---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19436'
abstract:
- lang: eng
  text: Dynamic DNA methylation represses transposable elements (TEs) and regulates
    gene activity, playing a pivotal role in plant development. Although substantial
    progress has been made in understanding DNA methylation reprogramming during germline
    development in Arabidopsis thaliana, whether similar mechanisms exist in other
    dicot plants remains unclear. Here, we analyzed DNA methylation levels in meiocytes,
    microspores, and pollens of Brassica Rapa using whole-genome bisulfite sequencing
    (WGBS). Global DNA methylation analysis revealed similar CHH methylation reprogramming
    compared to Arabidopsis, while distinct patterns were observed in the dynamics
    of global CG and CHG methylation in B. rapa. Differentially methylated region
    (DMR) analysis identified specifically methylated loci in the male sex cells of
    B. Rapa with a stronger tendency to target genes, similar to observations in Arabidopsis.
    Additionally, we found that the activity and genomic targeting preference of the
    small RNA-directed DNA methylation (RdDM) were altered during B. Rapa male germline
    development. A subset of long terminal repeat (LTR) TEs were activated, possibly
    due to the dynamic regulation of DNA methylation during male sexual development
    in B. Rapa. These findings provided new insights into the evolution of epigenetic
    reprogramming mechanisms in plants.
acknowledgement: We thank Prof. Ying Li of Nanjing Agricultural University for her
  help in providing seeds of K2 materials. This work was carried out with the support
  of National Natural Science Foundation of China (Grant No. 32070608).
article_number: '16'
article_processing_charge: Yes
article_type: original
author:
- first_name: Jun
  full_name: Zhang, Jun
  last_name: Zhang
- first_name: Di
  full_name: Wu, Di
  last_name: Wu
- first_name: Yating
  full_name: Zhang, Yating
  last_name: Zhang
- first_name: Xiaoqi
  full_name: Feng, Xiaoqi
  id: e0164712-22ee-11ed-b12a-d80fcdf35958
  last_name: Feng
  orcid: 0000-0002-4008-1234
- first_name: Hongbo
  full_name: Gao, Hongbo
  id: 77c2e73a-eabd-11ef-aee9-8093a2ba7a93
  last_name: Gao
citation:
  ama: Zhang J, Wu D, Zhang Y, Feng X, Gao H. DNA methylation dynamics in male germline
    development in Brassica Rapa. <i>Molecular Horticulture</i>. 2025;5. doi:<a href="https://doi.org/10.1186/s43897-024-00137-9">10.1186/s43897-024-00137-9</a>
  apa: Zhang, J., Wu, D., Zhang, Y., Feng, X., &#38; Gao, H. (2025). DNA methylation
    dynamics in male germline development in Brassica Rapa. <i>Molecular Horticulture</i>.
    Springer Nature. <a href="https://doi.org/10.1186/s43897-024-00137-9">https://doi.org/10.1186/s43897-024-00137-9</a>
  chicago: Zhang, Jun, Di Wu, Yating Zhang, Xiaoqi Feng, and Hongbo Gao. “DNA Methylation
    Dynamics in Male Germline Development in Brassica Rapa.” <i>Molecular Horticulture</i>.
    Springer Nature, 2025. <a href="https://doi.org/10.1186/s43897-024-00137-9">https://doi.org/10.1186/s43897-024-00137-9</a>.
  ieee: J. Zhang, D. Wu, Y. Zhang, X. Feng, and H. Gao, “DNA methylation dynamics
    in male germline development in Brassica Rapa,” <i>Molecular Horticulture</i>,
    vol. 5. Springer Nature, 2025.
  ista: Zhang J, Wu D, Zhang Y, Feng X, Gao H. 2025. DNA methylation dynamics in male
    germline development in Brassica Rapa. Molecular Horticulture. 5, 16.
  mla: Zhang, Jun, et al. “DNA Methylation Dynamics in Male Germline Development in
    Brassica Rapa.” <i>Molecular Horticulture</i>, vol. 5, 16, Springer Nature, 2025,
    doi:<a href="https://doi.org/10.1186/s43897-024-00137-9">10.1186/s43897-024-00137-9</a>.
  short: J. Zhang, D. Wu, Y. Zhang, X. Feng, H. Gao, Molecular Horticulture 5 (2025).
corr_author: '1'
date_created: 2025-03-23T23:01:25Z
date_published: 2025-03-04T00:00:00Z
date_updated: 2025-09-30T11:17:08Z
day: '04'
ddc:
- '580'
department:
- _id: XiFe
doi: 10.1186/s43897-024-00137-9
external_id:
  isi:
  - '001436233900001'
  pmid:
  - '40033451'
file:
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  content_type: application/pdf
  creator: dernst
  date_created: 2025-03-25T12:15:32Z
  date_updated: 2025-03-25T12:15:32Z
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  file_size: 3014980
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file_date_updated: 2025-03-25T12:15:32Z
has_accepted_license: '1'
intvolume: '         5'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: Molecular Horticulture
publication_identifier:
  eissn:
  - 2730-9401
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
status: public
title: DNA methylation dynamics in male germline development in Brassica Rapa
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 5
year: '2025'
...