@article{18168,
  abstract     = {Despite the considerable interest in the recombinant production of synthetic spider silk fibers that possess mechanical properties similar to those of native spider silks, such as the cost-effectiveness, tunability, and scalability realization, is still lacking. To address this long-standing challenge, we have constructed an artificial spider silk gene using Golden Gate assembly for the recombinant bacterial production of dragline-mimicking silk, incorporating all the essential components: the N-terminal domain, a 33-residue-long major-ampullate-spidroin-inspired segment repeated 16 times, and the C-terminal domain (N16C). This designed silk-like protein was successfully expressed in Escherichia coli, purified, and cast into films from formic acid. We produced uniformly 13C–15N-labeled N16C films and employed solid-state magic-angle spinning nuclear magnetic resonance (NMR) for characterization. Thus, we could demonstrate that our bioengineered silk-like protein self-assembles into a film where, when hydrated, the solvent-exposed layer of the rigid, β-nanocrystalline polyalanine core undergoes a transition to an α-helical structure, gaining mobility to the extent that it fully dissolves in water and transforms into a highly dynamic random coil. This hydration-induced behavior induces chain dynamics in the glycine-rich amorphous soft segments on the microsecond time scale, contributing to the elasticity of the solid material. Our findings not only reveal the presence of structurally and dynamically distinct segments within the film’s superstructure but also highlight the complexity of the self-organization responsible for the exceptional mechanical properties observed in proteins that mimic dragline silk.},
  author       = {Wu, Dongqing and Koscic, Anamaria and Schneider, Sonja and Dubini, Romeo C. A. and Rodriguez Camargo, Diana C. and Schneider, Sabine and Rovo, Petra},
  issn         = {1526-4602},
  journal      = {Biomacromolecules},
  number       = {3},
  pages        = {1759--1774},
  publisher    = {American Chemical Society},
  title        = {{Unveiling the dynamic self-assembly of a recombinant dragline-silk-mimicking protein}},
  doi          = {10.1021/acs.biomac.3c01239},
  volume       = {25},
  year         = {2024},
}

@phdthesis{18135,
  abstract     = {This thesis consists of two separate parts. In the first part we consider a dilute Fermi gas interacting through a repulsive interaction in dimensions $d=1,2,3$. Our focus is mostly on the physically most relevant dimension $d=3$ 
and the setting of a spin-polarized (equivalently spinless) gas, where the Pauli exclusion principle plays a key role. We show that, at zero temperature, the ground state energy density of the interacting spin-polarized gas differs (to leading order) from that of the free (i.e. non-interacting) gas by a term of order $a_p^d\rho^{2+2/d}$  with $a_p$ the $p$-wave scattering length of the repulsive interaction and $\rho$ the density. Further, we extend this to positive temperature and show that the pressure of an interacting spin-polarized gas differs from that of the free gas by a now temperature dependent term, again of order $a_p^d\rho^{2+2/d}$. Lastly, we consider the setting of a spin-$\frac{1}{2}$ Fermi gas in $d=3$ dimensions and show that here, as an upper bound, the ground state energy density differs from that of the free system by a term of order $a_s \rho^2$ with an error smaller than $a_s \rho^2 (a_s\rho^{1/3})^{1-\eps}$ for any $\eps > 0$, where $a_s$ is the $s$-wave scattering length of the repulsive interaction. 

These asymptotic formulas complement the similar formulas in the literature for the dilute Bose and spin-$\frac{1}{2}$ Fermi gas, where the ground state energies or pressures differ from that of the corresponding free systems by a term of order $a_s \rho^2$ in dimension $d=3$. In the spin-polarized setting, the corrections, of order $a_p^3\rho^{8/3}$ in dimension $d=3$, are thus much smaller and requires a more delicate analysis.

In the second part of the thesis we consider the Bardeen--Cooper--Schrieffer (BCS) theory of superconductivity and in particular its associated critical temperature and energy gap. We prove that the ratio of the zero-temperature energy gap and critical temperature $\Xi(T=0)/T_c$ approaches a universal constant $\pi e^{-\gamma}\approx 1.76$ in both the limit of high density in dimension $d=3$ and in the limit of weak coupling in dimensions $d=1,2$. This complements the proofs in the literature of this universal behaviour in the limit of weak coupling or low density in dimension $d=3$. Secondly, we prove that the ratio of the energy gap at positive temperature and critical temperature $\Xi(T)/T_c$ approaches a universal function of the relative temperature $T/T_c$ in the limit of weak coupling in dimensions $d=1,2,3$.},
  author       = {Lauritsen, Asbjørn Bækgaard},
  isbn         = {978-3-99078-042-8},
  issn         = {2663-337X},
  pages        = {353},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Energies of dilute Fermi gases and universalities in BCS theory}},
  doi          = {10.15479/at:ista:18135},
  year         = {2024},
}

@article{15164,
  abstract     = {Primary implant stability, which refers to the stability of the implant during the initial healing period is a crucial factor in determining the long-term success of the implant and lays the foundation for secondary implant stability achieved through osseointegration. Factors affecting primary stability include implant design, surgical technique, and patient-specific factors like bone quality and morphology. In vivo, the cyclic nature of anatomical loading puts osteosynthesis locking screws under dynamic loads, which can lead to the formation of micro cracks and defects that slowly degrade the mechanical connection between the bone and screw, thus compromising the initial stability and secondary stability of the implant. Monotonic quasi-static loading used for testing the holding capacity of implanted screws is not well suited to capture this behavior since it cannot capture the progressive deterioration of peri‑implant bone at small displacements. In order to address this issue, this study aims to determine a critical point of loss of primary implant stability in osteosynthesis locking screws under cyclic overloading by investigating the evolution of damage, dissipated energy, and permanent deformation. A custom-made test setup was used to test implanted 2.5 mm locking screws under cyclic overloading test. For each loading cycle, maximum forces and displacement were recorded as well as initial and final cycle displacements and used to calculate damage and energy dissipation evolution. The results of this study demonstrate that for axial, shear, and mixed loading significant damage and energy dissipation can be observed at approximately 20 % of the failure force. Additionally, at this load level, permanent deformations on the screw-bone interface were found to be in the range of 50 to 150 mm which promotes osseointegration and secondary implant stability. This research can assist surgeons in making informed preoperative decisions by providing a better understanding of the critical point of loss of primary implant stability, thus improving the long-term success of the implant and overall patient satisfaction.},
  author       = {Silva-Henao, Juan D. and Schober, Sophie and Pahr, Dieter H. and Reisinger, Andreas G.},
  issn         = {1873-4030},
  journal      = {Medical Engineering and Physics},
  publisher    = {Elsevier},
  title        = {{Critical loss of primary implant stability in osteosynthesis locking screws under cyclic overloading}},
  doi          = {10.1016/j.medengphy.2024.104143},
  volume       = {126},
  year         = {2024},
}

@article{14802,
  abstract     = {Frequency-stable lasers form the back bone of precision measurements in science and technology. Such lasers typically attain their stability through frequency locking to reference cavities. State-of-the-art locking performances to date had been achieved using frequency modulation based methods, complemented with active drift cancellation systems. We demonstrate an all passive, modulation-free laser-cavity locking technique (squash locking) that utilizes changes in spatial beam ellipticity for error signal generation, and a coherent polarization post-selection for noise resilience. By comparing two identically built proof-of-principle systems, we show a frequency locking instability of 5×10<jats:sup>−7</jats:sup> relative to the cavity linewidth at 10 s averaging. The results surpass the demonstrated performances of methods engineered over the last five decades, potentially enabling an advancement in the precision control of lasers, while creating avenues for bridging the performance gaps between industrial grade lasers with scientific ones due to the afforded simplicity and scalability.},
  author       = {Diorico, Fritz R and Zhutov, Artem and Hosten, Onur},
  issn         = {2334-2536},
  journal      = {Optica},
  keywords     = {Atomic and Molecular Physics, and Optics, Electronic, Optical and Magnetic Materials},
  number       = {1},
  pages        = {26--31},
  publisher    = {Optica Publishing Group},
  title        = {{Laser-cavity locking utilizing beam ellipticity: accessing the 10<sup>−7</sup> instability scale relative to cavity linewidth}},
  doi          = {10.1364/optica.507451},
  volume       = {11},
  year         = {2024},
}

@article{14931,
  abstract     = {We prove an upper bound on the ground state energy of the dilute spin-polarized Fermi gas capturing the leading correction to the kinetic energy resulting from repulsive interactions. One of the main ingredients in the proof is a rigorous implementation of the fermionic cluster expansion of Gaudin et al. (1971) [15].},
  author       = {Lauritsen, Asbjørn Bækgaard and Seiringer, Robert},
  issn         = {1096-0783},
  journal      = {Journal of Functional Analysis},
  number       = {7},
  publisher    = {Elsevier},
  title        = {{Ground state energy of the dilute spin-polarized Fermi gas: Upper bound via cluster expansion}},
  doi          = {10.1016/j.jfa.2024.110320},
  volume       = {286},
  year         = {2024},
}

@misc{15385,
  abstract     = {Relevant information about the data can be found in the 'Readme_Data.txt' file. 
A previous version of the publication can be found on BioRxiv: https://www.biorxiv.org/content/10.1101/2022.10.11.511691v4
and published in Plos Biology (2024)},
  author       = {Burnett, Laura and Koppensteiner, Peter and Symonova, Olga and Masson, Tomas and Vega Zuniga, Tomas A and Contreras, Ximena and Rülicke, Thomas and Shigemoto, Ryuichi and Novarino, Gaia and Jösch, Maximilian A},
  keywords     = {ASD, periaqueductal gray, perception, behavior, potassium channels},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Shared behavioural impairments in visual perception and place avoidance across different autism models are driven by periaqueductal grey hypoexcitability in Setd5 haploinsufficient mice}},
  doi          = {10.15479/AT:ISTA:15385},
  year         = {2024},
}

@article{14257,
  abstract     = {Mapping the complex and dense arrangement of cells and their connectivity in brain tissue demands nanoscale spatial resolution imaging. Super-resolution optical microscopy excels at visualizing specific molecules and individual cells but fails to provide tissue context. Here we developed Comprehensive Analysis of Tissues across Scales (CATS), a technology to densely map brain tissue architecture from millimeter regional to nanometer synaptic scales in diverse chemically fixed brain preparations, including rodent and human. CATS uses fixation-compatible extracellular labeling and optical imaging, including stimulated emission depletion or expansion microscopy, to comprehensively delineate cellular structures. It enables three-dimensional reconstruction of single synapses and mapping of synaptic connectivity by identification and analysis of putative synaptic cleft regions. Applying CATS to the mouse hippocampal mossy fiber circuitry, we reconstructed and quantified the synaptic input and output structure of identified neurons. We furthermore demonstrate applicability to clinically derived human tissue samples, including formalin-fixed paraffin-embedded routine diagnostic specimens, for visualizing the cellular architecture of brain tissue in health and disease.},
  author       = {Michalska, Julia M and Lyudchik, Julia and Velicky, Philipp and Korinkova, Hana and Watson, Jake and Cenameri, Alban and Sommer, Christoph M and Amberg, Nicole and Venturino, Alessandro and Roessler, Karl and Czech, Thomas and Höftberger, Romana and Siegert, Sandra and Novarino, Gaia and Jonas, Peter M and Danzl, Johann G},
  issn         = {1546-1696},
  journal      = {Nature Biotechnology},
  pages        = {1051--1064},
  publisher    = {Springer Nature},
  title        = {{Imaging brain tissue architecture across millimeter to nanometer scales}},
  doi          = {10.1038/s41587-023-01911-8},
  volume       = {42},
  year         = {2024},
}

@article{17280,
  abstract     = {Adherens junction–associated protein 1 (AJAP1) has been implicated in brain diseases; however, a pathogenic mechanism has not been identified. AJAP1 is widely expressed in neurons and binds to γ-aminobutyric acid type B receptors (GBRs), which inhibit neurotransmitter release at most synapses in the brain. Here, we show that AJAP1 is selectively expressed in dendrites and trans-synaptically recruits GBRs to presynaptic sites of neurons expressing AJAP1. We have identified several monoallelic AJAP1 variants in individuals with epilepsy and/or neurodevelopmental disorders. Specifically, we show that the variant p.(W183C) lacks binding to GBRs, resulting in the inability to recruit them. Ultrastructural analysis revealed significantly decreased presynaptic GBR levels in Ajap1−/− and Ajap1W183C/+ mice. Consequently, these mice exhibited reduced GBR-mediated presynaptic inhibition at excitatory and inhibitory synapses, along with impaired synaptic plasticity. Our study reveals that AJAP1 enables the postsynaptic neuron to regulate the level of presynaptic GBR-mediated inhibition, supporting the clinical relevance of loss-of-function AJAP1 variants.},
  author       = {Früh, Simon and Boudkkazi, Sami and Koppensteiner, Peter and Sereikaite, Vita and Chen, Li Yuan and Fernandez-Fernandez, Diego and Rem, Pascal D. and Ulrich, Daniel and Schwenk, Jochen and Chen, Ziyang and Monnier, Elodie Le and Fritzius, Thorsten and Innocenti, Sabrina M. and Besseyrias, Valérie and Trovò, Luca and Stawarski, Michal and Argilli, Emanuela and Sherr, Elliott H. and Van Bon, Bregje and Kamsteeg, Erik Jan and Iascone, Maria and Pilotta, Alba and Cutrì, Maria R. and Azamian, Mahshid S. and Hernández-García, Andrés and Lalani, Seema R. and Rosenfeld, Jill A. and Zhao, Xiaonan and Vogel, Tiphanie P. and Ona, Herda and Scott, Daryl A. and Scheiffele, Peter and Strømgaard, Kristian and Tafti, Mehdi and Gassmann, Martin and Fakler, Bernd and Shigemoto, Ryuichi and Bettler, Bernhard},
  issn         = {2375-2548},
  journal      = {Science Advances},
  number       = {28},
  publisher    = {American Association for the Advancement of Science},
  title        = {{Monoallelic de novo AJAP1 loss-of- function variants disrupt trans-synaptic control of neurotransmitter release}},
  doi          = {10.1126/sciadv.adk5462},
  volume       = {10},
  year         = {2024},
}

@phdthesis{18674,
  abstract     = {Mapping the complex and dense arrangement of cells and their connectivity in brain tissue requires volumetric imaging at nanoscale spatial resolution. While light microscopy excels at visualizing specific molecules and individual cells, achieving dense, synapse-level circuit reconstruction has not been possible with any light microscopy technique. Thus, the goal of my work was to develop image and data analysis pipelines for brain tissue visualization and reconstruction with light microscopy. To achieve dense circuit reconstruction with single-synapse resolution, I developed both conventional and deep-learning-based synapse detection algorithms, as well as connectivity analysis pipelines that integrate synapse detection with volumetric segmentation of brain tissue.},
  author       = {Lyudchik, Julia},
  isbn         = { 978-3-99078-051-0},
  issn         = {2663-337X},
  pages        = {217},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Image analysis for brain tissue reconstruction with super-resolution light microscopy}},
  doi          = {10.15479/at:ista:18674},
  year         = {2024},
}

@article{17373,
  abstract     = {Scanning Kelvin probe microscopy (SKPM) is a powerful technique for investigating the electrostatic properties of material surfaces, enabling the imaging of variations in work function, topology, surface charge density, or combinations thereof. Regardless of the underlying signal source, SKPM results in a voltage image, which is spatially distorted due to the finite size of the probe, long-range electrostatic interactions, mechanical and electrical noise, and the finite response time of the electronics. In order to recover the underlying signal, it is necessary to deconvolve the measurement with an appropriate point spread function (PSF) that accounts the aforementioned distortions, but determining this PSF is difficult. Here, we describe how such PSFs can be determined experimentally and show how they can be used to recover the underlying information of interest. We first consider the physical principles that enable SKPM and discuss how these affect the system PSF. We then show how one can experimentally measure PSFs by looking at well-defined features, and that these compare well to simulated PSFs, provided scans are performed extremely slowly and carefully. Next, we work at realistic scan speeds and show that the idealized PSFs fail to capture temporal distortions in the scan direction. While simulating PSFs for these situations would be quite challenging, we show that measuring PSFs with similar scan conditions works well. Our approach clarifies the basic principles and inherent challenges to SKPM measurements and gives practical methods to improve results.},
  author       = {Lenton, Isaac C and Pertl, Felix and Shafeek, Lubuna B and Waitukaitis, Scott R},
  issn         = {1089-7550},
  journal      = {Journal of Applied Physics},
  number       = {4},
  publisher    = {AIP Publishing},
  title        = {{Beyond the blur: Using experimentally determined point spread functions to improve scanning Kelvin probe imaging}},
  doi          = {10.1063/5.0215151},
  volume       = {136},
  year         = {2024},
}

@article{14683,
  abstract     = {Mosaic analysis with double markers (MADM) technology enables the generation of genetic mosaic tissue in mice and high-resolution phenotyping at the individual cell level. Here, we present a protocol for isolating MADM-labeled cells with high yield for downstream molecular analyses using fluorescence-activated cell sorting (FACS). We describe steps for generating MADM-labeled mice, perfusion, single-cell suspension, and debris removal. We then detail procedures for cell sorting by FACS and downstream analysis. This protocol is suitable for embryonic to adult mice.
For complete details on the use and execution of this protocol, please refer to Contreras et al. (2021).1},
  author       = {Amberg, Nicole and Cheung, Giselle T and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  keywords     = {General Immunology and Microbiology, General Biochemistry, Genetics and Molecular Biology, General Neuroscience},
  number       = {1},
  publisher    = {Elsevier},
  title        = {{Protocol for sorting cells from mouse brains labeled with mosaic analysis with double markers by flow cytometry}},
  doi          = {10.1016/j.xpro.2023.102771},
  volume       = {5},
  year         = {2024},
}

@article{17187,
  abstract     = {The generation of diverse cell types during development is fundamental to brain
functions. We outline a protocol to quantitatively assess the clonal output of individual neural progenitors using mosaic analysis with double markers (MADM) in
mice. We first describe steps to acquire and reconstruct adult MADM clones in
the superior colliculus. Then we detail analysis pipelines to determine clonal
composition and architecture. This protocol enables the buildup of quantitative
frameworks of lineage progression with precise spatial resolution in the brain.
For complete details on the use and execution of this protocol, please refer to
Cheung et al.1},
  author       = {Cheung, Giselle T and Streicher, Carmen and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {3},
  publisher    = {Elsevier},
  title        = {{Protocol for quantitative reconstruction of cell lineage using mosaic analysis with double markers in mice}},
  doi          = {10.1016/j.xpro.2024.103157},
  volume       = {5},
  year         = {2024},
}

@article{17232,
  abstract     = {The lineage relationship of clonally-related cells offers important insights into the ontogeny and cytoarchitecture of the brain in health and disease. Here, we provide a protocol to concurrently assess cell lineage relationship and cell-type identity among clonally-related cells in situ. We first describe the preparation and screening of acute brain slices containing clonally-related cells labeled using mosaic analysis with double markers (MADM). We then outline steps to collect RNA from individual cells for downstream applications and cell-type identification using RNA sequencing.
For complete details on the use and execution of this protocol, please refer to Cheung et al.
1},
  author       = {Cheung, Giselle T and Pauler, Florian and Koppensteiner, Peter and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {3},
  publisher    = {Elsevier},
  title        = {{Protocol for mapping cell lineage and cell-type identity of clonally-related cells in situ using MADM-CloneSeq}},
  doi          = {10.1016/j.xpro.2024.103168},
  volume       = {5},
  year         = {2024},
}

@article{14794,
  abstract     = {Mosaic analysis with double markers (MADM) technology enables the sparse labeling of genetically defined neurons. We present a protocol for time-lapse imaging of cortical projection neuron migration in mice using MADM. We describe steps for the isolation, culturing, and 4D imaging of neuronal dynamics in MADM-labeled brain tissue. While this protocol is compatible with other single-cell labeling methods, the MADM approach provides a genetic platform for the functional assessment of cell-autonomous candidate gene function and the relative contribution of non-cell-autonomous effects.

For complete details on the use and execution of this protocol, please refer to Hansen et al. (2022),1 Contreras et al. (2021),2 and Amberg and Hippenmeyer (2021).3},
  author       = {Hansen, Andi H and Hippenmeyer, Simon},
  issn         = {2666-1667},
  journal      = {STAR Protocols},
  number       = {1},
  publisher    = {Elsevier},
  title        = {{Time-lapse imaging of cortical projection neuron migration in mice using mosaic analysis with double markers}},
  doi          = {10.1016/j.xpro.2023.102795},
  volume       = {5},
  year         = {2024},
}

@article{15322,
  abstract     = {The tendency of materials to order in triboelectric series has prompted suggestions that contact electrification might have a single, unified underlying description. However, the possibility of “triboelectric cycles,” i.e., series that loop back onto themselves, is seemingly at odds with such a coherent description. In this work, we propose that if multiple charge carrying species are at play, both triboelectric series and cycles are possible. We show how series arise naturally if only a single charge carrier species is involved and if the driving mechanism is approach toward thermodynamic equilibrium, and simultaneously, that cycles are forbidden under such conditions. Suspecting multiple carriers might relax the situation, we affirm this is the case by explicit construction of a cycle involving two carriers, and then extend this to show how more complex cycles emerge. Our work highlights the importance of series and cycles towards determining the underlying mechanism(s) and carrier(s) in contact electrification.},
  author       = {Sobarzo Ponce, Juan Carlos A and Waitukaitis, Scott R},
  issn         = {2470-0053},
  journal      = {Physical Review E},
  number       = {3},
  publisher    = {American Physical Society},
  title        = {{Multiple charge carrier species as a possible cause for triboelectric cycles}},
  doi          = {10.1103/PhysRevE.109.L032108},
  volume       = {109},
  year         = {2024},
}

@article{14478,
  abstract     = {Entire chromosomes are typically only transmitted vertically from one generation to the next. The horizontal transfer of such chromosomes has long been considered improbable, yet gained recent support in several pathogenic fungi where it may affect the fitness or host specificity. To date, it is unknown how these transfers occur, how common they are and whether they can occur between different species. In this study, we show multiple independent instances of horizontal transfers of the same accessory chromosome between two distinct strains of the asexual entomopathogenic fungus<jats:italic>Metarhizium robertsii</jats:italic>during experimental co-infection of its insect host, the Argentine ant. Notably, only the one chromosome – but no other – was transferred from the donor to the recipient strain. The recipient strain, now harboring the accessory chromosome, exhibited a competitive advantage under certain host conditions. By phylogenetic analysis we further demonstrate that the same accessory chromosome was horizontally transferred in a natural environment between<jats:italic>M. robertsii</jats:italic>and another congeneric insect pathogen,<jats:italic>M. guizhouense</jats:italic>. Hence horizontal chromosome transfer is not limited to the observed frequent events within species during experimental infections but also occurs naturally across species. The transferred accessory chromosome contains genes that might be involved in its preferential horizontal transfer, encoding putative histones and histone-modifying enzymes, but also putative virulence factors that may support its establishment. Our study reveals that both intra- and interspecies horizontal transfer of entire chromosomes is more frequent than previously assumed, likely representing a not uncommon mechanism for gene exchange.</jats:p><jats:sec><jats:title>Significance Statement</jats:title><jats:p>The enormous success of bacterial pathogens has been attributed to their ability to exchange genetic material between one another. Similarly, in eukaryotes, horizontal transfer of genetic material allowed the spread of virulence factors across species. The horizontal transfer of whole chromosomes could be an important pathway for such exchange of genetic material, but little is known about the origin of transferable chromosomes and how frequently they are exchanged. Here, we show that the transfer of accessory chromosomes - chromosomes that are non-essential but may provide fitness benefits - is common during fungal co-infections and is even possible between distant pathogenic species, highlighting the importance of horizontal gene transfer via chromosome transfer also for the evolution and function of eukaryotic pathogens.},
  author       = {Habig, Michael and Grasse, Anna V and Müller, Judith and Stukenbrock, Eva H. and Leitner, Hanna and Cremer, Sylvia},
  issn         = {1091-6490},
  journal      = {Proceedings of the National Academy of Sciences of the United States of America},
  number       = {11},
  publisher    = {National Academy of Sciences},
  title        = {{Frequent horizontal chromosome transfer between asexual fungal insect pathogens}},
  doi          = {10.1073/pnas.2316284121},
  volume       = {121},
  year         = {2024},
}

@article{12875,
  abstract     = {The superior colliculus (SC) in the mammalian midbrain is essential for multisensory integration and is composed of a rich diversity of excitatory and inhibitory neurons and glia. However, the developmental principles directing the generation of SC cell-type diversity are not understood. Here, we pursued systematic cell lineage tracing in silico and in vivo, preserving full spatial information, using genetic mosaic analysis with double markers (MADM)-based clonal analysis with single-cell sequencing (MADM-CloneSeq). The analysis of clonally related cell lineages revealed that radial glial progenitors (RGPs) in SC are exceptionally multipotent. Individual resident RGPs have the capacity to produce all excitatory and inhibitory SC neuron types, even at the stage of terminal division. While individual clonal units show no pre-defined cellular composition, the establishment of appropriate relative proportions of distinct neuronal types occurs in a PTEN-dependent manner. Collectively, our findings provide an inaugural framework at the single-RGP/-cell level of the mammalian SC ontogeny.},
  author       = {Cheung, Giselle T and Pauler, Florian and Koppensteiner, Peter and Krausgruber, Thomas and Streicher, Carmen and Schrammel, Martin and Özgen, Natalie Y and Ivec, Alexis and Bock, Christoph and Shigemoto, Ryuichi and Hippenmeyer, Simon},
  issn         = {0896-6273},
  journal      = {Neuron},
  number       = {2},
  pages        = {230--246.e11},
  publisher    = {Elsevier},
  title        = {{Multipotent progenitors instruct ontogeny of the superior colliculus}},
  doi          = {10.1016/j.neuron.2023.11.009},
  volume       = {112},
  year         = {2024},
}

@article{14733,
  abstract     = {Redox flow batteries (RFBs) rely on the development of cheap, highly soluble, and high-energy-density electrolytes. Several candidate quinones have already been investigated in the literature as two-electron anolytes or catholytes, benefiting from fast kinetics, high tunability, and low cost. Here, an investigation of nitrogen-rich fused heteroaromatic quinones was carried out to explore avenues for electrolyte development. These quinones were synthesized and screened by using electrochemical techniques. The most promising candidate, 4,8-dioxo-4,8-dihydrobenzo[1,2-d:4,5-d′]bis([1,2,3]triazole)-1,5-diide (−0.68 V(SHE)), was tested in both an asymmetric and symmetric full-cell setup resulting in capacity fade rates of 0.35% per cycle and 0.0124% per cycle, respectively. In situ ultraviolet-visible spectroscopy (UV–Vis), nuclear magnetic resonance (NMR), and electron paramagnetic resonance (EPR) spectroscopies were used to investigate the electrochemical stability of the charged species during operation. UV–Vis spectroscopy, supported by density functional theory (DFT) modeling, reaffirmed that the two-step charging mechanism observed during battery operation consisted of two, single-electron transfers. The radical concentration during battery operation and the degree of delocalization of the unpaired electron were quantified with NMR and EPR spectroscopy.},
  author       = {Jethwa, Rajesh B and Hey, Dominic and Kerber, Rachel N. and Bond, Andrew D. and Wright, Dominic S. and Grey, Clare P.},
  issn         = {2574-0962},
  journal      = {ACS Applied Energy Materials},
  keywords     = {Electrical and Electronic Engineering, Materials Chemistry, Electrochemistry, Energy Engineering and Power Technology, Chemical Engineering (miscellaneous)},
  number       = {2},
  pages        = {414--426},
  publisher    = {American Chemical Society},
  title        = {{Exploring the landscape of heterocyclic quinones for redox flow batteries}},
  doi          = {10.1021/acsaem.3c02223},
  volume       = {7},
  year         = {2024},
}

@article{14687,
  abstract     = {The short history of research on Li-O2 batteries has seen a remarkable number of mechanistic U-turns over the years. From the initial use of carbonate electrolytes, that were then found to be entirely unsuitable, to the belief that (su)peroxide was solely responsible for degradation, before the more reactive singlet oxygen was found to form, to the hypothesis that capacity depends on a competing surface/solution mechanism before a practically exclusive solution mechanism was identified. Herein, we argue for an ever-fresh look at the reported data without bias towards supposedly established explanations. We explain how the latest findings on rate and capacity limits, as well as the origin of side reactions, are connected via the disproportionation (DISP) step in the (dis)charge mechanism. Therefrom, directions emerge for the design of electrolytes and mediators on how to suppress side reactions and to enable high rate and high reversible capacity.},
  author       = {Jethwa, Rajesh B and Mondal, Soumyadip and Pant, Bhargavi and Freunberger, Stefan Alexander},
  issn         = {1521-3773},
  journal      = {Angewandte Chemie International Edition},
  keywords     = {General Chemistry, Catalysis},
  number       = {28},
  publisher    = {Wiley},
  title        = {{To DISP or not? The far‐reaching reaction mechanisms underpinning Lithium‐air batteries}},
  doi          = {10.1002/anie.202316476},
  volume       = {63},
  year         = {2024},
}

@article{13044,
  abstract     = {Singlet oxygen (1O2) formation is now recognised as a key aspect of non-aqueous oxygen redox chemistry. For identifying 1O2, chemical trapping via 9,10-dimethylanthracene (DMA) to form the endoperoxide (DMA-O2) has become the mainstay method due to its sensitivity, selectivity, and ease of use. While DMA has been shown to be selective for 1O2, rather than forming DMA-O2 with a wide variety of potentially reactive O-containing species, false positives might hypothetically be obtained in the presence of previously overlooked species. Here, we first give unequivocal direct spectroscopic proof by the 1O2-specific near infrared (NIR) emission at 1270 nm for the previously proposed 1O2 formation pathways, which centre around superoxide disproportionation. We then show that peroxocarbonates, common intermediates in metal-O2 and metal carbonate electrochemistry, do not produce false-positive DMA-O2. Moreover, we identify a previously unreported 1O2-forming pathway through the reaction of CO2 with superoxide. Overall, we give unequivocal proof for 1O2 formation in non-aqueous oxygen redox and show that chemical trapping with DMA is a reliable method to assess 1O2 formation.},
  author       = {Mondal, Soumyadip and Jethwa, Rajesh B and Pant, Bhargavi and Hauschild, Robert and Freunberger, Stefan Alexander},
  issn         = {1364-5498},
  journal      = {Faraday Discussions},
  keywords     = {Physical and Theoretical Chemistry},
  pages        = {175--189},
  publisher    = {Royal Society of Chemistry},
  title        = {{Singlet oxygen in non-aqueous oxygen redox: Direct spectroscopic evidence for formation pathways and reliability of chemical probes}},
  doi          = {10.1039/d3fd00088e},
  volume       = {248},
  year         = {2024},
}