---
OA_place: publisher
OA_type: hybrid
_id: '15408'
abstract:
- lang: eng
  text: "Background: IgE-mediated degranulation of mast cells (MCs) provides rapid
    protection against environmental hazards, including animal venoms. A fraction
    of tissue-resident MCs intimately associates with blood vessels. These perivascular
    MCs were reported to extend projections into the vessel lumen and to be the first
    MCs to acquire intravenously injected IgE, suggesting that IgE loading of MCs
    depends on their vascular association.\r\nObjective: We sought to elucidate the
    molecular basis of the MC–blood vessel interaction and to determine its relevance
    for IgE-mediated immune responses.\r\nMethods: We selectively inactivated the
    Itgb1 gene, encoding the β1 chain of integrin adhesion molecules (ITGB1), in MCs
    by conditional gene targeting in mice. We analyzed skin MCs for blood vessel association,
    surface IgE density, and capability to bind circulating antibody specific for
    MC surface molecules, as well as in vivo responses to antigen administered via
    different routes.\r\nResults: Lack of ITGB1 expression severely compromised MC–blood
    vessel association. ITGB1-deficient MCs showed normal densities of surface IgE
    but reduced binding of intravenously injected antibodies. While their capacity
    to degranulate in response to IgE ligation in vivo was unimpaired, anaphylactic
    responses to antigen circulating in the vasculature were largely abolished.\r\nConclusions:
    ITGB1-mediated association of MCs with blood vessels is key for MC immune surveillance
    of blood vessel content, but is dispensable for slow steady-state loading of endogenous
    IgE onto tissue-resident MCs."
acknowledgement: "This work was funded by Deutsche Forschungsgemeinschaft, Germany,
  grants RO2133/ 9-1 and RO2133/ 9-2 in the setting of FOR2599 and TR156 project C11
  (Project-ID 246807620–TRR 156) to A. Roers and Springboard-to-Postdoc grant of the
  Dresden International Graduate School for Biomedicine and Bioengineering (DIGS-BB),
  Dresden, Germany, and Fond zur Förderung der Wissenschaftlichen Forschung (FWF),
  Austria, Hertha Firnberg grant (project number T-1219) to A. Polikarpova.\r\nWe
  thank Dr Michael Gerlach, Core Facility Cellular Imaging, Faculty of Medicine Carl
  Gustav Carus, TU Dresden, for expert support of in vivo imaging experiments; Grace
  Wurigamule for help with 2-photon imaging and flow cytometric analysis of mouse
  skin; and Christina Hiller, Livia Schulze, Madelaine Rickauer, and Christa Haase
  for providing expert technical assistance."
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Kristina
  full_name: Link, Kristina
  last_name: Link
- first_name: Lina
  full_name: Muhandes, Lina
  last_name: Muhandes
- first_name: Anastasia
  full_name: Polikarpova, Anastasia
  last_name: Polikarpova
- first_name: Tim
  full_name: Lämmermann, Tim
  last_name: Lämmermann
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Reinhard
  full_name: Fässler, Reinhard
  last_name: Fässler
- first_name: Axel
  full_name: Roers, Axel
  last_name: Roers
citation:
  ama: Link K, Muhandes L, Polikarpova A, et al. Integrin β1–mediated mast cell immune-surveillance
    of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. 2024;154(3):745-753.
    doi:<a href="https://doi.org/10.1016/j.jaci.2024.03.022">10.1016/j.jaci.2024.03.022</a>
  apa: Link, K., Muhandes, L., Polikarpova, A., Lämmermann, T., Sixt, M. K., Fässler,
    R., &#38; Roers, A. (2024). Integrin β1–mediated mast cell immune-surveillance
    of blood vessel content. <i>Journal of Allergy and Clinical Immunology</i>. Elsevier.
    <a href="https://doi.org/10.1016/j.jaci.2024.03.022">https://doi.org/10.1016/j.jaci.2024.03.022</a>
  chicago: Link, Kristina, Lina Muhandes, Anastasia Polikarpova, Tim Lämmermann, Michael
    K Sixt, Reinhard Fässler, and Axel Roers. “Integrin Β1–Mediated Mast Cell Immune-Surveillance
    of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>. Elsevier,
    2024. <a href="https://doi.org/10.1016/j.jaci.2024.03.022">https://doi.org/10.1016/j.jaci.2024.03.022</a>.
  ieee: K. Link <i>et al.</i>, “Integrin β1–mediated mast cell immune-surveillance
    of blood vessel content,” <i>Journal of Allergy and Clinical Immunology</i>, vol.
    154, no. 3. Elsevier, pp. 745–753, 2024.
  ista: Link K, Muhandes L, Polikarpova A, Lämmermann T, Sixt MK, Fässler R, Roers
    A. 2024. Integrin β1–mediated mast cell immune-surveillance of blood vessel content.
    Journal of Allergy and Clinical Immunology. 154(3), 745–753.
  mla: Link, Kristina, et al. “Integrin Β1–Mediated Mast Cell Immune-Surveillance
    of Blood Vessel Content.” <i>Journal of Allergy and Clinical Immunology</i>, vol.
    154, no. 3, Elsevier, 2024, pp. 745–53, doi:<a href="https://doi.org/10.1016/j.jaci.2024.03.022">10.1016/j.jaci.2024.03.022</a>.
  short: K. Link, L. Muhandes, A. Polikarpova, T. Lämmermann, M.K. Sixt, R. Fässler,
    A. Roers, Journal of Allergy and Clinical Immunology 154 (2024) 745–753.
date_created: 2024-05-19T22:01:13Z
date_published: 2024-09-01T00:00:00Z
date_updated: 2025-09-08T07:28:25Z
day: '01'
ddc:
- '570'
department:
- _id: MiSi
doi: 10.1016/j.jaci.2024.03.022
external_id:
  isi:
  - '001308886700001'
  pmid:
  - '38636606'
file:
- access_level: open_access
  checksum: 6a5af05082e1869d7cad6406fa4eb76c
  content_type: application/pdf
  creator: dernst
  date_created: 2025-01-13T10:55:28Z
  date_updated: 2025-01-13T10:55:28Z
  file_id: '18840'
  file_name: 2024_JourAllergyClinicalImm_Link.pdf
  file_size: 1792425
  relation: main_file
  success: 1
file_date_updated: 2025-01-13T10:55:28Z
has_accepted_license: '1'
intvolume: '       154'
isi: 1
issue: '3'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '09'
oa: 1
oa_version: Published Version
page: 745-753
pmid: 1
publication: Journal of Allergy and Clinical Immunology
publication_identifier:
  eissn:
  - 1097-6825
  issn:
  - 0091-6749
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Integrin β1–mediated mast cell immune-surveillance of blood vessel content
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 154
year: '2024'
...
---
_id: '8231'
article_processing_charge: No
article_type: letter_note
author:
- first_name: Judit
  full_name: Fazekas-Singer, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Fazekas-Singer
  orcid: 0000-0002-8777-3502
- first_name: Josef
  full_name: Singer, Josef
  last_name: Singer
- first_name: Kristina M.
  full_name: Ilieva, Kristina M.
  last_name: Ilieva
- first_name: Miroslawa
  full_name: Matz, Miroslawa
  last_name: Matz
- first_name: Ina
  full_name: Herrmann, Ina
  last_name: Herrmann
- first_name: Edzard
  full_name: Spillner, Edzard
  last_name: Spillner
- first_name: Sophia N.
  full_name: Karagiannis, Sophia N.
  last_name: Karagiannis
- first_name: Erika
  full_name: Jensen-Jarolim, Erika
  last_name: Jensen-Jarolim
citation:
  ama: 'Singer J, Singer J, Ilieva KM, et al. AllergoOncology: Generating a canine
    anticancer IgE against the epidermal growth factor receptor. <i>Journal of Allergy
    and Clinical Immunology</i>. 2018;142(3):973-976.e11. doi:<a href="https://doi.org/10.1016/j.jaci.2018.04.021">10.1016/j.jaci.2018.04.021</a>'
  apa: 'Singer, J., Singer, J., Ilieva, K. M., Matz, M., Herrmann, I., Spillner, E.,
    … Jensen-Jarolim, E. (2018). AllergoOncology: Generating a canine anticancer IgE
    against the epidermal growth factor receptor. <i>Journal of Allergy and Clinical
    Immunology</i>. Elsevier. <a href="https://doi.org/10.1016/j.jaci.2018.04.021">https://doi.org/10.1016/j.jaci.2018.04.021</a>'
  chicago: 'Singer, Judit, Josef Singer, Kristina M. Ilieva, Miroslawa Matz, Ina Herrmann,
    Edzard Spillner, Sophia N. Karagiannis, and Erika Jensen-Jarolim. “AllergoOncology:
    Generating a Canine Anticancer IgE against the Epidermal Growth Factor Receptor.”
    <i>Journal of Allergy and Clinical Immunology</i>. Elsevier, 2018. <a href="https://doi.org/10.1016/j.jaci.2018.04.021">https://doi.org/10.1016/j.jaci.2018.04.021</a>.'
  ieee: 'J. Singer <i>et al.</i>, “AllergoOncology: Generating a canine anticancer
    IgE against the epidermal growth factor receptor,” <i>Journal of Allergy and Clinical
    Immunology</i>, vol. 142, no. 3. Elsevier, p. 973–976.e11, 2018.'
  ista: 'Singer J, Singer J, Ilieva KM, Matz M, Herrmann I, Spillner E, Karagiannis
    SN, Jensen-Jarolim E. 2018. AllergoOncology: Generating a canine anticancer IgE
    against the epidermal growth factor receptor. Journal of Allergy and Clinical
    Immunology. 142(3), 973–976.e11.'
  mla: 'Singer, Judit, et al. “AllergoOncology: Generating a Canine Anticancer IgE
    against the Epidermal Growth Factor Receptor.” <i>Journal of Allergy and Clinical
    Immunology</i>, vol. 142, no. 3, Elsevier, 2018, p. 973–976.e11, doi:<a href="https://doi.org/10.1016/j.jaci.2018.04.021">10.1016/j.jaci.2018.04.021</a>.'
  short: J. Singer, J. Singer, K.M. Ilieva, M. Matz, I. Herrmann, E. Spillner, S.N.
    Karagiannis, E. Jensen-Jarolim, Journal of Allergy and Clinical Immunology 142
    (2018) 973–976.e11.
date_created: 2020-08-10T11:51:36Z
date_published: 2018-09-01T00:00:00Z
date_updated: 2021-01-12T08:17:37Z
day: '01'
doi: 10.1016/j.jaci.2018.04.021
extern: '1'
intvolume: '       142'
issue: '3'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://doi.org/10.1016/j.jaci.2018.04.021
month: '09'
oa: 1
oa_version: Published Version
page: 973-976.e11
publication: Journal of Allergy and Clinical Immunology
publication_identifier:
  issn:
  - 0091-6749
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: 'AllergoOncology: Generating a canine anticancer IgE against the epidermal
  growth factor receptor'
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 142
year: '2018'
...
---
_id: '8242'
article_number: AB101
article_processing_charge: No
article_type: original
author:
- first_name: Lukas
  full_name: Einhorn, Lukas
  last_name: Einhorn
- first_name: Judit
  full_name: Fazekas, Judit
  id: 36432834-F248-11E8-B48F-1D18A9856A87
  last_name: Fazekas
  orcid: 0000-0002-8777-3502
- first_name: Martina
  full_name: Muhr, Martina
  last_name: Muhr
- first_name: Alexandra
  full_name: Schoos, Alexandra
  last_name: Schoos
- first_name: Kumiko
  full_name: Oida, Kumiko
  last_name: Oida
- first_name: Josef
  full_name: Singer, Josef
  last_name: Singer
- first_name: Lucia
  full_name: Panakova, Lucia
  last_name: Panakova
- first_name: Krisztina
  full_name: Manzano-Szalai, Krisztina
  last_name: Manzano-Szalai
- first_name: Erika
  full_name: Jensen-Jarolim, Erika
  last_name: Jensen-Jarolim
citation:
  ama: Einhorn L, Singer J, Muhr M, et al. Generation of recombinant FcεRIα of dog,
    cat and horse for component-resolved allergy diagnosis in veterinary patients.
    <i>Journal of Allergy and Clinical Immunology</i>. 2015;135(2). doi:<a href="https://doi.org/10.1016/j.jaci.2014.12.1263">10.1016/j.jaci.2014.12.1263</a>
  apa: Einhorn, L., Singer, J., Muhr, M., Schoos, A., Oida, K., Singer, J., … Jensen-Jarolim,
    E. (2015). Generation of recombinant FcεRIα of dog, cat and horse for component-resolved
    allergy diagnosis in veterinary patients. <i>Journal of Allergy and Clinical Immunology</i>.
    Elsevier. <a href="https://doi.org/10.1016/j.jaci.2014.12.1263">https://doi.org/10.1016/j.jaci.2014.12.1263</a>
  chicago: Einhorn, Lukas, Judit Singer, Martina Muhr, Alexandra Schoos, Kumiko Oida,
    Josef Singer, Lucia Panakova, Krisztina Manzano-Szalai, and Erika Jensen-Jarolim.
    “Generation of Recombinant FcεRIα of Dog, Cat and Horse for Component-Resolved
    Allergy Diagnosis in Veterinary Patients.” <i>Journal of Allergy and Clinical
    Immunology</i>. Elsevier, 2015. <a href="https://doi.org/10.1016/j.jaci.2014.12.1263">https://doi.org/10.1016/j.jaci.2014.12.1263</a>.
  ieee: L. Einhorn <i>et al.</i>, “Generation of recombinant FcεRIα of dog, cat and
    horse for component-resolved allergy diagnosis in veterinary patients,” <i>Journal
    of Allergy and Clinical Immunology</i>, vol. 135, no. 2. Elsevier, 2015.
  ista: Einhorn L, Singer J, Muhr M, Schoos A, Oida K, Singer J, Panakova L, Manzano-Szalai
    K, Jensen-Jarolim E. 2015. Generation of recombinant FcεRIα of dog, cat and horse
    for component-resolved allergy diagnosis in veterinary patients. Journal of Allergy
    and Clinical Immunology. 135(2), AB101.
  mla: Einhorn, Lukas, et al. “Generation of Recombinant FcεRIα of Dog, Cat and Horse
    for Component-Resolved Allergy Diagnosis in Veterinary Patients.” <i>Journal of
    Allergy and Clinical Immunology</i>, vol. 135, no. 2, AB101, Elsevier, 2015, doi:<a
    href="https://doi.org/10.1016/j.jaci.2014.12.1263">10.1016/j.jaci.2014.12.1263</a>.
  short: L. Einhorn, J. Singer, M. Muhr, A. Schoos, K. Oida, J. Singer, L. Panakova,
    K. Manzano-Szalai, E. Jensen-Jarolim, Journal of Allergy and Clinical Immunology
    135 (2015).
date_created: 2020-08-10T11:54:09Z
date_published: 2015-02-01T00:00:00Z
date_updated: 2021-01-12T08:17:42Z
day: '01'
doi: 10.1016/j.jaci.2014.12.1263
extern: '1'
intvolume: '       135'
issue: '2'
language:
- iso: eng
month: '02'
oa_version: None
publication: Journal of Allergy and Clinical Immunology
publication_identifier:
  issn:
  - 0091-6749
publication_status: published
publisher: Elsevier
quality_controlled: '1'
status: public
title: Generation of recombinant FcεRIα of dog, cat and horse for component-resolved
  allergy diagnosis in veterinary patients
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 135
year: '2015'
...