[{"publication_identifier":{"issn":["00928674"]},"publication_status":"published","title":"Structural basis of RNA polymerase I transcription initiation","date_updated":"2025-09-11T07:38:07Z","date_published":"2017-03-23T00:00:00Z","isi":1,"external_id":{"isi":["000397090000013"]},"abstract":[{"lang":"eng","text":"Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct “bendability” and “meltability” of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase."}],"quality_controlled":"1","type":"journal_article","citation":{"ama":"Engel C, Gubbey T, Neyer S, et al. Structural basis of RNA polymerase I transcription initiation. <i>Cell</i>. 2017;169(1):120-131.e22. doi:<a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">10.1016/j.cell.2017.03.003</a>","ieee":"C. Engel <i>et al.</i>, “Structural basis of RNA polymerase I transcription initiation,” <i>Cell</i>, vol. 169, no. 1. Cell Press, p. 120–131.e22, 2017.","mla":"Engel, Christoph, et al. “Structural Basis of RNA Polymerase I Transcription Initiation.” <i>Cell</i>, vol. 169, no. 1, Cell Press, 2017, p. 120–131.e22, doi:<a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">10.1016/j.cell.2017.03.003</a>.","ista":"Engel C, Gubbey T, Neyer S, Sainsbury S, Oberthuer C, Baejen C, Bernecky C, Cramer P. 2017. Structural basis of RNA polymerase I transcription initiation. Cell. 169(1), 120–131.e22.","chicago":"Engel, Christoph, Tobias Gubbey, Simon Neyer, Sarah Sainsbury, Christiane Oberthuer, Carlo Baejen, Carrie Bernecky, and Patrick Cramer. “Structural Basis of RNA Polymerase I Transcription Initiation.” <i>Cell</i>. Cell Press, 2017. <a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">https://doi.org/10.1016/j.cell.2017.03.003</a>.","apa":"Engel, C., Gubbey, T., Neyer, S., Sainsbury, S., Oberthuer, C., Baejen, C., … Cramer, P. (2017). Structural basis of RNA polymerase I transcription initiation. <i>Cell</i>. Cell Press. <a href=\"https://doi.org/10.1016/j.cell.2017.03.003\">https://doi.org/10.1016/j.cell.2017.03.003</a>","short":"C. Engel, T. Gubbey, S. Neyer, S. Sainsbury, C. Oberthuer, C. Baejen, C. Bernecky, P. Cramer, Cell 169 (2017) 120–131.e22."},"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","publication":"Cell","month":"03","_id":"600","status":"public","oa_version":"None","doi":"10.1016/j.cell.2017.03.003","extern":"1","intvolume":"       169","author":[{"full_name":"Engel, Christoph","last_name":"Engel","first_name":"Christoph"},{"first_name":"Tobias","full_name":"Gubbey, Tobias","last_name":"Gubbey"},{"full_name":"Neyer, Simon","last_name":"Neyer","first_name":"Simon"},{"full_name":"Sainsbury, Sarah","last_name":"Sainsbury","first_name":"Sarah"},{"full_name":"Oberthuer, Christiane","last_name":"Oberthuer","first_name":"Christiane"},{"first_name":"Carlo","last_name":"Baejen","full_name":"Baejen, Carlo"},{"full_name":"Bernecky, Carrie A","last_name":"Bernecky","orcid":"0000-0003-0893-7036","id":"2CB9DFE2-F248-11E8-B48F-1D18A9856A87","first_name":"Carrie A"},{"first_name":"Patrick","last_name":"Cramer","full_name":"Cramer, Patrick"}],"volume":169,"date_created":"2018-12-11T11:47:25Z","publisher":"Cell Press","issue":"1","day":"23","publist_id":"7204","article_processing_charge":"No","year":"2017","language":[{"iso":"eng"}],"page":"120 - 131.e22"}]