---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '18942'
abstract:
- lang: eng
  text: Photosynthesis is among the first processes negatively affected by environmental
    cues and its performance directly determines plant cell fitness and ultimately
    crop yield. Primarily sites of photosynthesis, chloroplasts are unique sites also
    for the biosynthesis of precursors of the growth regulator auxin and for sensing
    environmental stress, but their role in intracellular auxin homeostasis, vital
    for plant growth and survival in changing environments, remains poorly understood.
    Here, we identified two ATP-binding cassette (ABC) subfamily B transporters, ABCB28
    and ABCB29, which export auxin across the chloroplast envelope to the cytosol
    in a concerted action in vivo. Moreover, we provide evidence for an auxin biosynthesis
    pathway in Arabidopsis thaliana chloroplasts. The overexpression of ABCB28 and
    ABCB29 influenced stomatal regulation and resulted in significantly improved water
    use efficiency and survival rates during salt and drought stresses. Our results
    suggest that chloroplast auxin production and transport contribute to stomata
    regulation for conserving water upon salt stress. ABCB28 and ABCB29 integrate
    photosynthesis and auxin signals and as such hold great potential to improve the
    adaptation potential of crops to environmental cues.
article_number: '7'
article_processing_charge: Yes
article_type: original
author:
- first_name: Prashanth
  full_name: Tamizhselvan, Prashanth
  last_name: Tamizhselvan
- first_name: Sharmila
  full_name: Madhavan, Sharmila
  last_name: Madhavan
- first_name: Christian
  full_name: Constan-Aguilar, Christian
  last_name: Constan-Aguilar
- first_name: Eman Ryad
  full_name: Elrefaay, Eman Ryad
  last_name: Elrefaay
- first_name: Jie
  full_name: Liu, Jie
  last_name: Liu
- first_name: Aleš
  full_name: Pěnčík, Aleš
  last_name: Pěnčík
- first_name: Ondřej
  full_name: Novák, Ondřej
  last_name: Novák
- first_name: Albert
  full_name: Cairó, Albert
  last_name: Cairó
- first_name: Mónika
  full_name: Hrtyan, Mónika
  id: 45A71A74-F248-11E8-B48F-1D18A9856A87
  last_name: Hrtyan
- first_name: Markus
  full_name: Geisler, Markus
  last_name: Geisler
- first_name: Vanesa Beatriz
  full_name: Tognetti, Vanesa Beatriz
  last_name: Tognetti
citation:
  ama: Tamizhselvan P, Madhavan S, Constan-Aguilar C, et al. Chloroplast auxin efflux
    mediated by ABCB28 and ABCB29 fine-tunes salt and drought stress responses in
    Arabidopsis. <i>Plants</i>. 2023;13(1). doi:<a href="https://doi.org/10.3390/plants13010007">10.3390/plants13010007</a>
  apa: Tamizhselvan, P., Madhavan, S., Constan-Aguilar, C., Elrefaay, E. R., Liu,
    J., Pěnčík, A., … Tognetti, V. B. (2023). Chloroplast auxin efflux mediated by
    ABCB28 and ABCB29 fine-tunes salt and drought stress responses in Arabidopsis.
    <i>Plants</i>. MDPI. <a href="https://doi.org/10.3390/plants13010007">https://doi.org/10.3390/plants13010007</a>
  chicago: Tamizhselvan, Prashanth, Sharmila Madhavan, Christian Constan-Aguilar,
    Eman Ryad Elrefaay, Jie Liu, Aleš Pěnčík, Ondřej Novák, et al. “Chloroplast Auxin
    Efflux Mediated by ABCB28 and ABCB29 Fine-Tunes Salt and Drought Stress Responses
    in Arabidopsis.” <i>Plants</i>. MDPI, 2023. <a href="https://doi.org/10.3390/plants13010007">https://doi.org/10.3390/plants13010007</a>.
  ieee: P. Tamizhselvan <i>et al.</i>, “Chloroplast auxin efflux mediated by ABCB28
    and ABCB29 fine-tunes salt and drought stress responses in Arabidopsis,” <i>Plants</i>,
    vol. 13, no. 1. MDPI, 2023.
  ista: Tamizhselvan P, Madhavan S, Constan-Aguilar C, Elrefaay ER, Liu J, Pěnčík
    A, Novák O, Cairó A, Hrtyan M, Geisler M, Tognetti VB. 2023. Chloroplast auxin
    efflux mediated by ABCB28 and ABCB29 fine-tunes salt and drought stress responses
    in Arabidopsis. Plants. 13(1), 7.
  mla: Tamizhselvan, Prashanth, et al. “Chloroplast Auxin Efflux Mediated by ABCB28
    and ABCB29 Fine-Tunes Salt and Drought Stress Responses in Arabidopsis.” <i>Plants</i>,
    vol. 13, no. 1, 7, MDPI, 2023, doi:<a href="https://doi.org/10.3390/plants13010007">10.3390/plants13010007</a>.
  short: P. Tamizhselvan, S. Madhavan, C. Constan-Aguilar, E.R. Elrefaay, J. Liu,
    A. Pěnčík, O. Novák, A. Cairó, M. Hrtyan, M. Geisler, V.B. Tognetti, Plants 13
    (2023).
date_created: 2025-01-29T09:03:56Z
date_published: 2023-12-19T00:00:00Z
date_updated: 2025-01-29T09:07:53Z
day: '19'
ddc:
- '580'
department:
- _id: EvBe
doi: 10.3390/plants13010007
external_id:
  pmid:
  - '38202315'
file:
- access_level: open_access
  checksum: 97efcefa8151d69343b0b641630c86ee
  content_type: application/pdf
  creator: dernst
  date_created: 2025-01-29T09:06:51Z
  date_updated: 2025-01-29T09:06:51Z
  file_id: '18943'
  file_name: 2023_Plants_Tamizhselvan.pdf
  file_size: 6231778
  relation: main_file
  success: 1
file_date_updated: 2025-01-29T09:06:51Z
fulldoi: https://doi.org/10.3390/plants13010007
has_accepted_license: '1'
intvolume: '        13'
issue: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plants
publication_identifier:
  eissn:
  - 2223-7747
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Chloroplast auxin efflux mediated by ABCB28 and ABCB29 fine-tunes salt and
  drought stress responses in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 13
year: '2023'
...
---
_id: '7582'
abstract:
- lang: eng
  text: Small RNAs (smRNA, 19–25 nucleotides long), which are transcribed by RNA polymerase
    II, regulate the expression of genes involved in a multitude of processes in eukaryotes.
    miRNA biogenesis and the proteins involved in the biogenesis pathway differ across
    plant and animal lineages. The major proteins constituting the biogenesis pathway,
    namely, the Dicers (DCL/DCR) and Argonautes (AGOs), have been extensively studied.
    However, the accessory proteins (DAWDLE (DDL), SERRATE (SE), and TOUGH (TGH))
    of the pathway that differs across the two lineages remain largely uncharacterized.
    We present the first detailed report on the molecular evolution and divergence
    of these proteins across eukaryotes. Although DDL is present in eukaryotes and
    prokaryotes, SE and TGH appear to be specific to eukaryotes. The addition/deletion
    of specific domains and/or domain-specific sequence divergence in the three proteins
    points to the observed functional divergence of these proteins across the two
    lineages, which correlates with the differences in miRNA length across the two
    lineages. Our data enhance the current understanding of the structure–function
    relationship of these proteins and reveals previous unexplored crucial residues
    in the three proteins that can be used as a basis for further functional characterization.
    The data presented here on the number of miRNAs in crown eukaryotic lineages are
    consistent with the notion of the expansion of the number of miRNA-coding genes
    in animal and plant lineages correlating with organismal complexity. Whether this
    difference in functionally correlates with the diversification (or presence/absence)
    of the three proteins studied here or the miRNA signaling in the plant and animal
    lineages is unclear. Based on our results of the three proteins studied here and
    previously available data concerning the evolution of miRNA genes in the plant
    and animal lineages, we believe that miRNAs probably evolved once in the ancestor
    to crown eukaryotes and have diversified independently in the eukaryotes.
article_number: '299'
article_processing_charge: No
article_type: original
author:
- first_name: Taraka Ramji
  full_name: Moturu, Taraka Ramji
  last_name: Moturu
- first_name: Sansrity
  full_name: Sinha, Sansrity
  last_name: Sinha
- first_name: Hymavathi
  full_name: Salava, Hymavathi
  last_name: Salava
- first_name: Sravankumar
  full_name: Thula, Sravankumar
  last_name: Thula
- first_name: Tomasz
  full_name: Nodzyński, Tomasz
  last_name: Nodzyński
- first_name: Radka Svobodová
  full_name: Vařeková, Radka Svobodová
  last_name: Vařeková
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Sibu
  full_name: Simon, Sibu
  id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
  last_name: Simon
  orcid: 0000-0002-1998-6741
citation:
  ama: Moturu TR, Sinha S, Salava H, et al. Molecular evolution and diversification
    of proteins involved in miRNA maturation pathway. <i>Plants</i>. 2020;9(3). doi:<a
    href="https://doi.org/10.3390/plants9030299">10.3390/plants9030299</a>
  apa: Moturu, T. R., Sinha, S., Salava, H., Thula, S., Nodzyński, T., Vařeková, R.
    S., … Simon, S. (2020). Molecular evolution and diversification of proteins involved
    in miRNA maturation pathway. <i>Plants</i>. MDPI. <a href="https://doi.org/10.3390/plants9030299">https://doi.org/10.3390/plants9030299</a>
  chicago: Moturu, Taraka Ramji, Sansrity Sinha, Hymavathi Salava, Sravankumar Thula,
    Tomasz Nodzyński, Radka Svobodová Vařeková, Jiří Friml, and Sibu Simon. “Molecular
    Evolution and Diversification of Proteins Involved in MiRNA Maturation Pathway.”
    <i>Plants</i>. MDPI, 2020. <a href="https://doi.org/10.3390/plants9030299">https://doi.org/10.3390/plants9030299</a>.
  ieee: T. R. Moturu <i>et al.</i>, “Molecular evolution and diversification of proteins
    involved in miRNA maturation pathway,” <i>Plants</i>, vol. 9, no. 3. MDPI, 2020.
  ista: Moturu TR, Sinha S, Salava H, Thula S, Nodzyński T, Vařeková RS, Friml J,
    Simon S. 2020. Molecular evolution and diversification of proteins involved in
    miRNA maturation pathway. Plants. 9(3), 299.
  mla: Moturu, Taraka Ramji, et al. “Molecular Evolution and Diversification of Proteins
    Involved in MiRNA Maturation Pathway.” <i>Plants</i>, vol. 9, no. 3, 299, MDPI,
    2020, doi:<a href="https://doi.org/10.3390/plants9030299">10.3390/plants9030299</a>.
  short: T.R. Moturu, S. Sinha, H. Salava, S. Thula, T. Nodzyński, R.S. Vařeková,
    J. Friml, S. Simon, Plants 9 (2020).
corr_author: '1'
date_created: 2020-03-15T23:00:52Z
date_published: 2020-03-01T00:00:00Z
date_updated: 2026-04-02T14:35:47Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.3390/plants9030299
ec_funded: 1
external_id:
  isi:
  - '000525315000035'
  pmid:
  - '32121542'
file:
- access_level: open_access
  checksum: 6d5af3e17266a48996b4af4e67e88a85
  content_type: application/pdf
  creator: dernst
  date_created: 2020-03-23T13:37:00Z
  date_updated: 2020-07-14T12:48:00Z
  file_id: '7614'
  file_name: 2020_Plants_Moturu.pdf
  file_size: 2373484
  relation: main_file
file_date_updated: 2020-07-14T12:48:00Z
fulldoi: https://doi.org/10.3390/plants9030299
has_accepted_license: '1'
intvolume: '         9'
isi: 1
issue: '3'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: Plants
publication_identifier:
  eissn:
  - 2223-7747
publication_status: published
publisher: MDPI
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular evolution and diversification of proteins involved in miRNA maturation
  pathway
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 9
year: '2020'
...