---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '22297'
abstract:
- lang: eng
  text: 'Determination of the rheological properties of cells is known to require
    active measurements, which largely depend on the internalization of mechanical
    probes. Here, we circumvent this problem via the introduction of Rheological focused
    light-induced cytoplasmic streaming (Rheo-FLUCS): an active, yet probe-free approach
    that leverages light-induced flows to access mechanical changes in complex systems.
    While Rheo-FLUCS is facilitated by thermoviscous expansion phenomena rather than
    external forces, here we show equivalence in its ability to measure relative viscoelastic
    properties. Specifically, we demonstrate a phase-lag equivalence with probe-dependent
    active microrheology in a wide range of physically different, yet chemically identical
    materials. We exemplify the utility of Rheo-FLUCS in three distinctly different
    biological systems: compound-treated mouse fibroblasts (NIH-3T3), genetically
    modified human osteoblasts (U2OS) to elucidate the role of myosins in cytoplasmic
    mechanics, and early ascidian oocytes of Phallusia mammillata at fertilization
    stage. Our biological use-cases exemplify the application versatility of Rheo-FLUCS,
    which in the future may use phase information as a marker for developmental success.'
acknowledgement: The authors gratefully acknowledge help from Dr. Benjamin Seelbinder,
  Mr. Claudius George, and Mr. Falk Elsner in designing and constructing the magnetic
  needle for force-driven rheology experiments. We thank Dr. Iain Patten for valuable
  discussions on the structure of the manuscript and Mr. Ivan Saraev for graphics
  support. I.D.S. and M.K. kindly acknowledge funding from the Life grant by Volkswagen
  Foundation (Life! grant no. 92772), Deutsche Forschungsgemeinschaft (DFG, German
  Research Foundation, Germany's Excellence Strategy, 2082/1 and grant no. 515462906),
  and the Hector Foundation. I.D.S. was additionally funded by the Karlsruhe Institute
  of Technology (Excellence Strategy via the Young Investigator Group Preparation
  Program). V.R.K., S.W., and M.K. thank the ERC Starting Grant GHOSTs (grant no.
  853619).
article_number: pgag190
article_processing_charge: Yes
article_type: original
author:
- first_name: Iliya D
  full_name: Stoev, Iliya D
  last_name: Stoev
- first_name: Madison
  full_name: Bolger-Munro, Madison
  id: 516F03FA-93A3-11EA-A7C5-D6BE3DDC885E
  last_name: Bolger-Munro
  orcid: 0000-0002-8176-4824
- first_name: Antonio
  full_name: Minopoli, Antonio
  last_name: Minopoli
- first_name: Susan
  full_name: Wagner, Susan
  last_name: Wagner
- first_name: Venkat Raghavan
  full_name: Krishnaswamy, Venkat Raghavan
  last_name: Krishnaswamy
- first_name: Elena
  full_name: Erben, Elena
  last_name: Erben
- first_name: Kai
  full_name: Weißenbruch, Kai
  last_name: Weißenbruch
- first_name: Nicola
  full_name: Maghelli, Nicola
  last_name: Maghelli
- first_name: Martin
  full_name: Bastmeyer, Martin
  last_name: Bastmeyer
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Moritz
  full_name: Kreysing, Moritz
  last_name: Kreysing
biorxivid: 1
citation:
  ama: Stoev ID, Bolger-Munro M, Minopoli A, et al. Active and probe-free intracellular
    rheology via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. 2026;5(6).
    doi:<a href="https://doi.org/10.1093/pnasnexus/pgag190">10.1093/pnasnexus/pgag190</a>
  apa: Stoev, I. D., Bolger-Munro, M., Minopoli, A., Wagner, S., Krishnaswamy, V.
    R., Erben, E., … Kreysing, M. (2026). Active and probe-free intracellular rheology
    via phase-sensitive thermoviscous flows. <i>PNAS Nexus</i>. Oxford University
    Press. <a href="https://doi.org/10.1093/pnasnexus/pgag190">https://doi.org/10.1093/pnasnexus/pgag190</a>
  chicago: Stoev, Iliya D, Madison Bolger-Munro, Antonio Minopoli, Susan Wagner, Venkat
    Raghavan Krishnaswamy, Elena Erben, Kai Weißenbruch, et al. “Active and Probe-Free
    Intracellular Rheology via Phase-Sensitive Thermoviscous Flows.” <i>PNAS Nexus</i>.
    Oxford University Press, 2026. <a href="https://doi.org/10.1093/pnasnexus/pgag190">https://doi.org/10.1093/pnasnexus/pgag190</a>.
  ieee: I. D. Stoev <i>et al.</i>, “Active and probe-free intracellular rheology via
    phase-sensitive thermoviscous flows,” <i>PNAS Nexus</i>, vol. 5, no. 6. Oxford
    University Press, 2026.
  ista: Stoev ID, Bolger-Munro M, Minopoli A, Wagner S, Krishnaswamy VR, Erben E,
    Weißenbruch K, Maghelli N, Bastmeyer M, Heisenberg C-PJ, Kreysing M. 2026. Active
    and probe-free intracellular rheology via phase-sensitive thermoviscous flows.
    PNAS Nexus. 5(6), pgag190.
  mla: Stoev, Iliya D., et al. “Active and Probe-Free Intracellular Rheology via Phase-Sensitive
    Thermoviscous Flows.” <i>PNAS Nexus</i>, vol. 5, no. 6, pgag190, Oxford University
    Press, 2026, doi:<a href="https://doi.org/10.1093/pnasnexus/pgag190">10.1093/pnasnexus/pgag190</a>.
  short: I.D. Stoev, M. Bolger-Munro, A. Minopoli, S. Wagner, V.R. Krishnaswamy, E.
    Erben, K. Weißenbruch, N. Maghelli, M. Bastmeyer, C.-P.J. Heisenberg, M. Kreysing,
    PNAS Nexus 5 (2026).
das_tickbox: '1'
dataavailabilitystatement: 'All data and materials are made available on Zenodo public
  repository under Creative Commons Attribution 4.0 International License: https://doi.org/10.5281/zenodo.20322020.'
date_created: 2026-07-13T09:50:12Z
date_published: 2026-06-01T00:00:00Z
date_updated: 2026-07-13T13:14:43Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1093/pnasnexus/pgag190
external_id:
  biorxivid:
  - 10.1101/2025.04.07.647540
file:
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  checksum: bb2c89ea73da762ad6423ac299ac3533
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  date_created: 2026-07-13T13:13:56Z
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file_date_updated: 2026-07-13T13:13:56Z
fulldoi: https://doi.org/10.1093/pnasnexus/pgag190
has_accepted_license: '1'
intvolume: '         5'
issue: '6'
keyword:
- cell mechanics
- active microrheology
- noninvasiveness
- thermoviscous flows
- FLUCS
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
publication: PNAS Nexus
publication_identifier:
  eissn:
  - 2752-6542
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Active and probe-free intracellular rheology via phase-sensitive thermoviscous
  flows
tmp:
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  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 5
year: '2026'
...
---
APC_amount: 4493,27 EUR
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
PlanS_conform: '1'
_id: '20254'
abstract:
- lang: eng
  text: 'We examine population structures for their ability to maintain diversity
    in neutral evolution. We use the general framework of evolutionary graph theory
    and consider birth–death (bd) and death–birth (db) updating. The population is
    of size N. Initially all individuals represent different types. The basic question
    is: what is the time TN until one type takes over the population? This time is
    known as consensus time in computer science and as total coalescent time in evolutionary
    biology. For the complete graph, it is known that TN is quadratic in N for db
    and bd. For the cycle, we prove that TN is cubic in N for db and bd. For the star,
    we prove that TN is cubic for bd and quasilinear (N log N) for db. For the double
    star, we show that TN is quartic for bd. We derive upper and lower bounds for
    all undirected graphs for bd and db. We also show the Pareto front of graphs (of
    size N = 8) that maintain diversity the longest for bd and db. Further, we show
    that some graphs that quickly homogenize can maintain high levels of diversity
    longer than graphs that slowly homogenize. For directed graphs, we give simple
    contracting star-like structures that have superexponential time scales for maintaining
    diversity.'
acknowledgement: J.S. and K.C. were supported by the European Research Council CoG
  863818 (ForM-SMArt) and Austrian Science Fund 10.55776/COE12. J.T. was supported
  by GAČR grant 25-17377S and by Charles Univ. projects UNCE 24/SCI/008 and PRIMUS
  24/SCI/012.
article_number: pgaf252
article_processing_charge: Yes
article_type: original
arxiv: 1
author:
- first_name: David A.
  full_name: Brewster, David A.
  last_name: Brewster
- first_name: Jakub
  full_name: Svoboda, Jakub
  id: 130759D2-D7DD-11E9-87D2-DE0DE6697425
  last_name: Svoboda
  orcid: 0000-0002-1419-3267
- first_name: Dylan
  full_name: Roscow, Dylan
  last_name: Roscow
- first_name: Krishnendu
  full_name: Chatterjee, Krishnendu
  id: 2E5DCA20-F248-11E8-B48F-1D18A9856A87
  last_name: Chatterjee
  orcid: 0000-0002-4561-241X
- first_name: Josef
  full_name: Tkadlec, Josef
  id: 3F24CCC8-F248-11E8-B48F-1D18A9856A87
  last_name: Tkadlec
  orcid: 0000-0002-1097-9684
- first_name: Martin A.
  full_name: Nowak, Martin A.
  last_name: Nowak
citation:
  ama: Brewster DA, Svoboda J, Roscow D, Chatterjee K, Tkadlec J, Nowak MA. Maintaining
    diversity in structured populations. <i>PNAS Nexus</i>. 2025;4(8). doi:<a href="https://doi.org/10.1093/pnasnexus/pgaf252">10.1093/pnasnexus/pgaf252</a>
  apa: Brewster, D. A., Svoboda, J., Roscow, D., Chatterjee, K., Tkadlec, J., &#38;
    Nowak, M. A. (2025). Maintaining diversity in structured populations. <i>PNAS
    Nexus</i>. Oxford University Press. <a href="https://doi.org/10.1093/pnasnexus/pgaf252">https://doi.org/10.1093/pnasnexus/pgaf252</a>
  chicago: Brewster, David A., Jakub Svoboda, Dylan Roscow, Krishnendu Chatterjee,
    Josef Tkadlec, and Martin A. Nowak. “Maintaining Diversity in Structured Populations.”
    <i>PNAS Nexus</i>. Oxford University Press, 2025. <a href="https://doi.org/10.1093/pnasnexus/pgaf252">https://doi.org/10.1093/pnasnexus/pgaf252</a>.
  ieee: D. A. Brewster, J. Svoboda, D. Roscow, K. Chatterjee, J. Tkadlec, and M. A.
    Nowak, “Maintaining diversity in structured populations,” <i>PNAS Nexus</i>, vol.
    4, no. 8. Oxford University Press, 2025.
  ista: Brewster DA, Svoboda J, Roscow D, Chatterjee K, Tkadlec J, Nowak MA. 2025.
    Maintaining diversity in structured populations. PNAS Nexus. 4(8), pgaf252.
  mla: Brewster, David A., et al. “Maintaining Diversity in Structured Populations.”
    <i>PNAS Nexus</i>, vol. 4, no. 8, pgaf252, Oxford University Press, 2025, doi:<a
    href="https://doi.org/10.1093/pnasnexus/pgaf252">10.1093/pnasnexus/pgaf252</a>.
  short: D.A. Brewster, J. Svoboda, D. Roscow, K. Chatterjee, J. Tkadlec, M.A. Nowak,
    PNAS Nexus 4 (2025).
date_created: 2025-08-31T22:01:32Z
date_published: 2025-08-01T00:00:00Z
date_updated: 2026-06-11T09:11:17Z
day: '01'
ddc:
- '000'
department:
- _id: KrCh
doi: 10.1093/pnasnexus/pgaf252
ec_funded: 1
external_id:
  arxiv:
  - '2503.09841'
file:
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  checksum: 8a5e82c6f842e3220ec96028c9374b69
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  creator: dernst
  date_created: 2025-09-03T06:20:08Z
  date_updated: 2025-09-03T06:20:08Z
  file_id: '20280'
  file_name: 2025_PNASNexus_Brewster.pdf
  file_size: 1086419
  relation: main_file
  success: 1
file_date_updated: 2025-09-03T06:20:08Z
fulldoi: https://doi.org/10.1093/pnasnexus/pgaf252
has_accepted_license: '1'
intvolume: '         4'
issue: '8'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
project:
- _id: 0599E47C-7A3F-11EA-A408-12923DDC885E
  call_identifier: H2020
  grant_number: '863818'
  name: 'Formal Methods for Stochastic Models: Algorithms and Applications'
publication: PNAS Nexus
publication_identifier:
  eissn:
  - 2752-6542
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Maintaining diversity in structured populations
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2025'
...
---
DOAJ_listed: '1'
OA_place: publisher
OA_type: gold
_id: '19843'
abstract:
- lang: eng
  text: 'Social dilemmas are collective-action problems where individual interests
    are at odds with group interests. Such dilemmas occur frequently at all scales
    of human interactions. When dealing with collective-action problems, people often
    act reciprocally. They adjust their behavior to match the previous behavior of
    the recipient. The literature distinguishes two kinds of reciprocity. According
    to direct reciprocity, individuals react to their immediate experiences with the
    recipient. They are more likely to cooperate if the recipient previously cooperated
    with them. According to indirect reciprocity, individuals react to the recipient’s
    general behavior, irrespectively of whether or not they benefited directly. In
    practice, the two kinds of reciprocity are often intertwined; people typically
    base their decisions on both direct experiences and indirect observations. Yet
    only recently have researchers begun to explore how the two kinds of reciprocity
    interact. So far, this research only addresses a single type of social dilemma,
    the donation game, where the effects of individual behaviors are independent.
    Instead, here we allow for all pairwise social dilemmas. By applying novel techniques
    to generalize the theory of zero-determinant strategies, we establish an important
    proof of principle: In all social dilemmas, socially optimal outcomes can be sustained
    as an equilibrium, using either direct or indirect reciprocity, or arbitrary mixtures
    thereof. These results neither require games to be repeated infinitely often,
    nor that individual opinions are synchronized. In this way, we considerably generalize
    the scope of models of reciprocity, and we build further bridges between the literatures
    on direct and indirect reciprocity.'
acknowledgement: 'This work was supported by the European Research Council CoG 863818
  (ForM-SMArt) (to K.C.) and the European Research Council Starting Grant 850529:
  E-DIRECT (to C.H.).'
article_number: pgaf154
article_processing_charge: Yes
article_type: original
author:
- first_name: Valentin
  full_name: Hübner, Valentin
  id: 2c8aa207-dc7d-11ea-9b2f-f22972ecd910
  last_name: Hübner
  orcid: 0009-0001-5009-4987
- first_name: Laura
  full_name: Schmid, Laura
  id: 38B437DE-F248-11E8-B48F-1D18A9856A87
  last_name: Schmid
  orcid: 0000-0002-6978-7329
- first_name: Christian
  full_name: Hilbe, Christian
  id: 2FDF8F3C-F248-11E8-B48F-1D18A9856A87
  last_name: Hilbe
  orcid: 0000-0001-5116-955X
- first_name: Krishnendu
  full_name: Chatterjee, Krishnendu
  id: 2E5DCA20-F248-11E8-B48F-1D18A9856A87
  last_name: Chatterjee
  orcid: 0000-0002-4561-241X
citation:
  ama: Hübner V, Schmid L, Hilbe C, Chatterjee K. Stable strategies of direct and
    indirect reciprocity across all social dilemmas. <i>PNAS Nexus</i>. 2025;4(5).
    doi:<a href="https://doi.org/10.1093/pnasnexus/pgaf154">10.1093/pnasnexus/pgaf154</a>
  apa: Hübner, V., Schmid, L., Hilbe, C., &#38; Chatterjee, K. (2025). Stable strategies
    of direct and indirect reciprocity across all social dilemmas. <i>PNAS Nexus</i>.
    Oxford University Press. <a href="https://doi.org/10.1093/pnasnexus/pgaf154">https://doi.org/10.1093/pnasnexus/pgaf154</a>
  chicago: Hübner, Valentin, Laura Schmid, Christian Hilbe, and Krishnendu Chatterjee.
    “Stable Strategies of Direct and Indirect Reciprocity across All Social Dilemmas.”
    <i>PNAS Nexus</i>. Oxford University Press, 2025. <a href="https://doi.org/10.1093/pnasnexus/pgaf154">https://doi.org/10.1093/pnasnexus/pgaf154</a>.
  ieee: V. Hübner, L. Schmid, C. Hilbe, and K. Chatterjee, “Stable strategies of direct
    and indirect reciprocity across all social dilemmas,” <i>PNAS Nexus</i>, vol.
    4, no. 5. Oxford University Press, 2025.
  ista: Hübner V, Schmid L, Hilbe C, Chatterjee K. 2025. Stable strategies of direct
    and indirect reciprocity across all social dilemmas. PNAS Nexus. 4(5), pgaf154.
  mla: Hübner, Valentin, et al. “Stable Strategies of Direct and Indirect Reciprocity
    across All Social Dilemmas.” <i>PNAS Nexus</i>, vol. 4, no. 5, pgaf154, Oxford
    University Press, 2025, doi:<a href="https://doi.org/10.1093/pnasnexus/pgaf154">10.1093/pnasnexus/pgaf154</a>.
  short: V. Hübner, L. Schmid, C. Hilbe, K. Chatterjee, PNAS Nexus 4 (2025).
corr_author: '1'
date_created: 2025-06-15T22:01:30Z
date_published: 2025-05-01T00:00:00Z
date_updated: 2026-09-16T06:58:23Z
day: '01'
ddc:
- '000'
department:
- _id: KrCh
doi: 10.1093/pnasnexus/pgaf154
ec_funded: 1
external_id:
  pmid:
  - '40417077'
file:
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  checksum: efd6648db3fc3ea0cdd7155d667e5f11
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  creator: dernst
  date_created: 2025-06-23T08:09:50Z
  date_updated: 2025-06-23T08:09:50Z
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  file_name: 2025_PNASNexus_Huebner.pdf
  file_size: 2551195
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  success: 1
file_date_updated: 2025-06-23T08:09:50Z
fulldoi: https://doi.org/10.1093/pnasnexus/pgaf154
has_accepted_license: '1'
intvolume: '         4'
issue: '5'
language:
- iso: eng
month: '05'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 0599E47C-7A3F-11EA-A408-12923DDC885E
  call_identifier: H2020
  grant_number: '863818'
  name: 'Formal Methods for Stochastic Models: Algorithms and Applications'
publication: PNAS Nexus
publication_identifier:
  eissn:
  - 2752-6542
publication_status: published
publisher: Oxford University Press
quality_controlled: '1'
related_material:
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    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Stable strategies of direct and indirect reciprocity across all social dilemmas
tmp:
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  short: CC BY-NC (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 4
year: '2025'
...
---
_id: '9695'
abstract:
- lang: eng
  text: Real-world data typically contain a large number of features that are often
    heterogeneous in nature, relevance, and also units of measure. When assessing
    the similarity between data points, one can build various distance measures using
    subsets of these features. Using the fewest features but still retaining sufficient
    information about the system is crucial in many statistical learning approaches,
    particularly when data are sparse. We introduce a statistical test that can assess
    the relative information retained when using two different distance measures,
    and determine if they are equivalent, independent, or if one is more informative
    than the other. This in turn allows finding the most informative distance measure
    out of a pool of candidates. The approach is applied to find the most relevant
    policy variables for controlling the Covid-19 epidemic and to find compact yet
    informative representations of atomic structures, but its potential applications
    are wide ranging in many branches of science.
acknowledgement: "A.G., C.Z., and A.L. gratefully acknowledge support from the European
  Union’s Horizon 2020 research and innovation program (grant number 824143, MaX ’Materials
  design at the eXascale’ Centre of Excellence). The authors would like to thank M.
  Carli, D. Doimo, and I. Macocco (SISSA) for the discussions, M. Caro (Aalto University)
  for the precious help in using the TurboGap code, and D. Frenkel (University of
  Cambridge) and N. Bernstein (US Naval Research Laboratory) for useful feedback on
  the manuscript.\r\nThis work is supported in part by funds from the European Union’s
  Horizon 2020 research and innovation program (grant number 824143, MaX ’Materials
  design at the eXascale’ Centre of Excellence)."
article_number: pgac039
article_processing_charge: Yes
article_type: original
arxiv: 1
author:
- first_name: Aldo
  full_name: Glielmo, Aldo
  last_name: Glielmo
- first_name: Claudio
  full_name: Zeni, Claudio
  last_name: Zeni
- first_name: Bingqing
  full_name: Cheng, Bingqing
  id: cbe3cda4-d82c-11eb-8dc7-8ff94289fcc9
  last_name: Cheng
  orcid: 0000-0002-3584-9632
- first_name: Gabor
  full_name: Csanyi, Gabor
  last_name: Csanyi
- first_name: Alessandro
  full_name: Laio, Alessandro
  last_name: Laio
citation:
  ama: Glielmo A, Zeni C, Cheng B, Csanyi G, Laio A. Ranking the information content
    of distance measures. <i>PNAS Nexus</i>. 2022;1(2). doi:<a href="https://doi.org/10.1093/pnasnexus/pgac039">10.1093/pnasnexus/pgac039</a>
  apa: Glielmo, A., Zeni, C., Cheng, B., Csanyi, G., &#38; Laio, A. (2022). Ranking
    the information content of distance measures. <i>PNAS Nexus</i>. Oxford University
    Press. <a href="https://doi.org/10.1093/pnasnexus/pgac039">https://doi.org/10.1093/pnasnexus/pgac039</a>
  chicago: Glielmo, Aldo, Claudio Zeni, Bingqing Cheng, Gabor Csanyi, and Alessandro
    Laio. “Ranking the Information Content of Distance Measures.” <i>PNAS Nexus</i>.
    Oxford University Press, 2022. <a href="https://doi.org/10.1093/pnasnexus/pgac039">https://doi.org/10.1093/pnasnexus/pgac039</a>.
  ieee: A. Glielmo, C. Zeni, B. Cheng, G. Csanyi, and A. Laio, “Ranking the information
    content of distance measures,” <i>PNAS Nexus</i>, vol. 1, no. 2. Oxford University
    Press, 2022.
  ista: Glielmo A, Zeni C, Cheng B, Csanyi G, Laio A. 2022. Ranking the information
    content of distance measures. PNAS Nexus. 1(2), pgac039.
  mla: Glielmo, Aldo, et al. “Ranking the Information Content of Distance Measures.”
    <i>PNAS Nexus</i>, vol. 1, no. 2, pgac039, Oxford University Press, 2022, doi:<a
    href="https://doi.org/10.1093/pnasnexus/pgac039">10.1093/pnasnexus/pgac039</a>.
  short: A. Glielmo, C. Zeni, B. Cheng, G. Csanyi, A. Laio, PNAS Nexus 1 (2022).
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