---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '21779'
abstract:
- lang: eng
  text: Acidomycin is an anti-mycobacterial antibiotic with a unique mode of action,
    targeting the biotin biosynthesis pathway. Despite being highly active against
    mycobacteria in vitro, its development as an anti-tubercular agent has been hindered
    due to suboptimal pharmacokinetics. Engineering of the acidomycin biosynthesis
    may yield new analogues with improved pharmacological properties. Here, we describe
    the identification of the acidomycin biosynthetic gene cluster (BGC) in a Streptomyces
    bacterium isolated from the rhizosphere of Edelweiss. Notably, the acidomycin
    BGC is located in proximity to the genes for the biosynthesis of stravidins, secondary
    metabolites targeting a different enzyme in the biotin biosynthesis pathway, and
    two genes for streptavidins, proteins that strongly bind and sequester biotin.
    The identity of the acidomycin BGC was confirmed via both gene knock-out and heterologous
    expression, which suggested that the fatty acid required for the formation of
    acidomycin's acyl chain is most likely scavenged from the biotin biosynthesis
    pathway. CRISPR/Cas9-assisted knock-out of the cytochrome P450-encoding gene in
    the acidomycin BGC resulted in a significant decrease in its yield but did not
    abrogate the biosynthesis completely.
acknowledgement: This work was supported by the University of Vienna. The authors
  thank Anna Fabisikova from the Mass Spectrometry Centre and the team of the NMR
  Centre (both of the Faculty of Chemistry, University of Vienna and members of the
  Vienna Life Science Instruments) for assistance with data acquisition. Open Access
  funding provided by Universitat Wien. This work was supported by Universität Wien.
article_number: e70357
article_processing_charge: Yes
article_type: original
author:
- first_name: Anna
  full_name: Vignolle, Anna
  last_name: Vignolle
- first_name: Martin
  full_name: Zehl, Martin
  id: 8e016d5b-5d77-11f0-86d2-96cdb3922a55
  last_name: Zehl
  orcid: 0000-0001-9685-0373
- first_name: Jaime Felipe Guerrero
  full_name: Garzón, Jaime Felipe Guerrero
  last_name: Garzón
- first_name: Olha
  full_name: Schneider, Olha
  last_name: Schneider
- first_name: Johannes
  full_name: Gafriller, Johannes
  last_name: Gafriller
- first_name: Ulrike
  full_name: Grienke, Ulrike
  last_name: Grienke
- first_name: Rasmus H.
  full_name: Kirkegaard, Rasmus H.
  last_name: Kirkegaard
- first_name: Sergey B.
  full_name: Zotchev, Sergey B.
  last_name: Zotchev
citation:
  ama: Vignolle A, Zehl M, Garzón JFG, et al. Identification and characterisation
    of the gene cluster governing biosynthesis of the anti-mycobacterial antibiotic
    acidomycin. <i>Microbial Biotechnology</i>. 2026;19(4). doi:<a href="https://doi.org/10.1111/1751-7915.70357">10.1111/1751-7915.70357</a>
  apa: Vignolle, A., Zehl, M., Garzón, J. F. G., Schneider, O., Gafriller, J., Grienke,
    U., … Zotchev, S. B. (2026). Identification and characterisation of the gene cluster
    governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. <i>Microbial
    Biotechnology</i>. Wiley. <a href="https://doi.org/10.1111/1751-7915.70357">https://doi.org/10.1111/1751-7915.70357</a>
  chicago: Vignolle, Anna, Martin Zehl, Jaime Felipe Guerrero Garzón, Olha Schneider,
    Johannes Gafriller, Ulrike Grienke, Rasmus H. Kirkegaard, and Sergey B. Zotchev.
    “Identification and Characterisation of the Gene Cluster Governing Biosynthesis
    of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial Biotechnology</i>.
    Wiley, 2026. <a href="https://doi.org/10.1111/1751-7915.70357">https://doi.org/10.1111/1751-7915.70357</a>.
  ieee: A. Vignolle <i>et al.</i>, “Identification and characterisation of the gene
    cluster governing biosynthesis of the anti-mycobacterial antibiotic acidomycin,”
    <i>Microbial Biotechnology</i>, vol. 19, no. 4. Wiley, 2026.
  ista: Vignolle A, Zehl M, Garzón JFG, Schneider O, Gafriller J, Grienke U, Kirkegaard
    RH, Zotchev SB. 2026. Identification and characterisation of the gene cluster
    governing biosynthesis of the anti-mycobacterial antibiotic acidomycin. Microbial
    Biotechnology. 19(4), e70357.
  mla: Vignolle, Anna, et al. “Identification and Characterisation of the Gene Cluster
    Governing Biosynthesis of the Anti-Mycobacterial Antibiotic Acidomycin.” <i>Microbial
    Biotechnology</i>, vol. 19, no. 4, e70357, Wiley, 2026, doi:<a href="https://doi.org/10.1111/1751-7915.70357">10.1111/1751-7915.70357</a>.
  short: A. Vignolle, M. Zehl, J.F.G. Garzón, O. Schneider, J. Gafriller, U. Grienke,
    R.H. Kirkegaard, S.B. Zotchev, Microbial Biotechnology 19 (2026).
date_created: 2026-05-03T22:01:37Z
date_published: 2026-04-01T00:00:00Z
date_updated: 2026-05-07T08:22:41Z
day: '01'
ddc:
- '570'
department:
- _id: MassSpec
doi: 10.1111/1751-7915.70357
external_id:
  pmid:
  - '42036976'
file:
- access_level: open_access
  checksum: 8c8aa660cef5394167e06f187adbabf0
  content_type: application/pdf
  creator: dernst
  date_created: 2026-05-07T08:21:06Z
  date_updated: 2026-05-07T08:21:06Z
  file_id: '21835'
  file_name: 2026_MicrobialBiotechnology_Vignolle.pdf
  file_size: 575492
  relation: main_file
  success: 1
file_date_updated: 2026-05-07T08:21:06Z
has_accepted_license: '1'
intvolume: '        19'
issue: '4'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: Microbial Biotechnology
publication_identifier:
  eissn:
  - 1751-7915
publication_status: published
publisher: Wiley
quality_controlled: '1'
scopus_import: '1'
status: public
title: Identification and characterisation of the gene cluster governing biosynthesis
  of the anti-mycobacterial antibiotic acidomycin
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 19
year: '2026'
...