---
_id: '13240'
abstract:
- lang: eng
  text: Ustilago maydis is a biotrophic phytopathogenic fungus that causes corn smut
    disease. As a well-established model system, U. maydis is genetically fully accessible
    with large omics datasets available and subject to various biological questions
    ranging from DNA-repair, RNA-transport, and protein secretion to disease biology.
    For many genetic approaches, tight control of transgene regulation is important.
    Here we established an optimised version of the Tetracycline-ON (TetON) system
    for U. maydis. We demonstrate the Tetracycline concentration-dependent expression
    of fluorescent protein transgenes and the system’s suitability for the induced
    expression of the toxic protein BCL2 Associated X-1 (Bax1). The Golden Gate compatible
    vector system contains a native minimal promoter from the mating factor a-1 encoding
    gene, mfa with ten copies of the tet-regulated operator (tetO) and a codon optimised
    Tet-repressor (tetR*) which is translationally fused to the native transcriptional
    corepressor Mql1 (UMAG_05501). The metabolism-independent transcriptional regulator
    system is functional both, in liquid culture as well as on solid media in the
    presence of the inducer and can become a useful tool for toxin-antitoxin studies,
    identification of antifungal proteins, and to study functions of toxic gene products
    in Ustilago maydis.
acknowledgement: "The research leading to these results received funding from the
  European Research Council under the European Union’s Seventh Framework Programme
  ERC-2013-STG (grant agreement: 335691), the Austrian Science Fund (I 3033-B22),
  the Austrian Academy of Sciences, and the Deutsche Forschungsgemeinschaft (DFG,
  German Research Foundation) under Germany's Excellence Strategy EXC-2070-390732324
  (PhenoRob) and DFG grant (DJ 64/5-1).\r\nWe would like to thank the GMI/IMBA/IMP
  core facilities for their excellent technical support. We would like to acknowledge
  Dr. Sinéad A. O’Sullivan from DZNE, University of Bonn for providing anti-GFP antibodies.
  The authors are thankful to the Excellence University of Bonn for providing infrastructure
  and instrumentation facilities at the INRES-Plant Pathology department."
article_number: '1029114'
article_processing_charge: Yes
article_type: original
author:
- first_name: Kishor D.
  full_name: Ingole, Kishor D.
  last_name: Ingole
- first_name: Nithya
  full_name: Nagarajan, Nithya
  last_name: Nagarajan
- first_name: Simon
  full_name: Uhse, Simon
  last_name: Uhse
- first_name: Caterina
  full_name: Giannini, Caterina
  id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
  last_name: Giannini
- first_name: Armin
  full_name: Djamei, Armin
  last_name: Djamei
citation:
  ama: Ingole KD, Nagarajan N, Uhse S, Giannini C, Djamei A. Tetracycline-controlled
    (TetON) gene expression system for the smut fungus Ustilago maydis. <i>Frontiers
    in Fungal Biology</i>. 2022;3. doi:<a href="https://doi.org/10.3389/ffunb.2022.1029114">10.3389/ffunb.2022.1029114</a>
  apa: Ingole, K. D., Nagarajan, N., Uhse, S., Giannini, C., &#38; Djamei, A. (2022).
    Tetracycline-controlled (TetON) gene expression system for the smut fungus Ustilago
    maydis. <i>Frontiers in Fungal Biology</i>. Frontiers Media. <a href="https://doi.org/10.3389/ffunb.2022.1029114">https://doi.org/10.3389/ffunb.2022.1029114</a>
  chicago: Ingole, Kishor D., Nithya Nagarajan, Simon Uhse, Caterina Giannini, and
    Armin Djamei. “Tetracycline-Controlled (TetON) Gene Expression System for the
    Smut Fungus Ustilago Maydis.” <i>Frontiers in Fungal Biology</i>. Frontiers Media,
    2022. <a href="https://doi.org/10.3389/ffunb.2022.1029114">https://doi.org/10.3389/ffunb.2022.1029114</a>.
  ieee: K. D. Ingole, N. Nagarajan, S. Uhse, C. Giannini, and A. Djamei, “Tetracycline-controlled
    (TetON) gene expression system for the smut fungus Ustilago maydis,” <i>Frontiers
    in Fungal Biology</i>, vol. 3. Frontiers Media, 2022.
  ista: Ingole KD, Nagarajan N, Uhse S, Giannini C, Djamei A. 2022. Tetracycline-controlled
    (TetON) gene expression system for the smut fungus Ustilago maydis. Frontiers
    in Fungal Biology. 3, 1029114.
  mla: Ingole, Kishor D., et al. “Tetracycline-Controlled (TetON) Gene Expression
    System for the Smut Fungus Ustilago Maydis.” <i>Frontiers in Fungal Biology</i>,
    vol. 3, 1029114, Frontiers Media, 2022, doi:<a href="https://doi.org/10.3389/ffunb.2022.1029114">10.3389/ffunb.2022.1029114</a>.
  short: K.D. Ingole, N. Nagarajan, S. Uhse, C. Giannini, A. Djamei, Frontiers in
    Fungal Biology 3 (2022).
date_created: 2023-07-16T22:01:12Z
date_published: 2022-10-19T00:00:00Z
date_updated: 2024-03-06T14:01:57Z
day: '19'
ddc:
- '579'
department:
- _id: JiFr
doi: 10.3389/ffunb.2022.1029114
file:
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  creator: dernst
  date_created: 2023-07-17T11:46:34Z
  date_updated: 2023-07-17T11:46:34Z
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file_date_updated: 2023-07-17T11:46:34Z
has_accepted_license: '1'
intvolume: '         3'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
publication: Frontiers in Fungal Biology
publication_identifier:
  eissn:
  - 2673-6128
publication_status: published
publisher: Frontiers Media
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tetracycline-controlled (TetON) gene expression system for the smut fungus
  Ustilago maydis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 3
year: '2022'
...