---
_id: '8966'
abstract:
- lang: eng
  text: During development, a single cell is transformed into a highly complex organism
    through progressive cell division, specification and rearrangement. An important
    prerequisite for the emergence of patterns within the developing organism is to
    establish asymmetries at various scales, ranging from individual cells to the
    entire embryo, eventually giving rise to the different body structures. This becomes
    especially apparent during gastrulation, when the earliest major lineage restriction
    events lead to the formation of the different germ layers. Traditionally, the
    unfolding of the developmental program from symmetry breaking to germ layer formation
    has been studied by dissecting the contributions of different signaling pathways
    and cellular rearrangements in the in vivo context of intact embryos. Recent efforts,
    using the intrinsic capacity of embryonic stem cells to self-assemble and generate
    embryo-like structures de novo, have opened new avenues for understanding the
    many ways by which an embryo can be built and the influence of extrinsic factors
    therein. Here, we discuss and compare divergent and conserved strategies leading
    to germ layer formation in embryos as compared to in vitro systems, their upstream
    molecular cascades and the role of extrinsic factors in this process.
acknowledgement: We thank Nicoletta Petridou, Diana Pinheiro, Cornelia Schwayer and
  Stefania Tavano for feedback on the manuscript. Research in the Heisenberg lab is
  supported by an ERC Advanced Grant (MECSPEC 742573) to C.-P.H. A.S. is a recipient
  of a DOC Fellowship of the Austrian Academy of Science.
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Alexandra
  full_name: Schauer, Alexandra
  id: 30A536BA-F248-11E8-B48F-1D18A9856A87
  last_name: Schauer
  orcid: 0000-0001-7659-9142
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Schauer A, Heisenberg C-PJ. Reassembling gastrulation. <i>Developmental Biology</i>.
    2021;474:71-81. doi:<a href="https://doi.org/10.1016/j.ydbio.2020.12.014">10.1016/j.ydbio.2020.12.014</a>
  apa: Schauer, A., &#38; Heisenberg, C.-P. J. (2021). Reassembling gastrulation.
    <i>Developmental Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ydbio.2020.12.014">https://doi.org/10.1016/j.ydbio.2020.12.014</a>
  chicago: Schauer, Alexandra, and Carl-Philipp J Heisenberg. “Reassembling Gastrulation.”
    <i>Developmental Biology</i>. Elsevier, 2021. <a href="https://doi.org/10.1016/j.ydbio.2020.12.014">https://doi.org/10.1016/j.ydbio.2020.12.014</a>.
  ieee: A. Schauer and C.-P. J. Heisenberg, “Reassembling gastrulation,” <i>Developmental
    Biology</i>, vol. 474. Elsevier, pp. 71–81, 2021.
  ista: Schauer A, Heisenberg C-PJ. 2021. Reassembling gastrulation. Developmental
    Biology. 474, 71–81.
  mla: Schauer, Alexandra, and Carl-Philipp J. Heisenberg. “Reassembling Gastrulation.”
    <i>Developmental Biology</i>, vol. 474, Elsevier, 2021, pp. 71–81, doi:<a href="https://doi.org/10.1016/j.ydbio.2020.12.014">10.1016/j.ydbio.2020.12.014</a>.
  short: A. Schauer, C.-P.J. Heisenberg, Developmental Biology 474 (2021) 71–81.
date_created: 2020-12-22T09:53:34Z
date_published: 2021-06-01T00:00:00Z
date_updated: 2026-10-16T22:30:13Z
day: '01'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.1016/j.ydbio.2020.12.014
ec_funded: 1
external_id:
  isi:
  - '000639461800008'
  pmid:
  - '33352181'
file:
- access_level: open_access
  checksum: fa2a5731fd16ab171b029f32f031c440
  content_type: application/pdf
  creator: kschuh
  date_created: 2021-08-11T10:28:06Z
  date_updated: 2021-08-11T10:28:06Z
  file_id: '9880'
  file_name: 2021_DevBiology_Schauer.pdf
  file_size: 1440321
  relation: main_file
  success: 1
file_date_updated: 2021-08-11T10:28:06Z
fulldoi: https://doi.org/10.1016/j.ydbio.2020.12.014
has_accepted_license: '1'
intvolume: '       474'
isi: 1
keyword:
- Developmental Biology
- Cell Biology
- Molecular Biology
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: 71-81
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '742573'
  name: Interaction and feedback between cell mechanics and fate specification in
    vertebrate gastrulation
- _id: 26B1E39C-B435-11E9-9278-68D0E5697425
  grant_number: '25239'
  name: 'Mesendoderm specification in zebrafish: The role of extraembryonic tissues'
publication: Developmental Biology
publication_identifier:
  issn:
  - 0012-1606
publication_status: published
publisher: Elsevier
quality_controlled: '1'
related_material:
  record:
  - id: '12891'
    relation: dissertation_contains
    status: public
scopus_import: '1'
status: public
title: Reassembling gastrulation
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 474
year: '2021'
...
---
_id: '7545'
abstract:
- lang: eng
  text: Neuronal activity often leads to alterations in gene expression and cellular
    architecture. The nematode Caenorhabditis elegans, owing to its compact translucent
    nervous system, is a powerful system in which to study conserved aspects of the
    development and plasticity of neuronal morphology. Here we focus on one pair of
    sensory neurons, termed URX, which the worm uses to sense and avoid high levels
    of environmental oxygen. Previous studies have reported that the URX neuron pair
    has variable branched endings at its dendritic sensory tip. By controlling oxygen
    levels and analyzing mutants, we found that these microtubule-rich branched endings
    grow over time as a consequence of neuronal activity in adulthood. We also find
    that the growth of these branches correlates with an increase in cellular sensitivity
    to particular ranges of oxygen that is observable in the behavior of older worms.
    Given the strengths of C. elegans as a model organism, URX may serve as a potent
    system for uncovering genes and mechanisms involved in activity-dependent morphological
    changes in neurons and possible adaptive changes in the aging nervous system.
article_processing_charge: No
article_type: original
author:
- first_name: Jesse A.
  full_name: Cohn, Jesse A.
  last_name: Cohn
- first_name: Elizabeth R.
  full_name: Cebul, Elizabeth R.
  last_name: Cebul
- first_name: Giulio
  full_name: Valperga, Giulio
  last_name: Valperga
- first_name: Lotti
  full_name: Brose, Lotti
  last_name: Brose
- first_name: Mario
  full_name: de Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: de Bono
  orcid: 0000-0001-8347-0443
- first_name: Maxwell G.
  full_name: Heiman, Maxwell G.
  last_name: Heiman
- first_name: Jonathan T.
  full_name: Pierce, Jonathan T.
  last_name: Pierce
citation:
  ama: Cohn JA, Cebul ER, Valperga G, et al. Long-term activity drives dendritic branch
    elaboration of a C. elegans sensory neuron. <i>Developmental Biology</i>. 2020;461(1):66-74.
    doi:<a href="https://doi.org/10.1016/j.ydbio.2020.01.005">10.1016/j.ydbio.2020.01.005</a>
  apa: Cohn, J. A., Cebul, E. R., Valperga, G., Brose, L., de Bono, M., Heiman, M.
    G., &#38; Pierce, J. T. (2020). Long-term activity drives dendritic branch elaboration
    of a C. elegans sensory neuron. <i>Developmental Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ydbio.2020.01.005">https://doi.org/10.1016/j.ydbio.2020.01.005</a>
  chicago: Cohn, Jesse A., Elizabeth R. Cebul, Giulio Valperga, Lotti Brose, Mario
    de Bono, Maxwell G. Heiman, and Jonathan T. Pierce. “Long-Term Activity Drives
    Dendritic Branch Elaboration of a C. Elegans Sensory Neuron.” <i>Developmental
    Biology</i>. Elsevier, 2020. <a href="https://doi.org/10.1016/j.ydbio.2020.01.005">https://doi.org/10.1016/j.ydbio.2020.01.005</a>.
  ieee: J. A. Cohn <i>et al.</i>, “Long-term activity drives dendritic branch elaboration
    of a C. elegans sensory neuron,” <i>Developmental Biology</i>, vol. 461, no. 1.
    Elsevier, pp. 66–74, 2020.
  ista: Cohn JA, Cebul ER, Valperga G, Brose L, de Bono M, Heiman MG, Pierce JT. 2020.
    Long-term activity drives dendritic branch elaboration of a C. elegans sensory
    neuron. Developmental Biology. 461(1), 66–74.
  mla: Cohn, Jesse A., et al. “Long-Term Activity Drives Dendritic Branch Elaboration
    of a C. Elegans Sensory Neuron.” <i>Developmental Biology</i>, vol. 461, no. 1,
    Elsevier, 2020, pp. 66–74, doi:<a href="https://doi.org/10.1016/j.ydbio.2020.01.005">10.1016/j.ydbio.2020.01.005</a>.
  short: J.A. Cohn, E.R. Cebul, G. Valperga, L. Brose, M. de Bono, M.G. Heiman, J.T.
    Pierce, Developmental Biology 461 (2020) 66–74.
date_created: 2020-02-28T10:38:32Z
date_published: 2020-05-01T00:00:00Z
date_updated: 2021-01-12T08:14:06Z
day: '01'
doi: 10.1016/j.ydbio.2020.01.005
extern: '1'
fulldoi: https://doi.org/10.1016/j.ydbio.2020.01.005
intvolume: '       461'
issue: '1'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1101/685339
month: '05'
oa: 1
oa_version: Preprint
page: 66-74
publication: Developmental Biology
publication_identifier:
  issn:
  - 0012-1606
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Long-term activity drives dendritic branch elaboration of a C. elegans sensory
  neuron
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 461
year: '2020'
...
---
OA_type: free access
_id: '4141'
abstract:
- lang: eng
  text: The zyxin-related LPP protein is localized at focal adhesions and cell-cell
    contacts and is involved in the regulation of smooth muscle cell migration. A
    known interaction partner of LPP in human is the tumor suppressor protein SCRIB.
    Knocking down scrib expression c uring zebrafish embryonic development results
    in defects of convergence and extension (C&amp;amp;E) movements, which occur during
    gastrulation and mediate elongation of the anterior-posterior body axis. Mediolateral
    cell polarization underlying C&amp;amp;E is regulated by a noncanonical Writ signaling
    pathway constituting the vertebrate planar cell polarity (PCP) pathway. Here,
    we investigated the role of Lpp during early zebrafish development. We show that
    morpholino knockdown of Ipp results in defects of C&amp;amp;E, phenocopying noncanonical
    Wnt signaling mutants. Time-lapse analysis associates the defective dorsal convergence
    movements with a reduced ability to migrate along straight paths. In addition,
    expression of Lpp is significantly reduced in Wnt11 morphants and in embryos overexpressing
    Wnt11 or a dominant-negative form of Rho kinase 2, which is a downstream effector
    of Wnt11, Suggesting that Lpp expression is dependent on noncanonical Wnt signaling.
    Finally, we demonstrate that Lpp interacts with the PCP protein Scrib in zebrafish,
    and that Lpp and Scrib cooperate for the mediation of C&amp;amp;E. (C) 2008 Elsevier
    Inc. All rights reserved.
article_processing_charge: No
article_type: original
author:
- first_name: Hilke
  full_name: Vervenne, Hilke
  last_name: Vervenne
- first_name: Koen
  full_name: Crombez, Koen
  last_name: Crombez
- first_name: Kathleen
  full_name: Lambaerts, Kathleen
  last_name: Lambaerts
- first_name: Lara
  full_name: Carvalho, Lara
  last_name: Carvalho
- first_name: Mathias
  full_name: Köppen, Mathias
  last_name: Köppen
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
- first_name: Wim
  full_name: Van De Ven, Wim
  last_name: Van De Ven
- first_name: Marleen
  full_name: Petit, Marleen
  last_name: Petit
citation:
  ama: Vervenne H, Crombez K, Lambaerts K, et al. Lpp is involved in Wnt/PCP signaling
    and acts together with Scrib to mediate convergence and extension movements during
    zebrafish gastrulation. <i>Developmental Biology</i>. 2008;320(1):267-277. doi:<a
    href="https://doi.org/10.1016/j.ydbio.2008.05.529">10.1016/j.ydbio.2008.05.529</a>
  apa: Vervenne, H., Crombez, K., Lambaerts, K., Carvalho, L., Köppen, M., Heisenberg,
    C.-P. J., … Petit, M. (2008). Lpp is involved in Wnt/PCP signaling and acts together
    with Scrib to mediate convergence and extension movements during zebrafish gastrulation.
    <i>Developmental Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.ydbio.2008.05.529">https://doi.org/10.1016/j.ydbio.2008.05.529</a>
  chicago: Vervenne, Hilke, Koen Crombez, Kathleen Lambaerts, Lara Carvalho, Mathias
    Köppen, Carl-Philipp J Heisenberg, Wim Van De Ven, and Marleen Petit. “Lpp Is
    Involved in Wnt/PCP Signaling and Acts Together with Scrib to Mediate Convergence
    and Extension Movements during Zebrafish Gastrulation.” <i>Developmental Biology</i>.
    Elsevier, 2008. <a href="https://doi.org/10.1016/j.ydbio.2008.05.529">https://doi.org/10.1016/j.ydbio.2008.05.529</a>.
  ieee: H. Vervenne <i>et al.</i>, “Lpp is involved in Wnt/PCP signaling and acts
    together with Scrib to mediate convergence and extension movements during zebrafish
    gastrulation,” <i>Developmental Biology</i>, vol. 320, no. 1. Elsevier, pp. 267–277,
    2008.
  ista: Vervenne H, Crombez K, Lambaerts K, Carvalho L, Köppen M, Heisenberg C-PJ,
    Van De Ven W, Petit M. 2008. Lpp is involved in Wnt/PCP signaling and acts together
    with Scrib to mediate convergence and extension movements during zebrafish gastrulation.
    Developmental Biology. 320(1), 267–277.
  mla: Vervenne, Hilke, et al. “Lpp Is Involved in Wnt/PCP Signaling and Acts Together
    with Scrib to Mediate Convergence and Extension Movements during Zebrafish Gastrulation.”
    <i>Developmental Biology</i>, vol. 320, no. 1, Elsevier, 2008, pp. 267–77, doi:<a
    href="https://doi.org/10.1016/j.ydbio.2008.05.529">10.1016/j.ydbio.2008.05.529</a>.
  short: H. Vervenne, K. Crombez, K. Lambaerts, L. Carvalho, M. Köppen, C.-P.J. Heisenberg,
    W. Van De Ven, M. Petit, Developmental Biology 320 (2008) 267–277.
date_created: 2018-12-11T12:07:11Z
date_published: 2008-08-01T00:00:00Z
date_updated: 2026-05-29T08:31:06Z
day: '01'
doi: 10.1016/j.ydbio.2008.05.529
extern: '1'
external_id:
  pmid:
  - '18582857'
fulldoi: https://doi.org/10.1016/j.ydbio.2008.05.529
intvolume: '       320'
issue: '1'
language:
- iso: eng
month: '08'
oa_version: None
page: 267 - 277
pmid: 1
publication: Developmental Biology
publication_identifier:
  issn:
  - 0012-1606
publication_status: published
publisher: Elsevier
publist_id: '1978'
status: public
title: Lpp is involved in Wnt/PCP signaling and acts together with Scrib to mediate
  convergence and extension movements during zebrafish gastrulation
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 320
year: '2008'
...