---
_id: '2487'
abstract:
- lang: eng
text: Distribution of the mRNA for a metabotropic glutamate receptor, mGluR3, which
is coupled to the inhibitory cAMP cascade, was examined in the central nervous
system of the adult albino rat by in situ hybridization. The hybridization signals
of mGluR3 were detected not only on neuronal cells but also on many glial cells
throughout the brain and spinal cord. In the neuronal cells, prominent expression
of mGluR3 mRNA was seen in the thalamic reticular nucleus. Moderately labeled
neurons were seen in the anterior olfactory nucleus, cerebral neo- and mesocortical
regions, lateral amygdaloid nucleus, ventral part of the basolateral amygdaloid
nucleus, dorsal endopiriform nucleus, supraoptic nucleus, superficial layers of
the superior colliculus, inferior colliculus, interpeduncular nucleus, superior
olivary nuclei, and Golgi cells in the cerebellar cortex. Weakly labeled neurons
were observed in the striatum, nucleus accumbens, ventral pallidum, globus pallidus,
entopeduncular nucleus, lateral hypothalamic area, hypothalamic paraventricular
nucleus, medial habenular nucleus, anterior pretectal nucleus, Barrington's nucleus,
Nucleus O, paragenual nucleus, trigeminal sensory complex, cochlear nuclei, dorsal
motor nucleus of the trigeminal nerve, dorsal cap of the inferior olive, spinal
dorsal horn, and lamina X of the spinal cord. The stellate cells in the cerebellar
cortex, and neurons in the deep cerebellar nuclei were also labeled weakly. The
granule cell layer of the dentate gyrus, as a whole, appeared to be labeled intensely,
but each of the granule cells was labeled only weakly. No significant labeling
was detected in the mitral and tufted cells in the olfactory bulb, hippocampal
pyramidal cells, Purkinje and granule cells in the cerebellar cortex, or somatic
motoneurons. The distribution of mGluR3 mRNA in particular neurons and glial cells
indicates specific roles of mGluR3 in the glutamatergic system of the central
nervous system.
acknowledgement: We are grateful for the photographic help of Mr. Akira Uesugi and
the support of Drs. Satoru Fukuchi, Toshio Fukuda, Ritsu Hayashi, Mizuho Katsurada,
Yutaka Kitani, Keiko Kumagai, Toshihiko Kuroda, Hiroshi Matsubara, Hiroshi Matsushima,
Chisato Minakuchi, Masatoshi Nishio, Gonpei Niwa, Hajime Oda, Masahiko Ohbayashi,
Sei-ichi Ohbayashi, Hiroyasu Ohtsuka, Shigeo Tamaki, Eizo Watan- abe, Kazuo Yoshino,
and Toshiaki Yoshino. This work was supported in part by grants-in-aid from the
Ministry of Education, Science, and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Hitoshi
full_name: Ohishi, Hitoshi
last_name: Ohishi
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. Distribution of the mRNA for
a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ hybridization
study. Journal of Comparative Neurology. 1993;335(2):252-266. doi:10.1002/cne.903350209'
apa: 'Ohishi, H., Shigemoto, R., Nakanishi, S., & Mizuno, N. (1993). Distribution
of the mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An
in situ hybridization study. Journal of Comparative Neurology. Wiley-Blackwell.
https://doi.org/10.1002/cne.903350209'
chicago: 'Ohishi, Hitoshi, Ryuichi Shigemoto, Shigetada Nakanishi, and Noboru Mizuno.
“ Distribution of the MRNA for a Metabotropic Glutamate Receptor (MGluR3) in the
Rat Brain: An in Situ Hybridization Study.” Journal of Comparative Neurology.
Wiley-Blackwell, 1993. https://doi.org/10.1002/cne.903350209.'
ieee: 'H. Ohishi, R. Shigemoto, S. Nakanishi, and N. Mizuno, “ Distribution of the
mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ
hybridization study,” Journal of Comparative Neurology, vol. 335, no. 2.
Wiley-Blackwell, pp. 252–266, 1993.'
ista: 'Ohishi H, Shigemoto R, Nakanishi S, Mizuno N. 1993. Distribution of the
mRNA for a metabotropic glutamate receptor (mGluR3) in the rat brain: An in situ
hybridization study. Journal of Comparative Neurology. 335(2), 252–266.'
mla: 'Ohishi, Hitoshi, et al. “ Distribution of the MRNA for a Metabotropic Glutamate
Receptor (MGluR3) in the Rat Brain: An in Situ Hybridization Study.” Journal
of Comparative Neurology, vol. 335, no. 2, Wiley-Blackwell, 1993, pp. 252–66,
doi:10.1002/cne.903350209.'
short: H. Ohishi, R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative
Neurology 335 (1993) 252–266.
date_created: 2018-12-11T11:57:57Z
date_published: 1993-09-08T00:00:00Z
date_updated: 2022-06-01T11:58:11Z
day: '08'
doi: 10.1002/cne.903350209
extern: '1'
external_id:
pmid:
- '8227517'
intvolume: ' 335'
issue: '2'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1002/cne.903350209
month: '09'
oa_version: None
page: 252 - 266
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4414'
quality_controlled: '1'
status: public
title: ' Distribution of the mRNA for a metabotropic glutamate receptor (mGluR3) in
the rat brain: An in situ hybridization study'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 335
year: '1993'
...
---
_id: '2486'
abstract:
- lang: eng
text: Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1), which
is linked to phosphoinositide (PI) hydrolysis, was investigated in adult and developing
rat central nervous system (CNS) by in situ hybridization. Transcripts of mGluR1
were specifically localized to neurons and widely distributed throughout the adult
rat brain. Most intensely labeled neurons were Purkinje cells of the cerebellum,
mitral and tufted cells of the olfactory bulb, and neurons in the hippocampus,
lateral septum, thalamus, globus pallidus, entopeduncular nucleus, ventral pallidum,
magnocellular preoptic nucleus, substantia nigra, and dorsal cochlear nucleus.
Moderately labeled neurons were seen in high density in the dentate gyrus, striatum,
islands of Calleja, superficial layers of the retrosplenial, cingulate and entorhinal
cortices, mammillary nuclei, red nucleus, and superior colliculus. In the developing
rat brain, the level of mGluR1 expression gradually increased during early postnatal
days in accordance with the maturation of neuronal elements. These results show
prominent expression of mGluR1 in the major targets of putative glutamatergic
pathways and unique distribution pattern of mGluR1 distinct from those reported
for ionotropic subtypes of glutamate receptors, suggesting specific roles of mGluR1
in the glutamatergic system.
acknowledgement: We are grateful to Mr. Akira Uesugi for photographic help. This
work was supported in part by research grants from Senri Life Science Foundation
and the Ministry of Education, Science and Culture of Japan.
article_processing_charge: No
article_type: original
author:
- first_name: Ryuichi
full_name: Shigemoto, Ryuichi
id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
last_name: Shigemoto
orcid: 0000-0001-8761-9444
- first_name: Shigetada
full_name: Nakanishi, Shigetada
last_name: Nakanishi
- first_name: Noboru
full_name: Mizuno, Noboru
last_name: Mizuno
citation:
ama: 'Shigemoto R, Nakanishi S, Mizuno N. Distribution of the mRNA for a metabotropic
glutamate receptor (mGluR1) in the central nervous system: An in situ hybridization
study in adult and developing rat. Journal of Comparative Neurology. 1992;322(1):121-135.
doi:10.1002/cne.903220110'
apa: 'Shigemoto, R., Nakanishi, S., & Mizuno, N. (1992). Distribution of the
mRNA for a metabotropic glutamate receptor (mGluR1) in the central nervous system:
An in situ hybridization study in adult and developing rat. Journal of Comparative
Neurology. Wiley-Blackwell. https://doi.org/10.1002/cne.903220110'
chicago: 'Shigemoto, Ryuichi, Shigetada Nakanishi, and Noboru Mizuno. “Distribution
of the MRNA for a Metabotropic Glutamate Receptor (MGluR1) in the Central Nervous
System: An in Situ Hybridization Study in Adult and Developing Rat.” Journal
of Comparative Neurology. Wiley-Blackwell, 1992. https://doi.org/10.1002/cne.903220110.'
ieee: 'R. Shigemoto, S. Nakanishi, and N. Mizuno, “Distribution of the mRNA for
a metabotropic glutamate receptor (mGluR1) in the central nervous system: An in
situ hybridization study in adult and developing rat,” Journal of Comparative
Neurology, vol. 322, no. 1. Wiley-Blackwell, pp. 121–135, 1992.'
ista: 'Shigemoto R, Nakanishi S, Mizuno N. 1992. Distribution of the mRNA for a
metabotropic glutamate receptor (mGluR1) in the central nervous system: An in
situ hybridization study in adult and developing rat. Journal of Comparative Neurology.
322(1), 121–135.'
mla: 'Shigemoto, Ryuichi, et al. “Distribution of the MRNA for a Metabotropic Glutamate
Receptor (MGluR1) in the Central Nervous System: An in Situ Hybridization Study
in Adult and Developing Rat.” Journal of Comparative Neurology, vol. 322,
no. 1, Wiley-Blackwell, 1992, pp. 121–35, doi:10.1002/cne.903220110.'
short: R. Shigemoto, S. Nakanishi, N. Mizuno, Journal of Comparative Neurology 322
(1992) 121–135.
date_created: 2018-12-11T11:57:57Z
date_published: 1992-08-01T00:00:00Z
date_updated: 2022-03-21T09:41:37Z
day: '01'
doi: 10.1002/cne.903220110
extern: '1'
external_id:
pmid:
- '1430307'
intvolume: ' 322'
issue: '1'
language:
- iso: eng
main_file_link:
- url: https://onlinelibrary.wiley.com/doi/10.1002/cne.903220110
month: '08'
oa_version: Published Version
page: 121 - 135
pmid: 1
publication: Journal of Comparative Neurology
publication_identifier:
issn:
- 0021-9967
publication_status: published
publisher: Wiley-Blackwell
publist_id: '4415'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Distribution of the mRNA for a metabotropic glutamate receptor (mGluR1) in
the central nervous system: An in situ hybridization study in adult and developing
rat'
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 322
year: '1992'
...