[{"quality_controlled":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"article_number":"146458","article_processing_charge":"No","publication_identifier":{"issn":["0006-8993"],"eissn":["1872-6240"]},"date_updated":"2025-07-10T11:54:01Z","title":"Modeling cell-cell interactions in the brain using cerebral organoids","pmid":1,"department":[{"_id":"GaNo"}],"scopus_import":"1","isi":1,"publication":"Brain Research","citation":{"short":"B. Oliveira, A.Ç. Yahya, G. Novarino, Brain Research 1724 (2019).","chicago":"Oliveira, Bárbara, Aysan Çerağ Yahya, and Gaia Novarino. “Modeling Cell-Cell Interactions in the Brain Using Cerebral Organoids.” <i>Brain Research</i>. Elsevier, 2019. <a href=\"https://doi.org/10.1016/j.brainres.2019.146458\">https://doi.org/10.1016/j.brainres.2019.146458</a>.","mla":"Oliveira, Bárbara, et al. “Modeling Cell-Cell Interactions in the Brain Using Cerebral Organoids.” <i>Brain Research</i>, vol. 1724, 146458, Elsevier, 2019, doi:<a href=\"https://doi.org/10.1016/j.brainres.2019.146458\">10.1016/j.brainres.2019.146458</a>.","ista":"Oliveira B, Yahya AÇ, Novarino G. 2019. Modeling cell-cell interactions in the brain using cerebral organoids. Brain Research. 1724, 146458.","ama":"Oliveira B, Yahya AÇ, Novarino G. Modeling cell-cell interactions in the brain using cerebral organoids. <i>Brain Research</i>. 2019;1724. doi:<a href=\"https://doi.org/10.1016/j.brainres.2019.146458\">10.1016/j.brainres.2019.146458</a>","apa":"Oliveira, B., Yahya, A. Ç., &#38; Novarino, G. (2019). Modeling cell-cell interactions in the brain using cerebral organoids. <i>Brain Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.brainres.2019.146458\">https://doi.org/10.1016/j.brainres.2019.146458</a>","ieee":"B. Oliveira, A. Ç. Yahya, and G. Novarino, “Modeling cell-cell interactions in the brain using cerebral organoids,” <i>Brain Research</i>, vol. 1724. Elsevier, 2019."},"status":"public","publisher":"Elsevier","_id":"6896","intvolume":"      1724","type":"journal_article","doi":"10.1016/j.brainres.2019.146458","author":[{"last_name":"Oliveira","first_name":"Bárbara","id":"3B03AA1A-F248-11E8-B48F-1D18A9856A87","full_name":"Oliveira, Bárbara"},{"full_name":"Yahya, Aysan Çerağ","id":"365A65F8-F248-11E8-B48F-1D18A9856A87","first_name":"Aysan Çerağ","last_name":"Yahya"},{"first_name":"Gaia","last_name":"Novarino","full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-7673-7178"}],"date_created":"2019-09-22T22:00:35Z","month":"12","day":"01","external_id":{"pmid":["31521639"],"isi":["000491646600033"]},"oa_version":"None","article_type":"original","publication_status":"published","abstract":[{"text":"Until recently, a great amount of brain studies have been conducted in human post mortem tissues, cell lines and model organisms. These researches provided useful insights regarding cell-cell interactions occurring in the brain. However, such approaches suffer from technical limitations and inaccurate modeling of the tissue 3D cytoarchitecture. Importantly, they might lack a human genetic background essential for disease modeling. With the development of protocols to generate human cerebral organoids, we are now closer to reproducing the early stages of human brain development in vitro. As a result, more relevant cell-cell interaction studies can be conducted.\r\n\r\nIn this review, we discuss the advantages of 3D cultures over 2D in modulating brain cell-cell interactions during physiological and pathological development, as well as the progress made in developing organoids in which neurons, macroglia, microglia and vascularization are present. Finally, we debate the limitations of those models and possible future directions.","lang":"eng"}],"year":"2019","date_published":"2019-12-01T00:00:00Z","volume":1724},{"title":"Direct projections from Ammon's horn to the rostral raphe regions in the brainstem of the cat","date_updated":"2022-02-15T09:14:43Z","acknowledgement":"The photographic help of Mr. Akira Uesugi, and the support of Niwa Medical Research Foundation are gratefully acknowledged. This work has been supported in part by Grant-in-Aid for Special Project Research (No. 63112003) and Grant-in-Aid for Scientific Research (No. 62480098) from the Ministry of Education, Science and Culture of Japan.","publication_identifier":{"issn":["0006-8993"],"eissn":["1872-6240"]},"article_processing_charge":"No","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","language":[{"iso":"eng"}],"quality_controlled":"1","status":"public","page":"157 - 161","citation":{"ieee":"T. Ino <i>et al.</i>, “Direct projections from Ammon’s horn to the rostral raphe regions in the brainstem of the cat,” <i>Brain Research</i>, vol. 479, no. 1. Elsevier, pp. 157–161, 1989.","ama":"Ino T, Itoh K, Kamiya H, et al. Direct projections from Ammon’s horn to the rostral raphe regions in the brainstem of the cat. <i>Brain Research</i>. 1989;479(1):157-161. doi:<a href=\"https://doi.org/10.1016/0006-8993(89)91346-2\">10.1016/0006-8993(89)91346-2</a>","apa":"Ino, T., Itoh, K., Kamiya, H., Kaneko, T., Shigemoto, R., Akiguchi, I., &#38; Mizuno, N. (1989). Direct projections from Ammon’s horn to the rostral raphe regions in the brainstem of the cat. <i>Brain Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/0006-8993(89)91346-2\">https://doi.org/10.1016/0006-8993(89)91346-2</a>","mla":"Ino, Tadashi, et al. “Direct Projections from Ammon’s Horn to the Rostral Raphe Regions in the Brainstem of the Cat.” <i>Brain Research</i>, vol. 479, no. 1, Elsevier, 1989, pp. 157–61, doi:<a href=\"https://doi.org/10.1016/0006-8993(89)91346-2\">10.1016/0006-8993(89)91346-2</a>.","ista":"Ino T, Itoh K, Kamiya H, Kaneko T, Shigemoto R, Akiguchi I, Mizuno N. 1989. Direct projections from Ammon’s horn to the rostral raphe regions in the brainstem of the cat. Brain Research. 479(1), 157–161.","chicago":"Ino, Tadashi, Kazuo Itoh, Hiroto Kamiya, Takeshi Kaneko, Ryuichi Shigemoto, Ichiro Akiguchi, and Noboru Mizuno. “Direct Projections from Ammon’s Horn to the Rostral Raphe Regions in the Brainstem of the Cat.” <i>Brain Research</i>. Elsevier, 1989. <a href=\"https://doi.org/10.1016/0006-8993(89)91346-2\">https://doi.org/10.1016/0006-8993(89)91346-2</a>.","short":"T. Ino, K. Itoh, H. Kamiya, T. Kaneko, R. Shigemoto, I. Akiguchi, N. Mizuno, Brain Research 479 (1989) 157–161."},"publication":"Brain Research","scopus_import":"1","issue":"1","pmid":1,"author":[{"last_name":"Ino","first_name":"Tadashi","full_name":"Ino, Tadashi"},{"first_name":"Kazuo","last_name":"Itoh","full_name":"Itoh, Kazuo"},{"first_name":"Hiroto","last_name":"Kamiya","full_name":"Kamiya, Hiroto"},{"last_name":"Kaneko","first_name":"Takeshi","full_name":"Kaneko, Takeshi"},{"full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8761-9444","first_name":"Ryuichi","last_name":"Shigemoto"},{"full_name":"Akiguchi, Ichiro","last_name":"Akiguchi","first_name":"Ichiro"},{"last_name":"Mizuno","first_name":"Noboru","full_name":"Mizuno, Noboru"}],"date_created":"2018-12-11T11:58:11Z","doi":"10.1016/0006-8993(89)91346-2","intvolume":"       479","type":"journal_article","_id":"2526","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/0006899389913462?via%3Dihub"}],"publisher":"Elsevier","date_published":"1989-02-06T00:00:00Z","volume":479,"year":"1989","publist_id":"4373","abstract":[{"text":"When WGA-HRP (wheat germ agglutinin-horseradish peroxidase conjugate) or HRP was injected into the regions around the superior central and/or the dorsal raphe nuclei in the cat, cell bodies of a number of non-pyramidal neurons were labeled in Ammon's horn. Thus the existence of direct projections from non-pyramidal neurons in Ammon's horn to the rostral raphe regions in the brainstem was suggested in the cat.","lang":"eng"}],"publication_status":"published","article_type":"original","oa_version":"None","external_id":{"pmid":["2466535 "]},"day":"06","extern":"1","month":"02"},{"pmid":1,"issue":"1","scopus_import":"1","page":"173 - 177","citation":{"ieee":"T. Ino, K. Itoh, H. Kamiya, R. Shigemoto, I. Akiguchi, and N. Mizuno, “Direct projections of non-pyramidal neurons of Ammon’s horn to the supramammillary region in the cat,” <i>Brain Research</i>, vol. 460, no. 1. Elsevier, pp. 173–177, 1988.","ama":"Ino T, Itoh K, Kamiya H, Shigemoto R, Akiguchi I, Mizuno N. Direct projections of non-pyramidal neurons of Ammon’s horn to the supramammillary region in the cat. <i>Brain Research</i>. 1988;460(1):173-177. doi:<a href=\"https://doi.org/10.1016/0006-8993(88)91219-X\">10.1016/0006-8993(88)91219-X</a>","apa":"Ino, T., Itoh, K., Kamiya, H., Shigemoto, R., Akiguchi, I., &#38; Mizuno, N. (1988). Direct projections of non-pyramidal neurons of Ammon’s horn to the supramammillary region in the cat. <i>Brain Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/0006-8993(88)91219-X\">https://doi.org/10.1016/0006-8993(88)91219-X</a>","mla":"Ino, Tadashi, et al. “Direct Projections of Non-Pyramidal Neurons of Ammon’s Horn to the Supramammillary Region in the Cat.” <i>Brain Research</i>, vol. 460, no. 1, Elsevier, 1988, pp. 173–77, doi:<a href=\"https://doi.org/10.1016/0006-8993(88)91219-X\">10.1016/0006-8993(88)91219-X</a>.","ista":"Ino T, Itoh K, Kamiya H, Shigemoto R, Akiguchi I, Mizuno N. 1988. Direct projections of non-pyramidal neurons of Ammon’s horn to the supramammillary region in the cat. Brain Research. 460(1), 173–177.","chicago":"Ino, Tadashi, Kazuo Itoh, Hiroto Kamiya, Ryuichi Shigemoto, Ichiro Akiguchi, and Noboru Mizuno. “Direct Projections of Non-Pyramidal Neurons of Ammon’s Horn to the Supramammillary Region in the Cat.” <i>Brain Research</i>. Elsevier, 1988. <a href=\"https://doi.org/10.1016/0006-8993(88)91219-X\">https://doi.org/10.1016/0006-8993(88)91219-X</a>.","short":"T. Ino, K. Itoh, H. Kamiya, R. Shigemoto, I. Akiguchi, N. Mizuno, Brain Research 460 (1988) 173–177."},"publication":"Brain Research","status":"public","quality_controlled":"1","language":[{"iso":"eng"}],"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","article_processing_charge":"No","date_updated":"2022-02-08T14:38:35Z","acknowledgement":"The photographic help of Mr. Akira Uesugi and Mr. Kenichi Uesugi, and the support of the Niwa Medical Research \r\nFoundation are gratefully acknowledged. This work has also been supported in part by Grant-in-Aid for Scientific Research No. 62480098 from the Ministry of Education, Science and Culture of Japan.","publication_identifier":{"eissn":["1872-6240"],"issn":["0006-8993"]},"title":"Direct projections of non-pyramidal neurons of Ammon's horn to the supramammillary region in the cat","extern":"1","month":"09","day":"13","oa_version":"None","external_id":{"pmid":["2464404 "]},"abstract":[{"lang":"eng","text":"Non-pyramidal neurons in cat Ammon's horn were shown to send their axons to the supramammillary regions (SMR), i.e. the supramammillary nucleus and its vicinities including the supramammillary nucleus and the lateral, posterior and dorsal hypothalamic areas: wheat germ agglutinin-horseradish peroxidase (WGA-HRP) injection into Ammon's horn resulted in labeling of presumed axon terminals in the SMR; and after injecting HRP into the SMR, retrogradely labeled non-pyramidal neurons were seen in Ammon's horn."}],"publication_status":"published","article_type":"original","publist_id":"4376","volume":460,"date_published":"1988-09-13T00:00:00Z","year":"1988","publisher":"Elsevier","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/000689938891219X?via%3Dihub"}],"_id":"2522","type":"journal_article","intvolume":"       460","doi":"10.1016/0006-8993(88)91219-X","author":[{"full_name":"Ino, Tadashi","first_name":"Tadashi","last_name":"Ino"},{"last_name":"Itoh","first_name":"Kazuo","full_name":"Itoh, Kazuo"},{"full_name":"Kamiya, Hiroto","first_name":"Hiroto","last_name":"Kamiya"},{"orcid":"0000-0001-8761-9444","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","first_name":"Ryuichi"},{"full_name":"Akiguchi, Ichiro","last_name":"Akiguchi","first_name":"Ichiro"},{"first_name":"Noboru","last_name":"Mizuno","full_name":"Mizuno, Noboru"}],"date_created":"2018-12-11T11:58:10Z"},{"publist_id":"4375","publication_status":"published","article_type":"original","abstract":[{"lang":"eng","text":"Alpha-ketoglutamate (α-KG) reductive amination activity in rat brain was found to be mostly absorbed with an antibody against liver glutamate dehydrogenase. With this and anti-glutamine synthetase antibodies, α-KG reductive amination activity was immunocytochemically shown to coexist with glutamine synthetase activity in astrocytes. The results suggest that astrocytes de novo synthesize glutamate from α-KG and ammonia, and metabolize it to glutamine."}],"year":"1988","date_published":"1988-08-02T00:00:00Z","volume":457,"extern":"1","month":"08","external_id":{"pmid":["3167561"]},"oa_version":"None","day":"02","intvolume":"       457","type":"journal_article","date_created":"2018-12-11T11:58:11Z","author":[{"last_name":"Kaneko","first_name":"Takeshi","full_name":"Kaneko, Takeshi"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi"},{"first_name":"Noboru","last_name":"Mizuno","full_name":"Mizuno, Noboru"}],"doi":"10.1016/0006-8993(88)90069-8","_id":"2524","publisher":"Elsevier","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/0006899388900698?via%3Dihub"}],"publication":"Brain Research","page":"160 - 164","citation":{"short":"T. Kaneko, R. Shigemoto, N. Mizuno, Brain Research 457 (1988) 160–164.","chicago":"Kaneko, Takeshi, Ryuichi Shigemoto, and Noboru Mizuno. “Metabolism of Glutamate and Ammonia in Astrocyte an Immunocytochemical Study.” <i>Brain Research</i>. Elsevier, 1988. <a href=\"https://doi.org/10.1016/0006-8993(88)90069-8\">https://doi.org/10.1016/0006-8993(88)90069-8</a>.","mla":"Kaneko, Takeshi, et al. “Metabolism of Glutamate and Ammonia in Astrocyte an Immunocytochemical Study.” <i>Brain Research</i>, vol. 457, no. 1, Elsevier, 1988, pp. 160–64, doi:<a href=\"https://doi.org/10.1016/0006-8993(88)90069-8\">10.1016/0006-8993(88)90069-8</a>.","ista":"Kaneko T, Shigemoto R, Mizuno N. 1988. Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study. Brain Research. 457(1), 160–164.","ama":"Kaneko T, Shigemoto R, Mizuno N. Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study. <i>Brain Research</i>. 1988;457(1):160-164. doi:<a href=\"https://doi.org/10.1016/0006-8993(88)90069-8\">10.1016/0006-8993(88)90069-8</a>","apa":"Kaneko, T., Shigemoto, R., &#38; Mizuno, N. (1988). Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study. <i>Brain Research</i>. Elsevier. <a href=\"https://doi.org/10.1016/0006-8993(88)90069-8\">https://doi.org/10.1016/0006-8993(88)90069-8</a>","ieee":"T. Kaneko, R. Shigemoto, and N. Mizuno, “Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study,” <i>Brain Research</i>, vol. 457, no. 1. Elsevier, pp. 160–164, 1988."},"scopus_import":"1","status":"public","pmid":1,"issue":"1","article_processing_charge":"No","title":"Metabolism of glutamate and ammonia in astrocyte an immunocytochemical study","publication_identifier":{"issn":["0006-8993"],"eissn":["1872-6240"]},"date_updated":"2022-02-08T13:52:37Z","acknowledgement":"The authors are grateful to Akira Uesugi and Ken’ichi Uesugi for photographic help. This work was supported by the Niwa Medical Research Foundation and Grants-in-Aid for Special -Project Research (62121004), for General Scientific Research (62480098) and for Encouragement of Young Scientists (63770043) from the Ministry of Education, Science and Culture of Japan. ","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","language":[{"iso":"eng"}],"quality_controlled":"1"}]