[{"date_updated":"2026-06-10T08:30:19Z","OA_type":"hybrid","day":"01","has_accepted_license":"1","publication":"The Plant Cell","citation":{"chicago":"Gallei, Michelle C, Sven M Truckenbrodt, Caroline Kreuzinger, Syamala Inumella, Vitali Vistunou, Christoph M Sommer, Mojtaba Tavakoli, et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>. Oxford University Press, 2025. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>.","ista":"Gallei MC, Truckenbrodt SM, Kreuzinger C, Inumella S, Vistunou V, Sommer CM, Tavakoli M, Agudelo Duenas N, Vorlaufer J, Jahr W, Randuch M, Johnson AJ, Benková E, Friml J, Danzl JG. 2025. Super-resolution expansion microscopy in plant roots. The Plant Cell. 37(4), koaf006.","short":"M.C. Gallei, S.M. Truckenbrodt, C. Kreuzinger, S. Inumella, V. Vistunou, C.M. Sommer, M. Tavakoli, N. Agudelo Duenas, J. Vorlaufer, W. Jahr, M. Randuch, A.J. Johnson, E. Benková, J. Friml, J.G. Danzl, The Plant Cell 37 (2025).","ieee":"M. C. Gallei <i>et al.</i>, “Super-resolution expansion microscopy in plant roots,” <i>The Plant Cell</i>, vol. 37, no. 4. Oxford University Press, 2025.","apa":"Gallei, M. C., Truckenbrodt, S. M., Kreuzinger, C., Inumella, S., Vistunou, V., Sommer, C. M., … Danzl, J. G. (2025). Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koaf006\">https://doi.org/10.1093/plcell/koaf006</a>","mla":"Gallei, Michelle C., et al. “Super-Resolution Expansion Microscopy in Plant Roots.” <i>The Plant Cell</i>, vol. 37, no. 4, koaf006, Oxford University Press, 2025, doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>.","ama":"Gallei MC, Truckenbrodt SM, Kreuzinger C, et al. Super-resolution expansion microscopy in plant roots. <i>The Plant Cell</i>. 2025;37(4). doi:<a href=\"https://doi.org/10.1093/plcell/koaf006\">10.1093/plcell/koaf006</a>"},"file":[{"date_updated":"2025-07-31T07:03:43Z","file_name":"2025_PlantCell_Gallei.pdf","file_id":"20092","checksum":"9d3f8218ff37a29f29c48a7bbe831bd3","success":1,"content_type":"application/pdf","file_size":53904111,"creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2025-07-31T07:03:43Z"}],"publisher":"Oxford University Press","department":[{"_id":"EvBe"},{"_id":"JoDa"},{"_id":"JiFr"}],"OA_place":"publisher","title":"Super-resolution expansion microscopy in plant roots","_id":"19003","ddc":["580"],"type":"journal_article","article_processing_charge":"Yes (via OA deal)","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"doi":"10.1093/plcell/koaf006","quality_controlled":"1","ec_funded":1,"status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","scopus_import":"1","related_material":{"record":[{"status":"public","id":"18689","relation":"earlier_version"},{"status":"public","id":"18837","relation":"research_data"}]},"volume":37,"abstract":[{"text":"Super-resolution methods provide far better spatial resolution than the optical diffraction limit of about half the wavelength of light (∼200-300 nm). Nevertheless, they have yet to attain widespread use in plants, largely due to plants’ challenging optical properties. Expansion microscopy improves effective resolution by isotropically increasing the physical distances between sample structures while preserving relative spatial arrangements and clearing the sample. However, its application to plants has been hindered by the rigid, mechanically cohesive structure of plant tissues. Here, we report on whole-mount expansion microscopy of thale cress (Arabidopsis thaliana) root tissues (PlantEx), achieving a four-fold resolution increase over conventional microscopy. Our results highlight the microtubule cytoskeleton organization and interaction between molecularly defined cellular constituents. Combining PlantEx with stimulated emission depletion (STED) microscopy, we increase nanoscale resolution and visualize the complex organization of subcellular organelles from intact tissues by example of the densely packed COPI-coated vesicles associated with the Golgi apparatus and put these into a cellular structural context. Our results show that expansion microscopy can be applied to increase effective imaging resolution in Arabidopsis root specimens. ","lang":"eng"}],"month":"04","acknowledged_ssus":[{"_id":"Bio"},{"_id":"LifeSc"},{"_id":"E-Lib"},{"_id":"M-Shop"}],"language":[{"iso":"eng"}],"acknowledgement":"We gratefully acknowledge support by the Scientific Service Units at ISTA, including the Imaging and Optics and Lab Support facilities and the mechanical workshop and Library. We thank Philipp Velicky for STED microscope alignment.\r\nThis project has received funding from the European Research Council under the Horizon 2020 Framework Programme (grant agreement No 742985, J.F.). It has also received funding from the Horizon 2020 Framework Programme under the Marie Skłodowska-Curie Grant Agreement No. 665385 (M.G.). S.T. has received funding as an ISTplus Fellow from the Horizon 2020 Framework Programme under Marie Skłodowska-Curie grant agreement no. 754411 and from EMBO via a Long-Term Fellowship (grant number ALTF 679-2018). M.R.T. received funding from the Austrian Academy of Sciences with DOC fellowship no. 26137. The project has further received funding from the Austrian Science Fund, via grant DK W1232 (M.R.T., N.A.D., and J.G.D). W.J. received a postdoctoral fellowship from the Human Frontier Science Program (LT000557/2018). The funders had no role in study design, data collection and analysis, decision to publish or preparation of the manuscript.","isi":1,"oa":1,"issue":"4","intvolume":"        37","file_date_updated":"2025-07-31T07:03:43Z","date_published":"2025-04-01T00:00:00Z","year":"2025","article_type":"original","date_created":"2025-02-05T06:52:06Z","author":[{"first_name":"Michelle C","last_name":"Gallei","orcid":"0000-0003-1286-7368","full_name":"Gallei, Michelle C","id":"35A03822-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Truckenbrodt, Sven M","id":"45812BD4-F248-11E8-B48F-1D18A9856A87","first_name":"Sven M","last_name":"Truckenbrodt"},{"first_name":"Caroline","last_name":"Kreuzinger","full_name":"Kreuzinger, Caroline","id":"382077BA-F248-11E8-B48F-1D18A9856A87"},{"orcid":"0009-0002-5890-120X","full_name":"Inumella, Syamala","id":"F8660870-D756-11E9-98C5-34DFE5697425","first_name":"Syamala","last_name":"Inumella"},{"id":"7e146587-8972-11ed-ae7b-d7a32ea86a81","full_name":"Vistunou, Vitali","last_name":"Vistunou","first_name":"Vitali"},{"orcid":"0000-0003-1216-9105","full_name":"Sommer, Christoph M","id":"4DF26D8C-F248-11E8-B48F-1D18A9856A87","first_name":"Christoph M","last_name":"Sommer"},{"orcid":"0000-0002-7667-6854","full_name":"Tavakoli, Mojtaba","id":"3A0A06F4-F248-11E8-B48F-1D18A9856A87","first_name":"Mojtaba","last_name":"Tavakoli"},{"last_name":"Agudelo Duenas","first_name":"Nathalie","id":"40E7F008-F248-11E8-B48F-1D18A9856A87","full_name":"Agudelo Duenas, Nathalie"},{"last_name":"Vorlaufer","first_name":"Jakob","id":"937696FA-C996-11E9-8C7C-CF13E6697425","orcid":"0009-0000-7590-3501","full_name":"Vorlaufer, Jakob"},{"id":"425C1CE8-F248-11E8-B48F-1D18A9856A87","full_name":"Jahr, Wiebke","orcid":"0000-0003-0201-2315","last_name":"Jahr","first_name":"Wiebke"},{"last_name":"Randuch","first_name":"Marek","id":"6ac4636d-15b2-11ec-abd3-fb8df79972ae","full_name":"Randuch, Marek"},{"last_name":"Johnson","first_name":"Alexander J","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843"},{"first_name":"Eva","last_name":"Benková","full_name":"Benková, Eva","orcid":"0000-0002-8510-9739","id":"38F4F166-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"last_name":"Danzl","first_name":"Johann G","id":"42EFD3B6-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-8559-3973","full_name":"Danzl, Johann G"}],"external_id":{"pmid":["39792900"],"isi":["001462763100001"]},"corr_author":"1","publication_status":"published","article_number":"koaf006","tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"pmid":1,"PlanS_conform":"1","project":[{"_id":"261099A6-B435-11E9-9278-68D0E5697425","call_identifier":"H2020","grant_number":"742985","name":"Tracing Evolution of Auxin Transport and Polarity in Plants"},{"_id":"2564DBCA-B435-11E9-9278-68D0E5697425","grant_number":"665385","call_identifier":"H2020","name":"International IST Doctoral Program"},{"_id":"260C2330-B435-11E9-9278-68D0E5697425","name":"ISTplus - Postdoctoral Fellowships","grant_number":"754411","call_identifier":"H2020"},{"_id":"269B5B22-B435-11E9-9278-68D0E5697425","grant_number":"ALTF 679-2018","name":"UltraX - achieving sub-nanometer resolution in light microscopy using iterative X10 microscopy in combination with nanobodies and STED"},{"name":"Studying Organelle Structure and Function at Nanoscale Resolution with Expansion Microscopy","grant_number":"26137","_id":"6285a163-2b32-11ec-9570-8e204ca2dba5"},{"grant_number":"W1232-B24","call_identifier":"FWF","name":"Molecular Drug Targets","_id":"26AA4EF2-B435-11E9-9278-68D0E5697425"}]},{"citation":{"ieee":"L.-Z. Zhou <i>et al.</i>, “The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize,” <i>The Plant Cell</i>, vol. 36, no. 5. Oxford University Press, 2024.","apa":"Zhou, L.-Z., Wang, L., Chen, X., Ge, Z., Mergner, J., Li, X., … Dresselhaus, T. (2024). The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koad324\">https://doi.org/10.1093/plcell/koad324</a>","mla":"Zhou, Liang-Zi, et al. “The RALF Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>, vol. 36, no. 5, koad324, Oxford University Press, 2024, doi:<a href=\"https://doi.org/10.1093/plcell/koad324\">10.1093/plcell/koad324</a>.","ama":"Zhou L-Z, Wang L, Chen X, et al. The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. <i>The Plant Cell</i>. 2024;36(5). doi:<a href=\"https://doi.org/10.1093/plcell/koad324\">10.1093/plcell/koad324</a>","chicago":"Zhou, Liang-Zi, Lele Wang, Xia Chen, Zengxiang Ge, Julia Mergner, Xingli Li, Bernhard Küster, Gernot Längst, Li-Jia Qu, and Thomas Dresselhaus. “The RALF Signaling Pathway Regulates Cell Wall Integrity during Pollen Tube Growth in Maize.” <i>The Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koad324\">https://doi.org/10.1093/plcell/koad324</a>.","ista":"Zhou L-Z, Wang L, Chen X, Ge Z, Mergner J, Li X, Küster B, Längst G, Qu L-J, Dresselhaus T. 2024. The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize. The Plant Cell. 36(5), koad324.","short":"L.-Z. Zhou, L. Wang, X. Chen, Z. Ge, J. Mergner, X. Li, B. Küster, G. Längst, L.-J. Qu, T. Dresselhaus, The Plant Cell 36 (2024)."},"keyword":["Cell Biology","Plant Science"],"has_accepted_license":"1","publication":"The Plant Cell","publisher":"Oxford University Press","main_file_link":[{"open_access":"1","url":"https://doi.org/10.1093/plcell/koad324"}],"_id":"14726","title":"The RALF signaling pathway regulates cell wall integrity during pollen tube growth in maize","date_updated":"2024-07-16T11:18:46Z","day":"01","quality_controlled":"1","doi":"10.1093/plcell/koad324","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"status":"public","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","ddc":["580"],"extern":"1","type":"journal_article","article_processing_charge":"No","oa":1,"language":[{"iso":"eng"}],"issue":"5","intvolume":"        36","year":"2024","article_type":"original","date_published":"2024-05-01T00:00:00Z","date_created":"2024-01-02T11:19:37Z","volume":36,"abstract":[{"lang":"eng","text":"Autocrine signaling pathways regulated by RAPID ALKALINIZATION FACTORs (RALFs) control cell wall integrity during pollen tube germination and growth in Arabidopsis (Arabidopsis thaliana). To investigate the role of pollen-specific RALFs in another plant species, we combined gene expression data with phylogenetic and biochemical studies to identify candidate orthologs in maize (Zea mays). We show that Clade IB ZmRALF2/3 mutations, but not Clade III ZmRALF1/5 mutations, cause cell wall instability in the sub-apical region of the growing pollen tube. ZmRALF2/3 are mainly located in the cell wall and are partially able to complement the pollen germination defect of their Arabidopsis orthologs AtRALF4/19. Mutations in ZmRALF2/3 compromise pectin distribution patterns leading to altered cell wall organization and thickness culminating in pollen tube burst. Clade IB, but not Clade III ZmRALFs, strongly interact as ligands with the pollen-specific Catharanthus roseus RLK1-like (CrRLK1L) receptor kinases Zea mays FERONIA-like (ZmFERL) 4/7/9, LORELEI-like glycosylphosphatidylinositol-anchor (LLG) proteins Zea mays LLG 1 and 2 (ZmLLG1/2) and Zea mays pollen extension-like (PEX) cell wall proteins ZmPEX2/4. Notably, ZmFERL4 outcompetes ZmLLG2 and ZmPEX2 outcompetes ZmFERL4 for ZmRALF2 binding. Based on these data, we suggest that Clade IB RALFs act in a dual role as cell wall components and extracellular sensors to regulate cell wall integrity and thickness during pollen tube growth in maize and probably other plants."}],"month":"05","author":[{"first_name":"Liang-Zi","last_name":"Zhou","full_name":"Zhou, Liang-Zi"},{"last_name":"Wang","first_name":"Lele","full_name":"Wang, Lele"},{"full_name":"Chen, Xia","first_name":"Xia","last_name":"Chen"},{"last_name":"Ge","first_name":"Zengxiang","id":"f43371a3-09ff-11eb-8013-bd0c6a2f6de8","orcid":"0000-0001-9381-3577","full_name":"Ge, Zengxiang"},{"full_name":"Mergner, Julia","last_name":"Mergner","first_name":"Julia"},{"full_name":"Li, Xingli","first_name":"Xingli","last_name":"Li"},{"full_name":"Küster, Bernhard","first_name":"Bernhard","last_name":"Küster"},{"full_name":"Längst, Gernot","first_name":"Gernot","last_name":"Längst"},{"first_name":"Li-Jia","last_name":"Qu","full_name":"Qu, Li-Jia"},{"first_name":"Thomas","last_name":"Dresselhaus","full_name":"Dresselhaus, Thomas"}],"external_id":{"pmid":["38142229"]},"tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"article_number":"koad324","pmid":1,"publication_status":"published"},{"external_id":{"pmid":["38124479"]},"publication_status":"published","tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"pmid":1,"author":[{"full_name":"Huebbers, Jan W.","first_name":"Jan W.","last_name":"Huebbers"},{"last_name":"Caldarescu","first_name":"George A.","full_name":"Caldarescu, George A."},{"full_name":"Kubátová, Zdeňka","first_name":"Zdeňka","last_name":"Kubátová"},{"last_name":"Sabol","first_name":"Peter","full_name":"Sabol, Peter"},{"last_name":"Levecque","first_name":"Sophie C.J.","full_name":"Levecque, Sophie C.J."},{"last_name":"Kuhn","first_name":"Hannah","full_name":"Kuhn, Hannah"},{"first_name":"Ivan","last_name":"Kulich","full_name":"Kulich, Ivan","id":"57a1567c-8314-11eb-9063-c9ddc3451a54"},{"full_name":"Reinstädler, Anja","first_name":"Anja","last_name":"Reinstädler"},{"full_name":"Büttgen, Kim","first_name":"Kim","last_name":"Büttgen"},{"full_name":"Manga-Robles, Alba","first_name":"Alba","last_name":"Manga-Robles"},{"last_name":"Mélida","first_name":"Hugo","full_name":"Mélida, Hugo"},{"last_name":"Pauly","first_name":"Markus","full_name":"Pauly, Markus"},{"last_name":"Panstruga","first_name":"Ralph","full_name":"Panstruga, Ralph"},{"first_name":"Viktor","last_name":"Žárský","full_name":"Žárský, Viktor"}],"abstract":[{"text":"Exocyst component of 70-kDa (EXO70) proteins are constituents of the exocyst complex implicated in vesicle tethering during exocytosis. MILDEW RESISTANCE LOCUS O (MLO) proteins are plant-specific calcium channels and some MLO isoforms enable fungal powdery mildew pathogenesis. We here detected an unexpected phenotypic overlap of Arabidopsis thaliana exo70H4 and mlo2 mlo6 mlo12 triple mutant plants regarding the biogenesis of leaf trichome secondary cell walls. Biochemical and Fourier transform infrared spectroscopic analyses corroborated deficiencies in the composition of trichome cell walls in these mutants. Transgenic lines expressing fluorophore-tagged EXO70H4 and MLO exhibited extensive colocalization of these proteins. Furthermore, mCherry-EXO70H4 mislocalized in trichomes of the mlo triple mutant and, vice versa, MLO6-GFP mislocalized in trichomes of the exo70H4 mutant. Expression of GFP-marked PMR4 callose synthase, a known cargo of EXO70H4-dependent exocytosis, revealed reduced cell wall delivery of GFP-PMR4 in trichomes of mlo triple mutant plants. In vivo protein–protein interaction assays in plant and yeast cells uncovered isoform-preferential interactions between EXO70.2 subfamily members and MLO proteins. Finally, exo70H4 and mlo6 mutants, when combined, showed synergistically enhanced resistance to powdery mildew attack. Taken together, our data point to an isoform-specific interplay of EXO70 and MLO proteins in the modulation of trichome cell wall biogenesis and powdery mildew susceptibility.","lang":"eng"}],"month":"04","volume":36,"file_date_updated":"2024-04-17T06:57:54Z","date_published":"2024-04-01T00:00:00Z","article_type":"original","year":"2024","date_created":"2024-04-14T22:01:02Z","oa":1,"language":[{"iso":"eng"}],"issue":"4","intvolume":"        36","type":"journal_article","article_processing_charge":"Yes (in subscription journal)","ddc":["580"],"oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","page":"1007-1035","doi":"10.1093/plcell/koad319","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"quality_controlled":"1","status":"public","day":"01","date_updated":"2024-04-17T07:01:21Z","title":"Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew","_id":"15319","has_accepted_license":"1","publication":"Plant Cell","citation":{"ama":"Huebbers JW, Caldarescu GA, Kubátová Z, et al. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. <i>Plant Cell</i>. 2024;36(4):1007-1035. doi:<a href=\"https://doi.org/10.1093/plcell/koad319\">10.1093/plcell/koad319</a>","mla":"Huebbers, Jan W., et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” <i>Plant Cell</i>, vol. 36, no. 4, Oxford University Press, 2024, pp. 1007–35, doi:<a href=\"https://doi.org/10.1093/plcell/koad319\">10.1093/plcell/koad319</a>.","apa":"Huebbers, J. W., Caldarescu, G. A., Kubátová, Z., Sabol, P., Levecque, S. C. J., Kuhn, H., … Žárský, V. (2024). Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. <i>Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koad319\">https://doi.org/10.1093/plcell/koad319</a>","ieee":"J. W. Huebbers <i>et al.</i>, “Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew,” <i>Plant Cell</i>, vol. 36, no. 4. Oxford University Press, pp. 1007–1035, 2024.","short":"J.W. Huebbers, G.A. Caldarescu, Z. Kubátová, P. Sabol, S.C.J. Levecque, H. Kuhn, I. Kulich, A. Reinstädler, K. Büttgen, A. Manga-Robles, H. Mélida, M. Pauly, R. Panstruga, V. Žárský, Plant Cell 36 (2024) 1007–1035.","ista":"Huebbers JW, Caldarescu GA, Kubátová Z, Sabol P, Levecque SCJ, Kuhn H, Kulich I, Reinstädler A, Büttgen K, Manga-Robles A, Mélida H, Pauly M, Panstruga R, Žárský V. 2024. Interplay of EXO70 and MLO proteins modulates trichome cell wall composition and susceptibility to powdery mildew. Plant Cell. 36(4), 1007–1035.","chicago":"Huebbers, Jan W., George A. Caldarescu, Zdeňka Kubátová, Peter Sabol, Sophie C.J. Levecque, Hannah Kuhn, Ivan Kulich, et al. “Interplay of EXO70 and MLO Proteins Modulates Trichome Cell Wall Composition and Susceptibility to Powdery Mildew.” <i>Plant Cell</i>. Oxford University Press, 2024. <a href=\"https://doi.org/10.1093/plcell/koad319\">https://doi.org/10.1093/plcell/koad319</a>."},"file":[{"creator":"dernst","content_type":"application/pdf","file_size":2866098,"relation":"main_file","date_created":"2024-04-17T06:57:54Z","access_level":"open_access","file_name":"2024_PlantCell_Huebbers.pdf","date_updated":"2024-04-17T06:57:54Z","success":1,"file_id":"15326","checksum":"f9994d2e4748feec24c992b39871aba1"}],"department":[{"_id":"JiFr"}],"publisher":"Oxford University Press"},{"volume":35,"month":"06","abstract":[{"text":"The study of RNAs has become one of the most influential research fields in contemporary biology and biomedicine. In the last few years, new sequencing technologies have produced an explosion of new and exciting discoveries in the field but have also given rise to many open questions. Defining these questions, together with old, long-standing gaps in our knowledge, is the spirit of this article. The breadth of topics within RNA biology research is vast, and every aspect of the biology of these molecules contains countless exciting open questions. Here, we asked 12 groups to discuss their most compelling question among some plant RNA biology topics. The following vignettes cover RNA alternative splicing; RNA dynamics; RNA translation; RNA structures; R-loops; epitranscriptomics; long non-coding RNAs; small RNA production and their functions in crops; small RNAs during gametogenesis and in cross-kingdom RNA interference; and RNA-directed DNA methylation. In each section, we will present the current state-of-the-art in plant RNA biology research before asking the questions that will surely motivate future discoveries in the field. We hope this article will spark a debate about the future perspective on RNA biology and provoke novel reflections in the reader.","lang":"eng"}],"intvolume":"        35","language":[{"iso":"eng"}],"oa":1,"issue":"6","date_created":"2023-02-23T09:14:59Z","article_type":"original","year":"2023","date_published":"2023-06-01T00:00:00Z","author":[{"full_name":"Manavella, Pablo A","last_name":"Manavella","first_name":"Pablo A"},{"first_name":"Micaela A","last_name":"Godoy Herz","full_name":"Godoy Herz, Micaela A"},{"last_name":"Kornblihtt","first_name":"Alberto R","full_name":"Kornblihtt, Alberto R"},{"first_name":"Reed","last_name":"Sorenson","full_name":"Sorenson, Reed"},{"first_name":"Leslie E","last_name":"Sieburth","full_name":"Sieburth, Leslie E"},{"last_name":"Nakaminami","first_name":"Kentaro","full_name":"Nakaminami, Kentaro"},{"first_name":"Motoaki","last_name":"Seki","full_name":"Seki, Motoaki"},{"first_name":"Yiliang","last_name":"Ding","full_name":"Ding, Yiliang"},{"first_name":"Qianwen","last_name":"Sun","full_name":"Sun, Qianwen"},{"last_name":"Kang","first_name":"Hunseung","full_name":"Kang, Hunseung"},{"last_name":"Ariel","first_name":"Federico D","full_name":"Ariel, Federico D"},{"full_name":"Crespi, Martin","last_name":"Crespi","first_name":"Martin"},{"full_name":"Giudicatti, Axel J","first_name":"Axel J","last_name":"Giudicatti"},{"full_name":"Cai, Qiang","first_name":"Qiang","last_name":"Cai"},{"first_name":"Hailing","last_name":"Jin","full_name":"Jin, Hailing"},{"orcid":"0000-0002-4008-1234","full_name":"Feng, Xiaoqi","id":"e0164712-22ee-11ed-b12a-d80fcdf35958","first_name":"Xiaoqi","last_name":"Feng"},{"first_name":"Yijun","last_name":"Qi","full_name":"Qi, Yijun"},{"full_name":"Pikaard, Craig S","first_name":"Craig S","last_name":"Pikaard"}],"pmid":1,"article_number":"koac346","publication_status":"published","external_id":{"pmid":["36477566"]},"date_updated":"2023-10-04T09:48:43Z","day":"01","publisher":"Oxford University Press","department":[{"_id":"XiFe"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1093/plcell/koac346"}],"citation":{"mla":"Manavella, Pablo A., et al. “Beyond Transcription: Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>, vol. 35, no. 6, koac346, Oxford University Press, 2023, doi:<a href=\"https://doi.org/10.1093/plcell/koac346\">10.1093/plcell/koac346</a>.","apa":"Manavella, P. A., Godoy Herz, M. A., Kornblihtt, A. R., Sorenson, R., Sieburth, L. E., Nakaminami, K., … Pikaard, C. S. (2023). Beyond transcription: compelling open questions in plant RNA biology. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koac346\">https://doi.org/10.1093/plcell/koac346</a>","ieee":"P. A. Manavella <i>et al.</i>, “Beyond transcription: compelling open questions in plant RNA biology,” <i>The Plant Cell</i>, vol. 35, no. 6. Oxford University Press, 2023.","ama":"Manavella PA, Godoy Herz MA, Kornblihtt AR, et al. Beyond transcription: compelling open questions in plant RNA biology. <i>The Plant Cell</i>. 2023;35(6). doi:<a href=\"https://doi.org/10.1093/plcell/koac346\">10.1093/plcell/koac346</a>","ista":"Manavella PA, Godoy Herz MA, Kornblihtt AR, Sorenson R, Sieburth LE, Nakaminami K, Seki M, Ding Y, Sun Q, Kang H, Ariel FD, Crespi M, Giudicatti AJ, Cai Q, Jin H, Feng X, Qi Y, Pikaard CS. 2023. Beyond transcription: compelling open questions in plant RNA biology. The Plant Cell. 35(6), koac346.","chicago":"Manavella, Pablo A, Micaela A Godoy Herz, Alberto R Kornblihtt, Reed Sorenson, Leslie E Sieburth, Kentaro Nakaminami, Motoaki Seki, et al. “Beyond Transcription: Compelling Open Questions in Plant RNA Biology.” <i>The Plant Cell</i>. Oxford University Press, 2023. <a href=\"https://doi.org/10.1093/plcell/koac346\">https://doi.org/10.1093/plcell/koac346</a>.","short":"P.A. Manavella, M.A. Godoy Herz, A.R. Kornblihtt, R. Sorenson, L.E. Sieburth, K. Nakaminami, M. Seki, Y. Ding, Q. Sun, H. Kang, F.D. Ariel, M. Crespi, A.J. Giudicatti, Q. Cai, H. Jin, X. Feng, Y. Qi, C.S. Pikaard, The Plant Cell 35 (2023)."},"keyword":["Cell Biology","Plant Science"],"publication":"The Plant Cell","_id":"12669","title":"Beyond transcription: compelling open questions in plant RNA biology","article_processing_charge":"No","extern":"1","type":"journal_article","status":"public","quality_controlled":"1","doi":"10.1093/plcell/koac346","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","oa_version":"Published Version"},{"project":[{"name":"Molecular mechanisms of endocytic cargo recognition in plants","call_identifier":"FWF","grant_number":"I03630","_id":"26538374-B435-11E9-9278-68D0E5697425"}],"author":[{"first_name":"DA","last_name":"Dahhan","full_name":"Dahhan, DA"},{"first_name":"GD","last_name":"Reynolds","full_name":"Reynolds, GD"},{"last_name":"Cárdenas","first_name":"JJ","full_name":"Cárdenas, JJ"},{"full_name":"Eeckhout, D","first_name":"D","last_name":"Eeckhout"},{"full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson"},{"first_name":"K","last_name":"Yperman","full_name":"Yperman, K"},{"orcid":"0000-0001-9735-5315","full_name":"Kaufmann, Walter","id":"3F99E422-F248-11E8-B48F-1D18A9856A87","first_name":"Walter","last_name":"Kaufmann"},{"full_name":"Vang, N","first_name":"N","last_name":"Vang"},{"last_name":"Yan","first_name":"X","full_name":"Yan, X"},{"full_name":"Hwang, I","last_name":"Hwang","first_name":"I"},{"first_name":"A","last_name":"Heese","full_name":"Heese, A"},{"full_name":"De Jaeger, G","last_name":"De Jaeger","first_name":"G"},{"first_name":"Jiří","last_name":"Friml","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87"},{"full_name":"Van Damme, D","last_name":"Van Damme","first_name":"D"},{"first_name":"J","last_name":"Pan","full_name":"Pan, J"},{"first_name":"SY","last_name":"Bednarek","full_name":"Bednarek, SY"}],"publication_status":"published","pmid":1,"external_id":{"isi":["000767438800001"],"pmid":["35218346"]},"intvolume":"        34","language":[{"iso":"eng"}],"acknowledgement":"The authors would like to acknowledge the VIB Proteomics Core Facility (VIB-UGent Center for Medical Biotechnology in Ghent, Belgium) and the Research Technology Support Facility Proteomics Core (Michigan State University in East Lansing, Michigan) for sample analysis, as well as the University of Wisconsin Biotechnology Center Mass Spectrometry Core Facility (Madison, WI) for help with data processing. Additionally, we are grateful to Sue Weintraub (UT Health San Antonio) and Sydney Thomas (UW- Madison) for assistance with data analysis. This research was supported by grants to S.Y.B. from the National Science Foundation (Nos. 1121998 and 1614915) and a Vilas Associate Award (University of Wisconsin, Madison, Graduate School); to J.P. from the National Natural Science Foundation of China (Nos. 91754104, 31820103008, and 31670283); to I.H. from the National Research Foundation of Korea (No. 2019R1A2B5B03099982). This research was also supported by the Scientific Service Units (SSU) of IST Austria through resources provided by the Electron microscopy Facility (EMF). A.J. is supported by funding from the Austrian Science Fund (FWF): I3630B25 to J.F. A.H. is supported by funding from the National Science Foundation (NSF IOS Nos. 1025837 and 1147032).","oa":1,"issue":"6","isi":1,"date_created":"2022-03-08T13:47:51Z","date_published":"2022-06-01T00:00:00Z","year":"2022","article_type":"original","volume":34,"month":"06","acknowledged_ssus":[{"_id":"EM-Fac"}],"abstract":[{"lang":"eng","text":"In eukaryotes, clathrin-coated vesicles (CCVs) facilitate the internalization of material from the cell surface as well as the movement of cargo in post-Golgi trafficking pathways. This diversity of functions is partially provided by multiple monomeric and multimeric clathrin adaptor complexes that provide compartment and cargo selectivity. The adaptor-protein assembly polypeptide-1 (AP-1) complex operates as part of the secretory pathway at the trans-Golgi network (TGN), while the AP-2 complex and the TPLATE complex jointly operate at the plasma membrane to execute clathrin-mediated endocytosis. Key to our further understanding of clathrin-mediated trafficking in plants will be the comprehensive identification and characterization of the network of evolutionarily conserved and plant-specific core and accessory machinery involved in the formation and targeting of CCVs. To facilitate these studies, we have analyzed the proteome of enriched TGN/early endosome-derived and endocytic CCVs isolated from dividing and expanding suspension-cultured Arabidopsis (Arabidopsis thaliana) cells. Tandem mass spectrometry analysis results were validated by differential chemical labeling experiments to identify proteins co-enriching with CCVs. Proteins enriched in CCVs included previously characterized CCV components and cargos such as the vacuolar sorting receptors in addition to conserved and plant-specific components whose function in clathrin-mediated trafficking has not been previously defined. Notably, in addition to AP-1 and AP-2, all subunits of the AP-4 complex, but not AP-3 or AP-5, were found to be in high abundance in the CCV proteome. The association of AP-4 with suspension-cultured Arabidopsis CCVs is further supported via additional biochemical data."}],"status":"public","doi":"10.1093/plcell/koac071","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"quality_controlled":"1","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Preprint","page":"2150-2173","article_processing_charge":"No","type":"journal_article","main_file_link":[{"url":"https://doi.org/10.1101/2021.09.16.460678","open_access":"1"}],"publisher":"Oxford University Press","department":[{"_id":"JiFr"},{"_id":"EM-Fac"}],"publication":"Plant Cell","citation":{"short":"D. Dahhan, G. Reynolds, J. Cárdenas, D. Eeckhout, A.J. Johnson, K. Yperman, W. Kaufmann, N. Vang, X. Yan, I. Hwang, A. Heese, G. De Jaeger, J. Friml, D. Van Damme, J. Pan, S. Bednarek, Plant Cell 34 (2022) 2150–2173.","ista":"Dahhan D, Reynolds G, Cárdenas J, Eeckhout D, Johnson AJ, Yperman K, Kaufmann W, Vang N, Yan X, Hwang I, Heese A, De Jaeger G, Friml J, Van Damme D, Pan J, Bednarek S. 2022. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. Plant Cell. 34(6), 2150–2173.","chicago":"Dahhan, DA, GD Reynolds, JJ Cárdenas, D Eeckhout, Alexander J Johnson, K Yperman, Walter Kaufmann, et al. “Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.” <i>Plant Cell</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/plcell/koac071\">https://doi.org/10.1093/plcell/koac071</a>.","ama":"Dahhan D, Reynolds G, Cárdenas J, et al. Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. <i>Plant Cell</i>. 2022;34(6):2150-2173. doi:<a href=\"https://doi.org/10.1093/plcell/koac071\">10.1093/plcell/koac071</a>","apa":"Dahhan, D., Reynolds, G., Cárdenas, J., Eeckhout, D., Johnson, A. J., Yperman, K., … Bednarek, S. (2022). Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components. <i>Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koac071\">https://doi.org/10.1093/plcell/koac071</a>","mla":"Dahhan, DA, et al. “Proteomic Characterization of Isolated Arabidopsis Clathrin-Coated Vesicles Reveals Evolutionarily Conserved and Plant-Specific Components.” <i>Plant Cell</i>, vol. 34, no. 6, Oxford University Press, 2022, pp. 2150–73, doi:<a href=\"https://doi.org/10.1093/plcell/koac071\">10.1093/plcell/koac071</a>.","ieee":"D. Dahhan <i>et al.</i>, “Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components,” <i>Plant Cell</i>, vol. 34, no. 6. Oxford University Press, pp. 2150–2173, 2022."},"title":"Proteomic characterization of isolated Arabidopsis clathrin-coated vesicles reveals evolutionarily conserved and plant-specific components","_id":"10841","date_updated":"2025-05-14T11:06:15Z","day":"01"},{"title":"Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum)","_id":"12053","file":[{"access_level":"open_access","relation":"main_file","date_created":"2023-01-20T08:29:12Z","content_type":"application/pdf","file_size":3282540,"creator":"dernst","checksum":"1c606d9545f29dfca15235f69ad27b58","file_id":"12318","success":1,"date_updated":"2023-01-20T08:29:12Z","file_name":"2022_PlantCell_Tian.pdf"}],"publisher":"Oxford University Press","department":[{"_id":"JiFr"}],"publication":"The Plant Cell","has_accepted_license":"1","citation":{"short":"Z. Tian, Y. Zhang, L. Zhu, B. Jiang, H. Wang, R. Gao, J. Friml, G. Xiao, The Plant Cell 34 (2022) 4816–4839.","chicago":"Tian, Z, Yuzhou Zhang, L Zhu, B Jiang, H Wang, R Gao, Jiří Friml, and G Xiao. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” <i>The Plant Cell</i>. Oxford University Press, 2022. <a href=\"https://doi.org/10.1093/plcell/koac270\">https://doi.org/10.1093/plcell/koac270</a>.","ista":"Tian Z, Zhang Y, Zhu L, Jiang B, Wang H, Gao R, Friml J, Xiao G. 2022. Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). The Plant Cell. 34(12), 4816–4839.","ama":"Tian Z, Zhang Y, Zhu L, et al. Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). <i>The Plant Cell</i>. 2022;34(12):4816-4839. doi:<a href=\"https://doi.org/10.1093/plcell/koac270\">10.1093/plcell/koac270</a>","ieee":"Z. Tian <i>et al.</i>, “Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum),” <i>The Plant Cell</i>, vol. 34, no. 12. Oxford University Press, pp. 4816–4839, 2022.","mla":"Tian, Z., et al. “Strigolactones Act Downstream of Gibberellins to Regulate Fiber Cell Elongation and Cell Wall Thickness in Cotton (Gossypium Hirsutum).” <i>The Plant Cell</i>, vol. 34, no. 12, Oxford University Press, 2022, pp. 4816–39, doi:<a href=\"https://doi.org/10.1093/plcell/koac270\">10.1093/plcell/koac270</a>.","apa":"Tian, Z., Zhang, Y., Zhu, L., Jiang, B., Wang, H., Gao, R., … Xiao, G. (2022). Strigolactones act downstream of gibberellins to regulate fiber cell elongation and cell wall thickness in cotton (Gossypium hirsutum). <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/plcell/koac270\">https://doi.org/10.1093/plcell/koac270</a>"},"day":"01","date_updated":"2025-04-15T08:12:07Z","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","scopus_import":"1","oa_version":"Published Version","page":"4816-4839","related_material":{"link":[{"url":"https://doi.org/10.1093/plcell/koac342","relation":"erratum"}]},"status":"public","doi":"10.1093/plcell/koac270","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"quality_controlled":"1","article_processing_charge":"No","type":"journal_article","ddc":["580"],"date_created":"2022-09-07T14:19:39Z","file_date_updated":"2023-01-20T08:29:12Z","date_published":"2022-12-01T00:00:00Z","year":"2022","article_type":"original","intvolume":"        34","language":[{"iso":"eng"}],"oa":1,"issue":"12","isi":1,"acknowledgement":"This work was supported by the National Natural Science Foundation of China (32070549), Shaanxi Youth Entrusted Talent Program (20190205), Fundamental Research Funds for the Central Universities (GK202002005 and GK202201017), Young Elite Scientists Sponsorship Program by China Association for Science and Technology (CAST) (2019-2021QNRC001), State Key Laboratory of Cotton Biology Open Fund (CB2020A12 and CB2021A21) and FWF Stand-alone Project (P29988).","month":"12","abstract":[{"lang":"eng","text":"Strigolactones (SLs) are a class of phytohormones that regulate plant shoot branching and adventitious root development. However, little is known regarding the role of SLs in controlling the behavior of the smallest unit of the organism, the single cell. Here, taking advantage of a classic single-cell model offered by the cotton (Gossypium hirsutum) fiber cell, we show that SLs, whose biosynthesis is fine-tuned by gibberellins (GAs), positively regulate cell elongation and cell wall thickness by promoting the biosynthesis of very-long-chain fatty acids (VLCFAs) and cellulose, respectively. Furthermore, we identified two layers of transcription factors (TFs) involved in the hierarchical regulation of this GA-SL crosstalk. The top-layer TF GROWTH-REGULATING FACTOR 4 (GhGRF4) directly activates expression of the SL biosynthetic gene DWARF27 (D27) to increase SL accumulation in fiber cells and GAs induce GhGRF4 expression. SLs induce the expression of four second-layer TF genes (GhNAC100-2, GhBLH51, GhGT2, and GhB9SHZ1), which transmit SL signals downstream to two ketoacyl-CoA synthase genes (KCS) and three cellulose synthase (CesA) genes by directly activating their transcription. Finally, the KCS and CesA enzymes catalyze the biosynthesis of very long chain fatty acids and cellulose, respectively, to regulate development of high-grade cotton fibers. In addition to providing a theoretical basis for cotton fiber improvement, our results shed light on SL signaling in plant development at the single-cell level."}],"volume":34,"project":[{"_id":"262EF96E-B435-11E9-9278-68D0E5697425","grant_number":"P29988","call_identifier":"FWF","name":"RNA-directed DNA methylation in plant development"}],"publication_status":"published","tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"pmid":1,"external_id":{"pmid":["36040191"],"isi":["000852753000001"]},"author":[{"last_name":"Tian","first_name":"Z","full_name":"Tian, Z"},{"id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","full_name":"Zhang, Yuzhou","orcid":"0000-0003-2627-6956","last_name":"Zhang","first_name":"Yuzhou"},{"full_name":"Zhu, L","last_name":"Zhu","first_name":"L"},{"last_name":"Jiang","first_name":"B","full_name":"Jiang, B"},{"first_name":"H","last_name":"Wang","full_name":"Wang, H"},{"last_name":"Gao","first_name":"R","full_name":"Gao, R"},{"full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jiří","last_name":"Friml"},{"first_name":"G","last_name":"Xiao","full_name":"Xiao, G"}]},{"citation":{"ama":"Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, et al. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. <i>Plant Cell</i>. 2021;33(7):2431-2453. doi:<a href=\"https://doi.org/10.1093/plcell/koab122\">10.1093/plcell/koab122</a>","apa":"Ruiz-Lopez, N., Pérez-Sancho, J., Esteban Del Valle, A., Haslam, R., Vanneste, S., Catalá, R., … Botella, M. (2021). Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plcell/koab122\">https://doi.org/10.1093/plcell/koab122</a>","mla":"Ruiz-Lopez, N., et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.” <i>Plant Cell</i>, vol. 33, no. 7, American Society of Plant Biologists, 2021, pp. 2431–53, doi:<a href=\"https://doi.org/10.1093/plcell/koab122\">10.1093/plcell/koab122</a>.","ieee":"N. Ruiz-Lopez <i>et al.</i>, “Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress,” <i>Plant Cell</i>, vol. 33, no. 7. American Society of Plant Biologists, pp. 2431–2453, 2021.","short":"N. Ruiz-Lopez, J. Pérez-Sancho, A. Esteban Del Valle, R. Haslam, S. Vanneste, R. Catalá, C. Perea-Resa, D. Van Damme, S. García-Hernández, A. Albert, J. Vallarino, J. Lin, J. Friml, A. Macho, J. Salinas, A. Rosado, J. Napier, V. Amorim-Silva, M. Botella, Plant Cell 33 (2021) 2431–2453.","ista":"Ruiz-Lopez N, Pérez-Sancho J, Esteban Del Valle A, Haslam R, Vanneste S, Catalá R, Perea-Resa C, Van Damme D, García-Hernández S, Albert A, Vallarino J, Lin J, Friml J, Macho A, Salinas J, Rosado A, Napier J, Amorim-Silva V, Botella M. 2021. Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress. Plant Cell. 33(7), 2431–2453.","chicago":"Ruiz-Lopez, N, J Pérez-Sancho, A Esteban Del Valle, RP Haslam, S Vanneste, R Catalá, C Perea-Resa, et al. “Synaptotagmins at the Endoplasmic Reticulum-Plasma Membrane Contact Sites Maintain Diacylglycerol Homeostasis during Abiotic Stress.” <i>Plant Cell</i>. American Society of Plant Biologists, 2021. <a href=\"https://doi.org/10.1093/plcell/koab122\">https://doi.org/10.1093/plcell/koab122</a>."},"has_accepted_license":"1","publication":"Plant Cell","publisher":"American Society of Plant Biologists","department":[{"_id":"JiFr"}],"file":[{"checksum":"22d596678d00310d793611864a6d0fcd","file_id":"10141","success":1,"date_updated":"2021-10-14T13:36:38Z","file_name":"2021_PlantCell_RuizLopez.pdf","access_level":"open_access","relation":"main_file","date_created":"2021-10-14T13:36:38Z","file_size":2952028,"content_type":"application/pdf","creator":"cchlebak"}],"_id":"9443","title":"Synaptotagmins at the endoplasmic reticulum-plasma membrane contact sites maintain diacylglycerol homeostasis during abiotic stress","date_updated":"2025-04-14T07:45:00Z","day":"01","quality_controlled":"1","doi":"10.1093/plcell/koab122","publication_identifier":{"eissn":["1532-298x"],"issn":["1040-4651"]},"status":"public","ec_funded":1,"page":"2431-2453","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","scopus_import":"1","ddc":["580"],"type":"journal_article","article_processing_charge":"No","isi":1,"issue":"7","acknowledgement":"We would also like to thank Lothar Willmitzer for the lipidomic analysis at the Max Planck Institute of Molecular Plant Physiology (Potsdam, Germany). We thank Manuela Vega from SCI for her technical assistance in image analysis. We thank John R. Pearson and the Bionand Nanoimaging Unit, F. David Navas Fernández and the SCAI Imaging Facility and The Plant Cell Biology facility at the Shanghai Center for Plant Stress Biology for assistance with confocal microscopy. The FaFAH1 clone was a gift from Iraida Amaya Saavedra (IFAPA-Centro de Churriana, Málaga, Spain). The AHA3 antibody against the H+-ATPase was a gift from Ramón Serrano Salom (Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain). The MAP-mTU2-SAC1 construct was provided by Yvon Jaillais (Laboratoire Reproduction et Développement des Plantes, Univ Lyon, France). The pGWB5 from the pGWB vector series, was provided by Tsuyoshi Nakagawa (Department of Molecular and Functional Genomics, Shimane University). We thank Plan Propio from the University of Málaga for financial support.\r\nFunding","oa":1,"language":[{"iso":"eng"}],"intvolume":"        33","article_type":"original","year":"2021","file_date_updated":"2021-10-14T13:36:38Z","date_published":"2021-07-01T00:00:00Z","date_created":"2021-06-02T13:13:58Z","volume":33,"abstract":[{"text":"Endoplasmic reticulum–plasma membrane contact sites (ER–PM CS) play fundamental roles in all eukaryotic cells. Arabidopsis thaliana mutants lacking the ER–PM protein tether synaptotagmin1 (SYT1) exhibit decreased PM integrity under multiple abiotic stresses, such as freezing, high salt, osmotic stress, and mechanical damage. Here, we show that, together with SYT1, the stress-induced SYT3 is an ER–PM tether that also functions in maintaining PM integrity. The ER–PM CS localization of SYT1 and SYT3 is dependent on PM phosphatidylinositol-4-phosphate and is regulated by abiotic stress. Lipidomic analysis revealed that cold stress increased the accumulation of diacylglycerol at the PM in a syt1/3 double mutant relative to wild-type while the levels of most glycerolipid species remain unchanged. In addition, the SYT1-green fluorescent protein fusion preferentially binds diacylglycerol in vivo with little affinity for polar glycerolipids. Our work uncovers a SYT-dependent mechanism of stress adaptation counteracting the detrimental accumulation of diacylglycerol at the PM produced during episodes of abiotic stress.","lang":"eng"}],"month":"07","project":[{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","call_identifier":"H2020","grant_number":"742985","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"author":[{"full_name":"Ruiz-Lopez, N","first_name":"N","last_name":"Ruiz-Lopez"},{"last_name":"Pérez-Sancho","first_name":"J","full_name":"Pérez-Sancho, J"},{"full_name":"Esteban Del Valle, A","last_name":"Esteban Del Valle","first_name":"A"},{"last_name":"Haslam","first_name":"RP","full_name":"Haslam, RP"},{"full_name":"Vanneste, S","last_name":"Vanneste","first_name":"S"},{"full_name":"Catalá, R","last_name":"Catalá","first_name":"R"},{"last_name":"Perea-Resa","first_name":"C","full_name":"Perea-Resa, C"},{"first_name":"D","last_name":"Van Damme","full_name":"Van Damme, D"},{"full_name":"García-Hernández, S","last_name":"García-Hernández","first_name":"S"},{"full_name":"Albert, A","first_name":"A","last_name":"Albert"},{"full_name":"Vallarino, J","first_name":"J","last_name":"Vallarino"},{"full_name":"Lin, J","last_name":"Lin","first_name":"J"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596","last_name":"Friml","first_name":"Jiří"},{"full_name":"Macho, AP","first_name":"AP","last_name":"Macho"},{"last_name":"Salinas","first_name":"J","full_name":"Salinas, J"},{"first_name":"A","last_name":"Rosado","full_name":"Rosado, A"},{"first_name":"JA","last_name":"Napier","full_name":"Napier, JA"},{"last_name":"Amorim-Silva","first_name":"V","full_name":"Amorim-Silva, V"},{"first_name":"MA","last_name":"Botella","full_name":"Botella, MA"}],"external_id":{"pmid":["33944955"],"isi":["000703938100026"]},"tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"pmid":1,"publication_status":"published"},{"author":[{"full_name":"Gao, Z","first_name":"Z","last_name":"Gao"},{"first_name":"Z","last_name":"Chen","full_name":"Chen, Z"},{"last_name":"Cui","first_name":"Y","full_name":"Cui, Y"},{"full_name":"Ke, M","first_name":"M","last_name":"Ke"},{"last_name":"Xu","first_name":"H","full_name":"Xu, H"},{"full_name":"Xu, Q","first_name":"Q","last_name":"Xu"},{"full_name":"Chen, J","last_name":"Chen","first_name":"J"},{"first_name":"Y","last_name":"Li","full_name":"Li, Y"},{"first_name":"L","last_name":"Huang","full_name":"Huang, L"},{"full_name":"Zhao, H","last_name":"Zhao","first_name":"H"},{"full_name":"Huang, D","last_name":"Huang","first_name":"D"},{"last_name":"Mai","first_name":"S","full_name":"Mai, S"},{"first_name":"T","last_name":"Xu","full_name":"Xu, T"},{"full_name":"Liu, X","last_name":"Liu","first_name":"X"},{"first_name":"S","last_name":"Li","full_name":"Li, S"},{"full_name":"Guan, Y","last_name":"Guan","first_name":"Y"},{"full_name":"Yang, W","last_name":"Yang","first_name":"W"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"last_name":"Petrášek","first_name":"J","full_name":"Petrášek, J"},{"full_name":"Zhang, J","last_name":"Zhang","first_name":"J"},{"first_name":"X","last_name":"Chen","full_name":"Chen, X"}],"publication_status":"published","pmid":1,"tmp":{"image":"/images/cc_by_nc_nd.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode","name":"Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)","short":"CC BY-NC-ND (4.0)"},"external_id":{"pmid":["34240197"],"isi":["000702165300012"]},"intvolume":"        33","issue":"9","isi":1,"oa":1,"language":[{"iso":"eng"}],"date_created":"2021-07-14T15:32:43Z","date_published":"2021-07-07T00:00:00Z","file_date_updated":"2021-07-19T12:13:34Z","article_type":"original","year":"2021","volume":33,"month":"07","abstract":[{"text":"To overcome nitrogen deficiency, legume roots establish symbiotic interactions with nitrogen-fixing rhizobia that is fostered in specialized organs (nodules). Similar to other organs, nodule formation is determined by a local maximum of the phytohormone auxin at the primordium site. However, how auxin regulates nodule development remains poorly understood. Here, we found that in soybean, (Glycine max), dynamic auxin transport driven by PIN-FORMED (PIN) transporter GmPIN1 is involved in nodule primordium formation. GmPIN1 was specifically expressed in nodule primordium cells and GmPIN1 was polarly localized in these cells. Two nodulation regulators, (iso)flavonoids trigger expanded distribution of GmPIN1b to root cortical cells, and cytokinin rearranges GmPIN1b polarity. Gmpin1abc triple mutants generated with CRISPR-Cas9 showed impaired establishment of auxin maxima in nodule meristems and aberrant divisions in the nodule primordium cells. Moreover, overexpression of GmPIN1 suppressed nodule primordium initiation. GmPIN9d, an ortholog of Arabidopsis thaliana PIN2, acts together with GmPIN1 later in nodule development to acropetally transport auxin in vascular bundles, fine-tuning the auxin supply for nodule enlargement. Our findings reveal how PIN-dependent auxin transport modulates different aspects of soybean nodule development and suggest that establishment of auxin gradient is a prerequisite for the proper interaction between legumes and rhizobia.","lang":"eng"}],"status":"public","doi":"10.1093/plcell/koab183","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"quality_controlled":"1","scopus_import":"1","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","oa_version":"Published Version","page":"2981–3003","ddc":["580"],"article_processing_charge":"No","type":"journal_article","file":[{"content_type":"application/pdf","file_size":10566921,"creator":"cziletti","access_level":"open_access","date_created":"2021-07-19T12:13:34Z","relation":"main_file","date_updated":"2021-07-19T12:13:34Z","file_name":"2021_PlantCell_Gao.pdf","checksum":"6715712ec306c321f0204c817b7f8ae7","file_id":"9691","success":1}],"department":[{"_id":"JiFr"}],"publisher":"American Society of Plant Biologists","has_accepted_license":"1","publication":"Plant Cell","citation":{"chicago":"Gao, Z, Z Chen, Y Cui, M Ke, H Xu, Q Xu, J Chen, et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean Nodule Development.” <i>Plant Cell</i>. American Society of Plant Biologists, 2021. <a href=\"https://doi.org/10.1093/plcell/koab183\">https://doi.org/10.1093/plcell/koab183</a>.","ista":"Gao Z, Chen Z, Cui Y, Ke M, Xu H, Xu Q, Chen J, Li Y, Huang L, Zhao H, Huang D, Mai S, Xu T, Liu X, Li S, Guan Y, Yang W, Friml J, Petrášek J, Zhang J, Chen X. 2021. GmPIN-dependent polar auxin transport is involved in soybean nodule development. Plant Cell. 33(9), 2981–3003.","short":"Z. Gao, Z. Chen, Y. Cui, M. Ke, H. Xu, Q. Xu, J. Chen, Y. Li, L. Huang, H. Zhao, D. Huang, S. Mai, T. Xu, X. Liu, S. Li, Y. Guan, W. Yang, J. Friml, J. Petrášek, J. Zhang, X. Chen, Plant Cell 33 (2021) 2981–3003.","ieee":"Z. Gao <i>et al.</i>, “GmPIN-dependent polar auxin transport is involved in soybean nodule development,” <i>Plant Cell</i>, vol. 33, no. 9. American Society of Plant Biologists, pp. 2981–3003, 2021.","apa":"Gao, Z., Chen, Z., Cui, Y., Ke, M., Xu, H., Xu, Q., … Chen, X. (2021). GmPIN-dependent polar auxin transport is involved in soybean nodule development. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1093/plcell/koab183\">https://doi.org/10.1093/plcell/koab183</a>","mla":"Gao, Z., et al. “GmPIN-Dependent Polar Auxin Transport Is Involved in Soybean Nodule Development.” <i>Plant Cell</i>, vol. 33, no. 9, American Society of Plant Biologists, 2021, pp. 2981–3003, doi:<a href=\"https://doi.org/10.1093/plcell/koab183\">10.1093/plcell/koab183</a>.","ama":"Gao Z, Chen Z, Cui Y, et al. GmPIN-dependent polar auxin transport is involved in soybean nodule development. <i>Plant Cell</i>. 2021;33(9):2981–3003. doi:<a href=\"https://doi.org/10.1093/plcell/koab183\">10.1093/plcell/koab183</a>"},"_id":"9657","title":"GmPIN-dependent polar auxin transport is involved in soybean nodule development","date_updated":"2024-10-21T06:02:03Z","day":"07"},{"project":[{"grant_number":"742985","call_identifier":"H2020","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","_id":"261099A6-B435-11E9-9278-68D0E5697425"},{"_id":"26538374-B435-11E9-9278-68D0E5697425","grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants"}],"pmid":1,"publication_status":"published","corr_author":"1","external_id":{"pmid":["32193204"],"isi":["000545741500030"]},"author":[{"last_name":"Zhang","first_name":"Xixi","id":"61A66458-47E9-11EA-85BA-8AEAAF14E49A","full_name":"Zhang, Xixi","orcid":"0000-0001-7048-4627"},{"last_name":"Adamowski","first_name":"Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek"},{"id":"44E59624-F248-11E8-B48F-1D18A9856A87","full_name":"Marhavá, Petra","last_name":"Marhavá","first_name":"Petra"},{"id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285","last_name":"Tan","first_name":"Shutang"},{"last_name":"Zhang","first_name":"Yuzhou","id":"3B6137F2-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0003-2627-6956","full_name":"Zhang, Yuzhou"},{"last_name":"Rodriguez Solovey","first_name":"Lesia","id":"3922B506-F248-11E8-B48F-1D18A9856A87","full_name":"Rodriguez Solovey, Lesia","orcid":"0000-0002-7244-7237"},{"full_name":"Zwiewka, Marta","last_name":"Zwiewka","first_name":"Marta"},{"full_name":"Pukyšová, Vendula","last_name":"Pukyšová","first_name":"Vendula"},{"full_name":"Sánchez, Adrià Sans","last_name":"Sánchez","first_name":"Adrià Sans"},{"full_name":"Raxwal, Vivek Kumar","first_name":"Vivek Kumar","last_name":"Raxwal"},{"first_name":"Christian S.","last_name":"Hardtke","full_name":"Hardtke, Christian S."},{"full_name":"Nodzynski, Tomasz","last_name":"Nodzynski","first_name":"Tomasz"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jiří"}],"date_created":"2020-03-28T07:39:22Z","year":"2020","article_type":"original","date_published":"2020-05-01T00:00:00Z","intvolume":"        32","language":[{"iso":"eng"}],"oa":1,"issue":"5","isi":1,"acknowledged_ssus":[{"_id":"Bio"}],"month":"05","abstract":[{"lang":"eng","text":"Cell polarity is a fundamental feature of all multicellular organisms. In plants, prominent cell polarity markers are PIN auxin transporters crucial for plant development. To identify novel components involved in cell polarity establishment and maintenance, we carried out a forward genetic screening with PIN2:PIN1-HA;pin2 Arabidopsis plants, which ectopically express predominantly basally localized PIN1 in the root epidermal cells leading to agravitropic root growth. From the screen, we identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused PIN1-HA polarity switch from basal to apical side of root epidermal cells. Complementation experiments established the repp12 causative mutation as an amino acid substitution in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase with predicted function in vesicle formation. ala3 T-DNA mutants show defects in many auxin-regulated processes, in asymmetric auxin distribution and in PIN trafficking. Analysis of quintuple and sextuple mutants confirmed a crucial role of ALA proteins in regulating plant development and in PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with GNOM and BIG3 ARF GEFs. Taken together, our results identified ALA3 flippase as an important interactor and regulator of ARF GEF functioning in PIN polarity, trafficking and auxin-mediated development."}],"volume":32,"page":"1644-1664","oa_version":"Published Version","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","scopus_import":"1","status":"public","ec_funded":1,"quality_controlled":"1","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"doi":"10.1105/tpc.19.00869","article_processing_charge":"No","type":"journal_article","ddc":["580"],"_id":"7619","title":"Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters","publisher":"American Society of Plant Biologists","department":[{"_id":"JiFr"}],"main_file_link":[{"url":"https://doi.org/10.1105/tpc.19.00869","open_access":"1"}],"citation":{"ista":"Zhang X, Adamowski M, Marhavá P, Tan S, Zhang Y, Rodriguez Solovey L, Zwiewka M, Pukyšová V, Sánchez AS, Raxwal VK, Hardtke CS, Nodzynski T, Friml J. 2020. Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. The Plant Cell. 32(5), 1644–1664.","chicago":"Zhang, Xixi, Maciek Adamowski, Petra Marhavá, Shutang Tan, Yuzhou Zhang, Lesia Rodriguez Solovey, Marta Zwiewka, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.19.00869\">https://doi.org/10.1105/tpc.19.00869</a>.","short":"X. Zhang, M. Adamowski, P. Marhavá, S. Tan, Y. Zhang, L. Rodriguez Solovey, M. Zwiewka, V. Pukyšová, A.S. Sánchez, V.K. Raxwal, C.S. Hardtke, T. Nodzynski, J. Friml, The Plant Cell 32 (2020) 1644–1664.","mla":"Zhang, Xixi, et al. “Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters.” <i>The Plant Cell</i>, vol. 32, no. 5, American Society of Plant Biologists, 2020, pp. 1644–64, doi:<a href=\"https://doi.org/10.1105/tpc.19.00869\">10.1105/tpc.19.00869</a>.","apa":"Zhang, X., Adamowski, M., Marhavá, P., Tan, S., Zhang, Y., Rodriguez Solovey, L., … Friml, J. (2020). Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.19.00869\">https://doi.org/10.1105/tpc.19.00869</a>","ieee":"X. Zhang <i>et al.</i>, “Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters,” <i>The Plant Cell</i>, vol. 32, no. 5. American Society of Plant Biologists, pp. 1644–1664, 2020.","ama":"Zhang X, Adamowski M, Marhavá P, et al. Arabidopsis flippases cooperate with ARF GTPase exchange factors to regulate the trafficking and polarity of PIN auxin transporters. <i>The Plant Cell</i>. 2020;32(5):1644-1664. doi:<a href=\"https://doi.org/10.1105/tpc.19.00869\">10.1105/tpc.19.00869</a>"},"publication":"The Plant Cell","day":"01","date_updated":"2026-06-18T19:24:19Z"},{"intvolume":"        32","isi":1,"language":[{"iso":"eng"}],"issue":"11","oa":1,"date_created":"2020-10-05T12:45:16Z","date_published":"2020-11-01T00:00:00Z","year":"2020","article_type":"original","volume":32,"month":"11","abstract":[{"text":"Clathrin-mediated endocytosis (CME) and its core endocytic machinery are evolutionarily conserved across all eukaryotes. In mammals, the heterotetrameric adaptor protein complex-2 (AP-2) sorts plasma membrane (PM) cargoes into vesicles through the recognition of motifs based on tyrosine or di-leucine in their cytoplasmic tails. However, in plants, very little is known on how PM proteins are sorted for CME and whether similar motifs are required. In Arabidopsis thaliana, the brassinosteroid (BR) receptor, BR INSENSITIVE1 (BRI1), undergoes endocytosis that depends on clathrin and AP-2. Here we demonstrate that BRI1 binds directly to the medium AP-2 subunit, AP2M. The cytoplasmic domain of BRI1 contains five putative canonical surface-exposed tyrosine-based endocytic motifs. The tyrosine-to-phenylalanine substitution in Y898KAI reduced BRI1 internalization without affecting its kinase activity. Consistently, plants carrying the BRI1Y898F mutation were hypersensitive to BRs. Our study demonstrates that AP-2-dependent internalization of PM proteins via the recognition of functional tyrosine motifs also operates in plants.","lang":"eng"}],"project":[{"grant_number":"I03630","call_identifier":"FWF","name":"Molecular mechanisms of endocytic cargo recognition in plants","_id":"26538374-B435-11E9-9278-68D0E5697425"},{"_id":"261099A6-B435-11E9-9278-68D0E5697425","name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","call_identifier":"H2020"}],"author":[{"full_name":"Liu, D","last_name":"Liu","first_name":"D"},{"last_name":"Kumar","first_name":"R","full_name":"Kumar, R"},{"last_name":"LAN","first_name":"Claus","full_name":"LAN, Claus"},{"full_name":"Johnson, Alexander J","orcid":"0000-0002-2739-8843","id":"46A62C3A-F248-11E8-B48F-1D18A9856A87","first_name":"Alexander J","last_name":"Johnson"},{"full_name":"Siao, W","first_name":"W","last_name":"Siao"},{"last_name":"Vanhoutte","first_name":"I","full_name":"Vanhoutte, I"},{"first_name":"P","last_name":"Wang","full_name":"Wang, P"},{"first_name":"KW","last_name":"Bender","full_name":"Bender, KW"},{"full_name":"Yperman, K","last_name":"Yperman","first_name":"K"},{"last_name":"Martins","first_name":"S","full_name":"Martins, S"},{"first_name":"X","last_name":"Zhao","full_name":"Zhao, X"},{"full_name":"Vert, G","last_name":"Vert","first_name":"G"},{"first_name":"D","last_name":"Van Damme","full_name":"Van Damme, D"},{"last_name":"Friml","first_name":"Jiří","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jiří","orcid":"0000-0002-8302-7596"},{"last_name":"Russinova","first_name":"E","full_name":"Russinova, E"}],"publication_status":"published","pmid":1,"external_id":{"pmid":["32958564"],"isi":["000600226800021"]},"main_file_link":[{"url":"https://europepmc.org/article/MED/32958564","open_access":"1"}],"department":[{"_id":"JiFr"}],"publisher":"American Society of Plant Biologists","publication":"Plant Cell","citation":{"mla":"Liu, D., et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>, vol. 32, no. 11, American Society of Plant Biologists, 2020, pp. 3598–612, doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>.","apa":"Liu, D., Kumar, R., LAN, C., Johnson, A. J., Siao, W., Vanhoutte, I., … Russinova, E. (2020). Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>","ieee":"D. Liu <i>et al.</i>, “Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif,” <i>Plant Cell</i>, vol. 32, no. 11. American Society of Plant Biologists, pp. 3598–3612, 2020.","ama":"Liu D, Kumar R, LAN C, et al. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. <i>Plant Cell</i>. 2020;32(11):3598-3612. doi:<a href=\"https://doi.org/10.1105/tpc.20.00384\">10.1105/tpc.20.00384</a>","ista":"Liu D, Kumar R, LAN C, Johnson AJ, Siao W, Vanhoutte I, Wang P, Bender K, Yperman K, Martins S, Zhao X, Vert G, Van Damme D, Friml J, Russinova E. 2020. Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif. Plant Cell. 32(11), 3598–3612.","chicago":"Liu, D, R Kumar, Claus LAN, Alexander J Johnson, W Siao, I Vanhoutte, P Wang, et al. “Endocytosis of BRASSINOSTEROID INSENSITIVE1 Is Partly Driven by a Canonical Tyrosine-Based Motif.” <i>Plant Cell</i>. American Society of Plant Biologists, 2020. <a href=\"https://doi.org/10.1105/tpc.20.00384\">https://doi.org/10.1105/tpc.20.00384</a>.","short":"D. Liu, R. Kumar, C. LAN, A.J. Johnson, W. Siao, I. Vanhoutte, P. Wang, K. Bender, K. Yperman, S. Martins, X. Zhao, G. Vert, D. Van Damme, J. Friml, E. Russinova, Plant Cell 32 (2020) 3598–3612."},"_id":"8607","title":"Endocytosis of BRASSINOSTEROID INSENSITIVE1 is partly driven by a canonical tyrosine-based Motif","date_updated":"2026-06-18T19:35:07Z","day":"01","status":"public","ec_funded":1,"publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298x"]},"doi":"10.1105/tpc.20.00384","quality_controlled":"1","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"Published Version","page":"3598-3612","ddc":["580"],"article_processing_charge":"No","type":"journal_article"},{"pmid":1,"publication_status":"published","corr_author":"1","external_id":{"pmid":["30018156"],"isi":["000450000500023"]},"author":[{"last_name":"Kania","first_name":"Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","full_name":"Kania, Urszula"},{"first_name":"Tomasz","last_name":"Nodzyński","full_name":"Nodzyński, Tomasz"},{"full_name":"Lu, Qing","first_name":"Qing","last_name":"Lu"},{"full_name":"Hicks, Glenn R","first_name":"Glenn R","last_name":"Hicks"},{"first_name":"Wim","last_name":"Nerinckx","full_name":"Nerinckx, Wim"},{"first_name":"Kiril","last_name":"Mishev","full_name":"Mishev, Kiril"},{"last_name":"Peurois","first_name":"Francois","full_name":"Peurois, Francois"},{"full_name":"Cherfils, Jacqueline","last_name":"Cherfils","first_name":"Jacqueline"},{"full_name":"De, Rycke Riet Maria","last_name":"De","first_name":"Rycke Riet Maria"},{"full_name":"Grones, Peter","id":"399876EC-F248-11E8-B48F-1D18A9856A87","first_name":"Peter","last_name":"Grones"},{"full_name":"Robert, Stéphanie","last_name":"Robert","first_name":"Stéphanie"},{"full_name":"Russinova, Eugenia","first_name":"Eugenia","last_name":"Russinova"},{"id":"4159519E-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","last_name":"Friml","first_name":"Jirí"}],"project":[{"call_identifier":"FP7","grant_number":"282300","name":"Polarity and subcellular dynamics in plants","_id":"25716A02-B435-11E9-9278-68D0E5697425"},{"name":"Tracing Evolution of Auxin Transport and Polarity in Plants","grant_number":"742985","call_identifier":"H2020","_id":"261099A6-B435-11E9-9278-68D0E5697425"}],"month":"11","abstract":[{"text":"The trafficking of subcellular cargos in eukaryotic cells crucially depends on vesicle budding, a process mediated by ARF-GEFs (ADP-ribosylation factor guanine nucleotide exchange factors). In plants, ARF-GEFs play essential roles in endocytosis, vacuolar trafficking, recycling, secretion, and polar trafficking. Moreover, they are important for plant development, mainly through controlling the polar subcellular localization of PIN-FORMED (PIN) transporters of the plant hormone auxin. Here, using a chemical genetics screen in Arabidopsis thaliana, we identified Endosidin 4 (ES4), an inhibitor of eukaryotic ARF-GEFs. ES4 acts similarly to and synergistically with the established ARF-GEF inhibitor Brefeldin A and has broad effects on intracellular trafficking, including endocytosis, exocytosis, and vacuolar targeting. Additionally, Arabidopsis and yeast (Sacharomyces cerevisiae) mutants defective in ARF-GEF show altered sensitivity to ES4. ES4 interferes with the activation-based membrane association of the ARF1 GTPases, but not of their mutant variants that are activated independently of ARF-GEF activity. Biochemical approaches and docking simulations confirmed that ES4 specifically targets the SEC7 domain-containing ARF-GEFs. These observations collectively identify ES4 as a chemical tool enabling the study of ARF-GEF-mediated processes, including ARF-GEF-mediated plant development.","lang":"eng"}],"volume":30,"date_created":"2018-12-11T11:44:52Z","publist_id":"7776","article_type":"original","year":"2018","date_published":"2018-11-12T00:00:00Z","intvolume":"        30","isi":1,"language":[{"iso":"eng"}],"issue":"10","oa":1,"acknowledgement":"We thank Gerd Jürgens, Sandra Richter, and Sheng Yang He for providing antibodies; Maciek Adamowski, Fernando Aniento, Sebastian Bednarek, Nico Callewaert, Matyás Fendrych, Elena Feraru, and Mugurel I. Feraru for helpful suggestions; Siamsa Doyle for critical reading of the manuscript and helpful comments and suggestions; and Stephanie Smith and Martine De Cock for help in editing and language corrections. We acknowledge the core facility Cellular Imaging of CEITEC supported by the Czech-BioImaging large RI project (LM2015062 funded by MEYS CR) for their support with obtaining scientific data presented in this article. Plant Sciences Core Facility of CEITEC Masaryk University is gratefully acknowledged for obtaining part of the scientific data presented in this article. We acknowledge support from the Fondation pour la Recherche Médicale and from the Institut National du Cancer (J.C.). The research leading to these results was funded by the European Research Council under the European Union's 7th Framework Program (FP7/2007-2013)/ERC grant agreement numbers 282300 and 742985 and the Czech Science Foundation GAČR (GA18-26981S; J.F.); Ministry of Education, Youth, and Sports/MEYS of the Czech Republic under the Project CEITEC 2020 (LQ1601; T.N.); the China Science Council for a predoctoral fellowship (Q.L.); a joint research project within the framework of cooperation between the Research Foundation-Flanders and the Bulgarian Academy of Sciences (VS.025.13N; K.M. and E.R.); Vetenskapsrådet and Vinnova (Verket för Innovationssystem; S.R.), Knut och Alice Wallenbergs Stiftelse via “Shapesystem” Grant 2012.0050 (S.R.), Kempe stiftelserna (P.G.), Tryggers CTS410 (P.G.).","article_processing_charge":"No","type":"journal_article","ddc":["580"],"page":"2553 - 2572","oa_version":"Published Version","scopus_import":"1","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","ec_funded":1,"status":"public","quality_controlled":"1","publication_identifier":{"issn":["1040-4651"]},"doi":"10.1105/tpc.18.00127","day":"12","date_updated":"2026-06-18T17:36:26Z","title":"The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes","_id":"147","publisher":"Oxford University Press","department":[{"_id":"JiFr"}],"main_file_link":[{"open_access":"1","url":"https://doi.org/10.1105/tpc.18.00127"}],"citation":{"ieee":"U. Kania <i>et al.</i>, “The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes,” <i>The Plant Cell</i>, vol. 30, no. 10. Oxford University Press, pp. 2553–2572, 2018.","apa":"Kania, U., Nodzyński, T., Lu, Q., Hicks, G. R., Nerinckx, W., Mishev, K., … Friml, J. (2018). The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes. <i>The Plant Cell</i>. Oxford University Press. <a href=\"https://doi.org/10.1105/tpc.18.00127\">https://doi.org/10.1105/tpc.18.00127</a>","mla":"Kania, Urszula, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.” <i>The Plant Cell</i>, vol. 30, no. 10, Oxford University Press, 2018, pp. 2553–72, doi:<a href=\"https://doi.org/10.1105/tpc.18.00127\">10.1105/tpc.18.00127</a>.","ama":"Kania U, Nodzyński T, Lu Q, et al. The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes. <i>The Plant Cell</i>. 2018;30(10):2553-2572. doi:<a href=\"https://doi.org/10.1105/tpc.18.00127\">10.1105/tpc.18.00127</a>","chicago":"Kania, Urszula, Tomasz Nodzyński, Qing Lu, Glenn R Hicks, Wim Nerinckx, Kiril Mishev, Francois Peurois, et al. “The Inhibitor Endosidin 4 Targets SEC7 Domain-Type ARF GTPase Exchange Factors and Interferes with Sub Cellular Trafficking in Eukaryotes.” <i>The Plant Cell</i>. Oxford University Press, 2018. <a href=\"https://doi.org/10.1105/tpc.18.00127\">https://doi.org/10.1105/tpc.18.00127</a>.","ista":"Kania U, Nodzyński T, Lu Q, Hicks GR, Nerinckx W, Mishev K, Peurois F, Cherfils J, De RRM, Grones P, Robert S, Russinova E, Friml J. 2018. The inhibitor Endosidin 4 targets SEC7 domain-type ARF GTPase exchange factors and interferes with sub cellular trafficking in eukaryotes. The Plant Cell. 30(10), 2553–2572.","short":"U. Kania, T. Nodzyński, Q. Lu, G.R. Hicks, W. Nerinckx, K. Mishev, F. Peurois, J. Cherfils, R.R.M. De, P. Grones, S. Robert, E. Russinova, J. Friml, The Plant Cell 30 (2018) 2553–2572."},"publication":"The Plant Cell"},{"isi":1,"language":[{"iso":"eng"}],"acknowledgement":"We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9 construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek, Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych, Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for help with correcting the manuscript. This work was supported by the European Research Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project NPUI-LO1417.","oa":1,"issue":"3","intvolume":"        30","article_type":"original","publist_id":"7417","year":"2018","file_date_updated":"2022-05-23T09:12:38Z","date_published":"2018-04-09T00:00:00Z","date_created":"2018-12-11T11:46:20Z","volume":30,"abstract":[{"lang":"eng","text":"Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which cargoes and lipids are internalized from the plasma membrane into vesicles coated with clathrin and adaptor proteins. CME is essential for many developmental and physiological processes in plants, but its underlying mechanism is not well characterised compared to that in yeast and animal systems. Here, we searched for new factors involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification of proteins that interact with clathrin light chain, a principal component of the clathrin coat. Among the confirmed interactors, we found two putative homologues of the clathrin-coat uncoating factor auxilin previously described in non-plant systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused an arrest of seedling growth and development. This was concomitant with inhibited endocytosis due to blocking of clathrin recruitment after the initial step of adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2) loss-of-function lines did not present endocytosis-related developmental or cellular phenotypes under normal growth conditions. This work contributes to the on-going characterization of the endocytotic machinery in plants and provides a robust tool for conditionally and specifically interfering with CME in A. thaliana."}],"month":"04","project":[{"_id":"25716A02-B435-11E9-9278-68D0E5697425","name":"Polarity and subcellular dynamics in plants","call_identifier":"FP7","grant_number":"282300"}],"author":[{"orcid":"0000-0001-6463-5257","full_name":"Adamowski, Maciek","id":"45F536D2-F248-11E8-B48F-1D18A9856A87","first_name":"Maciek","last_name":"Adamowski"},{"last_name":"Narasimhan","first_name":"Madhumitha","id":"44BF24D0-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-8600-0671","full_name":"Narasimhan, Madhumitha"},{"last_name":"Kania","first_name":"Urszula","id":"4AE5C486-F248-11E8-B48F-1D18A9856A87","full_name":"Kania, Urszula"},{"full_name":"Glanc, Matous","orcid":"0000-0003-0619-7783","id":"1AE1EA24-02D0-11E9-9BAA-DAF4881429F2","first_name":"Matous","last_name":"Glanc"},{"full_name":"De Jaeger, Geert","last_name":"De Jaeger","first_name":"Geert"},{"last_name":"Friml","first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"corr_author":"1","external_id":{"isi":["000429441400018"],"pmid":["29511054"]},"pmid":1,"tmp":{"image":"/images/cc_by.png","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)"},"publication_status":"published","citation":{"ama":"Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. <i>The Plant Cell</i>. 2018;30(3):700-716. doi:<a href=\"https://doi.org/10.1105/tpc.17.00785\">10.1105/tpc.17.00785</a>","apa":"Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., &#38; Friml, J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.17.00785\">https://doi.org/10.1105/tpc.17.00785</a>","mla":"Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>, vol. 30, no. 3, American Society of Plant Biologists, 2018, pp. 700–16, doi:<a href=\"https://doi.org/10.1105/tpc.17.00785\">10.1105/tpc.17.00785</a>.","ieee":"M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml, “A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis,” <i>The Plant Cell</i>, vol. 30, no. 3. American Society of Plant Biologists, pp. 700–716, 2018.","short":"M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml, The Plant Cell 30 (2018) 700–716.","ista":"Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis. The Plant Cell. 30(3), 700–716.","chicago":"Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc, Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2018. <a href=\"https://doi.org/10.1105/tpc.17.00785\">https://doi.org/10.1105/tpc.17.00785</a>."},"publication":"The Plant Cell","has_accepted_license":"1","publisher":"American Society of Plant Biologists","department":[{"_id":"JiFr"}],"file":[{"file_id":"11406","checksum":"4e165e653b67d3f0684697f21aace5a1","success":1,"date_updated":"2022-05-23T09:12:38Z","file_name":"2018_PlantCell_Adamowski.pdf","access_level":"open_access","relation":"main_file","date_created":"2022-05-23T09:12:38Z","file_size":4407538,"content_type":"application/pdf","creator":"dernst"}],"title":"A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis","_id":"412","date_updated":"2026-06-28T22:30:33Z","day":"09","quality_controlled":"1","doi":"10.1105/tpc.17.00785","publication_identifier":{"issn":["1040-4651"],"eissn":["1532-298X"]},"status":"public","ec_funded":1,"related_material":{"record":[{"relation":"dissertation_contains","id":"6269","status":"public"}]},"page":"700 - 716","scopus_import":"1","oa_version":"Published Version","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","ddc":["580"],"type":"journal_article","article_processing_charge":"No"},{"type":"journal_article","extern":"1","article_processing_charge":"No","doi":"10.1105/tpc.113.114322","publication_identifier":{"issn":["1040-4651","1532-298X"]},"quality_controlled":"1","status":"public","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87","oa_version":"None","page":"2618-2632","date_updated":"2021-01-12T08:14:24Z","day":"26","publication":"The Plant Cell","citation":{"short":"S. Tan, C. Dai, H.-T. Liu, H.-W. Xue, The Plant Cell 25 (2013) 2618–2632.","chicago":"Tan, Shutang, C. Dai, H.-T. Liu, and H.-W. Xue. “Arabidopsis Casein Kinase1 Proteins CK1.3 and CK1.4 Phosphorylate Cryptochrome2 to Regulate Blue Light Signaling.” <i>The Plant Cell</i>. American Society of Plant Biologists, 2013. <a href=\"https://doi.org/10.1105/tpc.113.114322\">https://doi.org/10.1105/tpc.113.114322</a>.","ista":"Tan S, Dai C, Liu H-T, Xue H-W. 2013. Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling. The Plant Cell. 25(7), 2618–2632.","ama":"Tan S, Dai C, Liu H-T, Xue H-W. Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling. <i>The Plant Cell</i>. 2013;25(7):2618-2632. doi:<a href=\"https://doi.org/10.1105/tpc.113.114322\">10.1105/tpc.113.114322</a>","ieee":"S. Tan, C. Dai, H.-T. Liu, and H.-W. Xue, “Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling,” <i>The Plant Cell</i>, vol. 25, no. 7. American Society of Plant Biologists, pp. 2618–2632, 2013.","mla":"Tan, Shutang, et al. “Arabidopsis Casein Kinase1 Proteins CK1.3 and CK1.4 Phosphorylate Cryptochrome2 to Regulate Blue Light Signaling.” <i>The Plant Cell</i>, vol. 25, no. 7, American Society of Plant Biologists, 2013, pp. 2618–32, doi:<a href=\"https://doi.org/10.1105/tpc.113.114322\">10.1105/tpc.113.114322</a>.","apa":"Tan, S., Dai, C., Liu, H.-T., &#38; Xue, H.-W. (2013). Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling. <i>The Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.113.114322\">https://doi.org/10.1105/tpc.113.114322</a>"},"publisher":"American Society of Plant Biologists","title":"Arabidopsis casein kinase1 proteins CK1.3 and CK1.4 phosphorylate cryptochrome2 to regulate blue light signaling","_id":"7596","author":[{"last_name":"Tan","first_name":"Shutang","id":"2DE75584-F248-11E8-B48F-1D18A9856A87","full_name":"Tan, Shutang","orcid":"0000-0002-0471-8285"},{"first_name":"C.","last_name":"Dai","full_name":"Dai, C."},{"full_name":"Liu, H.-T.","last_name":"Liu","first_name":"H.-T."},{"first_name":"H.-W.","last_name":"Xue","full_name":"Xue, H.-W."}],"external_id":{"pmid":["23897926"]},"publication_status":"published","pmid":1,"volume":25,"abstract":[{"text":"Casein kinase1 (CK1) plays crucial roles in regulating growth and development via phosphorylating various substrates throughout the eukaryote kingdom. Blue light is crucial for normal growth of both plants and animals, and blue light receptor cryptochrome2 (CRY2) undergoes blue light–dependent phosphorylation and degradation in planta. To study the function of plant CK1s, systematic genetic analysis showed that deficiency of two paralogous Arabidopsis thaliana CK1s, CK1.3 and CK1.4, caused shortened hypocotyls, especially under blue light, while overexpression of either CK1.3 or CK1.4 resulted in the insensitive response to blue light and delayed flowering under long-day conditions. CK1.3 or CK1.4 act dependently on CRY2, and overexpression of CK1.3 or CK1.4 significantly suppresses the hypersensitive response to blue light by CRY2 overexpression. Biochemical studies showed that CK1.3 and CK1.4 directly phosphorylate CRY2 at Ser-587 and Thr-603 in vitro and negatively regulate CRY2 stability in planta, which are stimulated by blue light, further confirming the crucial roles of CK1.3 and CK1.4 in blue light responses through phosphorylating CRY2. Interestingly, expression of CK1.3 and CK1.4 is stimulated by blue light and feedback regulated by CRY2-mediated signaling. These results provide direct evidence for CRY2 phosphorylation and informative clues on the mechanisms of CRY2-mediated light responses.","lang":"eng"}],"month":"08","issue":"7","language":[{"iso":"eng"}],"intvolume":"        25","date_published":"2013-08-26T00:00:00Z","year":"2013","article_type":"original","date_created":"2020-03-21T16:06:55Z"},{"date_published":"2003-03-01T00:00:00Z","article_type":"original","year":"2003","publist_id":"3710","date_created":"2018-12-11T12:00:44Z","language":[{"iso":"eng"}],"issue":"3","intvolume":"        15","abstract":[{"text":"Plants have many polarized cell types, but relatively little is known about the mechanisms that establish polarity. The orc mutant was identified originally by defects in root patterning, and positional cloning revealed that the affected gene encodes STEROL METHYLTRANSFERASE1, which is required for the appropriate synthesis and composition of major membrane sterols. smt1orc mutants displayed several conspicuous cell polarity defects. Columella root cap cells revealed perturbed polar positioning of different organelles, and in the smt1orc root epidermis, polar initiation of root hairs was more randomized. Polar auxin transport and expression of the auxin reporter DR5-β-glucuronidase were aberrant in smt1orc. Patterning defects in smt1orc resembled those observed in mutants of the PIN gene family of putative auxin efflux transporters. Consistently, the membrane localization of the PIN1 and PIN3 proteins was disturbed in smt1orc, whereas polar positioning of the influx carrier AUX1 appeared normal. Our results suggest that balanced sterol composition is a major requirement for cell polarity and auxin efflux in Arabidopsis.","lang":"eng"}],"month":"03","volume":15,"external_id":{"pmid":["12615936"]},"publication_status":"published","pmid":1,"author":[{"full_name":"Willemsen, Viola","first_name":"Viola","last_name":"Willemsen"},{"orcid":"0000-0002-8302-7596","full_name":"Friml, Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml"},{"last_name":"Grebe","first_name":"Markus","full_name":"Grebe, Markus"},{"first_name":"Albert","last_name":"Van Den Toorn","full_name":"Van Den Toorn, Albert"},{"full_name":"Palme, Klaus","first_name":"Klaus","last_name":"Palme"},{"full_name":"Scheres, Ben","last_name":"Scheres","first_name":"Ben"}],"_id":"2992","title":"Cell polarity and PIN protein positioning in Arabidopsis require STEROL METHYLTRANSFERASE1 function","publication":"Plant Cell","citation":{"apa":"Willemsen, V., Friml, J., Grebe, M., Van Den Toorn, A., Palme, K., &#38; Scheres, B. (2003). Cell polarity and PIN protein positioning in Arabidopsis require STEROL METHYLTRANSFERASE1 function. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.008433\">https://doi.org/10.1105/tpc.008433</a>","mla":"Willemsen, Viola, et al. “Cell Polarity and PIN Protein Positioning in Arabidopsis Require STEROL METHYLTRANSFERASE1 Function.” <i>Plant Cell</i>, vol. 15, no. 3, American Society of Plant Biologists, 2003, pp. 612–25, doi:<a href=\"https://doi.org/10.1105/tpc.008433\">10.1105/tpc.008433</a>.","ieee":"V. Willemsen, J. Friml, M. Grebe, A. Van Den Toorn, K. Palme, and B. Scheres, “Cell polarity and PIN protein positioning in Arabidopsis require STEROL METHYLTRANSFERASE1 function,” <i>Plant Cell</i>, vol. 15, no. 3. American Society of Plant Biologists, pp. 612–625, 2003.","ama":"Willemsen V, Friml J, Grebe M, Van Den Toorn A, Palme K, Scheres B. Cell polarity and PIN protein positioning in Arabidopsis require STEROL METHYLTRANSFERASE1 function. <i>Plant Cell</i>. 2003;15(3):612-625. doi:<a href=\"https://doi.org/10.1105/tpc.008433\">10.1105/tpc.008433</a>","ista":"Willemsen V, Friml J, Grebe M, Van Den Toorn A, Palme K, Scheres B. 2003. Cell polarity and PIN protein positioning in Arabidopsis require STEROL METHYLTRANSFERASE1 function. Plant Cell. 15(3), 612–625.","chicago":"Willemsen, Viola, Jiří Friml, Markus Grebe, Albert Van Den Toorn, Klaus Palme, and Ben Scheres. “Cell Polarity and PIN Protein Positioning in Arabidopsis Require STEROL METHYLTRANSFERASE1 Function.” <i>Plant Cell</i>. American Society of Plant Biologists, 2003. <a href=\"https://doi.org/10.1105/tpc.008433\">https://doi.org/10.1105/tpc.008433</a>.","short":"V. Willemsen, J. Friml, M. Grebe, A. Van Den Toorn, K. Palme, B. Scheres, Plant Cell 15 (2003) 612–625."},"publisher":"American Society of Plant Biologists","day":"01","date_updated":"2026-05-22T09:04:01Z","OA_type":"closed access","scopus_import":"1","user_id":"ba8df636-2132-11f1-aed0-ed93e2281fdd","oa_version":"None","page":"612 - 625","publication_identifier":{"eissn":["1532-298X"],"issn":["1040-4651"]},"doi":"10.1105/tpc.008433","quality_controlled":"1","status":"public","type":"journal_article","extern":"1","article_processing_charge":"No"},{"quality_controlled":"1","publication_identifier":{"issn":["1040-4651"]},"doi":"10.1105/tpc.001248","status":"public","page":"1017 - 1031","user_id":"ea97e931-d5af-11eb-85d4-e6957dddbf17","scopus_import":"1","oa_version":"None","extern":"1","type":"journal_article","article_processing_charge":"No","citation":{"ama":"Souter M, Topping J, Pullen M, et al. Hydra mutants of Arabidopsis are defective in sterol profiles and auxin and ethylene signaling. <i>Plant Cell</i>. 2002;14(5):1017-1031. doi:<a href=\"https://doi.org/10.1105/tpc.001248\">10.1105/tpc.001248</a>","ieee":"M. Souter <i>et al.</i>, “Hydra mutants of Arabidopsis are defective in sterol profiles and auxin and ethylene signaling,” <i>Plant Cell</i>, vol. 14, no. 5. American Society of Plant Biologists, pp. 1017–1031, 2002.","apa":"Souter, M., Topping, J., Pullen, M., Friml, J., Palme, K., Hackett, R., … Lindsey, K. (2002). Hydra mutants of Arabidopsis are defective in sterol profiles and auxin and ethylene signaling. <i>Plant Cell</i>. American Society of Plant Biologists. <a href=\"https://doi.org/10.1105/tpc.001248\">https://doi.org/10.1105/tpc.001248</a>","mla":"Souter, Martin, et al. “Hydra Mutants of Arabidopsis Are Defective in Sterol Profiles and Auxin and Ethylene Signaling.” <i>Plant Cell</i>, vol. 14, no. 5, American Society of Plant Biologists, 2002, pp. 1017–31, doi:<a href=\"https://doi.org/10.1105/tpc.001248\">10.1105/tpc.001248</a>.","short":"M. Souter, J. Topping, M. Pullen, J. Friml, K. Palme, R. Hackett, D. Grierson, K. Lindsey, Plant Cell 14 (2002) 1017–1031.","chicago":"Souter, Martin, Jennifer Topping, Margaret Pullen, Jiří Friml, Klaus Palme, Rachel Hackett, Don Grierson, and Keith Lindsey. “Hydra Mutants of Arabidopsis Are Defective in Sterol Profiles and Auxin and Ethylene Signaling.” <i>Plant Cell</i>. American Society of Plant Biologists, 2002. <a href=\"https://doi.org/10.1105/tpc.001248\">https://doi.org/10.1105/tpc.001248</a>.","ista":"Souter M, Topping J, Pullen M, Friml J, Palme K, Hackett R, Grierson D, Lindsey K. 2002. Hydra mutants of Arabidopsis are defective in sterol profiles and auxin and ethylene signaling. Plant Cell. 14(5), 1017–1031."},"publication":"Plant Cell","publisher":"American Society of Plant Biologists","main_file_link":[{"url":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC150604/","open_access":"1"}],"title":"Hydra mutants of Arabidopsis are defective in sterol profiles and auxin and ethylene signaling","_id":"2987","date_updated":"2023-07-18T07:34:32Z","day":"01","author":[{"last_name":"Souter","first_name":"Martin","full_name":"Souter, Martin"},{"last_name":"Topping","first_name":"Jennifer","full_name":"Topping, Jennifer"},{"full_name":"Pullen, Margaret","first_name":"Margaret","last_name":"Pullen"},{"full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596","id":"4159519E-F248-11E8-B48F-1D18A9856A87","first_name":"Jirí","last_name":"Friml"},{"last_name":"Palme","first_name":"Klaus","full_name":"Palme, Klaus"},{"last_name":"Hackett","first_name":"Rachel","full_name":"Hackett, Rachel"},{"first_name":"Don","last_name":"Grierson","full_name":"Grierson, Don"},{"first_name":"Keith","last_name":"Lindsey","full_name":"Lindsey, Keith"}],"external_id":{"pmid":["12034894"]},"pmid":1,"publication_status":"published","oa":1,"issue":"5","acknowledgement":"We thank Dr. Ken Feldmann for providing prospective hyd alleles, Dr. Jane Murfett for providing DR5::GUS seed, Dr. D. Van Der Straeten for providing ACS1::GUS seed, Dr. John Schiefelbein for providing GL2::GFP seed, and Dr. Ottoline Leyser for axr1-12 and axr3-1 seed. etr1 and fk seed was obtained from the Nottingham Arabidopsis Stock Centre. This work was supported by a Biotechnology and Biological Science Research Council research studentship to M.S., a Durham University studentship to M.P., and Biotechnology and Biological Science Research Council Grant 12/P02330 to J.T.","language":[{"iso":"eng"}],"intvolume":"        14","article_type":"original","publist_id":"3716","year":"2002","date_published":"2002-05-01T00:00:00Z","date_created":"2018-12-11T12:00:42Z","volume":14,"abstract":[{"text":"The hydra mutants of Arabidopsis are characterized by a pleiotropic phenotype that shows defective embryonic and seedling cell patterning, morphogenesis, and root growth. We demonstrate that the HYDRA1 gene encodes a Δ8-Δ7 sterol isomerase, whereas HYDRA2 encodes a sterol C14 reductase, previously identified as the FACKEL gene product. Seedlings mutant for each gene are similarly defective in the concentrations of the three major Arabidopsis sterols. Promoter::reporter gene analysis showed misexpression of the auxin-regulated DR5 and ACS1 promoters and of the epidermal cell file-specific GL2 promoter in the mutants. The mutants exhibit enhanced responses to auxin. The phenotypes can be rescued partially by inhibition of auxin and ethylene signaling but not by exogenous sterols or brassinosteroids. We propose a model in which correct sterol profiles are required for regulated auxin and ethylene signaling through effects on membrane function.","lang":"eng"}],"month":"05"}]