---
OA_type: closed access
_id: '18765'
abstract:
- lang: eng
  text: Mosaic Analysis with Double Markers (MADM) represents a mouse genetic approach
    coupling differential fluorescent labeling to genetic manipulations in dividing
    cells and their lineages. MADM uniquely enables the generation and visualization
    of individual control or homozygous mutant cells in a heterozygous genetic environment.
    Among its diverse applications, MADM has been used to dissect cell-autonomous
    gene functions important for cortical development and neural development in general.
    The high cellular resolution offered by MADM also permits the analysis of transcriptomic
    changes of individual cells upon genetic manipulations. In this chapter, we describe
    an experimental protocol combining the generation and isolation of MADM-labeled
    cells with downstream single-cell RNA-sequencing technologies to probe cell-type
    specific phenotypes due to genetic mutations at single-cell resolution.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We thank all Hippenmeyer lab members for support and discussions.
  Experimental steps described were optimized with support provided by the Imaging
  & Optics Facility (IOF) and Preclinical Facility (PCF) at ISTA, Vienna BioCenter
  Core Facilities (VBCF), and Christoph Bock lab at Center for Molecular Medicine
  (CeMM). G.C. received funding from European Commission (IST plus postdoctoral fellowship).
  This work was supported by ISTA institutional funds: The Austrian Science Fund Special
  Research Programmes (FWF SFB F78 Neuro Stem Modulation) to S.H.'
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Giselle T
  full_name: Cheung, Giselle T
  id: 471195F6-F248-11E8-B48F-1D18A9856A87
  last_name: Cheung
  orcid: 0000-0001-8457-2572
- first_name: Florian
  full_name: Pauler, Florian
  id: 48EA0138-F248-11E8-B48F-1D18A9856A87
  last_name: Pauler
  orcid: 0000-0002-7462-0048
- first_name: Simon
  full_name: Hippenmeyer, Simon
  id: 37B36620-F248-11E8-B48F-1D18A9856A87
  last_name: Hippenmeyer
  orcid: 0000-0003-2279-1061
citation:
  ama: 'Cheung GT, Pauler F, Hippenmeyer S. Probing Cell-Type Specificity of Mutant
    Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM).
    In: Garcia-Marques J, Lee T, eds. <i>Lineage Tracing</i>. Vol 2886. MIMB. New
    York, NY: Springer Nature; 2025:139-151. doi:<a href="https://doi.org/10.1007/978-1-0716-4310-5_7">10.1007/978-1-0716-4310-5_7</a>'
  apa: 'Cheung, G. T., Pauler, F., &#38; Hippenmeyer, S. (2025). Probing Cell-Type
    Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis
    with Double Markers (MADM). In J. Garcia-Marques &#38; T. Lee (Eds.), <i>Lineage
    Tracing</i> (Vol. 2886, pp. 139–151). New York, NY: Springer Nature. <a href="https://doi.org/10.1007/978-1-0716-4310-5_7">https://doi.org/10.1007/978-1-0716-4310-5_7</a>'
  chicago: 'Cheung, Giselle T, Florian Pauler, and Simon Hippenmeyer. “Probing Cell-Type
    Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis
    with Double Markers (MADM).” In <i>Lineage Tracing</i>, edited by Jorge Garcia-Marques
    and Tzumin Lee, 2886:139–51. MIMB. New York, NY: Springer Nature, 2025. <a href="https://doi.org/10.1007/978-1-0716-4310-5_7">https://doi.org/10.1007/978-1-0716-4310-5_7</a>.'
  ieee: 'G. T. Cheung, F. Pauler, and S. Hippenmeyer, “Probing Cell-Type Specificity
    of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double
    Markers (MADM),” in <i>Lineage Tracing</i>, vol. 2886, J. Garcia-Marques and T.
    Lee, Eds. New York, NY: Springer Nature, 2025, pp. 139–151.'
  ista: 'Cheung GT, Pauler F, Hippenmeyer S. 2025.Probing Cell-Type Specificity of
    Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers
    (MADM). In: Lineage Tracing. Methods in Molecular Biology, vol. 2886, 139–151.'
  mla: Cheung, Giselle T., et al. “Probing Cell-Type Specificity of Mutant Phenotype
    at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM).” <i>Lineage
    Tracing</i>, edited by Jorge Garcia-Marques and Tzumin Lee, vol. 2886, Springer
    Nature, 2025, pp. 139–51, doi:<a href="https://doi.org/10.1007/978-1-0716-4310-5_7">10.1007/978-1-0716-4310-5_7</a>.
  short: G.T. Cheung, F. Pauler, S. Hippenmeyer, in:, J. Garcia-Marques, T. Lee (Eds.),
    Lineage Tracing, Springer Nature, New York, NY, 2025, pp. 139–151.
corr_author: '1'
date_created: 2025-01-07T08:36:47Z
date_published: 2025-01-03T00:00:00Z
date_updated: 2025-04-14T07:43:46Z
day: '03'
department:
- _id: SiHi
doi: 10.1007/978-1-0716-4310-5_7
ec_funded: 1
editor:
- first_name: Jorge
  full_name: Garcia-Marques, Jorge
  last_name: Garcia-Marques
- first_name: Tzumin
  full_name: Lee, Tzumin
  last_name: Lee
external_id:
  pmid:
  - '39745639'
intvolume: '      2886'
language:
- iso: eng
month: '01'
oa_version: None
page: 139-151
place: New York, NY
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '754411'
  name: ISTplus - Postdoctoral Fellowships
publication: Lineage Tracing
publication_identifier:
  eisbn:
  - '9781071643105'
  eissn:
  - 1940-6029
  isbn:
  - '9781071643099'
  issn:
  - 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Probing Cell-Type Specificity of Mutant Phenotype at Transcriptomic Level Using
  Mosaic Analysis with Double Markers (MADM)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2886
year: '2025'
...