---
OA_place: publisher
_id: '14697'
abstract:
- lang: eng
  text: "During my Ph.D. research, I managed a series of projects, each focused on
    the\r\nmechanisms underlying cell migration. My work involved an in-depth examination
    of\r\nthe complex strategies employed by neutrophils, with a specific focus on
    their ability to\r\nsynchronize spatial-temporal cues and optimize their gradient
    perception. However, it\r\nis essential to acknowledge that not all projects yielded
    successful results, as some\r\nideas were discontinued and are archived for future
    reference within this thesis.\r\nMy main project investigated how neutrophils
    decode spatial cues for precise navigation. Human neutrophils showcased distinct
    movement patterns based on source\r\ntype – linear or point-like. By combining
    single-cell tracking in 3D environments with\r\nproxy dyes, this project linked
    cell behaviors to gradient changes, revealing a stronger\r\nresponse to semi-exponential
    gradients from point sources. In addition, neutrophils\r\nexhibited oscillating
    migration speeds, using speed minima to adjust trajectories toward sources. Experiencing
    continuous concentration changes, they accelerated over\r\ntime and employed a
    \"Run and Fumble\" strategy, alternating between consistent runs\r\nand strategic
    \"tumbles\" for efficient navigation.\r\nThe project extended to the possibility
    of cells amplifying perceived gradients by\r\nenclosing their immediate surroundings,
    pushing attractants forward for enrichment\r\nwhile depleting it at the cell rear.
    Microfluidic devices were employed, and various experimental parameters configurations
    were optimized. Although significant differences\r\nin migratory efficacy were
    detected across pore sizes and device heights, quantifying\r\ngradient manipulation
    effects proved challenging.\r\nThe \"Laser-Assisted Protein Adsorption by Photobleaching\"
    (LAPAP) project was\r\npromising, as it allowed the printing of gradients. Initially
    successful with dendritic cells,\r\nwe aimed to adapt it for neutrophils. Through
    extensive experimentation with multiple\r\nparameters, we attempted to trigger
    responses from neutrophils. Despite these efforts\r\nand collaboration, the project
    failed due to practical challenges and limitations.\r\nFacing a lack of neutrophil-like
    cells at IST, we initially established the SCF-HoxB8\r\nprimary murine cell line.
    Despite their existence, their migratory behavior was largely\r\nunexplored due
    to potential limitations. Through differentiation protocol refinements we\r\nenhanced
    their migratory capabilities, though their capacity still lagged behind human\r\nneutrophils.
    Despite this, the improved migration potential of these cells pointed toward\r\ntheir
    utility for in vitro murine neutrophil migration studies."
acknowledged_ssus:
- _id: LifeSc
- _id: Bio
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Julian A
  full_name: Stopp, Julian A
  id: 489E3F00-F248-11E8-B48F-1D18A9856A87
  last_name: Stopp
citation:
  ama: 'Stopp JA. Neutrophils on the hunt : Migratory strategies employed by neutrophils
    to fulfill their effector function. 2023. doi:<a href="https://doi.org/10.15479/at:ista:14697">10.15479/at:ista:14697</a>'
  apa: 'Stopp, J. A. (2023). <i>Neutrophils on the hunt : Migratory strategies employed
    by neutrophils to fulfill their effector function</i>. Institute of Science and
    Technology Austria. <a href="https://doi.org/10.15479/at:ista:14697">https://doi.org/10.15479/at:ista:14697</a>'
  chicago: 'Stopp, Julian A. “Neutrophils on the Hunt : Migratory Strategies Employed
    by Neutrophils to Fulfill Their Effector Function.” Institute of Science and Technology
    Austria, 2023. <a href="https://doi.org/10.15479/at:ista:14697">https://doi.org/10.15479/at:ista:14697</a>.'
  ieee: 'J. A. Stopp, “Neutrophils on the hunt : Migratory strategies employed by
    neutrophils to fulfill their effector function,” Institute of Science and Technology
    Austria, 2023.'
  ista: 'Stopp JA. 2023. Neutrophils on the hunt : Migratory strategies employed by
    neutrophils to fulfill their effector function. Institute of Science and Technology
    Austria.'
  mla: 'Stopp, Julian A. <i>Neutrophils on the Hunt : Migratory Strategies Employed
    by Neutrophils to Fulfill Their Effector Function</i>. Institute of Science and
    Technology Austria, 2023, doi:<a href="https://doi.org/10.15479/at:ista:14697">10.15479/at:ista:14697</a>.'
  short: 'J.A. Stopp, Neutrophils on the Hunt : Migratory Strategies Employed by Neutrophils
    to Fulfill Their Effector Function, Institute of Science and Technology Austria,
    2023.'
corr_author: '1'
date_created: 2023-12-18T19:14:28Z
date_published: 2023-12-20T00:00:00Z
date_updated: 2026-04-07T13:57:40Z
day: '20'
ddc:
- '570'
degree_awarded: PhD
department:
- _id: GradSch
- _id: MiSi
doi: 10.15479/at:ista:14697
ec_funded: 1
file:
- access_level: open_access
  checksum: 457927165d5d556305d3086f6b83e5c7
  content_type: application/pdf
  creator: jstopp
  date_created: 2023-12-20T09:35:34Z
  date_updated: 2024-12-20T23:30:04Z
  embargo: 2024-12-20
  file_id: '14699'
  file_name: Thesis.pdf
  file_size: 51585778
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  checksum: e8d26449ac461f5e8478a62c9507506f
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: jstopp
  date_created: 2023-12-20T09:35:35Z
  date_updated: 2024-12-20T23:30:04Z
  embargo_to: open_access
  file_id: '14700'
  file_name: Thesis.docx
  file_size: 69625950
  relation: source_file
file_date_updated: 2024-12-20T23:30:04Z
has_accepted_license: '1'
language:
- iso: eng
month: '12'
oa: 1
oa_version: Published Version
page: '226'
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
publication_identifier:
  isbn:
  - 978-3-99078-038-1
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
related_material:
  record:
  - id: '14360'
    relation: part_of_dissertation
    status: public
  - id: '12272'
    relation: part_of_dissertation
    status: public
  - id: '14274'
    relation: part_of_dissertation
    status: public
  - id: '6328'
    relation: part_of_dissertation
    status: public
  - id: '7885'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
title: 'Neutrophils on the hunt : Migratory strategies employed by neutrophils to
  fulfill their effector function'
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2023'
...