---
OA_type: closed access
_id: '2651'
abstract:
- lang: eng
  text: The GABAergic system, a major inhibitory regulator in the central nervous
    system, may also play important roles in peripheral nonneuronal tissues and cells.
    Recent studies showed that GABAB receptor is expressed in testis and sperm. To
    understand the role of the GABAergic system in spermiogenesis, we examined cellular
    localization of GABA and GABAB receptor subunits in rat spermatids by immunocytochemistry.
    Immunoreactivity for GABA was detected around acrosomal granules of spermatids
    during the Golgi and cap phases. GABAB(1) immunoreactivity was observed in the
    acrosomal vesicle of spermatids in Golgi phase, and during cap phase, this reactivity
    expanded to the entire region of the acrosome covering the nuclear membrane. The
    level of reactivity decreased gradually with maturation of spermatids. In contrast,
    GABAB(2) immunoreactivity was not observed in spermatids during Golgi phase but
    was detected in the equatorial region during cap phase. Both GABA immunoreactivity
    and GABAB(2) immunoreactivity were transferred to the residual cytoplasm during
    the release of spermatozoa. Electron microscopic immunocytochemistry revealed
    that, during cap phase, GABA and GABAB(1) were distributed within the whole acrosomal
    vesicle but not in the acrosomal granule. GABAB(2) immunoreactivity was observed
    in the narrow space between the inner acrosomal and nuclear membrane and was limited
    to the equatorial region of the spermatid head. These results indicate that the
    GABAergic system might be involved in regulation of spermiogenesis.
article_processing_charge: No
article_type: original
author:
- first_name: Kiyoto
  full_name: Kanbara, Kiyoto
  last_name: Kanbara
- first_name: Keiko
  full_name: Okamoto, Keiko
  last_name: Okamoto
- first_name: Sakashi
  full_name: Nomura, Sakashi
  last_name: Nomura
- first_name: Takeshi
  full_name: Kaneko, Takeshi
  last_name: Kaneko
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Haruhito
  full_name: Azuma, Haruhito
  last_name: Azuma
- first_name: Yoji
  full_name: Katsuoka, Yoji
  last_name: Katsuoka
- first_name: Masahiko
  full_name: Watanabe, Masahiko
  last_name: Watanabe
citation:
  ama: Kanbara K, Okamoto K, Nomura S, et al. Cellular localization of GABA and GABAB
    receptor subunit proteins during spermiogenesis in rat testis. <i>Journal of Andrology</i>.
    2005;26(4):485-493. doi:<a href="https://doi.org/10.2164/jandrol.04185">10.2164/jandrol.04185</a>
  apa: Kanbara, K., Okamoto, K., Nomura, S., Kaneko, T., Shigemoto, R., Azuma, H.,
    … Watanabe, M. (2005). Cellular localization of GABA and GABAB receptor subunit
    proteins during spermiogenesis in rat testis. <i>Journal of Andrology</i>. American
    Society of Andrology. <a href="https://doi.org/10.2164/jandrol.04185">https://doi.org/10.2164/jandrol.04185</a>
  chicago: Kanbara, Kiyoto, Keiko Okamoto, Sakashi Nomura, Takeshi Kaneko, Ryuichi
    Shigemoto, Haruhito Azuma, Yoji Katsuoka, and Masahiko Watanabe. “Cellular Localization
    of GABA and GABAB Receptor Subunit Proteins during Spermiogenesis in Rat Testis.”
    <i>Journal of Andrology</i>. American Society of Andrology, 2005. <a href="https://doi.org/10.2164/jandrol.04185">https://doi.org/10.2164/jandrol.04185</a>.
  ieee: K. Kanbara <i>et al.</i>, “Cellular localization of GABA and GABAB receptor
    subunit proteins during spermiogenesis in rat testis,” <i>Journal of Andrology</i>,
    vol. 26, no. 4. American Society of Andrology, pp. 485–493, 2005.
  ista: Kanbara K, Okamoto K, Nomura S, Kaneko T, Shigemoto R, Azuma H, Katsuoka Y,
    Watanabe M. 2005. Cellular localization of GABA and GABAB receptor subunit proteins
    during spermiogenesis in rat testis. Journal of Andrology. 26(4), 485–493.
  mla: Kanbara, Kiyoto, et al. “Cellular Localization of GABA and GABAB Receptor Subunit
    Proteins during Spermiogenesis in Rat Testis.” <i>Journal of Andrology</i>, vol.
    26, no. 4, American Society of Andrology, 2005, pp. 485–93, doi:<a href="https://doi.org/10.2164/jandrol.04185">10.2164/jandrol.04185</a>.
  short: K. Kanbara, K. Okamoto, S. Nomura, T. Kaneko, R. Shigemoto, H. Azuma, Y.
    Katsuoka, M. Watanabe, Journal of Andrology 26 (2005) 485–493.
date_created: 2018-12-11T11:58:52Z
date_published: 2005-07-01T00:00:00Z
date_updated: 2026-08-06T13:42:30Z
day: '01'
doi: 10.2164/jandrol.04185
extern: '1'
external_id:
  pmid:
  - '15955887 '
fulldoi: https://doi.org/10.2164/jandrol.04185
intvolume: '        26'
issue: '4'
language:
- iso: eng
month: '07'
oa_version: None
page: 485 - 493
pmid: 1
publication: Journal of Andrology
publication_identifier:
  issn:
  - '01963635'
publication_status: published
publisher: American Society of Andrology
publist_id: '4246'
status: public
title: Cellular localization of GABA and GABAB receptor subunit proteins during spermiogenesis
  in rat testis
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 26
year: '2005'
...