[{"fulldoi":"https://doi.org/10.1016/S0021-9258(18)42219-3","oa_version":"Published Version","publication_identifier":{"issn":["0021-9258"]},"pmid":1,"publist_id":"4366","oa":1,"language":[{"iso":"eng"}],"date_updated":"2022-03-17T15:08:29Z","author":[{"full_name":"Abe, Takaaki","first_name":"Takaaki","last_name":"Abe"},{"last_name":"Sugihara","first_name":"Hidemitsu","full_name":"Sugihara, Hidemitsu"},{"full_name":"Nawa, Hiroyuki","last_name":"Nawa","first_name":"Hiroyuki"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","first_name":"Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Noboru","last_name":"Mizuno","full_name":"Mizuno, Noboru"},{"last_name":"Nakanishi","first_name":"Shigetada","full_name":"Nakanishi, Shigetada"}],"citation":{"mla":"Abe, Takaaki, et al. “Molecular Characterization of a Novel Metabotropic Glutamate Receptor MGluR5 Coupled to Inositol Phosphate/Ca2+ Signal Transduction.” <i>Journal of Biological Chemistry</i>, vol. 267, no. 19, American Society for Biochemistry and Molecular Biology, 1992, pp. 13361–68, doi:<a href=\"https://doi.org/10.1016/S0021-9258(18)42219-3\">10.1016/S0021-9258(18)42219-3</a>.","short":"T. Abe, H. Sugihara, H. Nawa, R. Shigemoto, N. Mizuno, S. Nakanishi, Journal of Biological Chemistry 267 (1992) 13361–13368.","chicago":"Abe, Takaaki, Hidemitsu Sugihara, Hiroyuki Nawa, Ryuichi Shigemoto, Noboru Mizuno, and Shigetada Nakanishi. “Molecular Characterization of a Novel Metabotropic Glutamate Receptor MGluR5 Coupled to Inositol Phosphate/Ca2+ Signal Transduction.” <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology, 1992. <a href=\"https://doi.org/10.1016/S0021-9258(18)42219-3\">https://doi.org/10.1016/S0021-9258(18)42219-3</a>.","ista":"Abe T, Sugihara H, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. 1992. Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. Journal of Biological Chemistry. 267(19), 13361–13368.","ama":"Abe T, Sugihara H, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. <i>Journal of Biological Chemistry</i>. 1992;267(19):13361-13368. doi:<a href=\"https://doi.org/10.1016/S0021-9258(18)42219-3\">10.1016/S0021-9258(18)42219-3</a>","apa":"Abe, T., Sugihara, H., Nawa, H., Shigemoto, R., Mizuno, N., &#38; Nakanishi, S. (1992). Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1016/S0021-9258(18)42219-3\">https://doi.org/10.1016/S0021-9258(18)42219-3</a>","ieee":"T. Abe, H. Sugihara, H. Nawa, R. Shigemoto, N. Mizuno, and S. Nakanishi, “Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction,” <i>Journal of Biological Chemistry</i>, vol. 267, no. 19. American Society for Biochemistry and Molecular Biology, pp. 13361–13368, 1992."},"abstract":[{"text":"A cDNA clone for a new metabotropic glutamate receptor, mGluR5, was isolated through polymerase chain reaction-mediated DNA amplification by using primer sequences conserved among the metabotropic glutamate receptor (mGluR) family and by the subsequent screening of a rat brain cDNA library. The cloned receptor consists of 1171 amino acid residues and exhibits a structural architecture common to the mGluR family, possessing a large extracellular domain preceding the seven putative membrane-spanning segments. mGluR5 shows the highest sequence similarity to mGluR1 among the mGluR members and is coupled to the stimulation of phosphatidylinositol hydrolysis/ Ca2+ signal transduction in Chinese hamster ovary cells transfected with the cloned cDNA. This receptor also resembles mGluR1 in its agonist selectivity and antagonist responses; the potency rank order of agonists for mGluR5 was determined to be quisqualate &gt; L-glutamate ≥ ibotenate &gt; trans-1-aminocyclopentane-1,3-dicarboxylate. Blot and in situ hybridization analyses indicated that mGluR5 mRNA is widely distributed in neuronal cells of the central nervous system and is expressed differently from mGluR1 mRNA in many brain regions. This investigation thus demonstrates that there is an additional mGluR subtype which closely resembles mGluR1 in its signal transduction and pharmacological properties and is expressed in specialized neuronal cells in the central nervous system.","lang":"eng"}],"year":"1992","acknowledgement":"We are grateful to Seiji Ito for help of Ca2+ measurements and Akira Uesugi for photographic assistance.","scopus_import":"1","doi":"10.1016/S0021-9258(18)42219-3","volume":267,"article_type":"original","issue":"19","extern":"1","main_file_link":[{"open_access":"1","url":"https://www.sciencedirect.com/science/article/pii/S0021925818422193"}],"article_processing_charge":"No","month":"07","quality_controlled":"1","type":"journal_article","title":"Molecular characterization of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+ signal transduction","status":"public","intvolume":"       267","external_id":{"pmid":["1320017"]},"_id":"2533","publication":"Journal of Biological Chemistry","day":"05","page":"13361 - 13368","publisher":"American Society for Biochemistry and Molecular Biology","publication_status":"published","date_published":"1992-07-05T00:00:00Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_created":"2018-12-11T11:58:14Z"},{"publication":"Journal of Biological Chemistry","_id":"2480","day":"15","page":"623 - 628","publication_status":"published","publisher":"American Society for Biochemistry and Molecular Biology","date_created":"2018-12-11T11:57:55Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"1990-01-15T00:00:00Z","month":"01","quality_controlled":"1","title":"Cloning and expression of a rat neuromedin K receptor cDNA","type":"journal_article","status":"public","intvolume":"       265","external_id":{"pmid":["2153106 "]},"citation":{"ista":"Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S. 1990. Cloning and expression of a rat neuromedin K receptor cDNA. Journal of Biological Chemistry. 265(2), 623–628.","ama":"Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S. Cloning and expression of a rat neuromedin K receptor cDNA. <i>Journal of Biological Chemistry</i>. 1990;265(2):623-628. doi:<a href=\"https://doi.org/10.1016/s0021-9258(19)40095-1 \">10.1016/s0021-9258(19)40095-1 </a>","apa":"Shigemoto, R., Yokota, Y., Tsuchida, K., &#38; Nakanishi, S. (1990). Cloning and expression of a rat neuromedin K receptor cDNA. <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1016/s0021-9258(19)40095-1 \">https://doi.org/10.1016/s0021-9258(19)40095-1 </a>","ieee":"R. Shigemoto, Y. Yokota, K. Tsuchida, and S. Nakanishi, “Cloning and expression of a rat neuromedin K receptor cDNA,” <i>Journal of Biological Chemistry</i>, vol. 265, no. 2. American Society for Biochemistry and Molecular Biology, pp. 623–628, 1990.","mla":"Shigemoto, Ryuichi, et al. “Cloning and Expression of a Rat Neuromedin K Receptor CDNA.” <i>Journal of Biological Chemistry</i>, vol. 265, no. 2, American Society for Biochemistry and Molecular Biology, 1990, pp. 623–28, doi:<a href=\"https://doi.org/10.1016/s0021-9258(19)40095-1 \">10.1016/s0021-9258(19)40095-1 </a>.","short":"R. Shigemoto, Y. Yokota, K. Tsuchida, S. Nakanishi, Journal of Biological Chemistry 265 (1990) 623–628.","chicago":"Shigemoto, Ryuichi, Yoshifumi Yokota, Kunihiro Tsuchida, and Shigetada Nakanishi. “Cloning and Expression of a Rat Neuromedin K Receptor CDNA.” <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology, 1990. <a href=\"https://doi.org/10.1016/s0021-9258(19)40095-1 \">https://doi.org/10.1016/s0021-9258(19)40095-1 </a>."},"scopus_import":"1","abstract":[{"text":"Functional cDNA clones for rat neuromedin K receptor were isolated from a rat brain cDNA library by cross-hybridization with the bovine substance K recepor cDNA. Injection of the mRNA synthesized in vitro from the cloned cDNA into Xenopus oocytes elicited electrophysiological responses to tachykinins, with the most potent sensitivity being to neuromedin K. Ligand-binding displacement in membranes of mammalian COS cells transfected with the cDNA indicated the rank order of affinity of the receptor to tachykinins; neuromedin K &gt; substance K &gt; substance P. The hybridization analysis showed that the neuromedin K receptor mRNA is expressed in both the brain and the peripheral tissues at different levels. The rat neuromedin K receptor consists of 452 amino acid residues and belongs to the family of G protein-coupled receptors, which are thought to have seven transmembrane domains. The sequence comparison of the rat neuromedin K, substance P, and substance K receptors revealed that these receptors are highly conserved in the seven transmembrane domains and the cytoplasmic sides of the receptors. They also show some structural characteristics, including the common presence of histidine residues in transmembrane segments V and VI and the difference in the numbers and distributions of serine and threonine residues as possible phosphorylation sites in the cytoplasmic regions. This paper thus presents the first comprehensive analysis of the molecular nature of the multiple peptide receptors that exhibit similar but pharmacologically distinguishable activities.","lang":"eng"}],"acknowledgement":"This work was supported in part by research grants from the Ministry Education, Science and Culture of Japan; the Institute of Physical and Chemical Research; and the Science and Technology Agency of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 USC. Section 1734 solely to indicate this fact.","year":"1990","doi":"10.1016/s0021-9258(19)40095-1 ","volume":265,"article_type":"original","extern":"1","issue":"2","article_processing_charge":"No","main_file_link":[{"url":"https://www.sciencedirect.com/science/article/pii/S0021925819400951","open_access":"1"}],"oa_version":"Published Version","fulldoi":"https://doi.org/10.1016/s0021-9258(19)40095-1 ","publication_identifier":{"issn":["0021-9258"],"eissn":["1083-351X"]},"pmid":1,"oa":1,"publist_id":"4421","language":[{"iso":"eng"}],"author":[{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","first_name":"Ryuichi"},{"last_name":"Yokota","first_name":"Yoshifumi","full_name":"Yokota, Yoshifumi"},{"full_name":"Tsuchida, Kunihiro","first_name":"Kunihiro","last_name":"Tsuchida"},{"full_name":"Nakanishi, Shigetada","last_name":"Nakanishi","first_name":"Shigetada"}],"date_updated":"2022-02-24T11:07:05Z"},{"oa":1,"publist_id":"4374","pmid":1,"date_updated":"2025-06-26T09:18:02Z","author":[{"full_name":"Yokota, Yoshifumi","last_name":"Yokota","first_name":"Yoshifumi"},{"full_name":"Sasai, Yoshiki","first_name":"Yoshiki","last_name":"Sasai"},{"full_name":"Tanaka, Kohichi","first_name":"Kohichi","last_name":"Tanaka"},{"last_name":"Fujiwara","first_name":"Tsutomu","full_name":"Fujiwara, Tsutomu"},{"first_name":"Kunihiro","last_name":"Tsuchida","full_name":"Tsuchida, Kunihiro"},{"id":"499F3ABC-F248-11E8-B48F-1D18A9856A87","last_name":"Shigemoto","first_name":"Ryuichi","full_name":"Shigemoto, Ryuichi","orcid":"0000-0001-8761-9444"},{"first_name":"Akira","last_name":"Kakizuka","full_name":"Kakizuka, Akira"},{"full_name":"Ohkubo, Hiroaki","last_name":"Ohkubo","first_name":"Hiroaki"},{"full_name":"Nakanishi, Shigetada","last_name":"Nakanishi","first_name":"Shigetada"}],"language":[{"iso":"eng"}],"oa_version":"Published Version","fulldoi":"https://doi.org/10.1016/S0021-9258(19)84619-7","publication_identifier":{"eissn":["1083-351X"],"issn":["0021-9258"]},"article_type":"original","volume":264,"main_file_link":[{"url":"https://doi.org/10.1016/S0021-9258(19)84619-7","open_access":"1"}],"article_processing_charge":"No","issue":"30","extern":"1","citation":{"apa":"Yokota, Y., Sasai, Y., Tanaka, K., Fujiwara, T., Tsuchida, K., Shigemoto, R., … Nakanishi, S. (1989). Molecular characterization of a functional cDNA for rat substance P receptor. <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href=\"https://doi.org/10.1016/S0021-9258(19)84619-7\">https://doi.org/10.1016/S0021-9258(19)84619-7</a>","ama":"Yokota Y, Sasai Y, Tanaka K, et al. Molecular characterization of a functional cDNA for rat substance P receptor. <i>Journal of Biological Chemistry</i>. 1989;264(30):17649-17652. doi:<a href=\"https://doi.org/10.1016/S0021-9258(19)84619-7\">10.1016/S0021-9258(19)84619-7</a>","ista":"Yokota Y, Sasai Y, Tanaka K, Fujiwara T, Tsuchida K, Shigemoto R, Kakizuka A, Ohkubo H, Nakanishi S. 1989. Molecular characterization of a functional cDNA for rat substance P receptor. Journal of Biological Chemistry. 264(30), 17649–17652.","ieee":"Y. Yokota <i>et al.</i>, “Molecular characterization of a functional cDNA for rat substance P receptor,” <i>Journal of Biological Chemistry</i>, vol. 264, no. 30. American Society for Biochemistry and Molecular Biology, pp. 17649–17652, 1989.","short":"Y. Yokota, Y. Sasai, K. Tanaka, T. Fujiwara, K. Tsuchida, R. Shigemoto, A. Kakizuka, H. Ohkubo, S. Nakanishi, Journal of Biological Chemistry 264 (1989) 17649–17652.","mla":"Yokota, Yoshifumi, et al. “Molecular Characterization of a Functional CDNA for Rat Substance P Receptor.” <i>Journal of Biological Chemistry</i>, vol. 264, no. 30, American Society for Biochemistry and Molecular Biology, 1989, pp. 17649–52, doi:<a href=\"https://doi.org/10.1016/S0021-9258(19)84619-7\">10.1016/S0021-9258(19)84619-7</a>.","chicago":"Yokota, Yoshifumi, Yoshiki Sasai, Kohichi Tanaka, Tsutomu Fujiwara, Kunihiro Tsuchida, Ryuichi Shigemoto, Akira Kakizuka, Hiroaki Ohkubo, and Shigetada Nakanishi. “Molecular Characterization of a Functional CDNA for Rat Substance P Receptor.” <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology, 1989. <a href=\"https://doi.org/10.1016/S0021-9258(19)84619-7\">https://doi.org/10.1016/S0021-9258(19)84619-7</a>."},"doi":"10.1016/S0021-9258(19)84619-7","year":"1989","abstract":[{"lang":"eng","text":"This paper describes the amino acid sequence of the rat substance P receptor and its comparison with that of the rat substance K receptor on the basis of molecular cloning and sequence analysis. From a rat brain cDNA library constructed with an RNA expression vector, we identified a cDNA mixture containing a functional substance P receptor cDNA by examining electrophysiologically a receptor expression following injection of the mRNAs synthesized in vitro into Xenopus oocytes. A receptor cDNA clone was then isolated by cross-hybridization with the bovine substance K receptor DNA. The clone was confirmed by selective binding of substance P to the cloned receptor expressed in mammalian COS cells. The deduced amino acid sequence (407 amino acid residues) possesses seven putative membrane spanning domains and shows a sequence similarity to the members of G-protein-coupled receptors. The rat substance P and substance K receptor are very similar in both size and amino acid sequences, particularly in the putative transmembrane similarity is in marked contrast to the sequence divergence in the amino- and carboxyl-terminal regions and the third cytoplasmic loop. The observed sequence similarytity and divergence would thus contribute to the expression of similar but pharmacological regions and the first and second cytoplasmic loops. This distinguishable activities of the two tachykinin receptors."}],"acknowledgement":"This work was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, the Institute of Physical and Chemical Research, and the Science and Technology Agency of Japan. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. ","scopus_import":"1","intvolume":"       264","external_id":{"pmid":["2478537"]},"month":"10","quality_controlled":"1","status":"public","type":"journal_article","title":"Molecular characterization of a functional cDNA for rat substance P receptor","publisher":"American Society for Biochemistry and Molecular Biology","publication_status":"published","date_published":"1989-10-25T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2018-12-11T11:58:11Z","_id":"2525","publication":"Journal of Biological Chemistry","page":"17649 - 17652","day":"25"}]