---
_id: '12428'
abstract:
- lang: eng
  text: The mammary gland consists of a bilayered epithelial structure with an extensively
    branched morphology. The majority of this epithelial tree is laid down during
    puberty, during which actively proliferating terminal end buds repeatedly elongate
    and bifurcate to form the basic structure of the ductal tree. Mammary ducts consist
    of a basal and luminal cell layer with a multitude of identified sub-lineages
    within both layers. The understanding of how these different cell lineages are
    cooperatively driving branching morphogenesis is a problem of crossing multiple
    scales, as this requires information on the macroscopic branched structure of
    the gland, as well as data on single-cell dynamics driving the morphogenic program.
    Here we describe a method to combine genetic lineage tracing with whole-gland
    branching analysis. Quantitative data on the global organ structure can be used
    to derive a model for mammary gland branching morphogenesis and provide a backbone
    on which the dynamics of individual cell lineages can be simulated and compared
    to lineage-tracing approaches. Eventually, these quantitative models and experiments
    allow to understand the couplings between the macroscopic shape of the mammary
    gland and the underlying single-cell dynamics driving branching morphogenesis.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Colinda L.G.J.
  full_name: Scheele, Colinda L.G.J.
  last_name: Scheele
citation:
  ama: 'Hannezo EB, Scheele CLGJ. A Guide Toward Multi-scale and Quantitative Branching
    Analysis in the Mammary Gland. In: Margadant C, ed. <i>Cell Migration in Three
    Dimensions</i>. Vol 2608. MIMB. Springer Nature; 2023:183-205. doi:<a href="https://doi.org/10.1007/978-1-0716-2887-4_12">10.1007/978-1-0716-2887-4_12</a>'
  apa: Hannezo, E. B., &#38; Scheele, C. L. G. J. (2023). A Guide Toward Multi-scale
    and Quantitative Branching Analysis in the Mammary Gland. In C. Margadant (Ed.),
    <i>Cell Migration in Three Dimensions</i> (Vol. 2608, pp. 183–205). Springer Nature.
    <a href="https://doi.org/10.1007/978-1-0716-2887-4_12">https://doi.org/10.1007/978-1-0716-2887-4_12</a>
  chicago: Hannezo, Edouard B, and Colinda L.G.J. Scheele. “A Guide Toward Multi-Scale
    and Quantitative Branching Analysis in the Mammary Gland.” In <i>Cell Migration
    in Three Dimensions</i>, edited by Coert Margadant, 2608:183–205. MIMB. Springer
    Nature, 2023. <a href="https://doi.org/10.1007/978-1-0716-2887-4_12">https://doi.org/10.1007/978-1-0716-2887-4_12</a>.
  ieee: E. B. Hannezo and C. L. G. J. Scheele, “A Guide Toward Multi-scale and Quantitative
    Branching Analysis in the Mammary Gland,” in <i>Cell Migration in Three Dimensions</i>,
    vol. 2608, C. Margadant, Ed. Springer Nature, 2023, pp. 183–205.
  ista: 'Hannezo EB, Scheele CLGJ. 2023.A Guide Toward Multi-scale and Quantitative
    Branching Analysis in the Mammary Gland. In: Cell Migration in Three Dimensions.
    Methods in Molecular Biology, vol. 2608, 183–205.'
  mla: Hannezo, Edouard B., and Colinda L. G. J. Scheele. “A Guide Toward Multi-Scale
    and Quantitative Branching Analysis in the Mammary Gland.” <i>Cell Migration in
    Three Dimensions</i>, edited by Coert Margadant, vol. 2608, Springer Nature, 2023,
    pp. 183–205, doi:<a href="https://doi.org/10.1007/978-1-0716-2887-4_12">10.1007/978-1-0716-2887-4_12</a>.
  short: E.B. Hannezo, C.L.G.J. Scheele, in:, C. Margadant (Ed.), Cell Migration in
    Three Dimensions, Springer Nature, 2023, pp. 183–205.
corr_author: '1'
date_created: 2023-01-29T23:00:58Z
date_published: 2023-01-19T00:00:00Z
date_updated: 2024-10-09T21:04:04Z
day: '19'
ddc:
- '570'
department:
- _id: EdHa
doi: 10.1007/978-1-0716-2887-4_12
editor:
- first_name: Coert
  full_name: Margadant, Coert
  last_name: Margadant
external_id:
  pmid:
  - '36653709'
file:
- access_level: open_access
  checksum: aec1b8d3ba938ddf9d8fcb777f3c38ee
  content_type: application/pdf
  creator: dernst
  date_created: 2023-02-03T10:56:39Z
  date_updated: 2023-02-03T10:56:39Z
  file_id: '12500'
  file_name: 2023_MIMB_Hannezo.pdf
  file_size: 826598
  relation: main_file
  success: 1
file_date_updated: 2023-02-03T10:56:39Z
has_accepted_license: '1'
intvolume: '      2608'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
page: 183-205
pmid: 1
publication: Cell Migration in Three Dimensions
publication_identifier:
  eisbn:
  - '9781071628874'
  eissn:
  - 1940-6029
  isbn:
  - '9781071628867'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: A Guide Toward Multi-scale and Quantitative Branching Analysis in the Mammary
  Gland
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2608
year: '2023'
...