@article{19963,
  abstract     = {The acquisition of cellular identity requires large-scale alterations in cellular state. The noncanonical proteasome activator PSME3 is known to regulate diverse cellular processes, but its importance for differentiation remains unclear. Here, we demonstrate that PSME3 binds dynamically to highly active promoters over the course of differentiation. However, loss of PSME3 does not globally affect mRNA transcription. We find instead that PSME3 influences the levels of several adhesion-related proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility and myoblast differentiation in a proteasome-independent manner. Our findings reveal several new facets of PSME3 functionality and highlight its importance for the differentiation of myogenic cells.},
  author       = {Kuhn, Kenneth D and Cho, Ukrae H. and Hetzer, Martin W},
  issn         = {2575-1077},
  journal      = {Life Science Alliance},
  number       = {9},
  publisher    = {Embo Press},
  title        = {{PSME3 regulates migration and differentiation of myoblasts}},
  doi          = {10.26508/lsa.202503208},
  volume       = {8},
  year         = {2025},
}

@article{10144,
  abstract     = {FGFs and their high-affinity receptors (FGFRs) play key roles in development, tissue repair, and disease. Because FGFRs bind overlapping sets of ligands, their individual functions cannot be determined using ligand stimulation. Here, we generated a light-activated FGFR2 variant (OptoR2) to selectively activate signaling by the major FGFR in keratinocytes. Illumination of OptoR2-expressing HEK 293T cells activated FGFR signaling with remarkable temporal precision and promoted cell migration and proliferation. In murine and human keratinocytes, OptoR2 activation rapidly induced the classical FGFR signaling pathways and expression of FGF target genes. Surprisingly, multi-level counter-regulation occurred in keratinocytes in vitro and in transgenic mice in vivo, including OptoR2 down-regulation and loss of responsiveness to light activation. These results demonstrate unexpected cell type-specific limitations of optogenetic FGFRs in long-term in vitro and in vivo settings and highlight the complex consequences of transferring optogenetic cell signaling tools into their relevant cellular contexts.},
  author       = {Rauschendorfer, Theresa and Gurri, Selina and Heggli, Irina and Maddaluno, Luigi and Meyer, Michael and Inglés Prieto, Álvaro and Janovjak, Harald L and Werner, Sabine},
  issn         = {2575-1077},
  journal      = {Life Science Alliance},
  number       = {11},
  publisher    = {Life Science Alliance},
  title        = {{Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice}},
  doi          = {10.26508/lsa.202101100},
  volume       = {4},
  year         = {2021},
}