---
_id: '1017'
abstract:
- lang: eng
  text: The development of the vertebrate central nervous system is reliant on a complex
    cascade of biological processes that include mitotic division, relocation of migrating
    neurons, and the extension of dendritic and axonal processes. Each of these cellular
    events requires the diverse functional repertoire of the microtubule cytoskeleton
    for the generation of forces, assembly of macromolecular complexes and transport
    of molecules and organelles. The tubulins are a multi-gene family that encode
    for the constituents of microtubules, and have been implicated in a spectrum of
    neurological disorders. Evidence is building that different tubulins tune the
    functional properties of the microtubule cytoskeleton dependent on the cell type,
    developmental profile and subcellular localisation. Here we review of the origins
    of the functional specification of the tubulin gene family in the developing brain
    at a transcriptional, translational, and post-transcriptional level. We remind
    the reader that tubulins are not just loading controls for your average Western
    blot.
article_processing_charge: No
author:
- first_name: Martin
  full_name: Breuss, Martin
  last_name: Breuss
- first_name: Ines
  full_name: Leca, Ines
  last_name: Leca
- first_name: Thomas
  full_name: Gstrein, Thomas
  last_name: Gstrein
- first_name: Andi H
  full_name: Hansen, Andi H
  id: 38853E16-F248-11E8-B48F-1D18A9856A87
  last_name: Hansen
- first_name: David
  full_name: Keays, David
  last_name: Keays
citation:
  ama: 'Breuss M, Leca I, Gstrein T, Hansen AH, Keays D. Tubulins and brain development:
    The origins of functional specification. <i>Molecular and Cellular Neuroscience</i>.
    2017;84:58-67. doi:<a href="https://doi.org/10.1016/j.mcn.2017.03.002">10.1016/j.mcn.2017.03.002</a>'
  apa: 'Breuss, M., Leca, I., Gstrein, T., Hansen, A. H., &#38; Keays, D. (2017).
    Tubulins and brain development: The origins of functional specification. <i>Molecular
    and Cellular Neuroscience</i>. Academic Press. <a href="https://doi.org/10.1016/j.mcn.2017.03.002">https://doi.org/10.1016/j.mcn.2017.03.002</a>'
  chicago: 'Breuss, Martin, Ines Leca, Thomas Gstrein, Andi H Hansen, and David Keays.
    “Tubulins and Brain Development: The Origins of Functional Specification.” <i>Molecular
    and Cellular Neuroscience</i>. Academic Press, 2017. <a href="https://doi.org/10.1016/j.mcn.2017.03.002">https://doi.org/10.1016/j.mcn.2017.03.002</a>.'
  ieee: 'M. Breuss, I. Leca, T. Gstrein, A. H. Hansen, and D. Keays, “Tubulins and
    brain development: The origins of functional specification,” <i>Molecular and
    Cellular Neuroscience</i>, vol. 84. Academic Press, pp. 58–67, 2017.'
  ista: 'Breuss M, Leca I, Gstrein T, Hansen AH, Keays D. 2017. Tubulins and brain
    development: The origins of functional specification. Molecular and Cellular Neuroscience.
    84, 58–67.'
  mla: 'Breuss, Martin, et al. “Tubulins and Brain Development: The Origins of Functional
    Specification.” <i>Molecular and Cellular Neuroscience</i>, vol. 84, Academic
    Press, 2017, pp. 58–67, doi:<a href="https://doi.org/10.1016/j.mcn.2017.03.002">10.1016/j.mcn.2017.03.002</a>.'
  short: M. Breuss, I. Leca, T. Gstrein, A.H. Hansen, D. Keays, Molecular and Cellular
    Neuroscience 84 (2017) 58–67.
date_created: 2018-12-11T11:49:42Z
date_published: 2017-10-01T00:00:00Z
date_updated: 2025-07-10T11:49:44Z
day: '01'
ddc:
- '571'
department:
- _id: SiHi
doi: 10.1016/j.mcn.2017.03.002
external_id:
  isi:
  - '000415140700007'
file:
- access_level: open_access
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:09:19Z
  date_updated: 2018-12-12T10:09:19Z
  file_id: '4742'
  file_name: IST-2017-806-v1+2_1-s2.0-S1044743116302500-main_1_.pdf
  file_size: 1436377
  relation: main_file
file_date_updated: 2018-12-12T10:09:19Z
has_accepted_license: '1'
intvolume: '        84'
isi: 1
language:
- iso: eng
month: '10'
oa: 1
oa_version: Published Version
page: 58 - 67
publication: Molecular and Cellular Neuroscience
publication_identifier:
  issn:
  - 1044-7431
publication_status: published
publisher: Academic Press
publist_id: '6377'
pubrep_id: '806'
quality_controlled: '1'
scopus_import: '1'
status: public
title: 'Tubulins and brain development: The origins of functional specification'
tmp:
  image: /images/cc_by_nc_nd.png
  legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
    (CC BY-NC-ND 4.0)
  short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 84
year: '2017'
...
---
_id: '2607'
abstract:
- lang: eng
  text: Alternative splicing in the mGluR5 gene generates two different receptor isoforms,
    of which expression is developmentally regulated. However, little is known about
    the functional significance of mGluR5 splice variants. We have examined the functional
    coupling, subcellular targeting, and effect on neuronal differentiation of epitope-tagged
    mGluR5 isoforms by expression in neuroblastoma NG108-15 cells. We found that both
    mGluR5 splice variants give rise to comparable [Ca2+]i transients and have similar
    pharmacological profile. Tagged receptors were shown by immunofluorescence to
    be inserted in the plasma membrane. In undifferentiated cells the subcellular
    localization of the two mGluR5 isoforms was partially segregated, whereas in differentiated
    cells the labeling largely redistributed to the newly formed neurites. Interestingly,
    we demonstrate that mGluR5 splice variants dramatically influence the formation
    and maturation of neurites; mGluR5a hinders the acquisition of mature neuronal
    traits and mGluR5b fosters the elaboration and extension of neurites. These effects
    are partly inhibited by MPEP.
acknowledgement: "The authors thank: Dr. J. M. Rimland and M. T. Scupoli for their
  technical help with X. oocytes recordings and FAC sorting, respectively; Dr. Y.
  Dalezios for helping with the statistical analyses; and Dr. G. Varani for helping
  with the analyses of mRNA and genomic sequences. We are also grateful to Professor
  F. Benfenati, Dr. F. Conquet, Dr. Rafael Lujan, Dr. J. McIlhinney, Professor P.
  Somogyi, Dr. J. H. Xuereb, and Dr. M. Zoli for careful reading of the manuscript\r\nand
  helpful suggestions. R.S. is supported by the Laboratory of Cerebral Structure,
  National Institute for Physiological Sciences, Myodaiji, Okazaki 444-8585, CREST
  Japan Science and Technology Corporation, Japan."
article_processing_charge: No
article_type: original
author:
- first_name: Silvia
  full_name: Mion, Silvia
  last_name: Mion
- first_name: Corrado
  full_name: Corti, Corrado
  last_name: Corti
- first_name: Akio
  full_name: Neki, Akio
  last_name: Neki
- first_name: Ryuichi
  full_name: Shigemoto, Ryuichi
  id: 499F3ABC-F248-11E8-B48F-1D18A9856A87
  last_name: Shigemoto
  orcid: 0000-0001-8761-9444
- first_name: Mauro
  full_name: Corsi, Mauro
  last_name: Corsi
- first_name: Guido
  full_name: Fumagalli, Guido
  last_name: Fumagalli
- first_name: Francesco
  full_name: Ferraguti, Francesco
  last_name: Ferraguti
citation:
  ama: Mion S, Corti C, Neki A, et al. Bidirectional regulation of neurite elaboration
    by alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms.
    <i>Molecular and Cellular Neuroscience</i>. 2001;17(6):957-972. doi:<a href="https://doi.org/10.1006/mcne.2001.0993">10.1006/mcne.2001.0993</a>
  apa: Mion, S., Corti, C., Neki, A., Shigemoto, R., Corsi, M., Fumagalli, G., &#38;
    Ferraguti, F. (2001). Bidirectional regulation of neurite elaboration by alternatively
    spliced metabotropic glutamate receptor 5 (mGluR5) isoforms. <i>Molecular and
    Cellular Neuroscience</i>. Academic Press. <a href="https://doi.org/10.1006/mcne.2001.0993">https://doi.org/10.1006/mcne.2001.0993</a>
  chicago: Mion, Silvia, Corrado Corti, Akio Neki, Ryuichi Shigemoto, Mauro Corsi,
    Guido Fumagalli, and Francesco Ferraguti. “Bidirectional Regulation of Neurite
    Elaboration by Alternatively Spliced Metabotropic Glutamate Receptor 5 (MGluR5)
    Isoforms.” <i>Molecular and Cellular Neuroscience</i>. Academic Press, 2001. <a
    href="https://doi.org/10.1006/mcne.2001.0993">https://doi.org/10.1006/mcne.2001.0993</a>.
  ieee: S. Mion <i>et al.</i>, “Bidirectional regulation of neurite elaboration by
    alternatively spliced metabotropic glutamate receptor 5 (mGluR5) isoforms,” <i>Molecular
    and Cellular Neuroscience</i>, vol. 17, no. 6. Academic Press, pp. 957–972, 2001.
  ista: Mion S, Corti C, Neki A, Shigemoto R, Corsi M, Fumagalli G, Ferraguti F. 2001.
    Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic
    glutamate receptor 5 (mGluR5) isoforms. Molecular and Cellular Neuroscience. 17(6),
    957–972.
  mla: Mion, Silvia, et al. “Bidirectional Regulation of Neurite Elaboration by Alternatively
    Spliced Metabotropic Glutamate Receptor 5 (MGluR5) Isoforms.” <i>Molecular and
    Cellular Neuroscience</i>, vol. 17, no. 6, Academic Press, 2001, pp. 957–72, doi:<a
    href="https://doi.org/10.1006/mcne.2001.0993">10.1006/mcne.2001.0993</a>.
  short: S. Mion, C. Corti, A. Neki, R. Shigemoto, M. Corsi, G. Fumagalli, F. Ferraguti,
    Molecular and Cellular Neuroscience 17 (2001) 957–972.
date_created: 2018-12-11T11:58:38Z
date_published: 2001-06-01T00:00:00Z
date_updated: 2023-05-24T09:34:13Z
day: '01'
doi: 10.1006/mcne.2001.0993
extern: '1'
external_id:
  pmid:
  - '11414786'
intvolume: '        17'
issue: '6'
language:
- iso: eng
month: '06'
oa_version: None
page: 957 - 972
pmid: 1
publication: Molecular and Cellular Neuroscience
publication_identifier:
  issn:
  - 1044-7431
publication_status: published
publisher: Academic Press
publist_id: '4291'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Bidirectional regulation of neurite elaboration by alternatively spliced metabotropic
  glutamate receptor 5 (mGluR5) isoforms
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 17
year: '2001'
...