[{"author":[{"full_name":"Scheele, Colinda","last_name":"Scheele","first_name":"Colinda"},{"last_name":"Hannezo","orcid":"0000-0001-6005-1561","first_name":"Edouard B","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87","full_name":"Hannezo, Edouard B"},{"last_name":"Muraro","first_name":"Mauro","full_name":"Muraro, Mauro"},{"full_name":"Zomer, Anoek","first_name":"Anoek","last_name":"Zomer"},{"first_name":"Nathalia","last_name":"Langedijk","full_name":"Langedijk, Nathalia"},{"last_name":"Van Oudenaarden","first_name":"Alexander","full_name":"Van Oudenaarden, Alexander"},{"full_name":"Simons, Benjamin","last_name":"Simons","first_name":"Benjamin"},{"full_name":"Van Rheenen, Jacco","first_name":"Jacco","last_name":"Van Rheenen"}],"day":"16","issue":"7641","date_updated":"2021-01-12T08:22:01Z","extern":"1","publisher":"Nature Publishing Group","user_id":"3E5EF7F0-F248-11E8-B48F-1D18A9856A87","abstract":[{"lang":"eng","text":"During puberty, the mouse mammary gland develops into a highly branched epithelial network. Owing to the absence of exclusive stem cell markers, the location, multiplicity, dynamics and fate of mammary stem cells (MaSCs), which drive branching morphogenesis, are unknown. Here we show that morphogenesis is driven by proliferative terminal end buds that terminate or bifurcate with near equal probability, in a stochastic and time-invariant manner, leading to a heterogeneous epithelial network. We show that the majority of terminal end bud cells function as highly proliferative, lineage-committed MaSCs that are heterogeneous in their expression profile and short-term contribution to ductal extension. Yet, through cell rearrangements during terminal end bud bifurcation, each MaSC is able to contribute actively to long-term growth. Our study shows that the behaviour of MaSCs is not directly linked to a single expression profile. Instead, morphogenesis relies upon lineage-restricted heterogeneous MaSC populations that function as single equipotent pools in the long term."}],"month":"02","date_published":"2017-02-16T00:00:00Z","publication_status":"published","volume":542,"publist_id":"6505","_id":"934","date_created":"2018-12-11T11:49:17Z","citation":{"short":"C. Scheele, E.B. Hannezo, M. Muraro, A. Zomer, N. Langedijk, A. Van Oudenaarden, B. Simons, J. Van Rheenen, Nature 542 (2017) 313–317.","ieee":"C. Scheele <i>et al.</i>, “Identity and dynamics of mammary stem cells during branching morphogenesis,” <i>Nature</i>, vol. 542, no. 7641. Nature Publishing Group, pp. 313–317, 2017.","apa":"Scheele, C., Hannezo, E. B., Muraro, M., Zomer, A., Langedijk, N., Van Oudenaarden, A., … Van Rheenen, J. (2017). Identity and dynamics of mammary stem cells during branching morphogenesis. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature21046\">https://doi.org/10.1038/nature21046</a>","ama":"Scheele C, Hannezo EB, Muraro M, et al. Identity and dynamics of mammary stem cells during branching morphogenesis. <i>Nature</i>. 2017;542(7641):313-317. doi:<a href=\"https://doi.org/10.1038/nature21046\">10.1038/nature21046</a>","ista":"Scheele C, Hannezo EB, Muraro M, Zomer A, Langedijk N, Van Oudenaarden A, Simons B, Van Rheenen J. 2017. Identity and dynamics of mammary stem cells during branching morphogenesis. Nature. 542(7641), 313–317.","mla":"Scheele, Colinda, et al. “Identity and Dynamics of Mammary Stem Cells during Branching Morphogenesis.” <i>Nature</i>, vol. 542, no. 7641, Nature Publishing Group, 2017, pp. 313–17, doi:<a href=\"https://doi.org/10.1038/nature21046\">10.1038/nature21046</a>.","chicago":"Scheele, Colinda, Edouard B Hannezo, Mauro Muraro, Anoek Zomer, Nathalia Langedijk, Alexander Van Oudenaarden, Benjamin Simons, and Jacco Van Rheenen. “Identity and Dynamics of Mammary Stem Cells during Branching Morphogenesis.” <i>Nature</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/nature21046\">https://doi.org/10.1038/nature21046</a>."},"language":[{"iso":"eng"}],"quality_controlled":"1","publication_identifier":{"issn":["00280836"]},"intvolume":"       542","publication":"Nature","type":"journal_article","page":"313 - 317","title":"Identity and dynamics of mammary stem cells during branching morphogenesis","status":"public","year":"2017","doi":"10.1038/nature21046","oa_version":"None"},{"author":[{"first_name":"Diana","last_name":"Pinheiro","full_name":"Pinheiro, Diana"},{"full_name":"Hannezo, Edouard B","orcid":"0000-0001-6005-1561","first_name":"Edouard B","last_name":"Hannezo","id":"3A9DB764-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Herszterg, Sophie","last_name":"Herszterg","first_name":"Sophie"},{"first_name":"Floris","last_name":"Bosveld","full_name":"Bosveld, Floris"},{"last_name":"Gaugué","first_name":"Isabelle","full_name":"Gaugué, Isabelle"},{"last_name":"Balakireva","first_name":"Maria","full_name":"Balakireva, Maria"},{"full_name":"Wang, Zhimin","first_name":"Zhimin","last_name":"Wang"},{"full_name":"Cristo, Inês","first_name":"Inês","last_name":"Cristo"},{"last_name":"Rigaud","first_name":"Stéphane","full_name":"Rigaud, Stéphane"},{"first_name":"Olga","last_name":"Markova","full_name":"Markova, Olga"},{"last_name":"Bellaïche","first_name":"Yohanns","full_name":"Bellaïche, Yohanns"}],"issue":"7652","date_updated":"2021-01-12T08:22:02Z","extern":"1","day":"04","publisher":"Nature Publishing Group","user_id":"4435EBFC-F248-11E8-B48F-1D18A9856A87","abstract":[{"text":"During epithelial cytokinesis, the remodelling of adhesive cell-cell contacts between the dividing cell and its neighbours has profound implications for the integrity, arrangement and morphogenesis of proliferative tissues. In both vertebrates and invertebrates, this remodelling requires the activity of non-muscle myosin II (MyoII) in the interphasic cells neighbouring the dividing cell. However, the mechanisms that coordinate cytokinesis and MyoII activity in the neighbours are unknown. Here we show that in the Drosophila notum epithelium, each cell division is associated with a mechanosensing and transmission event that controls MyoII dynamics in neighbouring cells. We find that the ring pulling forces promote local junction elongation, which results in local E-cadherin dilution at the ingressing adherens junction. In turn, the reduction in E-cadherin concentration and the contractility of the neighbouring cells promote self-organized actomyosin flows, ultimately leading to accumulation of MyoII at the base of the ingressing junction. Although force transduction has been extensively studied in the context of adherens junction reinforcement to stabilize adhesive cell-cell contacts, we propose an alternative mechanosensing mechanism that coordinates actomyosin dynamics between epithelial cells and sustains the remodelling of the adherens junction in response to mechanical forces.","lang":"eng"}],"date_published":"2017-05-04T00:00:00Z","month":"05","publist_id":"6504","volume":545,"publication_status":"published","_id":"937","date_created":"2018-12-11T11:49:18Z","language":[{"iso":"eng"}],"citation":{"ista":"Pinheiro D, Hannezo EB, Herszterg S, Bosveld F, Gaugué I, Balakireva M, Wang Z, Cristo I, Rigaud S, Markova O, Bellaïche Y. 2017. Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows. Nature. 545(7652), 103–107.","chicago":"Pinheiro, Diana, Edouard B Hannezo, Sophie Herszterg, Floris Bosveld, Isabelle Gaugué, Maria Balakireva, Zhimin Wang, et al. “Transmission of Cytokinesis Forces via E Cadherin Dilution and Actomyosin Flows.” <i>Nature</i>. Nature Publishing Group, 2017. <a href=\"https://doi.org/10.1038/nature22041\">https://doi.org/10.1038/nature22041</a>.","mla":"Pinheiro, Diana, et al. “Transmission of Cytokinesis Forces via E Cadherin Dilution and Actomyosin Flows.” <i>Nature</i>, vol. 545, no. 7652, Nature Publishing Group, 2017, pp. 103–07, doi:<a href=\"https://doi.org/10.1038/nature22041\">10.1038/nature22041</a>.","short":"D. Pinheiro, E.B. Hannezo, S. Herszterg, F. Bosveld, I. Gaugué, M. Balakireva, Z. Wang, I. Cristo, S. Rigaud, O. Markova, Y. Bellaïche, Nature 545 (2017) 103–107.","apa":"Pinheiro, D., Hannezo, E. B., Herszterg, S., Bosveld, F., Gaugué, I., Balakireva, M., … Bellaïche, Y. (2017). Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows. <i>Nature</i>. Nature Publishing Group. <a href=\"https://doi.org/10.1038/nature22041\">https://doi.org/10.1038/nature22041</a>","ieee":"D. Pinheiro <i>et al.</i>, “Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows,” <i>Nature</i>, vol. 545, no. 7652. Nature Publishing Group, pp. 103–107, 2017.","ama":"Pinheiro D, Hannezo EB, Herszterg S, et al. Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows. <i>Nature</i>. 2017;545(7652):103-107. doi:<a href=\"https://doi.org/10.1038/nature22041\">10.1038/nature22041</a>"},"quality_controlled":"1","publication_identifier":{"issn":["00280836"]},"intvolume":"       545","page":"103 - 107","publication":"Nature","type":"journal_article","title":"Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows","status":"public","year":"2017","doi":"10.1038/nature22041","oa_version":"None"}]