---
OA_place: publisher
OA_type: hybrid
_id: '18170'
abstract:
- lang: eng
text: 'This study presents a graphene field-effect transistor (gFET) biosensor with
dual detection capabilities for SARS-CoV-2: one RNA detection assay to confirm
viral positivity and the other for nucleocapsid (N-)protein detection as a proxy
for infectiousness of the patient. This technology can be rapidly adapted to emerging
infectious diseases, making an essential tool to contain future pandemics. To
detect viral RNA, the highly conserved E-gene of the virus was targeted, allowing
for the determination of SARS-CoV-2 presence or absence using nasopharyngeal swab
samples. For N-protein detection, specific antibodies were used. Tested on 213
clinical nasopharyngeal samples, the gFET biosensor showed good correlation with
RT-PCR cycle threshold values, proving its high sensitivity in detecting SARS-CoV-2
RNA. Specificity was confirmed using 21 pre-pandemic samples positive for other
respiratory viruses. The gFET biosensor had a limit of detection (LOD) for N-protein
of 0.9 pM, establishing a foundation for the development of a sensitive tool for
monitoring active viral infection. Results of gFET based N-protein detection corresponded
to the results of virus culture in all 16 available clinical samples and thus
it also proved its capability to serve as a proxy for infectivity. Overall, these
findings support the potential of the gFET biosensor as a point-of-care device
for rapid diagnosis of SARS-CoV-2 infection and indirect assessment of infectiousness
in patients, providing additional information for clinical and public health decision-making.'
acknowledgement: 'This research was funded in whole by the Austrian Science Fund (FWF)
[P 35103-B, Grant-DOI: 10.55776/P35103]. For open access purposes, the author has
applied a CC BY public copyright license to any author-accepted manuscript version
arising from this submission. We would like to thank Olfert Landt for advice on
ssDNA probe design; Rui Qiang Chen, Jennifer Stock, and Christine Wukotitsch for
their excellent support with ONT sequencing; Christoph Köppl and Andreas Fischer
for excellent support in recombinant N protein expression and purification; and
the whole team at the division of clinical virology for their support with standard
diagnostics.'
article_number: '116807'
article_processing_charge: Yes (in subscription journal)
article_type: original
author:
- first_name: Anna Nele
full_name: Herdina, Anna Nele
last_name: Herdina
- first_name: Anil
full_name: Bozdogan, Anil
last_name: Bozdogan
- first_name: Patrik
full_name: Aspermair, Patrik
last_name: Aspermair
- first_name: Jakub
full_name: Dostalek, Jakub
last_name: Dostalek
- first_name: Miriam
full_name: Klausberger, Miriam
last_name: Klausberger
- first_name: Nico
full_name: Lingg, Nico
last_name: Lingg
- first_name: Monika
full_name: Cserjan-Puschmann, Monika
last_name: Cserjan-Puschmann
- first_name: Patricia Pereira
full_name: Aguilar, Patricia Pereira
last_name: Aguilar
- first_name: Simone
full_name: Auer, Simone
last_name: Auer
- first_name: Halil
full_name: Demirtas, Halil
last_name: Demirtas
- first_name: Jakob
full_name: Andersson, Jakob
id: 3a5f4167-9bd9-11ed-bd12-a1446d38776f
last_name: Andersson
- first_name: Felix
full_name: Lötsch, Felix
last_name: Lötsch
- first_name: Barbara
full_name: Holzer, Barbara
last_name: Holzer
- first_name: Adi
full_name: Steinrigl, Adi
last_name: Steinrigl
- first_name: Florian
full_name: Thalhammer, Florian
last_name: Thalhammer
- first_name: Julia
full_name: Schellnegger, Julia
last_name: Schellnegger
- first_name: Monika
full_name: Breuer, Monika
last_name: Breuer
- first_name: Wolfgang
full_name: Knoll, Wolfgang
last_name: Knoll
- first_name: Robert
full_name: Strassl, Robert
last_name: Strassl
citation:
ama: 'Herdina AN, Bozdogan A, Aspermair P, et al. Bridging basic science and applied
diagnostics: Comprehensive viral diagnostics enabled by graphene-based electronic
biosensor technology advancements. Biosensors and Bioelectronics. 2025;267.
doi:10.1016/j.bios.2024.116807'
apa: 'Herdina, A. N., Bozdogan, A., Aspermair, P., Dostalek, J., Klausberger, M.,
Lingg, N., … Strassl, R. (2025). Bridging basic science and applied diagnostics:
Comprehensive viral diagnostics enabled by graphene-based electronic biosensor
technology advancements. Biosensors and Bioelectronics. Elsevier. https://doi.org/10.1016/j.bios.2024.116807'
chicago: 'Herdina, Anna Nele, Anil Bozdogan, Patrik Aspermair, Jakub Dostalek, Miriam
Klausberger, Nico Lingg, Monika Cserjan-Puschmann, et al. “Bridging Basic Science
and Applied Diagnostics: Comprehensive Viral Diagnostics Enabled by Graphene-Based
Electronic Biosensor Technology Advancements.” Biosensors and Bioelectronics.
Elsevier, 2025. https://doi.org/10.1016/j.bios.2024.116807.'
ieee: 'A. N. Herdina et al., “Bridging basic science and applied diagnostics:
Comprehensive viral diagnostics enabled by graphene-based electronic biosensor
technology advancements,” Biosensors and Bioelectronics, vol. 267. Elsevier,
2025.'
ista: 'Herdina AN, Bozdogan A, Aspermair P, Dostalek J, Klausberger M, Lingg N,
Cserjan-Puschmann M, Aguilar PP, Auer S, Demirtas H, Andersson J, Lötsch F, Holzer
B, Steinrigl A, Thalhammer F, Schellnegger J, Breuer M, Knoll W, Strassl R. 2025.
Bridging basic science and applied diagnostics: Comprehensive viral diagnostics
enabled by graphene-based electronic biosensor technology advancements. Biosensors
and Bioelectronics. 267, 116807.'
mla: 'Herdina, Anna Nele, et al. “Bridging Basic Science and Applied Diagnostics:
Comprehensive Viral Diagnostics Enabled by Graphene-Based Electronic Biosensor
Technology Advancements.” Biosensors and Bioelectronics, vol. 267, 116807,
Elsevier, 2025, doi:10.1016/j.bios.2024.116807.'
short: A.N. Herdina, A. Bozdogan, P. Aspermair, J. Dostalek, M. Klausberger, N.
Lingg, M. Cserjan-Puschmann, P.P. Aguilar, S. Auer, H. Demirtas, J. Andersson,
F. Lötsch, B. Holzer, A. Steinrigl, F. Thalhammer, J. Schellnegger, M. Breuer,
W. Knoll, R. Strassl, Biosensors and Bioelectronics 267 (2025).
date_created: 2024-10-06T22:01:11Z
date_published: 2025-01-01T00:00:00Z
date_updated: 2025-02-27T12:34:07Z
day: '01'
ddc:
- '570'
department:
- _id: LeSa
doi: 10.1016/j.bios.2024.116807
external_id:
isi:
- '001328413700001'
pmid:
- '39341071'
file:
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checksum: 208ac27dab27af792d198fcb74af8756
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creator: dernst
date_created: 2025-01-13T11:14:32Z
date_updated: 2025-01-13T11:14:32Z
file_id: '18843'
file_name: 2025_BiosensorsBioelectronics_Herdina.pdf
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relation: main_file
success: 1
file_date_updated: 2025-01-13T11:14:32Z
has_accepted_license: '1'
intvolume: ' 267'
isi: 1
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '01'
oa: 1
oa_version: Published Version
pmid: 1
publication: Biosensors and Bioelectronics
publication_identifier:
eissn:
- 1873-4235
issn:
- 0956-5663
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Bridging basic science and applied diagnostics: Comprehensive viral diagnostics
enabled by graphene-based electronic biosensor technology advancements'
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 267
year: '2025'
...