---
_id: '2533'
abstract:
- lang: eng
  text: A cDNA clone for a new metabotropic glutamate receptor, mGluR5, was isolated
    through polymerase chain reaction-mediated DNA amplification by using primer sequences
    conserved among the metabotropic glutamate receptor (mGluR) family and by the
    subsequent screening of a rat brain cDNA library. The cloned receptor consists
    of 1171 amino acid residues and exhibits a structural architecture common to the
    mGluR family, possessing a large extracellular domain preceding the seven putative
    membrane-spanning segments. mGluR5 shows the highest sequence similarity to mGluR1
    among the mGluR members and is coupled to the stimulation of phosphatidylinositol
    hydrolysis/ Ca2+ signal transduction in Chinese hamster ovary cells transfected
    with the cloned cDNA. This receptor also resembles mGluR1 in its agonist selectivity
    and antagonist responses; the potency rank order of agonists for mGluR5 was determined
    to be quisqualate > L-glutamate ≥ ibotenate > trans-1-aminocyclopentane-1,3-dicarboxylate.
    Blot and in situ hybridization analyses indicated that mGluR5 mRNA is widely distributed
    in neuronal cells of the central nervous system and is expressed differently from
    mGluR1 mRNA in many brain regions. This investigation thus demonstrates that there
    is an additional mGluR subtype which closely resembles mGluR1 in its signal transduction
    and pharmacological properties and is expressed in specialized neuronal cells
    in the central nervous system.
acknowledgement: We are grateful to Seiji Ito for help of Ca2+ measurements and Akira
  Uesugi for photographic assistance.
article_processing_charge: No
article_type: original
author:
- first_name: Takaaki
  full_name: Abe, Takaaki
  last_name: Abe
- first_name: Hidemitsu
  full_name: Sugihara, Hidemitsu
  last_name: Sugihara
- first_name: Hiroyuki
  full_name: Nawa, Hiroyuki
  last_name: Nawa
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Noboru
  full_name: Mizuno, Noboru
  last_name: Mizuno
- first_name: Shigetada
  full_name: Nakanishi, Shigetada
  last_name: Nakanishi
citation:
  ama: Abe T, Sugihara H, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. Molecular characterization
    of a novel metabotropic glutamate receptor mGluR5 coupled to inositol phosphate/Ca2+
    signal transduction. <i>Journal of Biological Chemistry</i>. 1992;267(19):13361-13368.
    doi:<a href="https://doi.org/10.1016/S0021-9258(18)42219-3">10.1016/S0021-9258(18)42219-3</a>
  apa: Abe, T., Sugihara, H., Nawa, H., Shigemoto, R., Mizuno, N., &#38; Nakanishi,
    S. (1992). Molecular characterization of a novel metabotropic glutamate receptor
    mGluR5 coupled to inositol phosphate/Ca2+ signal transduction. <i>Journal of Biological
    Chemistry</i>. American Society for Biochemistry and Molecular Biology. <a href="https://doi.org/10.1016/S0021-9258(18)42219-3">https://doi.org/10.1016/S0021-9258(18)42219-3</a>
  chicago: Abe, Takaaki, Hidemitsu Sugihara, Hiroyuki Nawa, Ryuichi Shigemoto, Noboru
    Mizuno, and Shigetada Nakanishi. “Molecular Characterization of a Novel Metabotropic
    Glutamate Receptor MGluR5 Coupled to Inositol Phosphate/Ca2+ Signal Transduction.”
    <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and
    Molecular Biology, 1992. <a href="https://doi.org/10.1016/S0021-9258(18)42219-3">https://doi.org/10.1016/S0021-9258(18)42219-3</a>.
  ieee: T. Abe, H. Sugihara, H. Nawa, R. Shigemoto, N. Mizuno, and S. Nakanishi, “Molecular
    characterization of a novel metabotropic glutamate receptor mGluR5 coupled to
    inositol phosphate/Ca2+ signal transduction,” <i>Journal of Biological Chemistry</i>,
    vol. 267, no. 19. American Society for Biochemistry and Molecular Biology, pp.
    13361–13368, 1992.
  ista: Abe T, Sugihara H, Nawa H, Shigemoto R, Mizuno N, Nakanishi S. 1992. Molecular
    characterization of a novel metabotropic glutamate receptor mGluR5 coupled to
    inositol phosphate/Ca2+ signal transduction. Journal of Biological Chemistry.
    267(19), 13361–13368.
  mla: Abe, Takaaki, et al. “Molecular Characterization of a Novel Metabotropic Glutamate
    Receptor MGluR5 Coupled to Inositol Phosphate/Ca2+ Signal Transduction.” <i>Journal
    of Biological Chemistry</i>, vol. 267, no. 19, American Society for Biochemistry
    and Molecular Biology, 1992, pp. 13361–68, doi:<a href="https://doi.org/10.1016/S0021-9258(18)42219-3">10.1016/S0021-9258(18)42219-3</a>.
  short: T. Abe, H. Sugihara, H. Nawa, R. Shigemoto, N. Mizuno, S. Nakanishi, Journal
    of Biological Chemistry 267 (1992) 13361–13368.
date_created: 2018-12-11T11:58:14Z
date_published: 1992-07-05T00:00:00Z
date_updated: 2022-03-17T15:08:29Z
day: '05'
doi: 10.1016/S0021-9258(18)42219-3
extern: '1'
external_id:
  pmid:
  - '1320017'
fulldoi: https://doi.org/10.1016/S0021-9258(18)42219-3
intvolume: '       267'
issue: '19'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.sciencedirect.com/science/article/pii/S0021925818422193
month: '07'
oa: 1
oa_version: Published Version
page: 13361 - 13368
pmid: 1
publication: Journal of Biological Chemistry
publication_identifier:
  issn:
  - 0021-9258
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
publist_id: '4366'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular characterization of a novel metabotropic glutamate receptor mGluR5
  coupled to inositol phosphate/Ca2+ signal transduction
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 267
year: '1992'
...
---
_id: '2480'
abstract:
- lang: eng
  text: Functional cDNA clones for rat neuromedin K receptor were isolated from a
    rat brain cDNA library by cross-hybridization with the bovine substance K recepor
    cDNA. Injection of the mRNA synthesized in vitro from the cloned cDNA into Xenopus
    oocytes elicited electrophysiological responses to tachykinins, with the most
    potent sensitivity being to neuromedin K. Ligand-binding displacement in membranes
    of mammalian COS cells transfected with the cDNA indicated the rank order of affinity
    of the receptor to tachykinins; neuromedin K &gt; substance K &gt; substance P.
    The hybridization analysis showed that the neuromedin K receptor mRNA is expressed
    in both the brain and the peripheral tissues at different levels. The rat neuromedin
    K receptor consists of 452 amino acid residues and belongs to the family of G
    protein-coupled receptors, which are thought to have seven transmembrane domains.
    The sequence comparison of the rat neuromedin K, substance P, and substance K
    receptors revealed that these receptors are highly conserved in the seven transmembrane
    domains and the cytoplasmic sides of the receptors. They also show some structural
    characteristics, including the common presence of histidine residues in transmembrane
    segments V and VI and the difference in the numbers and distributions of serine
    and threonine residues as possible phosphorylation sites in the cytoplasmic regions.
    This paper thus presents the first comprehensive analysis of the molecular nature
    of the multiple peptide receptors that exhibit similar but pharmacologically distinguishable
    activities.
acknowledgement: This work was supported in part by research grants from the Ministry
  Education, Science and Culture of Japan; the Institute of Physical and Chemical
  Research; and the Science and Technology Agency of Japan. The costs of publication
  of this article were defrayed in part by the payment of page charges. This article
  must therefore be hereby marked “advertisement” in accordance with 18 USC. Section
  1734 solely to indicate this fact.
article_processing_charge: No
article_type: original
author:
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Yoshifumi
  full_name: Yokota, Yoshifumi
  last_name: Yokota
- first_name: Kunihiro
  full_name: Tsuchida, Kunihiro
  last_name: Tsuchida
- first_name: Shigetada
  full_name: Nakanishi, Shigetada
  last_name: Nakanishi
citation:
  ama: Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S. Cloning and expression of a
    rat neuromedin K receptor cDNA. <i>Journal of Biological Chemistry</i>. 1990;265(2):623-628.
    doi:<a href="https://doi.org/10.1016/s0021-9258(19)40095-1 ">10.1016/s0021-9258(19)40095-1
    </a>
  apa: Shigemoto, R., Yokota, Y., Tsuchida, K., &#38; Nakanishi, S. (1990). Cloning
    and expression of a rat neuromedin K receptor cDNA. <i>Journal of Biological Chemistry</i>.
    American Society for Biochemistry and Molecular Biology. <a href="https://doi.org/10.1016/s0021-9258(19)40095-1
    ">https://doi.org/10.1016/s0021-9258(19)40095-1 </a>
  chicago: Shigemoto, Ryuichi, Yoshifumi Yokota, Kunihiro Tsuchida, and Shigetada
    Nakanishi. “Cloning and Expression of a Rat Neuromedin K Receptor CDNA.” <i>Journal
    of Biological Chemistry</i>. American Society for Biochemistry and Molecular Biology,
    1990. <a href="https://doi.org/10.1016/s0021-9258(19)40095-1 ">https://doi.org/10.1016/s0021-9258(19)40095-1
    </a>.
  ieee: R. Shigemoto, Y. Yokota, K. Tsuchida, and S. Nakanishi, “Cloning and expression
    of a rat neuromedin K receptor cDNA,” <i>Journal of Biological Chemistry</i>,
    vol. 265, no. 2. American Society for Biochemistry and Molecular Biology, pp.
    623–628, 1990.
  ista: Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S. 1990. Cloning and expression
    of a rat neuromedin K receptor cDNA. Journal of Biological Chemistry. 265(2),
    623–628.
  mla: Shigemoto, Ryuichi, et al. “Cloning and Expression of a Rat Neuromedin K Receptor
    CDNA.” <i>Journal of Biological Chemistry</i>, vol. 265, no. 2, American Society
    for Biochemistry and Molecular Biology, 1990, pp. 623–28, doi:<a href="https://doi.org/10.1016/s0021-9258(19)40095-1
    ">10.1016/s0021-9258(19)40095-1 </a>.
  short: R. Shigemoto, Y. Yokota, K. Tsuchida, S. Nakanishi, Journal of Biological
    Chemistry 265 (1990) 623–628.
date_created: 2018-12-11T11:57:55Z
date_published: 1990-01-15T00:00:00Z
date_updated: 2022-02-24T11:07:05Z
day: '15'
doi: '10.1016/s0021-9258(19)40095-1 '
extern: '1'
external_id:
  pmid:
  - '2153106 '
fulldoi: 'https://doi.org/10.1016/s0021-9258(19)40095-1 '
intvolume: '       265'
issue: '2'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.sciencedirect.com/science/article/pii/S0021925819400951
month: '01'
oa: 1
oa_version: Published Version
page: 623 - 628
pmid: 1
publication: Journal of Biological Chemistry
publication_identifier:
  eissn:
  - 1083-351X
  issn:
  - 0021-9258
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
publist_id: '4421'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Cloning and expression of a rat neuromedin K receptor cDNA
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 265
year: '1990'
...
---
_id: '2525'
abstract:
- lang: eng
  text: This paper describes the amino acid sequence of the rat substance P receptor
    and its comparison with that of the rat substance K receptor on the basis of molecular
    cloning and sequence analysis. From a rat brain cDNA library constructed with
    an RNA expression vector, we identified a cDNA mixture containing a functional
    substance P receptor cDNA by examining electrophysiologically a receptor expression
    following injection of the mRNAs synthesized in vitro into Xenopus oocytes. A
    receptor cDNA clone was then isolated by cross-hybridization with the bovine substance
    K receptor DNA. The clone was confirmed by selective binding of substance P to
    the cloned receptor expressed in mammalian COS cells. The deduced amino acid sequence
    (407 amino acid residues) possesses seven putative membrane spanning domains and
    shows a sequence similarity to the members of G-protein-coupled receptors. The
    rat substance P and substance K receptor are very similar in both size and amino
    acid sequences, particularly in the putative transmembrane similarity is in marked
    contrast to the sequence divergence in the amino- and carboxyl-terminal regions
    and the third cytoplasmic loop. The observed sequence similarytity and divergence
    would thus contribute to the expression of similar but pharmacological regions
    and the first and second cytoplasmic loops. This distinguishable activities of
    the two tachykinin receptors.
acknowledgement: 'This work was supported in part by research grants from the Ministry
  of Education, Science and Culture of Japan, the Institute of Physical and Chemical
  Research, and the Science and Technology Agency of Japan. The costs of publication
  of this article were defrayed in part by the payment of page charges. This article
  must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section
  1734 solely to indicate this fact. '
article_processing_charge: No
article_type: original
author:
- first_name: Yoshifumi
  full_name: Yokota, Yoshifumi
  last_name: Yokota
- first_name: Yoshiki
  full_name: Sasai, Yoshiki
  last_name: Sasai
- first_name: Kohichi
  full_name: Tanaka, Kohichi
  last_name: Tanaka
- first_name: Tsutomu
  full_name: Fujiwara, Tsutomu
  last_name: Fujiwara
- first_name: Kunihiro
  full_name: Tsuchida, Kunihiro
  last_name: Tsuchida
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Akira
  full_name: Kakizuka, Akira
  last_name: Kakizuka
- first_name: Hiroaki
  full_name: Ohkubo, Hiroaki
  last_name: Ohkubo
- first_name: Shigetada
  full_name: Nakanishi, Shigetada
  last_name: Nakanishi
citation:
  ama: Yokota Y, Sasai Y, Tanaka K, et al. Molecular characterization of a functional
    cDNA for rat substance P receptor. <i>Journal of Biological Chemistry</i>. 1989;264(30):17649-17652.
    doi:<a href="https://doi.org/10.1016/S0021-9258(19)84619-7">10.1016/S0021-9258(19)84619-7</a>
  apa: Yokota, Y., Sasai, Y., Tanaka, K., Fujiwara, T., Tsuchida, K., Shigemoto, R.,
    … Nakanishi, S. (1989). Molecular characterization of a functional cDNA for rat
    substance P receptor. <i>Journal of Biological Chemistry</i>. American Society
    for Biochemistry and Molecular Biology. <a href="https://doi.org/10.1016/S0021-9258(19)84619-7">https://doi.org/10.1016/S0021-9258(19)84619-7</a>
  chicago: Yokota, Yoshifumi, Yoshiki Sasai, Kohichi Tanaka, Tsutomu Fujiwara, Kunihiro
    Tsuchida, Ryuichi Shigemoto, Akira Kakizuka, Hiroaki Ohkubo, and Shigetada Nakanishi.
    “Molecular Characterization of a Functional CDNA for Rat Substance P Receptor.”
    <i>Journal of Biological Chemistry</i>. American Society for Biochemistry and
    Molecular Biology, 1989. <a href="https://doi.org/10.1016/S0021-9258(19)84619-7">https://doi.org/10.1016/S0021-9258(19)84619-7</a>.
  ieee: Y. Yokota <i>et al.</i>, “Molecular characterization of a functional cDNA
    for rat substance P receptor,” <i>Journal of Biological Chemistry</i>, vol. 264,
    no. 30. American Society for Biochemistry and Molecular Biology, pp. 17649–17652,
    1989.
  ista: Yokota Y, Sasai Y, Tanaka K, Fujiwara T, Tsuchida K, Shigemoto R, Kakizuka
    A, Ohkubo H, Nakanishi S. 1989. Molecular characterization of a functional cDNA
    for rat substance P receptor. Journal of Biological Chemistry. 264(30), 17649–17652.
  mla: Yokota, Yoshifumi, et al. “Molecular Characterization of a Functional CDNA
    for Rat Substance P Receptor.” <i>Journal of Biological Chemistry</i>, vol. 264,
    no. 30, American Society for Biochemistry and Molecular Biology, 1989, pp. 17649–52,
    doi:<a href="https://doi.org/10.1016/S0021-9258(19)84619-7">10.1016/S0021-9258(19)84619-7</a>.
  short: Y. Yokota, Y. Sasai, K. Tanaka, T. Fujiwara, K. Tsuchida, R. Shigemoto, A.
    Kakizuka, H. Ohkubo, S. Nakanishi, Journal of Biological Chemistry 264 (1989)
    17649–17652.
date_created: 2018-12-11T11:58:11Z
date_published: 1989-10-25T00:00:00Z
date_updated: 2025-06-26T09:18:02Z
day: '25'
doi: 10.1016/S0021-9258(19)84619-7
extern: '1'
external_id:
  pmid:
  - '2478537'
fulldoi: https://doi.org/10.1016/S0021-9258(19)84619-7
intvolume: '       264'
issue: '30'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/S0021-9258(19)84619-7
month: '10'
oa: 1
oa_version: Published Version
page: 17649 - 17652
pmid: 1
publication: Journal of Biological Chemistry
publication_identifier:
  eissn:
  - 1083-351X
  issn:
  - 0021-9258
publication_status: published
publisher: American Society for Biochemistry and Molecular Biology
publist_id: '4374'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Molecular characterization of a functional cDNA for rat substance P receptor
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 264
year: '1989'
...