[{"external_id":{"isi":["001547884300001"],"pmid":["40782342"]},"article_processing_charge":"Yes (via OA deal)","project":[{"_id":"bd76d395-d553-11ed-ba76-f678c14f9033","grant_number":"I06123","name":"Peptide receptors for auxin canalization in Arabidopsis"}],"acknowledged_ssus":[{"_id":"Bio"},{"_id":"EM-Fac"},{"_id":"LifeSc"}],"article_number":"e70396","title":"Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development","volume":123,"abstract":[{"text":"Very long-chain fatty acids (VLCFAs), being constituents of different types of lipids, are critical factors in plant development, presumably due to their impact on the endomembrane system. The VLCFAs are synthesized in the endoplasmic reticulum by a heterotetrameric enzymatic complex including β-ketoacyl CoA reductase 1 (KCR1), whose mutant is lethal. Here, we describe the ectopic shoot meristems (esm) mutant, a viable kcr1 allele presumably affecting surface properties of the KCR1 protein. This kcr1-2 mutant shows reduced fatty acyl elongation that impacts VLCFAs. The kcr1-2 plants show severe defects during different stages of development, which all correlate with defects in polar localization and subcellular trafficking of PIN auxin transporters and resulting asymmetric auxin distribution. Detailed analysis of KCR1 expression and patterning defects in kcr1-2 suggests that KCR1 plays a role in delineating boundaries around meristematic and specialized differentiating tissues, including root and shoot meristems, initiating lateral roots, lateral root primordia, and trichomes. In these contexts, KCR1-produced VLCFAs may act in a non-cell-autonomous manner. Viable kcr1-2 represents a useful tool to study VLCFA roles in plant development and highlights VLCFAs as critical developmental factors at the interface of cell polarity and tissue development.","lang":"eng"}],"publication":"Plant Journal","file_date_updated":"2025-09-01T14:09:31Z","oa_version":"Published Version","oa":1,"department":[{"_id":"EvBe"},{"_id":"JiFr"},{"_id":"GradSch"}],"OA_place":"publisher","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"3","type":"journal_article","user_id":"317138e5-6ab7-11ef-aa6d-ffef3953e345","month":"08","citation":{"ista":"Babic D, Abualia R, Fiedler L, Qi L, Tellier F, Smoljan A, Rakusova H, Valošek P, Han H, Benková E, Faure JD, Friml J. 2025. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. Plant Journal. 123(3), e70396.","apa":"Babic, D., Abualia, R., Fiedler, L., Qi, L., Tellier, F., Smoljan, A., … Friml, J. (2025). Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>","short":"D. Babic, R. Abualia, L. Fiedler, L. Qi, F. Tellier, A. Smoljan, H. Rakusova, P. Valošek, H. Han, E. Benková, J.D. Faure, J. Friml, Plant Journal 123 (2025).","mla":"Babic, David, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>, vol. 123, no. 3, e70396, Wiley, 2025, doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>.","ieee":"D. Babic <i>et al.</i>, “Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development,” <i>Plant Journal</i>, vol. 123, no. 3. Wiley, 2025.","ama":"Babic D, Abualia R, Fiedler L, et al. Biosynthesis of very long-chain fatty acids is required for Arabidopsis auxin-mediated embryonic and post-embryonic development. <i>Plant Journal</i>. 2025;123(3). doi:<a href=\"https://doi.org/10.1111/tpj.70396\">10.1111/tpj.70396</a>","chicago":"Babic, David, Rashed Abualia, Lukas Fiedler, Linlin Qi, Frédérique Tellier, Adrijana Smoljan, Hana Rakusova, et al. “Biosynthesis of Very Long-Chain Fatty Acids Is Required for Arabidopsis Auxin-Mediated Embryonic and Post-Embryonic Development.” <i>Plant Journal</i>. Wiley, 2025. <a href=\"https://doi.org/10.1111/tpj.70396\">https://doi.org/10.1111/tpj.70396</a>."},"scopus_import":"1","acknowledgement":"We gratefully acknowledge the Imaging and Optics, Electron Microscopy (especially Vanessa Zheden for technical assistance) and Life Science (in particular Dorota Jaworska) facilities at ISTA for their continuous support. Authors would like to thank Michelle Gallei for advice during the generation of the transgenic lines; Zuzana Gelová for advice with DR5rev::GFP analyses; Ivan Kulich for help and advice on trichome imaging; Aline Monzer for generous help with hypocotyl and root analyses; Shutang Tan for help with the NGS data analysis; and Milan Župunski for advice on abiotic stress experiments. We would like to thank Dolf Weijers for the SOSEKI (SOK) marker line seeds. This work has benefited from the support of IJPB's Plant Observatory platforms P0-Chem.\r\n\r\nThis work was supported by Austrian Science Fund (FWF) (I 6123-B) and Science and Technology Department of Jiangxi Province (20223BCJ25037) to Huibin Han. The IJPB benefits from the support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).","_id":"20187","publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"related_material":{"record":[{"relation":"dissertation_contains","status":"public","id":"20362"}]},"date_created":"2025-08-17T22:01:36Z","doi":"10.1111/tpj.70396","has_accepted_license":"1","intvolume":"       123","isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"author":[{"last_name":"Babic","first_name":"David","id":"db566d23-f6e0-11ea-865d-e6f270e968e7","full_name":"Babic, David"},{"id":"4827E134-F248-11E8-B48F-1D18A9856A87","full_name":"Abualia, Rashed","orcid":"0000-0002-9357-9415","last_name":"Abualia","first_name":"Rashed"},{"id":"7c417475-8972-11ed-ae7b-8b674ca26986","full_name":"Fiedler, Lukas","last_name":"Fiedler","first_name":"Lukas"},{"first_name":"Linlin","last_name":"Qi","full_name":"Qi, Linlin","id":"44B04502-A9ED-11E9-B6FC-583AE6697425","orcid":"0000-0001-5187-8401"},{"last_name":"Tellier","first_name":"Frédérique","full_name":"Tellier, Frédérique"},{"full_name":"Smoljan, Adrijana","id":"cced8a85-223e-11ed-af04-b0596c55053b","first_name":"Adrijana","last_name":"Smoljan"},{"first_name":"Hana","last_name":"Rakusova","full_name":"Rakusova, Hana","id":"4CAAA450-78D2-11EA-8E57-B40A396E08BA"},{"first_name":"Petr","last_name":"Valošek","full_name":"Valošek, Petr","id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","first_name":"Huibin","last_name":"Han"},{"orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","full_name":"Benková, Eva","last_name":"Benková","first_name":"Eva"},{"full_name":"Faure, Jean Denis","first_name":"Jean Denis","last_name":"Faure"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"}],"OA_type":"hybrid","corr_author":"1","date_published":"2025-08-01T00:00:00Z","ddc":["580"],"file":[{"creator":"dernst","content_type":"application/pdf","date_updated":"2025-09-01T14:09:31Z","file_size":5791111,"checksum":"1cdc3341d2d23101abca72521f1f23cb","relation":"main_file","date_created":"2025-09-01T14:09:31Z","access_level":"open_access","success":1,"file_name":"2025_PlantJournal_Babic.pdf","file_id":"20264"}],"year":"2025","PlanS_conform":"1","day":"01","date_updated":"2026-04-07T11:52:02Z","article_type":"original","publisher":"Wiley","pmid":1,"publication_status":"published","status":"public"},{"isi":1,"intvolume":"       115","author":[{"full_name":"Jiang, Lihui","first_name":"Lihui","last_name":"Jiang"},{"last_name":"Yao","first_name":"Baolin","full_name":"Yao, Baolin"},{"full_name":"Zhang, Xiaoyan","first_name":"Xiaoyan","last_name":"Zhang"},{"full_name":"Wu, Lixia","first_name":"Lixia","last_name":"Wu"},{"first_name":"Qijing","last_name":"Fu","full_name":"Fu, Qijing"},{"first_name":"Yiting","last_name":"Zhao","full_name":"Zhao, Yiting"},{"first_name":"Yuxin","last_name":"Cao","full_name":"Cao, Yuxin"},{"last_name":"Zhu","first_name":"Ruomeng","full_name":"Zhu, Ruomeng"},{"first_name":"Xinqi","last_name":"Lu","full_name":"Lu, Xinqi"},{"last_name":"Huang","first_name":"Wuying","full_name":"Huang, Wuying"},{"full_name":"Zhao, Jianping","last_name":"Zhao","first_name":"Jianping"},{"full_name":"Li, Kuixiu","first_name":"Kuixiu","last_name":"Li"},{"full_name":"Zhao, Shuanglu","first_name":"Shuanglu","last_name":"Zhao"},{"last_name":"Han","first_name":"Li","full_name":"Han, Li"},{"full_name":"Zhou, Xuan","last_name":"Zhou","first_name":"Xuan"},{"full_name":"Luo, Chongyu","last_name":"Luo","first_name":"Chongyu"},{"full_name":"Zhu, Haiyan","last_name":"Zhu","first_name":"Haiyan"},{"first_name":"Jing","last_name":"Yang","full_name":"Yang, Jing"},{"full_name":"Huang, Huichuan","last_name":"Huang","first_name":"Huichuan"},{"first_name":"Zhengge","last_name":"Zhu","full_name":"Zhu, Zhengge"},{"first_name":"Xiahong","last_name":"He","full_name":"He, Xiahong"},{"orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","last_name":"Friml","first_name":"Jiří"},{"first_name":"Zhongkai","last_name":"Zhang","full_name":"Zhang, Zhongkai"},{"full_name":"Liu, Changning","last_name":"Liu","first_name":"Changning"},{"last_name":"Du","first_name":"Yunlong","full_name":"Du, Yunlong"}],"date_published":"2023-07-01T00:00:00Z","ddc":["580"],"OA_type":"free access","day":"01","year":"2023","publisher":"Wiley","article_type":"original","date_updated":"2026-06-18T17:29:30Z","status":"public","pmid":1,"publication_status":"published","page":"155-174","external_id":{"pmid":["37025008 "],"isi":["000971861400001"]},"article_processing_charge":"No","title":"Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth","publication":"Plant Journal","abstract":[{"lang":"eng","text":"Salicylic acid (SA) plays important roles in different aspects of plant development, including root growth, where auxin is also a major player by means of its asymmetric distribution. However, the mechanism underlying the effect of SA on the development of rice roots remains poorly understood. Here, we show that SA inhibits rice root growth by interfering with auxin transport associated with the OsPIN3t- and clathrin-mediated gene regulatory network (GRN). SA inhibits root growth as well as Brefeldin A-sensitive trafficking through a non-canonical SA signaling mechanism. Transcriptome analysis of rice seedlings treated with SA revealed that the OsPIN3t auxin transporter is at the center of a GRN involving the coat protein clathrin. The root growth and endocytic trafficking in both the pin3t and clathrin heavy chain mutants were SA insensitivity. SA inhibitory effect on the endocytosis of OsPIN3t was dependent on clathrin; however, the root growth and endocytic trafficking mediated by tyrphostin A23 (TyrA23) were independent of the pin3t mutant under SA treatment. These data reveal that SA affects rice root growth through the convergence of transcriptional and non-SA signaling mechanisms involving OsPIN3t-mediated auxin transport and clathrin-mediated trafficking as key components."}],"volume":115,"department":[{"_id":"JiFr"}],"oa":1,"oa_version":"Published Version","issue":"1","language":[{"iso":"eng"}],"quality_controlled":"1","OA_place":"publisher","scopus_import":"1","citation":{"ista":"Jiang L, Yao B, Zhang X, Wu L, Fu Q, Zhao Y, Cao Y, Zhu R, Lu X, Huang W, Zhao J, Li K, Zhao S, Han L, Zhou X, Luo C, Zhu H, Yang J, Huang H, Zhu Z, He X, Friml J, Zhang Z, Liu C, Du Y. 2023. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. Plant Journal. 115(1), 155–174.","apa":"Jiang, L., Yao, B., Zhang, X., Wu, L., Fu, Q., Zhao, Y., … Du, Y. (2023). Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.16218\">https://doi.org/10.1111/tpj.16218</a>","ieee":"L. Jiang <i>et al.</i>, “Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth,” <i>Plant Journal</i>, vol. 115, no. 1. Wiley, pp. 155–174, 2023.","mla":"Jiang, Lihui, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” <i>Plant Journal</i>, vol. 115, no. 1, Wiley, 2023, pp. 155–74, doi:<a href=\"https://doi.org/10.1111/tpj.16218\">10.1111/tpj.16218</a>.","short":"L. Jiang, B. Yao, X. Zhang, L. Wu, Q. Fu, Y. Zhao, Y. Cao, R. Zhu, X. Lu, W. Huang, J. Zhao, K. Li, S. Zhao, L. Han, X. Zhou, C. Luo, H. Zhu, J. Yang, H. Huang, Z. Zhu, X. He, J. Friml, Z. Zhang, C. Liu, Y. Du, Plant Journal 115 (2023) 155–174.","chicago":"Jiang, Lihui, Baolin Yao, Xiaoyan Zhang, Lixia Wu, Qijing Fu, Yiting Zhao, Yuxin Cao, et al. “Salicylic Acid Inhibits Rice Endocytic Protein Trafficking Mediated by OsPIN3t and Clathrin to Affect Root Growth.” <i>Plant Journal</i>. Wiley, 2023. <a href=\"https://doi.org/10.1111/tpj.16218\">https://doi.org/10.1111/tpj.16218</a>.","ama":"Jiang L, Yao B, Zhang X, et al. Salicylic acid inhibits rice endocytic protein trafficking mediated by OsPIN3t and clathrin to affect root growth. <i>Plant Journal</i>. 2023;115(1):155-174. doi:<a href=\"https://doi.org/10.1111/tpj.16218\">10.1111/tpj.16218</a>"},"month":"07","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","type":"journal_article","date_created":"2023-04-30T22:01:06Z","doi":"10.1111/tpj.16218","publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313X"]},"_id":"12878","acknowledgement":"The authors thank Professor Jianqiang Wu (Kunming Institute of Botany, Chinese Academy of Sciences) for support with phytohormone measurement. Thanks also go to Professor Pieter. B. F. Ouwerkerk (Leiden University) and Professor Jean-Benoit Morel (Plant Health Institute of Montpellier) for provision of the rice lines NB-7B-70 and NB-7B-76 and wild-type NB-61-WT, Professor Zuhua He (Chinese Academy of Sciences) for provision of the rice OsNPR1-RNAi mutant, and Professor Yinong Yang (The Pennsylvania State University) for provision of the rice line NahG. This work was supported by grants from the National Natural Science Foundation of China (Grant Nos. 32260085, 31460453, 31660501, 31860064, 31970609, 31801792 and 31960554), the Key Projects of the Applied Basic Research Plan of Yunnan Province (202301AS070082), the Major Special Program for Scientific Research, Education Department of Yunnan Province (Grant No. ZD2015005), the Start-up fund from Xishuangbanna Tropical Botanical Garden, and ‘Top Talents Program in Science and Technology’ from Yunnan Province, the SRF for ROCS, SEM (Grant No. [2013] 1792), and the Major Science and Technology Project in Yunnan Province (202102AE090042 and 202202AE090036); and the young and middle-aged academic and technical leaders reserve talent program in Yunnan Province (202205AC160076).","main_file_link":[{"url":"https://doi.org/10.1111/tpj.16218","open_access":"1"}]},{"isi":1,"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"intvolume":"        98","has_accepted_license":"1","ddc":["580"],"date_published":"2019-06-01T00:00:00Z","author":[{"full_name":"Rakusová, Hana","last_name":"Rakusová","first_name":"Hana"},{"full_name":"Han, Huibin","id":"31435098-F248-11E8-B48F-1D18A9856A87","first_name":"Huibin","last_name":"Han"},{"full_name":"Valošek, Petr","id":"3CDB6F94-F248-11E8-B48F-1D18A9856A87","first_name":"Petr","last_name":"Valošek"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"}],"day":"01","year":"2019","file":[{"checksum":"ad3b5e270b67ba2a45f894ce3be27920","date_created":"2019-04-15T09:38:43Z","relation":"main_file","access_level":"open_access","creator":"dernst","content_type":"application/pdf","date_updated":"2020-07-14T12:47:25Z","file_size":1383100,"file_name":"2019_PlantJournal_Rakusov.pdf","file_id":"6304"}],"pmid":1,"publication_status":"published","status":"public","article_type":"original","publisher":"Wiley","date_updated":"2025-04-15T07:48:04Z","project":[{"name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","_id":"25716A02-B435-11E9-9278-68D0E5697425","grant_number":"282300"}],"external_id":{"isi":["000473644100008"],"pmid":["30821050"]},"article_processing_charge":"Yes (via OA deal)","page":"1048-1059","file_date_updated":"2020-07-14T12:47:25Z","volume":98,"ec_funded":1,"publication":"The Plant Journal","abstract":[{"text":"Gravitropism is an adaptive response that orients plant growth parallel to the gravity vector. Asymmetric\r\ndistribution of the phytohormone auxin is a necessary prerequisite to the tropic bending both in roots and\r\nshoots. During hypocotyl gravitropic response, the PIN3 auxin transporter polarizes within gravity-sensing\r\ncells to redirect intercellular auxin fluxes. First gravity-induced PIN3 polarization to the bottom cell mem-\r\nbranes leads to the auxin accumulation at the lower side of the organ, initiating bending and, later, auxin\r\nfeedback-mediated repolarization restores symmetric auxin distribution to terminate bending. Here, we per-\r\nformed a forward genetic screen to identify regulators of both PIN3 polarization events during gravitropic\r\nresponse. We searched for mutants with defective PIN3 polarizations based on easy-to-score morphological\r\noutputs of decreased or increased gravity-induced hypocotyl bending. We identified the number of\r\nhypocotyl reduced bending (hrb) and hypocotyl hyperbending (hhb) mutants, revealing that reduced bending corre-\r\nlated typically with defective gravity-induced PIN3 relocation whereas all analyzed hhb mutants showed\r\ndefects in the second, auxin-mediated PIN3 relocation. Next-generation sequencing-aided mutation map-\r\nping identified several candidate genes, including SCARECROW and ACTIN2, revealing roles of endodermis\r\nspecification and actin cytoskeleton in the respective gravity- and auxin-induced PIN polarization events.\r\nThe hypocotyl gravitropism screen thus promises to provide novel insights into mechanisms underlying cell\r\npolarity and plant adaptive development.","lang":"eng"}],"title":"Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls","quality_controlled":"1","language":[{"iso":"eng"}],"issue":"6","department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"publication_identifier":{"issn":["0960-7412"],"eissn":["1365-313x"]},"_id":"6262","doi":"10.1111/tpj.14301","date_created":"2019-04-09T08:46:44Z","citation":{"apa":"Rakusová, H., Han, H., Valošek, P., &#38; Friml, J. (2019). Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. <i>The Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1111/tpj.14301\">https://doi.org/10.1111/tpj.14301</a>","ista":"Rakusová H, Han H, Valošek P, Friml J. 2019. Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. The Plant Journal. 98(6), 1048–1059.","chicago":"Rakusová, Hana, Huibin Han, Petr Valošek, and Jiří Friml. “Genetic Screen for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana Hypocotyls.” <i>The Plant Journal</i>. Wiley, 2019. <a href=\"https://doi.org/10.1111/tpj.14301\">https://doi.org/10.1111/tpj.14301</a>.","ama":"Rakusová H, Han H, Valošek P, Friml J. Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls. <i>The Plant Journal</i>. 2019;98(6):1048-1059. doi:<a href=\"https://doi.org/10.1111/tpj.14301\">10.1111/tpj.14301</a>","ieee":"H. Rakusová, H. Han, P. Valošek, and J. Friml, “Genetic screen for factors mediating PIN polarization in gravistimulated Arabidopsis thaliana hypocotyls,” <i>The Plant Journal</i>, vol. 98, no. 6. Wiley, pp. 1048–1059, 2019.","mla":"Rakusová, Hana, et al. “Genetic Screen for Factors Mediating PIN Polarization in Gravistimulated Arabidopsis Thaliana Hypocotyls.” <i>The Plant Journal</i>, vol. 98, no. 6, Wiley, 2019, pp. 1048–59, doi:<a href=\"https://doi.org/10.1111/tpj.14301\">10.1111/tpj.14301</a>.","short":"H. Rakusová, H. Han, P. Valošek, J. Friml, The Plant Journal 98 (2019) 1048–1059."},"scopus_import":"1","type":"journal_article","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","month":"06"},{"date_updated":"2026-04-16T10:08:30Z","publisher":"Wiley-Blackwell","publication_status":"published","status":"public","year":"2014","day":"01","author":[{"last_name":"Chen","first_name":"Yani","full_name":"Chen, Yani"},{"first_name":"Kyaw","last_name":"Aung","full_name":"Aung, Kyaw"},{"last_name":"Rolčík","first_name":"Jakub","full_name":"Rolčík, Jakub"},{"full_name":"Walicki, Kathryn","last_name":"Walicki","first_name":"Kathryn"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml"},{"full_name":"Brandizzí, Federica","last_name":"Brandizzí","first_name":"Federica"}],"publist_id":"4699","date_published":"2014-01-01T00:00:00Z","intvolume":"        77","isi":1,"type":"journal_article","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","month":"01","citation":{"ama":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. 2014;77(1):97-107. doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>","chicago":"Chen, Yani, Kyaw Aung, Jakub Rolčík, Kathryn Walicki, Jiří Friml, and Federica Brandizzí. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>.","short":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, F. Brandizzí, Plant Journal 77 (2014) 97–107.","mla":"Chen, Yani, et al. “Inter-Regulation of the Unfolded Protein Response and Auxin Signaling.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 97–107, doi:<a href=\"https://doi.org/10.1111/tpj.12373\">10.1111/tpj.12373</a>.","ieee":"Y. Chen, K. Aung, J. Rolčík, K. Walicki, J. Friml, and F. Brandizzí, “Inter-regulation of the unfolded protein response and auxin signaling,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 97–107, 2014.","apa":"Chen, Y., Aung, K., Rolčík, J., Walicki, K., Friml, J., &#38; Brandizzí, F. (2014). Inter-regulation of the unfolded protein response and auxin signaling. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12373\">https://doi.org/10.1111/tpj.12373</a>","ista":"Chen Y, Aung K, Rolčík J, Walicki K, Friml J, Brandizzí F. 2014. Inter-regulation of the unfolded protein response and auxin signaling. Plant Journal. 77(1), 97–107."},"scopus_import":"1","main_file_link":[{"open_access":"1","url":"http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3981873/"}],"publication_identifier":{"issn":["0960-7412"]},"_id":"2249","date_created":"2018-12-11T11:56:34Z","doi":"10.1111/tpj.12373","oa_version":"Submitted Version","oa":1,"department":[{"_id":"JiFr"}],"language":[{"iso":"eng"}],"quality_controlled":"1","issue":"1","title":"Inter-regulation of the unfolded protein response and auxin signaling","volume":77,"publication":"Plant Journal","abstract":[{"lang":"eng","text":"The unfolded protein response (UPR) is a signaling network triggered by overload of protein-folding demand in the endoplasmic reticulum (ER), a condition termed ER stress. The UPR is critical for growth and development; nonetheless, connections between the UPR and other cellular regulatory processes remain largely unknown. Here, we identify a link between the UPR and the phytohormone auxin, a master regulator of plant physiology. We show that ER stress triggers down-regulation of auxin receptors and transporters in Arabidopsis thaliana. We also demonstrate that an Arabidopsis mutant of a conserved ER stress sensor IRE1 exhibits defects in the auxin response and levels. These data not only support that the plant IRE1 is required for auxin homeostasis, they also reveal a species-specific feature of IRE1 in multicellular eukaryotes. Furthermore, by establishing that UPR activation is reduced in mutants of ER-localized auxin transporters, including PIN5, we define a long-neglected biological significance of ER-based auxin regulation. We further examine the functional relationship of IRE1 and PIN5 by showing that an ire1 pin5 triple mutant enhances defects of UPR activation and auxin homeostasis in ire1 or pin5. Our results imply that the plant UPR has evolved a hormone-dependent strategy for coordinating ER function with physiological processes."}],"external_id":{"isi":["000328661300008"]},"page":"97 - 107","article_processing_charge":"No"},{"publisher":"Wiley-Blackwell","article_type":"original","date_updated":"2026-06-18T18:33:25Z","status":"public","publication_status":"published","day":"01","year":"2014","author":[{"full_name":"Bailly, Aurélien","first_name":"Aurélien","last_name":"Bailly"},{"full_name":"Wang, Bangjun","first_name":"Bangjun","last_name":"Wang"},{"full_name":"Zwiewka, Marta","last_name":"Zwiewka","first_name":"Marta"},{"full_name":"Pollmann, Stephan","last_name":"Pollmann","first_name":"Stephan"},{"last_name":"Schenck","first_name":"Daniel","full_name":"Schenck, Daniel"},{"full_name":"Lüthen, Hartwig","first_name":"Hartwig","last_name":"Lüthen"},{"last_name":"Schulz","first_name":"Alexander","full_name":"Schulz, Alexander"},{"first_name":"Jirí","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Geisler, Markus","last_name":"Geisler","first_name":"Markus"}],"publist_id":"4694","date_published":"2014-01-01T00:00:00Z","ddc":["580"],"isi":1,"intvolume":"        77","scopus_import":"1","citation":{"short":"A. Bailly, B. Wang, M. Zwiewka, S. Pollmann, D. Schenck, H. Lüthen, A. Schulz, J. Friml, M. Geisler, Plant Journal 77 (2014) 108–118.","ieee":"A. Bailly <i>et al.</i>, “Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth,” <i>Plant Journal</i>, vol. 77, no. 1. Wiley-Blackwell, pp. 108–118, 2014.","mla":"Bailly, Aurélien, et al. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>, vol. 77, no. 1, Wiley-Blackwell, 2014, pp. 108–18, doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>.","ama":"Bailly A, Wang B, Zwiewka M, et al. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. 2014;77(1):108-118. doi:<a href=\"https://doi.org/10.1111/tpj.12369\">10.1111/tpj.12369</a>","chicago":"Bailly, Aurélien, Bangjun Wang, Marta Zwiewka, Stephan Pollmann, Daniel Schenck, Hartwig Lüthen, Alexander Schulz, Jiří Friml, and Markus Geisler. “Expression of TWISTED DWARF1 Lacking Its In-Plane Membrane Anchor Leads to Increased Cell Elongation and Hypermorphic Growth.” <i>Plant Journal</i>. Wiley-Blackwell, 2014. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>.","ista":"Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M. 2014. Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. Plant Journal. 77(1), 108–118.","apa":"Bailly, A., Wang, B., Zwiewka, M., Pollmann, S., Schenck, D., Lüthen, H., … Geisler, M. (2014). Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth. <i>Plant Journal</i>. Wiley-Blackwell. <a href=\"https://doi.org/10.1111/tpj.12369\">https://doi.org/10.1111/tpj.12369</a>"},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","month":"01","type":"journal_article","doi":"10.1111/tpj.12369","date_created":"2018-12-11T11:56:35Z","_id":"2253","publication_identifier":{"issn":["0960-7412"]},"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1111/tpj.12369"}],"department":[{"_id":"JiFr"}],"oa_version":"Published Version","oa":1,"issue":"1","language":[{"iso":"eng"}],"quality_controlled":"1","title":"Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth","publication":"Plant Journal","abstract":[{"text":"Plant growth is achieved predominantly by cellular elongation, which is thought to be controlled on several levels by apoplastic auxin. Auxin export into the apoplast is achieved by plasma membrane efflux catalysts of the PIN-FORMED (PIN) and ATP-binding cassette protein subfamily B/phosphor- glycoprotein (ABCB/PGP) classes; the latter were shown to depend on interaction with the FKBP42, TWISTED DWARF1 (TWD1). Here by using a transgenic approach in combination with phenotypical, biochemical and cell biological analyses we demonstrate the importance of a putative C-terminal in-plane membrane anchor of TWD1 in the regulation of ABCB-mediated auxin transport. In contrast with dwarfed twd1 loss-of-function alleles, TWD1 gain-of-function lines that lack a putative in-plane membrane anchor (HA-TWD1-Ct) show hypermorphic plant architecture, characterized by enhanced stem length and leaf surface but reduced shoot branching. Greater hypocotyl length is the result of enhanced cell elongation that correlates with reduced polar auxin transport capacity for HA-TWD1-Ct. As a consequence, HA-TWD1-Ct displays higher hypocotyl auxin accumulation, which is shown to result in elevated auxin-induced cell elongation rates. Our data highlight the importance of C-terminal membrane anchoring for TWD1 action, which is required for specific regulation of ABCB-mediated auxin transport. These data support a model in which TWD1 controls lateral ABCB1-mediated export into the apoplast, which is required for auxin-mediated cell elongation.","lang":"eng"}],"volume":77,"project":[{"name":"Innovationsförderung in der Grenzregion Österreich – Tschechische Republik durch die Schaffung von Synergien im Bereich der Forschungsinfrastruktur","_id":"256BDAB0-B435-11E9-9278-68D0E5697425"}],"article_processing_charge":"No","page":"108 - 118","external_id":{"isi":["000328661300009"]}},{"page":"115 - 124","external_id":{"pmid":["12662314"]},"article_processing_charge":"No","title":"Automated whole mount localisation techniques for plant seedlings","abstract":[{"text":"Plant biology is currently experiencing a growing demand for easy and reliable mRNA and protein localisation techniques. Here, we present novel whole mount in situ hybridisation and immunolocalisation protocols, suitable to localise mRNAs and proteins in Arabidopsis seedlings. We demonstrate that these methods can be used in different organs of Arabidopsis seedlings as well as in other plant species. In order to achieve better reproducibility and higher throughput, we modified these protocols for automation to be performed by a liquid handling robot. In addition, we show that other procedures such as reporter enzyme assays and tissue clearing can be similarly automated. We present examples of application of our protocols including mRNA localisation and proteins and epitope tag (co)localisations which demonstrate that these methods provide reliable and versatile tools for expression, localisation and anatomical studies in plants.","lang":"eng"}],"publication":"Plant Journal","volume":34,"oa_version":"None","issue":"1","quality_controlled":"1","language":[{"iso":"eng"}],"scopus_import":"1","citation":{"mla":"Friml, Jiří, et al. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” <i>Plant Journal</i>, vol. 34, no. 1, Wiley, 2003, pp. 115–24, doi:<a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">10.1046/j.1365-313X.2003.01705.x</a>.","ieee":"J. Friml, E. Benková, U. Mayer, K. Palme, and G. Muster, “Automated whole mount localisation techniques for plant seedlings,” <i>Plant Journal</i>, vol. 34, no. 1. Wiley, pp. 115–124, 2003.","short":"J. Friml, E. Benková, U. Mayer, K. Palme, G. Muster, Plant Journal 34 (2003) 115–124.","chicago":"Friml, Jiří, Eva Benková, Ulrike Mayer, Klaus Palme, and Gerhard Muster. “Automated Whole Mount Localisation Techniques for Plant Seedlings.” <i>Plant Journal</i>. Wiley, 2003. <a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">https://doi.org/10.1046/j.1365-313X.2003.01705.x</a>.","ama":"Friml J, Benková E, Mayer U, Palme K, Muster G. Automated whole mount localisation techniques for plant seedlings. <i>Plant Journal</i>. 2003;34(1):115-124. doi:<a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">10.1046/j.1365-313X.2003.01705.x</a>","ista":"Friml J, Benková E, Mayer U, Palme K, Muster G. 2003. Automated whole mount localisation techniques for plant seedlings. Plant Journal. 34(1), 115–124.","apa":"Friml, J., Benková, E., Mayer, U., Palme, K., &#38; Muster, G. (2003). Automated whole mount localisation techniques for plant seedlings. <i>Plant Journal</i>. Wiley. <a href=\"https://doi.org/10.1046/j.1365-313X.2003.01705.x\">https://doi.org/10.1046/j.1365-313X.2003.01705.x</a>"},"month":"04","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","type":"journal_article","date_created":"2018-12-11T12:00:44Z","doi":"10.1046/j.1365-313X.2003.01705.x","_id":"2993","publication_identifier":{"eissn":["1365-313X"],"issn":["0960-7412"]},"extern":"1","intvolume":"        34","author":[{"last_name":"Friml","first_name":"Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí"},{"full_name":"Benková, Eva","id":"38F4F166-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8510-9739","first_name":"Eva","last_name":"Benková"},{"first_name":"Ulrike","last_name":"Mayer","full_name":"Mayer, Ulrike"},{"first_name":"Klaus","last_name":"Palme","full_name":"Palme, Klaus"},{"full_name":"Muster, Gerhard","last_name":"Muster","first_name":"Gerhard"}],"publist_id":"3709","date_published":"2003-04-01T00:00:00Z","OA_type":"closed access","day":"01","year":"2003","article_type":"original","publisher":"Wiley","date_updated":"2026-05-22T09:01:28Z","status":"public","publication_status":"published","pmid":1}]