---
OA_place: publisher
OA_type: gold
_id: '18073'
abstract:
- lang: eng
text: Conserved signaling cascades monitor protein-folding homeostasis to ensure
proper cellular function. One of the evolutionary conserved key players is IRE1,
which maintains endoplasmic reticulum (ER) homeostasis through the unfolded protein
response (UPR). Upon accumulation of misfolded proteins in the ER, IRE1 forms
clusters on the ER membrane to initiate UPR signaling. What regulates IRE1 cluster
formation is not fully understood. Here, we show that the ER lumenal domain (LD)
of human IRE1α forms biomolecular condensates in vitro. IRE1α LD condensates were
stabilized both by binding to unfolded polypeptides as well as by tethering to
model membranes, suggesting their role in assembling IRE1α into signaling-competent
stable clusters. Molecular dynamics simulations indicated that weak multivalent
interactions drive IRE1α LD clustering. Mutagenesis experiments identified disordered
regions in IRE1α LD to control its clustering in vitro and in cells. Importantly,
dysregulated clustering of IRE1α mutants led to defects in IRE1α signaling. Our
results revealed that disordered regions in IRE1α LD control its clustering and
suggest their role as a common strategy in regulating protein assembly on membranes.
acknowledgement: We thank late Thomas Peterbauer at the Max Perutz Labs Biooptics
Light Microscopy Facility for his help and support. We are grateful to Kitti Csalyi
and Thomas Sauer at Max Perutz Labs Biooptics FACS facility for their help. We thank
Grzegorz Scibisz and Sertan Atilla for their support with the expression and purification
of mCherry-IRE1α LD-10His. We are grateful to Aleksandra S Anisimova with her help
in the generation of stable cell lines and the statistical analyses of the data.
We thank Venja Vieweger for her help with the characterization of the WLLI and D123P
IRE1 mutants in cells. We are thankful to Monika Kubickova for the help with the
AUC experiments. We acknowledge CF BIC of CIISB, Instruct-CZ Centre, supported by
MEYS CR (LM2023042)) and European Regional Development Fund-Project, UP CIISB“ (No.
CZ.02.1.01/0.0/0.0/18_046/0015974). We thank the members of the Karagöz lab for
the critical reading and editing of the manuscript. We are thankful to our colleagues
Diego Acosta-Alvear, Vladislav Belyy, Jirka Peschek, Yasin Dagdas, Javier Martinez,
Sascha Martens and Alwin Köhler for their invaluable input on the manuscript. We
are grateful to Life Science Editors, especially Katrina Woolcock for her useful
edits and comments on the manuscript. We acknowledge funding from Austrian Science
Fund (FWF-SFB F79 and FWF-W 1261) to GEK. PK acknowledges the support of the Max
Perutz PhD fellowship. GAV is funded by Stand-Alone grants (P30231-B, P30415-B,
P36572), Special Research Grant (SFB grant F79), and Doctoral School grant (DK grant
W1261) from the Austrian Science Fund (FWF). ES and RC acknowledge support and funding
by the Frankfurt Institute of Advanced Studies, the LOEWE Center for Multiscale
Modelling in Life Sciences of the state of Hesse, the Collaborative Research Center
1507 “Membrane-associated Protein Assemblies, Machineries, and Supercomplexes” (Project
ID 450648163), and the International Max Planck Research School on Cellular Biophysics
(to RC), the Center for Scientific Computing of the Goethe University and the Jülich
Supercomputing Centre for computational resources and support.
article_processing_charge: Yes
article_type: original
author:
- first_name: Paulina
full_name: Kettel, Paulina
last_name: Kettel
- first_name: Laura
full_name: Marosits, Laura
last_name: Marosits
- first_name: Elena
full_name: Spinetti, Elena
last_name: Spinetti
- first_name: Michael
full_name: Rechberger, Michael
last_name: Rechberger
- first_name: Caterina
full_name: Giannini, Caterina
id: e3fdddd5-f6e0-11ea-865d-ca99ee6367f4
last_name: Giannini
- first_name: Philipp
full_name: Radler, Philipp
id: 40136C2A-F248-11E8-B48F-1D18A9856A87
last_name: Radler
orcid: '0000-0001-9198-2182 '
- first_name: Isabell
full_name: Niedermoser, Isabell
last_name: Niedermoser
- first_name: Irmgard
full_name: Fischer, Irmgard
last_name: Fischer
- first_name: Gijs A.
full_name: Versteeg, Gijs A.
last_name: Versteeg
- first_name: Martin
full_name: Loose, Martin
id: 462D4284-F248-11E8-B48F-1D18A9856A87
last_name: Loose
orcid: 0000-0001-7309-9724
- first_name: Roberto
full_name: Covino, Roberto
last_name: Covino
- first_name: G. Elif
full_name: Karagöz, G. Elif
last_name: Karagöz
citation:
ama: Kettel P, Marosits L, Spinetti E, et al. Disordered regions in the IRE1α ER
lumenal domain mediate its stress-induced clustering. EMBO Journal. 2024;43(20):4668-4698.
doi:10.1038/s44318-024-00207-0
apa: Kettel, P., Marosits, L., Spinetti, E., Rechberger, M., Giannini, C., Radler,
P., … Karagöz, G. E. (2024). Disordered regions in the IRE1α ER lumenal domain
mediate its stress-induced clustering. EMBO Journal. Embo Press. https://doi.org/10.1038/s44318-024-00207-0
chicago: Kettel, Paulina, Laura Marosits, Elena Spinetti, Michael Rechberger, Caterina
Giannini, Philipp Radler, Isabell Niedermoser, et al. “Disordered Regions in the
IRE1α ER Lumenal Domain Mediate Its Stress-Induced Clustering.” EMBO Journal.
Embo Press, 2024. https://doi.org/10.1038/s44318-024-00207-0.
ieee: P. Kettel et al., “Disordered regions in the IRE1α ER lumenal domain
mediate its stress-induced clustering,” EMBO Journal, vol. 43, no. 20.
Embo Press, pp. 4668–4698, 2024.
ista: Kettel P, Marosits L, Spinetti E, Rechberger M, Giannini C, Radler P, Niedermoser
I, Fischer I, Versteeg GA, Loose M, Covino R, Karagöz GE. 2024. Disordered regions
in the IRE1α ER lumenal domain mediate its stress-induced clustering. EMBO Journal.
43(20), 4668–4698.
mla: Kettel, Paulina, et al. “Disordered Regions in the IRE1α ER Lumenal Domain
Mediate Its Stress-Induced Clustering.” EMBO Journal, vol. 43, no. 20,
Embo Press, 2024, pp. 4668–98, doi:10.1038/s44318-024-00207-0.
short: P. Kettel, L. Marosits, E. Spinetti, M. Rechberger, C. Giannini, P. Radler,
I. Niedermoser, I. Fischer, G.A. Versteeg, M. Loose, R. Covino, G.E. Karagöz,
EMBO Journal 43 (2024) 4668–4698.
das_tickbox: '1'
dataavailabilitystatement: 'All the raw data in this work has been deposited to Biostudies
with the accession number: S-BIAD1023. One can access the data through the link
below: https://www.ebi.ac.uk/biostudies/bioimages/studies/S-BIAD1023?key=426953ee-3da4-4cf4-a496-e607ce240aff. The
source data of this paper are collected in the following database record: biostudies:S-SCDT-10_1038-S44318-024-00207-0.'
date_created: 2024-09-15T22:01:42Z
date_published: 2024-10-15T00:00:00Z
date_updated: 2026-10-16T10:37:47Z
day: '15'
ddc:
- '570'
department:
- _id: MaLo
- _id: JiFr
doi: 10.1038/s44318-024-00207-0
external_id:
isi:
- '001306286100002'
pmid:
- '39232130'
file:
- access_level: open_access
checksum: 04f4df1a561083f2846676442fc4eb3c
content_type: application/pdf
creator: dernst
date_created: 2025-01-13T08:43:20Z
date_updated: 2025-01-13T08:43:20Z
file_id: '18827'
file_name: 2024_Embo_Kettel.pdf
file_size: 10080854
relation: main_file
success: 1
file_date_updated: 2025-01-13T08:43:20Z
fulldoi: https://doi.org/10.1038/s44318-024-00207-0
has_accepted_license: '1'
intvolume: ' 43'
isi: 1
issue: '20'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 4668-4698
pmid: 1
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
researchdata_availability: yes
scopus_import: '1'
status: public
supplementarymaterial: yes
title: Disordered regions in the IRE1α ER lumenal domain mediate its stress-induced
clustering
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 43
year: '2024'
...
---
_id: '13342'
abstract:
- lang: eng
text: Motile cells moving in multicellular organisms encounter microenvironments
of locally heterogeneous mechanochemical composition. Individual compositional
parameters like chemotactic signals, adhesiveness, and pore sizes are well known
to be sensed by motile cells, providing individual guidance cues for cellular
pathfinding. However, motile cells encounter diverse mechanochemical signals at
the same time, raising the question of how cells respond to locally diverse and
potentially competing signals on their migration routes. Here, we reveal that
motile amoeboid cells require nuclear repositioning, termed nucleokinesis, for
adaptive pathfinding in heterogeneous mechanochemical microenvironments. Using
mammalian immune cells and the amoebaDictyostelium discoideum,
we discover that frequent, rapid and long-distance nucleokinesis is a basic component
of amoeboid pathfinding, enabling cells to reorientate quickly between locally
competing cues. Amoeboid nucleokinesis comprises a two-step cell polarity switch
and is driven by myosin II-forces, sliding the nucleus from a ‘losing’ to the
‘winning’ leading edge to re-adjust the nuclear to the cellular path. Impaired
nucleokinesis distorts fast path adaptions and causes cellular arrest in the microenvironment.
Our findings establish that nucleokinesis is required for amoeboid cell navigation.
Given that motile single-cell amoebae, many immune cells, and some cancer cells
utilize an amoeboid migration strategy, these results suggest that amoeboid nucleokinesis
underlies cellular navigation during unicellular biology, immunity, and disease.
acknowledgement: We thank Christoph Mayr and Bingzhi Wang for initial experiments
on amoeboid nucleokinesis, Ana-Maria Lennon-Duménil and Aline Yatim for bone marrow
from MyoIIA-Flox*CD11c-Cre mice, Michael Sixt and Aglaja Kopf for EMTB-mCherry,
EB3-mCherry, Lifeact-GFP, Lfc knockout, and Myh9-GFP expressing HoxB8 cells, Malte
Benjamin Braun, Mauricio Ruiz, and Madeleine T. Schmitt for critical reading of
the manuscript, and the Core Facility Bioimaging, the Core Facility Flow Cytometry,
and the Animal Core Facility of the Biomedical Center (BMC) for excellent support.
This study was supported by the Peter Hans Hofschneider Professorship of the foundation
“Stiftung Experimentelle Biomedizin” (to JR), the LMU Institutional Strategy LMU-Excellent
within the framework of the German Excellence Initiative (to JR), and the Deutsche
Forschungsgemeinschaft (DFG; German Research Foundation; SFB914 project A12, to
JR), and the CZI grant DAF2020-225401 (https://doi.org/10.37921/120055ratwvi) from
the Chan Zuckerberg Initiative DAF (to RH; an advised fund of Silicon Valley Community
Foundation (funder https://doi.org/10.13039/100014989)). Open Access funding enabled
and organized by Projekt DEAL.
article_number: e114557
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Janina
full_name: Kroll, Janina
last_name: Kroll
- first_name: Robert
full_name: Hauschild, Robert
id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
last_name: Hauschild
orcid: 0000-0001-9843-3522
- first_name: Arthur
full_name: Kuznetcov, Arthur
last_name: Kuznetcov
- first_name: Kasia
full_name: Stefanowski, Kasia
last_name: Stefanowski
- first_name: Monika D.
full_name: Hermann, Monika D.
last_name: Hermann
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Lubuna B
full_name: Shafeek, Lubuna B
id: 3CD37A82-F248-11E8-B48F-1D18A9856A87
last_name: Shafeek
orcid: 0000-0001-7180-6050
- first_name: Annette
full_name: Müller-Taubenberger, Annette
last_name: Müller-Taubenberger
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
citation:
ama: Kroll J, Hauschild R, Kuznetcov A, et al. Adaptive pathfinding by nucleokinesis
during amoeboid migration. EMBO Journal. 2023. doi:10.15252/embj.2023114557
apa: Kroll, J., Hauschild, R., Kuznetcov, A., Stefanowski, K., Hermann, M. D., Merrin,
J., … Renkawitz, J. (2023). Adaptive pathfinding by nucleokinesis during amoeboid
migration. EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2023114557
chicago: Kroll, Janina, Robert Hauschild, Arthur Kuznetcov, Kasia Stefanowski, Monika
D. Hermann, Jack Merrin, Lubuna B Shafeek, Annette Müller-Taubenberger, and Jörg
Renkawitz. “Adaptive Pathfinding by Nucleokinesis during Amoeboid Migration.”
EMBO Journal. Embo Press, 2023. https://doi.org/10.15252/embj.2023114557.
ieee: J. Kroll et al., “Adaptive pathfinding by nucleokinesis during amoeboid
migration,” EMBO Journal. Embo Press, 2023.
ista: Kroll J, Hauschild R, Kuznetcov A, Stefanowski K, Hermann MD, Merrin J, Shafeek
LB, Müller-Taubenberger A, Renkawitz J. 2023. Adaptive pathfinding by nucleokinesis
during amoeboid migration. EMBO Journal., e114557.
mla: Kroll, Janina, et al. “Adaptive Pathfinding by Nucleokinesis during Amoeboid
Migration.” EMBO Journal, e114557, Embo Press, 2023, doi:10.15252/embj.2023114557.
short: J. Kroll, R. Hauschild, A. Kuznetcov, K. Stefanowski, M.D. Hermann, J. Merrin,
L.B. Shafeek, A. Müller-Taubenberger, J. Renkawitz, EMBO Journal (2023).
date_created: 2023-08-01T08:59:06Z
date_published: 2023-11-21T00:00:00Z
date_updated: 2025-09-09T12:44:04Z
day: '21'
ddc:
- '570'
department:
- _id: NanoFab
- _id: Bio
doi: 10.15252/embj.2023114557
external_id:
isi:
- '001120971800001'
pmid:
- '37987147'
file:
- access_level: open_access
checksum: 6261d0041c7e8d284c39712c40079730
content_type: application/pdf
creator: dernst
date_created: 2023-11-27T08:45:56Z
date_updated: 2023-11-27T08:45:56Z
file_id: '14611'
file_name: 2023_EmboJournal_Kroll.pdf
file_size: 4862497
relation: main_file
success: 1
file_date_updated: 2023-11-27T08:45:56Z
fulldoi: https://doi.org/10.15252/embj.2023114557
has_accepted_license: '1'
isi: 1
language:
- iso: eng
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Adaptive pathfinding by nucleokinesis during amoeboid migration
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
year: '2023'
...
---
OA_place: publisher
OA_type: gold
_id: '23131'
abstract:
- lang: eng
text: Roots are highly plastic organs enabling plants to adapt to a changing below‐ground
environment. In addition to abiotic factors like nutrients or mechanical resistance,
plant roots also respond to temperature variation. Below the heat stress threshold,
Arabidopsis thaliana seedlings react to elevated temperature by promoting primary
root growth, possibly to reach deeper soil regions with potentially better water
saturation. While above‐ground thermomorphogenesis is enabled by thermo‐sensitive
cell elongation, it was unknown how temperature modulates root growth. We here
show that roots are able to sense and respond to elevated temperature independently
of shoot‐derived signals. This response is mediated by a yet unknown root thermosensor
that employs auxin as a messenger to relay temperature signals to the cell cycle.
Growth promotion is achieved primarily by increasing cell division rates in the
root apical meristem, depending on de novo local auxin biosynthesis and temperature‐sensitive
organization of the polar auxin transport system. Hence, the primary cellular
target of elevated ambient temperature differs fundamentally between root and
shoot tissues, while the messenger auxin remains the same.
article_number: EMBJ2022111926
article_processing_charge: Yes
article_type: original
author:
- first_name: Haiyue
full_name: Ai, Haiyue
last_name: Ai
- first_name: Julia
full_name: Bellstaedt, Julia
last_name: Bellstaedt
- first_name: Kai
full_name: Bartusch, Kai
id: 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
last_name: Bartusch
orcid: 0000-0003-2708-5545
- first_name: Lennart
full_name: Eschen‐Lippold, Lennart
last_name: Eschen‐Lippold
- first_name: Steve
full_name: Babben, Steve
last_name: Babben
- first_name: Gerd Ulrich
full_name: Balcke, Gerd Ulrich
last_name: Balcke
- first_name: Alain
full_name: Tissier, Alain
last_name: Tissier
- first_name: Bettina
full_name: Hause, Bettina
last_name: Hause
- first_name: Tonni Grube
full_name: Andersen, Tonni Grube
last_name: Andersen
- first_name: Carolin
full_name: Delker, Carolin
last_name: Delker
- first_name: Marcel
full_name: Quint, Marcel
last_name: Quint
citation:
ama: Ai H, Bellstaedt J, Bartusch K, et al. Auxin‐dependent regulation of cell division
rates governs root thermomorphogenesis. The EMBO Journal. 2023;42(11).
doi:10.15252/embj.2022111926
apa: Ai, H., Bellstaedt, J., Bartusch, K., Eschen‐Lippold, L., Babben, S., Balcke,
G. U., … Quint, M. (2023). Auxin‐dependent regulation of cell division rates governs
root thermomorphogenesis. The EMBO Journal. Springer. https://doi.org/10.15252/embj.2022111926
chicago: Ai, Haiyue, Julia Bellstaedt, Kai Bartusch, Lennart Eschen‐Lippold, Steve
Babben, Gerd Ulrich Balcke, Alain Tissier, et al. “Auxin‐dependent Regulation
of Cell Division Rates Governs Root Thermomorphogenesis.” The EMBO Journal.
Springer, 2023. https://doi.org/10.15252/embj.2022111926.
ieee: H. Ai et al., “Auxin‐dependent regulation of cell division rates governs
root thermomorphogenesis,” The EMBO Journal, vol. 42, no. 11. Springer,
2023.
ista: Ai H, Bellstaedt J, Bartusch K, Eschen‐Lippold L, Babben S, Balcke GU, Tissier
A, Hause B, Andersen TG, Delker C, Quint M. 2023. Auxin‐dependent regulation of
cell division rates governs root thermomorphogenesis. The EMBO Journal. 42(11),
EMBJ2022111926.
mla: Ai, Haiyue, et al. “Auxin‐dependent Regulation of Cell Division Rates Governs
Root Thermomorphogenesis.” The EMBO Journal, vol. 42, no. 11, EMBJ2022111926,
Springer, 2023, doi:10.15252/embj.2022111926.
short: H. Ai, J. Bellstaedt, K. Bartusch, L. Eschen‐Lippold, S. Babben, G.U. Balcke,
A. Tissier, B. Hause, T.G. Andersen, C. Delker, M. Quint, The EMBO Journal 42
(2023).
date_created: 2026-10-13T11:15:14Z
date_published: 2023-04-18T00:00:00Z
date_updated: 2026-10-14T10:36:57Z
day: '18'
ddc:
- '580'
doi: 10.15252/embj.2022111926
extern: '1'
external_id:
pmid:
- '37071525'
fulldoi: https://doi.org/10.15252/embj.2022111926
has_accepted_license: '1'
intvolume: ' 42'
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.15252/embj.2022111926
month: '04'
oa: 1
oa_version: Published Version
pmid: 1
publication: The EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: '1'
status: public
title: Auxin‐dependent regulation of cell division rates governs root thermomorphogenesis
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 42
year: '2023'
...
---
_id: '15138'
abstract:
- lang: eng
text: RNA viruses induce the formation of subcellular organelles that provide microenvironments
conducive to their replication. Here we show that replication factories of rotaviruses
represent protein‐RNA condensates that are formed via liquid–liquid phase separation
of the viroplasm‐forming proteins NSP5 and rotavirus RNA chaperone NSP2. Upon
mixing, these proteins readily form condensates at physiologically relevant low
micromolar concentrations achieved in the cytoplasm of virus‐infected cells. Early
infection stage condensates could be reversibly dissolved by 1,6‐hexanediol, as
well as propylene glycol that released rotavirus transcripts from these condensates.
During the early stages of infection, propylene glycol treatments reduced viral
replication and phosphorylation of the condensate‐forming protein NSP5. During
late infection, these condensates exhibited altered material properties and became
resistant to propylene glycol, coinciding with hyperphosphorylation of NSP5. Some
aspects of the assembly of cytoplasmic rotavirus replication factories mirror
the formation of other ribonucleoprotein granules. Such viral RNA‐rich condensates
that support replication of multi‐segmented genomes represent an attractive target
for developing novel therapeutic approaches.
article_number: e107711
article_processing_charge: Yes
article_type: original
author:
- first_name: Florian
full_name: Geiger, Florian
last_name: Geiger
- first_name: Julia
full_name: Acker, Julia
last_name: Acker
- first_name: Guido
full_name: Papa, Guido
last_name: Papa
- first_name: Xinyu
full_name: Wang, Xinyu
last_name: Wang
- first_name: William E
full_name: Arter, William E
last_name: Arter
- first_name: Kadi L
full_name: Saar, Kadi L
last_name: Saar
- first_name: Nadia A
full_name: Erkamp, Nadia A
last_name: Erkamp
- first_name: Runzhang
full_name: Qi, Runzhang
last_name: Qi
- first_name: Jack Peter Kelly
full_name: Bravo, Jack Peter Kelly
id: 96aecfa5-8931-11ee-af30-aa6a5d6eee0e
last_name: Bravo
orcid: 0000-0003-0456-0753
- first_name: Sebastian
full_name: Strauss, Sebastian
last_name: Strauss
- first_name: Georg
full_name: Krainer, Georg
last_name: Krainer
- first_name: Oscar R
full_name: Burrone, Oscar R
last_name: Burrone
- first_name: Ralf
full_name: Jungmann, Ralf
last_name: Jungmann
- first_name: Tuomas PJ
full_name: Knowles, Tuomas PJ
last_name: Knowles
- first_name: Hanna
full_name: Engelke, Hanna
last_name: Engelke
- first_name: Alexander
full_name: Borodavka, Alexander
last_name: Borodavka
citation:
ama: Geiger F, Acker J, Papa G, et al. Liquid–liquid phase separation underpins
the formation of replication factories in rotaviruses. The EMBO Journal.
2021;40(21). doi:10.15252/embj.2021107711
apa: Geiger, F., Acker, J., Papa, G., Wang, X., Arter, W. E., Saar, K. L., … Borodavka,
A. (2021). Liquid–liquid phase separation underpins the formation of replication
factories in rotaviruses. The EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2021107711
chicago: Geiger, Florian, Julia Acker, Guido Papa, Xinyu Wang, William E Arter,
Kadi L Saar, Nadia A Erkamp, et al. “Liquid–Liquid Phase Separation Underpins
the Formation of Replication Factories in Rotaviruses.” The EMBO Journal.
Embo Press, 2021. https://doi.org/10.15252/embj.2021107711.
ieee: F. Geiger et al., “Liquid–liquid phase separation underpins the formation
of replication factories in rotaviruses,” The EMBO Journal, vol. 40, no.
21. Embo Press, 2021.
ista: Geiger F, Acker J, Papa G, Wang X, Arter WE, Saar KL, Erkamp NA, Qi R, Bravo
JPK, Strauss S, Krainer G, Burrone OR, Jungmann R, Knowles TP, Engelke H, Borodavka
A. 2021. Liquid–liquid phase separation underpins the formation of replication
factories in rotaviruses. The EMBO Journal. 40(21), e107711.
mla: Geiger, Florian, et al. “Liquid–Liquid Phase Separation Underpins the Formation
of Replication Factories in Rotaviruses.” The EMBO Journal, vol. 40, no.
21, e107711, Embo Press, 2021, doi:10.15252/embj.2021107711.
short: F. Geiger, J. Acker, G. Papa, X. Wang, W.E. Arter, K.L. Saar, N.A. Erkamp,
R. Qi, J.P.K. Bravo, S. Strauss, G. Krainer, O.R. Burrone, R. Jungmann, T.P. Knowles,
H. Engelke, A. Borodavka, The EMBO Journal 40 (2021).
date_created: 2024-03-20T10:42:39Z
date_published: 2021-11-02T00:00:00Z
date_updated: 2024-06-04T06:08:16Z
day: '02'
doi: 10.15252/embj.2021107711
extern: '1'
external_id:
pmid:
- '34524703'
fulldoi: https://doi.org/10.15252/embj.2021107711
intvolume: ' 40'
issue: '21'
keyword:
- General Immunology and Microbiology
- General Biochemistry
- Genetics and Molecular Biology
- Molecular Biology
- General Neuroscience
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.15252/embj.2021107711
month: '11'
oa: 1
oa_version: Published Version
pmid: 1
publication: The EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Liquid–liquid phase separation underpins the formation of replication factories
in rotaviruses
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 40
year: '2021'
...
---
_id: '10179'
abstract:
- lang: eng
text: Inhibitory GABAergic interneurons migrate over long distances from their extracortical
origin into the developing cortex. In humans, this process is uniquely slow and
prolonged, and it is unclear whether guidance cues unique to humans govern the
various phases of this complex developmental process. Here, we use fused cerebral
organoids to identify key roles of neurotransmitter signaling pathways in guiding
the migratory behavior of human cortical interneurons. We use scRNAseq to reveal
expression of GABA, glutamate, glycine, and serotonin receptors along distinct
maturation trajectories across interneuron migration. We develop an image analysis
software package, TrackPal, to simultaneously assess 48 parameters for entire
migration tracks of individual cells. By chemical screening, we show that different
modes of interneuron migration depend on distinct neurotransmitter signaling pathways,
linking transcriptional maturation of interneurons with their migratory behavior.
Altogether, our study provides a comprehensive quantitative analysis of human
interneuron migration and its functional modulation by neurotransmitter signaling.
acknowledgement: We thank all Knoblich laboratory members for continued support and
discussions. We thank the IMP/IMBA BioOptics facility, particularly Pawel Pasierbek,
Alberto Moreno Cencerrado and Gerald Schmauss, the IMP/IMBA Molecular Biology Service,
in particular Robert Heinen, the IMP Bioinformatics facility, in particular Thomas
Burkard, the Vienna Biocenter Core Facilities (VBCF) Histopathology facility, in
particular Tamara Engelmaier, and the VBCF Next Generation Sequencing Facility,
notably Volodymyr Shubchynskyy and Carmen Czepe. We would also like to thank Simon
Haendeler for advice on statistical analyses, Jose Guzman for discussions and assistance
with slice culture setups, Oliver L. Eichmueller for discussions and assistance
with microscopy, and E.H. Gustafson, S. Wolfinger, and D. Reumann for technical
assistance regarding generation of cerebral organoids. This project received funding
from the European Union’s Horizon 2020 research and innovation program under the
Marie Skłodowska-Curie fellowship agreement Nr.707109 awarded to J.A.B. Work in
J.A.K.'s laboratory is supported by the Austrian Federal Ministry of Education,
Science and Research, the Austrian Academy of Sciences, the City of Vienna, a Research
Program of the Austrian Science Fund FWF (SFBF78 Stem Cell, F 7803-B) and a European
Research Council (ERC) Advanced Grant under the European 20 Union’s Horizon 2020
program (grant agreement no. 695642).
article_number: e108714
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Sunanjay
full_name: Bajaj, Sunanjay
last_name: Bajaj
- first_name: Joshua A.
full_name: Bagley, Joshua A.
last_name: Bagley
- first_name: Christoph M
full_name: Sommer, Christoph M
id: 4DF26D8C-F248-11E8-B48F-1D18A9856A87
last_name: Sommer
orcid: 0000-0003-1216-9105
- first_name: Abel
full_name: Vertesy, Abel
last_name: Vertesy
- first_name: Sakurako
full_name: Nagumo Wong, Sakurako
last_name: Nagumo Wong
- first_name: Veronica
full_name: Krenn, Veronica
last_name: Krenn
- first_name: Julie
full_name: Lévi-Strauss, Julie
last_name: Lévi-Strauss
- first_name: Juergen A.
full_name: Knoblich, Juergen A.
last_name: Knoblich
citation:
ama: Bajaj S, Bagley JA, Sommer CM, et al. Neurotransmitter signaling regulates
distinct phases of multimodal human interneuron migration. EMBO Journal.
2021;40(23). doi:10.15252/embj.2021108714
apa: Bajaj, S., Bagley, J. A., Sommer, C. M., Vertesy, A., Nagumo Wong, S., Krenn,
V., … Knoblich, J. A. (2021). Neurotransmitter signaling regulates distinct phases
of multimodal human interneuron migration. EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2021108714
chicago: Bajaj, Sunanjay, Joshua A. Bagley, Christoph M Sommer, Abel Vertesy, Sakurako
Nagumo Wong, Veronica Krenn, Julie Lévi-Strauss, and Juergen A. Knoblich. “Neurotransmitter
Signaling Regulates Distinct Phases of Multimodal Human Interneuron Migration.”
EMBO Journal. Embo Press, 2021. https://doi.org/10.15252/embj.2021108714.
ieee: S. Bajaj et al., “Neurotransmitter signaling regulates distinct phases
of multimodal human interneuron migration,” EMBO Journal, vol. 40, no.
23. Embo Press, 2021.
ista: Bajaj S, Bagley JA, Sommer CM, Vertesy A, Nagumo Wong S, Krenn V, Lévi-Strauss
J, Knoblich JA. 2021. Neurotransmitter signaling regulates distinct phases of
multimodal human interneuron migration. EMBO Journal. 40(23), e108714.
mla: Bajaj, Sunanjay, et al. “Neurotransmitter Signaling Regulates Distinct Phases
of Multimodal Human Interneuron Migration.” EMBO Journal, vol. 40, no.
23, e108714, Embo Press, 2021, doi:10.15252/embj.2021108714.
short: S. Bajaj, J.A. Bagley, C.M. Sommer, A. Vertesy, S. Nagumo Wong, V. Krenn,
J. Lévi-Strauss, J.A. Knoblich, EMBO Journal 40 (2021).
date_created: 2021-10-24T22:01:34Z
date_published: 2021-10-18T00:00:00Z
date_updated: 2023-08-14T08:05:23Z
day: '18'
ddc:
- '610'
department:
- _id: Bio
doi: 10.15252/embj.2021108714
external_id:
isi:
- '000708012800001'
pmid:
- '34661293'
file:
- access_level: open_access
checksum: 78d2d02e775322297e774f72810a41a4
content_type: application/pdf
creator: alisjak
date_created: 2021-12-13T14:54:14Z
date_updated: 2021-12-13T14:54:14Z
file_id: '10541'
file_name: 2021_EMBO_Bajaj.pdf
file_size: 7819881
relation: main_file
success: 1
file_date_updated: 2021-12-13T14:54:14Z
fulldoi: https://doi.org/10.15252/embj.2021108714
has_accepted_license: '1'
intvolume: ' 40'
isi: 1
issue: '23'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Neurotransmitter signaling regulates distinct phases of multimodal human interneuron
migration
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 40
year: '2021'
...
---
_id: '9010'
abstract:
- lang: eng
text: Availability of the essential macronutrient nitrogen in soil plays a critical
role in plant growth, development, and impacts agricultural productivity. Plants
have evolved different strategies for sensing and responding to heterogeneous
nitrogen distribution. Modulation of root system architecture, including primary
root growth and branching, is among the most essential plant adaptions to ensure
adequate nitrogen acquisition. However, the immediate molecular pathways coordinating
the adjustment of root growth in response to distinct nitrogen sources, such as
nitrate or ammonium, are poorly understood. Here, we show that growth as manifested
by cell division and elongation is synchronized by coordinated auxin flux between
two adjacent outer tissue layers of the root. This coordination is achieved by
nitrate‐dependent dephosphorylation of the PIN2 auxin efflux carrier at a previously
uncharacterized phosphorylation site, leading to subsequent PIN2 lateralization
and thereby regulating auxin flow between adjacent tissues. A dynamic computer
model based on our experimental data successfully recapitulates experimental observations.
Our study provides mechanistic insights broadening our understanding of root growth
mechanisms in dynamic environments.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We acknowledge Gergely Molnar for critical reading of the manuscript,
Alexander Johnson for language editing and Yulija Salanenka for technical assistance.
Work in the Benkova laboratory was supported by the Austrian Science Fund (FWF01_I1774S)
to KO, RA and EB. Work in the Benkova laboratory was supported by the Austrian Science
Fund (FWF01_I1774S) to KO, RA and EB and by the DOC Fellowship Programme of the
AustrianAcademy of Sciences (25008) to C.A. Work in the Wabnik laboratory was supported
by the Programa de Atraccion de Talento 2017 (Comunidad deMadrid, 2017-T1/BIO-5654
to K.W.), Severo Ochoa Programme for Centres of Excellence in R&D from the Agencia
Estatal de Investigacion of Spain (grantSEV-2016-0672 (2017-2021) to K.W. via the
CBGP) and Programa Estatal de Generacion del Conocimiento y Fortalecimiento Científico
y Tecnologico del Sistema de I+D+I 2019 (PGC2018-093387-A-I00) from MICIU (to K.W.).
M.M.was supported by a postdoctoral contract associated to SEV-2016-0672.We acknowledge
the Bioimaging Facility in IST-Austria and the Advanced Microscopy Facility of the
Vienna Bio Center Core Facilities, member of the Vienna Bio Center Austria, for
use of the OMX v43D SIM microscope. AJ was supported by the Austrian Science Fund
(FWF): I03630 to J.F'
article_number: e106862
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: Marco
full_name: Marconi, Marco
last_name: Marconi
- first_name: Andrea
full_name: Vega, Andrea
last_name: Vega
- first_name: Jose
full_name: O’Brien, Jose
last_name: O’Brien
- first_name: Alexander J
full_name: Johnson, Alexander J
id: 46A62C3A-F248-11E8-B48F-1D18A9856A87
last_name: Johnson
orcid: 0000-0002-2739-8843
- first_name: Rashed
full_name: Abualia, Rashed
id: 4827E134-F248-11E8-B48F-1D18A9856A87
last_name: Abualia
orcid: 0000-0002-9357-9415
- first_name: Livio
full_name: Antonielli, Livio
last_name: Antonielli
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: Yuzhou
full_name: Zhang, Yuzhou
id: 3B6137F2-F248-11E8-B48F-1D18A9856A87
last_name: Zhang
orcid: 0000-0003-2627-6956
- first_name: Shutang
full_name: Tan, Shutang
id: 2DE75584-F248-11E8-B48F-1D18A9856A87
last_name: Tan
orcid: 0000-0002-0471-8285
- first_name: Candela
full_name: Cuesta, Candela
id: 33A3C818-F248-11E8-B48F-1D18A9856A87
last_name: Cuesta
orcid: 0000-0003-1923-2410
- first_name: Christina
full_name: Artner, Christina
id: 45DF286A-F248-11E8-B48F-1D18A9856A87
last_name: Artner
- first_name: Eleonore
full_name: Bouguyon, Eleonore
last_name: Bouguyon
- first_name: Alain
full_name: Gojon, Alain
last_name: Gojon
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Rodrigo A.
full_name: Gutiérrez, Rodrigo A.
last_name: Gutiérrez
- first_name: Krzysztof T
full_name: Wabnik, Krzysztof T
id: 4DE369A4-F248-11E8-B48F-1D18A9856A87
last_name: Wabnik
orcid: 0000-0001-7263-0560
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Ötvös K, Marconi M, Vega A, et al. Modulation of plant root growth by nitrogen
source-defined regulation of polar auxin transport. EMBO Journal. 2021;40(3).
doi:10.15252/embj.2020106862
apa: Ötvös, K., Marconi, M., Vega, A., O’Brien, J., Johnson, A. J., Abualia, R.,
… Benková, E. (2021). Modulation of plant root growth by nitrogen source-defined
regulation of polar auxin transport. EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2020106862
chicago: Ötvös, Krisztina, Marco Marconi, Andrea Vega, Jose O’Brien, Alexander J
Johnson, Rashed Abualia, Livio Antonielli, et al. “Modulation of Plant Root Growth
by Nitrogen Source-Defined Regulation of Polar Auxin Transport.” EMBO Journal.
Embo Press, 2021. https://doi.org/10.15252/embj.2020106862.
ieee: K. Ötvös et al., “Modulation of plant root growth by nitrogen source-defined
regulation of polar auxin transport,” EMBO Journal, vol. 40, no. 3. Embo
Press, 2021.
ista: Ötvös K, Marconi M, Vega A, O’Brien J, Johnson AJ, Abualia R, Antonielli L,
Montesinos López JC, Zhang Y, Tan S, Cuesta C, Artner C, Bouguyon E, Gojon A,
Friml J, Gutiérrez RA, Wabnik KT, Benková E. 2021. Modulation of plant root growth
by nitrogen source-defined regulation of polar auxin transport. EMBO Journal.
40(3), e106862.
mla: Ötvös, Krisztina, et al. “Modulation of Plant Root Growth by Nitrogen Source-Defined
Regulation of Polar Auxin Transport.” EMBO Journal, vol. 40, no. 3, e106862,
Embo Press, 2021, doi:10.15252/embj.2020106862.
short: K. Ötvös, M. Marconi, A. Vega, J. O’Brien, A.J. Johnson, R. Abualia, L. Antonielli,
J.C. Montesinos López, Y. Zhang, S. Tan, C. Cuesta, C. Artner, E. Bouguyon, A.
Gojon, J. Friml, R.A. Gutiérrez, K.T. Wabnik, E. Benková, EMBO Journal 40 (2021).
corr_author: '1'
date_created: 2021-01-17T23:01:12Z
date_published: 2021-02-01T00:00:00Z
date_updated: 2026-11-05T23:30:41Z
day: '01'
ddc:
- '580'
department:
- _id: JiFr
- _id: EvBe
doi: 10.15252/embj.2020106862
external_id:
isi:
- '000604645600001'
pmid:
- ' 33399250'
file:
- access_level: open_access
checksum: dc55c900f3b061d6c2790b8813d759a3
content_type: application/pdf
creator: dernst
date_created: 2021-02-11T12:28:29Z
date_updated: 2021-02-11T12:28:29Z
file_id: '9110'
file_name: 2021_Embo_Otvos.pdf
file_size: 2358617
relation: main_file
success: 1
file_date_updated: 2021-02-11T12:28:29Z
fulldoi: https://doi.org/10.15252/embj.2020106862
has_accepted_license: '1'
intvolume: ' 40'
isi: 1
issue: '3'
language:
- iso: eng
month: '02'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
- _id: 2685A872-B435-11E9-9278-68D0E5697425
name: Hormonal regulation of plant adaptive responses to environmental signals
- _id: 26538374-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I03630
name: Molecular mechanisms of endocytic cargo recognition in plants
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
related_material:
link:
- description: News on IST Homepage
relation: press_release
url: https://ist.ac.at/en/news/a-plants-way-to-its-favorite-food/
record:
- id: '10303'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Modulation of plant root growth by nitrogen source-defined regulation of polar
auxin transport
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 40
year: '2021'
...
---
_id: '7586'
abstract:
- lang: eng
text: CLC chloride/proton exchangers may support acidification of endolysosomes
and raise their luminal Cl− concentration. Disruption of endosomal ClC‐3 causes
severe neurodegeneration. To assess the importance of ClC‐3 Cl−/H+ exchange, we
now generate Clcn3unc/unc mice in which ClC‐3 is converted into a Cl− channel.
Unlike Clcn3−/− mice, Clcn3unc/unc mice appear normal owing to compensation by
ClC‐4 with which ClC‐3 forms heteromers. ClC‐4 protein levels are strongly reduced
in Clcn3−/−, but not in Clcn3unc/unc mice because ClC‐3unc binds and stabilizes
ClC‐4 like wild‐type ClC‐3. Although mice lacking ClC‐4 appear healthy, its absence
in Clcn3unc/unc/Clcn4−/− mice entails even stronger neurodegeneration than observed
in Clcn3−/− mice. A fraction of ClC‐3 is found on synaptic vesicles, but miniature
postsynaptic currents and synaptic vesicle acidification are not affected in Clcn3unc/unc
or Clcn3−/− mice before neurodegeneration sets in. Both, Cl−/H+‐exchange activity
and the stabilizing effect on ClC‐4, are central to the biological function of
ClC‐3.
acknowledgement: "We thank T. Stauber and T. Breiderhoff for cloning expression constructs;
K. Räbel, S. Hohensee, and C. Backhaus for technical assistance; R. Jahn (MPIbpc,
Göttingen) for providing the equipment required for SV purification; and A\r\nWoehler
(MDC, Berlin) for assistance with SV imaging. Supported, in part, by grants from
the Deutsche Forschungsgemeinschaft (JE164/9-2, SFB740 TP C5, FOR 2625 (JE164/14-1),
NeuroCure Cluster of Excellence), the European Research Council Advanced Grant CYTOVOLION
(ERC 294435) and the Prix Louis-Jeantet de Médecine to TJJ, and Peter and Traudl
Engelhorn fellowship to ZF."
article_number: e103358
article_processing_charge: No
article_type: original
author:
- first_name: Stefanie
full_name: Weinert, Stefanie
last_name: Weinert
- first_name: Niclas
full_name: Gimber, Niclas
last_name: Gimber
- first_name: Dorothea
full_name: Deuschel, Dorothea
last_name: Deuschel
- first_name: Till
full_name: Stuhlmann, Till
last_name: Stuhlmann
- first_name: Dmytro
full_name: Puchkov, Dmytro
last_name: Puchkov
- first_name: Zohreh
full_name: Farsi, Zohreh
last_name: Farsi
- first_name: Carmen F.
full_name: Ludwig, Carmen F.
last_name: Ludwig
- first_name: Gaia
full_name: Novarino, Gaia
id: 3E57A680-F248-11E8-B48F-1D18A9856A87
last_name: Novarino
orcid: 0000-0002-7673-7178
- first_name: Karen I.
full_name: López-Cayuqueo, Karen I.
last_name: López-Cayuqueo
- first_name: Rosa
full_name: Planells-Cases, Rosa
last_name: Planells-Cases
- first_name: Thomas J.
full_name: Jentsch, Thomas J.
last_name: Jentsch
citation:
ama: Weinert S, Gimber N, Deuschel D, et al. Uncoupling endosomal CLC chloride/proton
exchange causes severe neurodegeneration. EMBO Journal. 2020;39. doi:10.15252/embj.2019103358
apa: Weinert, S., Gimber, N., Deuschel, D., Stuhlmann, T., Puchkov, D., Farsi, Z.,
… Jentsch, T. J. (2020). Uncoupling endosomal CLC chloride/proton exchange causes
severe neurodegeneration. EMBO Journal. EMBO Press. https://doi.org/10.15252/embj.2019103358
chicago: Weinert, Stefanie, Niclas Gimber, Dorothea Deuschel, Till Stuhlmann, Dmytro
Puchkov, Zohreh Farsi, Carmen F. Ludwig, et al. “Uncoupling Endosomal CLC Chloride/Proton
Exchange Causes Severe Neurodegeneration.” EMBO Journal. EMBO Press, 2020.
https://doi.org/10.15252/embj.2019103358.
ieee: S. Weinert et al., “Uncoupling endosomal CLC chloride/proton exchange
causes severe neurodegeneration,” EMBO Journal, vol. 39. EMBO Press, 2020.
ista: Weinert S, Gimber N, Deuschel D, Stuhlmann T, Puchkov D, Farsi Z, Ludwig CF,
Novarino G, López-Cayuqueo KI, Planells-Cases R, Jentsch TJ. 2020. Uncoupling
endosomal CLC chloride/proton exchange causes severe neurodegeneration. EMBO Journal.
39, e103358.
mla: Weinert, Stefanie, et al. “Uncoupling Endosomal CLC Chloride/Proton Exchange
Causes Severe Neurodegeneration.” EMBO Journal, vol. 39, e103358, EMBO
Press, 2020, doi:10.15252/embj.2019103358.
short: S. Weinert, N. Gimber, D. Deuschel, T. Stuhlmann, D. Puchkov, Z. Farsi, C.F.
Ludwig, G. Novarino, K.I. López-Cayuqueo, R. Planells-Cases, T.J. Jentsch, EMBO
Journal 39 (2020).
date_created: 2020-03-15T23:00:55Z
date_published: 2020-03-02T00:00:00Z
date_updated: 2026-04-16T09:35:48Z
day: '02'
ddc:
- '570'
department:
- _id: GaNo
doi: 10.15252/embj.2019103358
external_id:
isi:
- '000517335000001'
pmid:
- '32118314'
file:
- access_level: open_access
checksum: 82750a7a93e3740decbce8474004111a
content_type: application/pdf
creator: dernst
date_created: 2020-03-23T13:51:11Z
date_updated: 2020-07-14T12:48:00Z
file_id: '7615'
file_name: 2020_EMBO_Weinert.pdf
file_size: 12243278
relation: main_file
file_date_updated: 2020-07-14T12:48:00Z
fulldoi: https://doi.org/10.15252/embj.2019103358
has_accepted_license: '1'
intvolume: ' 39'
isi: 1
language:
- iso: eng
month: '03'
oa: 1
oa_version: Published Version
pmid: 1
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: EMBO Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 39
year: '2020'
...
---
_id: '8142'
abstract:
- lang: eng
text: Cell production and differentiation for the acquisition of specific functions
are key features of living systems. The dynamic network of cellular microtubules
provides the necessary platform to accommodate processes associated with the transition
of cells through the individual phases of cytogenesis. Here, we show that the
plant hormone cytokinin fine‐tunes the activity of the microtubular cytoskeleton
during cell differentiation and counteracts microtubular rearrangements driven
by the hormone auxin. The endogenous upward gradient of cytokinin activity along
the longitudinal growth axis in Arabidopsis thaliana roots correlates with robust
rearrangements of the microtubule cytoskeleton in epidermal cells progressing
from the proliferative to the differentiation stage. Controlled increases in cytokinin
activity result in premature re‐organization of the microtubule network from transversal
to an oblique disposition in cells prior to their differentiation, whereas attenuated
hormone perception delays cytoskeleton conversion into a configuration typical
for differentiated cells. Intriguingly, cytokinin can interfere with microtubules
also in animal cells, such as leukocytes, suggesting that a cytokinin‐sensitive
control pathway for the microtubular cytoskeleton may be at least partially conserved
between plant and animal cells.
acknowledged_ssus:
- _id: Bio
- _id: LifeSc
acknowledgement: We thank Takashi Aoyama, David Alabadi, and Bert De Rybel for sharing
material, Jiří Friml, Maciek Adamowski, and Katerina Schwarzerová for inspiring
discussions, and Martine De Cock for help in preparing the manuscript. This research
was supported by the Scientific Service Units (SSUs) of IST Austria through resources
provided by the Bioimaging Facility (BIF), especially to Robert Hauschild; and the
Life Science Facility (LSF). J.C.M. is the recipient of a EMBO Long‐Term Fellowship
(ALTF number 710‐2016). This work was supported with MEYS CR, project no.CZ.02.1.01/0.0/0.0/16_019/0000738
to J.P., and by the Austrian Science Fund (FWF01_I1774S) to E.B.
article_number: e104238
article_processing_charge: Yes (via OA deal)
article_type: original
author:
- first_name: Juan C
full_name: Montesinos López, Juan C
id: 310A8E3E-F248-11E8-B48F-1D18A9856A87
last_name: Montesinos López
orcid: 0000-0001-9179-6099
- first_name: A
full_name: Abuzeineh, A
last_name: Abuzeineh
- first_name: Aglaja
full_name: Kopf, Aglaja
id: 31DAC7B6-F248-11E8-B48F-1D18A9856A87
last_name: Kopf
orcid: 0000-0002-2187-6656
- first_name: Alba
full_name: Juanes Garcia, Alba
id: 40F05888-F248-11E8-B48F-1D18A9856A87
last_name: Juanes Garcia
orcid: 0000-0002-1009-9652
- first_name: Krisztina
full_name: Ötvös, Krisztina
id: 29B901B0-F248-11E8-B48F-1D18A9856A87
last_name: Ötvös
orcid: 0000-0002-5503-4983
- first_name: J
full_name: Petrášek, J
last_name: Petrášek
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Montesinos López JC, Abuzeineh A, Kopf A, et al. Phytohormone cytokinin guides
microtubule dynamics during cell progression from proliferative to differentiated
stage. The Embo Journal. 2020;39(17). doi:10.15252/embj.2019104238
apa: Montesinos López, J. C., Abuzeineh, A., Kopf, A., Juanes Garcia, A., Ötvös,
K., Petrášek, J., … Benková, E. (2020). Phytohormone cytokinin guides microtubule
dynamics during cell progression from proliferative to differentiated stage. The
Embo Journal. Embo Press. https://doi.org/10.15252/embj.2019104238
chicago: Montesinos López, Juan C, A Abuzeineh, Aglaja Kopf, Alba Juanes Garcia,
Krisztina Ötvös, J Petrášek, Michael K Sixt, and Eva Benková. “Phytohormone Cytokinin
Guides Microtubule Dynamics during Cell Progression from Proliferative to Differentiated
Stage.” The Embo Journal. Embo Press, 2020. https://doi.org/10.15252/embj.2019104238.
ieee: J. C. Montesinos López et al., “Phytohormone cytokinin guides microtubule
dynamics during cell progression from proliferative to differentiated stage,”
The Embo Journal, vol. 39, no. 17. Embo Press, 2020.
ista: Montesinos López JC, Abuzeineh A, Kopf A, Juanes Garcia A, Ötvös K, Petrášek
J, Sixt MK, Benková E. 2020. Phytohormone cytokinin guides microtubule dynamics
during cell progression from proliferative to differentiated stage. The Embo Journal.
39(17), e104238.
mla: Montesinos López, Juan C., et al. “Phytohormone Cytokinin Guides Microtubule
Dynamics during Cell Progression from Proliferative to Differentiated Stage.”
The Embo Journal, vol. 39, no. 17, e104238, Embo Press, 2020, doi:10.15252/embj.2019104238.
short: J.C. Montesinos López, A. Abuzeineh, A. Kopf, A. Juanes Garcia, K. Ötvös,
J. Petrášek, M.K. Sixt, E. Benková, The Embo Journal 39 (2020).
corr_author: '1'
date_created: 2020-07-21T09:08:38Z
date_published: 2020-09-01T00:00:00Z
date_updated: 2025-04-15T06:37:27Z
day: '01'
ddc:
- '580'
department:
- _id: MiSi
- _id: EvBe
doi: 10.15252/embj.2019104238
external_id:
isi:
- '000548311800001'
pmid:
- '32667089'
file:
- access_level: open_access
checksum: 43d2b36598708e6ab05c69074e191d57
content_type: application/pdf
creator: dernst
date_created: 2020-12-02T09:13:23Z
date_updated: 2020-12-02T09:13:23Z
file_id: '8827'
file_name: 2020_EMBO_Montesinos.pdf
file_size: 3497156
relation: main_file
success: 1
file_date_updated: 2020-12-02T09:13:23Z
fulldoi: https://doi.org/10.15252/embj.2019104238
has_accepted_license: '1'
intvolume: ' 39'
isi: 1
issue: '17'
language:
- iso: eng
month: '09'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 253E54C8-B435-11E9-9278-68D0E5697425
grant_number: ALTF710-2016
name: Molecular mechanism of auxindriven formative divisions delineating lateral
root organogenesis in plants
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: The Embo Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Phytohormone cytokinin guides microtubule dynamics during cell progression
from proliferative to differentiated stage
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 39
year: '2020'
...
---
_id: '6980'
abstract:
- lang: eng
text: Tissue morphogenesis in multicellular organisms is brought about by spatiotemporal
coordination of mechanical and chemical signals. Extensive work on how mechanical
forces together with the well‐established morphogen signalling pathways can actively
shape living tissues has revealed evolutionary conserved mechanochemical features
of embryonic development. More recently, attention has been drawn to the description
of tissue material properties and how they can influence certain morphogenetic
processes. Interestingly, besides the role of tissue material properties in determining
how much tissues deform in response to force application, there is increasing
theoretical and experimental evidence, suggesting that tissue material properties
can abruptly and drastically change in development. These changes resemble phase
transitions, pointing at the intriguing possibility that important morphogenetic
processes in development, such as symmetry breaking and self‐organization, might
be mediated by tissue phase transitions. In this review, we summarize recent findings
on the regulation and role of tissue material properties in the context of the
developing embryo. We posit that abrupt changes of tissue rheological properties
may have important implications in maintaining the balance between robustness
and adaptability during embryonic development.
article_number: e102497
article_processing_charge: Yes (via OA deal)
article_type: review
author:
- first_name: Nicoletta
full_name: Petridou, Nicoletta
id: 2A003F6C-F248-11E8-B48F-1D18A9856A87
last_name: Petridou
orcid: 0000-0002-8451-1195
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: Petridou N, Heisenberg C-PJ. Tissue rheology in embryonic organization. The
EMBO Journal. 2019;38(20). doi:10.15252/embj.2019102497
apa: Petridou, N., & Heisenberg, C.-P. J. (2019). Tissue rheology in embryonic
organization. The EMBO Journal. Embo Press. https://doi.org/10.15252/embj.2019102497
chicago: Petridou, Nicoletta, and Carl-Philipp J Heisenberg. “Tissue Rheology in
Embryonic Organization.” The EMBO Journal. Embo Press, 2019. https://doi.org/10.15252/embj.2019102497.
ieee: N. Petridou and C.-P. J. Heisenberg, “Tissue rheology in embryonic organization,”
The EMBO Journal, vol. 38, no. 20. Embo Press, 2019.
ista: Petridou N, Heisenberg C-PJ. 2019. Tissue rheology in embryonic organization.
The EMBO Journal. 38(20), e102497.
mla: Petridou, Nicoletta, and Carl-Philipp J. Heisenberg. “Tissue Rheology in Embryonic
Organization.” The EMBO Journal, vol. 38, no. 20, e102497, Embo Press,
2019, doi:10.15252/embj.2019102497.
short: N. Petridou, C.-P.J. Heisenberg, The EMBO Journal 38 (2019).
corr_author: '1'
date_created: 2019-11-04T15:24:29Z
date_published: 2019-10-15T00:00:00Z
date_updated: 2025-05-14T11:21:32Z
day: '15'
ddc:
- '570'
department:
- _id: CaHe
doi: 10.15252/embj.2019102497
ec_funded: 1
external_id:
isi:
- '000485561900001'
pmid:
- '31512749'
file:
- access_level: open_access
checksum: 76f7f4e79ab6d850c30017a69726fd85
content_type: application/pdf
creator: dernst
date_created: 2019-11-04T15:30:08Z
date_updated: 2020-07-14T12:47:46Z
file_id: '6981'
file_name: 2019_Embo_Petridou.pdf
file_size: 847356
relation: main_file
file_date_updated: 2020-07-14T12:47:46Z
fulldoi: https://doi.org/10.15252/embj.2019102497
has_accepted_license: '1'
intvolume: ' 38'
isi: 1
issue: '20'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
- _id: 2693FD8C-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: V00736
name: Tissue material properties in embryonic development
publication: The EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Embo Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Tissue rheology in embryonic organization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 38
year: '2019'
...
---
_id: '145'
abstract:
- lang: eng
text: Aged proteins can become hazardous to cellular function, by accumulating molecular
damage. This implies that cells should preferentially rely on newly produced ones.
We tested this hypothesis in cultured hippocampal neurons, focusing on synaptic
transmission. We found that newly synthesized vesicle proteins were incorporated
in the actively recycling pool of vesicles responsible for all neurotransmitter
release during physiological activity. We observed this for the calcium sensor
Synaptotagmin 1, for the neurotransmitter transporter VGAT, and for the fusion
protein VAMP2 (Synaptobrevin 2). Metabolic labeling of proteins and visualization
by secondary ion mass spectrometry enabled us to query the entire protein makeup
of the actively recycling vesicles, which we found to be younger than that of
non-recycling vesicles. The young vesicle proteins remained in use for up to ~
24 h, during which they participated in recycling a few hundred times. They were
afterward reluctant to release and were degraded after an additional ~ 24–48 h.
We suggest that the recycling pool of synaptic vesicles relies on newly synthesized
proteins, while the inactive reserve pool contains older proteins.
acknowledgement: We thank Reinhard Jahn for providing a plasmid for YFP-SNAP25. We
thank Erwin Neher for help with the development of the mathematical model of the
synaptic vesicle life cycle. We thank Martin Meschkat, Andreas Höbartner, Annedore
Punge, and Peer Hoopmann for help with the experiments. We thank Burkhard Rammner
for providing the illustrations of synaptic vesicle and protein dynamics. We thank
Manuel Maidorn, Martin Helm, and Katharina N. Richter for critically reading the
manuscript. S.T. was supported by an Excellence Stipend of the Göttingen Graduate
School for Neurosciences, Biophysics, and Molecular Biosciences (GGNB). E.F.F. is
a recipient of long-term fellowships from the European Molecular Biology Organization
(ALTF_797-2012) and from the Human Frontier Science Program (HFSP_LT000830/2013).
The work was supported by grants to S.O.R. from the European Research Council (ERC-2013-CoG
NeuroMolAnatomy) and from the Deutsche Forschungsgemeinschaft (Cluster of Excellence
Nanoscale Microscopy and Molecular Physiology of the Brain, SFB1190/P09, SFB889/A05,
and SFB1286/A03, and DFG RI 1967 7/1). The nanoSIMS instrument was funded by the
German Federal Ministry of Education and Research (03F0626A).
article_number: e98044
article_processing_charge: No
article_type: original
author:
- first_name: Sven M
full_name: Truckenbrodt, Sven M
id: 45812BD4-F248-11E8-B48F-1D18A9856A87
last_name: Truckenbrodt
- first_name: Abhiyan
full_name: Viplav, Abhiyan
last_name: Viplav
- first_name: Sebsatian
full_name: Jähne, Sebsatian
last_name: Jähne
- first_name: Angela
full_name: Vogts, Angela
last_name: Vogts
- first_name: Annette
full_name: Denker, Annette
last_name: Denker
- first_name: Hanna
full_name: Wildhagen, Hanna
last_name: Wildhagen
- first_name: Eugenio
full_name: Fornasiero, Eugenio
last_name: Fornasiero
- first_name: Silvio
full_name: Rizzoli, Silvio
last_name: Rizzoli
citation:
ama: Truckenbrodt SM, Viplav A, Jähne S, et al. Newly produced synaptic vesicle
proteins are preferentially used in synaptic transmission. The EMBO Journal.
2018;37(15). doi:10.15252/embj.201798044
apa: Truckenbrodt, S. M., Viplav, A., Jähne, S., Vogts, A., Denker, A., Wildhagen,
H., … Rizzoli, S. (2018). Newly produced synaptic vesicle proteins are preferentially
used in synaptic transmission. The EMBO Journal. Wiley. https://doi.org/10.15252/embj.201798044
chicago: Truckenbrodt, Sven M, Abhiyan Viplav, Sebsatian Jähne, Angela Vogts, Annette
Denker, Hanna Wildhagen, Eugenio Fornasiero, and Silvio Rizzoli. “Newly Produced
Synaptic Vesicle Proteins Are Preferentially Used in Synaptic Transmission.” The
EMBO Journal. Wiley, 2018. https://doi.org/10.15252/embj.201798044.
ieee: S. M. Truckenbrodt et al., “Newly produced synaptic vesicle proteins
are preferentially used in synaptic transmission,” The EMBO Journal, vol.
37, no. 15. Wiley, 2018.
ista: Truckenbrodt SM, Viplav A, Jähne S, Vogts A, Denker A, Wildhagen H, Fornasiero
E, Rizzoli S. 2018. Newly produced synaptic vesicle proteins are preferentially
used in synaptic transmission. The EMBO Journal. 37(15), e98044.
mla: Truckenbrodt, Sven M., et al. “Newly Produced Synaptic Vesicle Proteins Are
Preferentially Used in Synaptic Transmission.” The EMBO Journal, vol. 37,
no. 15, e98044, Wiley, 2018, doi:10.15252/embj.201798044.
short: S.M. Truckenbrodt, A. Viplav, S. Jähne, A. Vogts, A. Denker, H. Wildhagen,
E. Fornasiero, S. Rizzoli, The EMBO Journal 37 (2018).
corr_author: '1'
date_created: 2018-12-11T11:44:52Z
date_published: 2018-08-01T00:00:00Z
date_updated: 2024-10-09T20:58:32Z
day: '01'
ddc:
- '570'
department:
- _id: JoDa
doi: 10.15252/embj.201798044
external_id:
isi:
- '000440416900005'
pmid:
- '29950309'
file:
- access_level: open_access
checksum: a540feb6c9af6aefc78de531461a8835
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T14:17:29Z
date_updated: 2020-07-14T12:44:56Z
file_id: '5710'
file_name: 2018_EMBO_Truckenbrodt.pdf
file_size: 2846470
relation: main_file
file_date_updated: 2020-07-14T12:44:56Z
fulldoi: https://doi.org/10.15252/embj.201798044
has_accepted_license: '1'
intvolume: ' 37'
isi: 1
issue: '15'
language:
- iso: eng
month: '08'
oa: 1
oa_version: Published Version
pmid: 1
publication: The EMBO Journal
publication_identifier:
issn:
- 0261-4189
publication_status: published
publisher: Wiley
publist_id: '7778'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Newly produced synaptic vesicle proteins are preferentially used in synaptic
transmission
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 37
year: '2018'
...
---
_id: '2867'
abstract:
- lang: eng
text: The plant hormone auxin elicits many specific context-dependent developmental
responses. Auxin promotes degradation of Aux/IAA proteins that prevent transcription
factors of the auxin response factor (ARF) family from regulating auxin-responsive
target genes. Aux/IAAs and ARFs are represented by large gene families in Arabidopsis.
Here we show that stabilization of BDL/IAA12 or its sister protein IAA13 prevents
MP/ARF5-dependent embryonic root formation whereas stabilized SHY2/IAA3 interferes
with seedling growth. Although both bdl and shy2-2 proteins inhibited MP/ARF5-dependent
reporter gene activation, shy2-2 was much less efficient than bdl to interfere
with embryonic root initiation when expressed from the BDL promoter. Similarly,
MP was much more efficient than ARF16 in this process. When expressed from the
SHY2 promoter, both shy2-2 and bdl inhibited cell elongation and auxin-induced
gene expression in the seedling hypocotyl. By contrast, gravitropism and auxin-induced
gene expression in the root, which were promoted by functionally redundant NPH4/ARF7
and ARF19 proteins, were inhibited by shy2-2, but not by bdl protein. Our results
suggest that auxin signals are converted into specific responses by matching pairs
of coexpressed ARF and Aux/IAA proteins.
article_processing_charge: No
article_type: original
author:
- first_name: Dolf
full_name: Weijers, Dolf
last_name: Weijers
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
- first_name: Katja
full_name: Jäger, Katja
last_name: Jäger
- first_name: Alexandra
full_name: Schlereth, Alexandra
last_name: Schlereth
- first_name: Thorsten
full_name: Hamann, Thorsten
last_name: Hamann
- first_name: Marika
full_name: Kientz, Marika
last_name: Kientz
- first_name: Jill
full_name: Wilmoth, Jill
last_name: Wilmoth
- first_name: Jason
full_name: Reed, Jason
last_name: Reed
- first_name: Gerd
full_name: Jürgens, Gerd
last_name: Jürgens
citation:
ama: Weijers D, Benková E, Jäger K, et al. Developmental specificity of auxin response
by pairs of ARF and Aux/IAA transcriptional regulators. EMBO Journal. 2005;24(10):1874-1885.
doi:10.1038/sj.emboj.7600659
apa: Weijers, D., Benková, E., Jäger, K., Schlereth, A., Hamann, T., Kientz, M.,
… Jürgens, G. (2005). Developmental specificity of auxin response by pairs of
ARF and Aux/IAA transcriptional regulators. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.1038/sj.emboj.7600659
chicago: Weijers, Dolf, Eva Benková, Katja Jäger, Alexandra Schlereth, Thorsten
Hamann, Marika Kientz, Jill Wilmoth, Jason Reed, and Gerd Jürgens. “Developmental
Specificity of Auxin Response by Pairs of ARF and Aux/IAA Transcriptional Regulators.”
EMBO Journal. Wiley-Blackwell, 2005. https://doi.org/10.1038/sj.emboj.7600659.
ieee: D. Weijers et al., “Developmental specificity of auxin response by
pairs of ARF and Aux/IAA transcriptional regulators,” EMBO Journal, vol.
24, no. 10. Wiley-Blackwell, pp. 1874–1885, 2005.
ista: Weijers D, Benková E, Jäger K, Schlereth A, Hamann T, Kientz M, Wilmoth J,
Reed J, Jürgens G. 2005. Developmental specificity of auxin response by pairs
of ARF and Aux/IAA transcriptional regulators. EMBO Journal. 24(10), 1874–1885.
mla: Weijers, Dolf, et al. “Developmental Specificity of Auxin Response by Pairs
of ARF and Aux/IAA Transcriptional Regulators.” EMBO Journal, vol. 24,
no. 10, Wiley-Blackwell, 2005, pp. 1874–85, doi:10.1038/sj.emboj.7600659.
short: D. Weijers, E. Benková, K. Jäger, A. Schlereth, T. Hamann, M. Kientz, J.
Wilmoth, J. Reed, G. Jürgens, EMBO Journal 24 (2005) 1874–1885.
das_tickbox: '1'
date_created: 2018-12-11T12:00:01Z
date_published: 2005-05-18T00:00:00Z
date_updated: 2026-07-15T13:21:20Z
day: '18'
doi: 10.1038/sj.emboj.7600659
extern: '1'
external_id:
pmid:
- '15889151'
fulldoi: https://doi.org/10.1038/sj.emboj.7600659
intvolume: ' 24'
issue: '10'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1142592/
month: '05'
oa: 1
oa_version: None
page: 1874 - 1885
pmid: 1
publication: EMBO Journal
publication_identifier:
eissn:
- 1460-2075
issn:
- 0261-4189
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3918'
status: public
title: Developmental specificity of auxin response by pairs of ARF and Aux/IAA transcriptional
regulators
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 24
year: '2005'
...
---
_id: '2981'
abstract:
- lang: eng
text: Plants contain a novel unique subfamily of Rho GTPases, vital components of
cellular signalling networks. Here we report a general role for some members of
this family in polarized plant growth processes. We show that Arabidopsis AtRop4
and AtRop6 encode functional GTPases with similar intrinsic GTP hydrolysis rates.
We localized AtRop proteins in root meristem cells to the cross-wall and cell
plate membranes. Polar localization of AtRops in trichoblasts specifies the growth
sites for emerging root hairs. These sites were visible before budding and elongation
of the Arabidopsis root hair when AtRops accumulated at their tips. Expression
of constitutively active AtRop4 and AtRop6 mutant proteins in root hairs of transgenic
Arabidopsis plants abolished polarized growth and delocalized the tip-focused
Ca2+ gradient. Polar localization of AtRops was inhibited by brefeldin A, but
not by other drugs such as latrunculin B, cytochalasin D or caffeine. Our results
demonstrate a general function of AtRop GTPases in tip growth and in polar diffuse
growth.
acknowledgement: We thank Drs Frantisek Baluška, Matthias Godde, Peter Huijser, Lars
Vahlkamp and Dieter Volkmann for help, criticism and constructive reading of the
manuscript. We are grateful to Dr N.-H.Chua for providing us with pTA7002. The work
was funded by the DFG, the European Communities Biotechnology Programme (Bio4-CT98
0239) and the INCO Copernicus Programme (IC15-CT96-0920). C.S.V.R. is the recipient
of an Alexander von Humboldt fellowship and J.F. of a DAAD fellowship.
article_processing_charge: No
article_type: original
author:
- first_name: Arthur
full_name: Molendijk, Arthur
last_name: Molendijk
- first_name: Friedrich
full_name: Bischoff, Friedrich
last_name: Bischoff
- first_name: Chadalavada
full_name: Rajendrakumar, Chadalavada
last_name: Rajendrakumar
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Markus
full_name: Braun, Markus
last_name: Braun
- first_name: Simon
full_name: Gilroy, Simon
last_name: Gilroy
- first_name: Klaus
full_name: Palme, Klaus
last_name: Palme
citation:
ama: Molendijk A, Bischoff F, Rajendrakumar C, et al. Arabidopsis thaliana Rop GTPases
are localized to tips of root hairs and control polar growth. EMBO Journal.
2001;20(11):2779-2788. doi:10.1093/emboj/20.11.2779
apa: Molendijk, A., Bischoff, F., Rajendrakumar, C., Friml, J., Braun, M., Gilroy,
S., & Palme, K. (2001). Arabidopsis thaliana Rop GTPases are localized to
tips of root hairs and control polar growth. EMBO Journal. Wiley-Blackwell.
https://doi.org/10.1093/emboj/20.11.2779
chicago: Molendijk, Arthur, Friedrich Bischoff, Chadalavada Rajendrakumar, Jiří
Friml, Markus Braun, Simon Gilroy, and Klaus Palme. “Arabidopsis Thaliana Rop
GTPases Are Localized to Tips of Root Hairs and Control Polar Growth.” EMBO
Journal. Wiley-Blackwell, 2001. https://doi.org/10.1093/emboj/20.11.2779.
ieee: A. Molendijk et al., “Arabidopsis thaliana Rop GTPases are localized
to tips of root hairs and control polar growth,” EMBO Journal, vol. 20,
no. 11. Wiley-Blackwell, pp. 2779–2788, 2001.
ista: Molendijk A, Bischoff F, Rajendrakumar C, Friml J, Braun M, Gilroy S, Palme
K. 2001. Arabidopsis thaliana Rop GTPases are localized to tips of root hairs
and control polar growth. EMBO Journal. 20(11), 2779–2788.
mla: Molendijk, Arthur, et al. “Arabidopsis Thaliana Rop GTPases Are Localized to
Tips of Root Hairs and Control Polar Growth.” EMBO Journal, vol. 20, no.
11, Wiley-Blackwell, 2001, pp. 2779–88, doi:10.1093/emboj/20.11.2779.
short: A. Molendijk, F. Bischoff, C. Rajendrakumar, J. Friml, M. Braun, S. Gilroy,
K. Palme, EMBO Journal 20 (2001) 2779–2788.
date_created: 2018-12-11T12:00:40Z
date_published: 2001-06-01T00:00:00Z
date_updated: 2023-05-16T12:07:45Z
day: '01'
doi: 10.1093/emboj/20.11.2779
extern: '1'
external_id:
pmid:
- '11387211'
fulldoi: https://doi.org/10.1093/emboj/20.11.2779
intvolume: ' 20'
issue: '11'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125484/
month: '06'
oa: 1
oa_version: Published Version
page: 2779 - 2788
pmid: 1
publication: EMBO Journal
publication_identifier:
issn:
- 0261-4189
publication_status: published
publisher: Wiley-Blackwell
publist_id: '3721'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Arabidopsis thaliana Rop GTPases are localized to tips of root hairs and control
polar growth
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 20
year: '2001'
...
---
_id: '1955'
abstract:
- lang: eng
text: 'The plastid genomes of several plants contain homologues, termed ndh genes,
of genes encoding subunits of the NADH:ubiquinone oxidoreductase or complex I
of mitochondria and eubacteria. The functional significance of the Ndh proteins
in higher plants is uncertain. We show here that tobacco chloroplasts contain
a protein complex of 550 kDa consisting of at least three of the ndh gene products:
NdhI, NdhJ and NdhK. We have constructed mutant tobacco plants with disrupted
ndhC, ndhK and ndhJ plastid genes, indicating that the Ndh complex is dispensible
for plant growth under optimal growth conditions. Chlorophyll fluorescence analysis
shows that in vivo the Ndh complex catalyses the post-illumination reduction of
the plastoquinone pool and in the light optimizes the induction of photosynthesis
under conditions of water stress. We conclude that the Ndh complex catalyses the
reduction of the plastoquinone pool using stromal reductant and so acts as a respiratory
complex. Overall, our data are compatible with the participation of the Ndh complex
in cyclic electron flow around the photosystem I complex in the light and possibly
in a chloroplast respiratory chain in the dark.'
acknowledgement: We thank Professor Süss (Institute of Plant Genetics and Crop Plant
Research, Gatersleben, Germany) for the gift of the anti-FNR antiserum, Professor
Masahiro Sugiura (Nagoya University, Japan) for the gift of plasmid pTB19 and Professor
Peter Horton (University of Sheffield) for the loan of his ED-800T unit. P.B. is
a recipient of a BBSRC studentship and the work was supported by grants from the
BBSRC, The Royal Society (to P.J.N.) and The National Science Foundation (to P.M.).
article_processing_charge: No
article_type: original
author:
- first_name: Paul
full_name: Burrows, Paul
last_name: Burrows
- first_name: Leonid A
full_name: Sazanov, Leonid A
id: 338D39FE-F248-11E8-B48F-1D18A9856A87
last_name: Sazanov
orcid: 0000-0002-0977-7989
- first_name: Zóra
full_name: Sváb, Zóra
last_name: Sváb
- first_name: Pàl
full_name: Maliga, Pàl
last_name: Maliga
- first_name: Peter
full_name: Nixon, Peter
last_name: Nixon
citation:
ama: Burrows P, Sazanov LA, Sváb Z, Maliga P, Nixon P. Identification of a functional
respiratory complex in chloroplasts through analysis of tobacco mutants containing
disrupted plastid ndh genes. EMBO Journal. 1998;17(4):868-876. doi:10.1093/emboj/17.4.868
apa: Burrows, P., Sazanov, L. A., Sváb, Z., Maliga, P., & Nixon, P. (1998).
Identification of a functional respiratory complex in chloroplasts through analysis
of tobacco mutants containing disrupted plastid ndh genes. EMBO Journal.
Wiley-Blackwell. https://doi.org/10.1093/emboj/17.4.868
chicago: Burrows, Paul, Leonid A Sazanov, Zóra Sváb, Pàl Maliga, and Peter Nixon.
“Identification of a Functional Respiratory Complex in Chloroplasts through Analysis
of Tobacco Mutants Containing Disrupted Plastid Ndh Genes.” EMBO Journal.
Wiley-Blackwell, 1998. https://doi.org/10.1093/emboj/17.4.868.
ieee: P. Burrows, L. A. Sazanov, Z. Sváb, P. Maliga, and P. Nixon, “Identification
of a functional respiratory complex in chloroplasts through analysis of tobacco
mutants containing disrupted plastid ndh genes,” EMBO Journal, vol. 17,
no. 4. Wiley-Blackwell, pp. 868–876, 1998.
ista: Burrows P, Sazanov LA, Sváb Z, Maliga P, Nixon P. 1998. Identification of
a functional respiratory complex in chloroplasts through analysis of tobacco mutants
containing disrupted plastid ndh genes. EMBO Journal. 17(4), 868–876.
mla: Burrows, Paul, et al. “Identification of a Functional Respiratory Complex in
Chloroplasts through Analysis of Tobacco Mutants Containing Disrupted Plastid
Ndh Genes.” EMBO Journal, vol. 17, no. 4, Wiley-Blackwell, 1998, pp. 868–76,
doi:10.1093/emboj/17.4.868.
short: P. Burrows, L.A. Sazanov, Z. Sváb, P. Maliga, P. Nixon, EMBO Journal 17 (1998)
868–876.
date_created: 2018-12-11T11:54:54Z
date_published: 1998-02-04T00:00:00Z
date_updated: 2022-09-01T13:17:49Z
day: '04'
doi: 10.1093/emboj/17.4.868
extern: '1'
external_id:
pmid:
- '9463365'
fulldoi: https://doi.org/10.1093/emboj/17.4.868
intvolume: ' 17'
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1170436/
month: '02'
oa: 1
oa_version: None
page: 868 - 876
pmid: 1
publication: EMBO Journal
publication_identifier:
issn:
- 0261-4189
publication_status: published
publisher: Wiley-Blackwell
publist_id: '5129'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Identification of a functional respiratory complex in chloroplasts through
analysis of tobacco mutants containing disrupted plastid ndh genes
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 17
year: '1998'
...