[{"degree_awarded":"PhD","citation":{"ista":"Inumella S. 2025. Molecular mechanisms of microtubule reorganization in elongating root epidermal cells. Institute of Science and Technology Austria.","apa":"Inumella, S. (2025). <i>Molecular mechanisms of microtubule reorganization in elongating root epidermal cells</i>. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT-ISTA-19722\">https://doi.org/10.15479/AT-ISTA-19722</a>","mla":"Inumella, Syamala. <i>Molecular Mechanisms of Microtubule Reorganization in Elongating Root Epidermal Cells</i>. Institute of Science and Technology Austria, 2025, doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19722\">10.15479/AT-ISTA-19722</a>.","ieee":"S. Inumella, “Molecular mechanisms of microtubule reorganization in elongating root epidermal cells,” Institute of Science and Technology Austria, 2025.","short":"S. Inumella, Molecular Mechanisms of Microtubule Reorganization in Elongating Root Epidermal Cells, Institute of Science and Technology Austria, 2025.","chicago":"Inumella, Syamala. “Molecular Mechanisms of Microtubule Reorganization in Elongating Root Epidermal Cells.” Institute of Science and Technology Austria, 2025. <a href=\"https://doi.org/10.15479/AT-ISTA-19722\">https://doi.org/10.15479/AT-ISTA-19722</a>.","ama":"Inumella S. Molecular mechanisms of microtubule reorganization in elongating root epidermal cells. 2025. doi:<a href=\"https://doi.org/10.15479/AT-ISTA-19722\">10.15479/AT-ISTA-19722</a>"},"month":"05","user_id":"8b945eb4-e2f2-11eb-945a-df72226e66a9","type":"dissertation","date_created":"2025-05-23T15:21:29Z","doi":"10.15479/AT-ISTA-19722","publication_identifier":{"isbn":["978-3-99078-059-6"],"issn":["2663-337X"]},"_id":"19722","acknowledgement":"Special thanks to the Plant Facility.","department":[{"_id":"GradSch"},{"_id":"EvBe"}],"oa_version":"Published Version","oa":1,"language":[{"iso":"eng"}],"OA_place":"publisher","title":"Molecular mechanisms of microtubule reorganization in elongating root epidermal cells","file_date_updated":"2026-05-23T22:30:02Z","abstract":[{"lang":"eng","text":"As root epidermal cells progress from a phase of elongation to differentiation, their\r\ncortical microtubule (MT) arrays exhibit a transversal-to-longitudinal reorientation. The\r\nhormone cytokinin, a key regulator of root development, facilitates these cytoskeletal\r\nchanges. However, the molecular mechanisms underlying hormone-mediated MT\r\nreorientation during root development are still unknown. Here, we find that MT reorientation\r\nin root cells differs from the existing model in hypocotyl cells, as it does not rely on MT plusend rescue. We show that cytokinin facilitates MT array reorganization during cell\r\ndifferentiation by promoting katanin’s (KTN1) severing activity, and by modulating KTN1’s\r\nassociation with microtubules. Cytokinin regulates SPIRAL2 (SPR2) in a phosphorylationdependent manner, directing its localization to, and stabilization of, the new MT minus-end\r\ncreated by katanin-mediated severing at crossovers. Notably, our findings suggest that\r\ndynamic and reversible phosphorylation at S579 of SPR2 is crucial for the proper functioning\r\nof the MT severing machinery. Finally, we identify MAP65-1 and CLASP as additional targets\r\nof cytokinin-dependent phosphoregulation. Cytokinin treatment decreases MT-MAP65-1\r\nassociation in elongating cells, likely to expose MTs to KTN1-mediated severing, whereas it\r\nincreases MT-CLASP association to stabilize the growing plus-end. In this way, cytokinin drives\r\nMT reorganization during cell development by simultaneously modulating several\r\nmicrotubule-associated proteins. These results reveal key molecular players in hormonemediated cytoskeletal regulation, and highlight protein phosphorylation as a powerful tool\r\nduring this process."}],"alternative_title":["ISTA Thesis"],"page":"113","article_processing_charge":"No","supervisor":[{"full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","last_name":"Benková"}],"OA_embargo":"12","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"publisher":"Institute of Science and Technology Austria","date_updated":"2026-06-12T08:34:29Z","status":"public","publication_status":"published","file":[{"file_name":"Final Thesis_Syamala Inumella.pdf","file_id":"19757","embargo":"2026-05-23","checksum":"847ec70b2e40f50e0ddc7b8da201d52c","date_created":"2025-05-28T11:59:11Z","relation":"main_file","access_level":"open_access","creator":"sinumell","file_size":8292363,"content_type":"application/pdf","date_updated":"2026-05-23T22:30:02Z"},{"embargo_to":"open_access","file_size":7145703,"date_updated":"2026-05-23T22:30:02Z","content_type":"application/vnd.openxmlformats-officedocument.wordprocessingml.document","creator":"sinumell","access_level":"closed","relation":"source_file","checksum":"17cdffdae13a5f65bdad9c84e9e0e3bd","date_created":"2025-05-28T11:59:11Z","file_id":"19758","file_name":"Final Thesis_Syamala Inumella.docx"}],"day":"23","year":"2025","author":[{"id":"F8660870-D756-11E9-98C5-34DFE5697425","full_name":"Inumella, Syamala","orcid":"0009-0002-5890-120X","last_name":"Inumella","first_name":"Syamala"}],"date_published":"2025-05-23T00:00:00Z","ddc":["580"],"corr_author":"1","has_accepted_license":"1"}]