@article{13240,
  abstract     = {Ustilago maydis is a biotrophic phytopathogenic fungus that causes corn smut disease. As a well-established model system, U. maydis is genetically fully accessible with large omics datasets available and subject to various biological questions ranging from DNA-repair, RNA-transport, and protein secretion to disease biology. For many genetic approaches, tight control of transgene regulation is important. Here we established an optimised version of the Tetracycline-ON (TetON) system for U. maydis. We demonstrate the Tetracycline concentration-dependent expression of fluorescent protein transgenes and the system’s suitability for the induced expression of the toxic protein BCL2 Associated X-1 (Bax1). The Golden Gate compatible vector system contains a native minimal promoter from the mating factor a-1 encoding gene, mfa with ten copies of the tet-regulated operator (tetO) and a codon optimised Tet-repressor (tetR*) which is translationally fused to the native transcriptional corepressor Mql1 (UMAG_05501). The metabolism-independent transcriptional regulator system is functional both, in liquid culture as well as on solid media in the presence of the inducer and can become a useful tool for toxin-antitoxin studies, identification of antifungal proteins, and to study functions of toxic gene products in Ustilago maydis.},
  author       = {Ingole, Kishor D. and Nagarajan, Nithya and Uhse, Simon and Giannini, Caterina and Djamei, Armin},
  issn         = {2673-6128},
  journal      = {Frontiers in Fungal Biology},
  publisher    = {Frontiers Media},
  title        = {{Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago maydis}},
  doi          = {10.3389/ffunb.2022.1029114},
  volume       = {3},
  year         = {2022},
}