---
_id: '15361'
abstract:
- lang: eng
  text: Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying
    protein-protein interactions in living cells. By fusing interacting proteins to
    fluorescent protein fragments, BiFC allows visualization of spatial localization
    patterns of protein complexes. This method has been adapted to a variety of expression
    systems in different organisms and is widely used to study protein interactions
    in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC
    assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but
    in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts
    is presented.
acknowledgement: Special thanks to Dr. Marta Zwiewka for the support. Thanks to the
  Czech Science Foundation GA 20-20860Y for financial aid and support of A.S.S., respectively.
  Thanks go to Core Facility Cellular Imaging (CELLIM), and Plant Sciences Core Facility
  of CEITEC Masaryk University is acknowledged for the technical support.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Aswathy
  full_name: Jayasree, Aswathy
  last_name: Jayasree
- first_name: Hymavathi
  full_name: Salava, Hymavathi
  last_name: Salava
- first_name: Tomasz
  full_name: Nodzynski, Tomasz
  last_name: Nodzynski
- first_name: Thula
  full_name: Sravankumar, Thula
  id: 055b7938-0b72-11ef-94eb-d14136011bb5
  last_name: Sravankumar
  orcid: 0000-0001-6925-6950
citation:
  ama: 'Jayasree A, Salava H, Nodzynski T, Sravankumar T. Protein-Protein Interactions
    Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana
    Protoplasts from Leaf. In: Maghuly F, ed. <i>Plant Functional Genomics</i>. Vol
    2787. MIMB. Springer Nature; 2024:305-313. doi:<a href="https://doi.org/10.1007/978-1-0716-3778-4_21">10.1007/978-1-0716-3778-4_21</a>'
  apa: Jayasree, A., Salava, H., Nodzynski, T., &#38; Sravankumar, T. (2024). Protein-Protein
    Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis
    thaliana Protoplasts from Leaf. In F. Maghuly (Ed.), <i>Plant Functional Genomics</i>
    (Vol. 2787, pp. 305–313). Springer Nature. <a href="https://doi.org/10.1007/978-1-0716-3778-4_21">https://doi.org/10.1007/978-1-0716-3778-4_21</a>
  chicago: Jayasree, Aswathy, Hymavathi Salava, Tomasz Nodzynski, and Thula Sravankumar.
    “Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation
    in Arabidopsis Thaliana Protoplasts from Leaf.” In <i>Plant Functional Genomics</i>,
    edited by Fatemeh Maghuly, 2787:305–13. MIMB. Springer Nature, 2024. <a href="https://doi.org/10.1007/978-1-0716-3778-4_21">https://doi.org/10.1007/978-1-0716-3778-4_21</a>.
  ieee: A. Jayasree, H. Salava, T. Nodzynski, and T. Sravankumar, “Protein-Protein
    Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis
    thaliana Protoplasts from Leaf,” in <i>Plant Functional Genomics</i>, vol. 2787,
    F. Maghuly, Ed. Springer Nature, 2024, pp. 305–313.
  ista: 'Jayasree A, Salava H, Nodzynski T, Sravankumar T. 2024.Protein-Protein Interactions
    Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana
    Protoplasts from Leaf. In: Plant Functional Genomics. Methods in Molecular Biology,
    vol. 2787, 305–313.'
  mla: Jayasree, Aswathy, et al. “Protein-Protein Interactions Visualized by Bimolecular
    Fluorescence Complementation in Arabidopsis Thaliana Protoplasts from Leaf.” <i>Plant
    Functional Genomics</i>, edited by Fatemeh Maghuly, vol. 2787, Springer Nature,
    2024, pp. 305–13, doi:<a href="https://doi.org/10.1007/978-1-0716-3778-4_21">10.1007/978-1-0716-3778-4_21</a>.
  short: A. Jayasree, H. Salava, T. Nodzynski, T. Sravankumar, in:, F. Maghuly (Ed.),
    Plant Functional Genomics, Springer Nature, 2024, pp. 305–313.
date_created: 2024-05-05T22:01:04Z
date_published: 2024-04-25T00:00:00Z
date_updated: 2024-05-06T06:39:10Z
day: '25'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-3778-4_21
editor:
- first_name: Fatemeh
  full_name: Maghuly, Fatemeh
  last_name: Maghuly
external_id:
  pmid:
  - '38656499'
intvolume: '      2787'
language:
- iso: eng
month: '04'
oa_version: None
page: 305-313
pmid: 1
publication: Plant Functional Genomics
publication_identifier:
  eissn:
  - 1940-6029
  isbn:
  - '9781071637777'
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation
  in Arabidopsis thaliana Protoplasts from Leaf
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2787
year: '2024'
...