[{"OA_type":"gold","article_type":"original","month":"10","oa_version":"Published Version","publication_status":"published","publisher":"Embo Press","type":"journal_article","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"OA_place":"publisher","isi":1,"pmid":1,"_id":"18073","department":[{"_id":"MaLo"},{"_id":"JiFr"}],"tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"title":"Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering","date_updated":"2025-09-08T09:22:11Z","acknowledgement":"We thank late Thomas Peterbauer at the Max Perutz Labs Biooptics Light Microscopy Facility for his help and support. We are grateful to Kitti Csalyi and Thomas Sauer at Max Perutz Labs Biooptics FACS facility for their help. We thank Grzegorz Scibisz and Sertan Atilla for their support with the expression and purification of mCherry-IRE1α LD-10His. We are grateful to Aleksandra S Anisimova with her help in the generation of stable cell lines and the statistical analyses of the data. We thank Venja Vieweger for her help with the characterization of the WLLI and D123P IRE1 mutants in cells. We are thankful to Monika Kubickova for the help with the AUC experiments. We acknowledge CF BIC of CIISB, Instruct-CZ Centre, supported by MEYS CR (LM2023042)) and European Regional Development Fund-Project, UP CIISB“ (No. CZ.02.1.01/0.0/0.0/18_046/0015974). We thank the members of the Karagöz lab for the critical reading and editing of the manuscript. We are thankful to our colleagues Diego Acosta-Alvear, Vladislav Belyy, Jirka Peschek, Yasin Dagdas, Javier Martinez, Sascha Martens and Alwin Köhler for their invaluable input on the manuscript. We are grateful to Life Science Editors, especially Katrina Woolcock for her useful edits and comments on the manuscript. We acknowledge funding from Austrian Science Fund (FWF-SFB F79 and FWF-W 1261) to GEK. PK acknowledges the support of the Max Perutz PhD fellowship. GAV is funded by Stand-Alone grants (P30231-B, P30415-B, P36572), Special Research Grant (SFB grant F79), and Doctoral School grant (DK grant W1261) from the Austrian Science Fund (FWF). ES and RC acknowledge support and funding by the Frankfurt Institute of Advanced Studies, the LOEWE Center for Multiscale Modelling in Life Sciences of the state of Hesse, the Collaborative Research Center 1507 “Membrane-associated Protein Assemblies, Machineries, and Supercomplexes” (Project ID 450648163), and the International Max Planck Research School on Cellular Biophysics (to RC), the Center for Scientific Computing of the Goethe University and the Jülich Supercomputing Centre for computational resources and support.","status":"public","volume":43,"external_id":{"isi":["001306286100002"],"pmid":["39232130"]},"author":[{"first_name":"Paulina","full_name":"Kettel, Paulina","last_name":"Kettel"},{"first_name":"Laura","last_name":"Marosits","full_name":"Marosits, Laura"},{"first_name":"Elena","last_name":"Spinetti","full_name":"Spinetti, Elena"},{"first_name":"Michael","last_name":"Rechberger","full_name":"Rechberger, Michael"},{"first_name":"Caterina","last_name":"Giannini","id":"e3fdddd5-f6e0-11ea-865d-ca99ee6367f4","full_name":"Giannini, Caterina"},{"last_name":"Radler","id":"40136C2A-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9198-2182 ","full_name":"Radler, Philipp","first_name":"Philipp"},{"last_name":"Niedermoser","full_name":"Niedermoser, Isabell","first_name":"Isabell"},{"last_name":"Fischer","full_name":"Fischer, Irmgard","first_name":"Irmgard"},{"first_name":"Gijs A.","full_name":"Versteeg, Gijs A.","last_name":"Versteeg"},{"first_name":"Martin","id":"462D4284-F248-11E8-B48F-1D18A9856A87","last_name":"Loose","orcid":"0000-0001-7309-9724","full_name":"Loose, Martin"},{"first_name":"Roberto","last_name":"Covino","full_name":"Covino, Roberto"},{"full_name":"Karagöz, G. Elif","last_name":"Karagöz","first_name":"G. Elif"}],"file":[{"checksum":"04f4df1a561083f2846676442fc4eb3c","content_type":"application/pdf","access_level":"open_access","date_created":"2025-01-13T08:43:20Z","success":1,"file_name":"2024_Embo_Kettel.pdf","file_size":10080854,"creator":"dernst","file_id":"18827","relation":"main_file","date_updated":"2025-01-13T08:43:20Z"}],"date_published":"2024-10-15T00:00:00Z","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","issue":"20","language":[{"iso":"eng"}],"publication":"EMBO Journal","page":"4668-4698","year":"2024","day":"15","date_created":"2024-09-15T22:01:42Z","quality_controlled":"1","citation":{"ieee":"P. Kettel <i>et al.</i>, “Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering,” <i>EMBO Journal</i>, vol. 43, no. 20. Embo Press, pp. 4668–4698, 2024.","short":"P. Kettel, L. Marosits, E. Spinetti, M. Rechberger, C. Giannini, P. Radler, I. Niedermoser, I. Fischer, G.A. Versteeg, M. Loose, R. Covino, G.E. Karagöz, EMBO Journal 43 (2024) 4668–4698.","ama":"Kettel P, Marosits L, Spinetti E, et al. Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering. <i>EMBO Journal</i>. 2024;43(20):4668-4698. doi:<a href=\"https://doi.org/10.1038/s44318-024-00207-0\">10.1038/s44318-024-00207-0</a>","apa":"Kettel, P., Marosits, L., Spinetti, E., Rechberger, M., Giannini, C., Radler, P., … Karagöz, G. E. (2024). Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.1038/s44318-024-00207-0\">https://doi.org/10.1038/s44318-024-00207-0</a>","chicago":"Kettel, Paulina, Laura Marosits, Elena Spinetti, Michael Rechberger, Caterina Giannini, Philipp Radler, Isabell Niedermoser, et al. “Disordered Regions in the IRE1α ER Lumenal Domain Mediate Its Stress-Induced Clustering.” <i>EMBO Journal</i>. Embo Press, 2024. <a href=\"https://doi.org/10.1038/s44318-024-00207-0\">https://doi.org/10.1038/s44318-024-00207-0</a>.","mla":"Kettel, Paulina, et al. “Disordered Regions in the IRE1α ER Lumenal Domain Mediate Its Stress-Induced Clustering.” <i>EMBO Journal</i>, vol. 43, no. 20, Embo Press, 2024, pp. 4668–98, doi:<a href=\"https://doi.org/10.1038/s44318-024-00207-0\">10.1038/s44318-024-00207-0</a>.","ista":"Kettel P, Marosits L, Spinetti E, Rechberger M, Giannini C, Radler P, Niedermoser I, Fischer I, Versteeg GA, Loose M, Covino R, Karagöz GE. 2024. Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced clustering. EMBO Journal. 43(20), 4668–4698."},"scopus_import":"1","doi":"10.1038/s44318-024-00207-0","intvolume":"        43","oa":1,"file_date_updated":"2025-01-13T08:43:20Z","abstract":[{"lang":"eng","text":"Conserved signaling cascades monitor protein-folding homeostasis to ensure proper cellular function. One of the evolutionary conserved key players is IRE1, which maintains endoplasmic reticulum (ER) homeostasis through the unfolded protein response (UPR). Upon accumulation of misfolded proteins in the ER, IRE1 forms clusters on the ER membrane to initiate UPR signaling. What regulates IRE1 cluster formation is not fully understood. Here, we show that the ER lumenal domain (LD) of human IRE1α forms biomolecular condensates in vitro. IRE1α LD condensates were stabilized both by binding to unfolded polypeptides as well as by tethering to model membranes, suggesting their role in assembling IRE1α into signaling-competent stable clusters. Molecular dynamics simulations indicated that weak multivalent interactions drive IRE1α LD clustering. Mutagenesis experiments identified disordered regions in IRE1α LD to control its clustering in vitro and in cells. Importantly, dysregulated clustering of IRE1α mutants led to defects in IRE1α signaling. Our results revealed that disordered regions in IRE1α LD control its clustering and suggest their role as a common strategy in regulating protein assembly on membranes."}],"ddc":["570"],"has_accepted_license":"1","article_processing_charge":"Yes"},{"acknowledgement":"We thank Christoph Mayr and Bingzhi Wang for initial experiments on amoeboid nucleokinesis, Ana-Maria Lennon-Duménil and Aline Yatim for bone marrow from MyoIIA-Flox*CD11c-Cre mice, Michael Sixt and Aglaja Kopf for EMTB-mCherry, EB3-mCherry, Lifeact-GFP, Lfc knockout, and Myh9-GFP expressing HoxB8 cells, Malte Benjamin Braun, Mauricio Ruiz, and Madeleine T. Schmitt for critical reading of the manuscript, and the Core Facility Bioimaging, the Core Facility Flow Cytometry, and the Animal Core Facility of the Biomedical Center (BMC) for excellent support. This study was supported by the Peter Hans Hofschneider Professorship of the foundation “Stiftung Experimentelle Biomedizin” (to JR), the LMU Institutional Strategy LMU-Excellent within the framework of the German Excellence Initiative (to JR), and the Deutsche Forschungsgemeinschaft (DFG; German Research Foundation; SFB914 project A12, to JR), and the CZI grant DAF2020-225401 (https://doi.org/10.37921/120055ratwvi) from the Chan Zuckerberg Initiative DAF (to RH; an advised fund of Silicon Valley Community Foundation (funder https://doi.org/10.13039/100014989)). Open Access funding enabled and organized by Projekt DEAL.","status":"public","date_published":"2023-11-21T00:00:00Z","file":[{"date_updated":"2023-11-27T08:45:56Z","relation":"main_file","file_id":"14611","creator":"dernst","file_size":4862497,"success":1,"file_name":"2023_EmboJournal_Kroll.pdf","date_created":"2023-11-27T08:45:56Z","content_type":"application/pdf","access_level":"open_access","checksum":"6261d0041c7e8d284c39712c40079730"}],"user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","author":[{"last_name":"Kroll","full_name":"Kroll, Janina","first_name":"Janina"},{"last_name":"Hauschild","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","full_name":"Hauschild, Robert","orcid":"0000-0001-9843-3522","first_name":"Robert"},{"full_name":"Kuznetcov, Arthur","last_name":"Kuznetcov","first_name":"Arthur"},{"last_name":"Stefanowski","full_name":"Stefanowski, Kasia","first_name":"Kasia"},{"first_name":"Monika D.","full_name":"Hermann, Monika D.","last_name":"Hermann"},{"first_name":"Jack","orcid":"0000-0001-5145-4609","full_name":"Merrin, Jack","last_name":"Merrin","id":"4515C308-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Lubuna B","full_name":"Shafeek, Lubuna B","orcid":"0000-0001-7180-6050","id":"3CD37A82-F248-11E8-B48F-1D18A9856A87","last_name":"Shafeek"},{"first_name":"Annette","full_name":"Müller-Taubenberger, Annette","last_name":"Müller-Taubenberger"},{"first_name":"Jörg","last_name":"Renkawitz","id":"3F0587C8-F248-11E8-B48F-1D18A9856A87","full_name":"Renkawitz, Jörg","orcid":"0000-0003-2856-3369"}],"external_id":{"isi":["001120971800001"],"pmid":["37987147"]},"publication":"EMBO Journal","language":[{"iso":"eng"}],"year":"2023","citation":{"ama":"Kroll J, Hauschild R, Kuznetcov A, et al. Adaptive pathfinding by nucleokinesis during amoeboid migration. <i>EMBO Journal</i>. 2023. doi:<a href=\"https://doi.org/10.15252/embj.2023114557\">10.15252/embj.2023114557</a>","short":"J. Kroll, R. Hauschild, A. Kuznetcov, K. Stefanowski, M.D. Hermann, J. Merrin, L.B. Shafeek, A. Müller-Taubenberger, J. Renkawitz, EMBO Journal (2023).","ieee":"J. Kroll <i>et al.</i>, “Adaptive pathfinding by nucleokinesis during amoeboid migration,” <i>EMBO Journal</i>. Embo Press, 2023.","chicago":"Kroll, Janina, Robert Hauschild, Arthur Kuznetcov, Kasia Stefanowski, Monika D. Hermann, Jack Merrin, Lubuna B Shafeek, Annette Müller-Taubenberger, and Jörg Renkawitz. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.” <i>EMBO Journal</i>. Embo Press, 2023. <a href=\"https://doi.org/10.15252/embj.2023114557\">https://doi.org/10.15252/embj.2023114557</a>.","mla":"Kroll, Janina, et al. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.” <i>EMBO Journal</i>, e114557, Embo Press, 2023, doi:<a href=\"https://doi.org/10.15252/embj.2023114557\">10.15252/embj.2023114557</a>.","apa":"Kroll, J., Hauschild, R., Kuznetcov, A., Stefanowski, K., Hermann, M. D., Merrin, J., … Renkawitz, J. (2023). Adaptive pathfinding by nucleokinesis during amoeboid migration. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2023114557\">https://doi.org/10.15252/embj.2023114557</a>","ista":"Kroll J, Hauschild R, Kuznetcov A, Stefanowski K, Hermann MD, Merrin J, Shafeek LB, Müller-Taubenberger A, Renkawitz J. 2023. Adaptive pathfinding by nucleokinesis during amoeboid migration. EMBO Journal., e114557."},"quality_controlled":"1","date_created":"2023-08-01T08:59:06Z","scopus_import":"1","day":"21","doi":"10.15252/embj.2023114557","abstract":[{"text":"Motile cells moving in multicellular organisms encounter microenvironments of locally heterogeneous mechanochemical composition. Individual compositional parameters like chemotactic signals, adhesiveness, and pore sizes are well known to be sensed by motile cells, providing individual guidance cues for cellular pathfinding. However, motile cells encounter diverse mechanochemical signals at the same time, raising the question of how cells respond to locally diverse and potentially competing signals on their migration routes. Here, we reveal that motile amoeboid cells require nuclear repositioning, termed nucleokinesis, for adaptive pathfinding in heterogeneous mechanochemical microenvironments. Using mammalian immune cells and the amoeba<jats:italic>Dictyostelium discoideum</jats:italic>, we discover that frequent, rapid and long-distance nucleokinesis is a basic component of amoeboid pathfinding, enabling cells to reorientate quickly between locally competing cues. Amoeboid nucleokinesis comprises a two-step cell polarity switch and is driven by myosin II-forces, sliding the nucleus from a ‘losing’ to the ‘winning’ leading edge to re-adjust the nuclear to the cellular path. Impaired nucleokinesis distorts fast path adaptions and causes cellular arrest in the microenvironment. Our findings establish that nucleokinesis is required for amoeboid cell navigation. Given that motile single-cell amoebae, many immune cells, and some cancer cells utilize an amoeboid migration strategy, these results suggest that amoeboid nucleokinesis underlies cellular navigation during unicellular biology, immunity, and disease.","lang":"eng"}],"ddc":["570"],"oa":1,"file_date_updated":"2023-11-27T08:45:56Z","article_number":"e114557","article_processing_charge":"Yes (via OA deal)","has_accepted_license":"1","month":"11","article_type":"original","publisher":"Embo Press","type":"journal_article","publication_status":"published","oa_version":"Published Version","isi":1,"publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"_id":"13342","pmid":1,"tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"title":"Adaptive pathfinding by nucleokinesis during amoeboid migration","department":[{"_id":"NanoFab"},{"_id":"Bio"}],"date_updated":"2025-09-09T12:44:04Z"},{"language":[{"iso":"eng"}],"publication":"The EMBO Journal","issue":"21","year":"2021","status":"public","date_published":"2021-11-02T00:00:00Z","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","external_id":{"pmid":["34524703"]},"author":[{"last_name":"Geiger","full_name":"Geiger, Florian","first_name":"Florian"},{"first_name":"Julia","last_name":"Acker","full_name":"Acker, Julia"},{"full_name":"Papa, Guido","last_name":"Papa","first_name":"Guido"},{"first_name":"Xinyu","last_name":"Wang","full_name":"Wang, Xinyu"},{"full_name":"Arter, William E","last_name":"Arter","first_name":"William E"},{"last_name":"Saar","full_name":"Saar, Kadi L","first_name":"Kadi L"},{"full_name":"Erkamp, Nadia A","last_name":"Erkamp","first_name":"Nadia A"},{"full_name":"Qi, Runzhang","last_name":"Qi","first_name":"Runzhang"},{"first_name":"Jack Peter Kelly","last_name":"Bravo","id":"96aecfa5-8931-11ee-af30-aa6a5d6eee0e","full_name":"Bravo, Jack Peter Kelly","orcid":"0000-0003-0456-0753"},{"first_name":"Sebastian","last_name":"Strauss","full_name":"Strauss, Sebastian"},{"last_name":"Krainer","full_name":"Krainer, Georg","first_name":"Georg"},{"full_name":"Burrone, Oscar R","last_name":"Burrone","first_name":"Oscar R"},{"last_name":"Jungmann","full_name":"Jungmann, Ralf","first_name":"Ralf"},{"last_name":"Knowles","full_name":"Knowles, Tuomas PJ","first_name":"Tuomas PJ"},{"last_name":"Engelke","full_name":"Engelke, Hanna","first_name":"Hanna"},{"last_name":"Borodavka","full_name":"Borodavka, Alexander","first_name":"Alexander"}],"volume":40,"extern":"1","abstract":[{"lang":"eng","text":"RNA viruses induce the formation of subcellular organelles that provide microenvironments conducive to their replication. Here we show that replication factories of rotaviruses represent protein‐RNA condensates that are formed via liquid–liquid phase separation of the viroplasm‐forming proteins NSP5 and rotavirus RNA chaperone NSP2. Upon mixing, these proteins readily form condensates at physiologically relevant low micromolar concentrations achieved in the cytoplasm of virus‐infected cells. Early infection stage condensates could be reversibly dissolved by 1,6‐hexanediol, as well as propylene glycol that released rotavirus transcripts from these condensates. During the early stages of infection, propylene glycol treatments reduced viral replication and phosphorylation of the condensate‐forming protein NSP5. During late infection, these condensates exhibited altered material properties and became resistant to propylene glycol, coinciding with hyperphosphorylation of NSP5. Some aspects of the assembly of cytoplasmic rotavirus replication factories mirror the formation of other ribonucleoprotein granules. Such viral RNA‐rich condensates that support replication of multi‐segmented genomes represent an attractive target for developing novel therapeutic approaches."}],"oa":1,"article_processing_charge":"Yes","article_number":"e107711","scopus_import":"1","date_created":"2024-03-20T10:42:39Z","citation":{"ista":"Geiger F, Acker J, Papa G, Wang X, Arter WE, Saar KL, Erkamp NA, Qi R, Bravo JPK, Strauss S, Krainer G, Burrone OR, Jungmann R, Knowles TP, Engelke H, Borodavka A. 2021. Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses. The EMBO Journal. 40(21), e107711.","apa":"Geiger, F., Acker, J., Papa, G., Wang, X., Arter, W. E., Saar, K. L., … Borodavka, A. (2021). Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses. <i>The EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2021107711\">https://doi.org/10.15252/embj.2021107711</a>","chicago":"Geiger, Florian, Julia Acker, Guido Papa, Xinyu Wang, William E Arter, Kadi L Saar, Nadia A Erkamp, et al. “Liquid–Liquid Phase Separation Underpins the Formation of Replication Factories in Rotaviruses.” <i>The EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2021107711\">https://doi.org/10.15252/embj.2021107711</a>.","mla":"Geiger, Florian, et al. “Liquid–Liquid Phase Separation Underpins the Formation of Replication Factories in Rotaviruses.” <i>The EMBO Journal</i>, vol. 40, no. 21, e107711, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2021107711\">10.15252/embj.2021107711</a>.","ama":"Geiger F, Acker J, Papa G, et al. Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses. <i>The EMBO Journal</i>. 2021;40(21). doi:<a href=\"https://doi.org/10.15252/embj.2021107711\">10.15252/embj.2021107711</a>","short":"F. Geiger, J. Acker, G. Papa, X. Wang, W.E. Arter, K.L. Saar, N.A. Erkamp, R. Qi, J.P.K. Bravo, S. Strauss, G. Krainer, O.R. Burrone, R. Jungmann, T.P. Knowles, H. Engelke, A. Borodavka, The EMBO Journal 40 (2021).","ieee":"F. Geiger <i>et al.</i>, “Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses,” <i>The EMBO Journal</i>, vol. 40, no. 21. Embo Press, 2021."},"quality_controlled":"1","day":"02","intvolume":"        40","doi":"10.15252/embj.2021107711","publisher":"Embo Press","type":"journal_article","publication_status":"published","oa_version":"Published Version","keyword":["General Immunology and Microbiology","General Biochemistry","Genetics and Molecular Biology","Molecular Biology","General Neuroscience"],"main_file_link":[{"url":"https://doi.org/10.15252/embj.2021107711","open_access":"1"}],"month":"11","article_type":"original","title":"Liquid–liquid phase separation underpins the formation of replication factories in rotaviruses","date_updated":"2024-06-04T06:08:16Z","publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"_id":"15138","pmid":1},{"month":"10","article_type":"original","publisher":"Embo Press","publication_status":"published","type":"journal_article","oa_version":"Published Version","_id":"10179","pmid":1,"isi":1,"publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"date_updated":"2023-08-14T08:05:23Z","title":"Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"department":[{"_id":"Bio"}],"file":[{"date_created":"2021-12-13T14:54:14Z","content_type":"application/pdf","access_level":"open_access","checksum":"78d2d02e775322297e774f72810a41a4","file_size":7819881,"file_name":"2021_EMBO_Bajaj.pdf","success":1,"relation":"main_file","file_id":"10541","creator":"alisjak","date_updated":"2021-12-13T14:54:14Z"}],"date_published":"2021-10-18T00:00:00Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","volume":40,"author":[{"last_name":"Bajaj","full_name":"Bajaj, Sunanjay","first_name":"Sunanjay"},{"first_name":"Joshua A.","full_name":"Bagley, Joshua A.","last_name":"Bagley"},{"id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","last_name":"Sommer","orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","first_name":"Christoph M"},{"full_name":"Vertesy, Abel","last_name":"Vertesy","first_name":"Abel"},{"first_name":"Sakurako","full_name":"Nagumo Wong, Sakurako","last_name":"Nagumo Wong"},{"first_name":"Veronica","full_name":"Krenn, Veronica","last_name":"Krenn"},{"last_name":"Lévi-Strauss","full_name":"Lévi-Strauss, Julie","first_name":"Julie"},{"first_name":"Juergen A.","last_name":"Knoblich","full_name":"Knoblich, Juergen A."}],"external_id":{"isi":["000708012800001"],"pmid":["34661293"]},"status":"public","acknowledgement":"We thank all Knoblich laboratory members for continued support and discussions. We thank the IMP/IMBA BioOptics facility, particularly Pawel Pasierbek, Alberto Moreno Cencerrado and Gerald Schmauss, the IMP/IMBA Molecular Biology Service, in particular Robert Heinen, the IMP Bioinformatics facility, in particular Thomas Burkard, the Vienna Biocenter Core Facilities (VBCF) Histopathology facility, in particular Tamara Engelmaier, and the VBCF Next Generation Sequencing Facility, notably Volodymyr Shubchynskyy and Carmen Czepe. We would also like to thank Simon Haendeler for advice on statistical analyses, Jose Guzman for discussions and assistance with slice culture setups, Oliver L. Eichmueller for discussions and assistance with microscopy, and E.H. Gustafson, S. Wolfinger, and D. Reumann for technical assistance regarding generation of cerebral organoids. This project received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie fellowship agreement Nr.707109 awarded to J.A.B. Work in J.A.K.'s laboratory is supported by the Austrian Federal Ministry of Education, Science and Research, the Austrian Academy of Sciences, the City of Vienna, a Research Program of the Austrian Science Fund FWF (SFBF78 Stem Cell, F 7803-B) and a European Research Council (ERC) Advanced Grant under the European 20 Union’s Horizon 2020 program (grant agreement no. 695642).","year":"2021","publication":"EMBO Journal","language":[{"iso":"eng"}],"issue":"23","intvolume":"        40","doi":"10.15252/embj.2021108714","citation":{"apa":"Bajaj, S., Bagley, J. A., Sommer, C. M., Vertesy, A., Nagumo Wong, S., Krenn, V., … Knoblich, J. A. (2021). Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2021108714\">https://doi.org/10.15252/embj.2021108714</a>","mla":"Bajaj, Sunanjay, et al. “Neurotransmitter Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.” <i>EMBO Journal</i>, vol. 40, no. 23, e108714, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2021108714\">10.15252/embj.2021108714</a>.","chicago":"Bajaj, Sunanjay, Joshua A. Bagley, Christoph M Sommer, Abel Vertesy, Sakurako Nagumo Wong, Veronica Krenn, Julie Lévi-Strauss, and Juergen A. Knoblich. “Neurotransmitter Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.” <i>EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2021108714\">https://doi.org/10.15252/embj.2021108714</a>.","ista":"Bajaj S, Bagley JA, Sommer CM, Vertesy A, Nagumo Wong S, Krenn V, Lévi-Strauss J, Knoblich JA. 2021. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. EMBO Journal. 40(23), e108714.","short":"S. Bajaj, J.A. Bagley, C.M. Sommer, A. Vertesy, S. Nagumo Wong, V. Krenn, J. Lévi-Strauss, J.A. Knoblich, EMBO Journal 40 (2021).","ieee":"S. Bajaj <i>et al.</i>, “Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration,” <i>EMBO Journal</i>, vol. 40, no. 23. Embo Press, 2021.","ama":"Bajaj S, Bagley JA, Sommer CM, et al. Neurotransmitter signaling regulates distinct phases of multimodal human interneuron migration. <i>EMBO Journal</i>. 2021;40(23). doi:<a href=\"https://doi.org/10.15252/embj.2021108714\">10.15252/embj.2021108714</a>"},"quality_controlled":"1","date_created":"2021-10-24T22:01:34Z","scopus_import":"1","day":"18","article_number":"e108714","article_processing_charge":"Yes (in subscription journal)","has_accepted_license":"1","abstract":[{"text":"Inhibitory GABAergic interneurons migrate over long distances from their extracortical origin into the developing cortex. In humans, this process is uniquely slow and prolonged, and it is unclear whether guidance cues unique to humans govern the various phases of this complex developmental process. Here, we use fused cerebral organoids to identify key roles of neurotransmitter signaling pathways in guiding the migratory behavior of human cortical interneurons. We use scRNAseq to reveal expression of GABA, glutamate, glycine, and serotonin receptors along distinct maturation trajectories across interneuron migration. We develop an image analysis software package, TrackPal, to simultaneously assess 48 parameters for entire migration tracks of individual cells. By chemical screening, we show that different modes of interneuron migration depend on distinct neurotransmitter signaling pathways, linking transcriptional maturation of interneurons with their migratory behavior. Altogether, our study provides a comprehensive quantitative analysis of human interneuron migration and its functional modulation by neurotransmitter signaling.","lang":"eng"}],"ddc":["610"],"oa":1,"file_date_updated":"2021-12-13T14:54:14Z"},{"oa_version":"Published Version","publication_status":"published","type":"journal_article","publisher":"Embo Press","article_type":"original","month":"02","project":[{"call_identifier":"FWF","grant_number":"I 1774-B16","name":"Hormone cross-talk drives nutrient dependent plant development","_id":"2542D156-B435-11E9-9278-68D0E5697425"},{"name":"Hormonal regulation of plant adaptive responses to environmental signals","_id":"2685A872-B435-11E9-9278-68D0E5697425"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","name":"Molecular mechanisms of endocytic cargo recognition in plants","grant_number":"I03630","call_identifier":"FWF"}],"date_updated":"2026-06-18T22:30:43Z","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"department":[{"_id":"JiFr"},{"_id":"EvBe"}],"title":"Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport","pmid":1,"_id":"9010","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"acknowledged_ssus":[{"_id":"Bio"}],"isi":1,"year":"2021","issue":"3","publication":"EMBO Journal","language":[{"iso":"eng"}],"volume":40,"author":[{"last_name":"Ötvös","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-5503-4983","full_name":"Ötvös, Krisztina","first_name":"Krisztina"},{"first_name":"Marco","last_name":"Marconi","full_name":"Marconi, Marco"},{"first_name":"Andrea","last_name":"Vega","full_name":"Vega, Andrea"},{"first_name":"Jose","last_name":"O’Brien","full_name":"O’Brien, Jose"},{"orcid":"0000-0002-2739-8843","full_name":"Johnson, Alexander J","last_name":"Johnson","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J"},{"id":"4827E134-F248-11E8-B48F-1D18A9856A87","last_name":"Abualia","full_name":"Abualia, Rashed","orcid":"0000-0002-9357-9415","first_name":"Rashed"},{"first_name":"Livio","full_name":"Antonielli, Livio","last_name":"Antonielli"},{"full_name":"Montesinos López, Juan C","orcid":"0000-0001-9179-6099","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","last_name":"Montesinos López","first_name":"Juan C"},{"first_name":"Yuzhou","last_name":"Zhang","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou"},{"first_name":"Shutang","orcid":"0000-0002-0471-8285","full_name":"Tan, Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","last_name":"Tan"},{"last_name":"Cuesta","id":"33A3C818-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-1923-2410","full_name":"Cuesta, Candela","first_name":"Candela"},{"first_name":"Christina","full_name":"Artner, Christina","last_name":"Artner","id":"45DF286A-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Eleonore","full_name":"Bouguyon, Eleonore","last_name":"Bouguyon"},{"full_name":"Gojon, Alain","last_name":"Gojon","first_name":"Alain"},{"first_name":"Jiří","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml"},{"full_name":"Gutiérrez, Rodrigo A.","last_name":"Gutiérrez","first_name":"Rodrigo A."},{"first_name":"Krzysztof T","orcid":"0000-0001-7263-0560","full_name":"Wabnik, Krzysztof T","id":"4DE369A4-F248-11E8-B48F-1D18A9856A87","last_name":"Wabnik"},{"full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","first_name":"Eva"}],"external_id":{"pmid":[" 33399250"],"isi":["000604645600001"]},"file":[{"date_updated":"2021-02-11T12:28:29Z","relation":"main_file","creator":"dernst","file_id":"9110","file_size":2358617,"success":1,"file_name":"2021_Embo_Otvos.pdf","content_type":"application/pdf","access_level":"open_access","date_created":"2021-02-11T12:28:29Z","checksum":"dc55c900f3b061d6c2790b8813d759a3"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","date_published":"2021-02-01T00:00:00Z","acknowledgement":"We acknowledge Gergely Molnar for critical reading of the manuscript, Alexander Johnson for language editing and Yulija Salanenka for technical assistance. Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to KO, RA and EB. Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S) to KO, RA and EB and by the DOC Fellowship Programme of the AustrianAcademy of Sciences (25008) to C.A. Work in the Wabnik laboratory was supported by the Programa de Atraccion de Talento 2017 (Comunidad deMadrid, 2017-T1/BIO-5654 to K.W.), Severo Ochoa Programme for Centres of Excellence in R&D from the Agencia Estatal de Investigacion of Spain (grantSEV-2016-0672 (2017-2021) to K.W. via the CBGP) and Programa Estatal de Generacion del Conocimiento y Fortalecimiento Científico y Tecnologico del Sistema de I+D+I 2019 (PGC2018-093387-A-I00) from MICIU (to K.W.). M.M.was supported by a postdoctoral contract associated to SEV-2016-0672.We acknowledge the Bioimaging Facility in IST-Austria and the Advanced Microscopy Facility of the Vienna Bio Center Core Facilities, member of the Vienna Bio Center Austria, for use of the OMX v43D SIM microscope. AJ was supported by the Austrian Science Fund (FWF): I03630 to J.F","status":"public","has_accepted_license":"1","article_number":"e106862","article_processing_charge":"Yes (via OA deal)","oa":1,"file_date_updated":"2021-02-11T12:28:29Z","ddc":["580"],"abstract":[{"text":"Availability of the essential macronutrient nitrogen in soil plays a critical role in plant growth, development, and impacts agricultural productivity. Plants have evolved different strategies for sensing and responding to heterogeneous nitrogen distribution. Modulation of root system architecture, including primary root growth and branching, is among the most essential plant adaptions to ensure adequate nitrogen acquisition. However, the immediate molecular pathways coordinating the adjustment of root growth in response to distinct nitrogen sources, such as nitrate or ammonium, are poorly understood. Here, we show that growth as manifested by cell division and elongation is synchronized by coordinated auxin flux between two adjacent outer tissue layers of the root. This coordination is achieved by nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization and thereby regulating auxin flow between adjacent tissues. A dynamic computer model based on our experimental data successfully recapitulates experimental observations. Our study provides mechanistic insights broadening our understanding of root growth mechanisms in dynamic environments.","lang":"eng"}],"doi":"10.15252/embj.2020106862","related_material":{"link":[{"relation":"press_release","url":"https://ist.ac.at/en/news/a-plants-way-to-its-favorite-food/","description":"News on IST Homepage"}],"record":[{"relation":"dissertation_contains","status":"public","id":"10303"}]},"intvolume":"        40","corr_author":"1","day":"01","date_created":"2021-01-17T23:01:12Z","quality_controlled":"1","citation":{"ama":"Ötvös K, Marconi M, Vega A, et al. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. <i>EMBO Journal</i>. 2021;40(3). doi:<a href=\"https://doi.org/10.15252/embj.2020106862\">10.15252/embj.2020106862</a>","short":"K. Ötvös, M. Marconi, A. Vega, J. O’Brien, A.J. Johnson, R. Abualia, L. Antonielli, J.C. Montesinos López, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A. Gojon, J. Friml, R.A. Gutiérrez, K.T. Wabnik, E. Benková, EMBO Journal 40 (2021).","ieee":"K. Ötvös <i>et al.</i>, “Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport,” <i>EMBO Journal</i>, vol. 40, no. 3. Embo Press, 2021.","apa":"Ötvös, K., Marconi, M., Vega, A., O’Brien, J., Johnson, A. J., Abualia, R., … Benková, E. (2021). Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. <i>EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2020106862\">https://doi.org/10.15252/embj.2020106862</a>","chicago":"Ötvös, Krisztina, Marco Marconi, Andrea Vega, Jose O’Brien, Alexander J Johnson, Rashed Abualia, Livio Antonielli, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” <i>EMBO Journal</i>. Embo Press, 2021. <a href=\"https://doi.org/10.15252/embj.2020106862\">https://doi.org/10.15252/embj.2020106862</a>.","mla":"Ötvös, Krisztina, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” <i>EMBO Journal</i>, vol. 40, no. 3, e106862, Embo Press, 2021, doi:<a href=\"https://doi.org/10.15252/embj.2020106862\">10.15252/embj.2020106862</a>.","ista":"Ötvös K, Marconi M, Vega A, O’Brien J, Johnson AJ, Abualia R, Antonielli L, Montesinos López JC, Zhang Y, Tan S, Cuesta C, Artner C, Bouguyon E, Gojon A, Friml J, Gutiérrez RA, Wabnik KT, Benková E. 2021. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport. EMBO Journal. 40(3), e106862."},"scopus_import":"1"},{"date_updated":"2025-04-15T06:37:27Z","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"department":[{"_id":"MiSi"},{"_id":"EvBe"}],"title":"Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage","pmid":1,"_id":"8142","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"isi":1,"acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"}],"oa_version":"Published Version","type":"journal_article","publisher":"Embo Press","publication_status":"published","article_type":"original","month":"09","project":[{"name":"Molecular mechanism of auxindriven formative divisions delineating lateral root organogenesis in plants","grant_number":"ALTF710-2016","_id":"253E54C8-B435-11E9-9278-68D0E5697425"},{"_id":"2542D156-B435-11E9-9278-68D0E5697425","name":"Hormone cross-talk drives nutrient dependent plant development","grant_number":"I 1774-B16","call_identifier":"FWF"}],"has_accepted_license":"1","article_number":"e104238","article_processing_charge":"Yes (via OA deal)","oa":1,"file_date_updated":"2020-12-02T09:13:23Z","abstract":[{"lang":"eng","text":"Cell production and differentiation for the acquisition of specific functions are key features of living systems. The dynamic network of cellular microtubules provides the necessary platform to accommodate processes associated with the transition of cells through the individual phases of cytogenesis. Here, we show that the plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton during cell differentiation and counteracts microtubular rearrangements driven by the hormone auxin. The endogenous upward gradient of cytokinin activity along the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust rearrangements of the microtubule cytoskeleton in epidermal cells progressing from the proliferative to the differentiation stage. Controlled increases in cytokinin activity result in premature re‐organization of the microtubule network from transversal to an oblique disposition in cells prior to their differentiation, whereas attenuated hormone perception delays cytoskeleton conversion into a configuration typical for differentiated cells. Intriguingly, cytokinin can interfere with microtubules also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive control pathway for the microtubular cytoskeleton may be at least partially conserved between plant and animal cells."}],"ddc":["580"],"doi":"10.15252/embj.2019104238","intvolume":"        39","day":"01","corr_author":"1","citation":{"ista":"Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. The Embo Journal. 39(17), e104238.","apa":"Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös, K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>","mla":"Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>, vol. 39, no. 17, e104238, Embo Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>.","chicago":"Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia, Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated Stage.” <i>The Embo Journal</i>. Embo Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019104238\">https://doi.org/10.15252/embj.2019104238</a>.","short":"J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös, J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).","ieee":"J. C. Montesinos López <i>et al.</i>, “Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage,” <i>The Embo Journal</i>, vol. 39, no. 17. Embo Press, 2020.","ama":"Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides microtubule dynamics during cell progression from proliferative to differentiated stage. <i>The Embo Journal</i>. 2020;39(17). doi:<a href=\"https://doi.org/10.15252/embj.2019104238\">10.15252/embj.2019104238</a>"},"date_created":"2020-07-21T09:08:38Z","quality_controlled":"1","scopus_import":"1","year":"2020","issue":"17","publication":"The Embo Journal","language":[{"iso":"eng"}],"volume":39,"external_id":{"pmid":["32667089"],"isi":["000548311800001"]},"author":[{"last_name":"Montesinos López","id":"310A8E3E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-9179-6099","full_name":"Montesinos López, Juan C","first_name":"Juan C"},{"first_name":"A","full_name":"Abuzeineh, A","last_name":"Abuzeineh"},{"orcid":"0000-0002-2187-6656","full_name":"Kopf, Aglaja","last_name":"Kopf","id":"31DAC7B6-F248-11E8-B48F-1D18A9856A87","first_name":"Aglaja"},{"orcid":"0000-0002-1009-9652","full_name":"Juanes Garcia, Alba","id":"40F05888-F248-11E8-B48F-1D18A9856A87","last_name":"Juanes Garcia","first_name":"Alba"},{"first_name":"Krisztina","full_name":"Ötvös, Krisztina","orcid":"0000-0002-5503-4983","id":"29B901B0-F248-11E8-B48F-1D18A9856A87","last_name":"Ötvös"},{"first_name":"J","full_name":"Petrášek, J","last_name":"Petrášek"},{"first_name":"Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179","full_name":"Sixt, Michael K"},{"last_name":"Benková","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","first_name":"Eva"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_published":"2020-09-01T00:00:00Z","file":[{"file_size":3497156,"success":1,"file_name":"2020_EMBO_Montesinos.pdf","date_created":"2020-12-02T09:13:23Z","access_level":"open_access","content_type":"application/pdf","checksum":"43d2b36598708e6ab05c69074e191d57","date_updated":"2020-12-02T09:13:23Z","relation":"main_file","file_id":"8827","creator":"dernst"}],"acknowledgement":"We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring discussions, and Martine De Cock for help in preparing the manuscript. This research was supported by the Scientific Service Units (SSUs) of IST Austria through resources provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship (ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738 to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.","status":"public"},{"month":"03","article_type":"original","type":"journal_article","publisher":"EMBO Press","publication_status":"published","oa_version":"Published Version","_id":"7586","pmid":1,"isi":1,"publication_identifier":{"issn":["0261-4189"],"eissn":["1460-2075"]},"date_updated":"2026-04-16T09:35:48Z","tmp":{"image":"/images/cc_by_nc_nd.png","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","short":"CC BY-NC-ND (4.0)"},"department":[{"_id":"GaNo"}],"title":"Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration","date_published":"2020-03-02T00:00:00Z","file":[{"relation":"main_file","file_id":"7615","creator":"dernst","date_updated":"2020-07-14T12:48:00Z","date_created":"2020-03-23T13:51:11Z","access_level":"open_access","content_type":"application/pdf","checksum":"82750a7a93e3740decbce8474004111a","file_size":12243278,"file_name":"2020_EMBO_Weinert.pdf"}],"user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","external_id":{"isi":["000517335000001"],"pmid":["32118314"]},"author":[{"first_name":"Stefanie","last_name":"Weinert","full_name":"Weinert, Stefanie"},{"first_name":"Niclas","full_name":"Gimber, Niclas","last_name":"Gimber"},{"last_name":"Deuschel","full_name":"Deuschel, Dorothea","first_name":"Dorothea"},{"full_name":"Stuhlmann, Till","last_name":"Stuhlmann","first_name":"Till"},{"last_name":"Puchkov","full_name":"Puchkov, Dmytro","first_name":"Dmytro"},{"first_name":"Zohreh","last_name":"Farsi","full_name":"Farsi, Zohreh"},{"first_name":"Carmen F.","full_name":"Ludwig, Carmen F.","last_name":"Ludwig"},{"first_name":"Gaia","orcid":"0000-0002-7673-7178","full_name":"Novarino, Gaia","id":"3E57A680-F248-11E8-B48F-1D18A9856A87","last_name":"Novarino"},{"full_name":"López-Cayuqueo, Karen I.","last_name":"López-Cayuqueo","first_name":"Karen I."},{"full_name":"Planells-Cases, Rosa","last_name":"Planells-Cases","first_name":"Rosa"},{"first_name":"Thomas J.","full_name":"Jentsch, Thomas J.","last_name":"Jentsch"}],"volume":39,"acknowledgement":"We thank T. Stauber and T. Breiderhoff for cloning expression constructs; K. Räbel, S. Hohensee, and C. Backhaus for technical assistance; R. Jahn (MPIbpc, Göttingen) for providing the equipment required for SV purification; and A\r\nWoehler (MDC, Berlin) for assistance with SV imaging. Supported, in part, by grants from the Deutsche Forschungsgemeinschaft (JE164/9-2, SFB740 TP C5, FOR 2625 (JE164/14-1), NeuroCure Cluster of Excellence), the European Research Council Advanced Grant CYTOVOLION (ERC 294435) and the Prix Louis-Jeantet de Médecine to TJJ, and Peter and Traudl Engelhorn fellowship to ZF.","status":"public","year":"2020","publication":"EMBO Journal","language":[{"iso":"eng"}],"intvolume":"        39","doi":"10.15252/embj.2019103358","scopus_import":"1","quality_controlled":"1","citation":{"ista":"Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF, Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ. 2020. Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration. EMBO Journal. 39, e103358.","chicago":"Weinert, Stefanie, Niclas Gimber, Dorothea Deuschel, Till Stuhlmann, Dmytro Puchkov, Zohreh Farsi, Carmen F. Ludwig, et al. “Uncoupling Endosomal CLC Chloride/Proton Exchange Causes Severe Neurodegeneration.” <i>EMBO Journal</i>. EMBO Press, 2020. <a href=\"https://doi.org/10.15252/embj.2019103358\">https://doi.org/10.15252/embj.2019103358</a>.","apa":"Weinert, S., Gimber, N., Deuschel, D., Stuhlmann, T., Puchkov, D., Farsi, Z., … Jentsch, T. J. (2020). Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration. <i>EMBO Journal</i>. EMBO Press. <a href=\"https://doi.org/10.15252/embj.2019103358\">https://doi.org/10.15252/embj.2019103358</a>","mla":"Weinert, Stefanie, et al. “Uncoupling Endosomal CLC Chloride/Proton Exchange Causes Severe Neurodegeneration.” <i>EMBO Journal</i>, vol. 39, e103358, EMBO Press, 2020, doi:<a href=\"https://doi.org/10.15252/embj.2019103358\">10.15252/embj.2019103358</a>.","ieee":"S. Weinert <i>et al.</i>, “Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration,” <i>EMBO Journal</i>, vol. 39. EMBO Press, 2020.","short":"S. Weinert, N. Gimber, D. Deuschel, T. Stuhlmann, D. Puchkov, Z. Farsi, C.F. Ludwig, G. Novarino, K.I. López-Cayuqueo, R. Planells-Cases, T.J. Jentsch, EMBO Journal 39 (2020).","ama":"Weinert S, Gimber N, Deuschel D, et al. Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration. <i>EMBO Journal</i>. 2020;39. doi:<a href=\"https://doi.org/10.15252/embj.2019103358\">10.15252/embj.2019103358</a>"},"date_created":"2020-03-15T23:00:55Z","day":"02","article_processing_charge":"No","article_number":"e103358","has_accepted_license":"1","ddc":["570"],"abstract":[{"text":"CLC chloride/proton exchangers may support acidification of endolysosomes and raise their luminal Cl− concentration. Disruption of endosomal ClC‐3 causes severe neurodegeneration. To assess the importance of ClC‐3 Cl−/H+ exchange, we now generate Clcn3unc/unc mice in which ClC‐3 is converted into a Cl− channel. Unlike Clcn3−/− mice, Clcn3unc/unc mice appear normal owing to compensation by ClC‐4 with which ClC‐3 forms heteromers. ClC‐4 protein levels are strongly reduced in Clcn3−/−, but not in Clcn3unc/unc mice because ClC‐3unc binds and stabilizes ClC‐4 like wild‐type ClC‐3. Although mice lacking ClC‐4 appear healthy, its absence in Clcn3unc/unc/Clcn4−/− mice entails even stronger neurodegeneration than observed in Clcn3−/− mice. A fraction of ClC‐3 is found on synaptic vesicles, but miniature postsynaptic currents and synaptic vesicle acidification are not affected in Clcn3unc/unc or Clcn3−/− mice before neurodegeneration sets in. Both, Cl−/H+‐exchange activity and the stabilizing effect on ClC‐4, are central to the biological function of ClC‐3.","lang":"eng"}],"file_date_updated":"2020-07-14T12:48:00Z","oa":1},{"doi":"10.15252/embj.2019102497","intvolume":"        38","day":"15","corr_author":"1","scopus_import":"1","citation":{"ieee":"N. Petridou and C.-P. J. Heisenberg, “Tissue rheology in embryonic organization,” <i>The EMBO Journal</i>, vol. 38, no. 20. Embo Press, 2019.","short":"N. Petridou, C.-P.J. Heisenberg, The EMBO Journal 38 (2019).","ama":"Petridou N, Heisenberg C-PJ. Tissue rheology in embryonic organization. <i>The EMBO Journal</i>. 2019;38(20). doi:<a href=\"https://doi.org/10.15252/embj.2019102497\">10.15252/embj.2019102497</a>","ista":"Petridou N, Heisenberg C-PJ. 2019. Tissue rheology in embryonic organization. The EMBO Journal. 38(20), e102497.","apa":"Petridou, N., &#38; Heisenberg, C.-P. J. (2019). Tissue rheology in embryonic organization. <i>The EMBO Journal</i>. Embo Press. <a href=\"https://doi.org/10.15252/embj.2019102497\">https://doi.org/10.15252/embj.2019102497</a>","mla":"Petridou, Nicoletta, and Carl-Philipp J. Heisenberg. “Tissue Rheology in Embryonic Organization.” <i>The EMBO Journal</i>, vol. 38, no. 20, e102497, Embo Press, 2019, doi:<a href=\"https://doi.org/10.15252/embj.2019102497\">10.15252/embj.2019102497</a>.","chicago":"Petridou, Nicoletta, and Carl-Philipp J Heisenberg. “Tissue Rheology in Embryonic Organization.” <i>The EMBO Journal</i>. Embo Press, 2019. <a href=\"https://doi.org/10.15252/embj.2019102497\">https://doi.org/10.15252/embj.2019102497</a>."},"quality_controlled":"1","date_created":"2019-11-04T15:24:29Z","has_accepted_license":"1","article_processing_charge":"Yes (via OA deal)","article_number":"e102497","file_date_updated":"2020-07-14T12:47:46Z","oa":1,"ddc":["570"],"abstract":[{"text":"Tissue morphogenesis in multicellular organisms is brought about by spatiotemporal coordination of mechanical and chemical signals. Extensive work on how mechanical forces together with the well‐established morphogen signalling pathways can actively shape living tissues has revealed evolutionary conserved mechanochemical features of embryonic development. More recently, attention has been drawn to the description of tissue material properties and how they can influence certain morphogenetic processes. Interestingly, besides the role of tissue material properties in determining how much tissues deform in response to force application, there is increasing theoretical and experimental evidence, suggesting that tissue material properties can abruptly and drastically change in development. These changes resemble phase transitions, pointing at the intriguing possibility that important morphogenetic processes in development, such as symmetry breaking and self‐organization, might be mediated by tissue phase transitions. In this review, we summarize recent findings on the regulation and role of tissue material properties in the context of the developing embryo. We posit that abrupt changes of tissue rheological properties may have important implications in maintaining the balance between robustness and adaptability during embryonic development.","lang":"eng"}],"external_id":{"isi":["000485561900001"],"pmid":["31512749"]},"author":[{"first_name":"Nicoletta","last_name":"Petridou","id":"2A003F6C-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8451-1195","full_name":"Petridou, Nicoletta"},{"last_name":"Heisenberg","id":"39427864-F248-11E8-B48F-1D18A9856A87","full_name":"Heisenberg, Carl-Philipp J","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"}],"volume":38,"file":[{"creator":"dernst","file_id":"6981","relation":"main_file","date_updated":"2020-07-14T12:47:46Z","checksum":"76f7f4e79ab6d850c30017a69726fd85","access_level":"open_access","content_type":"application/pdf","date_created":"2019-11-04T15:30:08Z","file_name":"2019_Embo_Petridou.pdf","file_size":847356}],"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","date_published":"2019-10-15T00:00:00Z","status":"public","year":"2019","issue":"20","publication":"The EMBO Journal","language":[{"iso":"eng"}],"pmid":1,"ec_funded":1,"_id":"6980","publication_identifier":{"eissn":["1460-2075"],"issn":["0261-4189"]},"isi":1,"date_updated":"2025-05-14T11:21:32Z","title":"Tissue rheology in embryonic organization","tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"department":[{"_id":"CaHe"}],"article_type":"review","project":[{"call_identifier":"H2020","grant_number":"742573","name":"Interaction and feedback between cell mechanics and fate specification in vertebrate gastrulation","_id":"260F1432-B435-11E9-9278-68D0E5697425"},{"name":"Tissue material properties in embryonic development","grant_number":"V00736","call_identifier":"FWF","_id":"2693FD8C-B435-11E9-9278-68D0E5697425"}],"month":"10","oa_version":"Published Version","type":"journal_article","publisher":"Embo Press","publication_status":"published"},{"department":[{"_id":"JoDa"}],"tmp":{"image":"/images/cc_by.png","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)"},"title":"Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission","date_updated":"2024-10-09T20:58:32Z","isi":1,"publication_identifier":{"issn":["0261-4189"]},"_id":"145","pmid":1,"publication_status":"published","type":"journal_article","publisher":"Wiley","oa_version":"Published Version","month":"08","article_type":"original","abstract":[{"text":"Aged proteins can become hazardous to cellular function, by accumulating molecular damage. This implies that cells should preferentially rely on newly produced ones. We tested this hypothesis in cultured hippocampal neurons, focusing on synaptic transmission. We found that newly synthesized vesicle proteins were incorporated in the actively recycling pool of vesicles responsible for all neurotransmitter release during physiological activity. We observed this for the calcium sensor Synaptotagmin 1, for the neurotransmitter transporter VGAT, and for the fusion protein VAMP2 (Synaptobrevin 2). Metabolic labeling of proteins and visualization by secondary ion mass spectrometry enabled us to query the entire protein makeup of the actively recycling vesicles, which we found to be younger than that of non-recycling vesicles. The young vesicle proteins remained in use for up to ~ 24 h, during which they participated in recycling a few hundred times. They were afterward reluctant to release and were degraded after an additional ~ 24–48 h. We suggest that the recycling pool of synaptic vesicles relies on newly synthesized proteins, while the inactive reserve pool contains older proteins.","lang":"eng"}],"ddc":["570"],"oa":1,"file_date_updated":"2020-07-14T12:44:56Z","article_number":"e98044","article_processing_charge":"No","has_accepted_license":"1","date_created":"2018-12-11T11:44:52Z","quality_controlled":"1","citation":{"ista":"Truckenbrodt SM, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero E, Rizzoli S. 2018. Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission. The EMBO Journal. 37(15), e98044.","mla":"Truckenbrodt, Sven M., et al. “Newly Produced Synaptic Vesicle Proteins Are Preferentially Used in Synaptic Transmission.” <i>The EMBO Journal</i>, vol. 37, no. 15, e98044, Wiley, 2018, doi:<a href=\"https://doi.org/10.15252/embj.201798044\">10.15252/embj.201798044</a>.","apa":"Truckenbrodt, S. M., Viplav, A., Jähne, S., Vogts, A., Denker, A., Wildhagen, H., … Rizzoli, S. (2018). Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission. <i>The EMBO Journal</i>. Wiley. <a href=\"https://doi.org/10.15252/embj.201798044\">https://doi.org/10.15252/embj.201798044</a>","chicago":"Truckenbrodt, Sven M, Abhiyan Viplav, Sebsatian Jähne, Angela Vogts, Annette Denker, Hanna Wildhagen, Eugenio Fornasiero, and Silvio Rizzoli. “Newly Produced Synaptic Vesicle Proteins Are Preferentially Used in Synaptic Transmission.” <i>The EMBO Journal</i>. Wiley, 2018. <a href=\"https://doi.org/10.15252/embj.201798044\">https://doi.org/10.15252/embj.201798044</a>.","ama":"Truckenbrodt SM, Viplav A, Jähne S, et al. Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission. <i>The EMBO Journal</i>. 2018;37(15). doi:<a href=\"https://doi.org/10.15252/embj.201798044\">10.15252/embj.201798044</a>","short":"S.M. Truckenbrodt, A. Viplav, S. Jähne, A. Vogts, A. Denker, H. Wildhagen, E. Fornasiero, S. Rizzoli, The EMBO Journal 37 (2018).","ieee":"S. M. Truckenbrodt <i>et al.</i>, “Newly produced synaptic vesicle proteins are preferentially used in synaptic transmission,” <i>The EMBO Journal</i>, vol. 37, no. 15. Wiley, 2018."},"scopus_import":"1","corr_author":"1","day":"01","intvolume":"        37","doi":"10.15252/embj.201798044","publication":"The EMBO Journal","language":[{"iso":"eng"}],"issue":"15","year":"2018","acknowledgement":"We thank Reinhard Jahn for providing a plasmid for YFP-SNAP25. We thank Erwin Neher for help with the development of the mathematical model of the synaptic vesicle life cycle. We thank Martin Meschkat, Andreas Höbartner, Annedore Punge, and Peer Hoopmann for help with the experiments. We thank Burkhard Rammner for providing the illustrations of synaptic vesicle and protein dynamics. We thank Manuel Maidorn, Martin Helm, and Katharina N. Richter for critically reading the manuscript. S.T. was supported by an Excellence Stipend of the Göttingen Graduate School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB). E.F.F. is a recipient of long-term fellowships from the European Molecular Biology Organization (ALTF_797-2012) and from the Human Frontier Science Program (HFSP_LT000830/2013). The work was supported by grants to S.O.R. from the European Research Council (ERC-2013-CoG NeuroMolAnatomy) and from the Deutsche Forschungsgemeinschaft (Cluster of Excellence Nanoscale Microscopy and Molecular Physiology of the Brain, SFB1190/P09, SFB889/A05, and SFB1286/A03, and DFG RI 1967 7/1). The nanoSIMS instrument was funded by the German Federal Ministry of Education and Research (03F0626A).","status":"public","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","date_published":"2018-08-01T00:00:00Z","file":[{"file_id":"5710","creator":"dernst","relation":"main_file","date_updated":"2020-07-14T12:44:56Z","checksum":"a540feb6c9af6aefc78de531461a8835","date_created":"2018-12-17T14:17:29Z","content_type":"application/pdf","access_level":"open_access","file_name":"2018_EMBO_Truckenbrodt.pdf","file_size":2846470}],"publist_id":"7778","volume":37,"external_id":{"isi":["000440416900005"],"pmid":["29950309"]},"author":[{"last_name":"Truckenbrodt","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","full_name":"Truckenbrodt, Sven M","first_name":"Sven M"},{"last_name":"Viplav","full_name":"Viplav, Abhiyan","first_name":"Abhiyan"},{"first_name":"Sebsatian","full_name":"Jähne, Sebsatian","last_name":"Jähne"},{"first_name":"Angela","last_name":"Vogts","full_name":"Vogts, Angela"},{"full_name":"Denker, Annette","last_name":"Denker","first_name":"Annette"},{"first_name":"Hanna","last_name":"Wildhagen","full_name":"Wildhagen, Hanna"},{"full_name":"Fornasiero, Eugenio","last_name":"Fornasiero","first_name":"Eugenio"},{"first_name":"Silvio","full_name":"Rizzoli, Silvio","last_name":"Rizzoli"}]},{"year":"2001","issue":"11","language":[{"iso":"eng"}],"publication":"EMBO Journal","page":"2779 - 2788","author":[{"first_name":"Arthur","full_name":"Molendijk, Arthur","last_name":"Molendijk"},{"last_name":"Bischoff","full_name":"Bischoff, Friedrich","first_name":"Friedrich"},{"last_name":"Rajendrakumar","full_name":"Rajendrakumar, Chadalavada","first_name":"Chadalavada"},{"last_name":"Friml","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","first_name":"Jirí"},{"first_name":"Markus","last_name":"Braun","full_name":"Braun, Markus"},{"first_name":"Simon","last_name":"Gilroy","full_name":"Gilroy, Simon"},{"full_name":"Palme, Klaus","last_name":"Palme","first_name":"Klaus"}],"external_id":{"pmid":["11387211"]},"publist_id":"3721","extern":"1","volume":20,"user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"2001-06-01T00:00:00Z","acknowledgement":"We thank Drs Frantisek Baluška, Matthias Godde, Peter Huijser, Lars Vahlkamp and Dieter Volkmann for help, criticism and constructive reading of the manuscript. We are grateful to Dr N.-H.Chua for providing us with pTA7002. The work was funded by the DFG, the European Communities Biotechnology Programme (Bio4-CT98 0239) and the INCO Copernicus Programme (IC15-CT96-0920). C.S.V.R. is the recipient of an Alexander von Humboldt fellowship and J.F. of a DAAD fellowship.","status":"public","article_processing_charge":"No","oa":1,"abstract":[{"lang":"eng","text":"Plants contain a novel unique subfamily of Rho GTPases, vital components of cellular signalling networks. Here we report a general role for some members of this family in polarized plant growth processes. We show that Arabidopsis AtRop4 and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates. We localized AtRop proteins in root meristem cells to the cross-wall and cell plate membranes. Polar localization of AtRops in trichoblasts specifies the growth sites for emerging root hairs. These sites were visible before budding and elongation of the Arabidopsis root hair when AtRops accumulated at their tips. Expression of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic Arabidopsis plants abolished polarized growth and delocalized the tip-focused Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse growth."}],"doi":"10.1093/emboj/20.11.2779","intvolume":"        20","day":"01","scopus_import":"1","citation":{"ama":"Molendijk A, Bischoff F, Rajendrakumar C, et al. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. <i>EMBO Journal</i>. 2001;20(11):2779-2788. doi:<a href=\"https://doi.org/10.1093/emboj/20.11.2779\">10.1093/emboj/20.11.2779</a>","ieee":"A. Molendijk <i>et al.</i>, “Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth,” <i>EMBO Journal</i>, vol. 20, no. 11. Wiley-Blackwell, pp. 2779–2788, 2001.","short":"A. Molendijk, F. Bischoff, C. Rajendrakumar, J. Friml, M. Braun, S. Gilroy, K. Palme, EMBO Journal 20 (2001) 2779–2788.","ista":"Molendijk A, Bischoff F, Rajendrakumar C, Friml J, Braun M, Gilroy S, Palme K. 2001. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. EMBO Journal. 20(11), 2779–2788.","chicago":"Molendijk, Arthur, Friedrich Bischoff, Chadalavada Rajendrakumar, Jiří Friml, Markus Braun, Simon Gilroy, and Klaus Palme. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” <i>EMBO Journal</i>. Wiley-Blackwell, 2001. <a href=\"https://doi.org/10.1093/emboj/20.11.2779\">https://doi.org/10.1093/emboj/20.11.2779</a>.","mla":"Molendijk, Arthur, et al. “Arabidopsis Thaliana Rop GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” <i>EMBO Journal</i>, vol. 20, no. 11, Wiley-Blackwell, 2001, pp. 2779–88, doi:<a href=\"https://doi.org/10.1093/emboj/20.11.2779\">10.1093/emboj/20.11.2779</a>.","apa":"Molendijk, A., Bischoff, F., Rajendrakumar, C., Friml, J., Braun, M., Gilroy, S., &#38; Palme, K. (2001). Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1093/emboj/20.11.2779\">https://doi.org/10.1093/emboj/20.11.2779</a>"},"quality_controlled":"1","date_created":"2018-12-11T12:00:40Z","oa_version":"Published Version","publication_status":"published","type":"journal_article","publisher":"Wiley-Blackwell","article_type":"original","month":"06","main_file_link":[{"url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125484/","open_access":"1"}],"date_updated":"2023-05-16T12:07:45Z","title":"Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control polar growth","pmid":1,"_id":"2981","publication_identifier":{"issn":["0261-4189"]}},{"status":"public","acknowledgement":"We thank Professor Süss (Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany) for the gift of the anti-FNR antiserum, Professor Masahiro Sugiura (Nagoya University, Japan) for the gift of plasmid pTB19 and Professor Peter Horton (University of Sheffield) for the loan of his ED-800T unit. P.B. is a recipient of a BBSRC studentship and the work was supported by grants from the BBSRC, The Royal Society (to P.J.N.) and The National Science Foundation (to P.M.).","author":[{"last_name":"Burrows","full_name":"Burrows, Paul","first_name":"Paul"},{"first_name":"Leonid A","orcid":"0000-0002-0977-7989","full_name":"Sazanov, Leonid A","id":"338D39FE-F248-11E8-B48F-1D18A9856A87","last_name":"Sazanov"},{"full_name":"Sváb, Zóra","last_name":"Sváb","first_name":"Zóra"},{"last_name":"Maliga","full_name":"Maliga, Pàl","first_name":"Pàl"},{"first_name":"Peter","last_name":"Nixon","full_name":"Nixon, Peter"}],"external_id":{"pmid":["9463365"]},"publist_id":"5129","volume":17,"extern":"1","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","date_published":"1998-02-04T00:00:00Z","issue":"4","publication":"EMBO Journal","language":[{"iso":"eng"}],"page":"868 - 876","year":"1998","day":"04","scopus_import":"1","citation":{"chicago":"Burrows, Paul, Leonid A Sazanov, Zóra Sváb, Pàl Maliga, and Peter Nixon. “Identification of a Functional Respiratory Complex in Chloroplasts through Analysis of Tobacco Mutants Containing Disrupted Plastid Ndh Genes.” <i>EMBO Journal</i>. Wiley-Blackwell, 1998. <a href=\"https://doi.org/10.1093/emboj/17.4.868\">https://doi.org/10.1093/emboj/17.4.868</a>.","apa":"Burrows, P., Sazanov, L. A., Sváb, Z., Maliga, P., &#38; Nixon, P. (1998). Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. <i>EMBO Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1093/emboj/17.4.868\">https://doi.org/10.1093/emboj/17.4.868</a>","mla":"Burrows, Paul, et al. “Identification of a Functional Respiratory Complex in Chloroplasts through Analysis of Tobacco Mutants Containing Disrupted Plastid Ndh Genes.” <i>EMBO Journal</i>, vol. 17, no. 4, Wiley-Blackwell, 1998, pp. 868–76, doi:<a href=\"https://doi.org/10.1093/emboj/17.4.868\">10.1093/emboj/17.4.868</a>.","ista":"Burrows P, Sazanov LA, Sváb Z, Maliga P, Nixon P. 1998. Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. EMBO Journal. 17(4), 868–876.","ama":"Burrows P, Sazanov LA, Sváb Z, Maliga P, Nixon P. Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. <i>EMBO Journal</i>. 1998;17(4):868-876. doi:<a href=\"https://doi.org/10.1093/emboj/17.4.868\">10.1093/emboj/17.4.868</a>","short":"P. Burrows, L.A. Sazanov, Z. Sváb, P. Maliga, P. Nixon, EMBO Journal 17 (1998) 868–876.","ieee":"P. Burrows, L. A. Sazanov, Z. Sváb, P. Maliga, and P. Nixon, “Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes,” <i>EMBO Journal</i>, vol. 17, no. 4. Wiley-Blackwell, pp. 868–876, 1998."},"quality_controlled":"1","date_created":"2018-12-11T11:54:54Z","doi":"10.1093/emboj/17.4.868","intvolume":"        17","oa":1,"abstract":[{"lang":"eng","text":"The plastid genomes of several plants contain homologues, termed ndh genes, of genes encoding subunits of the NADH:ubiquinone oxidoreductase or complex I of mitochondria and eubacteria. The functional significance of the Ndh proteins in higher plants is uncertain. We show here that tobacco chloroplasts contain a protein complex of 550 kDa consisting of at least three of the ndh gene products: NdhI, NdhJ and NdhK. We have constructed mutant tobacco plants with disrupted ndhC, ndhK and ndhJ plastid genes, indicating that the Ndh complex is dispensible for plant growth under optimal growth conditions. Chlorophyll fluorescence analysis shows that in vivo the Ndh complex catalyses the post-illumination reduction of the plastoquinone pool and in the light optimizes the induction of photosynthesis under conditions of water stress. We conclude that the Ndh complex catalyses the reduction of the plastoquinone pool using stromal reductant and so acts as a respiratory complex. Overall, our data are compatible with the participation of the Ndh complex in cyclic electron flow around the photosystem I complex in the light and possibly in a chloroplast respiratory chain in the dark."}],"article_processing_charge":"No","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170436/"}],"article_type":"original","month":"02","oa_version":"None","publisher":"Wiley-Blackwell","type":"journal_article","publication_status":"published","publication_identifier":{"issn":["0261-4189"]},"pmid":1,"_id":"1955","title":"Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes","date_updated":"2022-09-01T13:17:49Z"}]