[{"citation":{"apa":"Company-Garrido, I., Zurita Carpio, A., Colomer-Rosell, M., Ciraulo, B., Molkenbur, R., Lanzerstorfer, P., … Wieser, S. (2026). Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>","mla":"Company-Garrido, Iván, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>, vol. 61, no. 2, Elsevier, 2026, p. 356–371.e12, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>.","ieee":"I. Company-Garrido <i>et al.</i>, “Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses,” <i>Developmental Cell</i>, vol. 61, no. 2. Elsevier, p. 356–371.e12, 2026.","ista":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, Ciraulo B, Molkenbur R, Lanzerstorfer P, Pezzano F, Agazzi C, Hauschild R, Jain S, Jacques JM, Venturini V, Knapp C, Xie Y, Merrin J, Weghuber J, Schaaf M, Quidant R, Kiermaier E, Ortega Arroyo J, Ruprecht V, Wieser S. 2026. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. Developmental Cell. 61(2), 356–371.e12.","chicago":"Company-Garrido, Iván, Alberto Zurita Carpio, Mariona Colomer-Rosell, Bernard Ciraulo, Ronja Molkenbur, Peter Lanzerstorfer, Fabio Pezzano, et al. “Myosin II Regulates Cellular Thermo-Adaptability and the Efficiency of Immune Responses.” <i>Developmental Cell</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">https://doi.org/10.1016/j.devcel.2025.10.006</a>.","short":"I. Company-Garrido, A. Zurita Carpio, M. Colomer-Rosell, B. Ciraulo, R. Molkenbur, P. Lanzerstorfer, F. Pezzano, C. Agazzi, R. Hauschild, S. Jain, J.M. Jacques, V. Venturini, C. Knapp, Y. Xie, J. Merrin, J. Weghuber, M. Schaaf, R. Quidant, E. Kiermaier, J. Ortega Arroyo, V. Ruprecht, S. Wieser, Developmental Cell 61 (2026) 356–371.e12.","ama":"Company-Garrido I, Zurita Carpio A, Colomer-Rosell M, et al. Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses. <i>Developmental Cell</i>. 2026;61(2):356-371.e12. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.10.006\">10.1016/j.devcel.2025.10.006</a>"},"abstract":[{"text":"Effective immune responses rely on the efficient migration of leukocytes. Yet, how temperature regulates migration dynamics at the single-cell level has remained poorly understood. Using zebrafish embryos and mouse tissue explants, we found that temperature positively regulates leukocyte migration speed, exploration, and arrival frequencies to wounds and lymph vessels. Complementary 2D and 3D cultures revealed that this thermokinetic control of cell migration is conserved across immune cell types, independently of the 3D tissue environment. By applying precise (sub-)cellular temperature modulation, we identified a rapid and reversible thermo-response that depends on myosin II activity. Small physiological increases in temperature (1°C –2°C), as present during fever-like conditions, profoundly increased immune responses by accelerating arrival times at lymphatic vessels and tissue wounds. These findings identify myosin-II-dependent actomyosin contractility as a critical mechanical structure regulating single-cell thermo-adaptability, with physiological implications for tuning the speed of immune responses in vivo.","lang":"eng"}],"scopus_import":"1","doi":"10.1016/j.devcel.2025.10.006","has_accepted_license":"1","dataavailabilitystatement":"This study did not generate new unique reagents. Data are available upon request.\r\n•The custom-made codes used in this study are available at: https://github.com/mcolomerr/cell_thermo https://github.com/Stefan1980sol/Lymph_entry_simu\r\n• Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","PlanS_conform":"1","intvolume":"        61","publication_status":"published","supplementarymaterial":"yes","type":"journal_article","publisher":"Elsevier","language":[{"iso":"eng"}],"volume":61,"quality_controlled":"1","acknowledged_ssus":[{"_id":"NanoFab"}],"year":"2026","ddc":["570"],"month":"02","issue":"2","page":"356-371.e12","file_date_updated":"2026-07-23T06:26:25Z","article_processing_charge":"Yes (in subscription journal)","license":"https://creativecommons.org/licenses/by/4.0/","external_id":{"pmid":["41192429"]},"researchdata_availability":"upon request","oa":1,"date_published":"2026-02-11T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"OA_type":"hybrid","publication":"Developmental Cell","file":[{"access_level":"open_access","date_updated":"2026-07-23T06:26:25Z","success":1,"content_type":"application/pdf","file_id":"22388","file_name":"2026_DevelopmentalCell_CompanyGarrido.pdf","creator":"dernst","date_created":"2026-07-23T06:26:25Z","checksum":"52fd52d2d19a4514f8fcc1b40f420ca2","file_size":12342817,"relation":"main_file"}],"oa_version":"Published Version","date_created":"2025-12-28T23:01:27Z","pmid":1,"_id":"20859","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"keyword":["thermobiology","cell migration","thermo-adaptability of immune cells"],"date_updated":"2026-07-23T06:27:15Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","OA_place":"publisher","article_type":"original","acknowledgement":"The authors would like to acknowledge the Super Resolution Light Microcopy and Nanoscopy (SLN) Facility of ICFO for their support with imaging experiments, Johann Osmond (Nanofabrication laboratory, ICFO) for the design and production of molds for generating confinement coverslip, Merche Rivas for cell culture of immune cells and further support from the CRG Core Facilities for Genomics and Advanced Light Microscopy. We would like to thank Michael Sixt for discussions on this work and the Quidant, Ruprecht, and Wieser lab members for critical reading of the manuscript. This research was supported by the Scientific Service Units (SSU) of IST-Austria through resources provided by the Nanofabrication Facility (NFF). C.A. acknowledges the funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 847517 and V.V. from the ICFOstepstone – PhD Programme funded by the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no 665884. S.W. acknowledges support through the Spanish Ministry of Economy and Competitiveness via MINECO’s Plan Nacional (BFU2017-86296-P). V.R. acknowledges funding from the European Union’s HORIZON-EIC-2021-PATHFINDEROPEN program under grant agreement no. 101046620 and European Union's Horizon Europe program under the grant agreement no. 101072123. E.K. acknowledges funding by a fellowship of the Ministry of Innovation, Science and Research of North-Rhine-Westphalia (AZ: 421-8.03.03.02-137069) and the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy – EXC 2151 – 390873048 and by the TRA Life and Health (University of Bonn) as part of the Excellence Strategy of the federal and state governments.","department":[{"_id":"Bio"},{"_id":"NanoFab"}],"day":"11","das_tickbox":"1","author":[{"full_name":"Company-Garrido, Iván","last_name":"Company-Garrido","first_name":"Iván"},{"full_name":"Zurita Carpio, Alberto","first_name":"Alberto","last_name":"Zurita Carpio"},{"full_name":"Colomer-Rosell, Mariona","first_name":"Mariona","last_name":"Colomer-Rosell"},{"first_name":"Bernard","last_name":"Ciraulo","full_name":"Ciraulo, Bernard"},{"first_name":"Ronja","last_name":"Molkenbur","full_name":"Molkenbur, Ronja"},{"full_name":"Lanzerstorfer, Peter","first_name":"Peter","last_name":"Lanzerstorfer"},{"first_name":"Fabio","last_name":"Pezzano","full_name":"Pezzano, Fabio"},{"last_name":"Agazzi","first_name":"Costanza","full_name":"Agazzi, Costanza"},{"first_name":"Robert","last_name":"Hauschild","orcid":"0000-0001-9843-3522","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Jain","first_name":"Saumey","full_name":"Jain, Saumey"},{"full_name":"Jacques, Jeroen M.","last_name":"Jacques","first_name":"Jeroen M."},{"last_name":"Venturini","first_name":"Valeria","full_name":"Venturini, Valeria"},{"first_name":"Christian","last_name":"Knapp","full_name":"Knapp, Christian"},{"full_name":"Xie, Yufei","first_name":"Yufei","last_name":"Xie"},{"orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","first_name":"Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Weghuber, Julian","last_name":"Weghuber","first_name":"Julian"},{"full_name":"Schaaf, Marcel","last_name":"Schaaf","first_name":"Marcel"},{"last_name":"Quidant","first_name":"Romain","full_name":"Quidant, Romain"},{"id":"3EB04B78-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6165-5738","full_name":"Kiermaier, Eva","last_name":"Kiermaier","first_name":"Eva"},{"full_name":"Ortega Arroyo, Jaime","last_name":"Ortega Arroyo","first_name":"Jaime"},{"first_name":"Verena","last_name":"Ruprecht","full_name":"Ruprecht, Verena","orcid":"0000-0003-4088-8633","id":"4D71A03A-F248-11E8-B48F-1D18A9856A87"},{"id":"355AA5A0-F248-11E8-B48F-1D18A9856A87","first_name":"Stefan","last_name":"Wieser","full_name":"Wieser, Stefan","orcid":"0000-0002-2670-2217"}],"title":"Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses"},{"_id":"20636","pmid":1,"date_created":"2025-11-12T10:03:39Z","oa_version":"None","publication":"Developmental Cell","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"OA_type":"closed access","date_published":"2026-01-14T00:00:00Z","researchdata_availability":"upon request","external_id":{"pmid":["41043435"]},"author":[{"full_name":"Huang, R","first_name":"R","last_name":"Huang"},{"first_name":"J","last_name":"Wang","full_name":"Wang, J"},{"last_name":"Chang","first_name":"M","full_name":"Chang, M"},{"last_name":"Tang","first_name":"W","full_name":"Tang, W"},{"last_name":"Yu","first_name":"Y","full_name":"Yu, Y"},{"first_name":"Y","last_name":"Zhang","full_name":"Zhang, Y"},{"full_name":"Peng, Y","last_name":"Peng","first_name":"Y"},{"full_name":"Wang, Y","first_name":"Y","last_name":"Wang"},{"full_name":"Guo, Y","first_name":"Y","last_name":"Guo"},{"full_name":"Lu, T","first_name":"T","last_name":"Lu"},{"last_name":"Cao","first_name":"Y","full_name":"Cao, Y"},{"last_name":"Zhou","first_name":"Y","full_name":"Zhou, Y"},{"first_name":"Q","last_name":"Zhang","full_name":"Zhang, Q"},{"first_name":"Y","last_name":"Huang","full_name":"Huang, Y"},{"last_name":"Wu","first_name":"A","full_name":"Wu, A"},{"first_name":"L","last_name":"Ren","full_name":"Ren, L"},{"id":"35A03822-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","first_name":"Michelle C","last_name":"Gallei"},{"full_name":"Dong, J","first_name":"J","last_name":"Dong"},{"last_name":"Chen","first_name":"H","full_name":"Chen, H"},{"full_name":"He, J","first_name":"J","last_name":"He"},{"full_name":"Wen, M","first_name":"M","last_name":"Wen"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"full_name":"Sun, L","first_name":"L","last_name":"Sun"},{"first_name":"Y","last_name":"Xiong","full_name":"Xiong, Y"},{"first_name":"Z","last_name":"Yang","full_name":"Yang, Z"},{"full_name":"Xu, T","first_name":"T","last_name":"Xu"}],"title":"TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis","das_tickbox":"1","department":[{"_id":"JiFr"}],"day":"14","acknowledgement":"We thank Lukáš Fiedler‬ for helping with the writing. This work was supported by the National Key Research and Development Program of China (2023YFA0913500) to T.X., R.H., Y.Y., Y.X., and M.W. and by the National Natural Science Foundation of China grants to T.X. (32130010), Z.Y. (3241101698), and R.H. (32070309 and 32470276) and startup funds from the Fujian Agriculture and Forestry University and the Shanghai Plant Stress Biology Center, Chinese Academy of Sciences to T.X.","article_type":"original","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-07-27T08:02:04Z","status":"public","publisher":"Elsevier","type":"journal_article","supplementarymaterial":"yes","publication_status":"published","intvolume":"        61","dataavailabilitystatement":"Plasmids and genetic materials generated in this study will be made available upon request from the lead contact.The mass spectrometry data reported in this work have been deposited at the iProX database. Original western blot gel images have been deposited to Mendeley Data. The accession code and the DOI are listed in the key resources table.\r\nThis paper does not report original code. Any additional information required to reanalyze the data reported in this paper is available from the lead contact upon request.","doi":"10.1016/j.devcel.2025.09.009","scopus_import":"1","abstract":[{"text":"The versatile and pivotal roles of the phytohormone auxin in regulating plant growth and development are typically linked to its directional transport, relying on the polarized PIN-FORMED (PIN) auxin exporters at the plasma membrane (PM). For decades, auxin has been proposed to promote PIN polarization, generating self-regulatory feedback mediating much of plant development, but mechanistic insight into this regulation is lacking. Here, we uncover an auxin-induced protein complex at the PM, containing auxin co-receptors transmembrane kinases (TMKs) and PIN1 auxin exporter, as the core machinery that underlies this feedback regulation. Auxin promotes PIN1 phosphorylation by TMKs, modulating PIN1 polarization and transport activity. We also provide evidence that PIN1-exported extracellular auxin is crucial for TMK activation and cell elongation, thus forming the simplest two-element self-regulatory feedback circuit. Thus, these findings offer direct mechanistic insights into a potential self-organizing circuit for auxin signaling and transport to ensure proper plant development in Arabidopsis.","lang":"eng"}],"citation":{"apa":"Huang, R., Wang, J., Chang, M., Tang, W., Yu, Y., Zhang, Y., … Xu, T. (2026). TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>","mla":"Huang, R., et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>, vol. 61, no. 1, Elsevier, 2026, pp. 73–84, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>.","ama":"Huang R, Wang J, Chang M, et al. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. <i>Developmental Cell</i>. 2026;61(1):73-84. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">10.1016/j.devcel.2025.09.009</a>","short":"R. Huang, J. Wang, M. Chang, W. Tang, Y. Yu, Y. Zhang, Y. Peng, Y. Wang, Y. Guo, T. Lu, Y. Cao, Y. Zhou, Q. Zhang, Y. Huang, A. Wu, L. Ren, M.C. Gallei, J. Dong, H. Chen, J. He, M. Wen, J. Friml, L. Sun, Y. Xiong, Z. Yang, T. Xu, Developmental Cell 61 (2026) 73–84.","chicago":"Huang, R, J Wang, M Chang, W Tang, Y Yu, Y Zhang, Y Peng, et al. “TMK-PIN1 Drives a Short Self-Organizing Circuit for Auxin Export and Signaling in Arabidopsis.” <i>Developmental Cell</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.devcel.2025.09.009\">https://doi.org/10.1016/j.devcel.2025.09.009</a>.","ieee":"R. Huang <i>et al.</i>, “TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis,” <i>Developmental Cell</i>, vol. 61, no. 1. Elsevier, pp. 73–84, 2026.","ista":"Huang R, Wang J, Chang M, Tang W, Yu Y, Zhang Y, Peng Y, Wang Y, Guo Y, Lu T, Cao Y, Zhou Y, Zhang Q, Huang Y, Wu A, Ren L, Gallei MC, Dong J, Chen H, He J, Wen M, Friml J, Sun L, Xiong Y, Yang Z, Xu T. 2026. TMK-PIN1 drives a short self-organizing circuit for auxin export and signaling in Arabidopsis. Developmental Cell. 61(1), 73–84."},"article_processing_charge":"No","page":"73-84","issue":"1","month":"01","year":"2026","quality_controlled":"1","volume":61,"language":[{"iso":"eng"}]},{"external_id":{"pmid":["40262524"],"isi":["001477400800001"]},"date_published":"2025-04-21T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"OA_type":"closed access","publication":"Developmental Cell","date_created":"2025-04-20T22:01:28Z","oa_version":"None","_id":"19594","pmid":1,"status":"public","date_updated":"2025-09-30T12:07:36Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"letter_note","department":[{"_id":"EvBe"}],"day":"21","author":[{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva","last_name":"Benková","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"}],"title":"Unlocking plant regeneration: The role for glutathione","citation":{"apa":"Benková, E. (2025). Unlocking plant regeneration: The role for glutathione. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">https://doi.org/10.1016/j.devcel.2025.03.012</a>","mla":"Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” <i>Developmental Cell</i>, vol. 60, no. 8, Elsevier, 2025, pp. 1137–39, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">10.1016/j.devcel.2025.03.012</a>.","ieee":"E. Benková, “Unlocking plant regeneration: The role for glutathione,” <i>Developmental Cell</i>, vol. 60, no. 8. Elsevier, pp. 1137–1139, 2025.","ista":"Benková E. 2025. Unlocking plant regeneration: The role for glutathione. Developmental Cell. 60(8), 1137–1139.","chicago":"Benková, Eva. “Unlocking Plant Regeneration: The Role for Glutathione.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">https://doi.org/10.1016/j.devcel.2025.03.012</a>.","ama":"Benková E. Unlocking plant regeneration: The role for glutathione. <i>Developmental Cell</i>. 2025;60(8):1137-1139. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.03.012\">10.1016/j.devcel.2025.03.012</a>","short":"E. Benková, Developmental Cell 60 (2025) 1137–1139."},"abstract":[{"text":"In this issue of Developmental Cell, Lee et al. identify a pivotal role for glutathione (GSH) in plant regeneration, a vital biological process enabling plants to regrow tissues and organs after injury. Applying single-cell RNA sequencing (scRNA-seq) and live imaging, the authors demonstrate that GSH, released upon tissue damage, accelerates cell-cycle transitions, particularly shortening the G1 phase, thereby facilitating efficient organ regeneration.","lang":"eng"}],"scopus_import":"1","doi":"10.1016/j.devcel.2025.03.012","intvolume":"        60","publication_status":"published","corr_author":"1","publisher":"Elsevier","type":"journal_article","language":[{"iso":"eng"}],"volume":60,"quality_controlled":"1","year":"2025","month":"04","issue":"8","page":"1137-1139","article_processing_charge":"No","isi":1},{"publication":"Developmental Cell","file":[{"file_name":"2025_DevelopmentalCell_McLaren.pdf","creator":"dernst","date_created":"2025-12-29T13:45:05Z","checksum":"1ca6f0822c1cbd430686d5e2a4f96401","file_size":12564806,"relation":"main_file","access_level":"open_access","date_updated":"2025-12-29T13:45:05Z","success":1,"content_type":"application/pdf","file_id":"20872"}],"oa_version":"Published Version","date_created":"2025-05-18T22:02:50Z","pmid":1,"project":[{"name":"Design Principles of Branching Morphogenesis","call_identifier":"H2020","_id":"05943252-7A3F-11EA-A408-12923DDC885E","grant_number":"851288"}],"_id":"19703","external_id":{"isi":["001570502100005"],"pmid":["40347948"]},"oa":1,"date_published":"2025-09-08T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"OA_type":"hybrid","OA_place":"publisher","article_type":"original","acknowledgement":"We thank A. Dimitracopoulos, K. Kawaguchi, J. Vidigueira, B. Baum, I. McLaren, D. St Johnston, and members of the Buckley, Scarpa, Steventon, Kawaguchi, and Xiong labs for technical assistance and constructive feedback. We thank Ryan Greenhalgh for methods developed to obtain fluidity values from AFM data. We thank Nicola Lawrence, Alex Sossick, and Sargon Gross-Thebing from the Gurdon Institute Imaging Facility for microscopy support. Funding: this work was supported by a Wellcome Trust/Royal Society Sir Henry Dale Fellowship (215439/Z/19/Z) and UKRI-EPSRC Frontier Research Grant (EP/X023761/1, originally selected as an ERC Starting Grant) to F.X.; an ERC Consolidator Grant (772426), ERC Synergy Grant 101118729 UNFOLD, and Alexander von Humboldt Professorship ( Alexander von Humboldt Foundation) to K.F.; and an ERC Starting Grant (851288) to E.H.","day":"08","department":[{"_id":"EdHa"}],"ec_funded":1,"title":"Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord","author":[{"first_name":"Susannah B.P.","last_name":"Mclaren","full_name":"Mclaren, Susannah B.P."},{"full_name":"Xue, Shi-lei","last_name":"Xue","first_name":"Shi-lei","id":"31D2C804-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Ding","first_name":"Siyuan","full_name":"Ding, Siyuan"},{"first_name":"Alexander K.","last_name":"Winkel","full_name":"Winkel, Alexander K."},{"full_name":"Baldwin, Oscar","last_name":"Baldwin","first_name":"Oscar"},{"last_name":"Dwarakacherla","first_name":"Shreya","full_name":"Dwarakacherla, Shreya"},{"last_name":"Franze","first_name":"Kristian","full_name":"Franze, Kristian"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","first_name":"Edouard B","last_name":"Hannezo"},{"full_name":"Xiong, Fengzhu","last_name":"Xiong","first_name":"Fengzhu"}],"status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_updated":"2025-12-29T14:58:14Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","intvolume":"        60","PlanS_conform":"1","publication_status":"published","publisher":"Elsevier","type":"journal_article","citation":{"chicago":"Mclaren, Susannah B.P., Shi-lei Xue, Siyuan Ding, Alexander K. Winkel, Oscar Baldwin, Shreya Dwarakacherla, Kristian Franze, Edouard B Hannezo, and Fengzhu Xiong. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>.","ieee":"S. B. P. Mclaren <i>et al.</i>, “Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord,” <i>Developmental Cell</i>, vol. 60, no. 17. Elsevier, p. 2237–2247.e4, 2025.","ista":"Mclaren SBP, Xue S, Ding S, Winkel AK, Baldwin O, Dwarakacherla S, Franze K, Hannezo EB, Xiong F. 2025. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. Developmental Cell. 60(17), 2237–2247.e4.","ama":"Mclaren SBP, Xue S, Ding S, et al. Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. 2025;60(17):2237-2247.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>","short":"S.B.P. Mclaren, S. Xue, S. Ding, A.K. Winkel, O. Baldwin, S. Dwarakacherla, K. Franze, E.B. Hannezo, F. Xiong, Developmental Cell 60 (2025) 2237–2247.e4.","apa":"Mclaren, S. B. P., Xue, S., Ding, S., Winkel, A. K., Baldwin, O., Dwarakacherla, S., … Xiong, F. (2025). Differential tissue deformability underlies fluid pressure-driven shape divergence of the avian embryonic brain and spinal cord. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">https://doi.org/10.1016/j.devcel.2025.04.010</a>","mla":"Mclaren, Susannah B. P., et al. “Differential Tissue Deformability Underlies Fluid Pressure-Driven Shape Divergence of the Avian Embryonic Brain and Spinal Cord.” <i>Developmental Cell</i>, vol. 60, no. 17, Elsevier, 2025, p. 2237–2247.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2025.04.010\">10.1016/j.devcel.2025.04.010</a>."},"abstract":[{"text":"An enlarged brain underlies the complex central nervous system of vertebrates. The dramatic expansion of the brain that diverges its shape from the spinal cord follows neural tube closure during embryonic development. Here, we show that this differential deformation is encoded by a pre-pattern of tissue material properties in chicken embryos. Using magnetic droplets and atomic force microscopy, we demonstrate that the dorsal hindbrain is more fluid than the dorsal spinal cord, resulting in a thinning versus a resisting response to increasing lumen pressure, respectively. The dorsal hindbrain exhibits reduced apical actin and a disorganized laminin matrix consistent with tissue fluidization. Blocking the activity of neural-crest-associated matrix metalloproteinases inhibits hindbrain expansion. Transplanting dorsal hindbrain cells to the spinal cord can locally create an expanded brain-like morphology in some cases. Our findings raise questions in vertebrate head evolution and suggest a general role of mechanical pre-patterning in sculpting epithelial tubes.","lang":"eng"}],"scopus_import":"1","doi":"10.1016/j.devcel.2025.04.010","month":"09","issue":"17","page":"2237-2247.e4","file_date_updated":"2025-12-29T13:45:05Z","article_processing_charge":"Yes (in subscription journal)","isi":1,"language":[{"iso":"eng"}],"volume":60,"quality_controlled":"1","ddc":["570"],"year":"2025"},{"file":[{"content_type":"application/pdf","success":1,"date_updated":"2025-06-04T05:43:27Z","access_level":"open_access","file_id":"19790","creator":"dernst","file_name":"2025_DevelopmentalCell_Jaeger.pdf","relation":"main_file","file_size":11936258,"checksum":"a83a4cb58f5941096d3ad91ca0172594","date_created":"2025-06-04T05:43:27Z"}],"publication":"Developmental Cell","_id":"15016","project":[{"name":"Development of V1 interneuron diversity during swim-to-walk transition of Xenopus metamorphosis","grant_number":"FTI21-D-046","_id":"bd73af52-d553-11ed-ba76-912049f0ac7a"},{"name":"Development and Evolution of Tetrapod Motor Circuits","grant_number":"101041551","_id":"ebb66355-77a9-11ec-83b8-b8ac210a4dae"},{"name":"Stem Cell Modulation in Neural Development and Regeneration/ P14-Swim-to-limb transition: cell type to connection diversity","_id":"8da85f50-16d5-11f0-9cad-eab8b0ff6c9e","grant_number":"F7814"}],"pmid":1,"date_created":"2024-02-20T09:20:32Z","oa_version":"Published Version","oa":1,"external_id":{"pmid":["39603234"],"isi":["001444798600001"]},"OA_type":"hybrid","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"date_published":"2025-03-10T00:00:00Z","department":[{"_id":"LoSw"},{"_id":"MaDe"},{"_id":"GaNo"}],"day":"10","acknowledgement":"We thank members of the Sweeney, Tosches, Shein-Idelson, Yamaguchi, Kelley, and Cline Labs for their contributions to this project, discussion, and support. We additionally thank the Beckman Institute CLOVER Center and Viviana Gradinaru (Caltech), Kimberly Ritola (UNC NeuroTools), and Flavia Gomez-Leite (ISTA Viral Core) for AAV production and consultation; Andras Simon and Alberto Joven (Karolinska Institute) for feedback; Elizabeth Bagnato-Cohen (Columbia) for project coordination; our animal care and imaging facilities; the amphibian stock centers (NXR, EXRC, and XenopusExpress); and our funding sources: NSF IOS 2110086 (D.B.K., L.B.S., M.A.T., A.Y., and H.T.C.); US-Israel Binational Science Foundation (BSF) 2020702 (M.S.-I.); FTI Strategy Lower Austria Dissertation FT121-D-046 (D.V.); Horizon Europe ERC Starting Grant 101041551 and Special Research Programme (SFB) of the Austrian Science Fund (FWF) project F7814-B (L.B.S.); NIH grant R35GM146973, Rita Allen Foundation Award GA_032522_FE, and CZI Ben Barres Early Career Acceleration Award 2023-331758 (M.A.T.); EMBO Long-Term Fellowship ALTF 874-2021 (A.D.); and NSF GRFP DGE 2036197 (E.C.B.J.).","article_type":"original","OA_place":"publisher","author":[{"full_name":"Jaeger, Eliza C.B.","last_name":"Jaeger","first_name":"Eliza C.B."},{"last_name":"Vijatovic","first_name":"David","orcid":"0000-0002-5494-0941","full_name":"Vijatovic, David","id":"cf391e77-ec3c-11ea-a124-d69323410b58"},{"full_name":"Deryckere, Astrid","last_name":"Deryckere","first_name":"Astrid"},{"first_name":"Nikol","last_name":"Zorin","full_name":"Zorin, Nikol"},{"full_name":"Nguyen, Akemi L.","last_name":"Nguyen","first_name":"Akemi L."},{"id":"eaf2b366-cfd1-11ee-bbdf-c8790f800a05","full_name":"Ivanian, Georgiy","orcid":"0009-0002-3999-3735","first_name":"Georgiy","last_name":"Ivanian"},{"last_name":"Woych","first_name":"Jamie","full_name":"Woych, Jamie"},{"full_name":"Arnold, Rebecca C","first_name":"Rebecca C","last_name":"Arnold","id":"d6cce458-14c9-11ed-a755-c1c8fc6fde6f"},{"last_name":"Ortega Gurrola","first_name":"Alonso","full_name":"Ortega Gurrola, Alonso"},{"first_name":"Arik","last_name":"Shvartsman","full_name":"Shvartsman, Arik"},{"id":"a9492887-8972-11ed-ae7b-bfae10998254","last_name":"Barbieri","first_name":"Francesca","full_name":"Barbieri, Francesca"},{"full_name":"Toma, Florina-Alexandra","last_name":"Toma","first_name":"Florina-Alexandra","id":"85dd99f2-15b2-11ec-abd3-d1ae4d57f3b5"},{"full_name":"Gorbsky, Gary J.","first_name":"Gary J.","last_name":"Gorbsky"},{"full_name":"Horb, Marko E.","last_name":"Horb","first_name":"Marko E."},{"full_name":"Cline, Hollis T.","first_name":"Hollis T.","last_name":"Cline"},{"full_name":"Shay, Timothy F.","first_name":"Timothy F.","last_name":"Shay"},{"full_name":"Kelley, Darcy B.","first_name":"Darcy B.","last_name":"Kelley"},{"first_name":"Ayako","last_name":"Yamaguchi","full_name":"Yamaguchi, Ayako"},{"full_name":"Shein-Idelson, Mark","first_name":"Mark","last_name":"Shein-Idelson"},{"full_name":"Tosches, Maria Antonietta","first_name":"Maria Antonietta","last_name":"Tosches"},{"id":"56BE8254-C4F0-11E9-8E45-0B23E6697425","first_name":"Lora Beatrice Jaeger","last_name":"Sweeney","full_name":"Sweeney, Lora Beatrice Jaeger","orcid":"0000-0001-9242-5601"}],"title":"Adeno-associated viral tools to trace neural development and connectivity across amphibians","date_updated":"2026-08-12T13:57:57Z","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"status":"public","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","intvolume":"        60","has_accepted_license":"1","publisher":"Elsevier","corr_author":"1","type":"journal_article","publication_status":"published","abstract":[{"lang":"eng","text":"Amphibians, by virtue of their phylogenetic position, provide invaluable insights on nervous system evolution, development, and remodeling. The genetic toolkit for amphibians, however, remains limited. Recombinant adeno-associated viral vectors (AAVs) are a powerful alternative to transgenesis for labeling and manipulating neurons. Although successful in mammals, AAVs have never been shown to transduce amphibian cells efficiently. We screened AAVs in three amphibian species—the frogs Xenopus laevis and Pelophylax bedriagae and the salamander Pleurodeles waltl—and identified at least two AAV serotypes per species that transduce neurons. In developing amphibians, AAVs labeled groups of neurons generated at the same time during development. In the mature brain, AAVrg retrogradely traced long-range projections. Our study introduces AAVs as a tool for amphibian research, establishes a generalizable workflow for AAV screening in new species, and expands opportunities for cross-species comparisons of nervous system development, function, and evolution."}],"citation":{"ieee":"E. C. B. Jaeger <i>et al.</i>, “Adeno-associated viral tools to trace neural development and connectivity across amphibians,” <i>Developmental Cell</i>, vol. 60, no. 5. Elsevier, p. 794–812.e6, 2025.","ista":"Jaeger ECB, Vijatovic D, Deryckere A, Zorin N, Nguyen AL, Ivanian G, Woych J, Arnold RC, Ortega Gurrola A, Shvartsman A, Barbieri F, Toma F-A, Gorbsky GJ, Horb ME, Cline HT, Shay TF, Kelley DB, Yamaguchi A, Shein-Idelson M, Tosches MA, Sweeney LB. 2025. Adeno-associated viral tools to trace neural development and connectivity across amphibians. Developmental Cell. 60(5), 794–812.e6.","chicago":"Jaeger, Eliza C.B., David Vijatovic, Astrid Deryckere, Nikol Zorin, Akemi L. Nguyen, Georgiy Ivanian, Jamie Woych, et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>. Elsevier, 2025. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>.","short":"E.C.B. Jaeger, D. Vijatovic, A. Deryckere, N. Zorin, A.L. Nguyen, G. Ivanian, J. Woych, R.C. Arnold, A. Ortega Gurrola, A. Shvartsman, F. Barbieri, F.-A. Toma, G.J. Gorbsky, M.E. Horb, H.T. Cline, T.F. Shay, D.B. Kelley, A. Yamaguchi, M. Shein-Idelson, M.A. Tosches, L.B. Sweeney, Developmental Cell 60 (2025) 794–812.e6.","ama":"Jaeger ECB, Vijatovic D, Deryckere A, et al. Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. 2025;60(5):794-812.e6. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>","apa":"Jaeger, E. C. B., Vijatovic, D., Deryckere, A., Zorin, N., Nguyen, A. L., Ivanian, G., … Sweeney, L. B. (2025). Adeno-associated viral tools to trace neural development and connectivity across amphibians. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">https://doi.org/10.1016/j.devcel.2024.10.025</a>","mla":"Jaeger, Eliza C. B., et al. “Adeno-Associated Viral Tools to Trace Neural Development and Connectivity across Amphibians.” <i>Developmental Cell</i>, vol. 60, no. 5, Elsevier, 2025, p. 794–812.e6, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.10.025\">10.1016/j.devcel.2024.10.025</a>."},"doi":"10.1016/j.devcel.2024.10.025","related_material":{"record":[{"status":"for_moderation","id":"22667","relation":"dissertation_contains"}]},"scopus_import":"1","file_date_updated":"2025-06-04T05:43:27Z","page":"794-812.e6","issue":"5","month":"03","isi":1,"article_processing_charge":"Yes (via OA deal)","volume":60,"language":[{"iso":"eng"}],"ddc":["570"],"year":"2025","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"quality_controlled":"1"},{"title":"Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization","author":[{"last_name":"Hörmayer","first_name":"Lukas","full_name":"Hörmayer, Lukas","orcid":"0000-0001-8295-2926","id":"2EEE7A2A-F248-11E8-B48F-1D18A9856A87"},{"id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","first_name":"Juan C","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C"},{"full_name":"Trozzi, N","first_name":"N","last_name":"Trozzi"},{"id":"b52391fb-f636-11ee-939c-8a8c47552e8a","first_name":"Leonhard","last_name":"Spona","full_name":"Spona, Leonhard"},{"id":"2E46069C-F248-11E8-B48F-1D18A9856A87","first_name":"Saiko","last_name":"Yoshida","full_name":"Yoshida, Saiko"},{"id":"44E59624-F248-11E8-B48F-1D18A9856A87","first_name":"Petra","last_name":"Marhavá","full_name":"Marhavá, Petra"},{"first_name":"Silvia","last_name":"Caballero Mancebo","orcid":"0000-0002-5223-3346","full_name":"Caballero Mancebo, Silvia","id":"2F1E1758-F248-11E8-B48F-1D18A9856A87"},{"id":"38F4F166-F248-11E8-B48F-1D18A9856A87","last_name":"Benková","first_name":"Eva","orcid":"0000-0002-8510-9739","full_name":"Benková, Eva"},{"orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Y","last_name":"Dagdas","full_name":"Dagdas, Y"},{"first_name":"M","last_name":"Majda","full_name":"Majda, M"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"day":"20","department":[{"_id":"JiFr"},{"_id":"EvBe"},{"_id":"CaHe"}],"ec_funded":1,"article_type":"original","acknowledgement":"We are thankful to Simon Gilroy, Alexander Jones, and Lieven De Veylder for sharing published material. We thank the Imaging & Optics and Life Science Facilities at IST Austria, the Biooptics facility at GMI, and the Cellular Imaging Facility at DBMV UNIL for providing invaluable assistance. The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP7/2007-2013)/ERC grant agreement no. 742985, from the FWF under the stand-alone grant P29988, and from EMBO (ALTF 253-2023).","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_updated":"2025-09-04T13:32:08Z","status":"public","pmid":1,"project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"grant_number":"P29988","_id":"262EF96E-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"RNA-directed DNA methylation in plant development"}],"_id":"15301","oa_version":"Published Version","date_created":"2024-04-08T12:07:57Z","file":[{"access_level":"open_access","success":1,"content_type":"application/pdf","date_updated":"2024-08-20T11:22:16Z","file_id":"17452","file_name":"2024_DevelopmentalCell_Hoermayer.pdf","creator":"dernst","file_size":5195262,"checksum":"22b374fb50a40d380b7686c84258d271","date_created":"2024-08-20T11:22:16Z","relation":"main_file"}],"publication":"Developmental Cell","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"date_published":"2024-05-20T00:00:00Z","external_id":{"pmid":["38579717"],"isi":["001301584600001"]},"oa":1,"isi":1,"article_processing_charge":"Yes (via OA deal)","issue":"10","page":"1333-1344.e4","file_date_updated":"2024-08-20T11:22:16Z","month":"05","ddc":["570"],"year":"2024","quality_controlled":"1","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"volume":59,"language":[{"iso":"eng"}],"corr_author":"1","publisher":"Elsevier","type":"journal_article","publication_status":"published","intvolume":"        59","has_accepted_license":"1","related_material":{"link":[{"relation":"press_release","url":"https://ista.ac.at/en/news/how-plants-heal-wounds/","description":"News on ISTA website"}]},"doi":"10.1016/j.devcel.2024.03.009","scopus_import":"1","abstract":[{"lang":"eng","text":"Plant morphogenesis relies exclusively on oriented cell expansion and division. Nonetheless, the mechanism(s) determining division plane orientation remain elusive. Here, we studied tissue healing after laser-assisted wounding in roots of Arabidopsis thaliana and uncovered how mechanical forces stabilize and reorient the microtubule cytoskeleton for the orientation of cell division. We identified that root tissue functions as an interconnected cell matrix, with a radial gradient of tissue extendibility causing predictable tissue deformation after wounding. This deformation causes instant redirection of expansion in the surrounding cells and reorientation of microtubule arrays, ultimately predicting cell division orientation. Microtubules are destabilized under low tension, whereas stretching of cells, either through wounding or external aspiration, immediately induces their polymerization. The higher microtubule abundance in the stretched cell parts leads to the reorientation of microtubule arrays and, ultimately, informs cell division planes. This provides a long-sought mechanism for flexible re-arrangement of cell divisions by mechanical forces for tissue reconstruction and plant architecture."}],"citation":{"mla":"Hörmayer, Lukas, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>, vol. 59, no. 10, Elsevier, 2024, p. 1333–1344.e4, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>.","apa":"Hörmayer, L., Montesinos López, J. C., Trozzi, N., Spona, L., Yoshida, S., Marhavá, P., … Friml, J. (2024). Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>","ista":"Hörmayer L, Montesinos López JC, Trozzi N, Spona L, Yoshida S, Marhavá P, Caballero Mancebo S, Benková E, Heisenberg C-PJ, Dagdas Y, Majda M, Friml J. 2024. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. Developmental Cell. 59(10), 1333–1344.e4.","ieee":"L. Hörmayer <i>et al.</i>, “Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization,” <i>Developmental Cell</i>, vol. 59, no. 10. Elsevier, p. 1333–1344.e4, 2024.","chicago":"Hörmayer, Lukas, Juan C Montesinos López, N Trozzi, Leonhard Spona, Saiko Yoshida, Petra Marhavá, Silvia Caballero Mancebo, et al. “Mechanical Forces in Plant Tissue Matrix Orient Cell Divisions via Microtubule Stabilization.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">https://doi.org/10.1016/j.devcel.2024.03.009</a>.","ama":"Hörmayer L, Montesinos López JC, Trozzi N, et al. Mechanical forces in plant tissue matrix orient cell divisions via microtubule stabilization. <i>Developmental Cell</i>. 2024;59(10):1333-1344.e4. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.03.009\">10.1016/j.devcel.2024.03.009</a>","short":"L. Hörmayer, J.C. Montesinos López, N. Trozzi, L. Spona, S. Yoshida, P. Marhavá, S. Caballero Mancebo, E. Benková, C.-P.J. Heisenberg, Y. Dagdas, M. Majda, J. Friml, Developmental Cell 59 (2024) 1333–1344.e4."}},{"external_id":{"pmid":["39413780"],"isi":["001390774300001"]},"oa":1,"date_published":"2024-12-16T00:00:00Z","OA_type":"hybrid","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"publication":"Developmental Cell","file":[{"file_id":"18835","date_updated":"2025-01-13T09:20:15Z","content_type":"application/pdf","success":1,"access_level":"open_access","relation":"main_file","date_created":"2025-01-13T09:20:15Z","checksum":"34423ee9fb4e30334f3572eddf1da2ae","file_size":3675955,"creator":"dernst","file_name":"2024_DevelopmentalCell_Janacek.pdf"}],"oa_version":"Published Version","date_created":"2024-10-23T08:41:27Z","pmid":1,"_id":"18465","status":"public","tmp":{"short":"CC BY-NC (4.0)","name":"Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc/4.0/legalcode","image":"/images/cc_by_nc.png"},"date_updated":"2025-09-08T14:33:17Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","OA_place":"publisher","acknowledgement":"This work was funded by DFG3468/6-1, DFG3468/6-3, and SFB924 to U.Z.H. We thank Angela Alkofer and Helene Prunkl for excellent technical assistance and Xenopus maintenance. Christian Luschnig is acknowledged for sharing unpublished results and valuable discussions.","department":[{"_id":"JiFr"}],"day":"16","title":"Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport","author":[{"last_name":"Janacek","first_name":"DP","full_name":"Janacek, DP"},{"last_name":"Kolb","first_name":"M","full_name":"Kolb, M"},{"last_name":"Schulz","first_name":"L","full_name":"Schulz, L"},{"full_name":"Mergner, J","last_name":"Mergner","first_name":"J"},{"first_name":"B","last_name":"Kuster","full_name":"Kuster, B"},{"first_name":"Matous","last_name":"Glanc","orcid":"0000-0003-0619-7783","full_name":"Glanc, Matous","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"},{"first_name":"K","last_name":"Ten Tusscher","full_name":"Ten Tusscher, K"},{"full_name":"Schwechheimer, C","first_name":"C","last_name":"Schwechheimer"},{"full_name":"Hammes, UZ","last_name":"Hammes","first_name":"UZ"}],"citation":{"ista":"Janacek D, Kolb M, Schulz L, Mergner J, Kuster B, Glanc M, Friml J, Ten Tusscher K, Schwechheimer C, Hammes U. 2024. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. Developmental Cell. 59(14), S1534-5807(24)00569–0.","chicago":"Janacek, DP, M Kolb, L Schulz, J Mergner, B Kuster, Matous Glanc, Jiří Friml, K Ten Tusscher, C Schwechheimer, and UZ Hammes. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">https://doi.org/10.1016/j.devcel.2024.09.020</a>.","ieee":"D. Janacek <i>et al.</i>, “Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport,” <i>Developmental Cell</i>, vol. 59, no. 14. Elsevier, pp. S1534-5807(24)00569–0, 2024.","short":"D. Janacek, M. Kolb, L. Schulz, J. Mergner, B. Kuster, M. Glanc, J. Friml, K. Ten Tusscher, C. Schwechheimer, U. Hammes, Developmental Cell 59 (2024) S1534-5807(24)00569–0.","ama":"Janacek D, Kolb M, Schulz L, et al. Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. <i>Developmental Cell</i>. 2024;59(14):S1534-5807(24)00569-0. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">10.1016/j.devcel.2024.09.020</a>","mla":"Janacek, DP, et al. “Transport Properties of Canonical PIN-FORMED Proteins from Arabidopsis and the Role of the Loop Domain in Auxin Transport.” <i>Developmental Cell</i>, vol. 59, no. 14, Elsevier, 2024, pp. S1534-5807(24)00569-0, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">10.1016/j.devcel.2024.09.020</a>.","apa":"Janacek, D., Kolb, M., Schulz, L., Mergner, J., Kuster, B., Glanc, M., … Hammes, U. (2024). Transport properties of canonical PIN-FORMED proteins from Arabidopsis and the role of the loop domain in auxin transport. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.09.020\">https://doi.org/10.1016/j.devcel.2024.09.020</a>"},"abstract":[{"lang":"eng","text":"The phytohormone auxin is polarly transported in plants by PIN-FORMED (PIN) transporters and controls virtually all growth and developmental processes. Canonical PINs possess a long, largely disordered cytosolic loop. Auxin transport by canonical PINs is activated by loop phosphorylation by certain kinases. The structure of the PIN transmembrane domains was recently determined, their transport properties remained poorly characterized, and the role of the loop in the transport process was unclear. Here, we determined the quantitative kinetic parameters of auxin transport mediated by Arabidopsis PINs to mathematically model auxin distribution in roots and to test these predictions in vivo. Using chimeras between transmembrane and loop domains of different PINs, we demonstrate a strong correlation between transport parameters and physiological output, indicating that the loop domain is not only required to activate PIN-mediated auxin transport, but it has an additional role in the transport process by a currently unknown mechanism."}],"scopus_import":"1","doi":"10.1016/j.devcel.2024.09.020","has_accepted_license":"1","intvolume":"        59","publication_status":"published","publisher":"Elsevier","type":"journal_article","language":[{"iso":"eng"}],"volume":59,"quality_controlled":"1","ddc":["570"],"year":"2024","month":"12","issue":"14","page":"S1534-5807(24)00569-0","file_date_updated":"2025-01-13T09:20:15Z","article_processing_charge":"Yes (in subscription journal)","isi":1,"license":"https://creativecommons.org/licenses/by-nc/4.0/"},{"external_id":{"isi":["001289684800001"],"pmid":["38776925"]},"oa":1,"date_published":"2024-08-01T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"OA_type":"hybrid","publication":"Developmental Cell","file":[{"file_id":"18841","access_level":"open_access","date_updated":"2025-01-13T10:59:12Z","success":1,"content_type":"application/pdf","date_created":"2025-01-13T10:59:12Z","checksum":"fefdea9c02862b4bb74de49b65ce638a","file_size":6249076,"relation":"main_file","file_name":"2024_DevelopmentalCell_Krammer.pdf","creator":"dernst"}],"oa_version":"Published Version","date_created":"2024-06-16T22:01:07Z","pmid":1,"project":[{"grant_number":"101044579","_id":"bd7e737f-d553-11ed-ba76-d69ffb5ee3aa","name":"Mechanisms of tissue size regulation in spinal cord development"},{"name":"The regulatory logic of pattern formation in the vertebrate dorsal neural tube","_id":"9B9B39FA-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-011"}],"_id":"17148","status":"public","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"date_updated":"2026-08-12T22:30:36Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","article_type":"original","OA_place":"publisher","acknowledgement":"We thank P. Pasierbek, A.C. Moreno, T. Lendl, and K. Aumayr for microscopy support; G. Schmauss for FACS support; M. Novatchkova for assistance with Bioinformatic analyses; J. Ahel, A. Polikarpova, S. Horer, E. Cesare, and E. Norouzi for technical assistance; A. Meinhardt for supervision; DRESDEN-concept Genome Center, A. Vogt, A. Sommer, and the Vienna BioCenter NGS facility for RNA sequencing. We are grateful to M. Placzek and E. Martí for discussions about the floorplate; to S. Shvartsman for valuable input; to A. Aszodi, W. Masselink, and S. Raiders for advice on statistical analyses; to J. Cornwall Scoones, G. Martello, and Tanaka lab members for critical reading of the manuscript; E. Bassat and E. Chatzidaki for contributing schematics; and to K. Lust for support. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (grant agreement ERC AdG 742046) to E.M.T. This research was funded in whole or in part by the Austrian Science Fund (FWF) (10.55776/F7803-B) (Stem Cell Modulation) to E.M.T. and A.K., Sir Henry Wellcome postdoctoral fellowship to H.T.S., ELBE fellowship to K.I., and National Science Foundation (US) Phy 2013131 to E.S. The A.K. lab is also supported by ISTA and the European Research Council under Horizon Europe grant 101044579, and S.L. is supported by Gesellschaft für Forschungsförderung Niederösterreich m.b.H. fellowship SC19-011. This work was supported in part by the Francis Crick Institute, which receives its core funding from Cancer Research UK (CC001051), the UK Medical Research Council (CC001051), and the Wellcome Trust (CC001051). For the purpose of open access, the authors have applied a CC BY public copyright license to any author accepted manuscript (AAM) version arising from this submission.","day":"01","department":[{"_id":"AnKi"}],"author":[{"full_name":"Krammer, Teresa","last_name":"Krammer","first_name":"Teresa"},{"full_name":"Stuart, Hannah T.","first_name":"Hannah T.","last_name":"Stuart"},{"last_name":"Gromberg","first_name":"Elena","full_name":"Gromberg, Elena"},{"last_name":"Ishihara","first_name":"Keisuke","full_name":"Ishihara, Keisuke"},{"full_name":"Cislo, Dillon","first_name":"Dillon","last_name":"Cislo"},{"first_name":"Manuela","last_name":"Melchionda","full_name":"Melchionda, Manuela"},{"first_name":"Fernando","last_name":"Becerril Perez","full_name":"Becerril Perez, Fernando"},{"first_name":"Jingkui","last_name":"Wang","full_name":"Wang, Jingkui"},{"full_name":"Costantini, Elena","first_name":"Elena","last_name":"Costantini"},{"id":"4D9EC9B6-F248-11E8-B48F-1D18A9856A87","first_name":"Stefanie","last_name":"Rus","full_name":"Rus, Stefanie","orcid":"0000-0001-8703-1093"},{"last_name":"Arbanas","first_name":"Laura","full_name":"Arbanas, Laura"},{"full_name":"Hörmann, Alexandra","first_name":"Alexandra","last_name":"Hörmann"},{"full_name":"Neumüller, Ralph A.","last_name":"Neumüller","first_name":"Ralph A."},{"full_name":"Elvassore, Nicola","first_name":"Nicola","last_name":"Elvassore"},{"last_name":"Siggia","first_name":"Eric","full_name":"Siggia, Eric"},{"full_name":"Briscoe, James","last_name":"Briscoe","first_name":"James"},{"id":"3959A2A0-F248-11E8-B48F-1D18A9856A87","last_name":"Kicheva","first_name":"Anna","orcid":"0000-0003-4509-4998","full_name":"Kicheva, Anna"},{"last_name":"Tanaka","first_name":"Elly M.","full_name":"Tanaka, Elly M."}],"title":"Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition","citation":{"apa":"Krammer, T., Stuart, H. T., Gromberg, E., Ishihara, K., Cislo, D., Melchionda, M., … Tanaka, E. M. (2024). Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2024.04.021\">https://doi.org/10.1016/j.devcel.2024.04.021</a>","mla":"Krammer, Teresa, et al. “Mouse Neural Tube Organoids Self-Organize Floorplate through BMP-Mediated Cluster Competition.” <i>Developmental Cell</i>, vol. 59, no. 15, Elsevier, 2024, p. 1940–1953.e10, doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.04.021\">10.1016/j.devcel.2024.04.021</a>.","chicago":"Krammer, Teresa, Hannah T. Stuart, Elena Gromberg, Keisuke Ishihara, Dillon Cislo, Manuela Melchionda, Fernando Becerril Perez, et al. “Mouse Neural Tube Organoids Self-Organize Floorplate through BMP-Mediated Cluster Competition.” <i>Developmental Cell</i>. Elsevier, 2024. <a href=\"https://doi.org/10.1016/j.devcel.2024.04.021\">https://doi.org/10.1016/j.devcel.2024.04.021</a>.","ieee":"T. Krammer <i>et al.</i>, “Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition,” <i>Developmental Cell</i>, vol. 59, no. 15. Elsevier, p. 1940–1953.e10, 2024.","ista":"Krammer T, Stuart HT, Gromberg E, Ishihara K, Cislo D, Melchionda M, Becerril Perez F, Wang J, Costantini E, Rus S, Arbanas L, Hörmann A, Neumüller RA, Elvassore N, Siggia E, Briscoe J, Kicheva A, Tanaka EM. 2024. Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition. Developmental Cell. 59(15), 1940–1953.e10.","short":"T. Krammer, H.T. Stuart, E. Gromberg, K. Ishihara, D. Cislo, M. Melchionda, F. Becerril Perez, J. Wang, E. Costantini, S. Rus, L. Arbanas, A. Hörmann, R.A. Neumüller, N. Elvassore, E. Siggia, J. Briscoe, A. Kicheva, E.M. Tanaka, Developmental Cell 59 (2024) 1940–1953.e10.","ama":"Krammer T, Stuart HT, Gromberg E, et al. Mouse neural tube organoids self-organize floorplate through BMP-mediated cluster competition. <i>Developmental Cell</i>. 2024;59(15):1940-1953.e10. doi:<a href=\"https://doi.org/10.1016/j.devcel.2024.04.021\">10.1016/j.devcel.2024.04.021</a>"},"abstract":[{"lang":"eng","text":"During neural tube (NT) development, the notochord induces an organizer, the floorplate, which secretes Sonic Hedgehog (SHH) to pattern neural progenitors. Conversely, NT organoids (NTOs) from embryonic stem cells (ESCs) spontaneously form floorplates without the notochord, demonstrating that stem cells can self-organize without embryonic inducers. Here, we investigated floorplate self-organization in clonal mouse NTOs. Expression of the floorplate marker FOXA2 was initially spatially scattered before resolving into multiple clusters, which underwent competition and sorting, resulting in a stable “winning” floorplate. We identified that BMP signaling governed long-range cluster competition. FOXA2+ clusters expressed BMP4, suppressing FOXA2 in receiving cells while simultaneously expressing the BMP-inhibitor NOGGIN, promoting cluster persistence. Noggin mutation perturbed floorplate formation in NTOs and in the NT in vivo at mid/hindbrain regions, demonstrating how the floorplate can form autonomously without the notochord. Identifying the pathways governing organizer self-organization is critical for harnessing the developmental plasticity of stem cells in tissue engineering."}],"scopus_import":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"19763"}]},"doi":"10.1016/j.devcel.2024.04.021","has_accepted_license":"1","intvolume":"        59","publication_status":"published","type":"journal_article","publisher":"Elsevier","language":[{"iso":"eng"}],"volume":59,"quality_controlled":"1","ddc":["570"],"year":"2024","month":"08","page":"1940-1953.e10","issue":"15","file_date_updated":"2025-01-13T10:59:12Z","article_processing_charge":"Yes (in subscription journal)","isi":1,"license":"https://creativecommons.org/licenses/by-nc-nd/4.0/"},{"external_id":{"isi":["000982111800001"],"pmid":["36931269"]},"oa":1,"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"date_published":"2023-04-10T00:00:00Z","file":[{"checksum":"c80ca2ebc241232aacdb5aa4b4c80957","date_created":"2023-04-17T07:41:25Z","file_size":7925886,"relation":"main_file","file_name":"2023_DevelopmentalCell_Huljev.pdf","creator":"dernst","file_id":"12842","access_level":"open_access","date_updated":"2023-04-17T07:41:25Z","content_type":"application/pdf","success":1}],"publication":"Developmental Cell","project":[{"name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","call_identifier":"H2020","_id":"260F1432-B435-11E9-9278-68D0E5697425","grant_number":"742573"},{"_id":"26520D1E-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 850-2017","name":"Coordination of mesendoderm cell fate specification and internalization during zebrafish gastrulation"},{"name":"Coordination of mesendoderm fate specification and internalization during zebrafish gastrulation","grant_number":"LT000429","_id":"266BC5CE-B435-11E9-9278-68D0E5697425"}],"pmid":1,"_id":"12830","oa_version":"Published Version","date_created":"2023-04-16T22:01:07Z","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_updated":"2025-04-23T08:51:34Z","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","day":"10","department":[{"_id":"CaHe"},{"_id":"Bio"}],"ec_funded":1,"article_type":"original","acknowledgement":"We thank Andrea Pauli (IMP) and Edouard Hannezo (ISTA) for fruitful discussions and support with the SPIM experiments; the Heisenberg group, and especially Feyza Nur Arslan and Alexandra Schauer, for discussions and feedback; Michaela Jović (ISTA) for help with the quantitative real-time PCR protocol; the bioimaging and zebrafish facilities of ISTA for continuous support; Stephan Preibisch (Janelia Research Campus) for support with the SPIM data analysis; and Nobuhiro Nakamura (Tokyo Institute of Technology) for sharing α1-Na+/K+-ATPase antibody. This work was supported by funding from the European Union (European Research Council Advanced grant 742573 to C.-P.H.), postdoctoral fellowships from EMBO (LTF-850-2017) and HFSP (LT000429/2018-L2) to D.P., and a PhD fellowship from the Studienstiftung des deutschen Volkes to F.P.","author":[{"id":"44C6F6A6-F248-11E8-B48F-1D18A9856A87","full_name":"Huljev, Karla","last_name":"Huljev","first_name":"Karla"},{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","first_name":"Shayan","last_name":"Shamipour"},{"last_name":"Nunes Pinheiro","first_name":"Diana C","orcid":"0000-0003-4333-7503","full_name":"Nunes Pinheiro, Diana C","id":"2E839F16-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Preusser, Friedrich","last_name":"Preusser","first_name":"Friedrich"},{"id":"2705C766-9FE2-11EA-B224-C6773DDC885E","full_name":"Steccari, Irene","first_name":"Irene","last_name":"Steccari"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","first_name":"Christoph M","orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M"},{"first_name":"Suyash","last_name":"Naik","full_name":"Naik, Suyash","orcid":"0000-0001-8421-5508","id":"2C0B105C-F248-11E8-B48F-1D18A9856A87"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","full_name":"Heisenberg, Carl-Philipp J","first_name":"Carl-Philipp J","last_name":"Heisenberg"}],"title":"A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish","abstract":[{"lang":"eng","text":"Interstitial fluid (IF) accumulation between embryonic cells is thought to be important for embryo patterning and morphogenesis. Here, we identify a positive mechanical feedback loop between cell migration and IF relocalization and find that it promotes embryonic axis formation during zebrafish gastrulation. We show that anterior axial mesendoderm (prechordal plate [ppl]) cells, moving in between the yolk cell and deep cell tissue to extend the embryonic axis, compress the overlying deep cell layer, thereby causing IF to flow from the deep cell layer to the boundary between the yolk cell and the deep cell layer, directly ahead of the advancing ppl. This IF relocalization, in turn, facilitates ppl cell protrusion formation and migration by opening up the space into which the ppl moves and, thereby, the ability of the ppl to trigger IF relocalization by pushing against the overlying deep cell layer. Thus, embryonic axis formation relies on a hydraulic feedback loop between cell migration and IF relocalization."}],"citation":{"ama":"Huljev K, Shamipour S, Nunes Pinheiro DC, et al. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. <i>Developmental Cell</i>. 2023;58(7):582-596.e7. doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">10.1016/j.devcel.2023.02.016</a>","short":"K. Huljev, S. Shamipour, D.C. Nunes Pinheiro, F. Preusser, I. Steccari, C.M. Sommer, S. Naik, C.-P.J. Heisenberg, Developmental Cell 58 (2023) 582–596.e7.","ieee":"K. Huljev <i>et al.</i>, “A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish,” <i>Developmental Cell</i>, vol. 58, no. 7. Elsevier, p. 582–596.e7, 2023.","chicago":"Huljev, Karla, Shayan Shamipour, Diana C Nunes Pinheiro, Friedrich Preusser, Irene Steccari, Christoph M Sommer, Suyash Naik, and Carl-Philipp J Heisenberg. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">https://doi.org/10.1016/j.devcel.2023.02.016</a>.","ista":"Huljev K, Shamipour S, Nunes Pinheiro DC, Preusser F, Steccari I, Sommer CM, Naik S, Heisenberg C-PJ. 2023. A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. Developmental Cell. 58(7), 582–596.e7.","mla":"Huljev, Karla, et al. “A Hydraulic Feedback Loop between Mesendoderm Cell Migration and Interstitial Fluid Relocalization Promotes Embryonic Axis Formation in Zebrafish.” <i>Developmental Cell</i>, vol. 58, no. 7, Elsevier, 2023, p. 582–596.e7, doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">10.1016/j.devcel.2023.02.016</a>.","apa":"Huljev, K., Shamipour, S., Nunes Pinheiro, D. C., Preusser, F., Steccari, I., Sommer, C. M., … Heisenberg, C.-P. J. (2023). A hydraulic feedback loop between mesendoderm cell migration and interstitial fluid relocalization promotes embryonic axis formation in zebrafish. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2023.02.016\">https://doi.org/10.1016/j.devcel.2023.02.016</a>"},"doi":"10.1016/j.devcel.2023.02.016","scopus_import":"1","intvolume":"        58","has_accepted_license":"1","publisher":"Elsevier","type":"journal_article","corr_author":"1","publication_status":"published","volume":58,"language":[{"iso":"eng"}],"year":"2023","ddc":["570"],"quality_controlled":"1","acknowledged_ssus":[{"_id":"PreCl"},{"_id":"Bio"}],"page":"582-596.e7","issue":"7","file_date_updated":"2023-04-17T07:41:25Z","month":"04","isi":1,"article_processing_charge":"Yes (via OA deal)"},{"publication_status":"published","corr_author":"1","type":"journal_article","publisher":"Elsevier","has_accepted_license":"1","intvolume":"        58","scopus_import":"1","doi":"10.1016/j.devcel.2023.06.001","citation":{"mla":"Leonard, Thomas A., et al. “The Membrane Surface as a Platform That Organizes Cellular and Biochemical Processes.” <i>Developmental Cell</i>, vol. 58, no. 15, Elsevier, 2023, pp. 1315–32, doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.06.001\">10.1016/j.devcel.2023.06.001</a>.","apa":"Leonard, T. A., Loose, M., &#38; Martens, S. (2023). The membrane surface as a platform that organizes cellular and biochemical processes. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2023.06.001\">https://doi.org/10.1016/j.devcel.2023.06.001</a>","short":"T.A. Leonard, M. Loose, S. Martens, Developmental Cell 58 (2023) 1315–1332.","ama":"Leonard TA, Loose M, Martens S. The membrane surface as a platform that organizes cellular and biochemical processes. <i>Developmental Cell</i>. 2023;58(15):1315-1332. doi:<a href=\"https://doi.org/10.1016/j.devcel.2023.06.001\">10.1016/j.devcel.2023.06.001</a>","chicago":"Leonard, Thomas A., Martin Loose, and Sascha Martens. “The Membrane Surface as a Platform That Organizes Cellular and Biochemical Processes.” <i>Developmental Cell</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.devcel.2023.06.001\">https://doi.org/10.1016/j.devcel.2023.06.001</a>.","ieee":"T. A. Leonard, M. Loose, and S. Martens, “The membrane surface as a platform that organizes cellular and biochemical processes,” <i>Developmental Cell</i>, vol. 58, no. 15. Elsevier, pp. 1315–1332, 2023.","ista":"Leonard TA, Loose M, Martens S. 2023. The membrane surface as a platform that organizes cellular and biochemical processes. Developmental Cell. 58(15), 1315–1332."},"abstract":[{"lang":"eng","text":"Membranes are essential for life. They act as semi-permeable boundaries that define cells and organelles. In addition, their surfaces actively participate in biochemical reaction networks, where they confine proteins, align reaction partners, and directly control enzymatic activities. Membrane-localized reactions shape cellular membranes, define the identity of organelles, compartmentalize biochemical processes, and can even be the source of signaling gradients that originate at the plasma membrane and reach into the cytoplasm and nucleus. The membrane surface is, therefore, an essential platform upon which myriad cellular processes are scaffolded. In this review, we summarize our current understanding of the biophysics and biochemistry of membrane-localized reactions with particular focus on insights derived from reconstituted and cellular systems. We discuss how the interplay of cellular factors results in their self-organization, condensation, assembly, and activity, and the emergent properties derived from them."}],"article_processing_charge":"Yes (via OA deal)","isi":1,"month":"08","file_date_updated":"2023-08-14T07:57:55Z","page":"1315-1332","issue":"15","quality_controlled":"1","ddc":["570"],"year":"2023","language":[{"iso":"eng"}],"volume":58,"date_created":"2023-08-13T22:01:12Z","oa_version":"Published Version","_id":"14039","project":[{"_id":"fc38323b-9c52-11eb-aca3-ff8afb4a011d","grant_number":"P34607","name":"In vitro reconstitution of bacterial cell division"},{"name":"Synthetic and structural biology of Rab GTPase networks","_id":"bd6ae2ca-d553-11ed-ba76-a4aa239da5ee","grant_number":"101045340"}],"pmid":1,"publication":"Developmental Cell","file":[{"file_id":"14049","date_updated":"2023-08-14T07:57:55Z","content_type":"application/pdf","success":1,"access_level":"open_access","relation":"main_file","checksum":"d8c5dc97cd40c26da2ec98ae723ab368","date_created":"2023-08-14T07:57:55Z","file_size":3184217,"creator":"dernst","file_name":"2023_DevelopmentalCell_Leonard.pdf"}],"date_published":"2023-08-07T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"oa":1,"external_id":{"pmid":["37419118"],"isi":["001059110400001"]},"author":[{"last_name":"Leonard","first_name":"Thomas A.","full_name":"Leonard, Thomas A."},{"orcid":"0000-0001-7309-9724","full_name":"Loose, Martin","last_name":"Loose","first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Sascha","last_name":"Martens","full_name":"Martens, Sascha"}],"title":"The membrane surface as a platform that organizes cellular and biochemical processes","acknowledgement":"We acknowledge funding from the Austrian Science Fund (FWF F79, P32814-B, and P35061-B to S.M.; P34607-B to M.L.; and P30584-B and P33066-B to T.A.L.) and the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant agreement no. 101045340 to M.L.). We are grateful for comments on the manuscript by Justyna Sawa-Makarska, Verena Baumann, Marko Kojic, Philipp Radler, Ronja Reinhardt, and Sumire Antonioli.","article_type":"original","day":"07","department":[{"_id":"MaLo"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","date_updated":"2024-10-22T11:40:18Z","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"}},{"intvolume":"        57","publication_status":"published","publisher":"Elsevier","type":"journal_article","citation":{"apa":"Martin, E. T., Blatt, P., Ngyuen, E., Lahr, R., Selvam, S., Yoon, H. A. M., … Rangan, P. (2022). A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2022.03.005\">https://doi.org/10.1016/j.devcel.2022.03.005</a>","mla":"Martin, Elliot T., et al. “A Translation Control Module Coordinates Germline Stem Cell Differentiation with Ribosome Biogenesis during Drosophila Oogenesis.” <i>Developmental Cell</i>, vol. 57, no. 7, Elsevier, 2022, p. 883–900.e10, doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.03.005\">10.1016/j.devcel.2022.03.005</a>.","short":"E.T. Martin, P. Blatt, E. Ngyuen, R. Lahr, S. Selvam, H.A.M. Yoon, T. Pocchiari, S. Emtenani, D.E. Siekhaus, A. Berman, G. Fuchs, P. Rangan, Developmental Cell 57 (2022) 883–900.e10.","ama":"Martin ET, Blatt P, Ngyuen E, et al. A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. <i>Developmental Cell</i>. 2022;57(7):883-900.e10. doi:<a href=\"https://doi.org/10.1016/j.devcel.2022.03.005\">10.1016/j.devcel.2022.03.005</a>","ieee":"E. T. Martin <i>et al.</i>, “A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis,” <i>Developmental Cell</i>, vol. 57, no. 7. Elsevier, p. 883–900.e10, 2022.","ista":"Martin ET, Blatt P, Ngyuen E, Lahr R, Selvam S, Yoon HAM, Pocchiari T, Emtenani S, Siekhaus DE, Berman A, Fuchs G, Rangan P. 2022. A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis. Developmental Cell. 57(7), 883–900.e10.","chicago":"Martin, Elliot T., Patrick Blatt, Elaine Ngyuen, Roni Lahr, Sangeetha Selvam, Hyun Ah M. Yoon, Tyler Pocchiari, et al. “A Translation Control Module Coordinates Germline Stem Cell Differentiation with Ribosome Biogenesis during Drosophila Oogenesis.” <i>Developmental Cell</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.devcel.2022.03.005\">https://doi.org/10.1016/j.devcel.2022.03.005</a>."},"abstract":[{"text":"Ribosomal defects perturb stem cell differentiation, causing diseases called ribosomopathies. How ribosome levels control stem cell differentiation is not fully known. Here, we discovered three RNA helicases are required for ribosome biogenesis and for Drosophila oogenesis. Loss of these helicases, which we named Aramis, Athos and Porthos, lead to aberrant stabilization of p53, cell cycle arrest and stalled GSC differentiation. Unexpectedly, Aramis is required for efficient translation of a cohort of mRNAs containing a 5’-Terminal-Oligo-Pyrimidine (TOP)-motif, including mRNAs that encode ribosomal proteins and a conserved p53 inhibitor, Novel Nucleolar protein 1 (Non1). The TOP-motif co-regulates the translation of growth-related mRNAs in mammals. As in mammals, the La-related protein co-regulates the translation of TOP-motif containing RNAs during Drosophila oogenesis. Thus, a previously unappreciated TOP-motif in Drosophila responds to reduced ribosome biogenesis to co-regulate the translation of ribosomal proteins and a p53 repressor, thus coupling ribosome biogenesis to GSC differentiation.","lang":"eng"}],"scopus_import":"1","doi":"10.1016/j.devcel.2022.03.005","month":"04","page":"883-900.e10","issue":"7","article_processing_charge":"No","isi":1,"language":[{"iso":"eng"}],"volume":57,"quality_controlled":"1","year":"2022","publication":"Developmental Cell","date_created":"2022-02-01T13:15:05Z","oa_version":"Preprint","_id":"10714","project":[{"_id":"2536F660-B435-11E9-9278-68D0E5697425","grant_number":"334077","name":"Investigating the role of transporters in invasive migration through junctions","call_identifier":"FP7"},{"name":"The role of Drosophila TNF alpha in immune cell invasion","call_identifier":"FWF","grant_number":"P29638","_id":"253B6E48-B435-11E9-9278-68D0E5697425"}],"pmid":1,"oa":1,"external_id":{"pmid":["35413237"],"isi":["000789021800005"]},"date_published":"2022-04-11T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"acknowledgement":"We are grateful to all members of the Rangan and Fuchs labs for their discussion and comments on the manuscript. We also thanks Dr. Sammons, Dr. Marlow, Life Science Editors, for their thoughts and comments the manuscript Additionally, we thank the Bloomington Stock Center, the Vienna Drosophila Resource Center, the BDGP Gene Disruption Project, and Flybase for fly stocks, reagents, and other resources. P.R. is funded by the NIH/NIGMS (R01GM111779-06 and RO1GM135628-01), G.F. is funded by NSF MCB-2047629 and NIH RO3 AI144839, D.E.S. was funded by Marie Curie CIG 334077/IRTIM and the Austrian Science Fund (FWF) grant ASI_FWF01_P29638S, and A.B is funded by NIH R01GM116889 and American Cancer Society RSG-17-197-01-RMC.","article_type":"original","ec_funded":1,"day":"11","department":[{"_id":"DaSi"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1101/2021.04.04.438367"}],"title":"A translation control module coordinates germline stem cell differentiation with ribosome biogenesis during Drosophila oogenesis","author":[{"full_name":"Martin, Elliot T.","last_name":"Martin","first_name":"Elliot T."},{"first_name":"Patrick","last_name":"Blatt","full_name":"Blatt, Patrick"},{"first_name":"Elaine","last_name":"Ngyuen","full_name":"Ngyuen, Elaine"},{"first_name":"Roni","last_name":"Lahr","full_name":"Lahr, Roni"},{"full_name":"Selvam, Sangeetha","last_name":"Selvam","first_name":"Sangeetha"},{"full_name":"Yoon, Hyun Ah M.","last_name":"Yoon","first_name":"Hyun Ah M."},{"full_name":"Pocchiari, Tyler","first_name":"Tyler","last_name":"Pocchiari"},{"id":"49D32318-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6981-6938","full_name":"Emtenani, Shamsi","first_name":"Shamsi","last_name":"Emtenani"},{"id":"3D224B9E-F248-11E8-B48F-1D18A9856A87","last_name":"Siekhaus","first_name":"Daria E","full_name":"Siekhaus, Daria E","orcid":"0000-0001-8323-8353"},{"full_name":"Berman, Andrea","last_name":"Berman","first_name":"Andrea"},{"first_name":"Gabriele","last_name":"Fuchs","full_name":"Fuchs, Gabriele"},{"first_name":"Prashanth","last_name":"Rangan","full_name":"Rangan, Prashanth"}],"status":"public","date_updated":"2025-06-12T06:19:50Z","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"publication_status":"published","corr_author":"1","publisher":"Cell Press","type":"journal_article","intvolume":"        57","scopus_import":"1","related_material":{"record":[{"status":"public","relation":"dissertation_contains","id":"20149"},{"status":"public","relation":"dissertation_contains","id":"12726"},{"id":"14530","relation":"dissertation_contains","status":"public"},{"status":"public","relation":"dissertation_contains","id":"12401"}]},"doi":"10.1016/j.devcel.2021.11.024","citation":{"ama":"Gaertner F, Dos Reis Rodrigues P, de Vries I, et al. WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. <i>Developmental Cell</i>. 2022;57(1):47-62.e9. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">10.1016/j.devcel.2021.11.024</a>","short":"F. Gaertner, P. Dos Reis Rodrigues, I. de Vries, M. Hons, J. Aguilera, M. Riedl, A.F. Leithner, S. Tasciyan, A. Kopf, J. Merrin, V. Zheden, W. Kaufmann, R. Hauschild, M.K. Sixt, Developmental Cell 57 (2022) 47–62.e9.","ieee":"F. Gaertner <i>et al.</i>, “WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues,” <i>Developmental Cell</i>, vol. 57, no. 1. Cell Press, p. 47–62.e9, 2022.","ista":"Gaertner F, Dos Reis Rodrigues P, de Vries I, Hons M, Aguilera J, Riedl M, Leithner AF, Tasciyan S, Kopf A, Merrin J, Zheden V, Kaufmann W, Hauschild R, Sixt MK. 2022. WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. Developmental Cell. 57(1), 47–62.e9.","chicago":"Gaertner, Florian, Patricia Dos Reis Rodrigues, Ingrid de Vries, Miroslav Hons, Juan Aguilera, Michael Riedl, Alexander F Leithner, et al. “WASp Triggers Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental Cell</i>. Cell Press, 2022. <a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">https://doi.org/10.1016/j.devcel.2021.11.024</a>.","apa":"Gaertner, F., Dos Reis Rodrigues, P., de Vries, I., Hons, M., Aguilera, J., Riedl, M., … Sixt, M. K. (2022). WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues. <i>Developmental Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">https://doi.org/10.1016/j.devcel.2021.11.024</a>","mla":"Gaertner, Florian, et al. “WASp Triggers Mechanosensitive Actin Patches to Facilitate Immune Cell Migration in Dense Tissues.” <i>Developmental Cell</i>, vol. 57, no. 1, Cell Press, 2022, p. 47–62.e9, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.11.024\">10.1016/j.devcel.2021.11.024</a>."},"abstract":[{"text":"When crawling through the body, leukocytes often traverse tissues that are densely packed with extracellular matrix and other cells, and this raises the question: How do leukocytes overcome compressive mechanical loads? Here, we show that the actin cortex of leukocytes is mechanoresponsive and that this responsiveness requires neither force sensing via the nucleus nor adhesive interactions with a substrate. Upon global compression of the cell body as well as local indentation of the plasma membrane, Wiskott-Aldrich syndrome protein (WASp) assembles into dot-like structures, providing activation platforms for Arp2/3 nucleated actin patches. These patches locally push against the external load, which can be obstructing collagen fibers or other cells, and thereby create space to facilitate forward locomotion. We show in vitro and in vivo that this WASp function is rate limiting for ameboid leukocyte migration in dense but not in loose environments and is required for trafficking through diverse tissues such as skin and lymph nodes.","lang":"eng"}],"article_processing_charge":"No","isi":1,"month":"01","page":"47-62.e9","issue":"1","quality_controlled":"1","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"},{"_id":"EM-Fac"}],"ddc":["570"],"year":"2022","language":[{"iso":"eng"}],"volume":57,"oa_version":"Published Version","date_created":"2022-01-30T23:01:33Z","project":[{"name":"Mechanical Adaptation of Lamellipodial Actin Networks in Migrating Cells","call_identifier":"H2020","grant_number":"747687","_id":"260AA4E2-B435-11E9-9278-68D0E5697425"},{"grant_number":"724373","_id":"25FE9508-B435-11E9-9278-68D0E5697425","name":"Cellular Navigation Along Spatial Gradients","call_identifier":"H2020"}],"pmid":1,"_id":"10703","publication":"Developmental Cell","date_published":"2022-01-10T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"external_id":{"isi":["000768933800005"],"pmid":["34919802"]},"oa":1,"main_file_link":[{"open_access":"1","url":"https://www.sciencedirect.com/science/article/pii/S1534580721009497"}],"author":[{"last_name":"Gaertner","first_name":"Florian","full_name":"Gaertner, Florian"},{"id":"26E95904-5160-11E9-9C0B-C5B0DC97E90F","first_name":"Patricia","last_name":"Dos Reis Rodrigues","orcid":"0000-0003-1681-508X","full_name":"Dos Reis Rodrigues, Patricia"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"De Vries","full_name":"De Vries, Ingrid"},{"id":"4167FE56-F248-11E8-B48F-1D18A9856A87","first_name":"Miroslav","last_name":"Hons","full_name":"Hons, Miroslav","orcid":"0000-0002-6625-3348"},{"full_name":"Aguilera, Juan","last_name":"Aguilera","first_name":"Juan"},{"first_name":"Michael","last_name":"Riedl","orcid":"0000-0003-4844-6311","full_name":"Riedl, Michael","id":"3BE60946-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Leithner","first_name":"Alexander F","full_name":"Leithner, Alexander F","orcid":"0000-0002-1073-744X","id":"3B1B77E4-F248-11E8-B48F-1D18A9856A87"},{"id":"4323B49C-F248-11E8-B48F-1D18A9856A87","first_name":"Saren","last_name":"Tasciyan","full_name":"Tasciyan, Saren","orcid":"0000-0003-1671-393X"},{"full_name":"Kopf, Aglaja","orcid":"0000-0002-2187-6656","last_name":"Kopf","first_name":"Aglaja","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87"},{"id":"4515C308-F248-11E8-B48F-1D18A9856A87","last_name":"Merrin","first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack"},{"id":"39C5A68A-F248-11E8-B48F-1D18A9856A87","first_name":"Vanessa","last_name":"Zheden","orcid":"0000-0002-9438-4783","full_name":"Zheden, Vanessa"},{"last_name":"Kaufmann","first_name":"Walter","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315","id":"3F99E422-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Robert","last_name":"Hauschild","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"}],"title":"WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissues","article_type":"original","acknowledgement":"We thank N. Darwish-Miranda, F. Leite, F.P. Assen, and A. Eichner for advice and help with experiments. We thank J. Renkawitz, E. Kiermaier, A. Juanes Garcia, and M. Avellaneda for critical reading of the manuscript. We thank M. Driscoll for advice on fluorescent labeling of collagen gels. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by Molecular Biology Services/Lab Support Facility (LSF)/Bioimaging Facility/Electron Microscopy Facility. This work was funded by grants from the European Research Council ( CoG 724373 ) and the Austrian Science Foundation (FWF) to M.S. F.G. received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement no. 747687.","department":[{"_id":"MiSi"},{"_id":"EM-Fac"},{"_id":"NanoFab"},{"_id":"BjHo"}],"day":"10","ec_funded":1,"user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","status":"public","tmp":{"short":"CC BY-NC-ND (4.0)","image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)"},"date_updated":"2026-08-12T22:30:09Z"},{"intvolume":"        56","publication_status":"published","corr_author":"1","type":"journal_article","publisher":"Elsevier","citation":{"mla":"Gärtner, Florian R., and Michael K. Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>, vol. 56, no. 6, Elsevier, 2021, pp. 723–25, doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>.","apa":"Gärtner, F. R., &#38; Sixt, M. K. (2021). Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>","short":"F.R. Gärtner, M.K. Sixt, Developmental Cell 56 (2021) 723–725.","ama":"Gärtner FR, Sixt MK. Engaging the front wheels to drive through fibrous terrain. <i>Developmental Cell</i>. 2021;56(6):723-725. doi:<a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">10.1016/j.devcel.2021.03.002</a>","chicago":"Gärtner, Florian R, and Michael K Sixt. “Engaging the Front Wheels to Drive through Fibrous Terrain.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2021.03.002\">https://doi.org/10.1016/j.devcel.2021.03.002</a>.","ieee":"F. R. Gärtner and M. K. Sixt, “Engaging the front wheels to drive through fibrous terrain,” <i>Developmental Cell</i>, vol. 56, no. 6. Elsevier, pp. 723–725, 2021.","ista":"Gärtner FR, Sixt MK. 2021. Engaging the front wheels to drive through fibrous terrain. Developmental Cell. 56(6), 723–725."},"abstract":[{"lang":"eng","text":"In this issue of Developmental Cell, Doyle and colleagues identify periodic anterior contraction as a characteristic feature of fibroblasts and mesenchymal cancer cells embedded in 3D collagen gels. This contractile mechanism generates a matrix prestrain required for crawling in fibrous 3D environments."}],"scopus_import":"1","doi":"10.1016/j.devcel.2021.03.002","month":"03","page":"723-725","issue":"6","article_processing_charge":"No","isi":1,"language":[{"iso":"eng"}],"volume":56,"quality_controlled":"1","year":"2021","ddc":["570"],"publication":"Developmental Cell","date_created":"2021-03-28T22:01:41Z","oa_version":"Published Version","_id":"9294","pmid":1,"oa":1,"external_id":{"isi":["000631681200004"],"pmid":["33756118"]},"date_published":"2021-03-22T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"article_type":"original","day":"22","department":[{"_id":"MiSi"}],"author":[{"id":"397A88EE-F248-11E8-B48F-1D18A9856A87","first_name":"Florian R","last_name":"Gärtner","orcid":"0000-0001-6120-3723","full_name":"Gärtner, Florian R"},{"id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","last_name":"Sixt","full_name":"Sixt, Michael K","orcid":"0000-0002-6620-9179"}],"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2021.03.002","open_access":"1"}],"title":"Engaging the front wheels to drive through fibrous terrain","status":"public","date_updated":"2026-06-18T19:45:10Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"},{"abstract":[{"lang":"eng","text":"Cytoplasm is a gel-like crowded environment composed of various macromolecules, organelles, cytoskeletal networks, and cytosol. The structure of the cytoplasm is highly organized and heterogeneous due to the crowding of its constituents and their effective compartmentalization. In such an environment, the diffusive dynamics of the molecules are restricted, an effect that is further amplified by clustering and anchoring of molecules. Despite the crowded nature of the cytoplasm at the microscopic scale, large-scale reorganization of the cytoplasm is essential for important cellular functions, such as cell division and polarization. How such mesoscale reorganization of the cytoplasm is achieved, especially for large cells such as oocytes or syncytial tissues that can span hundreds of micrometers in size, is only beginning to be understood. In this review, we will discuss recent advances in elucidating the molecular, cellular, and biophysical mechanisms by which the cytoskeleton drives cytoplasmic reorganization across different scales, structures, and species."}],"citation":{"mla":"Shamipour, Shayan, et al. “Cytoplasm’s Got Moves.” <i>Developmental Cell</i>, vol. 56, no. 2, Elsevier, 2021, pp. P213-226, doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.12.002\">10.1016/j.devcel.2020.12.002</a>.","apa":"Shamipour, S., Caballero Mancebo, S., &#38; Heisenberg, C.-P. J. (2021). Cytoplasm’s got moves. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2020.12.002\">https://doi.org/10.1016/j.devcel.2020.12.002</a>","ista":"Shamipour S, Caballero Mancebo S, Heisenberg C-PJ. 2021. Cytoplasm’s got moves. Developmental Cell. 56(2), P213-226.","chicago":"Shamipour, Shayan, Silvia Caballero Mancebo, and Carl-Philipp J Heisenberg. “Cytoplasm’s Got Moves.” <i>Developmental Cell</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.devcel.2020.12.002\">https://doi.org/10.1016/j.devcel.2020.12.002</a>.","ieee":"S. Shamipour, S. Caballero Mancebo, and C.-P. J. Heisenberg, “Cytoplasm’s got moves,” <i>Developmental Cell</i>, vol. 56, no. 2. Elsevier, pp. P213-226, 2021.","ama":"Shamipour S, Caballero Mancebo S, Heisenberg C-PJ. Cytoplasm’s got moves. <i>Developmental Cell</i>. 2021;56(2):P213-226. doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.12.002\">10.1016/j.devcel.2020.12.002</a>","short":"S. Shamipour, S. Caballero Mancebo, C.-P.J. Heisenberg, Developmental Cell 56 (2021) P213-226."},"doi":"10.1016/j.devcel.2020.12.002","related_material":{"record":[{"id":"9623","relation":"dissertation_contains","status":"public"}]},"scopus_import":"1","intvolume":"        56","type":"journal_article","publisher":"Elsevier","corr_author":"1","publication_status":"published","volume":56,"language":[{"iso":"eng"}],"year":"2021","ddc":["570"],"quality_controlled":"1","page":"P213-226","issue":"2","month":"01","isi":1,"article_processing_charge":"No","oa":1,"external_id":{"pmid":["33321104"],"isi":["000613273900009"]},"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"date_published":"2021-01-25T00:00:00Z","publication":"Developmental Cell","_id":"9006","pmid":1,"date_created":"2021-01-17T23:01:10Z","oa_version":"Published Version","date_updated":"2026-08-12T22:30:26Z","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","department":[{"_id":"CaHe"}],"day":"25","acknowledgement":"We would like to thank Justine Renno for illustrations and Edouard Hannezo and members of the Heisenberg group for their comments on previous versions of the manuscript.","article_type":"original","author":[{"id":"40B34FE2-F248-11E8-B48F-1D18A9856A87","full_name":"Shamipour, Shayan","last_name":"Shamipour","first_name":"Shayan"},{"id":"2F1E1758-F248-11E8-B48F-1D18A9856A87","first_name":"Silvia","last_name":"Caballero Mancebo","orcid":"0000-0002-5223-3346","full_name":"Caballero Mancebo, Silvia"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2020.12.002","open_access":"1"}],"title":"Cytoplasm's got moves"},{"publication":"Developmental Cell","file":[{"file_id":"9086","content_type":"application/pdf","success":1,"date_updated":"2021-02-04T10:20:02Z","access_level":"open_access","relation":"main_file","file_size":6929686,"date_created":"2021-02-04T10:20:02Z","checksum":"88e1a031a61689165d19a19c2f16d795","creator":"dernst","file_name":"2020_DevelopmCell_Chaigne.pdf"}],"oa_version":"Published Version","date_created":"2020-10-18T22:01:37Z","pmid":1,"_id":"8672","external_id":{"pmid":["32979313"],"isi":["000582501100012"]},"oa":1,"date_published":"2020-10-26T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"article_type":"original","acknowledgement":"This work was supported by the Medical Research Council UK (MRC Program award MC_UU_12018/5 ), the European Research Council (starting grant 311637 -MorphoCorDiv and consolidator grant 820188 -NanoMechShape to E.K.P.), and the Leverhulme Trust (Leverhulme Prize in Biological Sciences to E.K.P.). K.J.C. acknowledges support from the Royal Society (Royal Society Research Fellowship). A.C. acknowledges support from EMBO ( ALTF 2015-563 ), the Wellcome Trust ( 201334/Z/16/Z ), and the Fondation Bettencourt-Schueller (Prix Jeune Chercheur, 2015).","day":"26","department":[{"_id":"EdHa"}],"author":[{"full_name":"Chaigne, Agathe","last_name":"Chaigne","first_name":"Agathe"},{"last_name":"Labouesse","first_name":"Céline","full_name":"Labouesse, Céline"},{"first_name":"Ian J.","last_name":"White","full_name":"White, Ian J."},{"full_name":"Agnew, Meghan","last_name":"Agnew","first_name":"Meghan"},{"last_name":"Hannezo","first_name":"Edouard B","orcid":"0000-0001-6005-1561","full_name":"Hannezo, Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Chalut","first_name":"Kevin J.","full_name":"Chalut, Kevin J."},{"last_name":"Paluch","first_name":"Ewa K.","full_name":"Paluch, Ewa K."}],"title":"Abscission couples cell division to embryonic stem cell fate","status":"public","tmp":{"short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_updated":"2025-07-10T11:57:15Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","has_accepted_license":"1","intvolume":"        55","publication_status":"published","type":"journal_article","publisher":"Elsevier","citation":{"ieee":"A. Chaigne <i>et al.</i>, “Abscission couples cell division to embryonic stem cell fate,” <i>Developmental Cell</i>, vol. 55, no. 2. Elsevier, pp. 195–208, 2020.","chicago":"Chaigne, Agathe, Céline Labouesse, Ian J. White, Meghan Agnew, Edouard B Hannezo, Kevin J. Chalut, and Ewa K. Paluch. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” <i>Developmental Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">https://doi.org/10.1016/j.devcel.2020.09.001</a>.","ista":"Chaigne A, Labouesse C, White IJ, Agnew M, Hannezo EB, Chalut KJ, Paluch EK. 2020. Abscission couples cell division to embryonic stem cell fate. Developmental Cell. 55(2), 195–208.","short":"A. Chaigne, C. Labouesse, I.J. White, M. Agnew, E.B. Hannezo, K.J. Chalut, E.K. Paluch, Developmental Cell 55 (2020) 195–208.","ama":"Chaigne A, Labouesse C, White IJ, et al. Abscission couples cell division to embryonic stem cell fate. <i>Developmental Cell</i>. 2020;55(2):195-208. doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">10.1016/j.devcel.2020.09.001</a>","apa":"Chaigne, A., Labouesse, C., White, I. J., Agnew, M., Hannezo, E. B., Chalut, K. J., &#38; Paluch, E. K. (2020). Abscission couples cell division to embryonic stem cell fate. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">https://doi.org/10.1016/j.devcel.2020.09.001</a>","mla":"Chaigne, Agathe, et al. “Abscission Couples Cell Division to Embryonic Stem Cell Fate.” <i>Developmental Cell</i>, vol. 55, no. 2, Elsevier, 2020, pp. 195–208, doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.09.001\">10.1016/j.devcel.2020.09.001</a>."},"abstract":[{"text":"Cell fate transitions are key to development and homeostasis. It is thus essential to understand the cellular mechanisms controlling fate transitions. Cell division has been implicated in fate decisions in many stem cell types, including neuronal and epithelial progenitors. In other stem cells, such as embryonic stem (ES) cells, the role of division remains unclear. Here, we show that exit from naive pluripotency in mouse ES cells generally occurs after a division. We further show that exit timing is strongly correlated between sister cells, which remain connected by cytoplasmic bridges long after division, and that bridge abscission progressively accelerates as cells exit naive pluripotency. Finally, interfering with abscission impairs naive pluripotency exit, and artificially inducing abscission accelerates it. Altogether, our data indicate that a switch in the division machinery leading to faster abscission regulates pluripotency exit. Our study identifies abscission as a key cellular process coupling cell division to fate transitions.","lang":"eng"}],"scopus_import":"1","doi":"10.1016/j.devcel.2020.09.001","month":"10","page":"195-208","issue":"2","file_date_updated":"2021-02-04T10:20:02Z","article_processing_charge":"No","isi":1,"language":[{"iso":"eng"}],"volume":55,"quality_controlled":"1","year":"2020","ddc":["570"]},{"acknowledged_ssus":[{"_id":"Bio"},{"_id":"NanoFab"}],"quality_controlled":"1","year":"2020","language":[{"iso":"eng"}],"volume":55,"article_processing_charge":"No","isi":1,"month":"12","issue":"6","page":"695-706","scopus_import":"1","doi":"10.1016/j.devcel.2020.10.016","related_material":{"link":[{"description":"News on IST Homepage","relation":"press_release","url":"https://ist.ac.at/en/news/relaxing-cell-divisions/"}]},"citation":{"mla":"Godard, Benoit G., et al. “Apical Relaxation during Mitotic Rounding Promotes Tension-Oriented Cell Division.” <i>Developmental Cell</i>, vol. 55, no. 6, Elsevier, 2020, pp. 695–706, doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.10.016\">10.1016/j.devcel.2020.10.016</a>.","apa":"Godard, B. G., Dumollard, R., Munro, E., Chenevert, J., Hebras, C., Mcdougall, A., &#38; Heisenberg, C.-P. J. (2020). Apical relaxation during mitotic rounding promotes tension-oriented cell division. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2020.10.016\">https://doi.org/10.1016/j.devcel.2020.10.016</a>","chicago":"Godard, Benoit G, Rémi Dumollard, Edwin Munro, Janet Chenevert, Céline Hebras, Alex Mcdougall, and Carl-Philipp J Heisenberg. “Apical Relaxation during Mitotic Rounding Promotes Tension-Oriented Cell Division.” <i>Developmental Cell</i>. Elsevier, 2020. <a href=\"https://doi.org/10.1016/j.devcel.2020.10.016\">https://doi.org/10.1016/j.devcel.2020.10.016</a>.","ieee":"B. G. Godard <i>et al.</i>, “Apical relaxation during mitotic rounding promotes tension-oriented cell division,” <i>Developmental Cell</i>, vol. 55, no. 6. Elsevier, pp. 695–706, 2020.","ista":"Godard BG, Dumollard R, Munro E, Chenevert J, Hebras C, Mcdougall A, Heisenberg C-PJ. 2020. Apical relaxation during mitotic rounding promotes tension-oriented cell division. Developmental Cell. 55(6), 695–706.","ama":"Godard BG, Dumollard R, Munro E, et al. Apical relaxation during mitotic rounding promotes tension-oriented cell division. <i>Developmental Cell</i>. 2020;55(6):695-706. doi:<a href=\"https://doi.org/10.1016/j.devcel.2020.10.016\">10.1016/j.devcel.2020.10.016</a>","short":"B.G. Godard, R. Dumollard, E. Munro, J. Chenevert, C. Hebras, A. Mcdougall, C.-P.J. Heisenberg, Developmental Cell 55 (2020) 695–706."},"abstract":[{"lang":"eng","text":"Global tissue tension anisotropy has been shown to trigger stereotypical cell division orientation by elongating mitotic cells along the main tension axis. Yet, how tissue tension elongates mitotic cells despite those cells undergoing mitotic rounding (MR) by globally upregulating cortical actomyosin tension remains unclear. We addressed this question by taking advantage of ascidian embryos, consisting of a small number of interphasic and mitotic blastomeres and displaying an invariant division pattern. We found that blastomeres undergo MR by locally relaxing cortical tension at their apex, thereby allowing extrinsic pulling forces from neighboring interphasic blastomeres to polarize their shape and thus division orientation. Consistently, interfering with extrinsic forces by reducing the contractility of interphasic blastomeres or disrupting the establishment of asynchronous mitotic domains leads to aberrant mitotic cell division orientations. Thus, apical relaxation during MR constitutes a key mechanism by which tissue tension anisotropy controls stereotypical cell division orientation."}],"publication_status":"published","corr_author":"1","publisher":"Elsevier","type":"journal_article","intvolume":"        55","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","date_updated":"2025-07-10T12:01:28Z","author":[{"id":"33280250-F248-11E8-B48F-1D18A9856A87","full_name":"Godard, Benoit G","last_name":"Godard","first_name":"Benoit G"},{"full_name":"Dumollard, Rémi","last_name":"Dumollard","first_name":"Rémi"},{"full_name":"Munro, Edwin","last_name":"Munro","first_name":"Edwin"},{"full_name":"Chenevert, Janet","first_name":"Janet","last_name":"Chenevert"},{"first_name":"Céline","last_name":"Hebras","full_name":"Hebras, Céline"},{"full_name":"Mcdougall, Alex","last_name":"Mcdougall","first_name":"Alex"},{"id":"39427864-F248-11E8-B48F-1D18A9856A87","first_name":"Carl-Philipp J","last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566"}],"title":"Apical relaxation during mitotic rounding promotes tension-oriented cell division","acknowledgement":"We thank members of the Heisenberg and McDougall groups for technical advice and discussion, Hitoyoshi Yasuo for sharing lab equipment, Lucas Leclère and Hitoyoshi Yasuo for their comments on a preliminary version of the manuscript, and Philippe Dru for the Rose plots. We are grateful to the Bioimaging and Nanofabrication facilities of IST Austria and the Imaging Platform (PIM) and animal facility (CRB) of Institut de la Mer de Villefranche (IMEV), which is supported by EMBRC-France, whose French state funds are managed by the ANR within the Investments of the Future program under reference ANR-10-INBS-0, for continuous support. This work was supported by a grant from the French Government funding agency Agence National de la Recherche (ANR “MorCell”: ANR-17-CE 13-002 8).","article_type":"original","day":"21","department":[{"_id":"CaHe"}],"date_published":"2020-12-21T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"external_id":{"isi":["000600665700008"],"pmid":["33207225"]},"date_created":"2020-12-20T23:01:19Z","oa_version":"None","_id":"8957","pmid":1,"publication":"Developmental Cell"},{"title":"Emergence of an Apical Epithelial Cell Surface In Vivo","author":[{"last_name":"Sedzinski","first_name":"Jakub","full_name":"Sedzinski, Jakub"},{"id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","last_name":"Hannezo","first_name":"Edouard B","full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561"},{"last_name":"Tu","first_name":"Fan","full_name":"Tu, Fan"},{"full_name":"Biro, Maté","last_name":"Biro","first_name":"Maté"},{"first_name":"John","last_name":"Wallingford","full_name":"Wallingford, John"}],"main_file_link":[{"url":"https://doi.org/10.1016/j.devcel.2015.12.013","open_access":"1"}],"day":"12","OA_place":"publisher","article_type":"original","acknowledgement":"We thank J. Bear, B. Goldstein, A. Ewald, and D. Soroldoni for critical reading. This work was funded by an EMBO Long Term Fellowship to J.S., a Research Fellowship from Trinity College, Cambridge and a Bettencourt-Schueller Foundation Young Researcher Prize to E.H., a Cancer Institute NSW Early Career Researcher fellowship (13/ECF/1–25) and a Cancer Australia/Cure Cancer Australia Foundation project grant (1070498) to M.B., and grants from the NHLBI (HL117164) and NIGMS (GM074104) to J.B.W. J.B.W. was an early career scientist of the Howard Hughes Medical Institute. This work was initiated at the New Quantitative Approaches to Morphogenesis Workshop at UCSB, which is funded in part by the National Science Foundation (PHY11-25915) and the NIGMS (GM067110-05).","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-05-20T09:14:43Z","status":"public","pmid":1,"_id":"932","oa_version":"Published Version","date_created":"2018-12-11T11:49:16Z","publist_id":"6510","extern":"1","publication":"Developmental Cell","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"OA_type":"free access","date_published":"2016-01-12T00:00:00Z","external_id":{"pmid":["26766441"]},"oa":1,"article_processing_charge":"No","issue":"1","page":"24 - 35","month":"01","year":"2016","quality_controlled":"1","volume":36,"language":[{"iso":"eng"}],"publisher":"Elsevier","type":"journal_article","publication_status":"published","intvolume":"        36","doi":"10.1016/j.devcel.2015.12.013","scopus_import":"1","abstract":[{"text":"Epithelial sheets are crucial components of all metazoan animals, enclosing organs and protecting the animal from its environment. Epithelial homeostasis poses unique challenges, as addition of new cells and loss of old cells must be achieved without disrupting the fluid-tight barrier and apicobasal polarity of the epithelium. Several studies have identified cell biological mechanisms underlying extrusion of cells from epithelia, but far less is known of the converse mechanism by which new cells are added. Here, we combine molecular, pharmacological, and laser-dissection experiments with theoretical modeling to characterize forces driving emergence of an apical surface as single nascent cells are added to a vertebrate epithelium in vivo. We find that this process involves the interplay between cell-autonomous actin-generated pushing forces in the emerging cell and mechanical properties of neighboring cells. Our findings define the forces driving this cell behavior, contributing to a more comprehensive understanding of epithelial homeostasis.","lang":"eng"}],"citation":{"short":"J. Sedzinski, E.B. Hannezo, F. Tu, M. Biro, J. Wallingford, Developmental Cell 36 (2016) 24–35.","ama":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. Emergence of an Apical Epithelial Cell Surface In Vivo. <i>Developmental Cell</i>. 2016;36(1):24-35. doi:<a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">10.1016/j.devcel.2015.12.013</a>","ieee":"J. Sedzinski, E. B. Hannezo, F. Tu, M. Biro, and J. Wallingford, “Emergence of an Apical Epithelial Cell Surface In Vivo,” <i>Developmental Cell</i>, vol. 36, no. 1. Elsevier, pp. 24–35, 2016.","ista":"Sedzinski J, Hannezo EB, Tu F, Biro M, Wallingford J. 2016. Emergence of an Apical Epithelial Cell Surface In Vivo. Developmental Cell. 36(1), 24–35.","chicago":"Sedzinski, Jakub, Edouard B Hannezo, Fan Tu, Maté Biro, and John Wallingford. “Emergence of an Apical Epithelial Cell Surface In Vivo.” <i>Developmental Cell</i>. Elsevier, 2016. <a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">https://doi.org/10.1016/j.devcel.2015.12.013</a>.","apa":"Sedzinski, J., Hannezo, E. B., Tu, F., Biro, M., &#38; Wallingford, J. (2016). Emergence of an Apical Epithelial Cell Surface In Vivo. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">https://doi.org/10.1016/j.devcel.2015.12.013</a>","mla":"Sedzinski, Jakub, et al. “Emergence of an Apical Epithelial Cell Surface In Vivo.” <i>Developmental Cell</i>, vol. 36, no. 1, Elsevier, 2016, pp. 24–35, doi:<a href=\"https://doi.org/10.1016/j.devcel.2015.12.013\">10.1016/j.devcel.2015.12.013</a>."}},{"quality_controlled":"1","year":"2014","language":[{"iso":"eng"}],"volume":29,"article_processing_charge":"No","month":"06","page":"P729-739","issue":"6","scopus_import":"1","doi":"10.1016/j.devcel.2014.04.025","citation":{"apa":"Hofbauer, H. F., Schopf, F. H., Schleifer, H., Knittelfelder, O. L., Pieber, B., Rechberger, G. N., … Kohlwein, S. D. (2014). Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">https://doi.org/10.1016/j.devcel.2014.04.025</a>","mla":"Hofbauer, Harald F., et al. “Regulation of Gene Expression through a Transcriptional Repressor That Senses Acyl-Chain Length in Membrane Phospholipids.” <i>Developmental Cell</i>, vol. 29, no. 6, Elsevier, 2014, pp. P729-739, doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">10.1016/j.devcel.2014.04.025</a>.","chicago":"Hofbauer, Harald F., Florian H. Schopf, Hannes Schleifer, Oskar L. Knittelfelder, Bartholomäus Pieber, Gerald N. Rechberger, Heimo Wolinski, et al. “Regulation of Gene Expression through a Transcriptional Repressor That Senses Acyl-Chain Length in Membrane Phospholipids.” <i>Developmental Cell</i>. Elsevier, 2014. <a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">https://doi.org/10.1016/j.devcel.2014.04.025</a>.","ista":"Hofbauer HF, Schopf FH, Schleifer H, Knittelfelder OL, Pieber B, Rechberger GN, Wolinski H, Gaspar ML, Kappe CO, Stadlmann J, Mechtler K, Zenz A, Lohner K, Tehlivets O, Henry SA, Kohlwein SD. 2014. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. Developmental Cell. 29(6), P729-739.","ieee":"H. F. Hofbauer <i>et al.</i>, “Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids,” <i>Developmental Cell</i>, vol. 29, no. 6. Elsevier, pp. P729-739, 2014.","ama":"Hofbauer HF, Schopf FH, Schleifer H, et al. Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids. <i>Developmental Cell</i>. 2014;29(6):P729-739. doi:<a href=\"https://doi.org/10.1016/j.devcel.2014.04.025\">10.1016/j.devcel.2014.04.025</a>","short":"H.F. Hofbauer, F.H. Schopf, H. Schleifer, O.L. Knittelfelder, B. Pieber, G.N. Rechberger, H. Wolinski, M.L. Gaspar, C.O. Kappe, J. Stadlmann, K. Mechtler, A. Zenz, K. Lohner, O. Tehlivets, S.A. Henry, S.D. Kohlwein, Developmental Cell 29 (2014) P729-739."},"abstract":[{"lang":"eng","text":"Membrane phospholipids typically contain fatty acids (FAs) of 16 and 18 carbon atoms. This particular chain length is evolutionarily highly conserved and presumably provides maximum stability and dynamic properties to biological membranes in response to nutritional or environmental cues. Here, we show that the relative proportion of C16 versus C18 FAs is regulated by the activity of acetyl-CoA carboxylase (Acc1), the first and rate-limiting enzyme of FA de novo synthesis. Acc1 activity is attenuated by AMPK/Snf1-dependent phosphorylation, which is required to maintain an appropriate acyl-chain length distribution. Moreover, we find that the transcriptional repressor Opi1 preferentially binds to C16 over C18 phosphatidic acid (PA) species: thus, C16-chain containing PA sequesters Opi1 more effectively to the ER, enabling AMPK/Snf1 control of PA acyl-chain length to determine the degree of derepression of Opi1 target genes. These findings reveal an unexpected regulatory link between the major energy-sensing kinase, membrane lipid composition, and transcription."}],"publication_status":"published","type":"journal_article","publisher":"Elsevier","intvolume":"        29","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","status":"public","date_updated":"2023-02-21T10:09:45Z","title":"Regulation of gene expression through a transcriptional repressor that senses acyl-chain length in membrane phospholipids","author":[{"last_name":"Hofbauer","first_name":"Harald F.","full_name":"Hofbauer, Harald F."},{"full_name":"Schopf, Florian H.","first_name":"Florian H.","last_name":"Schopf"},{"full_name":"Schleifer, Hannes","first_name":"Hannes","last_name":"Schleifer"},{"first_name":"Oskar L.","last_name":"Knittelfelder","full_name":"Knittelfelder, Oskar L."},{"orcid":"0000-0001-8689-388X","full_name":"Pieber, Bartholomäus","first_name":"Bartholomäus","last_name":"Pieber","id":"93e5e5b2-0da6-11ed-8a41-af589a024726"},{"full_name":"Rechberger, Gerald N.","last_name":"Rechberger","first_name":"Gerald N."},{"first_name":"Heimo","last_name":"Wolinski","full_name":"Wolinski, Heimo"},{"first_name":"Maria L.","last_name":"Gaspar","full_name":"Gaspar, Maria L."},{"last_name":"Kappe","first_name":"C. Oliver","full_name":"Kappe, C. Oliver"},{"full_name":"Stadlmann, Johannes","last_name":"Stadlmann","first_name":"Johannes"},{"full_name":"Mechtler, Karl","first_name":"Karl","last_name":"Mechtler"},{"last_name":"Zenz","first_name":"Alexandra","full_name":"Zenz, Alexandra"},{"last_name":"Lohner","first_name":"Karl","full_name":"Lohner, Karl"},{"first_name":"Oksana","last_name":"Tehlivets","full_name":"Tehlivets, Oksana"},{"full_name":"Henry, Susan A.","first_name":"Susan A.","last_name":"Henry"},{"full_name":"Kohlwein, Sepp D.","first_name":"Sepp D.","last_name":"Kohlwein"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2014.04.025"}],"article_type":"original","day":"23","date_published":"2014-06-23T00:00:00Z","publication_identifier":{"eissn":["1878-1551"],"issn":["1534-5807"]},"external_id":{"pmid":["24960695"]},"oa":1,"oa_version":"Published Version","date_created":"2022-08-25T08:42:42Z","pmid":1,"_id":"11968","publication":"Developmental Cell","extern":"1"},{"day":"11","department":[{"_id":"DaZi"},{"_id":"XiFe"}],"article_type":"review","title":"A conversation across generations: Soma-germ cell crosstalk in plants","author":[{"id":"e0164712-22ee-11ed-b12a-d80fcdf35958","full_name":"Feng, Xiaoqi","orcid":"0000-0002-4008-1234","last_name":"Feng","first_name":"Xiaoqi"},{"orcid":"0000-0002-0123-8649","full_name":"Zilberman, Daniel","first_name":"Daniel","last_name":"Zilberman","id":"6973db13-dd5f-11ea-814e-b3e5455e9ed1"},{"full_name":"Dickinson, Hugh","last_name":"Dickinson","first_name":"Hugh"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2013.01.014"}],"date_updated":"2023-05-08T11:00:59Z","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","extern":"1","publication":"Developmental Cell","_id":"9520","pmid":1,"date_created":"2021-06-08T06:14:50Z","oa_version":"Published Version","oa":1,"external_id":{"pmid":["23410937"]},"publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"date_published":"2013-02-11T00:00:00Z","page":"215-225","issue":"3","month":"02","article_processing_charge":"No","volume":24,"language":[{"iso":"eng"}],"year":"2013","quality_controlled":"1","intvolume":"        24","type":"journal_article","publisher":"Elsevier","publication_status":"published","abstract":[{"lang":"eng","text":"Plants undergo alternation of generation in which reproductive cells develop in the plant body (\"sporophytic generation\") and then differentiate into a multicellular gamete-forming \"gametophytic generation.\" Different populations of helper cells assist in this transgenerational journey, with somatic tissues supporting early development and single nurse cells supporting gametogenesis. New data reveal a two-way relationship between early reproductive cells and their helpers involving complex epigenetic and signaling networks determining cell number and fate. Later, the egg cell plays a central role in specifying accessory cells, whereas in both gametophytes, companion cells contribute non-cell-autonomously to the epigenetic landscape of the gamete genomes."}],"citation":{"ista":"Feng X, Zilberman D, Dickinson H. 2013. A conversation across generations: Soma-germ cell crosstalk in plants. Developmental Cell. 24(3), 215–225.","chicago":"Feng, Xiaoqi, Daniel Zilberman, and Hugh Dickinson. “A Conversation across Generations: Soma-Germ Cell Crosstalk in Plants.” <i>Developmental Cell</i>. Elsevier, 2013. <a href=\"https://doi.org/10.1016/j.devcel.2013.01.014\">https://doi.org/10.1016/j.devcel.2013.01.014</a>.","ieee":"X. Feng, D. Zilberman, and H. Dickinson, “A conversation across generations: Soma-germ cell crosstalk in plants,” <i>Developmental Cell</i>, vol. 24, no. 3. Elsevier, pp. 215–225, 2013.","short":"X. Feng, D. Zilberman, H. Dickinson, Developmental Cell 24 (2013) 215–225.","ama":"Feng X, Zilberman D, Dickinson H. A conversation across generations: Soma-germ cell crosstalk in plants. <i>Developmental Cell</i>. 2013;24(3):215-225. doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.01.014\">10.1016/j.devcel.2013.01.014</a>","mla":"Feng, Xiaoqi, et al. “A Conversation across Generations: Soma-Germ Cell Crosstalk in Plants.” <i>Developmental Cell</i>, vol. 24, no. 3, Elsevier, 2013, pp. 215–25, doi:<a href=\"https://doi.org/10.1016/j.devcel.2013.01.014\">10.1016/j.devcel.2013.01.014</a>.","apa":"Feng, X., Zilberman, D., &#38; Dickinson, H. (2013). A conversation across generations: Soma-germ cell crosstalk in plants. <i>Developmental Cell</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2013.01.014\">https://doi.org/10.1016/j.devcel.2013.01.014</a>"},"doi":"10.1016/j.devcel.2013.01.014","scopus_import":"1"},{"publication_status":"published","type":"other_academic_publication","publisher":"Elsevier","intvolume":"        20","doi":"10.1016/j.devcel.2011.05.018","citation":{"ista":"Zilberman D. 2011. Balancing parental contributions in plant embryonic gene activation, Elsevier,p.","chicago":"Zilberman, Daniel. <i>Balancing Parental Contributions in Plant Embryonic Gene Activation</i>. <i>Developmental Cell</i>. Vol. 20. Elsevier, 2011. <a href=\"https://doi.org/10.1016/j.devcel.2011.05.018\">https://doi.org/10.1016/j.devcel.2011.05.018</a>.","ieee":"D. Zilberman, <i>Balancing parental contributions in plant embryonic gene activation</i>, vol. 20, no. 6. Elsevier, 2011, pp. 735–736.","short":"D. Zilberman, Balancing Parental Contributions in Plant Embryonic Gene Activation, Elsevier, 2011.","ama":"Zilberman D. <i>Balancing Parental Contributions in Plant Embryonic Gene Activation</i>. Vol 20. Elsevier; 2011:735-736. doi:<a href=\"https://doi.org/10.1016/j.devcel.2011.05.018\">10.1016/j.devcel.2011.05.018</a>","mla":"Zilberman, Daniel. “Balancing Parental Contributions in Plant Embryonic Gene Activation.” <i>Developmental Cell</i>, vol. 20, no. 6, Elsevier, 2011, pp. 735–36, doi:<a href=\"https://doi.org/10.1016/j.devcel.2011.05.018\">10.1016/j.devcel.2011.05.018</a>.","apa":"Zilberman, D. (2011). <i>Balancing parental contributions in plant embryonic gene activation</i>. <i>Developmental Cell</i> (Vol. 20, pp. 735–736). Elsevier. <a href=\"https://doi.org/10.1016/j.devcel.2011.05.018\">https://doi.org/10.1016/j.devcel.2011.05.018</a>"},"abstract":[{"text":"Little is known about chromatin remodeling events immediately after fertilization. A recent report by Autran et al. (2011) in Cell now shows that chromatin regulatory pathways that silence transposable elements are responsible for global delayed activation of gene expression in the early Arabidopsis embryo.","lang":"eng"}],"article_processing_charge":"No","month":"06","issue":"6","page":"735-736","quality_controlled":"1","year":"2011","language":[{"iso":"eng"}],"volume":20,"date_created":"2021-06-08T06:23:39Z","oa_version":"Published Version","_id":"9522","pmid":1,"publication":"Developmental Cell","extern":"1","date_published":"2011-06-14T00:00:00Z","publication_identifier":{"issn":["1534-5807"],"eissn":["1878-1551"]},"oa":1,"external_id":{"pmid":["21664571"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1016/j.devcel.2011.05.018"}],"author":[{"id":"6973db13-dd5f-11ea-814e-b3e5455e9ed1","orcid":"0000-0002-0123-8649","full_name":"Zilberman, Daniel","last_name":"Zilberman","first_name":"Daniel"}],"title":"Balancing parental contributions in plant embryonic gene activation","department":[{"_id":"DaZi"}],"day":"14","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","status":"public","date_updated":"2021-12-14T08:34:37Z"}]