---
_id: '934'
abstract:
- lang: eng
  text: During puberty, the mouse mammary gland develops into a highly branched epithelial
    network. Owing to the absence of exclusive stem cell markers, the location, multiplicity,
    dynamics and fate of mammary stem cells (MaSCs), which drive branching morphogenesis,
    are unknown. Here we show that morphogenesis is driven by proliferative terminal
    end buds that terminate or bifurcate with near equal probability, in a stochastic
    and time-invariant manner, leading to a heterogeneous epithelial network. We show
    that the majority of terminal end bud cells function as highly proliferative,
    lineage-committed MaSCs that are heterogeneous in their expression profile and
    short-term contribution to ductal extension. Yet, through cell rearrangements
    during terminal end bud bifurcation, each MaSC is able to contribute actively
    to long-term growth. Our study shows that the behaviour of MaSCs is not directly
    linked to a single expression profile. Instead, morphogenesis relies upon lineage-restricted
    heterogeneous MaSC populations that function as single equipotent pools in the
    long term.
author:
- first_name: Colinda
  full_name: Scheele, Colinda
  last_name: Scheele
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Mauro
  full_name: Muraro, Mauro
  last_name: Muraro
- first_name: Anoek
  full_name: Zomer, Anoek
  last_name: Zomer
- first_name: Nathalia
  full_name: Langedijk, Nathalia
  last_name: Langedijk
- first_name: Alexander
  full_name: Van Oudenaarden, Alexander
  last_name: Van Oudenaarden
- first_name: Benjamin
  full_name: Simons, Benjamin
  last_name: Simons
- first_name: Jacco
  full_name: Van Rheenen, Jacco
  last_name: Van Rheenen
citation:
  ama: Scheele C, Hannezo EB, Muraro M, et al. Identity and dynamics of mammary stem
    cells during branching morphogenesis. <i>Nature</i>. 2017;542(7641):313-317. doi:<a
    href="https://doi.org/10.1038/nature21046">10.1038/nature21046</a>
  apa: Scheele, C., Hannezo, E. B., Muraro, M., Zomer, A., Langedijk, N., Van Oudenaarden,
    A., … Van Rheenen, J. (2017). Identity and dynamics of mammary stem cells during
    branching morphogenesis. <i>Nature</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/nature21046">https://doi.org/10.1038/nature21046</a>
  chicago: Scheele, Colinda, Edouard B Hannezo, Mauro Muraro, Anoek Zomer, Nathalia
    Langedijk, Alexander Van Oudenaarden, Benjamin Simons, and Jacco Van Rheenen.
    “Identity and Dynamics of Mammary Stem Cells during Branching Morphogenesis.”
    <i>Nature</i>. Nature Publishing Group, 2017. <a href="https://doi.org/10.1038/nature21046">https://doi.org/10.1038/nature21046</a>.
  ieee: C. Scheele <i>et al.</i>, “Identity and dynamics of mammary stem cells during
    branching morphogenesis,” <i>Nature</i>, vol. 542, no. 7641. Nature Publishing
    Group, pp. 313–317, 2017.
  ista: Scheele C, Hannezo EB, Muraro M, Zomer A, Langedijk N, Van Oudenaarden A,
    Simons B, Van Rheenen J. 2017. Identity and dynamics of mammary stem cells during
    branching morphogenesis. Nature. 542(7641), 313–317.
  mla: Scheele, Colinda, et al. “Identity and Dynamics of Mammary Stem Cells during
    Branching Morphogenesis.” <i>Nature</i>, vol. 542, no. 7641, Nature Publishing
    Group, 2017, pp. 313–17, doi:<a href="https://doi.org/10.1038/nature21046">10.1038/nature21046</a>.
  short: C. Scheele, E.B. Hannezo, M. Muraro, A. Zomer, N. Langedijk, A. Van Oudenaarden,
    B. Simons, J. Van Rheenen, Nature 542 (2017) 313–317.
date_created: 2018-12-11T11:49:17Z
date_published: 2017-02-16T00:00:00Z
date_updated: 2021-01-12T08:22:01Z
day: '16'
doi: 10.1038/nature21046
extern: '1'
intvolume: '       542'
issue: '7641'
language:
- iso: eng
month: '02'
oa_version: None
page: 313 - 317
publication: Nature
publication_identifier:
  issn:
  - '00280836'
publication_status: published
publisher: Nature Publishing Group
publist_id: '6505'
quality_controlled: '1'
status: public
title: Identity and dynamics of mammary stem cells during branching morphogenesis
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 542
year: '2017'
...
---
_id: '937'
abstract:
- lang: eng
  text: During epithelial cytokinesis, the remodelling of adhesive cell-cell contacts
    between the dividing cell and its neighbours has profound implications for the
    integrity, arrangement and morphogenesis of proliferative tissues. In both vertebrates
    and invertebrates, this remodelling requires the activity of non-muscle myosin
    II (MyoII) in the interphasic cells neighbouring the dividing cell. However, the
    mechanisms that coordinate cytokinesis and MyoII activity in the neighbours are
    unknown. Here we show that in the Drosophila notum epithelium, each cell division
    is associated with a mechanosensing and transmission event that controls MyoII
    dynamics in neighbouring cells. We find that the ring pulling forces promote local
    junction elongation, which results in local E-cadherin dilution at the ingressing
    adherens junction. In turn, the reduction in E-cadherin concentration and the
    contractility of the neighbouring cells promote self-organized actomyosin flows,
    ultimately leading to accumulation of MyoII at the base of the ingressing junction.
    Although force transduction has been extensively studied in the context of adherens
    junction reinforcement to stabilize adhesive cell-cell contacts, we propose an
    alternative mechanosensing mechanism that coordinates actomyosin dynamics between
    epithelial cells and sustains the remodelling of the adherens junction in response
    to mechanical forces.
author:
- first_name: Diana
  full_name: Pinheiro, Diana
  last_name: Pinheiro
- first_name: Edouard B
  full_name: Hannezo, Edouard B
  id: 3A9DB764-F248-11E8-B48F-1D18A9856A87
  last_name: Hannezo
  orcid: 0000-0001-6005-1561
- first_name: Sophie
  full_name: Herszterg, Sophie
  last_name: Herszterg
- first_name: Floris
  full_name: Bosveld, Floris
  last_name: Bosveld
- first_name: Isabelle
  full_name: Gaugué, Isabelle
  last_name: Gaugué
- first_name: Maria
  full_name: Balakireva, Maria
  last_name: Balakireva
- first_name: Zhimin
  full_name: Wang, Zhimin
  last_name: Wang
- first_name: Inês
  full_name: Cristo, Inês
  last_name: Cristo
- first_name: Stéphane
  full_name: Rigaud, Stéphane
  last_name: Rigaud
- first_name: Olga
  full_name: Markova, Olga
  last_name: Markova
- first_name: Yohanns
  full_name: Bellaïche, Yohanns
  last_name: Bellaïche
citation:
  ama: Pinheiro D, Hannezo EB, Herszterg S, et al. Transmission of cytokinesis forces
    via E cadherin dilution and actomyosin flows. <i>Nature</i>. 2017;545(7652):103-107.
    doi:<a href="https://doi.org/10.1038/nature22041">10.1038/nature22041</a>
  apa: Pinheiro, D., Hannezo, E. B., Herszterg, S., Bosveld, F., Gaugué, I., Balakireva,
    M., … Bellaïche, Y. (2017). Transmission of cytokinesis forces via E cadherin
    dilution and actomyosin flows. <i>Nature</i>. Nature Publishing Group. <a href="https://doi.org/10.1038/nature22041">https://doi.org/10.1038/nature22041</a>
  chicago: Pinheiro, Diana, Edouard B Hannezo, Sophie Herszterg, Floris Bosveld, Isabelle
    Gaugué, Maria Balakireva, Zhimin Wang, et al. “Transmission of Cytokinesis Forces
    via E Cadherin Dilution and Actomyosin Flows.” <i>Nature</i>. Nature Publishing
    Group, 2017. <a href="https://doi.org/10.1038/nature22041">https://doi.org/10.1038/nature22041</a>.
  ieee: D. Pinheiro <i>et al.</i>, “Transmission of cytokinesis forces via E cadherin
    dilution and actomyosin flows,” <i>Nature</i>, vol. 545, no. 7652. Nature Publishing
    Group, pp. 103–107, 2017.
  ista: Pinheiro D, Hannezo EB, Herszterg S, Bosveld F, Gaugué I, Balakireva M, Wang
    Z, Cristo I, Rigaud S, Markova O, Bellaïche Y. 2017. Transmission of cytokinesis
    forces via E cadherin dilution and actomyosin flows. Nature. 545(7652), 103–107.
  mla: Pinheiro, Diana, et al. “Transmission of Cytokinesis Forces via E Cadherin
    Dilution and Actomyosin Flows.” <i>Nature</i>, vol. 545, no. 7652, Nature Publishing
    Group, 2017, pp. 103–07, doi:<a href="https://doi.org/10.1038/nature22041">10.1038/nature22041</a>.
  short: D. Pinheiro, E.B. Hannezo, S. Herszterg, F. Bosveld, I. Gaugué, M. Balakireva,
    Z. Wang, I. Cristo, S. Rigaud, O. Markova, Y. Bellaïche, Nature 545 (2017) 103–107.
date_created: 2018-12-11T11:49:18Z
date_published: 2017-05-04T00:00:00Z
date_updated: 2021-01-12T08:22:02Z
day: '04'
doi: 10.1038/nature22041
extern: '1'
intvolume: '       545'
issue: '7652'
language:
- iso: eng
month: '05'
oa_version: None
page: 103 - 107
publication: Nature
publication_identifier:
  issn:
  - '00280836'
publication_status: published
publisher: Nature Publishing Group
publist_id: '6504'
quality_controlled: '1'
status: public
title: Transmission of cytokinesis forces via E cadherin dilution and actomyosin flows
type: journal_article
user_id: 4435EBFC-F248-11E8-B48F-1D18A9856A87
volume: 545
year: '2017'
...