity of Catechol.” <i>Chemical Biology &#38; Drug Design</i>, vol. 79, no.
    1, Wiley, 2012, pp. 143–47, doi:<a href="https://doi.org/10.1111/j.1747-0285.2011.01258.x">10.1111/j.1747-0285.2011.01258.x</a>.
  short: K.V. Dileep, I. Tintu, P.K. Mandal, P. Karthe, M. Haridas, C. Sadasivan,
    Chemical Biology &#38; Drug Design 79 (2012) 143–147.
date_created: 2026-01-29T22:12:53Z
date_published: 2012-01-01T00:00:00Z
date_updated: 2026-02-20T08:20:52Z
day: '01'
doi: 10.1111/j.1747-0285.2011.01258.x
extern: '1'
external_id:
  pmid:
  - '21995306'
fulldoi: https://doi.org/10.1111/j.1747-0285.2011.01258.x
has_accepted_license: '1'
intvolume: '        79'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1111/j.1747-0285.2011.01258.x
month: '01'
oa: 1
oa_version: Published Version
page: 143-147
pmid: 1
publication: Chemical Biology & Drug Design
publication_identifier:
  eissn:
  - 1747-0285
  issn:
  - 1747-0277
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: Binding to PLA2 may contribute to the anti‐inflammatory activity of catechol
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 79
year: '2012'
...
---
OA_type: free access
_id: '21112'
abstract:
- lang: eng
  text: <jats:p>Ester bond hydrolysis of membrane phospholipids by Phospholipase A2
    and consequent release of fatty acids are the initiating steps of inflammation.
    It is proposed in this study that the inhibition of phospholipase A2 is one of
    the ways to control inflammation. Investigations are carried out to identify the
    mode of inhibition of phospholipase A2 by the n‐hexadecanoic acid. It may help
    in designing of specific inhibitors of phospholipase A2 as anti‐inflammatory agents.
    The enzyme kinetics study proved that n‐hexadecanoic acid inhibits phospholipase
    A2 in a competitive manner. It was identified from the crystal structure at 2.5Å
    resolution that the position of n‐hexadecanoic acid is in the active site of the
    phospholipase A2. The binding constant and binding energy have also been calculated
    using Isothermal Titration Calorimetry. Also, the binding energy of n‐hexadecanoic
    acid to phospholipase A2 was calculated by in silico method and compared with
    known inhibitors. It may be concluded from the structural and kinetics studies
    that the fatty acid, n‐hexadecanoic acid, is an inhibitor of phospholipase A2,
    hence, an anti‐inflammatory compound. The inferences from the present study validate
    the rigorous use of medicated oils rich in n‐hexadecanoic acid for the treatment
    of rheumatic symptoms in the traditional medical system of India, Ayurveda.
article_processing_charge: No
article_type: original
author:
- first_name: Vasudevan
  full_name: Aparna, Vasudevan
  last_name: Aparna
- first_name: Kalarickal V.
  full_name: Dileep, Kalarickal V.
  last_name: Dileep
- first_name: Pradeep K
  full_name: Mandal, Pradeep K
  id: 6a3def15-d4b4-11ef-9fa9-a24c1f545ec3
  last_name: Mandal
  orcid: 0000-0001-5996-956X
- first_name: Ponnuraj
  full_name: Karthe, Ponnuraj
  last_name: Karthe
- first_name: Chittalakkottu
  full_name: Sadasivan, Chittalakkottu
  last_name: Sadasivan
- first_name: Madathilkovilakathu
  full_name: Haridas, Madathilkovilakathu
  last_name: Haridas
citation:
  ama: 'Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M. Anti-inflammatory
    property of n-hexadecanoic acid: Structural evidence and kinetic assessment. <i>Chemical
    Biology &#38; Drug Design</i>. 2012;80(3):434-439. doi:<a href="https://doi.org/10.1111/j.1747-0285.2012.01418.x">10.1111/j.1747-0285.2012.01418.x</a>'
  apa: 'Aparna, V., Dileep, K. V., Mandal, P. K., Karthe, P., Sadasivan, C., &#38;
    Haridas, M. (2012). Anti-inflammatory property of n-hexadecanoic acid: Structural
    evidence and kinetic assessment. <i>Chemical Biology &#38; Drug Design</i>. Wiley.
    <a href="https://doi.org/10.1111/j.1747-0285.2012.01418.x">https://doi.org/10.1111/j.1747-0285.2012.01418.x</a>'
  chicago: 'Aparna, Vasudevan, Kalarickal V. Dileep, Pradeep K Mandal, Ponnuraj Karthe,
    Chittalakkottu Sadasivan, and Madathilkovilakathu Haridas. “Anti-Inflammatory
    Property of n-Hexadecanoic Acid: Structural Evidence and Kinetic Assessment.”
    <i>Chemical Biology &#38; Drug Design</i>. Wiley, 2012. <a href="https://doi.org/10.1111/j.1747-0285.2012.01418.x">https://doi.org/10.1111/j.1747-0285.2012.01418.x</a>.'
  ieee: 'V. Aparna, K. V. Dileep, P. K. Mandal, P. Karthe, C. Sadasivan, and M. Haridas,
    “Anti-inflammatory property of n-hexadecanoic acid: Structural evidence and kinetic
    assessment,” <i>Chemical Biology &#38; Drug Design</i>, vol. 80, no. 3. Wiley,
    pp. 434–439, 2012.'
  ista: 'Aparna V, Dileep KV, Mandal PK, Karthe P, Sadasivan C, Haridas M. 2012. Anti-inflammatory
    property of n-hexadecanoic acid: Structural evidence and kinetic assessment. Chemical
    Biology &#38; Drug Design. 80(3), 434–439.'
  mla: 'Aparna, Vasudevan, et al. “Anti-Inflammatory Property of n-Hexadecanoic Acid:
    Structural Evidence and Kinetic Assessment.” <i>Chemical Biology &#38; Drug Design</i>,
    vol. 80, no. 3, Wiley, 2012, pp. 434–39, doi:<a href="https://doi.org/10.1111/j.1747-0285.2012.01418.x">10.1111/j.1747-0285.2012.01418.x</a>.'
  short: V. Aparna, K.V. Dileep, P.K. Mandal, P. Karthe, C. Sadasivan, M. Haridas,
    Chemical Biology &#38; Drug Design 80 (2012) 434–439.
date_created: 2026-01-29T22:14:04Z
date_published: 2012-09-01T00:00:00Z
date_updated: 2026-02-20T08:23:29Z
day: '01'
doi: 10.1111/j.1747-0285.2012.01418.x
extern: '1'
external_id:
  pmid:
  - '22642495'
fulldoi: https://doi.org/10.1111/j.1747-0285.2012.01418.x
has_accepted_license: '1'
intvolume: '        80'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1111/j.1747-0285.2012.01418.x
month: '09'
oa: 1
oa_version: Published Version
page: 434-439
pmid: 1
publication: Chemical Biology & Drug Design
publication_identifier:
  eissn:
  - 1747-0285
  issn:
  - 1747-0277
publication_status: published
publisher: Wiley
quality_controlled: '1'
status: public
title: 'Anti-inflammatory property of n-hexadecanoic acid: Structural evidence and
  kinetic assessment'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 80
year: '2012'
...