[{"ddc":["570"],"oa_version":"Published Version","main_file_link":[{"url":"https://doi.org/10.1016/j.isci.2026.115192","open_access":"1"}],"type":"journal_article","doi":"10.1016/j.isci.2026.115192","publication_status":"epub_ahead","month":"03","title":"Distribution and functional significance of rodent cerebellar glycogen","pmid":1,"publication_identifier":{"eissn":["2589-0042"]},"date_updated":"2026-06-18T08:32:22Z","date_published":"2026-03-17T00:00:00Z","article_type":"original","oa":1,"abstract":[{"lang":"eng","text":"The mammalian brain stores glucose, the main circulating energy substrate, as glycogen. In rodents, the cerebellum contains relatively high glycogen levels, yet its cellular and subcellular distribution remains poorly defined. Using monoclonal antibodies against glycogen, we examined its distribution in the mouse cerebellar cortex. Glycogen was predominantly localized to Bergmann glia (BG) processes in the molecular layer and was also detected in Purkinje cells (PCs), the principal cerebellar neurons. To assess the functional significance of cerebellar glycogen, we analyzed behavior in mice lacking glycogen synthase 1 (Gys1) in BG or PCs using a floxed Gys1 line. Gys1 deficiency in either PCs or GFAP-positive cells reduced anxiety-like behavior, whereas combined deletion caused PC degeneration and ataxia. These findings reveal a critical role for glycogen metabolism in both astrocytes and neurons in cerebellar function."}],"date_created":"2026-03-29T22:07:07Z","_id":"21502","author":[{"last_name":"Akther","full_name":"Akther, Sonam","first_name":"Sonam"},{"last_name":"Lee","first_name":"Ashley Bomin","full_name":"Lee, Ashley Bomin"},{"last_name":"Konno","full_name":"Konno, Ayumu","first_name":"Ayumu"},{"first_name":"Antonis","full_name":"Asiminas, Antonis","last_name":"Asiminas"},{"last_name":"Vittani","first_name":"Marta","full_name":"Vittani, Marta"},{"first_name":"Tsuneko","full_name":"Mishima, Tsuneko","last_name":"Mishima"},{"last_name":"Hirai","first_name":"Hirokazu","full_name":"Hirai, Hirokazu"},{"full_name":"Meehan, Claire Francesca","first_name":"Claire Francesca","last_name":"Meehan"},{"full_name":"Duran, Jordi","first_name":"Jordi","last_name":"Duran"},{"first_name":"Joan","full_name":"Guinovart, Joan","last_name":"Guinovart"},{"first_name":"Hitoshi","full_name":"Ashida, Hitoshi","last_name":"Ashida"},{"first_name":"Tsuyoshi","full_name":"Morita, Tsuyoshi","last_name":"Morita"},{"last_name":"Baba","full_name":"Baba, Otto","first_name":"Otto"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto"},{"full_name":"Nedergaard, Maiken","first_name":"Maiken","last_name":"Nedergaard"},{"full_name":"Hirase, Hajime","first_name":"Hajime","last_name":"Hirase"}],"volume":29,"day":"17","intvolume":"        29","OA_place":"publisher","article_processing_charge":"Yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"This work was supported by the Novo Nordisk Foundation (NNFOC0058058, H. Hirase), the Danmarks Frie Forskningsfond (0134-00107B and 5283-00069A, H.Hirase), the Lundbeck Foundation, Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research (KAKENHI) program (22K06454/24H01221, A.K.; 23K27482, H.Hirai), the Japan Agency for Medical Research and Development (AMED) Brain Mapping by Integrated Neurotechnologies for Disease Studies (Brain/MINDS) (JP21dm0207111, H. Hirai), AMED Brain/MINDS 2.0 (JP23wm0625001 and JP24wm0625103, H. Hirai), and grants from the Spanish Ministerio de Ciencia e Innovación (MCIU/FEDER/AEI) (PID2020-118699 GB-100, J.D.) and the Fundación Ramón Areces (J.D.). Sonam Akther has been supported by the RIKEN IPA fellowship. We are thankful to Dr. Yuki Oe for his support in the initial stage of this study and to Dan Xue for his help with the graphical abstract. We thank Dr. Pia Weikop for providing CTN research infrastructure. The authors declare no competing financial interests.","language":[{"iso":"eng"}],"DOAJ_listed":"1","year":"2026","publisher":"Elsevier","issue":"4","article_number":"115192","quality_controlled":"1","citation":{"ieee":"S. Akther <i>et al.</i>, “Distribution and functional significance of rodent cerebellar glycogen,” <i>iScience</i>, vol. 29, no. 4. Elsevier, 2026.","mla":"Akther, Sonam, et al. “Distribution and Functional Significance of Rodent Cerebellar Glycogen.” <i>IScience</i>, vol. 29, no. 4, 115192, Elsevier, 2026, doi:<a href=\"https://doi.org/10.1016/j.isci.2026.115192\">10.1016/j.isci.2026.115192</a>.","ama":"Akther S, Lee AB, Konno A, et al. Distribution and functional significance of rodent cerebellar glycogen. <i>iScience</i>. 2026;29(4). doi:<a href=\"https://doi.org/10.1016/j.isci.2026.115192\">10.1016/j.isci.2026.115192</a>","chicago":"Akther, Sonam, Ashley Bomin Lee, Ayumu Konno, Antonis Asiminas, Marta Vittani, Tsuneko Mishima, Hirokazu Hirai, et al. “Distribution and Functional Significance of Rodent Cerebellar Glycogen.” <i>IScience</i>. Elsevier, 2026. <a href=\"https://doi.org/10.1016/j.isci.2026.115192\">https://doi.org/10.1016/j.isci.2026.115192</a>.","ista":"Akther S, Lee AB, Konno A, Asiminas A, Vittani M, Mishima T, Hirai H, Meehan CF, Duran J, Guinovart J, Ashida H, Morita T, Baba O, Shigemoto R, Nedergaard M, Hirase H. 2026. Distribution and functional significance of rodent cerebellar glycogen. iScience. 29(4), 115192.","short":"S. Akther, A.B. Lee, A. Konno, A. Asiminas, M. Vittani, T. Mishima, H. Hirai, C.F. Meehan, J. Duran, J. Guinovart, H. Ashida, T. Morita, O. Baba, R. Shigemoto, M. Nedergaard, H. Hirase, IScience 29 (2026).","apa":"Akther, S., Lee, A. B., Konno, A., Asiminas, A., Vittani, M., Mishima, T., … Hirase, H. (2026). Distribution and functional significance of rodent cerebellar glycogen. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2026.115192\">https://doi.org/10.1016/j.isci.2026.115192</a>"},"status":"public","publication":"iScience","OA_type":"gold","external_id":{"pmid":["41890976"]},"scopus_import":"1","department":[{"_id":"RySh"}]},{"isi":1,"type":"journal_article","oa_version":"Published Version","file_date_updated":"2023-10-09T07:23:46Z","publication_status":"published","has_accepted_license":"1","doi":"10.1016/j.isci.2023.107840","project":[{"call_identifier":"H2020","grant_number":"754411","name":"ISTplus - Postdoctoral Fellowships","_id":"260C2330-B435-11E9-9278-68D0E5697425"},{"grant_number":"M03318","name":"Functional Advantages of Critical Brain Dynamics","_id":"eb943429-77a9-11ec-83b8-9f471cdf5c67"}],"ddc":["570"],"oa":1,"abstract":[{"text":"Sleep plays a key role in preserving brain function, keeping the brain network in a state that ensures optimal computational capabilities. Empirical evidence indicates that such a state is consistent with criticality, where scale-free neuronal avalanches emerge. However, the relationship between sleep, emergent avalanches, and criticality remains poorly understood. Here we fully characterize the critical behavior of avalanches during sleep, and study their relationship with the sleep macro- and micro-architecture, in particular the cyclic alternating pattern (CAP). We show that avalanche size and duration distributions exhibit robust power laws with exponents approximately equal to −3/2 e −2, respectively. Importantly, we find that sizes scale as a power law of the durations, and that all critical exponents for neuronal avalanches obey robust scaling relations, which are consistent with the mean-field directed percolation universality class. Our analysis demonstrates that avalanche dynamics depends on the position within the NREM-REM cycles, with the avalanche density increasing in the descending phases and decreasing in the ascending phases of sleep cycles. Moreover, we show that, within NREM sleep, avalanche occurrence correlates with CAP activation phases, particularly A1, which are the expression of slow wave sleep propensity and have been proposed to be beneficial for cognitive processes. The results suggest that neuronal avalanches, and thus tuning to criticality, actively contribute to sleep development and play a role in preserving network function. Such findings, alongside characterization of the universality class for avalanches, open new avenues to the investigation of functional role of criticality during sleep with potential clinical application.</jats:p><jats:sec><jats:title>Significance statement</jats:title><jats:p>We fully characterize the critical behavior of neuronal avalanches during sleep, and show that avalanches follow precise scaling laws that are consistent with the mean-field directed percolation universality class. The analysis provides first evidence of a functional relationship between avalanche occurrence, slow-wave sleep dynamics, sleep stage transitions and occurrence of CAP phase A during NREM sleep. Because CAP is considered one of the major guardians of NREM sleep that allows the brain to dynamically react to external perturbation and contributes to the cognitive consolidation processes occurring in sleep, our observations suggest that neuronal avalanches at criticality are associated with flexible response to external inputs and to cognitive processes, a key assumption of the critical brain hypothesis.","lang":"eng"}],"month":"10","file":[{"date_created":"2023-10-09T07:23:46Z","creator":"dernst","date_updated":"2023-10-09T07:23:46Z","file_name":"2023_iScience_Scarpetta.pdf","file_id":"14412","content_type":"application/pdf","success":1,"checksum":"f499836af172ecc9865de4bb41fa99d1","relation":"main_file","file_size":4872708,"access_level":"open_access"}],"date_updated":"2025-04-14T07:44:00Z","publication_identifier":{"eissn":["2589-0042"]},"date_published":"2023-10-20T00:00:00Z","article_type":"original","title":"Criticality of neuronal avalanches in human sleep and their relationship with sleep macro- and micro-architecture","pmid":1,"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"day":"20","_id":"12487","author":[{"first_name":"Silvia","full_name":"Scarpetta, Silvia","last_name":"Scarpetta"},{"full_name":"Morrisi, Niccolò","first_name":"Niccolò","last_name":"Morrisi"},{"full_name":"Mutti, Carlotta","first_name":"Carlotta","last_name":"Mutti"},{"first_name":"Nicoletta","full_name":"Azzi, Nicoletta","last_name":"Azzi"},{"first_name":"Irene","full_name":"Trippi, Irene","last_name":"Trippi"},{"last_name":"Ciliento","first_name":"Rosario","full_name":"Ciliento, Rosario"},{"first_name":"Ilenia","full_name":"Apicella, Ilenia","last_name":"Apicella"},{"last_name":"Messuti","full_name":"Messuti, Giovanni","first_name":"Giovanni"},{"first_name":"Marianna","full_name":"Angiolelli, Marianna","last_name":"Angiolelli"},{"id":"A057D288-3E88-11E9-986D-0CF4E5697425","last_name":"Lombardi","orcid":"0000-0003-2623-5249","full_name":"Lombardi, Fabrizio","first_name":"Fabrizio"},{"full_name":"Parrino, Liborio","first_name":"Liborio","last_name":"Parrino"},{"last_name":"Vaudano","first_name":"Anna Elisabetta","full_name":"Vaudano, Anna Elisabetta"}],"page":"107840","date_created":"2023-02-02T10:50:17Z","volume":26,"external_id":{"isi":["001082331200001"],"pmid":["37766992"]},"citation":{"chicago":"Scarpetta, Silvia, Niccolò Morrisi, Carlotta Mutti, Nicoletta Azzi, Irene Trippi, Rosario Ciliento, Ilenia Apicella, et al. “Criticality of Neuronal Avalanches in Human Sleep and Their Relationship with Sleep Macro- and Micro-Architecture.” <i>IScience</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.isci.2023.107840\">https://doi.org/10.1016/j.isci.2023.107840</a>.","ista":"Scarpetta S, Morrisi N, Mutti C, Azzi N, Trippi I, Ciliento R, Apicella I, Messuti G, Angiolelli M, Lombardi F, Parrino L, Vaudano AE. 2023. Criticality of neuronal avalanches in human sleep and their relationship with sleep macro- and micro-architecture. iScience. 26(10), 107840.","short":"S. Scarpetta, N. Morrisi, C. Mutti, N. Azzi, I. Trippi, R. Ciliento, I. Apicella, G. Messuti, M. Angiolelli, F. Lombardi, L. Parrino, A.E. Vaudano, IScience 26 (2023) 107840.","apa":"Scarpetta, S., Morrisi, N., Mutti, C., Azzi, N., Trippi, I., Ciliento, R., … Vaudano, A. E. (2023). Criticality of neuronal avalanches in human sleep and their relationship with sleep macro- and micro-architecture. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2023.107840\">https://doi.org/10.1016/j.isci.2023.107840</a>","ieee":"S. Scarpetta <i>et al.</i>, “Criticality of neuronal avalanches in human sleep and their relationship with sleep macro- and micro-architecture,” <i>iScience</i>, vol. 26, no. 10. Elsevier, p. 107840, 2023.","mla":"Scarpetta, Silvia, et al. “Criticality of Neuronal Avalanches in Human Sleep and Their Relationship with Sleep Macro- and Micro-Architecture.” <i>IScience</i>, vol. 26, no. 10, Elsevier, 2023, p. 107840, doi:<a href=\"https://doi.org/10.1016/j.isci.2023.107840\">10.1016/j.isci.2023.107840</a>.","ama":"Scarpetta S, Morrisi N, Mutti C, et al. Criticality of neuronal avalanches in human sleep and their relationship with sleep macro- and micro-architecture. <i>iScience</i>. 2023;26(10):107840. doi:<a href=\"https://doi.org/10.1016/j.isci.2023.107840\">10.1016/j.isci.2023.107840</a>"},"quality_controlled":"1","publication":"iScience","status":"public","scopus_import":"1","department":[{"_id":"GaTk"}],"ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"FL acknowledges support from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie Grant Agreement No. 754411, and from the Austrian Science Fund (FWF) under the Lise Meitner fellowship No. PT1013M03318. IA acknowledges financial support from the MIUR PRIN 2017WZFTZP.","language":[{"iso":"eng"}],"intvolume":"        26","article_processing_charge":"Yes","issue":"10","publisher":"Elsevier","year":"2023"},{"abstract":[{"lang":"eng","text":"Mitochondrial networks remodel their connectivity, content, and subcellular localization to support optimized energy production in conditions of increased environmental or cellular stress. Microglia rely on mitochondria to respond to these stressors, however our knowledge about mitochondrial networks and their adaptations in microglia in vivo is limited. Here, we generate a mouse model that selectively labels mitochondria in microglia. We identify that mitochondrial networks are more fragmented with increased content and perinuclear localization in vitro vs. in vivo. Mitochondrial networks adapt similarly in microglia closest to the injury site after optic nerve crush. Preventing microglial UCP2 increase after injury by selective knockout induces cellular stress. This results in mitochondrial hyperfusion in male microglia, a phenotype absent in females due to circulating estrogens. Our results establish the foundation for mitochondrial network analysis of microglia in vivo, emphasizing the importance of mitochondrial-based sex effects of microglia in other pathologies."}],"oa":1,"file":[{"success":1,"file_size":8197935,"access_level":"open_access","relation":"main_file","checksum":"be1a560efdd96d20712311f4fc54aac2","file_id":"14497","date_updated":"2023-11-07T08:53:21Z","content_type":"application/pdf","file_name":"2023_iScience_Maes.pdf","date_created":"2023-11-07T08:53:21Z","creator":"dernst"}],"month":"10","date_published":"2023-10-20T00:00:00Z","article_type":"original","publication_identifier":{"eissn":["2589-0042"]},"date_updated":"2024-10-09T21:07:01Z","pmid":1,"title":"Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout","type":"journal_article","isi":1,"oa_version":"Published Version","publication_status":"published","file_date_updated":"2023-11-07T08:53:21Z","has_accepted_license":"1","doi":"10.1016/j.isci.2023.107780","ddc":["570"],"external_id":{"pmid":["37731609"],"isi":["001080403500001"]},"status":"public","publication":"iScience","citation":{"chicago":"Maes, Margaret E, Gloria Colombo, Florianne E Schoot Uiterkamp, Felix Sternberg, Alessandro Venturino, Elena E. Pohl, and Sandra Siegert. “Mitochondrial Network Adaptations of Microglia Reveal Sex-Specific Stress Response after Injury and UCP2 Knockout.” <i>IScience</i>. Elsevier, 2023. <a href=\"https://doi.org/10.1016/j.isci.2023.107780\">https://doi.org/10.1016/j.isci.2023.107780</a>.","ista":"Maes ME, Colombo G, Schoot Uiterkamp FE, Sternberg F, Venturino A, Pohl EE, Siegert S. 2023. Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. iScience. 26(10), 107780.","apa":"Maes, M. E., Colombo, G., Schoot Uiterkamp, F. E., Sternberg, F., Venturino, A., Pohl, E. E., &#38; Siegert, S. (2023). Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2023.107780\">https://doi.org/10.1016/j.isci.2023.107780</a>","short":"M.E. Maes, G. Colombo, F.E. Schoot Uiterkamp, F. Sternberg, A. Venturino, E.E. Pohl, S. Siegert, IScience 26 (2023).","mla":"Maes, Margaret E., et al. “Mitochondrial Network Adaptations of Microglia Reveal Sex-Specific Stress Response after Injury and UCP2 Knockout.” <i>IScience</i>, vol. 26, no. 10, 107780, Elsevier, 2023, doi:<a href=\"https://doi.org/10.1016/j.isci.2023.107780\">10.1016/j.isci.2023.107780</a>.","ieee":"M. E. Maes <i>et al.</i>, “Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout,” <i>iScience</i>, vol. 26, no. 10. Elsevier, 2023.","ama":"Maes ME, Colombo G, Schoot Uiterkamp FE, et al. Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockout. <i>iScience</i>. 2023;26(10). doi:<a href=\"https://doi.org/10.1016/j.isci.2023.107780\">10.1016/j.isci.2023.107780</a>"},"quality_controlled":"1","department":[{"_id":"SaSi"}],"scopus_import":"1","acknowledgement":"We thank the Scientific Service Units (SSU) of ISTA through resources provided by the Imaging and Optics Facility (IOF), the Lab Support Facility (LSF), and the Pre-Clinical Facility (PCF) team, specifically Sonja Haslinger and Michael Schunn for excellent mouse colony management and support. This research was supported by the FWF Sonderforschungsbereich F83 (to E.E.P). We thank Bálint Nagy, Ryan John A. Cubero, Marco Benevento and all members of the Siegert group for constant feedback on the project and article.","language":[{"iso":"eng"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","intvolume":"        26","article_processing_charge":"Yes","article_number":"107780","issue":"10","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"PreCl"}],"year":"2023","publisher":"Elsevier","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"day":"20","corr_author":"1","author":[{"id":"3838F452-F248-11E8-B48F-1D18A9856A87","last_name":"Maes","orcid":"0000-0001-9642-1085","full_name":"Maes, Margaret E","first_name":"Margaret E"},{"last_name":"Colombo","id":"3483CF6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9434-8902","first_name":"Gloria","full_name":"Colombo, Gloria"},{"id":"3526230C-F248-11E8-B48F-1D18A9856A87","last_name":"Schoot Uiterkamp","first_name":"Florianne E","full_name":"Schoot Uiterkamp, Florianne E"},{"full_name":"Sternberg, Felix","first_name":"Felix","last_name":"Sternberg"},{"last_name":"Venturino","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2356-9403","first_name":"Alessandro","full_name":"Venturino, Alessandro"},{"first_name":"Elena E.","full_name":"Pohl, Elena E.","last_name":"Pohl"},{"id":"36ACD32E-F248-11E8-B48F-1D18A9856A87","last_name":"Siegert","orcid":"0000-0001-8635-0877","first_name":"Sandra","full_name":"Siegert, Sandra"}],"_id":"14363","date_created":"2023-09-24T22:01:11Z","volume":26},{"type":"journal_article","isi":1,"oa_version":"Published Version","file_date_updated":"2022-07-04T08:19:25Z","publication_status":"published","doi":"10.1016/j.isci.2022.104580","has_accepted_license":"1","project":[{"name":"Microglia action towards neuronal circuit formation and function in health and disease","_id":"25D4A630-B435-11E9-9278-68D0E5697425","grant_number":"715571","call_identifier":"H2020"},{"name":"IST Austria Open Access Fund","_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854"},{"name":"How human microglia shape developing neurons during health and inflammation","_id":"9B99D380-BA93-11EA-9121-9846C619BF3A","grant_number":"SC19-017"}],"ddc":["610"],"oa":1,"abstract":[{"text":"Cerebral organoids differentiated from human-induced pluripotent stem cells (hiPSC) provide a unique opportunity to investigate brain development. However, organoids usually lack microglia, brain-resident immune cells, which are present in the early embryonic brain and participate in neuronal circuit development. Here, we find IBA1+ microglia-like cells alongside retinal cups between week 3 and 4 in 2.5D culture with an unguided retinal organoid differentiation protocol. Microglia do not infiltrate the neuroectoderm and instead enrich within non-pigmented, 3D-cystic compartments that develop in parallel to the 3D-retinal organoids. When we guide the retinal organoid differentiation with low-dosed BMP4, we prevent cup development and enhance microglia and 3D-cysts formation. Mass spectrometry identifies these 3D-cysts to express mesenchymal and epithelial markers. We confirmed this microglia-preferred environment also within the unguided protocol, providing insight into microglial behavior and migration and offer a model to study how they enter and distribute within the human brain.","lang":"eng"}],"month":"07","file":[{"creator":"cchlebak","date_created":"2022-07-04T08:19:25Z","content_type":"application/pdf","date_updated":"2022-07-04T08:19:25Z","file_id":"11480","file_name":"2022_iScience_Bartalska.pdf","relation":"main_file","file_size":19400048,"access_level":"open_access","checksum":"a470b74e1b3796c710189c81a4cd4329","success":1}],"publication_identifier":{"eissn":["2589-0042"]},"date_updated":"2026-04-07T11:51:43Z","date_published":"2022-07-15T00:00:00Z","article_type":"original","title":"A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation","pmid":1,"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"corr_author":"1","day":"15","_id":"11478","author":[{"full_name":"Bartalska, Katarina","first_name":"Katarina","last_name":"Bartalska","id":"4D883232-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Hübschmann","id":"32B7C918-F248-11E8-B48F-1D18A9856A87","full_name":"Hübschmann, Verena","first_name":"Verena"},{"full_name":"Korkut, Medina","first_name":"Medina","orcid":"0000-0003-4309-2251","last_name":"Korkut","id":"4B51CE74-F248-11E8-B48F-1D18A9856A87"},{"last_name":"Cubero","id":"850B2E12-9CD4-11E9-837F-E719E6697425","first_name":"Ryan J","full_name":"Cubero, Ryan J","orcid":"0000-0003-0002-1867"},{"orcid":"0000-0003-2356-9403","full_name":"Venturino, Alessandro","first_name":"Alessandro","last_name":"Venturino","id":"41CB84B2-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Rössler, Karl","first_name":"Karl","last_name":"Rössler"},{"full_name":"Czech, Thomas","first_name":"Thomas","last_name":"Czech"},{"full_name":"Siegert, Sandra","first_name":"Sandra","orcid":"0000-0001-8635-0877","last_name":"Siegert","id":"36ACD32E-F248-11E8-B48F-1D18A9856A87"}],"date_created":"2022-07-03T22:01:33Z","related_material":{"record":[{"id":"12117","status":"public","relation":"other"},{"id":"20074","relation":"dissertation_contains","status":"public"}]},"volume":25,"external_id":{"pmid":["35789843"],"isi":["000830428500005"]},"quality_controlled":"1","citation":{"mla":"Bartalska, Katarina, et al. “A Systematic Characterization of Microglia-like Cell Occurrence during Retinal Organoid Differentiation.” <i>IScience</i>, vol. 25, no. 7, 104580, Elsevier, 2022, doi:<a href=\"https://doi.org/10.1016/j.isci.2022.104580\">10.1016/j.isci.2022.104580</a>.","ieee":"K. Bartalska <i>et al.</i>, “A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation,” <i>iScience</i>, vol. 25, no. 7. Elsevier, 2022.","ama":"Bartalska K, Hübschmann V, Korkut M, et al. A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. <i>iScience</i>. 2022;25(7). doi:<a href=\"https://doi.org/10.1016/j.isci.2022.104580\">10.1016/j.isci.2022.104580</a>","chicago":"Bartalska, Katarina, Verena Hübschmann, Medina Korkut, Ryan J Cubero, Alessandro Venturino, Karl Rössler, Thomas Czech, and Sandra Siegert. “A Systematic Characterization of Microglia-like Cell Occurrence during Retinal Organoid Differentiation.” <i>IScience</i>. Elsevier, 2022. <a href=\"https://doi.org/10.1016/j.isci.2022.104580\">https://doi.org/10.1016/j.isci.2022.104580</a>.","ista":"Bartalska K, Hübschmann V, Korkut M, Cubero RJ, Venturino A, Rössler K, Czech T, Siegert S. 2022. A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. iScience. 25(7), 104580.","apa":"Bartalska, K., Hübschmann, V., Korkut, M., Cubero, R. J., Venturino, A., Rössler, K., … Siegert, S. (2022). A systematic characterization of microglia-like cell occurrence during retinal organoid differentiation. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2022.104580\">https://doi.org/10.1016/j.isci.2022.104580</a>","short":"K. Bartalska, V. Hübschmann, M. Korkut, R.J. Cubero, A. Venturino, K. Rössler, T. Czech, S. Siegert, IScience 25 (2022)."},"publication":"iScience","status":"public","scopus_import":"1","department":[{"_id":"SaSi"}],"ec_funded":1,"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","acknowledgement":"We thank the scientific service units at ISTA, specifically the lab support facility and imaging & optics facility for their support; Nicolas Armel for performing the Mass Spectrometry. We thank Alexandra Lang and Tanja Peilnsteiner for their help in human brain tissue collection, Rouven Schulz for his insights into the functional assays We thank all members of the Siegert group for constant feedback on the project and Margaret Maes, Rouven Schulz, and Marco Benevento for feedback on the manuscript. This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation program (grant No. 715571 to S.S.) and from the Gesellschaft für Forschungsförderung Niederösterreich (grant No. Sc19-017 to V.H.).","language":[{"iso":"eng"}],"article_processing_charge":"Yes","intvolume":"        25","issue":"7","article_number":"104580","year":"2022","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publisher":"Elsevier"},{"_id":"11974","author":[{"first_name":"Susanne","full_name":"Reischauer, Susanne","last_name":"Reischauer"},{"id":"93e5e5b2-0da6-11ed-8a41-af589a024726","last_name":"Pieber","first_name":"Bartholomäus","full_name":"Pieber, Bartholomäus","orcid":"0000-0001-8689-388X"}],"date_created":"2022-08-25T10:31:44Z","volume":24,"day":"19","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","language":[{"iso":"eng"}],"article_processing_charge":"No","intvolume":"        24","article_number":"102209","issue":"3","year":"2021","publisher":"Elsevier","quality_controlled":"1","citation":{"ama":"Reischauer S, Pieber B. Emerging concepts in photocatalytic organic synthesis. <i>iScience</i>. 2021;24(3). doi:<a href=\"https://doi.org/10.1016/j.isci.2021.102209\">10.1016/j.isci.2021.102209</a>","mla":"Reischauer, Susanne, and Bartholomäus Pieber. “Emerging Concepts in Photocatalytic Organic Synthesis.” <i>IScience</i>, vol. 24, no. 3, 102209, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.isci.2021.102209\">10.1016/j.isci.2021.102209</a>.","ieee":"S. Reischauer and B. Pieber, “Emerging concepts in photocatalytic organic synthesis,” <i>iScience</i>, vol. 24, no. 3. Elsevier, 2021.","short":"S. Reischauer, B. Pieber, IScience 24 (2021).","apa":"Reischauer, S., &#38; Pieber, B. (2021). Emerging concepts in photocatalytic organic synthesis. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2021.102209\">https://doi.org/10.1016/j.isci.2021.102209</a>","chicago":"Reischauer, Susanne, and Bartholomäus Pieber. “Emerging Concepts in Photocatalytic Organic Synthesis.” <i>IScience</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.isci.2021.102209\">https://doi.org/10.1016/j.isci.2021.102209</a>.","ista":"Reischauer S, Pieber B. 2021. Emerging concepts in photocatalytic organic synthesis. iScience. 24(3), 102209."},"publication":"iScience","status":"public","scopus_import":"1","extern":"1","main_file_link":[{"url":"https://doi.org/10.1016/j.isci.2021.102209","open_access":"1"}],"type":"journal_article","oa_version":"Published Version","publication_status":"published","doi":"10.1016/j.isci.2021.102209","month":"03","publication_identifier":{"eissn":["2589-0042"]},"date_updated":"2024-10-14T12:05:29Z","article_type":"review","date_published":"2021-03-19T00:00:00Z","title":"Emerging concepts in photocatalytic organic synthesis","oa":1,"abstract":[{"lang":"eng","text":"Visible light photocatalysis has become a powerful tool in organic synthesis that uses photons as traceless, sustainable reagents. Most of the activities in the field focus on the development of new reactions via common photoredox cycles, but recently a number of exciting new concepts and strategies entered less charted territories. We survey approaches that enable the use of longer wavelengths and show that the wavelength and intensity of photons are import parameters that enable tuning of the reactivity of a photocatalyst to control or change the selectivity of chemical reactions. In addition, we discuss recent efforts to substitute strong reductants, such as elemental lithium and sodium, by light and technological advances in the field."}]},{"abstract":[{"lang":"eng","text":"Cryo-EM grid preparation is an important bottleneck in protein structure determination, especially for membrane proteins, typically requiring screening of a large number of conditions. We systematically investigated the effects of buffer components, blotting conditions and grid types on the outcome of grid preparation of five different membrane protein samples. Aggregation was the most common type of problem which was addressed by changing detergents, salt concentration or reconstitution of proteins into nanodiscs or amphipols. We show that the optimal concentration of detergent is between 0.05 and 0.4% and that the presence of a low concentration of detergent with a high critical micellar concentration protects the proteins from denaturation at the air-water interface. Furthermore, we discuss the strategies for achieving an adequate ice thickness, particle coverage and orientation distribution on free ice and on support films. Our findings provide a clear roadmap for comprehensive screening of conditions for cryo-EM grid preparation of membrane proteins."}],"oa":1,"file":[{"success":1,"checksum":"50585447386fe5842f07ab9b3a66e7e9","file_size":7431411,"relation":"main_file","access_level":"open_access","date_created":"2021-03-03T07:38:14Z","creator":"dernst","file_id":"9219","file_name":"2021_iScience_Kampjut.pdf","content_type":"application/pdf","date_updated":"2021-03-03T07:38:14Z"}],"month":"03","pmid":1,"title":"Cryo-EM grid optimization for membrane proteins","article_type":"original","date_published":"2021-03-19T00:00:00Z","publication_identifier":{"eissn":["2589-0042"]},"date_updated":"2026-04-02T14:00:19Z","oa_version":"Published Version","type":"journal_article","isi":1,"has_accepted_license":"1","publication_status":"published","doi":"10.1016/j.isci.2021.102139","file_date_updated":"2021-03-03T07:38:14Z","ddc":["570"],"project":[{"call_identifier":"H2020","grant_number":"665385","_id":"2564DBCA-B435-11E9-9278-68D0E5697425","name":"International IST Doctoral Program"}],"publication":"iScience","status":"public","quality_controlled":"1","citation":{"ama":"Kampjut D, Steiner J, Sazanov LA. Cryo-EM grid optimization for membrane proteins. <i>iScience</i>. 2021;24(3). doi:<a href=\"https://doi.org/10.1016/j.isci.2021.102139\">10.1016/j.isci.2021.102139</a>","ieee":"D. Kampjut, J. Steiner, and L. A. Sazanov, “Cryo-EM grid optimization for membrane proteins,” <i>iScience</i>, vol. 24, no. 3. Elsevier, 2021.","mla":"Kampjut, Domen, et al. “Cryo-EM Grid Optimization for Membrane Proteins.” <i>IScience</i>, vol. 24, no. 3, 102139, Elsevier, 2021, doi:<a href=\"https://doi.org/10.1016/j.isci.2021.102139\">10.1016/j.isci.2021.102139</a>.","apa":"Kampjut, D., Steiner, J., &#38; Sazanov, L. A. (2021). Cryo-EM grid optimization for membrane proteins. <i>IScience</i>. Elsevier. <a href=\"https://doi.org/10.1016/j.isci.2021.102139\">https://doi.org/10.1016/j.isci.2021.102139</a>","short":"D. Kampjut, J. Steiner, L.A. Sazanov, IScience 24 (2021).","ista":"Kampjut D, Steiner J, Sazanov LA. 2021. Cryo-EM grid optimization for membrane proteins. iScience. 24(3), 102139.","chicago":"Kampjut, Domen, Julia Steiner, and Leonid A Sazanov. “Cryo-EM Grid Optimization for Membrane Proteins.” <i>IScience</i>. Elsevier, 2021. <a href=\"https://doi.org/10.1016/j.isci.2021.102139\">https://doi.org/10.1016/j.isci.2021.102139</a>."},"external_id":{"pmid":["33665558"],"isi":["000631646000012"]},"ec_funded":1,"department":[{"_id":"LeSa"}],"scopus_import":"1","article_processing_charge":"No","intvolume":"        24","acknowledgement":"We thank the Electron Microscopy Facilities at the Institute of Science and Technology Austria and at the Vienna Biocenter for providing access and training for the electron microscopes. This project has received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie Grant Agreement no. 665385 .","language":[{"iso":"eng"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","acknowledged_ssus":[{"_id":"EM-Fac"}],"publisher":"Elsevier","year":"2021","article_number":"102139","issue":"3","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"day":"19","date_created":"2021-02-28T23:01:24Z","author":[{"last_name":"Kampjut","id":"37233050-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6018-3422","full_name":"Kampjut, Domen","first_name":"Domen"},{"last_name":"Steiner","id":"3BB67EB0-F248-11E8-B48F-1D18A9856A87","full_name":"Steiner, Julia","first_name":"Julia","orcid":"0000-0003-0493-3775"},{"orcid":"0000-0002-0977-7989","first_name":"Leonid A","full_name":"Sazanov, Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","last_name":"Sazanov"}],"_id":"9205","volume":24}]