[{"year":"1993","quality_controlled":"1","volume":335,"language":[{"iso":"eng"}],"article_processing_charge":"No","page":"252 - 266","issue":"2","month":"09","doi":"10.1002/cne.903350209","abstract":[{"text":"Distribution of the mRNA for a metabotropic glutamate receptor, mGluR3, which is coupled to the inhibitory cAMP cascade, was examined in the central nervous system of the adult albino rat by in situ hybridization. The hybridization signals of mGluR3 were detected not only on neuronal cells but also on many glial cells throughout the brain and spinal cord. In the neuronal cells, prominent expression of mGluR3 mRNA was seen in the thalamic reticular nucleus. Moderately labeled neurons were seen in the anterior olfactory nucleus, cerebral neo- and mesocortical regions, lateral amygdaloid nucleus, ventral part of the basolateral amygdaloid nucleus, dorsal endopiriform nucleus, supraoptic nucleus, superficial layers of the superior colliculus, inferior colliculus, interpeduncular nucleus, superior olivary nuclei, and Golgi cells in the cerebellar cortex. Weakly labeled neurons were observed in the striatum, nucleus accumbens, ventral pallidum, globus pallidus, entopeduncular nucleus, lateral hypothalamic area, hypothalamic paraventricular nucleus, medial habenular nucleus, anterior pretectal nucleus, Barrington's nucleus, Nucleus O, paragenual nucleus, trigeminal sensory complex, cochlear nuclei, dorsal motor nucleus of the trigeminal nerve, dorsal cap of the inferior olive, spinal dorsal horn, and lamina X of the spinal cord. The stellate cells in the cerebellar cortex, and neurons in the deep cerebellar nuclei were also labeled weakly. The granule cell layer of the dentate gyrus, as a whole, appeared to be labeled intensely, but each of the granule cells was labeled only weakly. No significant labeling was detected in the mitral and tufted cells in the olfactory bulb, hippocampal pyramidal cells, Purkinje and granule cells in the cerebellar cortex, or somatic motoneurons. The distribution of mGluR3 mRNA in particular neurons and glial cells indicates specific roles of mGluR3 in the glutamatergic system of the central nervous system.","lang":"eng"}],"citation":{"apa":"Ohishi, H., Shigemoto, R., Nakanishi, S., &#38; Mizuno, N. (1993).  Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization study. <i>Journal of Comparative Neurology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/cne.903350209\">https://doi.org/10.1002/cne.903350209</a>","mla":"Ohishi, Hitoshi, et al. “ Distribution of the MRNA for a Metabotropic Glutamate Receptor (MGluR3) in the Rat Brain: An in Situ Hybridization Study.” <i>Journal of Comparative Neurology</i>, vol. 335, no. 2, Wiley-Blackwell, 1993, pp. 252–66, doi:<a href=\"https://doi.org/10.1002/cne.903350209\">10.1002/cne.903350209</a>.","ama":"Ohishi H, Shigemoto R, Nakanishi S, Mizuno N.  Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization study. <i>Journal of Comparative Neurology</i>. 1993;335(2):252-266. doi:<a href=\"https://doi.org/10.1002/cne.903350209\">10.1002/cne.903350209</a>","short":"H. Ohishi, R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative Neurology 335 (1993) 252–266.","ieee":"H. Ohishi, R. Shigemoto, S. Nakanishi, and N. Mizuno, “ Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization study,” <i>Journal of Comparative Neurology</i>, vol. 335, no. 2. Wiley-Blackwell, pp. 252–266, 1993.","ista":"Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. 1993.  Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization study. Journal of Comparative Neurology. 335(2), 252–266.","chicago":"Ohishi, Hitoshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno. “ Distribution of the MRNA for a Metabotropic Glutamate Receptor (MGluR3) in the Rat Brain: An in Situ Hybridization Study.” <i>Journal of Comparative Neurology</i>. Wiley-Blackwell, 1993. <a href=\"https://doi.org/10.1002/cne.903350209\">https://doi.org/10.1002/cne.903350209</a>."},"publisher":"Wiley-Blackwell","type":"journal_article","publication_status":"published","intvolume":"       335","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_updated":"2022-06-01T11:58:11Z","status":"public","main_file_link":[{"url":"https://onlinelibrary.wiley.com/doi/10.1002/cne.903350209"}],"title":" Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization study","author":[{"full_name":"Ohishi, Hitoshi","last_name":"Ohishi","first_name":"Hitoshi"},{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Shigetada","last_name":"Nakanishi","full_name":"Nakanishi, Shigetada"},{"full_name":"Mizuno, Noboru","last_name":"Mizuno","first_name":"Noboru"}],"day":"08","article_type":"original","acknowledgement":"We are grateful for the photographic help of Mr. Akira Uesugi and the support of Drs. Satoru Fukuchi, Toshio Fukuda, Ritsu Hayashi, Mizuho Katsurada, Yutaka Kitani, Keiko Kumagai, Toshihiko  Kuroda,  Hiroshi Matsubara, Hiroshi Matsushima, Chisato Minakuchi, Masatoshi Nishio, Gonpei Niwa,  Hajime Oda, Masahiko Ohbayashi, Sei-ichi Ohbayashi, Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watan- abe, Kazuo Yoshino, and Toshiaki Yoshino. This work was supported in  part by grants-in-aid from the Ministry of Education, Science, and Culture of Japan.","publication_identifier":{"issn":["0021-9967"]},"date_published":"1993-09-08T00:00:00Z","external_id":{"pmid":["8227517"]},"pmid":1,"_id":"2487","oa_version":"None","date_created":"2018-12-11T11:57:57Z","publist_id":"4414","publication":"Journal of Comparative Neurology","extern":"1"},{"extern":"1","publication":"Journal of Comparative Neurology","publist_id":"4415","oa_version":"Published Version","date_created":"2018-12-11T11:57:57Z","pmid":1,"_id":"2486","external_id":{"pmid":["1430307"]},"date_published":"1992-08-01T00:00:00Z","publication_identifier":{"issn":["0021-9967"]},"article_type":"original","acknowledgement":"We are grateful to Mr.  Akira Uesugi for photographic help. This work was supported in part by research grants from Senri Life Science Foundation and the Ministry of Education, Science and Culture of Japan.","day":"01","title":"Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization study in adult and developing rat","main_file_link":[{"url":"https://onlinelibrary.wiley.com/doi/10.1002/cne.903220110"}],"author":[{"orcid":"0000-0001-8761-9444","full_name":"Shigemoto, Ryuichi","last_name":"Shigemoto","first_name":"Ryuichi","id":"499F3ABC-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Nakanishi, Shigetada","last_name":"Nakanishi","first_name":"Shigetada"},{"last_name":"Mizuno","first_name":"Noboru","full_name":"Mizuno, Noboru"}],"status":"public","date_updated":"2022-03-21T09:41:37Z","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","intvolume":"       322","publication_status":"published","publisher":"Wiley-Blackwell","type":"journal_article","citation":{"short":"R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative Neurology 322 (1992) 121–135.","ama":"Shigemoto R, Nakanishi S, Mizuno N. Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization study in adult and developing rat. <i>Journal of Comparative Neurology</i>. 1992;322(1):121-135. doi:<a href=\"https://doi.org/10.1002/cne.903220110\">10.1002/cne.903220110</a>","chicago":"Shigemoto, Ryuichi, Shigetada Nakanishi, and Noboru Mizuno. “Distribution of the MRNA for a Metabotropic Glutamate Receptor (MGluR1) in the Central Nervous System: An in Situ Hybridization Study in Adult and Developing Rat.” <i>Journal of Comparative Neurology</i>. Wiley-Blackwell, 1992. <a href=\"https://doi.org/10.1002/cne.903220110\">https://doi.org/10.1002/cne.903220110</a>.","ieee":"R. Shigemoto, S. Nakanishi, and N. Mizuno, “Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization study in adult and developing rat,” <i>Journal of Comparative Neurology</i>, vol. 322, no. 1. Wiley-Blackwell, pp. 121–135, 1992.","ista":"Shigemoto R, Nakanishi S, Mizuno N. 1992. Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization study in adult and developing rat. Journal of Comparative Neurology. 322(1), 121–135.","mla":"Shigemoto, Ryuichi, et al. “Distribution of the MRNA for a Metabotropic Glutamate Receptor (MGluR1) in the Central Nervous System: An in Situ Hybridization Study in Adult and Developing Rat.” <i>Journal of Comparative Neurology</i>, vol. 322, no. 1, Wiley-Blackwell, 1992, pp. 121–35, doi:<a href=\"https://doi.org/10.1002/cne.903220110\">10.1002/cne.903220110</a>.","apa":"Shigemoto, R., Nakanishi, S., &#38; Mizuno, N. (1992). Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization study in adult and developing rat. <i>Journal of Comparative Neurology</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1002/cne.903220110\">https://doi.org/10.1002/cne.903220110</a>"},"abstract":[{"text":"Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1), which is linked to phosphoinositide (PI) hydrolysis, was investigated in adult and developing rat central nervous system (CNS) by in situ hybridization. Transcripts of mGluR1 were specifically localized to neurons and widely distributed throughout the adult rat brain. Most intensely labeled neurons were Purkinje cells of the cerebellum, mitral and tufted cells of the olfactory bulb, and neurons in the hippocampus, lateral septum, thalamus, globus pallidus, entopeduncular nucleus, ventral pallidum, magnocellular preoptic nucleus, substantia nigra, and dorsal cochlear nucleus. Moderately labeled neurons were seen in high density in the dentate gyrus, striatum, islands of Calleja, superficial layers of the retrosplenial, cingulate and entorhinal cortices, mammillary nuclei, red nucleus, and superior colliculus. In the developing rat brain, the level of mGluR1 expression gradually increased during early postnatal days in accordance with the maturation of neuronal elements. These results show prominent expression of mGluR1 in the major targets of putative glutamatergic pathways and unique distribution pattern of mGluR1 distinct from those reported for ionotropic subtypes of glutamate receptors, suggesting specific roles of mGluR1 in the glutamatergic system.","lang":"eng"}],"scopus_import":"1","doi":"10.1002/cne.903220110","month":"08","page":"121 - 135","issue":"1","article_processing_charge":"No","language":[{"iso":"eng"}],"volume":322,"quality_controlled":"1","year":"1992"}]