---
OA_type: closed access
_id: '3945'
abstract:
- lang: eng
  text: Langerhans cells and dermal dendritic cells migrate to the draining lymph
    nodes through dermal lymphatic vessels. They do so in the steady-state and under
    inflammatory conditions. Peripheral T cell tolerance or T cell priming, respectively,
    are the consequences of migration. The nature of dendritic cell-containing vessels
    was mostly defined by electron microscopy or by their lack of blood endothelial
    markers. Selective markers for murine lymph endothelium were hitherto rare or
    not available. Here, we utilised recently developed antibodies against the murine
    hyaluronan receptor, LYVE-1, to study the lymph vessel network in mouse skin in
    more detail. In hairless skin from the ears, lymph vessels were spread out in
    a horizontal plane. They formed anastomoses, and they possessed frequent blind
    endings that were occasionally open. Lymph vessels were wider than blood vessels,
    which were identified by their strong CD31 expression. In body wall skin LYVE-1
    reactive vessels did not extend laterally but they dived straight down into the
    deeper dermis. There, they are connected to each other and formed a network similar
    to ear skin. The number and width of lymph vessels did not grossly change upon
    inflammatory stimuli such as skin explant culture or tape stripping. There were
    also no marked changes in caliber in response to the TLR 7/8 ligand Imiquimod.
    Double-labelling experiments of cultured skin showed that most of the strongly
    cell surface MHC II-expressing (i.e. activated) dendritic cells were confined
    to the lymph vessels. Langerin/CD207(+) cells within this population appeared
    later than dermal dendritic cells, i.e. langerin-negative cells. Comparable results
    were obtained after stimulating the skin in vivo with the TLR 7/8 ligand Imiquimod
    or by tape stripping. In untreated skin (i.e. steady state) a few MHC II(+) and
    Langerin/CD207(+) cells, presumably migrating skin dendritic cells including epidermal
    Langerhans cells, were consistently observed within the lymph vessels. The novel
    antibody reagents may serve as important tools to further study the dendritic
    cell traffic in the skin under physiological conditions as well as in conditions
    of adoptive dendritic cell transfer in immunotherapy.
article_processing_charge: No
article_type: original
author:
- first_name: Christoph
  full_name: Tripp, Christoph
  last_name: Tripp
- first_name: Bernhard
  full_name: Haid, Bernhard
  last_name: Haid
- first_name: Vincent
  full_name: Flacher, Vincent
  last_name: Flacher
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Hannes
  full_name: Peter, Hannes
  last_name: Peter
- first_name: Julia
  full_name: Farkas, Julia
  last_name: Farkas
- first_name: Robert
  full_name: Gschwentner, Robert
  last_name: Gschwentner
- first_name: Lydia
  full_name: Sorokin, Lydia
  last_name: Sorokin
- first_name: Nikolaus
  full_name: Romani, Nikolaus
  last_name: Romani
- first_name: Patrizia
  full_name: Stoitzner, Patrizia
  last_name: Stoitzner
citation:
  ama: Tripp C, Haid B, Flacher V, et al. The lymph vessel network in mouse skin visualised
    with antibodies against the hyaluronan receptor LYVE-1. <i>Immunobiology</i>.
    2008;213(9-10):715-728. doi:<a href="https://doi.org/10.1016/j.imbio.2008.07.025">10.1016/j.imbio.2008.07.025</a>
  apa: Tripp, C., Haid, B., Flacher, V., Sixt, M. K., Peter, H., Farkas, J., … Stoitzner,
    P. (2008). The lymph vessel network in mouse skin visualised with antibodies against
    the hyaluronan receptor LYVE-1. <i>Immunobiology</i>. Elsevier. <a href="https://doi.org/10.1016/j.imbio.2008.07.025">https://doi.org/10.1016/j.imbio.2008.07.025</a>
  chicago: Tripp, Christoph, Bernhard Haid, Vincent Flacher, Michael K Sixt, Hannes
    Peter, Julia Farkas, Robert Gschwentner, Lydia Sorokin, Nikolaus Romani, and Patrizia
    Stoitzner. “The Lymph Vessel Network in Mouse Skin Visualised with Antibodies
    against the Hyaluronan Receptor LYVE-1.” <i>Immunobiology</i>. Elsevier, 2008.
    <a href="https://doi.org/10.1016/j.imbio.2008.07.025">https://doi.org/10.1016/j.imbio.2008.07.025</a>.
  ieee: C. Tripp <i>et al.</i>, “The lymph vessel network in mouse skin visualised
    with antibodies against the hyaluronan receptor LYVE-1,” <i>Immunobiology</i>,
    vol. 213, no. 9–10. Elsevier, pp. 715–728, 2008.
  ista: Tripp C, Haid B, Flacher V, Sixt MK, Peter H, Farkas J, Gschwentner R, Sorokin
    L, Romani N, Stoitzner P. 2008. The lymph vessel network in mouse skin visualised
    with antibodies against the hyaluronan receptor LYVE-1. Immunobiology. 213(9–10),
    715–728.
  mla: Tripp, Christoph, et al. “The Lymph Vessel Network in Mouse Skin Visualised
    with Antibodies against the Hyaluronan Receptor LYVE-1.” <i>Immunobiology</i>,
    vol. 213, no. 9–10, Elsevier, 2008, pp. 715–28, doi:<a href="https://doi.org/10.1016/j.imbio.2008.07.025">10.1016/j.imbio.2008.07.025</a>.
  short: C. Tripp, B. Haid, V. Flacher, M.K. Sixt, H. Peter, J. Farkas, R. Gschwentner,
    L. Sorokin, N. Romani, P. Stoitzner, Immunobiology 213 (2008) 715–728.
date_created: 2018-12-11T12:06:02Z
date_published: 2008-08-30T00:00:00Z
date_updated: 2026-05-29T09:17:45Z
day: '30'
doi: 10.1016/j.imbio.2008.07.025
extern: '1'
external_id:
  pmid:
  - '18926287'
intvolume: '       213'
issue: 9-10
language:
- iso: eng
month: '08'
oa_version: None
page: 715 - 728
pmid: 1
publication: Immunobiology
publication_identifier:
  eissn:
  - 1878-3279
  issn:
  - 0171-2985
publication_status: published
publisher: Elsevier
publist_id: '2182'
status: public
title: The lymph vessel network in mouse skin visualised with antibodies against the
  hyaluronan receptor LYVE-1
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 213
year: '2008'
...