[{"researchdata_availability":"no","doi":"10.1111/nph.71072","file":[{"access_level":"open_access","relation":"main_file","creator":"dernst","success":1,"date_created":"2026-07-27T11:46:25Z","checksum":"67513dde983631bed9613fdaadbbee06","date_updated":"2026-07-27T11:46:25Z","content_type":"application/pdf","file_name":"2026_NewPhytologist_Babic.pdf","file_size":1708392,"file_id":"22424"}],"volume":250,"corr_author":"1","oa":1,"day":"01","publisher":"Wiley","department":[{"_id":"JiFr"},{"_id":"GradSch"}],"month":"05","external_id":{"pmid":["41808651"]},"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","date_published":"2026-05-01T00:00:00Z","file_date_updated":"2026-07-27T11:46:25Z","das_tickbox":"0","fulldoi":"https://doi.org/10.1111/nph.71072","ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"intvolume":"       250","publication_status":"published","pmid":1,"quality_controlled":"1","year":"2026","OA_type":"hybrid","OA_place":"publisher","language":[{"iso":"eng"}],"scopus_import":"1","issue":"3","publication":"New Phytologist","publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"acknowledgement":"The authors would like to acknowledge the many colleagues whose valuable contributions to the field could not be included in this review due to space limitations and reference constraints. Open Access funding provided by Institute of Science and Technology Austria/KEMÖ.","page":"1483-1491","citation":{"ieee":"D. Babic, M. Zupunski, and J. Friml, “Imaging and genetic toolbox to study Arabidopsis embryogenesis,” <i>New Phytologist</i>, vol. 250, no. 3. Wiley, pp. 1483–1491, 2026.","short":"D. Babic, M. Zupunski, J. Friml, New Phytologist 250 (2026) 1483–1491.","ista":"Babic D, Zupunski M, Friml J. 2026. Imaging and genetic toolbox to study Arabidopsis embryogenesis. New Phytologist. 250(3), 1483–1491.","ama":"Babic D, Zupunski M, Friml J. Imaging and genetic toolbox to study Arabidopsis embryogenesis. <i>New Phytologist</i>. 2026;250(3):1483-1491. doi:<a href=\"https://doi.org/10.1111/nph.71072\">10.1111/nph.71072</a>","mla":"Babic, David, et al. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” <i>New Phytologist</i>, vol. 250, no. 3, Wiley, 2026, pp. 1483–91, doi:<a href=\"https://doi.org/10.1111/nph.71072\">10.1111/nph.71072</a>.","apa":"Babic, D., Zupunski, M., &#38; Friml, J. (2026). Imaging and genetic toolbox to study Arabidopsis embryogenesis. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.71072\">https://doi.org/10.1111/nph.71072</a>","chicago":"Babic, David, Milan Zupunski, and Jiří Friml. “Imaging and Genetic Toolbox to Study Arabidopsis Embryogenesis.” <i>New Phytologist</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/nph.71072\">https://doi.org/10.1111/nph.71072</a>."},"PlanS_conform":"1","date_updated":"2026-07-27T11:47:24Z","supplementarymaterial":"no","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2026-03-23T14:59:06Z","author":[{"first_name":"David","last_name":"Babic","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","full_name":"Babic, David"},{"id":"f6a21fce-573e-11f0-a150-a8d96aee2539","last_name":"Zupunski","first_name":"Milan","full_name":"Zupunski, Milan"},{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"}],"status":"public","title":"Imaging and genetic toolbox to study Arabidopsis embryogenesis","_id":"21483","abstract":[{"text":"Embryogenesis in the model plant Arabidopsis thaliana provides a framework for understanding how cell polarity and patterning coordinate with hormonal signalling to establish the plant body plan. Following fertilisation, the zygote divides asymmetrically to generate apical and basal lineages, establishing the apical–basal axis that defines future shoot and root poles. Genetic and molecular analyses of classical mutants including gnom, monopteros (mp), bodenlos (bdl) and topless revealed that localised auxin biosynthesis, directional transport and downstream transcriptional responses are central to apical–basal axis establishment and organ initiation. The main components of this regulation are polarly localised PIN auxin transporters and downstream modules involving MONOPTEROS and WUSCHEL-RELATED HOMEOBOX transcription factors. Advances in microscopy have transformed the study of Arabidopsis embryogenesis: fluorescence-compatible clearing reagents and three-dimensional reconstructions now permit quantitative analyses of cell geometry, division orientation, and cytoskeletal dynamics. Live ovule imaging setups with confocal laser scanning and multiphoton microscopes enable real-time observation of embryo development, while laser-assisted cell ablation can be used to probe cell-to-cell communication and fate plasticity. Together, these methodological breakthroughs position Arabidopsis embryos as a prime model for dissecting the chemical and biophysical cues that shape plant development.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"original"},{"date_updated":"2026-08-04T07:58:27Z","supplementarymaterial":"yes","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","citation":{"apa":"Adamowski, M., Gackowski, A., Matijevic, I., Alotaibi, S. S., &#38; Friml, J. (2026). The role of clathrin in post‐Golgi secretion in plant cells. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.71454\">https://doi.org/10.1111/nph.71454</a>","chicago":"Adamowski, Maciek, Adam Gackowski, Ivana Matijevic, Saqer S. Alotaibi, and Jiří Friml. “The Role of Clathrin in Post‐Golgi Secretion in Plant Cells.” <i>New Phytologist</i>. Wiley, 2026. <a href=\"https://doi.org/10.1111/nph.71454\">https://doi.org/10.1111/nph.71454</a>.","short":"M. Adamowski, A. Gackowski, I. Matijevic, S.S. Alotaibi, J. Friml, New Phytologist (2026).","ieee":"M. Adamowski, A. Gackowski, I. Matijevic, S. S. Alotaibi, and J. Friml, “The role of clathrin in post‐Golgi secretion in plant cells,” <i>New Phytologist</i>. Wiley, 2026.","ista":"Adamowski M, Gackowski A, Matijevic I, Alotaibi SS, Friml J. 2026. The role of clathrin in post‐Golgi secretion in plant cells. New Phytologist., nph. 71454.","ama":"Adamowski M, Gackowski A, Matijevic I, Alotaibi SS, Friml J. The role of clathrin in post‐Golgi secretion in plant cells. <i>New Phytologist</i>. 2026. doi:<a href=\"https://doi.org/10.1111/nph.71454\">10.1111/nph.71454</a>","mla":"Adamowski, Maciek, et al. “The Role of Clathrin in Post‐Golgi Secretion in Plant Cells.” <i>New Phytologist</i>, nph. 71454, Wiley, 2026, doi:<a href=\"https://doi.org/10.1111/nph.71454\">10.1111/nph.71454</a>."},"project":[{"grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425"}],"abstract":[{"lang":"eng","text":"Within the plant endomembrane system, the vesicle coat protein clathrin localizes to the plasma membrane (PM) and the trans-Golgi Network/early endosome (TGN/EE). While the role of clathrin in endocytosis at the PM is well established, its function at TGN/EE, presumably in late secretion (trafficking from the TGN/EE to the cell surface) or en route to the vacuole, is debated. Similarly debated are potential homeostatic mechanisms balancing the trafficking routes, especially endocytosis and late secretion.\r\nWe address these questions in Arabidopsis thaliana using conditional silencing of CLATHRIN HEAVY CHAIN (CHC), conditional overexpression of the clathrin uncoating factor AUXILIN-LIKE1, and secretory mutants.\r\nCHC silencing interferes with trafficking of cargoes destined for the apoplast and the PM, supporting a function of clathrin in late secretion. The secretory cargoes become abnormally rerouted from the TGN/EE to the vacuole. Unlike CHC silencing, overexpression of AUXILIN-LIKE1 selectively inhibits clathrin-mediated endocytosis while secretion continues normally at early points of induction. Conversely, secretory mutants exhibit a reduced PM recruitment of clathrin, and variably, of the TPLATE endocytic component.\r\nTogether, our data show a role of clathrin in secretion and suggest secretion as a fundamental trafficking process to which endocytosis is adjusted by a weak homeostatic mechanism."}],"type":"journal_article","oa_version":"Published Version","article_type":"original","date_created":"2026-08-04T06:48:41Z","author":[{"first_name":"Maciek","last_name":"Adamowski","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","full_name":"Adamowski, Maciek","orcid":"0000-0001-6463-5257"},{"first_name":"Adam","last_name":"Gackowski","full_name":"Gackowski, Adam"},{"full_name":"Matijevic, Ivana","first_name":"Ivana","last_name":"Matijevic","id":"83c17ce3-15b2-11ec-abd3-f486545870bd"},{"full_name":"Alotaibi, Saqer S.","first_name":"Saqer S.","last_name":"Alotaibi"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří"}],"status":"public","title":"The role of clathrin in post‐Golgi secretion in plant cells","_id":"22647","language":[{"iso":"eng"}],"OA_type":"hybrid","OA_place":"publisher","acknowledgement":"The authors wish to acknowledge Dr. Paweł Baster for cloning PIN1-GFP-2/pDONR221, Ms. Aline Monzer and Dr. Mingyue Li for help with CHC protein level evaluation, Dr. Michał Rychłowski for help with confocal microscopy, Prof. Ari Pekka Mähönen for sharing the p1R4-pUBQ10:XVE plasmid, and Prof. Ying Gu for sharing seeds of the sec5 mutant. M.A. would like to thank Dr. Xixi Zhang and Prof. Sebastian Bednarek for inspiring discussions. This work was supported by the Taif University Researchers Supporting Project, TURSP-HC2022/02 to JF and SA and Austrian Science Fund (FWF): I 3630-B25 to JF. Open Access funding provided by Institute of Science and Technology Austria.","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"scopus_import":"1","publication":"New Phytologist","main_file_link":[{"url":"https://doi.org/10.1111/nph.71454","open_access":"1"}],"das_tickbox":"1","fulldoi":"https://doi.org/10.1111/nph.71454","article_processing_charge":"Yes (via OA deal)","date_published":"2026-07-20T00:00:00Z","publication_status":"epub_ahead","quality_controlled":"1","year":"2026","pmid":1,"researchdata_availability":"yes","doi":"10.1111/nph.71454","article_number":"nph.71454","publisher":"Wiley","month":"07","department":[{"_id":"JiFr"},{"_id":"MaLo"},{"_id":"GradSch"}],"external_id":{"pmid":["42477503"]},"oa":1,"corr_author":"1","day":"20","dataavailabilitystatement":"Original data associated with this study have been deposited in Dataset S1. The accession nos. of A. thaliana genes used in this study are as follows: CHC1 (AT3G11130), CHC2 (AT3G08530), CLC2 (AT2G40060), TPLATE (AT3G01780), AP2A1 (AT5G22770), DRP1C (AT1G14830), GNOM-LIKE1 (AT5G39500), BEN3/BIG2 (AT3G60860), TMK4 (AT3G23750), PIN1 (AT1G73590), AUXILIN-LIKE1 (AT4G12780), AP1M2 (AT1G60780), ECHIDNA (AT1G09330), SEC5A (AT1G76850), SEC5B (AT1G21170), TUB2 (AT5G62690), and PP2AA3 (AT1G13320)."},{"intvolume":"       246","publication_status":"published","year":"2025","quality_controlled":"1","pmid":1,"article_processing_charge":"No","date_published":"2025-04-01T00:00:00Z","fulldoi":"https://doi.org/10.1111/nph.20428","day":"01","publisher":"Wiley","department":[{"_id":"XiFe"}],"month":"04","external_id":{"isi":["001424915600001"],"pmid":["39969116"]},"isi":1,"doi":"10.1111/nph.20428","volume":246,"author":[{"first_name":"Xin","last_name":"Song","full_name":"Song, Xin"},{"full_name":"Zhang, Miao","last_name":"Zhang","first_name":"Miao"},{"first_name":"Ting Ting","last_name":"Wang","full_name":"Wang, Ting Ting"},{"full_name":"Duan, Yao Yuan","first_name":"Yao Yuan","last_name":"Duan"},{"last_name":"Ren","first_name":"Jie","full_name":"Ren, Jie"},{"first_name":"Hu","last_name":"Gao","full_name":"Gao, Hu"},{"first_name":"Yan Jie","last_name":"Fan","full_name":"Fan, Yan Jie"},{"first_name":"Qiang Ming","last_name":"Xia","full_name":"Xia, Qiang Ming"},{"first_name":"Hui Xiang","last_name":"Cao","full_name":"Cao, Hui Xiang"},{"last_name":"Xie","first_name":"Kai Dong","full_name":"Xie, Kai Dong"},{"last_name":"Wu","first_name":"Xiao Meng","full_name":"Wu, Xiao Meng"},{"last_name":"Zhang","first_name":"Fei","full_name":"Zhang, Fei"},{"full_name":"Zhang, Si Qi","first_name":"Si Qi","last_name":"Zhang"},{"last_name":"Huang","first_name":"Ying","id":"11b5bbff-8b61-11ed-b69e-d8ddd6bce951","full_name":"Huang, Ying"},{"full_name":"Boualem, Adnane","last_name":"Boualem","first_name":"Adnane"},{"last_name":"Bendahmane","first_name":"Abdelhafid","full_name":"Bendahmane, Abdelhafid"},{"full_name":"Tan, Feng Quan","first_name":"Feng Quan","last_name":"Tan"},{"first_name":"Wen Wu","last_name":"Guo","full_name":"Guo, Wen Wu"}],"date_created":"2025-03-16T23:01:25Z","status":"public","title":"Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants","_id":"19406","abstract":[{"text":"Polyploidization is a common occurrence in the evolutionary history of flowering plants, significantly contributing to their adaptability and diversity. However, the molecular mechanisms behind these adaptive advantages are not well understood.\r\nThrough comprehensive phenotyping of diploid and tetraploid clones from Citrus and Poncirus genera, we discovered that genome doubling significantly enhances salt stress resilience. Epigenetic and transcriptomic analyses revealed that increased ethylene production in the roots of tetraploid plants was associated with hypomethylation and enhanced chromatin accessibility of the ACO1 gene. This increased ethylene production activates the transcription of reactive oxygen species scavenging genes and stress-related hormone biosynthesis genes. Consequently, tetraploid plants exhibited superior root functionality under salt stress, maintaining improved cytosolic K+/Na+ homeostasis.\r\nTo genetically validate the link between salt stress resilience and ACO1 expression, we generated overexpression and knockout lines, confirming the central role of ACO1 expression regulation following genome doubling in salt stress resilience.\r\nOur work elucidates the molecular mechanisms underlying the role of genome doubling in stress resilience. We also highlight the importance of chromatin dynamics in fine-tuning ethylene gene expression and activating salt stress resilience pathways, offering valuable insights into plant adaptation and crop genome evolution.","lang":"eng"}],"type":"journal_article","oa_version":"None","article_type":"original","page":"176-191","citation":{"chicago":"Song, Xin, Miao Zhang, Ting Ting Wang, Yao Yuan Duan, Jie Ren, Hu Gao, Yan Jie Fan, et al. “Polyploidization Leads to Salt Stress Resilience via Ethylene Signaling in Citrus Plants.” <i>New Phytologist</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/nph.20428\">https://doi.org/10.1111/nph.20428</a>.","apa":"Song, X., Zhang, M., Wang, T. T., Duan, Y. Y., Ren, J., Gao, H., … Guo, W. W. (2025). Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.20428\">https://doi.org/10.1111/nph.20428</a>","ama":"Song X, Zhang M, Wang TT, et al. Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants. <i>New Phytologist</i>. 2025;246(1):176-191. doi:<a href=\"https://doi.org/10.1111/nph.20428\">10.1111/nph.20428</a>","mla":"Song, Xin, et al. “Polyploidization Leads to Salt Stress Resilience via Ethylene Signaling in Citrus Plants.” <i>New Phytologist</i>, vol. 246, no. 1, Wiley, 2025, pp. 176–91, doi:<a href=\"https://doi.org/10.1111/nph.20428\">10.1111/nph.20428</a>.","short":"X. Song, M. Zhang, T.T. Wang, Y.Y. Duan, J. Ren, H. Gao, Y.J. Fan, Q.M. Xia, H.X. Cao, K.D. Xie, X.M. Wu, F. Zhang, S.Q. Zhang, Y. Huang, A. Boualem, A. Bendahmane, F.Q. Tan, W.W. Guo, New Phytologist 246 (2025) 176–191.","ista":"Song X, Zhang M, Wang TT, Duan YY, Ren J, Gao H, Fan YJ, Xia QM, Cao HX, Xie KD, Wu XM, Zhang F, Zhang SQ, Huang Y, Boualem A, Bendahmane A, Tan FQ, Guo WW. 2025. Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants. New Phytologist. 246(1), 176–191.","ieee":"X. Song <i>et al.</i>, “Polyploidization leads to salt stress resilience via ethylene signaling in citrus plants,” <i>New Phytologist</i>, vol. 246, no. 1. Wiley, pp. 176–191, 2025."},"date_updated":"2025-09-30T11:00:06Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","scopus_import":"1","issue":"1","publication":"New Phytologist","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"acknowledgement":"We thank Prof. Qi Xie from the Institute of Genetics and Development, Chinese Academy of Sciences, for providing the YAO promoter-driven CRISPR/Cas9 vector, our colleague Dr Robert M. Larkin from Huazhong Agricultural University, and Dr Olivier Martin from IPS2 (INRAE, France) for critical reading of the manuscript. This research was financially supported by grants from the National Key Research & Development Program of China (2024YFD1200501), the National Natural Science Foundation of China (32172525 and 32202432), the Foundation of Hubei Hongshan laboratory (2021hszd009), the China Agricultural Research System (CARS-26) and the Department of Science and Technology of Hubei Province (2022BBA0019). A. Bendahmane is funded by the ANR BioAdapt (ANR-21-LCV3-0003), LabEx Saclay Plant Sciences (SPS) (ANR-10-LABX-40-SPS), and the NectarGland ERC Project (101095736).","OA_type":"closed access","language":[{"iso":"eng"}]},{"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"acknowledgement":"We gratefully acknowledge our brave colleagues, whose excellent efforts kept the plant cAMP research going in the last two decades. The authors were financially supported by the Austrian Science Fund (FWF): I 6123 and P 37051-B.","issue":"2","scopus_import":"1","publication":"New Phytologist","language":[{"iso":"eng"}],"abstract":[{"text":"The 3′,5′-cyclic adenosine monophosphate (cAMP) is a versatile second messenger in many mammalian signaling pathways. However, its role in plants remains not well-recognized. Recent discovery of adenylate cyclase (AC) activity for transport inhibitor response 1/auxin-signaling F-box proteins (TIR1/AFB) auxin receptors and the demonstration of its importance for canonical auxin signaling put plant cAMP research back into spotlight. This insight briefly summarizes the well-established cAMP signaling pathways in mammalian cells and describes the turbulent and controversial history of plant cAMP research highlighting the major progress and the unresolved points. We also briefly review the current paradigm of auxin signaling to provide a background for the discussion on the AC activity of TIR1/AFB auxin receptors and its potential role in transcriptional auxin signaling as well as impact of these discoveries on plant cAMP research in general.","lang":"eng"}],"article_type":"original","oa_version":"Published Version","type":"journal_article","date_created":"2023-07-23T22:01:13Z","author":[{"orcid":"0000-0001-5187-8401","full_name":"Qi, Linlin","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","first_name":"Linlin","last_name":"Qi"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","first_name":"Jiří"}],"status":"public","_id":"13266","title":"Tale of cAMP as a second messenger in auxin signaling and beyond","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2024-10-22T12:50:00Z","project":[{"name":"Peptide receptors for auxin canalization in Arabidopsis","_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123"},{"name":"Guanylate cyclase activity of TIR1/AFBs auxin receptors","_id":"7bcece63-9f16-11ee-852c-ae94e099eeb6","grant_number":"P37051"}],"citation":{"chicago":"Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling and Beyond.” <i>New Phytologist</i>. Wiley, 2023. <a href=\"https://doi.org/10.1111/nph.19123\">https://doi.org/10.1111/nph.19123</a>.","apa":"Qi, L., &#38; Friml, J. (2023). Tale of cAMP as a second messenger in auxin signaling and beyond. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.19123\">https://doi.org/10.1111/nph.19123</a>","ama":"Qi L, Friml J. Tale of cAMP as a second messenger in auxin signaling and beyond. <i>New Phytologist</i>. 2023;240(2):489-495. doi:<a href=\"https://doi.org/10.1111/nph.19123\">10.1111/nph.19123</a>","mla":"Qi, Linlin, and Jiří Friml. “Tale of CAMP as a Second Messenger in Auxin Signaling and Beyond.” <i>New Phytologist</i>, vol. 240, no. 2, Wiley, 2023, pp. 489–95, doi:<a href=\"https://doi.org/10.1111/nph.19123\">10.1111/nph.19123</a>.","ieee":"L. Qi and J. Friml, “Tale of cAMP as a second messenger in auxin signaling and beyond,” <i>New Phytologist</i>, vol. 240, no. 2. Wiley, pp. 489–495, 2023.","ista":"Qi L, Friml J. 2023. Tale of cAMP as a second messenger in auxin signaling and beyond. New Phytologist. 240(2), 489–495.","short":"L. Qi, J. Friml, New Phytologist 240 (2023) 489–495."},"page":"489-495","month":"10","department":[{"_id":"JiFr"}],"publisher":"Wiley","external_id":{"isi":["001026321500001"],"pmid":["37434303"]},"oa":1,"corr_author":"1","day":"01","file":[{"date_created":"2024-01-29T11:21:43Z","checksum":"6d9bbd45b8e7bb3ceee2586d447bacb2","success":1,"relation":"main_file","creator":"dernst","access_level":"open_access","file_id":"14898","file_size":974464,"content_type":"application/pdf","date_updated":"2024-01-29T11:21:43Z","file_name":"2023_NewPhytologist_Qi.pdf"}],"doi":"10.1111/nph.19123","volume":240,"isi":1,"intvolume":"       240","quality_controlled":"1","pmid":1,"year":"2023","publication_status":"published","ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"fulldoi":"https://doi.org/10.1111/nph.19123","article_processing_charge":"Yes (via OA deal)","date_published":"2023-10-01T00:00:00Z","file_date_updated":"2024-01-29T11:21:43Z","has_accepted_license":"1"},{"scopus_import":"1","issue":"1","publication":"New Phytologist","acknowledgement":"We thank Claus Schwechheimer for the pin34 and pin347 seeds, Yuliia Mironova for technical assistance, Ksenia Timofeyenko and Dmitry Konovalov for help with the evolutional analysis, Konstantin Kutashev and Siarhei Dabravolski for assistance with FRET-FLIM, Huibin Han for advice with hypocotyl imaging, Karel Müller for the initial qRT-PCR on the tobacco cell lines, Stano Pekár for suggestions regarding the statistical analysis of the morphodynamic measurements, and Jozef Mravec, Dolf Weijers and Lindy Abas for their comments on the manuscript. This work was supported by the Czech Science Foundation (projects 16-26428S and 19-23773S to IK, MH and KRůžička, 19-18917S to JHumpolíčková and 18-26981S to JF), and the Ministry of Education, Youth and Sports of the Czech Republic (MEYS, CZ.02.1.01/0.0/0.0/16_019/0000738) to KRůžička and JHejátko. The imaging facilities of the Institute of Experimental Botany and CEITEC are supported by MEYS (LM2018129 – Czech BioImaging and CZ.02.1.01/0.0/0.0/16_013/0001775). The authors declare no competing interests.","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"OA_place":"publisher","OA_type":"free access","language":[{"iso":"eng"}],"status":"public","author":[{"last_name":"Kashkan","first_name":"Ivan","full_name":"Kashkan, Ivan"},{"full_name":"Hrtyan, Mónika","id":"45A71A74-F248-11E8-B48F-1D18A9856A87","first_name":"Mónika","last_name":"Hrtyan"},{"full_name":"Retzer, Katarzyna","last_name":"Retzer","first_name":"Katarzyna"},{"full_name":"Humpolíčková, Jana","last_name":"Humpolíčková","first_name":"Jana"},{"last_name":"Jayasree","first_name":"Aswathy","full_name":"Jayasree, Aswathy"},{"last_name":"Filepová","first_name":"Roberta","full_name":"Filepová, Roberta"},{"last_name":"Vondráková","first_name":"Zuzana","full_name":"Vondráková, Zuzana"},{"id":"4542EF9A-F248-11E8-B48F-1D18A9856A87","first_name":"Sibu","last_name":"Simon","orcid":"0000-0002-1998-6741","full_name":"Simon, Sibu"},{"full_name":"Rombaut, Debbie","first_name":"Debbie","last_name":"Rombaut"},{"full_name":"Jacobs, Thomas B.","first_name":"Thomas B.","last_name":"Jacobs"},{"first_name":"Mikko J.","last_name":"Frilander","full_name":"Frilander, Mikko J."},{"full_name":"Hejátko, Jan","last_name":"Hejátko","first_name":"Jan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"first_name":"Jan","last_name":"Petrášek","full_name":"Petrášek, Jan"},{"first_name":"Kamil","last_name":"Růžička","full_name":"Růžička, Kamil"}],"date_created":"2021-11-14T23:01:24Z","title":"Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana","_id":"10282","abstract":[{"text":"Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"original","page":"329-343","citation":{"ista":"Kashkan I, Hrtyan M, Retzer K, Humpolíčková J, Jayasree A, Filepová R, Vondráková Z, Simon S, Rombaut D, Jacobs TB, Frilander MJ, Hejátko J, Friml J, Petrášek J, Růžička K. 2022. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. 233(1), 329–343.","short":"I. Kashkan, M. Hrtyan, K. Retzer, J. Humpolíčková, A. Jayasree, R. Filepová, Z. Vondráková, S. Simon, D. Rombaut, T.B. Jacobs, M.J. Frilander, J. Hejátko, J. Friml, J. Petrášek, K. Růžička, New Phytologist 233 (2022) 329–343.","ieee":"I. Kashkan <i>et al.</i>, “Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 233, no. 1. Wiley, pp. 329–343, 2022.","ama":"Kashkan I, Hrtyan M, Retzer K, et al. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. <i>New Phytologist</i>. 2022;233(1):329-343. doi:<a href=\"https://doi.org/10.1111/nph.17792\">10.1111/nph.17792</a>","mla":"Kashkan, Ivan, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 233, no. 1, Wiley, 2022, pp. 329–43, doi:<a href=\"https://doi.org/10.1111/nph.17792\">10.1111/nph.17792</a>.","apa":"Kashkan, I., Hrtyan, M., Retzer, K., Humpolíčková, J., Jayasree, A., Filepová, R., … Růžička, K. (2022). Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.17792\">https://doi.org/10.1111/nph.17792</a>","chicago":"Kashkan, Ivan, Mónika Hrtyan, Katarzyna Retzer, Jana Humpolíčková, Aswathy Jayasree, Roberta Filepová, Zuzana Vondráková, et al. “Mutually Opposing Activity of PIN7 Splicing Isoforms Is Required for Auxin-Mediated Tropic Responses in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2022. <a href=\"https://doi.org/10.1111/nph.17792\">https://doi.org/10.1111/nph.17792</a>."},"date_updated":"2026-07-28T12:06:20Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa":1,"day":"01","publisher":"Wiley","biorxivid":1,"department":[{"_id":"JiFr"}],"month":"01","external_id":{"isi":["000714678100001"],"biorxivid":["10.1101/2020.05.02.074070"],"pmid":["34637542"]},"isi":1,"doi":"10.1111/nph.17792","volume":233,"intvolume":"       233","publication_status":"published","year":"2022","quality_controlled":"1","pmid":1,"article_processing_charge":"No","date_published":"2022-01-01T00:00:00Z","main_file_link":[{"url":"https://doi.org/10.1111/nph.17792","open_access":"1"}],"fulldoi":"https://doi.org/10.1111/nph.17792"},{"publication":"New Phytologist","issue":"1","scopus_import":"1","ec_funded":1,"publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"acknowledgement":"We thank Dr Ingo Heilmann (Martin‐Luther‐University Halle‐Wittenberg) for the XVE>>PIP5K1‐YFP line, Dr Brad Day (Michigan State University) for the ndr1‐1 mutant and the complementation lines, and Dr Patricia C. Zambryski (University of California, Berkeley) for the 35S::P30‐GFP line, the Bioimaging team (IST Austria) for assistance with imaging, group members for discussions, Martine De Cock for help in preparing the manuscript and Nataliia Gnyliukh for critical reading and revision of the manuscript. This project received funding from the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program (grant agreement No. 742985) and Comisión Nacional de Investigación Científica y Tecnológica (Project CONICYT‐PAI 82130047). DvW received funding from the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007‐2013) under REA grant agreement no. 291734.","language":[{"iso":"eng"}],"_id":"8582","title":"Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana","author":[{"last_name":"Li","first_name":"Hongjiang","id":"33CA54A6-F248-11E8-B48F-1D18A9856A87","full_name":"Li, Hongjiang","orcid":"0000-0001-5039-9660"},{"first_name":"Daniel","last_name":"von Wangenheim","id":"49E91952-F248-11E8-B48F-1D18A9856A87","full_name":"von Wangenheim, Daniel","orcid":"0000-0002-6862-1247"},{"orcid":"0000-0001-7048-4627","full_name":"Zhang, Xixi","first_name":"Xixi","last_name":"Zhang","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A"},{"orcid":"0000-0002-0471-8285","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","first_name":"Shutang","last_name":"Tan"},{"orcid":"0000-0002-8821-8236","full_name":"Darwish-Miranda, Nasser","id":"39CD9926-F248-11E8-B48F-1D18A9856A87","last_name":"Darwish-Miranda","first_name":"Nasser"},{"first_name":"Satoshi","last_name":"Naramoto","full_name":"Naramoto, Satoshi"},{"full_name":"Wabnik, Krzysztof T","orcid":"0000-0001-7263-0560","last_name":"Wabnik","first_name":"Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87"},{"last_name":"de Rycke","first_name":"Riet","full_name":"de Rycke, Riet"},{"id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann","full_name":"Kaufmann, Walter","orcid":"0000-0001-9735-5315"},{"last_name":"Gütl","first_name":"Daniel J","id":"381929CE-F248-11E8-B48F-1D18A9856A87","full_name":"Gütl, Daniel J"},{"first_name":"Ricardo","last_name":"Tejos","full_name":"Tejos, Ricardo"},{"id":"399876EC-F248-11E8-B48F-1D18A9856A87","last_name":"Grones","first_name":"Peter","full_name":"Grones, Peter"},{"last_name":"Ke","first_name":"Meiyu","full_name":"Ke, Meiyu"},{"first_name":"Xu","last_name":"Chen","id":"4E5ADCAA-F248-11E8-B48F-1D18A9856A87","full_name":"Chen, Xu"},{"last_name":"Dettmer","first_name":"Jan","full_name":"Dettmer, Jan"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"}],"status":"public","date_created":"2020-09-28T08:59:28Z","article_type":"original","type":"journal_article","oa_version":"Published Version","abstract":[{"lang":"eng","text":"Cell and tissue polarization is fundamental for plant growth and morphogenesis. The polar, cellular localization of Arabidopsis PIN‐FORMED (PIN) proteins is crucial for their function in directional auxin transport. The clustering of PIN polar cargoes within the plasma membrane has been proposed to be important for the maintenance of their polar distribution. However, the more detailed features of PIN clusters and the cellular requirements of cargo clustering remain unclear.\r\nHere, we characterized PIN clusters in detail by means of multiple advanced microscopy and quantification methods, such as 3D quantitative imaging or freeze‐fracture replica labeling. The size and aggregation types of PIN clusters were determined by electron microscopy at the nanometer level at different polar domains and at different developmental stages, revealing a strong preference for clustering at the polar domains.\r\nPharmacological and genetic studies revealed that PIN clusters depend on phosphoinositol pathways, cytoskeletal structures and specific cell‐wall components as well as connections between the cell wall and the plasma membrane.\r\nThis study identifies the role of different cellular processes and structures in polar cargo clustering and provides initial mechanistic insight into the maintenance of polarity in plants and other systems."}],"project":[{"grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"grant_number":"291734","call_identifier":"FP7","name":"International IST Postdoc Fellowship Programme","_id":"25681D80-B435-11E9-9278-68D0E5697425"}],"page":"351-369","citation":{"apa":"Li, H., von Wangenheim, D., Zhang, X., Tan, S., Darwish-Miranda, N., Naramoto, S., … Friml, J. (2021). Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>","chicago":"Li, Hongjiang, Daniel von Wangenheim, Xixi Zhang, Shutang Tan, Nasser Darwish-Miranda, Satoshi Naramoto, Krzysztof T Wabnik, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16887\">https://doi.org/10.1111/nph.16887</a>.","ieee":"H. Li <i>et al.</i>, “Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana,” <i>New Phytologist</i>, vol. 229, no. 1. Wiley, pp. 351–369, 2021.","ista":"Li H, von Wangenheim D, Zhang X, Tan S, Darwish-Miranda N, Naramoto S, Wabnik KT, de Rycke R, Kaufmann W, Gütl DJ, Tejos R, Grones P, Ke M, Chen X, Dettmer J, Friml J. 2021. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. New Phytologist. 229(1), 351–369.","short":"H. Li, D. von Wangenheim, X. Zhang, S. Tan, N. Darwish-Miranda, S. Naramoto, K.T. Wabnik, R. de Rycke, W. Kaufmann, D.J. Gütl, R. Tejos, P. Grones, M. Ke, X. Chen, J. Dettmer, J. Friml, New Phytologist 229 (2021) 351–369.","mla":"Li, Hongjiang, et al. “Cellular Requirements for PIN Polar Cargo Clustering in Arabidopsis Thaliana.” <i>New Phytologist</i>, vol. 229, no. 1, Wiley, 2021, pp. 351–69, doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>.","ama":"Li H, von Wangenheim D, Zhang X, et al. Cellular requirements for PIN polar cargo clustering in Arabidopsis thaliana. <i>New Phytologist</i>. 2021;229(1):351-369. doi:<a href=\"https://doi.org/10.1111/nph.16887\">10.1111/nph.16887</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2025-06-12T06:32:24Z","day":"01","oa":1,"external_id":{"pmid":["32810889"],"isi":["000570187900001"]},"department":[{"_id":"JiFr"},{"_id":"EM-Fac"},{"_id":"Bio"},{"_id":"EvBe"}],"month":"01","publisher":"Wiley","isi":1,"volume":229,"file":[{"success":1,"date_created":"2021-02-04T09:44:17Z","checksum":"b45621607b4cab97eeb1605ab58e896e","access_level":"open_access","creator":"dernst","relation":"main_file","file_id":"9084","date_updated":"2021-02-04T09:44:17Z","content_type":"application/pdf","file_name":"2021_NewPhytologist_Li.pdf","file_size":4061962}],"doi":"10.1111/nph.16887","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["580"],"year":"2021","quality_controlled":"1","pmid":1,"publication_status":"published","intvolume":"       229","date_published":"2021-01-01T00:00:00Z","file_date_updated":"2021-02-04T09:44:17Z","has_accepted_license":"1","article_processing_charge":"Yes (via OA deal)","fulldoi":"https://doi.org/10.1111/nph.16887","acknowledged_ssus":[{"_id":"Bio"}]},{"publication":"New Phytologist","issue":"5","scopus_import":"1","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"OA_type":"free access","OA_place":"publisher","language":[{"iso":"eng"}],"_id":"22570","title":"Integrating the evidence for a terrestrial carbon sink caused by increasing atmospheric CO2","date_created":"2026-07-27T12:30:24Z","status":"public","author":[{"first_name":"Anthony P.","last_name":"Walker","full_name":"Walker, Anthony P."},{"full_name":"De Kauwe, Martin G.","first_name":"Martin G.","last_name":"De Kauwe"},{"full_name":"Bastos, Ana","first_name":"Ana","last_name":"Bastos"},{"first_name":"Soumaya","last_name":"Belmecheri","full_name":"Belmecheri, Soumaya"},{"first_name":"Katerina","last_name":"Georgiou","full_name":"Georgiou, Katerina"},{"first_name":"Ralph F.","last_name":"Keeling","full_name":"Keeling, Ralph F."},{"first_name":"Sean M.","last_name":"McMahon","full_name":"McMahon, Sean M."},{"last_name":"Medlyn","first_name":"Belinda E.","full_name":"Medlyn, Belinda E."},{"full_name":"Moore, David J. P.","last_name":"Moore","first_name":"David J. P."},{"last_name":"Norby","first_name":"Richard J.","full_name":"Norby, Richard J."},{"full_name":"Zaehle, Sönke","first_name":"Sönke","last_name":"Zaehle"},{"full_name":"Anderson‐Teixeira, Kristina J.","last_name":"Anderson‐Teixeira","first_name":"Kristina J."},{"full_name":"Battipaglia, Giovanna","first_name":"Giovanna","last_name":"Battipaglia"},{"last_name":"Brienen","first_name":"Roel J. W.","full_name":"Brienen, Roel J. W."},{"full_name":"Cabugao, Kristine G.","first_name":"Kristine G.","last_name":"Cabugao"},{"full_name":"Cailleret, Maxime","first_name":"Maxime","last_name":"Cailleret"},{"first_name":"Elliott","last_name":"Campbell","full_name":"Campbell, Elliott"},{"last_name":"Canadell","first_name":"Josep G.","full_name":"Canadell, Josep G."},{"full_name":"Ciais, Philippe","first_name":"Philippe","last_name":"Ciais"},{"full_name":"Craig, Matthew E.","last_name":"Craig","first_name":"Matthew E."},{"first_name":"David S.","last_name":"Ellsworth","full_name":"Ellsworth, David S."},{"full_name":"Farquhar, Graham D.","first_name":"Graham D.","last_name":"Farquhar"},{"full_name":"Fatichi, Simone","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6","last_name":"Fatichi","first_name":"Simone"},{"first_name":"Joshua B.","last_name":"Fisher","full_name":"Fisher, Joshua B."},{"last_name":"Frank","first_name":"David C.","full_name":"Frank, David C."},{"full_name":"Graven, Heather","first_name":"Heather","last_name":"Graven"},{"full_name":"Gu, Lianhong","first_name":"Lianhong","last_name":"Gu"},{"first_name":"Vanessa","last_name":"Haverd","full_name":"Haverd, Vanessa"},{"first_name":"Kelly","last_name":"Heilman","full_name":"Heilman, Kelly"},{"last_name":"Heimann","first_name":"Martin","full_name":"Heimann, Martin"},{"full_name":"Hungate, Bruce A.","first_name":"Bruce A.","last_name":"Hungate"},{"full_name":"Iversen, Colleen M.","last_name":"Iversen","first_name":"Colleen M."},{"full_name":"Joos, Fortunat","last_name":"Joos","first_name":"Fortunat"},{"full_name":"Jiang, Mingkai","last_name":"Jiang","first_name":"Mingkai"},{"full_name":"Keenan, Trevor F.","last_name":"Keenan","first_name":"Trevor F."},{"full_name":"Knauer, Jürgen","last_name":"Knauer","first_name":"Jürgen"},{"last_name":"Körner","first_name":"Christian","full_name":"Körner, Christian"},{"full_name":"Leshyk, Victor O.","first_name":"Victor O.","last_name":"Leshyk"},{"full_name":"Leuzinger, Sebastian","first_name":"Sebastian","last_name":"Leuzinger"},{"full_name":"Liu, Yao","first_name":"Yao","last_name":"Liu"},{"first_name":"Natasha","last_name":"MacBean","full_name":"MacBean, Natasha"},{"full_name":"Malhi, Yadvinder","last_name":"Malhi","first_name":"Yadvinder"},{"full_name":"McVicar, Tim R.","last_name":"McVicar","first_name":"Tim R."},{"last_name":"Penuelas","first_name":"Josep","full_name":"Penuelas, Josep"},{"full_name":"Pongratz, Julia","last_name":"Pongratz","first_name":"Julia"},{"first_name":"A. Shafer","last_name":"Powell","full_name":"Powell, A. Shafer"},{"full_name":"Riutta, Terhi","last_name":"Riutta","first_name":"Terhi"},{"last_name":"Sabot","first_name":"Manon E. B.","full_name":"Sabot, Manon E. B."},{"last_name":"Schleucher","first_name":"Juergen","full_name":"Schleucher, Juergen"},{"full_name":"Sitch, Stephen","first_name":"Stephen","last_name":"Sitch"},{"first_name":"William K.","last_name":"Smith","full_name":"Smith, William K."},{"full_name":"Sulman, Benjamin","last_name":"Sulman","first_name":"Benjamin"},{"first_name":"Benton","last_name":"Taylor","full_name":"Taylor, Benton"},{"last_name":"Terrer","first_name":"César","full_name":"Terrer, César"},{"full_name":"Torn, Margaret S.","first_name":"Margaret S.","last_name":"Torn"},{"full_name":"Treseder, Kathleen K.","last_name":"Treseder","first_name":"Kathleen K."},{"last_name":"Trugman","first_name":"Anna T.","full_name":"Trugman, Anna T."},{"full_name":"Trumbore, Susan E.","first_name":"Susan E.","last_name":"Trumbore"},{"last_name":"van Mantgem","first_name":"Phillip J.","full_name":"van Mantgem, Phillip J."},{"full_name":"Voelker, Steve L.","first_name":"Steve L.","last_name":"Voelker"},{"full_name":"Whelan, Mary E.","first_name":"Mary E.","last_name":"Whelan"},{"full_name":"Zuidema, Pieter A.","first_name":"Pieter A.","last_name":"Zuidema"}],"article_type":"original","type":"journal_article","oa_version":"Published Version","abstract":[{"text":"Atmospheric carbon dioxide concentration ([CO 2 ]) is increasing, which increases leaf-scalephotosynthesis and intrinsic water-use efficiency. These direct responses have the potential toincrease plant growth, vegetation biomass, and soil organic matter; transferring carbon from theatmosphere into terrestrial ecosystems (a carbon sink). A substantial global terrestrial carbon sinkwould slow the rate of [CO 2] increase and thus climate change. However, ecosystem CO2responses are complex or confounded by concurrent changes in multiple agents of global changeand evidence for a [CO 2]-driven terrestrial carbon sink can appear contradictory. Here wesynthesize theory and broad, multidisciplinary evidence for the effects of increasing [CO 2](iCO 2) on the global terrestrial carbon sink. Evidence suggests a substantial increase in globalphotosynthesis since pre-industrial times. Established theory, supported by experiments,indicates that iCO 2 is likely responsible for about half of the increase. Global carbon budgeting,atmospheric data, and forest inventories indicate a historical carbon sink, and these apparentiCO 2 responses are high in comparison to experiments and predictions from theory. Plantmortality and soil carbon iCO 2 responses are highly uncertain. In conclusion, a range of evidencesupports a positive terrestrial carbon sink in response to iCO2 , albeit with uncertain magnitudeand strong suggestion of a role for additional agents of global change.","lang":"eng"}],"extern":"1","citation":{"mla":"Walker, Anthony P., et al. “Integrating the Evidence for a Terrestrial Carbon Sink Caused by Increasing Atmospheric CO2.” <i>New Phytologist</i>, vol. 229, no. 5, Wiley, 2021, pp. 2413–45, doi:<a href=\"https://doi.org/10.1111/nph.16866\">10.1111/nph.16866</a>.","ama":"Walker AP, De Kauwe MG, Bastos A, et al. Integrating the evidence for a terrestrial carbon sink caused by increasing atmospheric CO2. <i>New Phytologist</i>. 2021;229(5):2413-2445. doi:<a href=\"https://doi.org/10.1111/nph.16866\">10.1111/nph.16866</a>","ieee":"A. P. Walker <i>et al.</i>, “Integrating the evidence for a terrestrial carbon sink caused by increasing atmospheric CO2,” <i>New Phytologist</i>, vol. 229, no. 5. Wiley, pp. 2413–2445, 2021.","ista":"Walker AP, De Kauwe MG, Bastos A, Belmecheri S, Georgiou K, Keeling RF, McMahon SM, Medlyn BE, Moore DJP, Norby RJ, Zaehle S, Anderson‐Teixeira KJ, Battipaglia G, Brienen RJW, Cabugao KG, Cailleret M, Campbell E, Canadell JG, Ciais P, Craig ME, Ellsworth DS, Farquhar GD, Fatichi S, Fisher JB, Frank DC, Graven H, Gu L, Haverd V, Heilman K, Heimann M, Hungate BA, Iversen CM, Joos F, Jiang M, Keenan TF, Knauer J, Körner C, Leshyk VO, Leuzinger S, Liu Y, MacBean N, Malhi Y, McVicar TR, Penuelas J, Pongratz J, Powell AS, Riutta T, Sabot MEB, Schleucher J, Sitch S, Smith WK, Sulman B, Taylor B, Terrer C, Torn MS, Treseder KK, Trugman AT, Trumbore SE, van Mantgem PJ, Voelker SL, Whelan ME, Zuidema PA. 2021. Integrating the evidence for a terrestrial carbon sink caused by increasing atmospheric CO2. New Phytologist. 229(5), 2413–2445.","short":"A.P. Walker, M.G. De Kauwe, A. Bastos, S. Belmecheri, K. Georgiou, R.F. Keeling, S.M. McMahon, B.E. Medlyn, D.J.P. Moore, R.J. Norby, S. Zaehle, K.J. Anderson‐Teixeira, G. Battipaglia, R.J.W. Brienen, K.G. Cabugao, M. Cailleret, E. Campbell, J.G. Canadell, P. Ciais, M.E. Craig, D.S. Ellsworth, G.D. Farquhar, S. Fatichi, J.B. Fisher, D.C. Frank, H. Graven, L. Gu, V. Haverd, K. Heilman, M. Heimann, B.A. Hungate, C.M. Iversen, F. Joos, M. Jiang, T.F. Keenan, J. Knauer, C. Körner, V.O. Leshyk, S. Leuzinger, Y. Liu, N. MacBean, Y. Malhi, T.R. McVicar, J. Penuelas, J. Pongratz, A.S. Powell, T. Riutta, M.E.B. Sabot, J. Schleucher, S. Sitch, W.K. Smith, B. Sulman, B. Taylor, C. Terrer, M.S. Torn, K.K. Treseder, A.T. Trugman, S.E. Trumbore, P.J. van Mantgem, S.L. Voelker, M.E. Whelan, P.A. Zuidema, New Phytologist 229 (2021) 2413–2445.","chicago":"Walker, Anthony P., Martin G. De Kauwe, Ana Bastos, Soumaya Belmecheri, Katerina Georgiou, Ralph F. Keeling, Sean M. McMahon, et al. “Integrating the Evidence for a Terrestrial Carbon Sink Caused by Increasing Atmospheric CO2.” <i>New Phytologist</i>. Wiley, 2021. <a href=\"https://doi.org/10.1111/nph.16866\">https://doi.org/10.1111/nph.16866</a>.","apa":"Walker, A. P., De Kauwe, M. G., Bastos, A., Belmecheri, S., Georgiou, K., Keeling, R. F., … Zuidema, P. A. (2021). Integrating the evidence for a terrestrial carbon sink caused by increasing atmospheric CO2. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16866\">https://doi.org/10.1111/nph.16866</a>"},"page":"2413-2445","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_updated":"2026-08-06T08:39:39Z","day":"01","oa":1,"external_id":{"pmid":["32789857"]},"month":"03","publisher":"Wiley","volume":229,"doi":"10.1111/nph.16866","year":"2021","quality_controlled":"1","pmid":1,"publication_status":"published","intvolume":"       229","date_published":"2021-03-01T00:00:00Z","keyword":["Beta factor","Carbon dioxide","CO2 fertilization","CO2-fertilization hypothesis","Free-air CO2 enrichment (FACE)","Global carbon cycle","Land–atmosphere feedback","Terrestrial ecosystems"],"article_processing_charge":"No","fulldoi":"https://doi.org/10.1111/nph.16866","das_tickbox":"1","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/nph.16866"}]},{"author":[{"orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","last_name":"Zhang","first_name":"Yuzhou"},{"id":"AEFB2266-8ABF-11EA-AA39-812C3623CBE4","last_name":"Hartinger","first_name":"Corinna","orcid":"0000-0003-1618-2737","full_name":"Hartinger, Corinna"},{"first_name":"Xiaojuan","last_name":"Wang","full_name":"Wang, Xiaojuan"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"date_created":"2020-04-30T08:43:29Z","status":"public","_id":"7697","title":"Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters","abstract":[{"text":"* Morphogenesis and adaptive tropic growth in plants depend on gradients of the phytohormone auxin, mediated by the membrane‐based PIN‐FORMED (PIN) auxin transporters. PINs localize to a particular side of the plasma membrane (PM) or to the endoplasmic reticulum (ER) to directionally transport auxin and maintain intercellular and intracellular auxin homeostasis, respectively. However, the molecular cues that confer their diverse cellular localizations remain largely unknown.\r\n* In this study, we systematically swapped the domains between ER‐ and PM‐localized PIN proteins, as well as between apical and basal PM‐localized PINs from Arabidopsis thaliana , to shed light on why PIN family members with similar topological structures reside at different membrane compartments within cells.\r\n* Our results show that not only do the N‐ and C‐terminal transmembrane domains (TMDs) and central hydrophilic loop contribute to their differential subcellular localizations and cellular polarity, but that the pairwise‐matched N‐ and C‐terminal TMDs resulting from intramolecular domain–domain coevolution are also crucial for their divergent patterns of localization.\r\n* These findings illustrate the complexity of the evolutionary path of PIN proteins in acquiring their plethora of developmental functions and adaptive growth in plants.","lang":"eng"}],"article_type":"original","type":"journal_article","oa_version":"Published Version","project":[{"grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630"},{"grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme","call_identifier":"FP7"}],"citation":{"ieee":"Y. Zhang, C. Hartinger, X. Wang, and J. Friml, “Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters,” <i>New Phytologist</i>, vol. 227, no. 5. Wiley, pp. 1406–1416, 2020.","short":"Y. Zhang, C. Hartinger, X. Wang, J. Friml, New Phytologist 227 (2020) 1406–1416.","ista":"Zhang Y, Hartinger C, Wang X, Friml J. 2020. Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. New Phytologist. 227(5), 1406–1416.","mla":"Zhang, Yuzhou, et al. “Directional Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin Transporters.” <i>New Phytologist</i>, vol. 227, no. 5, Wiley, 2020, pp. 1406–16, doi:<a href=\"https://doi.org/10.1111/nph.16629\">10.1111/nph.16629</a>.","ama":"Zhang Y, Hartinger C, Wang X, Friml J. Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. <i>New Phytologist</i>. 2020;227(5):1406-1416. doi:<a href=\"https://doi.org/10.1111/nph.16629\">10.1111/nph.16629</a>","apa":"Zhang, Y., Hartinger, C., Wang, X., &#38; Friml, J. (2020). Directional auxin fluxes in plants by intramolecular domain‐domain co‐evolution of PIN auxin transporters. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16629\">https://doi.org/10.1111/nph.16629</a>","chicago":"Zhang, Yuzhou, Corinna Hartinger, Xiaojuan Wang, and Jiří Friml. “Directional Auxin Fluxes in Plants by Intramolecular Domain‐domain Co‐evolution of PIN Auxin Transporters.” <i>New Phytologist</i>. Wiley, 2020. <a href=\"https://doi.org/10.1111/nph.16629\">https://doi.org/10.1111/nph.16629</a>."},"page":"1406-1416","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_updated":"2025-04-14T07:45:03Z","issue":"5","scopus_import":"1","publication":"New Phytologist","publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"ec_funded":1,"language":[{"iso":"eng"}],"ddc":["580"],"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"intvolume":"       227","pmid":1,"quality_controlled":"1","year":"2020","publication_status":"published","article_processing_charge":"Yes (via OA deal)","file_date_updated":"2020-11-24T12:19:38Z","date_published":"2020-09-01T00:00:00Z","has_accepted_license":"1","fulldoi":"https://doi.org/10.1111/nph.16629","corr_author":"1","oa":1,"day":"01","department":[{"_id":"JiFr"}],"month":"09","publisher":"Wiley","external_id":{"isi":["000534092400001"],"pmid":["32350870"]},"isi":1,"file":[{"file_id":"8799","content_type":"application/pdf","date_updated":"2020-11-24T12:19:38Z","file_name":"2020_09_NewPhytologist_Zhang.pdf","file_size":3643395,"success":1,"date_created":"2020-11-24T12:19:38Z","checksum":"8e8150dbbba8cb65b72f81d1f0864b8b","access_level":"open_access","relation":"main_file","creator":"dernst"}],"doi":"10.1111/nph.16629","volume":227},{"doi":"10.1111/nph.16180","file":[{"access_level":"open_access","relation":"main_file","creator":"dernst","date_created":"2019-11-13T08:15:05Z","checksum":"21e4c95599bbcaf7c483b89954658672","file_name":"2019_NewPhytologist_Pickup.pdf","date_updated":"2020-07-14T12:47:42Z","content_type":"application/pdf","file_size":1511958,"file_id":"7011"}],"volume":224,"oa":1,"day":"01","publisher":"Wiley","month":"11","department":[{"_id":"NiBa"}],"external_id":{"pmid":["31505037"]},"article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","date_published":"2019-11-01T00:00:00Z","file_date_updated":"2020-07-14T12:47:42Z","fulldoi":"https://doi.org/10.1111/nph.16180","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"ddc":["570"],"intvolume":"       224","publication_status":"published","pmid":1,"year":"2019","quality_controlled":"1","OA_place":"publisher","OA_type":"hybrid","language":[{"iso":"eng"}],"scopus_import":"1","issue":"3","publication":"New Phytologist","publication_identifier":{"issn":["0028-646X"],"eissn":["1469-8137"]},"ec_funded":1,"page":"1035-1047","citation":{"chicago":"Pickup, Melinda, Nicholas H Barton, Yaniv Brandvain, Christelle Fraisse, Sarah Yakimowski, Tanmay Dixit, Christian Lexer, Eva Cereghetti, and David Field. “Mating System Variation in Hybrid Zones: Facilitation, Barriers and Asymmetries to Gene Flow.” <i>New Phytologist</i>. Wiley, 2019. <a href=\"https://doi.org/10.1111/nph.16180\">https://doi.org/10.1111/nph.16180</a>.","apa":"Pickup, M., Barton, N. H., Brandvain, Y., Fraisse, C., Yakimowski, S., Dixit, T., … Field, D. (2019). Mating system variation in hybrid zones: Facilitation, barriers and asymmetries to gene flow. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.16180\">https://doi.org/10.1111/nph.16180</a>","mla":"Pickup, Melinda, et al. “Mating System Variation in Hybrid Zones: Facilitation, Barriers and Asymmetries to Gene Flow.” <i>New Phytologist</i>, vol. 224, no. 3, Wiley, 2019, pp. 1035–47, doi:<a href=\"https://doi.org/10.1111/nph.16180\">10.1111/nph.16180</a>.","ama":"Pickup M, Barton NH, Brandvain Y, et al. Mating system variation in hybrid zones: Facilitation, barriers and asymmetries to gene flow. <i>New Phytologist</i>. 2019;224(3):1035-1047. doi:<a href=\"https://doi.org/10.1111/nph.16180\">10.1111/nph.16180</a>","ieee":"M. Pickup <i>et al.</i>, “Mating system variation in hybrid zones: Facilitation, barriers and asymmetries to gene flow,” <i>New Phytologist</i>, vol. 224, no. 3. Wiley, pp. 1035–1047, 2019.","short":"M. Pickup, N.H. Barton, Y. Brandvain, C. Fraisse, S. Yakimowski, T. Dixit, C. Lexer, E. Cereghetti, D. Field, New Phytologist 224 (2019) 1035–1047.","ista":"Pickup M, Barton NH, Brandvain Y, Fraisse C, Yakimowski S, Dixit T, Lexer C, Cereghetti E, Field D. 2019. Mating system variation in hybrid zones: Facilitation, barriers and asymmetries to gene flow. New Phytologist. 224(3), 1035–1047."},"project":[{"grant_number":"329960","_id":"25B36484-B435-11E9-9278-68D0E5697425","name":"Mating system and the evolutionary dynamics of hybrid zones","call_identifier":"FP7"},{"grant_number":"M02463","call_identifier":"FWF","name":"Sex chromosomes and species barriers","_id":"2662AADE-B435-11E9-9278-68D0E5697425"}],"date_updated":"2026-07-28T13:22:14Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2019-09-07T14:35:40Z","author":[{"orcid":"0000-0001-6118-0541","full_name":"Pickup, Melinda","id":"2C78037E-F248-11E8-B48F-1D18A9856A87","last_name":"Pickup","first_name":"Melinda"},{"full_name":"Barton, Nicholas H","orcid":"0000-0002-8548-5240","first_name":"Nicholas H","last_name":"Barton","id":"4880FE40-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Yaniv","last_name":"Brandvain","full_name":"Brandvain, Yaniv"},{"full_name":"Fraisse, Christelle","orcid":"0000-0001-8441-5075","id":"32DF5794-F248-11E8-B48F-1D18A9856A87","last_name":"Fraisse","first_name":"Christelle"},{"full_name":"Yakimowski, Sarah","last_name":"Yakimowski","first_name":"Sarah"},{"full_name":"Dixit, Tanmay","first_name":"Tanmay","last_name":"Dixit"},{"full_name":"Lexer, Christian","first_name":"Christian","last_name":"Lexer"},{"last_name":"Cereghetti","first_name":"Eva","id":"71AA91B4-05ED-11EA-8BEB-F5833E63BD63","full_name":"Cereghetti, Eva"},{"orcid":"0000-0002-4014-8478","full_name":"Field, David","id":"419049E2-F248-11E8-B48F-1D18A9856A87","last_name":"Field","first_name":"David"}],"status":"public","title":"Mating system variation in hybrid zones: Facilitation, barriers and asymmetries to gene flow","_id":"6856","abstract":[{"text":"Plant mating systems play a key role in structuring genetic variation both within and between species. In hybrid zones, the outcomes and dynamics of hybridization are usually interpreted as the balance between gene flow and selection against hybrids. Yet, mating systems can introduce selective forces that alter these expectations; with diverse outcomes for the level and direction of gene flow depending on variation in outcrossing and whether the mating systems of the species pair are the same or divergent. We present a survey of hybridization in 133 species pairs from 41 plant families and examine how patterns of hybridization vary with mating system. We examine if hybrid zone mode, level of gene flow, asymmetries in gene flow and the frequency of reproductive isolating barriers vary in relation to mating system/s of the species pair. We combine these results with a simulation model and examples from the literature to address two general themes: (i) the two‐way interaction between introgression and the evolution of reproductive systems, and (ii) how mating system can facilitate or restrict interspecific gene flow. We conclude that examining mating system with hybridization provides unique opportunities to understand divergence and the processes underlying reproductive isolation.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"original"},{"intvolume":"       221","publication_status":"published","year":"2019","pmid":1,"quality_controlled":"1","main_file_link":[{"url":"https://doi.org/10.1111/nph.15451","open_access":"1"}],"das_tickbox":"1","fulldoi":"https://doi.org/10.1111/nph.15451","article_processing_charge":"No","keyword":["Ecosystem modelling","Nonstructural carbohydrates","Carbon cycle","Photosynthesis","Plant growth","respiration"],"date_published":"2019-01-01T00:00:00Z","publisher":"Wiley","month":"01","external_id":{"pmid":["30339280"]},"oa":1,"day":"01","doi":"10.1111/nph.15451","volume":221,"extern":"1","abstract":[{"text":"The increase in atmospheric CO2 in the future is one of the most certain projections in environmental sciences. Understanding whether vegetation carbon assimilation, growth, and changes in vegetation carbon stocks are affected by higher atmospheric CO2 and translating this understanding in mechanistic vegetation models is of utmost importance. This is highlighted by inconsistencies between global-scale studies that attribute terrestrial carbon sinks to CO2 stimulation of gross and net primary production on the one hand, and forest inventories, tree-scale studies, and plant physiological evidence showing a much less pronounced CO2 fertilization effect on the other hand. Here, we review how plant carbon sources and sinks are currently described in terrestrial biosphere models. We highlight an uneven representation of complexity between the modelling of photosynthesis and other processes, such as plant respiration, direct carbon sinks, and carbon allocation, largely driven by available observations. Despite a general lack of data on carbon sink dynamics to drive model improvements, ways forward toward a mechanistic representation of plant carbon sinks are discussed, leveraging on results obtained from plant-scale models and on observations geared toward model developments.","lang":"eng"}],"oa_version":"Published Version","type":"journal_article","article_type":"review","status":"public","author":[{"full_name":"Fatichi, Simone","first_name":"Simone","last_name":"Fatichi","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6"},{"full_name":"Pappas, Christoforos","last_name":"Pappas","first_name":"Christoforos"},{"full_name":"Zscheischler, Jakob","last_name":"Zscheischler","first_name":"Jakob"},{"full_name":"Leuzinger, Sebastian","last_name":"Leuzinger","first_name":"Sebastian"}],"date_created":"2026-07-27T12:30:24Z","title":"Modelling carbon sources and sinks in terrestrial vegetation","_id":"22522","date_updated":"2026-08-06T07:39:23Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","page":"652-668","citation":{"ieee":"S. Fatichi, C. Pappas, J. Zscheischler, and S. Leuzinger, “Modelling carbon sources and sinks in terrestrial vegetation,” <i>New Phytologist</i>, vol. 221, no. 2. Wiley, pp. 652–668, 2019.","ista":"Fatichi S, Pappas C, Zscheischler J, Leuzinger S. 2019. Modelling carbon sources and sinks in terrestrial vegetation. New Phytologist. 221(2), 652–668.","short":"S. Fatichi, C. Pappas, J. Zscheischler, S. Leuzinger, New Phytologist 221 (2019) 652–668.","ama":"Fatichi S, Pappas C, Zscheischler J, Leuzinger S. Modelling carbon sources and sinks in terrestrial vegetation. <i>New Phytologist</i>. 2019;221(2):652-668. doi:<a href=\"https://doi.org/10.1111/nph.15451\">10.1111/nph.15451</a>","mla":"Fatichi, Simone, et al. “Modelling Carbon Sources and Sinks in Terrestrial Vegetation.” <i>New Phytologist</i>, vol. 221, no. 2, Wiley, 2019, pp. 652–68, doi:<a href=\"https://doi.org/10.1111/nph.15451\">10.1111/nph.15451</a>.","apa":"Fatichi, S., Pappas, C., Zscheischler, J., &#38; Leuzinger, S. (2019). Modelling carbon sources and sinks in terrestrial vegetation. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.15451\">https://doi.org/10.1111/nph.15451</a>","chicago":"Fatichi, Simone, Christoforos Pappas, Jakob Zscheischler, and Sebastian Leuzinger. “Modelling Carbon Sources and Sinks in Terrestrial Vegetation.” <i>New Phytologist</i>. Wiley, 2019. <a href=\"https://doi.org/10.1111/nph.15451\">https://doi.org/10.1111/nph.15451</a>."},"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"scopus_import":"1","issue":"2","publication":"New Phytologist","language":[{"iso":"eng"}],"OA_type":"free access","OA_place":"publisher"},{"citation":{"ama":"Pappas C, Fatichi S, Burlando P. Modeling terrestrial carbon and water dynamics across climatic gradients: does plant trait diversity matter? <i>New Phytologist</i>. 2016;209(1):137-151. doi:<a href=\"https://doi.org/10.1111/nph.13590\">10.1111/nph.13590</a>","mla":"Pappas, Christoforos, et al. “Modeling Terrestrial Carbon and Water Dynamics across Climatic Gradients: Does Plant Trait Diversity Matter?” <i>New Phytologist</i>, vol. 209, no. 1, Wiley, 2016, pp. 137–51, doi:<a href=\"https://doi.org/10.1111/nph.13590\">10.1111/nph.13590</a>.","short":"C. Pappas, S. Fatichi, P. Burlando, New Phytologist 209 (2016) 137–151.","ieee":"C. Pappas, S. Fatichi, and P. Burlando, “Modeling terrestrial carbon and water dynamics across climatic gradients: does plant trait diversity matter?,” <i>New Phytologist</i>, vol. 209, no. 1. Wiley, pp. 137–151, 2016.","ista":"Pappas C, Fatichi S, Burlando P. 2016. Modeling terrestrial carbon and water dynamics across climatic gradients: does plant trait diversity matter? New Phytologist. 209(1), 137–151.","chicago":"Pappas, Christoforos, Simone Fatichi, and Paolo Burlando. “Modeling Terrestrial Carbon and Water Dynamics across Climatic Gradients: Does Plant Trait Diversity Matter?” <i>New Phytologist</i>. Wiley, 2016. <a href=\"https://doi.org/10.1111/nph.13590\">https://doi.org/10.1111/nph.13590</a>.","apa":"Pappas, C., Fatichi, S., &#38; Burlando, P. (2016). Modeling terrestrial carbon and water dynamics across climatic gradients: does plant trait diversity matter? <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.13590\">https://doi.org/10.1111/nph.13590</a>"},"page":"137-151","date_updated":"2026-08-03T14:15:57Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_created":"2026-07-27T12:30:24Z","status":"public","author":[{"first_name":"Christoforos","last_name":"Pappas","full_name":"Pappas, Christoforos"},{"full_name":"Fatichi, Simone","last_name":"Fatichi","first_name":"Simone","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6"},{"last_name":"Burlando","first_name":"Paolo","full_name":"Burlando, Paolo"}],"title":"Modeling terrestrial carbon and water dynamics across climatic gradients: does plant trait diversity matter?","_id":"22503","extern":"1","abstract":[{"text":"Plant trait diversity in many vegetation models is crudely represented using a discrete classification of a handful of ‘plant types’ (named plant functional types; PFTs). The parameterization of PFTs reflects mean properties of observed plant traits over broad categories ignoring most of the inter‐ and intraspecific plant trait variability.\r\n\r\nTaking advantage of a multivariate leaf‐trait distribution (leaf economics spectrum), as well as documented plant drought strategies, we generate an ensemble of hypothetical species with coordinated attributes, rather than using few PFTs. The behavior of these proxy species is tested using a mechanistic ecohydrological model that translates plant traits into plant performance. Simulations are carried out for a range of climates representative of different elevations and wetness conditions in the European Alps. Using this framework we investigate the sensitivity of ecosystem response to plant trait diversity and compare it with the sensitivity to climate variability.\r\n\r\nPlant trait diversity leads to highly divergent vegetation carbon dynamics (fluxes and pools) and to a lesser extent water fluxes (transpiration). Abiotic variables, such as soil water content and evaporation, are only marginally affected.\r\n\r\nThese results highlight the need for revising the representation of plant attributes in vegetation models. Probabilistic approaches, based on observed multivariate whole‐plant trait distributions, provide a viable alternative.","lang":"eng"}],"type":"journal_article","oa_version":"Published Version","article_type":"original","OA_place":"publisher","OA_type":"free access","language":[{"iso":"eng"}],"scopus_import":"1","issue":"1","publication":"New Phytologist","publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"article_processing_charge":"No","keyword":["aggregation biases","carbon andwater dynamics","fallacy of averages","inter-and intraspecific plant trait diversity","plantfunctional types (PFTs)","plant trait spectra","vegetation modeling"],"date_published":"2016-01-01T00:00:00Z","main_file_link":[{"url":" https://doi.org/10.1111/nph.13590","open_access":"1"}],"das_tickbox":"1","fulldoi":"https://doi.org/10.1111/nph.13590","intvolume":"       209","publication_status":"published","pmid":1,"year":"2016","quality_controlled":"1","doi":"10.1111/nph.13590","volume":209,"oa":1,"day":"01","publisher":"Wiley","month":"01","external_id":{"pmid":["26389742"]}},{"quality_controlled":"1","year":"2014","pmid":1,"publication_status":"published","intvolume":"       201","fulldoi":"https://doi.org/10.1111/nph.12614","das_tickbox":"1","main_file_link":[{"url":"https://doi.org/10.1111/nph.12614","open_access":"1"}],"date_published":"2014-03-01T00:00:00Z","article_processing_charge":"No","external_id":{"pmid":["24261587"]},"month":"03","publisher":"Wiley","day":"01","oa":1,"volume":201,"doi":"10.1111/nph.12614","article_type":"letter_note","type":"journal_article","oa_version":"Published Version","extern":"1","_id":"22541","title":"Moving beyond photosynthesis: from carbon source to sink‐driven vegetation modeling","date_created":"2026-07-27T12:30:24Z","status":"public","author":[{"first_name":"Simone","last_name":"Fatichi","id":"cf8e546b-a9b0-11f0-a43b-aa89ed1b56d6","full_name":"Fatichi, Simone"},{"first_name":"Sebastian","last_name":"Leuzinger","full_name":"Leuzinger, Sebastian"},{"full_name":"Körner, Christian","last_name":"Körner","first_name":"Christian"}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_updated":"2026-08-06T08:12:29Z","page":"1086-1095","citation":{"chicago":"Fatichi, Simone, Sebastian Leuzinger, and Christian Körner. “Moving beyond Photosynthesis: From Carbon Source to Sink‐driven Vegetation Modeling.” <i>New Phytologist</i>. Wiley, 2014. <a href=\"https://doi.org/10.1111/nph.12614\">https://doi.org/10.1111/nph.12614</a>.","apa":"Fatichi, S., Leuzinger, S., &#38; Körner, C. (2014). Moving beyond photosynthesis: from carbon source to sink‐driven vegetation modeling. <i>New Phytologist</i>. Wiley. <a href=\"https://doi.org/10.1111/nph.12614\">https://doi.org/10.1111/nph.12614</a>","mla":"Fatichi, Simone, et al. “Moving beyond Photosynthesis: From Carbon Source to Sink‐driven Vegetation Modeling.” <i>New Phytologist</i>, vol. 201, no. 4, Wiley, 2014, pp. 1086–95, doi:<a href=\"https://doi.org/10.1111/nph.12614\">10.1111/nph.12614</a>.","ama":"Fatichi S, Leuzinger S, Körner C. Moving beyond photosynthesis: from carbon source to sink‐driven vegetation modeling. <i>New Phytologist</i>. 2014;201(4):1086-1095. doi:<a href=\"https://doi.org/10.1111/nph.12614\">10.1111/nph.12614</a>","ieee":"S. Fatichi, S. Leuzinger, and C. Körner, “Moving beyond photosynthesis: from carbon source to sink‐driven vegetation modeling,” <i>New Phytologist</i>, vol. 201, no. 4. Wiley, pp. 1086–1095, 2014.","ista":"Fatichi S, Leuzinger S, Körner C. 2014. Moving beyond photosynthesis: from carbon source to sink‐driven vegetation modeling. New Phytologist. 201(4), 1086–1095.","short":"S. Fatichi, S. Leuzinger, C. Körner, New Phytologist 201 (2014) 1086–1095."},"publication_identifier":{"eissn":["1469-8137"],"issn":["0028-646X"]},"publication":"New Phytologist","issue":"4","scopus_import":"1","language":[{"iso":"eng"}],"OA_type":"free access","OA_place":"publisher"}]