---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '17436'
abstract:
- lang: eng
  text: The auxin signaling molecule controls a variety of growth and developmental
    processes in land plants. Auxin regulates gene expression through a nuclear auxin
    signaling pathway (NAP) consisting of the ubiquitin ligase auxin receptor TIR1/AFB,
    its Aux/IAA degradation substrate, and DNA-binding ARF transcription factors.
    Although extensive qualitative understanding of the pathway and its interactions
    has been obtained, mostly by studying the flowering plant Arabidopsis thaliana,
    it remains unknown how these translate to quantitative system behavior in vivo,
    a problem that is confounded by the large NAP gene families in most species. Here,
    we used the minimal NAP of the liverwort Marchantia polymorpha to quantitatively
    map NAP protein accumulation and dynamics in vivo through the use of knockin fluorescent
    fusion proteins. Beyond revealing the dynamic native accumulation profile of the
    entire NAP protein network, we discovered that the two central ARFs, MpARF1 and
    MpARF2, are proteasomally degraded. This auxin-independent degradation tunes ARF
    protein stoichiometry to favor gene activation, thereby reprogramming auxin response
    during the developmental progression. Thus, quantitative analysis of the entire
    NAP has enabled us to identify ARF degradation and the stoichiometries of activator
    and repressor ARFs as a potential mechanism for controlling gemma germination.
acknowledgement: "We are grateful to Iris Nieuwland and Neri van Laar for experimental
  support. No conflict of interest declared.\r\nThis work was supported by the Netherlands
  Organisation for Scientific Research, the Netherlands (grants ALWOP.402 and OCENW.M20.031
  to J.W.B.) and the Human Frontiers Research Program (grant RGP0015/2022 to D.W.)."
article_number: '101039'
article_processing_charge: Yes
article_type: original
author:
- first_name: Shubhajit
  full_name: Das, Shubhajit
  id: b08969a4-f2a5-11ed-b6c4-ff0f10b7d0be
  last_name: Das
- first_name: Martijn
  full_name: De Roij, Martijn
  last_name: De Roij
- first_name: Simon
  full_name: Bellows, Simon
  last_name: Bellows
- first_name: Melissa Dipp
  full_name: Alvarez, Melissa Dipp
  last_name: Alvarez
- first_name: Sumanth
  full_name: Mutte, Sumanth
  last_name: Mutte
- first_name: Wouter
  full_name: Kohlen, Wouter
  last_name: Kohlen
- first_name: Etienne
  full_name: Farcot, Etienne
  last_name: Farcot
- first_name: Dolf
  full_name: Weijers, Dolf
  last_name: Weijers
- first_name: Jan Willem
  full_name: Borst, Jan Willem
  last_name: Borst
citation:
  ama: Das S, De Roij M, Bellows S, et al. Quantitative imaging reveals the role of
    MpARF proteasomal degradation during gemma germination. <i>Plant Communications</i>.
    2024;5(11). doi:<a href="https://doi.org/10.1016/j.xplc.2024.101039">10.1016/j.xplc.2024.101039</a>
  apa: Das, S., De Roij, M., Bellows, S., Alvarez, M. D., Mutte, S., Kohlen, W., …
    Borst, J. W. (2024). Quantitative imaging reveals the role of MpARF proteasomal
    degradation during gemma germination. <i>Plant Communications</i>. Elsevier. <a
    href="https://doi.org/10.1016/j.xplc.2024.101039">https://doi.org/10.1016/j.xplc.2024.101039</a>
  chicago: Das, Shubhajit, Martijn De Roij, Simon Bellows, Melissa Dipp Alvarez, Sumanth
    Mutte, Wouter Kohlen, Etienne Farcot, Dolf Weijers, and Jan Willem Borst. “Quantitative
    Imaging Reveals the Role of MpARF Proteasomal Degradation during Gemma Germination.”
    <i>Plant Communications</i>. Elsevier, 2024. <a href="https://doi.org/10.1016/j.xplc.2024.101039">https://doi.org/10.1016/j.xplc.2024.101039</a>.
  ieee: S. Das <i>et al.</i>, “Quantitative imaging reveals the role of MpARF proteasomal
    degradation during gemma germination,” <i>Plant Communications</i>, vol. 5, no.
    11. Elsevier, 2024.
  ista: Das S, De Roij M, Bellows S, Alvarez MD, Mutte S, Kohlen W, Farcot E, Weijers
    D, Borst JW. 2024. Quantitative imaging reveals the role of MpARF proteasomal
    degradation during gemma germination. Plant Communications. 5(11), 101039.
  mla: Das, Shubhajit, et al. “Quantitative Imaging Reveals the Role of MpARF Proteasomal
    Degradation during Gemma Germination.” <i>Plant Communications</i>, vol. 5, no.
    11, 101039, Elsevier, 2024, doi:<a href="https://doi.org/10.1016/j.xplc.2024.101039">10.1016/j.xplc.2024.101039</a>.
  short: S. Das, M. De Roij, S. Bellows, M.D. Alvarez, S. Mutte, W. Kohlen, E. Farcot,
    D. Weijers, J.W. Borst, Plant Communications 5 (2024).
date_created: 2024-08-18T22:01:04Z
date_published: 2024-11-11T00:00:00Z
date_updated: 2025-01-09T12:26:55Z
day: '11'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2024.101039
external_id:
  pmid:
  - '38988072'
file:
- access_level: open_access
  checksum: 38cabc1042ac7fb70e6c4c510eba88fc
  content_type: application/pdf
  creator: dernst
  date_created: 2025-01-09T12:25:32Z
  date_updated: 2025-01-09T12:25:32Z
  file_id: '18811'
  file_name: 2024_PlantCommunications_Das.pdf
  file_size: 4970540
  relation: main_file
  success: 1
file_date_updated: 2025-01-09T12:25:32Z
has_accepted_license: '1'
intvolume: '         5'
issue: '11'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Communications
publication_identifier:
  eissn:
  - 2590-3462
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Quantitative imaging reveals the role of MpARF proteasomal degradation during
  gemma germination
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2024'
...
---
_id: '13209'
abstract:
- lang: eng
  text: The phytohormone auxin plays central roles in many growth and developmental
    processes in plants. Development of chemical tools targeting the auxin pathway
    is useful for both plant biology and agriculture. Here we reveal that naproxen,
    a synthetic compound with anti-inflammatory activity in humans, acts as an auxin
    transport inhibitor targeting PIN-FORMED (PIN) transporters in plants. Physiological
    experiments indicate that exogenous naproxen treatment affects pleiotropic auxin-regulated
    developmental processes. Additional cellular and biochemical evidence indicates
    that naproxen suppresses auxin transport, specifically PIN-mediated auxin efflux.
    Moreover, biochemical and structural analyses confirm that naproxen binds directly
    to PIN1 protein via the same binding cavity as the indole-3-acetic acid substrate.
    Thus, by combining cellular, biochemical, and structural approaches, this study
    clearly establishes that naproxen is a PIN inhibitor and elucidates the underlying
    mechanisms. Further use of this compound may advance our understanding of the
    molecular mechanisms of PIN-mediated auxin transport and expand our toolkit in
    auxin biology and agriculture.
acknowledgement: "This work was supported by the Strategic Priority Research Program
  of the Chinese Academy of Sciences (XDB37020103 to Linfeng Sun); research funds
  from the Center for Advanced Interdisciplinary Science\r\nand Biomedicine of IHM,
  Division of Life Sciences and Medicine, University of Science and Technology of
  China (QYPY20220012 to S.T.); start-up funding from the University of Science and
  Technology of China and the\r\nChinese Academy of Sciences (GG9100007007, KY9100000026,KY9100000051,
  and KJ2070000079 to S.T.); the National Natural Science Foundation of China (31900885
  to X.L. and 31870732 to Linfeng Sun); the Natural Science Foundation of Anhui Province
  (2008085MC90 to X.L. and 2008085J15 to Linfeng Sun); the Fundamental Research Funds
  for the Central Universities (WK9100000021 to S.T. and WK9100000031 to Linfeng Sun);
  and the USTC Research Funds of the Double First-Class Initiative (YD9100002016 to
  S.T. and YD9100002004 to Linfeng Sun). Linfeng Sun is supported by an Outstanding
  Young Scholar Award from the Qiu Shi Science and Technologies Foundation and a Young
  Scholar Award from the Cyrus Tang Foundation.We thank Dr. Yang Zhao for sharing
  published materials (Center for Excellence in Molecular Plant Sciences, Chinese
  Academy of Sciences) and the Cryo-EM Center of the University of Science and Technology
  of China for the EM facility support. We are grateful to Y. Gao and all other staff
  members for their technical support on cryo-EM data collection. "
article_number: '100632'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jing
  full_name: Xia, Jing
  last_name: Xia
- first_name: Mengjuan
  full_name: Kong, Mengjuan
  last_name: Kong
- first_name: Zhisen
  full_name: Yang, Zhisen
  last_name: Yang
- first_name: Lianghanxiao
  full_name: Sun, Lianghanxiao
  last_name: Sun
- first_name: Yakun
  full_name: Peng, Yakun
  last_name: Peng
- first_name: Yanbo
  full_name: Mao, Yanbo
  last_name: Mao
- first_name: Hong
  full_name: Wei, Hong
  last_name: Wei
- first_name: Wei
  full_name: Ying, Wei
  last_name: Ying
- first_name: Yongxiao
  full_name: Gao, Yongxiao
  last_name: Gao
- first_name: Jiří
  full_name: Friml, Jiří
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
- first_name: Jianping
  full_name: Weng, Jianping
  last_name: Weng
- first_name: Xin
  full_name: Liu, Xin
  last_name: Liu
- first_name: Linfeng
  full_name: Sun, Linfeng
  last_name: Sun
- first_name: Shutang
  full_name: Tan, Shutang
  last_name: Tan
citation:
  ama: Xia J, Kong M, Yang Z, et al. Chemical inhibition of Arabidopsis PIN-FORMED
    auxin transporters by the anti-inflammatory drug naproxen. <i>Plant Communications</i>.
    2023;4(6). doi:<a href="https://doi.org/10.1016/j.xplc.2023.100632">10.1016/j.xplc.2023.100632</a>
  apa: Xia, J., Kong, M., Yang, Z., Sun, L., Peng, Y., Mao, Y., … Tan, S. (2023).
    Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
    drug naproxen. <i>Plant Communications</i>. Elsevier . <a href="https://doi.org/10.1016/j.xplc.2023.100632">https://doi.org/10.1016/j.xplc.2023.100632</a>
  chicago: Xia, Jing, Mengjuan Kong, Zhisen Yang, Lianghanxiao Sun, Yakun Peng, Yanbo
    Mao, Hong Wei, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
    by the Anti-Inflammatory Drug Naproxen.” <i>Plant Communications</i>. Elsevier
    , 2023. <a href="https://doi.org/10.1016/j.xplc.2023.100632">https://doi.org/10.1016/j.xplc.2023.100632</a>.
  ieee: J. Xia <i>et al.</i>, “Chemical inhibition of Arabidopsis PIN-FORMED auxin
    transporters by the anti-inflammatory drug naproxen,” <i>Plant Communications</i>,
    vol. 4, no. 6. Elsevier , 2023.
  ista: Xia J, Kong M, Yang Z, Sun L, Peng Y, Mao Y, Wei H, Ying W, Gao Y, Friml J,
    Weng J, Liu X, Sun L, Tan S. 2023. Chemical inhibition of Arabidopsis PIN-FORMED
    auxin transporters by the anti-inflammatory drug naproxen. Plant Communications.
    4(6), 100632.
  mla: Xia, Jing, et al. “Chemical Inhibition of Arabidopsis PIN-FORMED Auxin Transporters
    by the Anti-Inflammatory Drug Naproxen.” <i>Plant Communications</i>, vol. 4,
    no. 6, 100632, Elsevier , 2023, doi:<a href="https://doi.org/10.1016/j.xplc.2023.100632">10.1016/j.xplc.2023.100632</a>.
  short: J. Xia, M. Kong, Z. Yang, L. Sun, Y. Peng, Y. Mao, H. Wei, W. Ying, Y. Gao,
    J. Friml, J. Weng, X. Liu, L. Sun, S. Tan, Plant Communications 4 (2023).
date_created: 2023-07-12T07:32:00Z
date_published: 2023-11-13T00:00:00Z
date_updated: 2024-10-21T06:01:37Z
day: '13'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1016/j.xplc.2023.100632
external_id:
  isi:
  - '001113003000001'
  pmid:
  - '37254481'
file:
- access_level: open_access
  checksum: f8ef92af6096834f91ce38587fb1db9f
  content_type: application/pdf
  creator: dernst
  date_created: 2024-01-30T10:54:40Z
  date_updated: 2024-01-30T10:54:40Z
  file_id: '14900'
  file_name: 2023_PlantCommunications_Xia.pdf
  file_size: 1434862
  relation: main_file
  success: 1
file_date_updated: 2024-01-30T10:54:40Z
has_accepted_license: '1'
intvolume: '         4'
isi: 1
issue: '6'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: Plant Communications
publication_identifier:
  eissn:
  - 2590-3462
publication_status: published
publisher: 'Elsevier '
quality_controlled: '1'
scopus_import: '1'
status: public
title: Chemical inhibition of Arabidopsis PIN-FORMED auxin transporters by the anti-inflammatory
  drug naproxen
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2023'
...