[{"doi":"10.1021/acsphyschemau.6c00026","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","chemrxivid":1,"article_type":"original","license":"https://creativecommons.org/licenses/by/4.0/","day":"04","issue":"3","author":[{"last_name":"York","full_name":"York, Emma","id":"08dde91e-8e0a-11f0-9d7d-9e8d80864f16","first_name":"Emma"},{"last_name":"Venkataraman","orcid":"0000-0002-6957-6089","full_name":"Venkataraman, Latha","id":"9ebb78a5-cc0d-11ee-8322-fae086a32caf","first_name":"Latha"}],"month":"05","status":"public","ddc":["540"],"quality_controlled":"1","publication":"ACS Physical Chemistry Au","scopus_import":"1","das_tickbox":"1","citation":{"ieee":"E. York and L. Venkataraman, “Scanning tunneling microscope-based break-junction technique - A tutorial,” <i>ACS Physical Chemistry Au</i>, vol. 6, no. 3. American Chemical Society, pp. 408–424, 2026.","ama":"York E, Venkataraman L. Scanning tunneling microscope-based break-junction technique - A tutorial. <i>ACS Physical Chemistry Au</i>. 2026;6(3):408-424. doi:<a href=\"https://doi.org/10.1021/acsphyschemau.6c00026\">10.1021/acsphyschemau.6c00026</a>","chicago":"York, Emma, and Latha Venkataraman. “Scanning Tunneling Microscope-Based Break-Junction Technique - A Tutorial.” <i>ACS Physical Chemistry Au</i>. American Chemical Society, 2026. <a href=\"https://doi.org/10.1021/acsphyschemau.6c00026\">https://doi.org/10.1021/acsphyschemau.6c00026</a>.","apa":"York, E., &#38; Venkataraman, L. (2026). Scanning tunneling microscope-based break-junction technique - A tutorial. <i>ACS Physical Chemistry Au</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsphyschemau.6c00026\">https://doi.org/10.1021/acsphyschemau.6c00026</a>","short":"E. York, L. Venkataraman, ACS Physical Chemistry Au 6 (2026) 408–424.","ista":"York E, Venkataraman L. 2026. Scanning tunneling microscope-based break-junction technique - A tutorial. ACS Physical Chemistry Au. 6(3), 408–424.","mla":"York, Emma, and Latha Venkataraman. “Scanning Tunneling Microscope-Based Break-Junction Technique - A Tutorial.” <i>ACS Physical Chemistry Au</i>, vol. 6, no. 3, American Chemical Society, 2026, pp. 408–24, doi:<a href=\"https://doi.org/10.1021/acsphyschemau.6c00026\">10.1021/acsphyschemau.6c00026</a>."},"has_accepted_license":"1","file_date_updated":"2026-06-19T06:31:16Z","acknowledgement":"We thank Michael Inkpen, Timothy Su, Masha Kamenetska, and Wanzhuo Shi for comments and Jyotisman Hazarika for data collection. This work was supported in part by the National Science Foundation (NSF-DMR 2241180) and by the Institute of Science and Technology Austria.","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"date_published":"2026-05-04T00:00:00Z","external_id":{"pmid":["42221941"],"chemrxivid":["10.26434/chemrxiv.15000474/v1"]},"corr_author":"1","oa":1,"DOAJ_listed":"1","article_processing_charge":"Yes","OA_place":"publisher","publication_status":"published","pmid":1,"fulldoi":"https://doi.org/10.1021/acsphyschemau.6c00026","oa_version":"Published Version","date_created":"2026-06-10T07:38:41Z","publication_identifier":{"eissn":["2694-2445"]},"_id":"21986","title":"Scanning tunneling microscope-based break-junction technique - A tutorial","department":[{"_id":"LaVe"}],"file":[{"date_updated":"2026-06-19T06:31:16Z","relation":"main_file","access_level":"open_access","file_name":"2026_ACSPhysChem_York.pdf","creator":"dernst","file_id":"22020","content_type":"application/pdf","checksum":"1dc16bdfb1c1cd3acde802f4350cb42a","file_size":11251172,"date_created":"2026-06-19T06:31:16Z","success":1}],"date_updated":"2026-06-22T06:19:21Z","OA_type":"gold","intvolume":"         6","page":"408-424","volume":6,"abstract":[{"lang":"eng","text":"Over the past two decades, molecular electronics has made significant progress toward discovering nanoscale analogues of conventional electronic components, largely enabled by the development of the scanning tunneling microscope-based break-junction (STM-BJ) technique. The STM-BJ technique enables precise and highly reproducible measurement of a molecule’s electronic transport properties, making it a powerful technique to explore physiochemical and electrochemical phenomena that are otherwise difficult to access. It has gained substantial popularity in the past 20 years, with experiments becoming increasingly diverse and sophisticated. Despite the wealth of literature, an accessible, practical guide to performing STM-BJ experiments and interpreting the data is largely absent. This tutorial includes a brief background into the development of STM-BJ measurements, followed by detailed explanations of instrumentation, data collection, statistical analysis, variations on standard experiments, and some troubleshooting methods. It is aimed at researchers looking to begin or improve STM-BJ studies in their laboratories, graduate students and postdoctoral researchers learning the technique, and readers seeking to critically evaluate the growing body of STM-BJ literature."}],"language":[{"iso":"eng"}],"year":"2026","type":"journal_article","PlanS_conform":"1","publisher":"American Chemical Society"},{"scopus_import":"1","citation":{"apa":"Dubini, R. C. A., Korytiaková, E., Schinkel, T., Heinrichs, P., Carell, T., &#38; Rovo, P. (2022). 1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivatives. <i>ACS Physical Chemistry Au</i>. American Chemical Society. <a href=\"https://doi.org/10.1021/acsphyschemau.1c00050\">https://doi.org/10.1021/acsphyschemau.1c00050</a>","short":"R.C.A. Dubini, E. Korytiaková, T. Schinkel, P. Heinrichs, T. Carell, P. Rovo, ACS Physical Chemistry Au 2 (2022) 237–246.","ista":"Dubini RCA, Korytiaková E, Schinkel T, Heinrichs P, Carell T, Rovo P. 2022. 1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivatives. ACS Physical Chemistry Au. 2(3), 237–246.","mla":"Dubini, Romeo C. A., et al. “1H NMR Chemical Exchange Techniques Reveal Local and Global Effects of Oxidized Cytosine Derivatives.” <i>ACS Physical Chemistry Au</i>, vol. 2, no. 3, American Chemical Society, 2022, pp. 237–46, doi:<a href=\"https://doi.org/10.1021/acsphyschemau.1c00050\">10.1021/acsphyschemau.1c00050</a>.","chicago":"Dubini, Romeo C. A., Eva Korytiaková, Thea Schinkel, Pia Heinrichs, Thomas Carell, and Petra Rovo. “1H NMR Chemical Exchange Techniques Reveal Local and Global Effects of Oxidized Cytosine Derivatives.” <i>ACS Physical Chemistry Au</i>. American Chemical Society, 2022. <a href=\"https://doi.org/10.1021/acsphyschemau.1c00050\">https://doi.org/10.1021/acsphyschemau.1c00050</a>.","ieee":"R. C. A. Dubini, E. Korytiaková, T. Schinkel, P. Heinrichs, T. Carell, and P. Rovo, “1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivatives,” <i>ACS Physical Chemistry Au</i>, vol. 2, no. 3. American Chemical Society, pp. 237–246, 2022.","ama":"Dubini RCA, Korytiaková E, Schinkel T, Heinrichs P, Carell T, Rovo P. 1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivatives. <i>ACS Physical Chemistry Au</i>. 2022;2(3):237-246. doi:<a href=\"https://doi.org/10.1021/acsphyschemau.1c00050\">10.1021/acsphyschemau.1c00050</a>"},"date_published":"2022-02-11T00:00:00Z","tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"has_accepted_license":"1","acknowledgement":"We thank Markus Müller for valued discussions and Felix Xu for assistance in the measurement of UV/vis melting profiles. This work was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) – SFB 1309-325871075, EU-ITN LightDyNAmics (ID: 765266), the ERC-AG EpiR (ID: 741912), the Center for NanoScience, the Excellence Clusters CIPSM, and the Fonds der Chemischen Industrie. Open access funding provided by Institute of Science and Technology Austria (ISTA).\r\n\r\n","file_date_updated":"2022-07-29T07:53:20Z","corr_author":"1","external_id":{"pmid":["35637781"]},"doi":"10.1021/acsphyschemau.1c00050","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","article_type":"original","day":"11","author":[{"last_name":"Dubini","first_name":"Romeo C. A.","full_name":"Dubini, Romeo C. A."},{"last_name":"Korytiaková","first_name":"Eva","full_name":"Korytiaková, Eva"},{"full_name":"Schinkel, Thea","first_name":"Thea","last_name":"Schinkel"},{"last_name":"Heinrichs","first_name":"Pia","full_name":"Heinrichs, Pia"},{"full_name":"Carell, Thomas","first_name":"Thomas","last_name":"Carell"},{"full_name":"Rovo, Petra","orcid":"0000-0001-8729-7326","id":"c316e53f-b965-11eb-b128-bb26acc59c00","first_name":"Petra","last_name":"Rovo"}],"month":"02","ddc":["540"],"status":"public","issue":"3","publication":"ACS Physical Chemistry Au","related_material":{"link":[{"url":"https://www.biorxiv.org/content/10.1101/2021.12.14.472563","relation":"earlier_version"}]},"quality_controlled":"1","intvolume":"         2","date_updated":"2025-04-15T06:53:09Z","file":[{"date_created":"2022-07-29T07:53:20Z","file_size":2351220,"success":1,"date_updated":"2022-07-29T07:53:20Z","relation":"main_file","file_name":"2022_ACSPhysChemAU_Dubini.pdf","access_level":"open_access","file_id":"11692","content_type":"application/pdf","checksum":"5ce3f907848f5c7caf77f1adfe5826c6","creator":"dernst"}],"abstract":[{"text":"5-Carboxycytosine (5caC) is a rare epigenetic modification found in nucleic acids of all domains of life. Despite its sparse genomic abundance, 5caC is presumed to play essential regulatory roles in transcription, maintenance and base-excision processes in DNA. In this work, we utilize nuclear magnetic resonance (NMR) spectroscopy to address the effects of 5caC incorporation into canonical DNA strands at multiple pH and temperature conditions. Our results demonstrate that 5caC has a pH-dependent global destabilizing and a base-pair mobility enhancing local impact on dsDNA, albeit without any detectable influence on the ground-state B-DNA structure. Measurement of hybridization thermodynamics and kinetics of 5caC-bearing DNA duplexes highlighted how acidic environment (pH 5.8 and 4.7) destabilizes the double-stranded structure by ∼10–20 kJ mol–1 at 37 °C when compared to the same sample at neutral pH. Protonation of 5caC results in a lower activation energy for the dissociation process and a higher barrier for annealing. Studies on conformational exchange on the microsecond time scale regime revealed a sharply localized base-pair motion involving exclusively the modified site and its immediate surroundings. By direct comparison with canonical and 5-formylcytosine (5fC)-edited strands, we were able to address the impact of the two most oxidized naturally occurring cytosine derivatives in the genome. These insights on 5caC’s subtle sensitivity to acidic pH contribute to the long-standing questions of its capacity as a substrate in base excision repair processes and its purpose as an independent, stable epigenetic mark.","lang":"eng"}],"page":"237-246","volume":2,"type":"journal_article","publisher":"American Chemical Society","language":[{"iso":"eng"}],"year":"2022","project":[{"_id":"B67AFEDC-15C9-11EA-A837-991A96BB2854","name":"IST Austria Open Access Fund"}],"article_processing_charge":"Yes (via OA deal)","oa":1,"pmid":1,"fulldoi":"https://doi.org/10.1021/acsphyschemau.1c00050","oa_version":"Published Version","publication_status":"published","publication_identifier":{"eissn":["2694-2445"]},"date_created":"2022-02-16T11:18:21Z","department":[{"_id":"NMR"}],"_id":"10758","title":"1H NMR chemical exchange techniques reveal local and global effects of oxidized cytosine derivatives"}]