---
_id: '5793'
abstract:
- lang: eng
  text: The transcription coactivator, Yes-associated protein (YAP), which is a nuclear
    effector of the Hippo signaling pathway, has been shown to be a mechano-transducer.
    By using mutant fish and human 3D spheroids, we have recently demonstrated that
    YAP is also a mechano-effector. YAP functions in three-dimensional (3D) morphogenesis
    of organ and global body shape by controlling actomyosin-mediated tissue tension.
    In this chapter, we present a platform that links the findings in fish embryos
    with human cells. The protocols for analyzing tissue tension-mediated global body
    shape/organ morphogenesis in vivo and ex vivo using medaka fish embryos and in
    vitro using human cell spheroids represent useful tools for unraveling the molecular
    mechanisms by which YAP functions in regulating global body/organ morphogenesis.
alternative_title:
- MIMB
author:
- first_name: Yoichi
  full_name: Asaoka, Yoichi
  last_name: Asaoka
- first_name: Hitoshi
  full_name: Morita, Hitoshi
  last_name: Morita
- first_name: Hiroko
  full_name: Furumoto, Hiroko
  last_name: Furumoto
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Makoto
  full_name: Furutani-Seiki, Makoto
  last_name: Furutani-Seiki
citation:
  ama: 'Asaoka Y, Morita H, Furumoto H, Heisenberg C-PJ, Furutani-Seiki M. Studying
    YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In: Hergovich
    A, ed. <i>The Hippo Pathway</i>. Vol 1893. Methods in Molecular Biology. Springer;
    2019:167-181. doi:<a href="https://doi.org/10.1007/978-1-4939-8910-2_14">10.1007/978-1-4939-8910-2_14</a>'
  apa: Asaoka, Y., Morita, H., Furumoto, H., Heisenberg, C.-P. J., &#38; Furutani-Seiki,
    M. (2019). Studying YAP-mediated 3D morphogenesis using fish embryos and human
    spheroids. In A. Hergovich (Ed.), <i>The hippo pathway</i> (Vol. 1893, pp. 167–181).
    Springer. <a href="https://doi.org/10.1007/978-1-4939-8910-2_14">https://doi.org/10.1007/978-1-4939-8910-2_14</a>
  chicago: Asaoka, Yoichi, Hitoshi Morita, Hiroko Furumoto, Carl-Philipp J Heisenberg,
    and Makoto Furutani-Seiki. “Studying YAP-Mediated 3D Morphogenesis Using Fish
    Embryos and Human Spheroids.” In <i>The Hippo Pathway</i>, edited by Alexander
    Hergovich, 1893:167–81. Methods in Molecular Biology. Springer, 2019. <a href="https://doi.org/10.1007/978-1-4939-8910-2_14">https://doi.org/10.1007/978-1-4939-8910-2_14</a>.
  ieee: Y. Asaoka, H. Morita, H. Furumoto, C.-P. J. Heisenberg, and M. Furutani-Seiki,
    “Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids,”
    in <i>The hippo pathway</i>, vol. 1893, A. Hergovich, Ed. Springer, 2019, pp.
    167–181.
  ista: 'Asaoka Y, Morita H, Furumoto H, Heisenberg C-PJ, Furutani-Seiki M. 2019.Studying
    YAP-mediated 3D morphogenesis using fish embryos and human spheroids. In: The
    hippo pathway. MIMB, vol. 1893, 167–181.'
  mla: Asaoka, Yoichi, et al. “Studying YAP-Mediated 3D Morphogenesis Using Fish Embryos
    and Human Spheroids.” <i>The Hippo Pathway</i>, edited by Alexander Hergovich,
    vol. 1893, Springer, 2019, pp. 167–81, doi:<a href="https://doi.org/10.1007/978-1-4939-8910-2_14">10.1007/978-1-4939-8910-2_14</a>.
  short: Y. Asaoka, H. Morita, H. Furumoto, C.-P.J. Heisenberg, M. Furutani-Seiki,
    in:, A. Hergovich (Ed.), The Hippo Pathway, Springer, 2019, pp. 167–181.
date_created: 2019-01-06T22:59:11Z
date_published: 2019-01-01T00:00:00Z
date_updated: 2021-01-12T08:03:30Z
day: '01'
department:
- _id: CaHe
doi: 10.1007/978-1-4939-8910-2_14
editor:
- first_name: Alexander
  full_name: Hergovich, Alexander
  last_name: Hergovich
intvolume: '      1893'
language:
- iso: eng
month: '01'
oa_version: None
page: 167-181
publication: The hippo pathway
publication_identifier:
  isbn:
  - 978-1-4939-8909-6
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: 1
series_title: Methods in Molecular Biology
status: public
title: Studying YAP-mediated 3D morphogenesis using fish embryos and human spheroids
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1893
year: '2019'
...