[{"author":[{"last_name":"Gawish","first_name":"Riem","full_name":"Gawish, Riem"},{"full_name":"Varada, Rajagopal","last_name":"Varada","first_name":"Rajagopal"},{"full_name":"Deckert, Florian","last_name":"Deckert","first_name":"Florian"},{"full_name":"Hladik, Anastasiya","first_name":"Anastasiya","last_name":"Hladik"},{"first_name":"Linda","last_name":"Steinbichl","full_name":"Steinbichl, Linda"},{"full_name":"Cimatti, Laura","first_name":"Laura","last_name":"Cimatti"},{"first_name":"Katarina","last_name":"Milanovic","full_name":"Milanovic, Katarina"},{"full_name":"Jain, Mamta","last_name":"Jain","first_name":"Mamta"},{"first_name":"Natalya","last_name":"Torgasheva","full_name":"Torgasheva, Natalya"},{"last_name":"Tanzer","first_name":"Andrea","full_name":"Tanzer, Andrea"},{"last_name":"De Paepe","first_name":"Kim","full_name":"De Paepe, Kim"},{"last_name":"Van De Wiele","first_name":"Tom","full_name":"Van De Wiele, Tom"},{"last_name":"Hausmann","first_name":"Bela","full_name":"Hausmann, Bela"},{"last_name":"Lang","first_name":"Michaela","full_name":"Lang, Michaela"},{"last_name":"Pechhacker","first_name":"Martin","full_name":"Pechhacker, Martin"},{"full_name":"Ibrahim, Nahla","last_name":"Ibrahim","first_name":"Nahla"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"De Vries, Ingrid","last_name":"De Vries","first_name":"Ingrid"},{"last_name":"Brostjan","first_name":"Christine","full_name":"Brostjan, Christine"},{"last_name":"Sixt","orcid":"0000-0002-6620-9179","first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Gasche, Christoph","first_name":"Christoph","last_name":"Gasche"},{"last_name":"Boon","first_name":"Louis","full_name":"Boon, Louis"},{"first_name":"David","last_name":"Berry","full_name":"Berry, David"},{"full_name":"Jantsch, Michael F.","first_name":"Michael F.","last_name":"Jantsch"},{"first_name":"Fatima C.","last_name":"Pereira","full_name":"Pereira, Fatima C."},{"first_name":"Cornelia","last_name":"Vesely","full_name":"Vesely, Cornelia"}],"status":"public","issue":"9","publication":"Journal of Experimental Medicine","month":"09","ddc":["570"],"article_number":"e20240109","volume":222,"acknowledgement":"Sequencing was performed by the Vienna BioCenter Core Facilities (Medical University of Vienna Core Facility) and the Biomedical Sequencing Facility at CeMM, Vienna. Cell sorting and flow cytometry were performed at the Core Facility Flow Cytometry and Imaging (Medical University of Vienna). We thank Jasmin Schwarz, Gudrun Kohl, Petra Pjevac, and Joana Seneca Silva from the Joint Microbiome Facility of the Medical University of Vienna and the University of Vienna for assisting with amplicon and metagenomic sequencing, as well as repositing of sequencing data. We thank Sophia Derdak and Michael Schuster for initial data analysis, Robert Vilvoi and Stephan Hemm for animal handling, Marcel Kertesz for mouse genotyping, and Salwan Roumaia for next generation sequencing sample preparation. Treatment schemes and graphical abstracts were created with https://BioRender.com.\r\n\r\nThis work was supported by the Austrian Science Fund, grant number ZK 57-B28 to C. Vesely, R. Gawish, and F.C. Pereira; grant number V 1025-B to R. Gawish; grant number DOC32-B28 to R. Varada and M.F. Jantsch; and F8007 and P32678 to M.F. Jantsch. Open Access funding provided by Medical University of Vienna.","file":[{"checksum":"708d61fb8cf1d83ee1e33ddcfde0857e","relation":"main_file","access_level":"open_access","date_created":"2025-12-30T09:00:04Z","creator":"dernst","date_updated":"2025-12-30T09:00:04Z","content_type":"application/pdf","file_size":9349311,"file_id":"20899","success":1,"file_name":"2025_JEM_Gawish.pdf"}],"date_published":"2025-09-01T00:00:00Z","tmp":{"short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"citation":{"ista":"Gawish R, Varada R, Deckert F, Hladik A, Steinbichl L, Cimatti L, Milanovic K, Jain M, Torgasheva N, Tanzer A, De Paepe K, Van De Wiele T, Hausmann B, Lang M, Pechhacker M, Ibrahim N, de Vries I, Brostjan C, Sixt MK, Gasche C, Boon L, Berry D, Jantsch MF, Pereira FC, Vesely C. 2025. Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. Journal of Experimental Medicine. 222(9), e20240109.","apa":"Gawish, R., Varada, R., Deckert, F., Hladik, A., Steinbichl, L., Cimatti, L., … Vesely, C. (2025). Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. <i>Journal of Experimental Medicine</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1084/jem.20240109\">https://doi.org/10.1084/jem.20240109</a>","mla":"Gawish, Riem, et al. “Filamin A Editing in Myeloid Cells Reduces Intestinal Inflammation and Protects from Colitis.” <i>Journal of Experimental Medicine</i>, vol. 222, no. 9, e20240109, Rockefeller University Press, 2025, doi:<a href=\"https://doi.org/10.1084/jem.20240109\">10.1084/jem.20240109</a>.","short":"R. Gawish, R. Varada, F. Deckert, A. Hladik, L. Steinbichl, L. Cimatti, K. Milanovic, M. Jain, N. Torgasheva, A. Tanzer, K. De Paepe, T. Van De Wiele, B. Hausmann, M. Lang, M. Pechhacker, N. Ibrahim, I. de Vries, C. Brostjan, M.K. Sixt, C. Gasche, L. Boon, D. Berry, M.F. Jantsch, F.C. Pereira, C. Vesely, Journal of Experimental Medicine 222 (2025).","ieee":"R. Gawish <i>et al.</i>, “Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis,” <i>Journal of Experimental Medicine</i>, vol. 222, no. 9. Rockefeller University Press, 2025.","chicago":"Gawish, Riem, Rajagopal Varada, Florian Deckert, Anastasiya Hladik, Linda Steinbichl, Laura Cimatti, Katarina Milanovic, et al. “Filamin A Editing in Myeloid Cells Reduces Intestinal Inflammation and Protects from Colitis.” <i>Journal of Experimental Medicine</i>. Rockefeller University Press, 2025. <a href=\"https://doi.org/10.1084/jem.20240109\">https://doi.org/10.1084/jem.20240109</a>.","ama":"Gawish R, Varada R, Deckert F, et al. Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis. <i>Journal of Experimental Medicine</i>. 2025;222(9). doi:<a href=\"https://doi.org/10.1084/jem.20240109\">10.1084/jem.20240109</a>"},"abstract":[{"lang":"eng","text":"Patho-mechanistic origins of ulcerative colitis are still poorly understood. The actin cross-linker filamin A (FLNA) impacts cellular responses through interaction with cytosolic proteins. Posttranscriptional A-to-I editing generates two forms of FLNA: genome-encoded FLNAQ and FLNAR. FLNA is edited in colon fibroblasts, smooth muscle cells, and endothelial cells. We found that the FLNA editing status determines colitis severity. Editing was highest in healthy colons and reduced during murine and human colitis. Mice that exclusively express FLNAR were highly resistant to DSS-induced colitis, whereas fully FLNAQ animals developed severe inflammation. While the genetic induction of FLNA editing influenced transcriptional states of structural cells and microbiome composition, we found that FLNAR exerts protection specifically via myeloid cells, which are physiologically unedited. Introducing fixed FLNAR did not hamper cell migration but reduced macrophage inflammation and rendered neutrophils less prone to NETosis. Thus, loss of FLNA editing correlates with colitis severity, and targeted editing of myeloid cells serves as a novel therapeutic approach in intestinal inflammation."}],"has_accepted_license":"1","intvolume":"       222","date_updated":"2025-12-30T09:00:42Z","department":[{"_id":"MiSi"}],"publisher":"Rockefeller University Press","publication_status":"published","year":"2025","OA_type":"hybrid","oa":1,"day":"01","pmid":1,"external_id":{"pmid":["40471139"],"isi":["001502896900001"]},"type":"journal_article","title":"Filamin A editing in myeloid cells reduces intestinal inflammation and protects from colitis","article_processing_charge":"Yes (via OA deal)","license":"https://creativecommons.org/licenses/by/4.0/","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","publication_identifier":{"issn":["0022-1007"],"eissn":["1540-9538"]},"file_date_updated":"2025-12-30T09:00:04Z","OA_place":"publisher","language":[{"iso":"eng"}],"article_type":"original","scopus_import":"1","oa_version":"Published Version","_id":"19928","doi":"10.1084/jem.20240109","quality_controlled":"1","isi":1,"date_created":"2025-06-29T22:01:15Z"},{"issue":"7","publication":"The Journal of Experimental Medicine","author":[{"first_name":"Federica","last_name":"Moalli","full_name":"Moalli, Federica"},{"full_name":"Ficht, Xenia","first_name":"Xenia","last_name":"Ficht"},{"first_name":"Philipp","last_name":"Germann","full_name":"Germann, Philipp"},{"full_name":"Vladymyrov, Mykhailo","last_name":"Vladymyrov","first_name":"Mykhailo"},{"last_name":"Stolp","first_name":"Bettina","full_name":"Stolp, Bettina"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","full_name":"de Vries, Ingrid","first_name":"Ingrid","last_name":"de Vries"},{"full_name":"Lyck, Ruth","last_name":"Lyck","first_name":"Ruth"},{"last_name":"Balmer","first_name":"Jasmin","full_name":"Balmer, Jasmin"},{"first_name":"Amleto","last_name":"Fiocchi","full_name":"Fiocchi, Amleto"},{"full_name":"Kreutzfeldt, Mario","last_name":"Kreutzfeldt","first_name":"Mario"},{"last_name":"Merkler","first_name":"Doron","full_name":"Merkler, Doron"},{"last_name":"Iannacone","first_name":"Matteo","full_name":"Iannacone, Matteo"},{"first_name":"Akitaka","last_name":"Ariga","full_name":"Ariga, Akitaka"},{"full_name":"Stoffel, Michael H.","first_name":"Michael H.","last_name":"Stoffel"},{"full_name":"Sharpe, James","first_name":"James","last_name":"Sharpe"},{"first_name":"Martin","last_name":"Bähler","full_name":"Bähler, Martin"},{"first_name":"Michael K","last_name":"Sixt","orcid":"0000-0002-6620-9179","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","full_name":"Sixt, Michael K"},{"last_name":"Diz-Muñoz","first_name":"Alba","full_name":"Diz-Muñoz, Alba"},{"full_name":"Stein, Jens V.","first_name":"Jens V.","last_name":"Stein"}],"status":"public","file":[{"file_size":3841660,"content_type":"application/pdf","file_name":"2018_rupress_Moalli.pdf","file_id":"6498","relation":"main_file","checksum":"86ae5331f9bfced9a6358a790a04bef4","date_updated":"2020-07-14T12:47:32Z","creator":"kschuh","date_created":"2019-05-28T12:40:05Z","access_level":"open_access"}],"page":"1869–1890","month":"06","ddc":["570"],"volume":2015,"citation":{"ista":"Moalli F, Ficht X, Germann P, Vladymyrov M, Stolp B, de Vries I, Lyck R, Balmer J, Fiocchi A, Kreutzfeldt M, Merkler D, Iannacone M, Ariga A, Stoffel MH, Sharpe J, Bähler M, Sixt MK, Diz-Muñoz A, Stein JV. 2018. The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells. The Journal of Experimental Medicine. 2015(7), 1869–1890.","ama":"Moalli F, Ficht X, Germann P, et al. The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells. <i>The Journal of Experimental Medicine</i>. 2018;2015(7):1869–1890. doi:<a href=\"https://doi.org/10.1084/jem.20170896\">10.1084/jem.20170896</a>","chicago":"Moalli, Federica, Xenia Ficht, Philipp Germann, Mykhailo Vladymyrov, Bettina Stolp, Ingrid de Vries, Ruth Lyck, et al. “The Rho Regulator Myosin IXb Enables Nonlymphoid Tissue Seeding of Protective CD8+T Cells.” <i>The Journal of Experimental Medicine</i>. Rockefeller University Press, 2018. <a href=\"https://doi.org/10.1084/jem.20170896\">https://doi.org/10.1084/jem.20170896</a>.","mla":"Moalli, Federica, et al. “The Rho Regulator Myosin IXb Enables Nonlymphoid Tissue Seeding of Protective CD8+T Cells.” <i>The Journal of Experimental Medicine</i>, vol. 2015, no. 7, Rockefeller University Press, 2018, pp. 1869–1890, doi:<a href=\"https://doi.org/10.1084/jem.20170896\">10.1084/jem.20170896</a>.","ieee":"F. Moalli <i>et al.</i>, “The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells,” <i>The Journal of Experimental Medicine</i>, vol. 2015, no. 7. Rockefeller University Press, pp. 1869–1890, 2018.","short":"F. Moalli, X. Ficht, P. Germann, M. Vladymyrov, B. Stolp, I. de Vries, R. Lyck, J. Balmer, A. Fiocchi, M. Kreutzfeldt, D. Merkler, M. Iannacone, A. Ariga, M.H. Stoffel, J. Sharpe, M. Bähler, M.K. Sixt, A. Diz-Muñoz, J.V. Stein, The Journal of Experimental Medicine 2015 (2018) 1869–1890.","apa":"Moalli, F., Ficht, X., Germann, P., Vladymyrov, M., Stolp, B., de Vries, I., … Stein, J. V. (2018). The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells. <i>The Journal of Experimental Medicine</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1084/jem.20170896\">https://doi.org/10.1084/jem.20170896</a>"},"abstract":[{"lang":"eng","text":"T cells are actively scanning pMHC-presenting cells in lymphoid organs and nonlymphoid tissues (NLTs) with divergent topologies and confinement. How the T cell actomyosin cytoskeleton facilitates this task in distinct environments is incompletely understood. Here, we show that lack of Myosin IXb (Myo9b), a negative regulator of the small GTPase Rho, led to increased Rho-GTP levels and cell surface stiffness in primary T cells. Nonetheless, intravital imaging revealed robust motility of Myo9b−/− CD8+ T cells in lymphoid tissue and similar expansion and differentiation during immune responses. In contrast, accumulation of Myo9b−/− CD8+ T cells in NLTs was strongly impaired. Specifically, Myo9b was required for T cell crossing of basement membranes, such as those which are present between dermis and epidermis. As consequence, Myo9b−/− CD8+ T cells showed impaired control of skin infections. In sum, we show that Myo9b is critical for the CD8+ T cell adaptation from lymphoid to NLT surveillance and the establishment of protective tissue–resident T cell populations."}],"has_accepted_license":"1","date_published":"2018-06-06T00:00:00Z","tmp":{"image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"date_updated":"2023-09-19T14:52:08Z","intvolume":"      2015","oa":1,"day":"06","external_id":{"isi":["000440822900011"]},"type":"journal_article","department":[{"_id":"MiSi"}],"publisher":"Rockefeller University Press","year":"2018","publication_status":"published","license":"https://creativecommons.org/licenses/by-nc-sa/4.0/","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","publication_identifier":{"eissn":["1540-9538"],"issn":["0022-1007"]},"title":"The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells","article_processing_charge":"No","oa_version":"Published Version","scopus_import":"1","_id":"6497","doi":"10.1084/jem.20170896","file_date_updated":"2020-07-14T12:47:32Z","language":[{"iso":"eng"}],"isi":1,"date_created":"2019-05-28T12:36:47Z","quality_controlled":"1"},{"issue":"12","publication":"Journal of Experimental Medicine","status":"public","author":[{"first_name":"Anne","orcid":"0000-0003-0666-8928","last_name":"Reversat","full_name":"Reversat, Anne","id":"35B76592-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0000-0002-6620-9179","last_name":"Sixt","first_name":"Michael K","full_name":"Sixt, Michael K","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87"}],"page":"2959-2961","file":[{"file_id":"5931","file_name":"2018_JournalExperMed_Reversat.pdf","content_type":"application/pdf","file_size":1216437,"access_level":"open_access","date_created":"2019-02-06T08:49:52Z","creator":"dernst","date_updated":"2020-07-14T12:47:09Z","checksum":"687beea1d64c213f4cb9e3c29ec11a14","relation":"main_file"}],"volume":215,"ddc":["570"],"month":"11","has_accepted_license":"1","abstract":[{"text":"The release of IgM is the first line of an antibody response and precedes the generation of high affinity IgG in germinal centers. Once secreted by freshly activated plasmablasts, IgM is released into the efferent lymph of reactive lymph nodes as early as 3 d after immunization. As pentameric IgM has an enormous size of 1,000 kD, its diffusibility is low, and one might wonder how it can pass through the densely lymphocyte-packed environment of a lymph node parenchyma in order to reach its exit. In this issue of JEM, Thierry et al. show that, in order to reach the blood stream, IgM molecules take a specific micro-anatomical route via lymph node conduits.","lang":"eng"}],"citation":{"ama":"Reversat A, Sixt MK. IgM’s exit route. <i>Journal of Experimental Medicine</i>. 2018;215(12):2959-2961. doi:<a href=\"https://doi.org/10.1084/jem.20181934\">10.1084/jem.20181934</a>","chicago":"Reversat, Anne, and Michael K Sixt. “IgM’s Exit Route.” <i>Journal of Experimental Medicine</i>. Rockefeller University Press, 2018. <a href=\"https://doi.org/10.1084/jem.20181934\">https://doi.org/10.1084/jem.20181934</a>.","ieee":"A. Reversat and M. K. Sixt, “IgM’s exit route,” <i>Journal of Experimental Medicine</i>, vol. 215, no. 12. Rockefeller University Press, pp. 2959–2961, 2018.","short":"A. Reversat, M.K. Sixt, Journal of Experimental Medicine 215 (2018) 2959–2961.","mla":"Reversat, Anne, and Michael K. Sixt. “IgM’s Exit Route.” <i>Journal of Experimental Medicine</i>, vol. 215, no. 12, Rockefeller University Press, 2018, pp. 2959–61, doi:<a href=\"https://doi.org/10.1084/jem.20181934\">10.1084/jem.20181934</a>.","apa":"Reversat, A., &#38; Sixt, M. K. (2018). IgM’s exit route. <i>Journal of Experimental Medicine</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1084/jem.20181934\">https://doi.org/10.1084/jem.20181934</a>","ista":"Reversat A, Sixt MK. 2018. IgM’s exit route. Journal of Experimental Medicine. 215(12), 2959–2961."},"tmp":{"image":"/images/cc_by_nc_sa.png","name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"date_published":"2018-11-20T00:00:00Z","date_updated":"2026-04-16T09:54:07Z","intvolume":"       215","type":"journal_article","day":"20","external_id":{"isi":["000451920600002"]},"oa":1,"publication_status":"published","year":"2018","publisher":"Rockefeller University Press","department":[{"_id":"MiSi"}],"publication_identifier":{"issn":["0022-1007"]},"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","article_processing_charge":"No","title":"IgM's exit route","doi":"10.1084/jem.20181934","_id":"5672","scopus_import":"1","oa_version":"Published Version","language":[{"iso":"eng"}],"file_date_updated":"2020-07-14T12:47:09Z","date_created":"2018-12-16T22:59:18Z","isi":1,"quality_controlled":"1"}]