@article{600,
  abstract     = {Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct “bendability” and “meltability” of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.},
  author       = {Engel, Christoph and Gubbey, Tobias and Neyer, Simon and Sainsbury, Sarah and Oberthuer, Christiane and Baejen, Carlo and Bernecky, Carrie A and Cramer, Patrick},
  issn         = {00928674},
  journal      = {Cell},
  number       = {1},
  pages        = {120 -- 131.e22},
  publisher    = {Cell Press},
  title        = {{Structural basis of RNA polymerase I transcription initiation}},
  doi          = {10.1016/j.cell.2017.03.003},
  volume       = {169},
  year         = {2017},
}