@inbook{15361,
  abstract     = {Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented.},
  author       = {Jayasree, Aswathy and Salava, Hymavathi and Nodzynski, Tomasz and Sravankumar, Thula},
  booktitle    = {Plant Functional Genomics},
  editor       = {Maghuly, Fatemeh},
  isbn         = {9781071637777},
  issn         = {1940-6029},
  pages        = {305--313},
  publisher    = {Springer Nature},
  title        = {{Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana Protoplasts from Leaf}},
  doi          = {10.1007/978-1-0716-3778-4_21},
  volume       = {2787},
  year         = {2024},
}