[{"fulldoi":"https://doi.org/10.2144/000112296","author":[{"first_name":"Davide","last_name":"Tonelli","full_name":"Tonelli, Davide"},{"last_name":"Calegari","full_name":"Calegari, Frederico","first_name":"Frederico"},{"first_name":"Ji","last_name":"Fei","full_name":"Fei, Ji"},{"last_name":"Nomura","full_name":"Nomura, Tadashi","first_name":"Tadashi"},{"last_name":"Osumi","full_name":"Osumi, Noriko","first_name":"Noriko"},{"last_name":"Heisenberg","full_name":"Heisenberg, Carl-Philipp J","id":"39427864-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-0912-4566","first_name":"Carl-Philipp J"},{"full_name":"Huttner, Wieland","last_name":"Huttner","first_name":"Wieland"}],"article_type":"original","oa_version":"None","publication_identifier":{"issn":["0736-6205"],"eissn":["1940-9818"]},"publist_id":"1974","extern":"1","day":"01","type":"journal_article","date_published":"2006-12-01T00:00:00Z","status":"public","publication_status":"published","article_processing_charge":"No","year":"2006","publisher":"Informa Healthcare","abstract":[{"text":"The detection of microRNAs (miRNAs) at single-cell resolution is important for studying the role of these posttranscriptional regulators. Here, we use a dual-fluorescent green fluorescent protein (GFP)-reporter/monomeric red fluorescent protein (mRFP)-sensor (DFRS) plasmid, injected into zebrafish blastomeres or electroporated into defined tissues of mouse embryos in utero or ex utero, to monitor the dynamics of specific miRNAs in individual live cells. This approach reveals, for example, that in the developing mouse central nervous system,, miR-124a is expressed not only in postmitotic neurons but also in neuronal progenitor cells. Collectively, our results demonstrate that acute administration of DFRS plasmids.offers an alternative to previous in situ hybridization and transgenic approaches and allows the monitoring of miRNA appearance and disappearance in defined cell lineages during vertebrate development.","lang":"eng"}],"month":"12","page":"727 - 732","_id":"4145","volume":41,"citation":{"ama":"Tonelli D, Calegari F, Fei J, et al. Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. <i>Biotechniques</i>. 2006;41(6):727-732. doi:<a href=\"https://doi.org/10.2144/000112296\">10.2144/000112296</a>","chicago":"Tonelli, Davide, Frederico Calegari, Ji Fei, Tadashi Nomura, Noriko Osumi, Carl-Philipp J Heisenberg, and Wieland Huttner. “Single-Cell Detection of MicroRNAs in Developing Vertebrate Embryos after Acute Administration of a Dual-Fluorescence Reporter/Sensor Plasmid.” <i>Biotechniques</i>. Informa Healthcare, 2006. <a href=\"https://doi.org/10.2144/000112296\">https://doi.org/10.2144/000112296</a>.","apa":"Tonelli, D., Calegari, F., Fei, J., Nomura, T., Osumi, N., Heisenberg, C.-P. J., &#38; Huttner, W. (2006). Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. <i>Biotechniques</i>. Informa Healthcare. <a href=\"https://doi.org/10.2144/000112296\">https://doi.org/10.2144/000112296</a>","short":"D. Tonelli, F. Calegari, J. Fei, T. Nomura, N. Osumi, C.-P.J. Heisenberg, W. Huttner, Biotechniques 41 (2006) 727–732.","ista":"Tonelli D, Calegari F, Fei J, Nomura T, Osumi N, Heisenberg C-PJ, Huttner W. 2006. Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid. Biotechniques. 41(6), 727–732.","mla":"Tonelli, Davide, et al. “Single-Cell Detection of MicroRNAs in Developing Vertebrate Embryos after Acute Administration of a Dual-Fluorescence Reporter/Sensor Plasmid.” <i>Biotechniques</i>, vol. 41, no. 6, Informa Healthcare, 2006, pp. 727–32, doi:<a href=\"https://doi.org/10.2144/000112296\">10.2144/000112296</a>.","ieee":"D. Tonelli <i>et al.</i>, “Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid,” <i>Biotechniques</i>, vol. 41, no. 6. Informa Healthcare, pp. 727–732, 2006."},"publication":"Biotechniques","date_updated":"2026-08-26T09:09:45Z","OA_type":"closed access","title":"Single-cell detection of microRNAs in developing vertebrate embryos after acute administration of a dual-fluorescence reporter/sensor plasmid","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","doi":"10.2144/000112296","language":[{"iso":"eng"}],"intvolume":"        41","issue":"6","pmid":1,"external_id":{"pmid":["17191618 "]},"date_created":"2018-12-11T12:07:12Z"},{"year":"2002","article_processing_charge":"No","publisher":"Informa Healthcare","abstract":[{"text":"Quantitative real-time PCR represents a highly sensitive and powerful technique for the quantitation of nucleic acids. It has a tremendous potential for the high-throughput analysis of gene expression in research and routine diagnostics. However, the major hurdle is not the practical performance of the experiments themselves but rather the efficient evaluation and the mathematical and statistical analysis of the enormous amount of data gained by this technology, as these functions are not included in the software provided by the manufacturers of the detection systems. In this work, we focus on the mathematical evaluation and analysis of the data generated by quantitative real-time PCR, the calculation of the final results, the propagation of experimental variation of the measured values to the final results, and the statistical analysis. We developed a Microsoft Excel-based software application coded in Visual Basic for Applications, called Q-Gene, which addresses these points. Q-Gene manages and expedites the planning, performance, and evaluation of quantitative real-time PCR experiments, as well as the mathematical and statistical analysis, storage, and graphical presentation of the data. The Q-Gene software application is a tool to cope with complex quantitative real-time PCR experiments at a high-throughput scale and considerably expedites and rationalizes the experimental setup, data analysis, and data management while ensuring highest reproducibility.","lang":"eng"}],"month":"06","type":"journal_article","date_published":"2002-06-01T00:00:00Z","status":"public","publication_status":"published","oa_version":"None","publication_identifier":{"issn":["0736-6205"]},"publist_id":"2979","extern":"1","day":"01","author":[{"last_name":"Müller","full_name":"Müller, Patrick","first_name":"Patrick"},{"last_name":"Janovjak","full_name":"Janovjak, Harald L","id":"33BA6C30-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8023-9315","first_name":"Harald L"},{"first_name":"Andre","full_name":"Miserez, Andre","last_name":"Miserez"},{"full_name":"Dobbie, Zuzana","last_name":"Dobbie","first_name":"Zuzana"}],"article_type":"original","issue":"6","pmid":1,"external_id":{"pmid":["12074169"]},"quality_controlled":"1","date_created":"2018-12-11T12:03:15Z","language":[{"iso":"eng"}],"intvolume":"        32","scopus_import":"1","date_updated":"2023-07-17T11:29:06Z","title":"Processing of gene expression data generated by quantitative real-time RT-PCR","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","page":"1372 - 1379","_id":"3422","volume":32,"citation":{"ista":"Müller P, Janovjak HL, Miserez A, Dobbie Z. 2002. Processing of gene expression data generated by quantitative real-time RT-PCR. Biotechniques. 32(6), 1372–1379.","short":"P. Müller, H.L. Janovjak, A. Miserez, Z. Dobbie, Biotechniques 32 (2002) 1372–1379.","mla":"Müller, Patrick, et al. “Processing of Gene Expression Data Generated by Quantitative Real-Time RT-PCR.” <i>Biotechniques</i>, vol. 32, no. 6, Informa Healthcare, 2002, pp. 1372–79.","ieee":"P. Müller, H. L. Janovjak, A. Miserez, and Z. Dobbie, “Processing of gene expression data generated by quantitative real-time RT-PCR,” <i>Biotechniques</i>, vol. 32, no. 6. Informa Healthcare, pp. 1372–1379, 2002.","chicago":"Müller, Patrick, Harald L Janovjak, Andre Miserez, and Zuzana Dobbie. “Processing of Gene Expression Data Generated by Quantitative Real-Time RT-PCR.” <i>Biotechniques</i>. Informa Healthcare, 2002.","ama":"Müller P, Janovjak HL, Miserez A, Dobbie Z. Processing of gene expression data generated by quantitative real-time RT-PCR. <i>Biotechniques</i>. 2002;32(6):1372-1379.","apa":"Müller, P., Janovjak, H. L., Miserez, A., &#38; Dobbie, Z. (2002). Processing of gene expression data generated by quantitative real-time RT-PCR. <i>Biotechniques</i>. Informa Healthcare."},"publication":"Biotechniques"}]