[{"date_updated":"2026-05-20T08:38:04Z","day":"01","pmid":1,"fulldoi":"https://doi.org/10.26508/lsa.202503208","scopus_import":"1","ddc":["570"],"type":"journal_article","license":"https://creativecommons.org/licenses/by/4.0/","status":"public","APC_amount":"4215,38 EUR","has_accepted_license":"1","project":[{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"}],"issue":"9","year":"2025","file":[{"file_name":"2025_LifeScienceAlliance_Kuhn.pdf","relation":"main_file","content_type":"application/pdf","creator":"dernst","date_updated":"2025-12-30T09:17:09Z","file_size":5471288,"checksum":"591d47aa39fc969986c7d3b966890f5f","access_level":"open_access","file_id":"20904","date_created":"2025-12-30T09:17:09Z","success":1}],"PlanS_conform":"1","department":[{"_id":"MaHe"}],"acknowledgement":"All proteomics analysis was done by the ISTA LSF Mass Spectrometry Service: Ewelina Dutkiewicz-Kopczynska processed the samples (digest and cleanup); Bella Bruszel optimized the acquisition methods, acquired the data, and performed all searches; and Armel Nicolas provided pre- and post-project consulting and post-processed the search results using a development version of their data analysis package, proteoCraft (publication pending). The authors would like to thank Saki for their clarity of thought and insight, as well as Dr. Lorenzo Puri and the members of his laboratory for invaluable discussions relating to the project. This research was further supported by the Lab Support Facility and the Imaging and Optics Facility of ISTA.","quality_controlled":"1","OA_type":"gold","_id":"19963","publisher":"Embo Press","article_type":"original","title":"PSME3 regulates migration and differentiation of myoblasts","acknowledged_ssus":[{"_id":"LifeSc"},{"_id":"Bio"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"09","volume":8,"oa":1,"date_published":"2025-09-01T00:00:00Z","OA_place":"publisher","publication":"Life Science Alliance","intvolume":"         8","citation":{"ista":"Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation of myoblasts. Life Science Alliance. 8(9), e202503208.","chicago":"Kuhn, Kenneth D, Ukrae H. Cho, and Martin Hetzer. “PSME3 Regulates Migration and Differentiation of Myoblasts.” <i>Life Science Alliance</i>. Embo Press, 2025. <a href=\"https://doi.org/10.26508/lsa.202503208\">https://doi.org/10.26508/lsa.202503208</a>.","ieee":"K. D. Kuhn, U. H. Cho, and M. Hetzer, “PSME3 regulates migration and differentiation of myoblasts,” <i>Life Science Alliance</i>, vol. 8, no. 9. Embo Press, 2025.","short":"K.D. Kuhn, U.H. Cho, M. Hetzer, Life Science Alliance 8 (2025).","ama":"Kuhn KD, Cho UH, Hetzer M. PSME3 regulates migration and differentiation of myoblasts. <i>Life Science Alliance</i>. 2025;8(9). doi:<a href=\"https://doi.org/10.26508/lsa.202503208\">10.26508/lsa.202503208</a>","apa":"Kuhn, K. D., Cho, U. H., &#38; Hetzer, M. (2025). PSME3 regulates migration and differentiation of myoblasts. <i>Life Science Alliance</i>. Embo Press. <a href=\"https://doi.org/10.26508/lsa.202503208\">https://doi.org/10.26508/lsa.202503208</a>","mla":"Kuhn, Kenneth D., et al. “PSME3 Regulates Migration and Differentiation of Myoblasts.” <i>Life Science Alliance</i>, vol. 8, no. 9, e202503208, Embo Press, 2025, doi:<a href=\"https://doi.org/10.26508/lsa.202503208\">10.26508/lsa.202503208</a>."},"isi":1,"DOAJ_listed":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","corr_author":"1","language":[{"iso":"eng"}],"external_id":{"isi":["001511452100001"],"pmid":["40537284"]},"author":[{"last_name":"Kuhn","first_name":"Kenneth D","full_name":"Kuhn, Kenneth D","id":"7deed7e0-0133-11f0-8590-c4600b08d0f4"},{"last_name":"Cho","first_name":"Ukrae H.","full_name":"Cho, Ukrae H."},{"last_name":"Hetzer","id":"86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed","orcid":"0000-0002-2111-992X","full_name":"Hetzer, Martin W","first_name":"Martin W"}],"abstract":[{"lang":"eng","text":"The acquisition of cellular identity requires large-scale alterations in cellular state. The noncanonical proteasome activator PSME3 is known to regulate diverse cellular processes, but its importance for differentiation remains unclear. Here, we demonstrate that PSME3 binds dynamically to highly active promoters over the course of differentiation. However, loss of PSME3 does not globally affect mRNA transcription. We find instead that PSME3 influences the levels of several adhesion-related proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility and myoblast differentiation in a proteasome-independent manner. Our findings reveal several new facets of PSME3 functionality and highlight its importance for the differentiation of myogenic cells."}],"file_date_updated":"2025-12-30T09:17:09Z","article_number":"e202503208","oa_version":"Published Version","publication_identifier":{"eissn":["2575-1077"]},"date_created":"2025-07-06T22:01:22Z","publication_status":"published","doi":"10.26508/lsa.202503208","article_processing_charge":"Yes"},{"acknowledgement":"We thank Connor Richterich and Patricia Reinert, ETH Zurich, for invaluable experimental help; Manuela Pérez Berlanga, University Zurich, for help with the confocal imaging; Lukas Fischer for help with electrical engineering; Thomas Hennek, Sol Taguinod, and Dr. Stephan Sonntag, EPIC Phenomics Center, ETH Zürich, for the generation and maintenance of K14-OptoR2 mice; and Dr. Petra Boukamp, Leibniz Institute, Düsseldorf, Germany, for early-passage HaCaT keratinocytes. This work was supported by the ETH Zurich (grant ETH-06 15-1 to S Werner and L Maddaluno), the Swiss National Science Foundation (grant 31003B-189364 to S Werner), and a Marie Curie postdoctoral fellowship from the European Union (to L Maddaluno).","quality_controlled":"1","year":"2021","file":[{"success":1,"date_created":"2021-10-18T14:48:06Z","access_level":"open_access","file_id":"10152","checksum":"89fb95b211dbe8678809e7cca4626952","file_size":2055981,"date_updated":"2021-10-18T14:48:06Z","creator":"cchlebak","content_type":"application/pdf","relation":"main_file","file_name":"2021_LifeScAlliance_Rauschendorfer.pdf"}],"type":"journal_article","status":"public","has_accepted_license":"1","issue":"11","date_updated":"2024-10-09T21:01:02Z","day":"21","pmid":1,"fulldoi":"https://doi.org/10.26508/lsa.202101100","scopus_import":"1","ddc":["576"],"external_id":{"pmid":["34548382"]},"abstract":[{"lang":"eng","text":"FGFs and their high-affinity receptors (FGFRs) play key roles in development, tissue repair, and disease. Because FGFRs bind overlapping sets of ligands, their individual functions cannot be determined using ligand stimulation. Here, we generated a light-activated FGFR2 variant (OptoR2) to selectively activate signaling by the major FGFR in keratinocytes. Illumination of OptoR2-expressing HEK 293T cells activated FGFR signaling with remarkable temporal precision and promoted cell migration and proliferation. In murine and human keratinocytes, OptoR2 activation rapidly induced the classical FGFR signaling pathways and expression of FGF target genes. Surprisingly, multi-level counter-regulation occurred in keratinocytes in vitro and in transgenic mice in vivo, including OptoR2 down-regulation and loss of responsiveness to light activation. These results demonstrate unexpected cell type-specific limitations of optogenetic FGFRs in long-term in vitro and in vivo settings and highlight the complex consequences of transferring optogenetic cell signaling tools into their relevant cellular contexts."}],"author":[{"full_name":"Rauschendorfer, Theresa","first_name":"Theresa","last_name":"Rauschendorfer"},{"full_name":"Gurri, Selina","first_name":"Selina","last_name":"Gurri"},{"full_name":"Heggli, Irina","first_name":"Irina","last_name":"Heggli"},{"last_name":"Maddaluno","full_name":"Maddaluno, Luigi","first_name":"Luigi"},{"first_name":"Michael","full_name":"Meyer, Michael","last_name":"Meyer"},{"full_name":"Inglés Prieto, Álvaro","first_name":"Álvaro","id":"2A9DB292-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5409-8571","last_name":"Inglés Prieto"},{"last_name":"Janovjak","first_name":"Harald L","full_name":"Janovjak, Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315"},{"last_name":"Werner","full_name":"Werner, Sabine","first_name":"Sabine"}],"file_date_updated":"2021-10-18T14:48:06Z","article_number":"e202101100","oa_version":"Published Version","publication_identifier":{"eissn":["2575-1077"]},"date_created":"2021-10-17T22:01:16Z","publication_status":"published","doi":"10.26508/lsa.202101100","article_processing_charge":"Yes","publication":"Life Science Alliance","citation":{"chicago":"Rauschendorfer, Theresa, Selina Gurri, Irina Heggli, Luigi Maddaluno, Michael Meyer, Álvaro Inglés Prieto, Harald L Janovjak, and Sabine Werner. “Acute and Chronic Effects of a Light-Activated FGF Receptor in Keratinocytes in Vitro and in Mice.” <i>Life Science Alliance</i>. Life Science Alliance, 2021. <a href=\"https://doi.org/10.26508/lsa.202101100\">https://doi.org/10.26508/lsa.202101100</a>.","ieee":"T. Rauschendorfer <i>et al.</i>, “Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice,” <i>Life Science Alliance</i>, vol. 4, no. 11. Life Science Alliance, 2021.","ista":"Rauschendorfer T, Gurri S, Heggli I, Maddaluno L, Meyer M, Inglés Prieto Á, Janovjak HL, Werner S. 2021. Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice. Life Science Alliance. 4(11), e202101100.","ama":"Rauschendorfer T, Gurri S, Heggli I, et al. Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice. <i>Life Science Alliance</i>. 2021;4(11). doi:<a href=\"https://doi.org/10.26508/lsa.202101100\">10.26508/lsa.202101100</a>","short":"T. Rauschendorfer, S. Gurri, I. Heggli, L. Maddaluno, M. Meyer, Á. Inglés Prieto, H.L. Janovjak, S. Werner, Life Science Alliance 4 (2021).","apa":"Rauschendorfer, T., Gurri, S., Heggli, I., Maddaluno, L., Meyer, M., Inglés Prieto, Á., … Werner, S. (2021). Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice. <i>Life Science Alliance</i>. Life Science Alliance. <a href=\"https://doi.org/10.26508/lsa.202101100\">https://doi.org/10.26508/lsa.202101100</a>","mla":"Rauschendorfer, Theresa, et al. “Acute and Chronic Effects of a Light-Activated FGF Receptor in Keratinocytes in Vitro and in Mice.” <i>Life Science Alliance</i>, vol. 4, no. 11, e202101100, Life Science Alliance, 2021, doi:<a href=\"https://doi.org/10.26508/lsa.202101100\">10.26508/lsa.202101100</a>."},"intvolume":"         4","extern":"1","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","corr_author":"1","language":[{"iso":"eng"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","short":"CC BY (4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"month":"09","volume":4,"oa":1,"date_published":"2021-09-21T00:00:00Z","_id":"10144","publisher":"Life Science Alliance","article_type":"original","title":"Acute and chronic effects of a light-activated FGF receptor in keratinocytes in vitro and in mice"}]