---
APC_amount: 4215,38 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '19963'
abstract:
- lang: eng
text: The acquisition of cellular identity requires large-scale alterations in cellular
state. The noncanonical proteasome activator PSME3 is known to regulate diverse
cellular processes, but its importance for differentiation remains unclear. Here,
we demonstrate that PSME3 binds dynamically to highly active promoters over the
course of differentiation. However, loss of PSME3 does not globally affect mRNA
transcription. We find instead that PSME3 influences the levels of several adhesion-related
proteins and acts upstream of the HSP90 co-chaperone NUDC to regulate cell motility
and myoblast differentiation in a proteasome-independent manner. Our findings
reveal several new facets of PSME3 functionality and highlight its importance
for the differentiation of myogenic cells.
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
acknowledgement: 'All proteomics analysis was done by the ISTA LSF Mass Spectrometry
Service: Ewelina Dutkiewicz-Kopczynska processed the samples (digest and cleanup);
Bella Bruszel optimized the acquisition methods, acquired the data, and performed
all searches; and Armel Nicolas provided pre- and post-project consulting and post-processed
the search results using a development version of their data analysis package, proteoCraft
(publication pending). The authors would like to thank Saki for their clarity of
thought and insight, as well as Dr. Lorenzo Puri and the members of his laboratory
for invaluable discussions relating to the project. This research was further supported
by the Lab Support Facility and the Imaging and Optics Facility of ISTA.'
article_number: e202503208
article_processing_charge: Yes
article_type: original
author:
- first_name: Kenneth D
full_name: Kuhn, Kenneth D
id: 7deed7e0-0133-11f0-8590-c4600b08d0f4
last_name: Kuhn
- first_name: Ukrae H.
full_name: Cho, Ukrae H.
last_name: Cho
- first_name: Martin W
full_name: Hetzer, Martin W
id: 86c0d31b-b4eb-11ec-ac5a-eae7b2e135ed
last_name: Hetzer
orcid: 0000-0002-2111-992X
citation:
ama: Kuhn KD, Cho UH, Hetzer M. PSME3 regulates migration and differentiation of
myoblasts. Life Science Alliance. 2025;8(9). doi:10.26508/lsa.202503208
apa: Kuhn, K. D., Cho, U. H., & Hetzer, M. (2025). PSME3 regulates migration
and differentiation of myoblasts. Life Science Alliance. Embo Press. https://doi.org/10.26508/lsa.202503208
chicago: Kuhn, Kenneth D, Ukrae H. Cho, and Martin Hetzer. “PSME3 Regulates Migration
and Differentiation of Myoblasts.” Life Science Alliance. Embo Press, 2025.
https://doi.org/10.26508/lsa.202503208.
ieee: K. D. Kuhn, U. H. Cho, and M. Hetzer, “PSME3 regulates migration and differentiation
of myoblasts,” Life Science Alliance, vol. 8, no. 9. Embo Press, 2025.
ista: Kuhn KD, Cho UH, Hetzer M. 2025. PSME3 regulates migration and differentiation
of myoblasts. Life Science Alliance. 8(9), e202503208.
mla: Kuhn, Kenneth D., et al. “PSME3 Regulates Migration and Differentiation of
Myoblasts.” Life Science Alliance, vol. 8, no. 9, e202503208, Embo Press,
2025, doi:10.26508/lsa.202503208.
short: K.D. Kuhn, U.H. Cho, M. Hetzer, Life Science Alliance 8 (2025).
corr_author: '1'
date_created: 2025-07-06T22:01:22Z
date_published: 2025-09-01T00:00:00Z
date_updated: 2026-05-20T08:38:04Z
day: '01'
ddc:
- '570'
department:
- _id: MaHe
doi: 10.26508/lsa.202503208
external_id:
isi:
- '001511452100001'
pmid:
- '40537284'
file:
- access_level: open_access
checksum: 591d47aa39fc969986c7d3b966890f5f
content_type: application/pdf
creator: dernst
date_created: 2025-12-30T09:17:09Z
date_updated: 2025-12-30T09:17:09Z
file_id: '20904'
file_name: 2025_LifeScienceAlliance_Kuhn.pdf
file_size: 5471288
relation: main_file
success: 1
file_date_updated: 2025-12-30T09:17:09Z
fulldoi: https://doi.org/10.26508/lsa.202503208
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '9'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: B67AFEDC-15C9-11EA-A837-991A96BB2854
name: IST Austria Open Access Fund
publication: Life Science Alliance
publication_identifier:
eissn:
- 2575-1077
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: PSME3 regulates migration and differentiation of myoblasts
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2025'
...
---
_id: '10144'
abstract:
- lang: eng
text: FGFs and their high-affinity receptors (FGFRs) play key roles in development,
tissue repair, and disease. Because FGFRs bind overlapping sets of ligands, their
individual functions cannot be determined using ligand stimulation. Here, we generated
a light-activated FGFR2 variant (OptoR2) to selectively activate signaling by
the major FGFR in keratinocytes. Illumination of OptoR2-expressing HEK 293T cells
activated FGFR signaling with remarkable temporal precision and promoted cell
migration and proliferation. In murine and human keratinocytes, OptoR2 activation
rapidly induced the classical FGFR signaling pathways and expression of FGF target
genes. Surprisingly, multi-level counter-regulation occurred in keratinocytes
in vitro and in transgenic mice in vivo, including OptoR2 down-regulation and
loss of responsiveness to light activation. These results demonstrate unexpected
cell type-specific limitations of optogenetic FGFRs in long-term in vitro and
in vivo settings and highlight the complex consequences of transferring optogenetic
cell signaling tools into their relevant cellular contexts.
acknowledgement: We thank Connor Richterich and Patricia Reinert, ETH Zurich, for
invaluable experimental help; Manuela Pérez Berlanga, University Zurich, for help
with the confocal imaging; Lukas Fischer for help with electrical engineering; Thomas
Hennek, Sol Taguinod, and Dr. Stephan Sonntag, EPIC Phenomics Center, ETH Zürich,
for the generation and maintenance of K14-OptoR2 mice; and Dr. Petra Boukamp, Leibniz
Institute, Düsseldorf, Germany, for early-passage HaCaT keratinocytes. This work
was supported by the ETH Zurich (grant ETH-06 15-1 to S Werner and L Maddaluno),
the Swiss National Science Foundation (grant 31003B-189364 to S Werner), and a Marie
Curie postdoctoral fellowship from the European Union (to L Maddaluno).
article_number: e202101100
article_processing_charge: Yes
article_type: original
author:
- first_name: Theresa
full_name: Rauschendorfer, Theresa
last_name: Rauschendorfer
- first_name: Selina
full_name: Gurri, Selina
last_name: Gurri
- first_name: Irina
full_name: Heggli, Irina
last_name: Heggli
- first_name: Luigi
full_name: Maddaluno, Luigi
last_name: Maddaluno
- first_name: Michael
full_name: Meyer, Michael
last_name: Meyer
- first_name: Álvaro
full_name: Inglés Prieto, Álvaro
id: 2A9DB292-F248-11E8-B48F-1D18A9856A87
last_name: Inglés Prieto
orcid: 0000-0002-5409-8571
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
- first_name: Sabine
full_name: Werner, Sabine
last_name: Werner
citation:
ama: Rauschendorfer T, Gurri S, Heggli I, et al. Acute and chronic effects of a
light-activated FGF receptor in keratinocytes in vitro and in mice. Life Science
Alliance. 2021;4(11). doi:10.26508/lsa.202101100
apa: Rauschendorfer, T., Gurri, S., Heggli, I., Maddaluno, L., Meyer, M., Inglés
Prieto, Á., … Werner, S. (2021). Acute and chronic effects of a light-activated
FGF receptor in keratinocytes in vitro and in mice. Life Science Alliance.
Life Science Alliance. https://doi.org/10.26508/lsa.202101100
chicago: Rauschendorfer, Theresa, Selina Gurri, Irina Heggli, Luigi Maddaluno, Michael
Meyer, Álvaro Inglés Prieto, Harald L Janovjak, and Sabine Werner. “Acute and
Chronic Effects of a Light-Activated FGF Receptor in Keratinocytes in Vitro and
in Mice.” Life Science Alliance. Life Science Alliance, 2021. https://doi.org/10.26508/lsa.202101100.
ieee: T. Rauschendorfer et al., “Acute and chronic effects of a light-activated
FGF receptor in keratinocytes in vitro and in mice,” Life Science Alliance,
vol. 4, no. 11. Life Science Alliance, 2021.
ista: Rauschendorfer T, Gurri S, Heggli I, Maddaluno L, Meyer M, Inglés Prieto Á,
Janovjak HL, Werner S. 2021. Acute and chronic effects of a light-activated FGF
receptor in keratinocytes in vitro and in mice. Life Science Alliance. 4(11),
e202101100.
mla: Rauschendorfer, Theresa, et al. “Acute and Chronic Effects of a Light-Activated
FGF Receptor in Keratinocytes in Vitro and in Mice.” Life Science Alliance,
vol. 4, no. 11, e202101100, Life Science Alliance, 2021, doi:10.26508/lsa.202101100.
short: T. Rauschendorfer, S. Gurri, I. Heggli, L. Maddaluno, M. Meyer, Á. Inglés
Prieto, H.L. Janovjak, S. Werner, Life Science Alliance 4 (2021).
corr_author: '1'
date_created: 2021-10-17T22:01:16Z
date_published: 2021-09-21T00:00:00Z
date_updated: 2024-10-09T21:01:02Z
day: '21'
ddc:
- '576'
doi: 10.26508/lsa.202101100
extern: '1'
external_id:
pmid:
- '34548382'
file:
- access_level: open_access
checksum: 89fb95b211dbe8678809e7cca4626952
content_type: application/pdf
creator: cchlebak
date_created: 2021-10-18T14:48:06Z
date_updated: 2021-10-18T14:48:06Z
file_id: '10152'
file_name: 2021_LifeScAlliance_Rauschendorfer.pdf
file_size: 2055981
relation: main_file
success: 1
file_date_updated: 2021-10-18T14:48:06Z
fulldoi: https://doi.org/10.26508/lsa.202101100
has_accepted_license: '1'
intvolume: ' 4'
issue: '11'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
publication: Life Science Alliance
publication_identifier:
eissn:
- 2575-1077
publication_status: published
publisher: Life Science Alliance
quality_controlled: '1'
scopus_import: '1'
status: public
title: Acute and chronic effects of a light-activated FGF receptor in keratinocytes
in vitro and in mice
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2021'
...