---
OA_type: closed access
_id: '23126'
abstract:
- lang: eng
text: Plant grafting is a powerful tool in research and agriculture, enabling the
study of long-distance signaling and improving crop performance. Hypocotyl grafting
in Arabidopsis thaliana has become a key method for investigating inter-tissue
communication, root-to-shoot transport, and vascular regeneration. Here, we describe
a high-throughput hypocotyl grafting protocol that consistently achieves success
rates above 90% while minimizing complications, such as adventitious root formation,
through an optimized hypocotyl cutting position. Our streamlined approach eliminates
the need for silicone tubes and fine forceps, requiring only a standard scalpel
for the cutting and joining procedure. With proper training, an experienced user
can graft up to 1000 plants per day, facilitating large-scale experiments. Additionally,
we present vascular connectivity assays using fluorescent dyes and the adjuvant
Adigor to enable phloem and xylem transport analysis across the graft junction
without the need for wounding. By simplifying the grafting process while maintaining
high efficiency, our protocol makes Arabidopsis grafting more accessible and scalable,
facilitating research on long-distance molecular transport, vascular development,
and plant biotechnology applications.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Kai
full_name: Bartusch, Kai
id: 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
last_name: Bartusch
orcid: 0000-0003-2708-5545
- first_name: Elisabeth
full_name: Truernit, Elisabeth
last_name: Truernit
citation:
ama: 'Bartusch K, Truernit E. High-Throughput Hypocotyl Grafting and Vascular Reconnection
Assessment in Arabidopsis Seedlings. In: Liesche J, Sevanto S, eds. Phloem.
2nd ed. MIMB. New York, NY: Springer; 2026:431-448. doi:10.1007/978-1-0716-5488-0_33'
apa: 'Bartusch, K., & Truernit, E. (2026). High-Throughput Hypocotyl Grafting
and Vascular Reconnection Assessment in Arabidopsis Seedlings. In J. Liesche &
S. Sevanto (Eds.), Phloem (2nd ed., pp. 431–448). New York, NY: Springer.
https://doi.org/10.1007/978-1-0716-5488-0_33'
chicago: 'Bartusch, Kai, and Elisabeth Truernit. “High-Throughput Hypocotyl Grafting
and Vascular Reconnection Assessment in Arabidopsis Seedlings.” In Phloem,
edited by Johannes Liesche and Sanna Sevanto, 2nd ed., 431–48. MIMB. New York,
NY: Springer, 2026. https://doi.org/10.1007/978-1-0716-5488-0_33.'
ieee: 'K. Bartusch and E. Truernit, “High-Throughput Hypocotyl Grafting and Vascular
Reconnection Assessment in Arabidopsis Seedlings,” in Phloem, 2nd ed.,
J. Liesche and S. Sevanto, Eds. New York, NY: Springer, 2026, pp. 431–448.'
ista: 'Bartusch K, Truernit E. 2026.High-Throughput Hypocotyl Grafting and Vascular
Reconnection Assessment in Arabidopsis Seedlings. In: Phloem. Methods in Molecular
Biology, , 431–448.'
mla: Bartusch, Kai, and Elisabeth Truernit. “High-Throughput Hypocotyl Grafting
and Vascular Reconnection Assessment in Arabidopsis Seedlings.” Phloem,
edited by Johannes Liesche and Sanna Sevanto, 2nd ed., Springer, 2026, pp. 431–48,
doi:10.1007/978-1-0716-5488-0_33.
short: K. Bartusch, E. Truernit, in:, J. Liesche, S. Sevanto (Eds.), Phloem, 2nd
ed., Springer, New York, NY, 2026, pp. 431–448.
date_created: 2026-10-13T11:14:23Z
date_published: 2026-09-03T00:00:00Z
date_updated: 2026-10-14T08:58:03Z
day: '03'
doi: 10.1007/978-1-0716-5488-0_33
edition: '2'
editor:
- first_name: Johannes
full_name: Liesche, Johannes
last_name: Liesche
- first_name: Sanna
full_name: Sevanto, Sanna
last_name: Sevanto
extern: '1'
external_id:
pmid:
- '42681141'
fulldoi: https://doi.org/10.1007/978-1-0716-5488-0_33
language:
- iso: eng
month: '09'
oa_version: None
page: 431-448
place: New York, NY
pmid: 1
publication: Phloem
publication_identifier:
eisbn:
- '9781071654880'
eissn:
- 1940-6029
isbn:
- '9781071654873'
issn:
- 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in
Arabidopsis Seedlings
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2026'
...
---
OA_type: closed access
_id: '18765'
abstract:
- lang: eng
text: Mosaic Analysis with Double Markers (MADM) represents a mouse genetic approach
coupling differential fluorescent labeling to genetic manipulations in dividing
cells and their lineages. MADM uniquely enables the generation and visualization
of individual control or homozygous mutant cells in a heterozygous genetic environment.
Among its diverse applications, MADM has been used to dissect cell-autonomous
gene functions important for cortical development and neural development in general.
The high cellular resolution offered by MADM also permits the analysis of transcriptomic
changes of individual cells upon genetic manipulations. In this chapter, we describe
an experimental protocol combining the generation and isolation of MADM-labeled
cells with downstream single-cell RNA-sequencing technologies to probe cell-type
specific phenotypes due to genetic mutations at single-cell resolution.
acknowledged_ssus:
- _id: Bio
acknowledgement: 'We thank all Hippenmeyer lab members for support and discussions.
Experimental steps described were optimized with support provided by the Imaging
& Optics Facility (IOF) and Preclinical Facility (PCF) at ISTA, Vienna BioCenter
Core Facilities (VBCF), and Christoph Bock lab at Center for Molecular Medicine
(CeMM). G.C. received funding from European Commission (IST plus postdoctoral fellowship).
This work was supported by ISTA institutional funds: The Austrian Science Fund Special
Research Programmes (FWF SFB F78 Neuro Stem Modulation) to S.H.'
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Florian
full_name: Pauler, Florian
id: 48EA0138-F248-11E8-B48F-1D18A9856A87
last_name: Pauler
orcid: 0000-0002-7462-0048
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: 'Cheung GT, Pauler F, Hippenmeyer S. Probing Cell-Type Specificity of Mutant
Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM).
In: Garcia-Marques J, Lee T, eds. Lineage Tracing. Vol 2886. MIMB. New
York, NY: Springer Nature; 2025:139-151. doi:10.1007/978-1-0716-4310-5_7'
apa: 'Cheung, G. T., Pauler, F., & Hippenmeyer, S. (2025). Probing Cell-Type
Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis
with Double Markers (MADM). In J. Garcia-Marques & T. Lee (Eds.), Lineage
Tracing (Vol. 2886, pp. 139–151). New York, NY: Springer Nature. https://doi.org/10.1007/978-1-0716-4310-5_7'
chicago: 'Cheung, Giselle T, Florian Pauler, and Simon Hippenmeyer. “Probing Cell-Type
Specificity of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis
with Double Markers (MADM).” In Lineage Tracing, edited by Jorge Garcia-Marques
and Tzumin Lee, 2886:139–51. MIMB. New York, NY: Springer Nature, 2025. https://doi.org/10.1007/978-1-0716-4310-5_7.'
ieee: 'G. T. Cheung, F. Pauler, and S. Hippenmeyer, “Probing Cell-Type Specificity
of Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double
Markers (MADM),” in Lineage Tracing, vol. 2886, J. Garcia-Marques and T.
Lee, Eds. New York, NY: Springer Nature, 2025, pp. 139–151.'
ista: 'Cheung GT, Pauler F, Hippenmeyer S. 2025.Probing Cell-Type Specificity of
Mutant Phenotype at Transcriptomic Level Using Mosaic Analysis with Double Markers
(MADM). In: Lineage Tracing. Methods in Molecular Biology, vol. 2886, 139–151.'
mla: Cheung, Giselle T., et al. “Probing Cell-Type Specificity of Mutant Phenotype
at Transcriptomic Level Using Mosaic Analysis with Double Markers (MADM).” Lineage
Tracing, edited by Jorge Garcia-Marques and Tzumin Lee, vol. 2886, Springer
Nature, 2025, pp. 139–51, doi:10.1007/978-1-0716-4310-5_7.
short: G.T. Cheung, F. Pauler, S. Hippenmeyer, in:, J. Garcia-Marques, T. Lee (Eds.),
Lineage Tracing, Springer Nature, New York, NY, 2025, pp. 139–151.
corr_author: '1'
date_created: 2025-01-07T08:36:47Z
date_published: 2025-01-03T00:00:00Z
date_updated: 2025-04-14T07:43:46Z
day: '03'
department:
- _id: SiHi
doi: 10.1007/978-1-0716-4310-5_7
ec_funded: 1
editor:
- first_name: Jorge
full_name: Garcia-Marques, Jorge
last_name: Garcia-Marques
- first_name: Tzumin
full_name: Lee, Tzumin
last_name: Lee
external_id:
pmid:
- '39745639'
fulldoi: https://doi.org/10.1007/978-1-0716-4310-5_7
intvolume: ' 2886'
language:
- iso: eng
month: '01'
oa_version: None
page: 139-151
place: New York, NY
pmid: 1
project:
- _id: 260C2330-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '754411'
name: ISTplus - Postdoctoral Fellowships
publication: Lineage Tracing
publication_identifier:
eisbn:
- '9781071643105'
eissn:
- 1940-6029
isbn:
- '9781071643099'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Probing Cell-Type Specificity of Mutant Phenotype at Transcriptomic Level Using
Mosaic Analysis with Double Markers (MADM)
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2886
year: '2025'
...
---
_id: '17425'
abstract:
- lang: eng
text: Mosaic Analysis with Double Markers (MADM) is a powerful genetic method typically
used for lineage tracing and to disentangle cell autonomous and tissue-wide roles
of candidate genes with single cell resolution. Given the relatively sparse labeling,
depending on which of the 19 MADM chromosomes one chooses, the MADM approach represents
the perfect opportunity for cell morphology analysis. Various MADM studies include
reports of morphological anomalies and phenotypes in the central nervous system
(CNS). MADM for any candidate gene can easily incorporate morphological analysis
within the experimental workflow. Here, we describe the methods of morphological
cell analysis which we developed in the course of diverse recent MADM studies.
This chapter will specifically focus on methods to quantify aspects of the morphology
of neurons and astrocytes within the CNS, but these methods can broadly be applied
to any MADM-labeled cells throughout the entire organism. We will cover two analyses—soma
volume and dendrite characterization—of physical characteristics of pyramidal
neurons in the somatosensory cortex, and two analyses—volume and Sholl analysis—of
astrocyte morphology.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank all Hippenmeyer lab members for support and discussions.
This work was supported by the Scientific Service Units (SSU) at ISTA through resources
provided by the Imaging & Optics Facility (IOF). O.A.M was a recipient of a DOC
Fellowship (26253) of the Austrian Academy of Sciences. This work was supported
by ISTA institutional funds, and The Austrian Science Fund Special Research Programmes
(FWF SFB F78 Neuro Stem Modulation) to S.H.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Osvaldo
full_name: Miranda, Osvaldo
id: 862A3C56-A8BF-11E9-B4FA-D9E3E5697425
last_name: Miranda
orcid: 0000-0001-6618-6889
- first_name: Giselle T
full_name: Cheung, Giselle T
id: 471195F6-F248-11E8-B48F-1D18A9856A87
last_name: Cheung
orcid: 0000-0001-8457-2572
- first_name: Simon
full_name: Hippenmeyer, Simon
id: 37B36620-F248-11E8-B48F-1D18A9856A87
last_name: Hippenmeyer
orcid: 0000-0003-2279-1061
citation:
ama: 'Miranda O, Cheung GT, Hippenmeyer S. Morphological Analysis of Neurons and
Glia Using Mosaic Analysis with Double Markers. In: Toyooka K, ed. Neuronal
Morphogenesis. Vol 2831. 1st ed. MIMB. New York, NY: Springer Nature; 2024:283-299.
doi:10.1007/978-1-0716-3969-6_19'
apa: 'Miranda, O., Cheung, G. T., & Hippenmeyer, S. (2024). Morphological Analysis
of Neurons and Glia Using Mosaic Analysis with Double Markers. In K. Toyooka (Ed.),
Neuronal Morphogenesis (1st ed., Vol. 2831, pp. 283–299). New York, NY:
Springer Nature. https://doi.org/10.1007/978-1-0716-3969-6_19'
chicago: 'Miranda, Osvaldo, Giselle T Cheung, and Simon Hippenmeyer. “Morphological
Analysis of Neurons and Glia Using Mosaic Analysis with Double Markers.” In Neuronal
Morphogenesis, edited by Kazuhito Toyooka, 1st ed., 2831:283–99. MIMB. New
York, NY: Springer Nature, 2024. https://doi.org/10.1007/978-1-0716-3969-6_19.'
ieee: 'O. Miranda, G. T. Cheung, and S. Hippenmeyer, “Morphological Analysis of
Neurons and Glia Using Mosaic Analysis with Double Markers,” in Neuronal Morphogenesis,
1st ed., vol. 2831, K. Toyooka, Ed. New York, NY: Springer Nature, 2024, pp. 283–299.'
ista: 'Miranda O, Cheung GT, Hippenmeyer S. 2024.Morphological Analysis of Neurons
and Glia Using Mosaic Analysis with Double Markers. In: Neuronal Morphogenesis.
Methods in Molecular Biology, vol. 2831, 283–299.'
mla: Miranda, Osvaldo, et al. “Morphological Analysis of Neurons and Glia Using
Mosaic Analysis with Double Markers.” Neuronal Morphogenesis, edited by
Kazuhito Toyooka, 1st ed., vol. 2831, Springer Nature, 2024, pp. 283–99, doi:10.1007/978-1-0716-3969-6_19.
short: O. Miranda, G.T. Cheung, S. Hippenmeyer, in:, K. Toyooka (Ed.), Neuronal
Morphogenesis, 1st ed., Springer Nature, New York, NY, 2024, pp. 283–299.
corr_author: '1'
das_tickbox: '0'
date_created: 2024-08-13T12:16:41Z
date_published: 2024-08-13T00:00:00Z
date_updated: 2026-11-11T23:31:18Z
day: '13'
department:
- _id: GradSch
- _id: SiHi
doi: 10.1007/978-1-0716-3969-6_19
edition: '1'
editor:
- first_name: Kazuhito
full_name: Toyooka, Kazuhito
last_name: Toyooka
external_id:
pmid:
- '39134857'
fulldoi: https://doi.org/10.1007/978-1-0716-3969-6_19
intvolume: ' 2831'
language:
- iso: eng
month: '08'
oa_version: None
page: 283-299
place: New York, NY
pmid: 1
project:
- _id: 34c9fbcb-11ca-11ed-8bc3-98fa5658610d
grant_number: '26253'
name: Molecular Mechanisms Regulating Cortical Neural Stem Cell Lineage Progression
and Astrocyte Development
- _id: 059F6AB4-7A3F-11EA-A408-12923DDC885E
grant_number: F7805
name: Stem Cell Modulation in Neural Development and Regeneration/ P05-Molecular
Mechanisms of Neural Stem Cell Lineage Progression
publication: Neuronal Morphogenesis
publication_identifier:
eisbn:
- '9781071639696'
eissn:
- 1940-6029
isbn:
- '9781071639689'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
related_material:
record:
- id: '20212'
relation: dissertation_contains
status: public
researchdata_availability: no
scopus_import: '1'
series_title: MIMB
status: public
supplementarymaterial: yes
title: Morphological Analysis of Neurons and Glia Using Mosaic Analysis with Double
Markers
type: book_chapter
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 2831
year: '2024'
...
---
OA_place: repository
OA_type: green
_id: '12720'
abstract:
- lang: eng
text: Here we describe the in vivo DNA assembly approach, where molecular cloning
procedures are performed using an E. coli recA-independent recombination pathway,
which assembles linear fragments of DNA with short homologous termini. This pathway
is present in all standard laboratory E. coli strains and, by bypassing the need
for in vitro DNA assembly, allows simplified molecular cloning to be performed
without the plasmid instability issues associated with specialized recombination-cloning
bacterial strains. The methodology requires specific primer design and can perform
all standard plasmid modifications (insertions, deletions, mutagenesis, and sub-cloning)
in a rapid, simple, and cost-efficient manner, as it does not require commercial
kits or specialized bacterial strains. Additionally, this approach can be used
to perform complex procedures such as multiple modifications to a plasmid, as
up to 6 linear fragments can be assembled in vivo by this recombination pathway.
Procedures generally require less than 3 h, involving PCR amplification, DpnI
digestion of template DNA, and transformation, upon which circular plasmids are
assembled. In this chapter we describe the requirements, procedure, and potential
pitfalls when using this technique, as well as protocol variations to overcome
the most common issues.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Sandra
full_name: Arroyo-Urea, Sandra
last_name: Arroyo-Urea
- first_name: Jake
full_name: Watson, Jake
id: 63836096-4690-11EA-BD4E-32803DDC885E
last_name: Watson
orcid: 0000-0002-8698-3823
- first_name: Javier
full_name: García-Nafría, Javier
last_name: García-Nafría
citation:
ama: 'Arroyo-Urea S, Watson J, García-Nafría J. Molecular Cloning Using In Vivo
DNA Assembly. In: Scarlett G, ed. DNA Manipulation and Analysis. Vol 2633.
MIMB. New York, NY, United States: Springer Nature; 2023:33-44. doi:10.1007/978-1-0716-3004-4_3'
apa: 'Arroyo-Urea, S., Watson, J., & García-Nafría, J. (2023). Molecular Cloning
Using In Vivo DNA Assembly. In G. Scarlett (Ed.), DNA Manipulation and Analysis
(Vol. 2633, pp. 33–44). New York, NY, United States: Springer Nature. https://doi.org/10.1007/978-1-0716-3004-4_3'
chicago: 'Arroyo-Urea, Sandra, Jake Watson, and Javier García-Nafría. “Molecular
Cloning Using In Vivo DNA Assembly.” In DNA Manipulation and Analysis,
edited by Garry Scarlett, 2633:33–44. MIMB. New York, NY, United States: Springer
Nature, 2023. https://doi.org/10.1007/978-1-0716-3004-4_3.'
ieee: 'S. Arroyo-Urea, J. Watson, and J. García-Nafría, “Molecular Cloning Using
In Vivo DNA Assembly,” in DNA Manipulation and Analysis, vol. 2633, G.
Scarlett, Ed. New York, NY, United States: Springer Nature, 2023, pp. 33–44.'
ista: 'Arroyo-Urea S, Watson J, García-Nafría J. 2023.Molecular Cloning Using In
Vivo DNA Assembly. In: DNA Manipulation and Analysis. Methods in Molecular Biology,
vol. 2633, 33–44.'
mla: Arroyo-Urea, Sandra, et al. “Molecular Cloning Using In Vivo DNA Assembly.”
DNA Manipulation and Analysis, edited by Garry Scarlett, vol. 2633, Springer
Nature, 2023, pp. 33–44, doi:10.1007/978-1-0716-3004-4_3.
short: S. Arroyo-Urea, J. Watson, J. García-Nafría, in:, G. Scarlett (Ed.), DNA
Manipulation and Analysis, Springer Nature, New York, NY, United States, 2023,
pp. 33–44.
date_created: 2023-03-12T23:01:02Z
date_published: 2023-03-01T00:00:00Z
date_updated: 2025-06-25T05:56:45Z
day: '01'
department:
- _id: PeJo
doi: 10.1007/978-1-0716-3004-4_3
editor:
- first_name: Garry
full_name: Scarlett, Garry
last_name: Scarlett
external_id:
pmid:
- '36853454'
fulldoi: https://doi.org/10.1007/978-1-0716-3004-4_3
intvolume: ' 2633'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://zaguan.unizar.es/record/125930/files/texto_completo.pdf
month: '03'
oa: 1
oa_version: Submitted Version
page: 33-44
place: New York, NY, United States
pmid: 1
publication: DNA Manipulation and Analysis
publication_identifier:
eisbn:
- 978-1-0716-3004-4
eissn:
- 1940-6029
isbn:
- 978-1-0716-3003-7
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Molecular Cloning Using In Vivo DNA Assembly
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2633
year: '2023'
...
---
_id: '13052'
abstract:
- lang: eng
text: Imaging of the immunological synapse (IS) between dendritic cells (DCs) and
T cells in suspension is hampered by suboptimal alignment of cell-cell contacts
along the vertical imaging plane. This requires optical sectioning that often
results in unsatisfactory resolution in time and space. Here, we present a workflow
where DCs and T cells are confined between a layer of glass and polydimethylsiloxane
(PDMS) that orients the cells along one, horizontal imaging plane, allowing for
fast en-face-imaging of the DC-T cell IS.
acknowledged_ssus:
- _id: Bio
- _id: NanoFab
- _id: M-Shop
acknowledgement: 'A.L. was funded by an Erwin Schrödinger postdoctoral fellowship
of the Austrian Science Fund (FWF, project number: J4542-B) and is an EMBO non-stipendiary
postdoctoral fellow. This work was supported by a European Research Council grant
ERC-CoG-72437 to M.S. We thank the Imaging & Optics facility, the Nanofabrication
facility, and the Miba Machine Shop of ISTA for their excellent support.'
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Alexander F
full_name: Leithner, Alexander F
id: 3B1B77E4-F248-11E8-B48F-1D18A9856A87
last_name: Leithner
orcid: 0000-0002-1073-744X
- first_name: Jack
full_name: Merrin, Jack
id: 4515C308-F248-11E8-B48F-1D18A9856A87
last_name: Merrin
orcid: 0000-0001-5145-4609
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: 'Leithner AF, Merrin J, Sixt MK. En-Face Imaging of T Cell-Dendritic Cell Immunological
Synapses. In: Baldari C, Dustin M, eds. The Immune Synapse. Vol 2654. MIMB.
New York, NY: Springer Nature; 2023:137-147. doi:10.1007/978-1-0716-3135-5_9'
apa: 'Leithner, A. F., Merrin, J., & Sixt, M. K. (2023). En-Face Imaging of
T Cell-Dendritic Cell Immunological Synapses. In C. Baldari & M. Dustin (Eds.),
The Immune Synapse (Vol. 2654, pp. 137–147). New York, NY: Springer Nature.
https://doi.org/10.1007/978-1-0716-3135-5_9'
chicago: 'Leithner, Alexander F, Jack Merrin, and Michael K Sixt. “En-Face Imaging
of T Cell-Dendritic Cell Immunological Synapses.” In The Immune Synapse,
edited by Cosima Baldari and Michael Dustin, 2654:137–47. MIMB. New York, NY:
Springer Nature, 2023. https://doi.org/10.1007/978-1-0716-3135-5_9.'
ieee: 'A. F. Leithner, J. Merrin, and M. K. Sixt, “En-Face Imaging of T Cell-Dendritic
Cell Immunological Synapses,” in The Immune Synapse, vol. 2654, C. Baldari
and M. Dustin, Eds. New York, NY: Springer Nature, 2023, pp. 137–147.'
ista: 'Leithner AF, Merrin J, Sixt MK. 2023.En-Face Imaging of T Cell-Dendritic
Cell Immunological Synapses. In: The Immune Synapse. Methods in Molecular Biology,
vol. 2654, 137–147.'
mla: Leithner, Alexander F., et al. “En-Face Imaging of T Cell-Dendritic Cell Immunological
Synapses.” The Immune Synapse, edited by Cosima Baldari and Michael Dustin,
vol. 2654, Springer Nature, 2023, pp. 137–47, doi:10.1007/978-1-0716-3135-5_9.
short: A.F. Leithner, J. Merrin, M.K. Sixt, in:, C. Baldari, M. Dustin (Eds.), The
Immune Synapse, Springer Nature, New York, NY, 2023, pp. 137–147.
date_created: 2023-05-22T08:41:48Z
date_published: 2023-04-28T00:00:00Z
date_updated: 2025-04-14T07:42:07Z
day: '28'
department:
- _id: MiSi
- _id: NanoFab
doi: 10.1007/978-1-0716-3135-5_9
ec_funded: 1
editor:
- first_name: Cosima
full_name: Baldari, Cosima
last_name: Baldari
- first_name: Michael
full_name: Dustin, Michael
last_name: Dustin
external_id:
pmid:
- '37106180'
fulldoi: https://doi.org/10.1007/978-1-0716-3135-5_9
intvolume: ' 2654'
language:
- iso: eng
month: '04'
oa_version: None
page: 137-147
place: New York, NY
pmid: 1
project:
- _id: 25FE9508-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '724373'
name: Cellular Navigation Along Spatial Gradients
publication: The Immune Synapse
publication_identifier:
eisbn:
- '9781071631355'
eissn:
- 1940-6029
isbn:
- '9781071631348'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: En-Face Imaging of T Cell-Dendritic Cell Immunological Synapses
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2654
year: '2023'
...
---
OA_type: closed access
_id: '23130'
abstract:
- lang: eng
text: Fluorescent dyes are often used to observe transport mechanisms in plant vascular
tissues. However, it has been technically challenging to apply fluorescent dyes
on roots to monitor xylem transport in vivo. Here, we present a fast, noninvasive,
and high-throughput protocol to monitor xylem transport in seedlings. Using the
fluorescent dyes 5(6)-carboxyfluorescein diacetate (CFDA) and Rhodamine WT, we
were able to observe xylem transport on a cellular level in Arabidopsis thaliana
roots. We describe how to apply these dyes on primary roots of young seedlings,
how to monitor root-to-shoot xylem transport, and how to measure xylem transport
velocity in roots. Moreover, we show that our protocol can also be applied to
lateral roots and grafted seedlings to assess xylem (re)connection. Altogether,
these techniques are useful for investigating xylem functionality in diverse experimental
setups.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Kai
full_name: Bartusch, Kai
id: 0c51b9f5-90c8-11f1-b4ef-9ad5d3d9a34a
last_name: Bartusch
orcid: 0000-0003-2708-5545
- first_name: Noel
full_name: Blanco-Touriñán, Noel
last_name: Blanco-Touriñán
- first_name: Antia
full_name: Rodriguez-Villalón, Antia
last_name: Rodriguez-Villalón
- first_name: Elisabeth
full_name: Truernit, Elisabeth
last_name: Truernit
citation:
ama: 'Bartusch K, Blanco-Touriñán N, Rodriguez-Villalón A, Truernit E. Monitoring
Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes. In:
Xylem. Vol 2722. 2nd ed. MIMB. New York, NY: Springer; 2023:3-15. doi:10.1007/978-1-0716-3477-6_1'
apa: 'Bartusch, K., Blanco-Touriñán, N., Rodriguez-Villalón, A., & Truernit,
E. (2023). Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using
Fluorescent Dyes. In Xylem (2nd ed., Vol. 2722, pp. 3–15). New York, NY:
Springer. https://doi.org/10.1007/978-1-0716-3477-6_1'
chicago: 'Bartusch, Kai, Noel Blanco-Touriñán, Antia Rodriguez-Villalón, and Elisabeth
Truernit. “Monitoring Xylem Transport in Arabidopsis Thaliana Seedlings Using
Fluorescent Dyes.” In Xylem, 2nd ed., 2722:3–15. MIMB. New York, NY: Springer,
2023. https://doi.org/10.1007/978-1-0716-3477-6_1.'
ieee: 'K. Bartusch, N. Blanco-Touriñán, A. Rodriguez-Villalón, and E. Truernit,
“Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent
Dyes,” in Xylem, 2nd ed., vol. 2722, New York, NY: Springer, 2023, pp.
3–15.'
ista: 'Bartusch K, Blanco-Touriñán N, Rodriguez-Villalón A, Truernit E. 2023.Monitoring
Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes. In:
Xylem. Methods in Molecular Biology, vol. 2722, 3–15.'
mla: Bartusch, Kai, et al. “Monitoring Xylem Transport in Arabidopsis Thaliana Seedlings
Using Fluorescent Dyes.” Xylem, 2nd ed., vol. 2722, Springer, 2023, pp.
3–15, doi:10.1007/978-1-0716-3477-6_1.
short: K. Bartusch, N. Blanco-Touriñán, A. Rodriguez-Villalón, E. Truernit, in:,
Xylem, 2nd ed., Springer, New York, NY, 2023, pp. 3–15.
date_created: 2026-10-13T11:15:03Z
date_published: 2023-10-29T00:00:00Z
date_updated: 2026-10-14T10:33:41Z
day: '29'
doi: 10.1007/978-1-0716-3477-6_1
edition: '2'
extern: '1'
external_id:
pmid:
- '37897596'
fulldoi: https://doi.org/10.1007/978-1-0716-3477-6_1
intvolume: ' 2722'
language:
- iso: eng
month: '10'
oa_version: None
page: 3-15
place: New York, NY
pmid: 1
publication: Xylem
publication_identifier:
eisbn:
- '9781071634776'
eissn:
- 1940-6029
isbn:
- '9781071634769'
issn:
- 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent
Dyes
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2722
year: '2023'
...
---
_id: '10268'
abstract:
- lang: eng
text: The analysis of dynamic cellular processes such as plant cytokinesis stands
and falls with live-cell time-lapse confocal imaging. Conventional approaches
to time-lapse imaging of cell division in Arabidopsis root tips are tedious and
have low throughput. Here, we describe a protocol for long-term time-lapse simultaneous
imaging of multiple root tips on a vertical-stage confocal microscope with automated
root tracking. We also provide modifications of the basic protocol to implement
this imaging method in the analysis of genetic, pharmacological or laser ablation
wounding-mediated experimental manipulations. Our method dramatically improves
the efficiency of cell division time-lapse imaging by increasing the throughput,
while reducing the person-hour requirements of such experiments.
acknowledged_ssus:
- _id: Bio
acknowledgement: We thank B. De Rybel for allowing M.G. to work on this manuscript
during a postdoc in his laboratory, and EMBO for supporting M.G. with a Long-Term
fellowship (ALTF 1005-2019) during this time. We acknowledge the service and support
by the Bioimaging Facility at IST Austria, and finally, we thank A. Mally for proofreading
and correcting the manuscript.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Lukas
full_name: Hörmayer, Lukas
id: 2EEE7A2A-F248-11E8-B48F-1D18A9856A87
last_name: Hörmayer
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
citation:
ama: 'Hörmayer L, Friml J, Glanc M. Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
In: Plant Cell Division. Vol 2382. MIMB. Humana Press; 2021:105-114. doi:10.1007/978-1-0716-1744-1_6'
apa: Hörmayer, L., Friml, J., & Glanc, M. (2021). Automated time-lapse imaging
and manipulation of cell divisions in Arabidopsis roots by vertical-stage confocal
microscopy. In Plant Cell Division (Vol. 2382, pp. 105–114). Humana Press.
https://doi.org/10.1007/978-1-0716-1744-1_6
chicago: Hörmayer, Lukas, Jiří Friml, and Matous Glanc. “Automated Time-Lapse Imaging
and Manipulation of Cell Divisions in Arabidopsis Roots by Vertical-Stage Confocal
Microscopy.” In Plant Cell Division, 2382:105–14. MIMB. Humana Press, 2021.
https://doi.org/10.1007/978-1-0716-1744-1_6.
ieee: L. Hörmayer, J. Friml, and M. Glanc, “Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy,”
in Plant Cell Division, vol. 2382, Humana Press, 2021, pp. 105–114.
ista: 'Hörmayer L, Friml J, Glanc M. 2021.Automated time-lapse imaging and manipulation
of cell divisions in Arabidopsis roots by vertical-stage confocal microscopy.
In: Plant Cell Division. Methods in Molecular Biology, vol. 2382, 105–114.'
mla: Hörmayer, Lukas, et al. “Automated Time-Lapse Imaging and Manipulation of Cell
Divisions in Arabidopsis Roots by Vertical-Stage Confocal Microscopy.” Plant
Cell Division, vol. 2382, Humana Press, 2021, pp. 105–14, doi:10.1007/978-1-0716-1744-1_6.
short: L. Hörmayer, J. Friml, M. Glanc, in:, Plant Cell Division, Humana Press,
2021, pp. 105–114.
date_created: 2021-11-11T10:03:30Z
date_published: 2021-10-28T00:00:00Z
date_updated: 2022-06-03T06:47:06Z
day: '28'
department:
- _id: JiFr
doi: 10.1007/978-1-0716-1744-1_6
external_id:
pmid:
- '34705235'
fulldoi: https://doi.org/10.1007/978-1-0716-1744-1_6
intvolume: ' 2382'
language:
- iso: eng
month: '10'
oa_version: None
page: 105-114
pmid: 1
publication: Plant Cell Division
publication_identifier:
eisbn:
- 978-1-0716-1744-1
eissn:
- 1940-6029
isbn:
- 978-1-0716-1743-4
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Automated time-lapse imaging and manipulation of cell divisions in Arabidopsis
roots by vertical-stage confocal microscopy
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2382
year: '2021'
...
---
_id: '9245'
abstract:
- lang: eng
text: Tissue morphogenesis is driven by mechanical forces triggering cell movements
and shape changes. Quantitatively measuring tension within tissues is of great
importance for understanding the role of mechanical signals acting on the cell
and tissue level during morphogenesis. Here we introduce laser ablation as a useful
tool to probe tissue tension within the granulosa layer, an epithelial monolayer
of somatic cells that surround the zebrafish female gamete during folliculogenesis.
We describe in detail how to isolate follicles, mount samples, perform laser surgery,
and analyze the data.
acknowledged_ssus:
- _id: Bio
- _id: PreCl
acknowledgement: We thank Prof. Masazumi Tada and Roland Dosch for providing transgenic
zebrafish lines, the Heisenberg lab for technical assistance and feedback on the
manuscript, and the Bioimaging and Fish facilities of IST Austria for continuous
support. This work was funded by an ERC advanced grant (MECSPEC to C.-P.H.).
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Peng
full_name: Xia, Peng
id: 4AB6C7D0-F248-11E8-B48F-1D18A9856A87
last_name: Xia
orcid: 0000-0002-5419-7756
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Xia P, Heisenberg C-PJ. Quantifying tissue tension in the granulosa layer
after laser surgery. In: Dosch R, ed. Germline Development in the Zebrafish.
Vol 2218. Humana Press; 2021:117-128. doi:10.1007/978-1-0716-0970-5_10'
apa: Xia, P., & Heisenberg, C.-P. J. (2021). Quantifying tissue tension in the
granulosa layer after laser surgery. In R. Dosch (Ed.), Germline Development
in the Zebrafish (Vol. 2218, pp. 117–128). Humana Press. https://doi.org/10.1007/978-1-0716-0970-5_10
chicago: Xia, Peng, and Carl-Philipp J Heisenberg. “Quantifying Tissue Tension in
the Granulosa Layer after Laser Surgery.” In Germline Development in the Zebrafish,
edited by Roland Dosch, 2218:117–28. Humana Press, 2021. https://doi.org/10.1007/978-1-0716-0970-5_10.
ieee: P. Xia and C.-P. J. Heisenberg, “Quantifying tissue tension in the granulosa
layer after laser surgery,” in Germline Development in the Zebrafish, vol.
2218, R. Dosch, Ed. Humana Press, 2021, pp. 117–128.
ista: 'Xia P, Heisenberg C-PJ. 2021.Quantifying tissue tension in the granulosa
layer after laser surgery. In: Germline Development in the Zebrafish. Methods
in Molecular Biology, vol. 2218, 117–128.'
mla: Xia, Peng, and Carl-Philipp J. Heisenberg. “Quantifying Tissue Tension in the
Granulosa Layer after Laser Surgery.” Germline Development in the Zebrafish,
edited by Roland Dosch, vol. 2218, Humana Press, 2021, pp. 117–28, doi:10.1007/978-1-0716-0970-5_10.
short: P. Xia, C.-P.J. Heisenberg, in:, R. Dosch (Ed.), Germline Development in
the Zebrafish, Humana Press, 2021, pp. 117–128.
corr_author: '1'
das_tickbox: '1'
date_created: 2021-03-14T23:01:34Z
date_published: 2021-02-20T00:00:00Z
date_updated: 2026-07-06T13:11:10Z
day: '20'
department:
- _id: CaHe
doi: 10.1007/978-1-0716-0970-5_10
ec_funded: 1
editor:
- first_name: Roland
full_name: Dosch, Roland
last_name: Dosch
external_id:
pmid:
- '33606227'
fulldoi: https://doi.org/10.1007/978-1-0716-0970-5_10
intvolume: ' 2218'
keyword:
- Tissue tension
- Morphogenesis
- Laser ablation
- Zebrafish folliculogenesis
- Granulosa cells
language:
- iso: eng
month: '02'
oa_version: None
page: 117-128
pmid: 1
project:
- _id: 260F1432-B435-11E9-9278-68D0E5697425
call_identifier: H2020
grant_number: '742573'
name: Interaction and feedback between cell mechanics and fate specification in
vertebrate gastrulation
publication: Germline Development in the Zebrafish
publication_identifier:
eisbn:
- 978-1-0716-0970-5
eissn:
- 1940-6029
isbn:
- 978-1-0716-0969-9
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
status: public
title: Quantifying tissue tension in the granulosa layer after laser surgery
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2218
year: '2021'
...
---
_id: '8173'
abstract:
- lang: eng
text: Understanding how the activity of membrane receptors and cellular signaling
pathways shapes cell behavior is of fundamental interest in basic and applied
research. Reengineering receptors to react to light instead of their cognate ligands
allows for generating defined signaling inputs with high spatial and temporal
precision and facilitates the dissection of complex signaling networks. Here,
we describe fundamental considerations in the design of light-regulated receptor
tyrosine kinases (Opto-RTKs) and appropriate control experiments. We also introduce
methods for transient receptor expression in HEK293 cells, quantitative assessment
of signaling activity in reporter gene assays, semiquantitative assessment of
(in)activation time courses through Western blot (WB) analysis, and easy to implement
light stimulation hardware.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Stephanie
full_name: Kainrath, Stephanie
id: 32CFBA64-F248-11E8-B48F-1D18A9856A87
last_name: Kainrath
orcid: 0000-0002-6709-2195
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
citation:
ama: 'Kainrath S, Janovjak HL. Design and application of light-regulated receptor
tyrosine kinases. In: Niopek D, ed. Photoswitching Proteins. Vol 2173.
MIMB. Springer Nature; 2020:233-246. doi:10.1007/978-1-0716-0755-8_16'
apa: Kainrath, S., & Janovjak, H. L. (2020). Design and application of light-regulated
receptor tyrosine kinases. In D. Niopek (Ed.), Photoswitching Proteins
(Vol. 2173, pp. 233–246). Springer Nature. https://doi.org/10.1007/978-1-0716-0755-8_16
chicago: Kainrath, Stephanie, and Harald L Janovjak. “Design and Application of
Light-Regulated Receptor Tyrosine Kinases.” In Photoswitching Proteins,
edited by Dominik Niopek, 2173:233–46. MIMB. Springer Nature, 2020. https://doi.org/10.1007/978-1-0716-0755-8_16.
ieee: S. Kainrath and H. L. Janovjak, “Design and application of light-regulated
receptor tyrosine kinases,” in Photoswitching Proteins, vol. 2173, D. Niopek,
Ed. Springer Nature, 2020, pp. 233–246.
ista: 'Kainrath S, Janovjak HL. 2020.Design and application of light-regulated receptor
tyrosine kinases. In: Photoswitching Proteins. Methods in Molecular Biology, vol.
2173, 233–246.'
mla: Kainrath, Stephanie, and Harald L. Janovjak. “Design and Application of Light-Regulated
Receptor Tyrosine Kinases.” Photoswitching Proteins, edited by Dominik
Niopek, vol. 2173, Springer Nature, 2020, pp. 233–46, doi:10.1007/978-1-0716-0755-8_16.
short: S. Kainrath, H.L. Janovjak, in:, D. Niopek (Ed.), Photoswitching Proteins,
Springer Nature, 2020, pp. 233–246.
date_created: 2020-07-26T22:01:03Z
date_published: 2020-07-11T00:00:00Z
date_updated: 2026-04-16T09:22:45Z
day: '11'
department:
- _id: CaGu
doi: 10.1007/978-1-0716-0755-8_16
editor:
- first_name: Dominik
full_name: Niopek, Dominik
last_name: Niopek
external_id:
pmid:
- '32651922'
fulldoi: https://doi.org/10.1007/978-1-0716-0755-8_16
intvolume: ' 2173'
language:
- iso: eng
month: '07'
oa_version: None
page: 233-246
pmid: 1
publication: Photoswitching Proteins
publication_identifier:
eisbn:
- '9781071607558'
eissn:
- 1940-6029
isbn:
- '9781071607541'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
scopus_import: '1'
series_title: MIMB
status: public
title: Design and application of light-regulated receptor tyrosine kinases
type: book_chapter
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 2173
year: '2020'
...
---
_id: '11847'
abstract:
- lang: eng
text: This paper serves as a user guide to the Vienna graph clustering framework.
We review our general memetic algorithm, VieClus, to tackle the graph clustering
problem. A key component of our contribution are natural recombine operators that
employ ensemble clusterings as well as multi-level techniques. Lastly, we combine
these techniques with a scalable communication protocol, producing a system that
is able to compute high-quality solutions in a short amount of time. After giving
a description of the algorithms employed, we establish the connection of the graph
clustering problem to protein–protein interaction networks and moreover give a
description on how the software can be used, what file formats are expected, and
how this can be used to find functional groups in protein–protein interaction
networks.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Sonja
full_name: Biedermann, Sonja
last_name: Biedermann
- first_name: Monika H
full_name: Henzinger, Monika H
id: 540c9bbd-f2de-11ec-812d-d04a5be85630
last_name: Henzinger
orcid: 0000-0002-5008-6530
- first_name: Christian
full_name: Schulz, Christian
last_name: Schulz
- first_name: Bernhard
full_name: Schuster, Bernhard
last_name: Schuster
citation:
ama: 'Biedermann S, Henzinger M, Schulz C, Schuster B. Vienna Graph Clustering.
In: Canzar S, Rojas Ringeling F, eds. Protein-Protein Interaction Networks.
Vol 2074. MIMB. Springer Nature; 2019:215–231. doi:10.1007/978-1-4939-9873-9_16'
apa: Biedermann, S., Henzinger, M., Schulz, C., & Schuster, B. (2019). Vienna
Graph Clustering. In S. Canzar & F. Rojas Ringeling (Eds.), Protein-Protein
Interaction Networks (Vol. 2074, pp. 215–231). Springer Nature. https://doi.org/10.1007/978-1-4939-9873-9_16
chicago: Biedermann, Sonja, Monika Henzinger, Christian Schulz, and Bernhard Schuster.
“Vienna Graph Clustering.” In Protein-Protein Interaction Networks, edited
by Stefan Canzar and Francisca Rojas Ringeling, 2074:215–231. MIMB. Springer Nature,
2019. https://doi.org/10.1007/978-1-4939-9873-9_16.
ieee: S. Biedermann, M. Henzinger, C. Schulz, and B. Schuster, “Vienna Graph Clustering,”
in Protein-Protein Interaction Networks, vol. 2074, S. Canzar and F. Rojas
Ringeling, Eds. Springer Nature, 2019, pp. 215–231.
ista: 'Biedermann S, Henzinger M, Schulz C, Schuster B. 2019.Vienna Graph Clustering.
In: Protein-Protein Interaction Networks. Methods in Molecular Biology, vol. 2074,
215–231.'
mla: Biedermann, Sonja, et al. “Vienna Graph Clustering.” Protein-Protein Interaction
Networks, edited by Stefan Canzar and Francisca Rojas Ringeling, vol. 2074,
Springer Nature, 2019, pp. 215–231, doi:10.1007/978-1-4939-9873-9_16.
short: S. Biedermann, M. Henzinger, C. Schulz, B. Schuster, in:, S. Canzar, F. Rojas
Ringeling (Eds.), Protein-Protein Interaction Networks, Springer Nature, 2019,
pp. 215–231.
date_created: 2022-08-16T06:54:48Z
date_published: 2019-10-04T00:00:00Z
date_updated: 2024-11-06T12:17:08Z
day: '04'
doi: 10.1007/978-1-4939-9873-9_16
editor:
- first_name: Stefan
full_name: Canzar, Stefan
last_name: Canzar
- first_name: Francisca
full_name: Rojas Ringeling, Francisca
last_name: Rojas Ringeling
extern: '1'
external_id:
pmid:
- '31583641'
fulldoi: https://doi.org/10.1007/978-1-4939-9873-9_16
intvolume: ' 2074'
language:
- iso: eng
month: '10'
oa_version: None
page: 215–231
pmid: 1
publication: Protein-Protein Interaction Networks
publication_identifier:
eisbn:
- '9781493998739'
eissn:
- 1940-6029
isbn:
- '9781493998722'
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Vienna Graph Clustering
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 2074
year: '2019'
...
---
_id: '37'
abstract:
- lang: eng
text: Developmental processes are inherently dynamic and understanding them requires
quantitative measurements of gene and protein expression levels in space and time.
While live imaging is a powerful approach for obtaining such data, it is still
a challenge to apply it over long periods of time to large tissues, such as the
embryonic spinal cord in mouse and chick. Nevertheless, dynamics of gene expression
and signaling activity patterns in this organ can be studied by collecting tissue
sections at different developmental stages. In combination with immunohistochemistry,
this allows for measuring the levels of multiple developmental regulators in a
quantitative manner with high spatiotemporal resolution. The mean protein expression
levels over time, as well as embryo-to-embryo variability can be analyzed. A key
aspect of the approach is the ability to compare protein levels across different
samples. This requires a number of considerations in sample preparation, imaging
and data analysis. Here we present a protocol for obtaining time course data of
dorsoventral expression patterns from mouse and chick neural tube in the first
3 days of neural tube development. The described workflow starts from embryo dissection
and ends with a processed dataset. Software scripts for data analysis are included.
The protocol is adaptable and instructions that allow the user to modify different
steps are provided. Thus, the procedure can be altered for analysis of time-lapse
images and applied to systems other than the neural tube.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Marcin P
full_name: Zagórski, Marcin P
id: 343DA0DC-F248-11E8-B48F-1D18A9856A87
last_name: Zagórski
orcid: 0000-0001-7896-7762
- first_name: Anna
full_name: Kicheva, Anna
id: 3959A2A0-F248-11E8-B48F-1D18A9856A87
last_name: Kicheva
orcid: 0000-0003-4509-4998
citation:
ama: 'Zagórski MP, Kicheva A. Measuring dorsoventral pattern and morphogen signaling
profiles in the growing neural tube. In: Morphogen Gradients . Vol 1863.
MIMB. Springer Nature; 2018:47-63. doi:10.1007/978-1-4939-8772-6_4'
apa: Zagórski, M. P., & Kicheva, A. (2018). Measuring dorsoventral pattern and
morphogen signaling profiles in the growing neural tube. In Morphogen Gradients
(Vol. 1863, pp. 47–63). Springer Nature. https://doi.org/10.1007/978-1-4939-8772-6_4
chicago: Zagórski, Marcin P, and Anna Kicheva. “Measuring Dorsoventral Pattern and
Morphogen Signaling Profiles in the Growing Neural Tube.” In Morphogen Gradients
, 1863:47–63. MIMB. Springer Nature, 2018. https://doi.org/10.1007/978-1-4939-8772-6_4.
ieee: M. P. Zagórski and A. Kicheva, “Measuring dorsoventral pattern and morphogen
signaling profiles in the growing neural tube,” in Morphogen Gradients ,
vol. 1863, Springer Nature, 2018, pp. 47–63.
ista: 'Zagórski MP, Kicheva A. 2018.Measuring dorsoventral pattern and morphogen
signaling profiles in the growing neural tube. In: Morphogen Gradients . Methods
in Molecular Biology, vol. 1863, 47–63.'
mla: Zagórski, Marcin P., and Anna Kicheva. “Measuring Dorsoventral Pattern and
Morphogen Signaling Profiles in the Growing Neural Tube.” Morphogen Gradients
, vol. 1863, Springer Nature, 2018, pp. 47–63, doi:10.1007/978-1-4939-8772-6_4.
short: M.P. Zagórski, A. Kicheva, in:, Morphogen Gradients , Springer Nature, 2018,
pp. 47–63.
date_created: 2018-12-11T11:44:17Z
date_published: 2018-10-16T00:00:00Z
date_updated: 2025-04-14T07:27:29Z
day: '16'
ddc:
- '570'
department:
- _id: AnKi
doi: 10.1007/978-1-4939-8772-6_4
ec_funded: 1
file:
- access_level: open_access
checksum: 2a97d0649fdcfcf1bdca7c8ad1dce71b
content_type: application/pdf
creator: dernst
date_created: 2020-10-13T14:20:37Z
date_updated: 2020-10-13T14:20:37Z
file_id: '8656'
file_name: 2018_MIMB_Zagorski.pdf
file_size: 4906815
relation: main_file
success: 1
file_date_updated: 2020-10-13T14:20:37Z
fulldoi: https://doi.org/10.1007/978-1-4939-8772-6_4
has_accepted_license: '1'
intvolume: ' 1863'
language:
- iso: eng
month: '10'
oa: 1
oa_version: Submitted Version
page: 47 - 63
project:
- _id: B6FC0238-B512-11E9-945C-1524E6697425
call_identifier: H2020
grant_number: '680037'
name: Coordination of Patterning And Growth In the Spinal Cord
publication: 'Morphogen Gradients '
publication_identifier:
isbn:
- 978-1-4939-8771-9
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
publist_id: '8018'
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Measuring dorsoventral pattern and morphogen signaling profiles in the growing
neural tube
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1863
year: '2018'
...
---
_id: '408'
abstract:
- lang: eng
text: Adventitious roots (AR) are de novo formed roots that emerge from any part
of the plant or from callus in tissue culture, except root tissue. The plant tissue
origin and the method by which they are induced determine the physiological properties
of emerged ARs. Hence, a standard method encompassing all types of AR does not
exist. Here we describe a method for the induction and analysis of AR that emerge
from the etiolated hypocotyl of dicot plants. The hypocotyl is formed during embryogenesis
and shows a determined developmental pattern which usually does not involve AR
formation. However, the hypocotyl shows propensity to form de novo roots under
specific circumstances such as removal of the root system, high humidity or flooding,
or during de-etiolation. The hypocotyl AR emerge from a pericycle-like cell layer
surrounding the vascular tissue of the central cylinder, which is reminiscent
to the developmental program of lateral roots. Here we propose an easy protocol
for in vitro hypocotyl AR induction from etiolated Arabidopsis seedlings.
alternative_title:
- MIMB
article_processing_charge: No
author:
- first_name: Hoang
full_name: Trinh, Hoang
last_name: Trinh
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Danny
full_name: Geelen, Danny
last_name: Geelen
citation:
ama: 'Trinh H, Verstraeten I, Geelen D. In vitro assay for induction of adventitious
rooting on intact arabidopsis hypocotyls. In: Root Development . Vol 1761.
Springer Nature; 2018:95-102. doi:10.1007/978-1-4939-7747-5_7'
apa: Trinh, H., Verstraeten, I., & Geelen, D. (2018). In vitro assay for induction
of adventitious rooting on intact arabidopsis hypocotyls. In Root Development
(Vol. 1761, pp. 95–102). Springer Nature. https://doi.org/10.1007/978-1-4939-7747-5_7
chicago: Trinh, Hoang, Inge Verstraeten, and Danny Geelen. “In Vitro Assay for Induction
of Adventitious Rooting on Intact Arabidopsis Hypocotyls.” In Root Development
, 1761:95–102. Springer Nature, 2018. https://doi.org/10.1007/978-1-4939-7747-5_7.
ieee: H. Trinh, I. Verstraeten, and D. Geelen, “In vitro assay for induction of
adventitious rooting on intact arabidopsis hypocotyls,” in Root Development
, vol. 1761, Springer Nature, 2018, pp. 95–102.
ista: 'Trinh H, Verstraeten I, Geelen D. 2018.In vitro assay for induction of adventitious
rooting on intact arabidopsis hypocotyls. In: Root Development . MIMB, vol. 1761,
95–102.'
mla: Trinh, Hoang, et al. “In Vitro Assay for Induction of Adventitious Rooting
on Intact Arabidopsis Hypocotyls.” Root Development , vol. 1761, Springer
Nature, 2018, pp. 95–102, doi:10.1007/978-1-4939-7747-5_7.
short: H. Trinh, I. Verstraeten, D. Geelen, in:, Root Development , Springer Nature,
2018, pp. 95–102.
date_created: 2018-12-11T11:46:18Z
date_published: 2018-03-01T00:00:00Z
date_updated: 2021-01-12T07:54:21Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-1-4939-7747-5_7
external_id:
pmid:
- '29525951'
fulldoi: https://doi.org/10.1007/978-1-4939-7747-5_7
intvolume: ' 1761'
language:
- iso: eng
month: '03'
oa_version: None
page: 95 - 102
pmid: 1
publication: 'Root Development '
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer Nature
publist_id: '7421'
quality_controlled: '1'
scopus_import: '1'
status: public
title: In vitro assay for induction of adventitious rooting on intact arabidopsis
hypocotyls
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1761
year: '2018'
...
---
_id: '8450'
abstract:
- lang: eng
text: Methyl groups are very useful probes of structure, dynamics, and interactions
in protein NMR spectroscopy. In particular, methyl-directed experiments provide
high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking
environment. In this chapter, we discuss the approach for labeling methyl groups
in E. coli-based protein expression, as exemplified with the mitochondrial carrier
GGC.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Vilius
full_name: Kurauskas, Vilius
last_name: Kurauskas
- first_name: Paul
full_name: Schanda, Paul
id: 7B541462-FAF6-11E9-A490-E8DFE5697425
last_name: Schanda
orcid: 0000-0002-9350-7606
- first_name: Remy
full_name: Sounier, Remy
last_name: Sounier
citation:
ama: 'Kurauskas V, Schanda P, Sounier R. Methyl-specific isotope labeling strategies
for NMR studies of membrane proteins. In: Membrane Protein Structure and Function
Characterization. Vol 1635. Springer Nature; 2017:109-123. doi:10.1007/978-1-4939-7151-0_6'
apa: Kurauskas, V., Schanda, P., & Sounier, R. (2017). Methyl-specific isotope
labeling strategies for NMR studies of membrane proteins. In Membrane protein
structure and function characterization (Vol. 1635, pp. 109–123). Springer
Nature. https://doi.org/10.1007/978-1-4939-7151-0_6
chicago: Kurauskas, Vilius, Paul Schanda, and Remy Sounier. “Methyl-Specific Isotope
Labeling Strategies for NMR Studies of Membrane Proteins.” In Membrane Protein
Structure and Function Characterization, 1635:109–23. Springer Nature, 2017.
https://doi.org/10.1007/978-1-4939-7151-0_6.
ieee: V. Kurauskas, P. Schanda, and R. Sounier, “Methyl-specific isotope labeling
strategies for NMR studies of membrane proteins,” in Membrane protein structure
and function characterization, vol. 1635, Springer Nature, 2017, pp. 109–123.
ista: 'Kurauskas V, Schanda P, Sounier R. 2017.Methyl-specific isotope labeling
strategies for NMR studies of membrane proteins. In: Membrane protein structure
and function characterization. Methods in Molecular Biology, vol. 1635, 109–123.'
mla: Kurauskas, Vilius, et al. “Methyl-Specific Isotope Labeling Strategies for
NMR Studies of Membrane Proteins.” Membrane Protein Structure and Function
Characterization, vol. 1635, Springer Nature, 2017, pp. 109–23, doi:10.1007/978-1-4939-7151-0_6.
short: V. Kurauskas, P. Schanda, R. Sounier, in:, Membrane Protein Structure and
Function Characterization, Springer Nature, 2017, pp. 109–123.
date_created: 2020-09-18T10:06:44Z
date_published: 2017-07-29T00:00:00Z
date_updated: 2022-08-26T09:14:20Z
day: '29'
doi: 10.1007/978-1-4939-7151-0_6
extern: '1'
fulldoi: https://doi.org/10.1007/978-1-4939-7151-0_6
intvolume: ' 1635'
language:
- iso: eng
month: '07'
oa_version: None
page: 109-123
publication: Membrane protein structure and function characterization
publication_identifier:
isbn:
- '9781493971497'
- '9781493971510'
issn:
- 1064-3745
- 1940-6029
publication_status: published
publisher: Springer Nature
quality_controlled: '1'
status: public
title: Methyl-specific isotope labeling strategies for NMR studies of membrane proteins
type: book_chapter
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 1635
year: '2017'
...
---
_id: '957'
abstract:
- lang: eng
text: Small molecule biosensors based on Forster resonance energy transfer (FRET)
enable small molecule signaling to be monitored with high spatial and temporal
resolution in complex cellular environments. FRET sensors can be constructed by
fusing a pair of fluorescent proteins to a suitable recognition domain, such as
a member of the solute-binding protein (SBP) superfamily. However, naturally occurring
SBPs may be unsuitable for incorporation into FRET sensors due to their low thermostability,
which may preclude imaging under physiological conditions, or because the positions
of their N- and C-termini may be suboptimal for fusion of fluorescent proteins,
which may limit the dynamic range of the resulting sensors. Here, we show how
these problems can be overcome using ancestral protein reconstruction and circular
permutation. Ancestral protein reconstruction, used as a protein engineering strategy,
leverages phylogenetic information to improve the thermostability of proteins,
while circular permutation enables the termini of an SBP to be repositioned to
maximize the dynamic range of the resulting FRET sensor. We also provide a protocol
for cloning the engineered SBPs into FRET sensor constructs using Golden Gate
assembly and discuss considerations for in situ characterization of the FRET sensors.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Ben
full_name: Clifton, Ben
last_name: Clifton
- first_name: Jason
full_name: Whitfield, Jason
last_name: Whitfield
- first_name: Inmaculada
full_name: Sanchez Romero, Inmaculada
id: 3D9C5D30-F248-11E8-B48F-1D18A9856A87
last_name: Sanchez Romero
- first_name: Michel
full_name: Herde, Michel
last_name: Herde
- first_name: Christian
full_name: Henneberger, Christian
last_name: Henneberger
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
- first_name: Colin
full_name: Jackson, Colin
last_name: Jackson
citation:
ama: 'Clifton B, Whitfield J, Sanchez-Romero I, et al. Ancestral protein reconstruction
and circular permutation for improving the stability and dynamic range of FRET
sensors. In: Stein V, ed. Synthetic Protein Switches. Vol 1596. Synthetic
Protein Switches. Springer; 2017:71-87. doi:10.1007/978-1-4939-6940-1_5'
apa: Clifton, B., Whitfield, J., Sanchez-Romero, I., Herde, M., Henneberger, C.,
Janovjak, H. L., & Jackson, C. (2017). Ancestral protein reconstruction and
circular permutation for improving the stability and dynamic range of FRET sensors.
In V. Stein (Ed.), Synthetic Protein Switches (Vol. 1596, pp. 71–87). Springer.
https://doi.org/10.1007/978-1-4939-6940-1_5
chicago: Clifton, Ben, Jason Whitfield, Inmaculada Sanchez-Romero, Michel Herde,
Christian Henneberger, Harald L Janovjak, and Colin Jackson. “Ancestral Protein
Reconstruction and Circular Permutation for Improving the Stability and Dynamic
Range of FRET Sensors.” In Synthetic Protein Switches, edited by Viktor
Stein, 1596:71–87. Synthetic Protein Switches. Springer, 2017. https://doi.org/10.1007/978-1-4939-6940-1_5.
ieee: B. Clifton et al., “Ancestral protein reconstruction and circular permutation
for improving the stability and dynamic range of FRET sensors,” in Synthetic
Protein Switches, vol. 1596, V. Stein, Ed. Springer, 2017, pp. 71–87.
ista: 'Clifton B, Whitfield J, Sanchez-Romero I, Herde M, Henneberger C, Janovjak
HL, Jackson C. 2017.Ancestral protein reconstruction and circular permutation
for improving the stability and dynamic range of FRET sensors. In: Synthetic Protein
Switches. Methods in Molecular Biology, vol. 1596, 71–87.'
mla: Clifton, Ben, et al. “Ancestral Protein Reconstruction and Circular Permutation
for Improving the Stability and Dynamic Range of FRET Sensors.” Synthetic Protein
Switches, edited by Viktor Stein, vol. 1596, Springer, 2017, pp. 71–87, doi:10.1007/978-1-4939-6940-1_5.
short: B. Clifton, J. Whitfield, I. Sanchez-Romero, M. Herde, C. Henneberger, H.L.
Janovjak, C. Jackson, in:, V. Stein (Ed.), Synthetic Protein Switches, Springer,
2017, pp. 71–87.
date_created: 2018-12-11T11:49:24Z
date_published: 2017-03-15T00:00:00Z
date_updated: 2025-07-10T12:01:52Z
day: '15'
department:
- _id: HaJa
doi: 10.1007/978-1-4939-6940-1_5
editor:
- first_name: Viktor
full_name: Stein, Viktor
last_name: Stein
fulldoi: https://doi.org/10.1007/978-1-4939-6940-1_5
intvolume: ' 1596'
language:
- iso: eng
month: '03'
oa_version: None
page: 71 - 87
project:
- _id: 255BFFFA-B435-11E9-9278-68D0E5697425
grant_number: RGY0084/2012
name: In situ real-time imaging of neurotransmitter signaling using designer optical
sensors
publication: Synthetic Protein Switches
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer
publist_id: '6451'
quality_controlled: '1'
scopus_import: '1'
series_title: Synthetic Protein Switches
status: public
title: Ancestral protein reconstruction and circular permutation for improving the
stability and dynamic range of FRET sensors
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1596
year: '2017'
...
---
_id: '958'
abstract:
- lang: eng
text: Biosensors that exploit Forster resonance energy transfer (FRET) can be used
to visualize biological and physiological processes and are capable of providing
detailed information in both spatial and temporal dimensions. In a FRET-based
biosensor, substrate binding is associated with a change in the relative positions
of two fluorophores, leading to a change in FRET efficiency that may be observed
in the fluorescence spectrum. As a result, their design requires a ligand-binding
protein that exhibits a conformational change upon binding. However, not all ligand-binding
proteins produce responsive sensors upon conjugation to fluorescent proteins or
dyes, and identifying the optimum locations for the fluorophores often involves
labor-intensive iterative design or high-throughput screening. Combining the genetic
fusion of a fluorescent protein to the ligand-binding protein with site-specific
covalent attachment of a fluorescent dye can allow fine control over the positions
of the two fluorophores, allowing the construction of very sensitive sensors.
This relies upon the accurate prediction of the locations of the two fluorophores
in bound and unbound states. In this chapter, we describe a method for computational
identification of dye-attachment sites that allows the use of cysteine modification
to attach synthetic dyes that can be paired with a fluorescent protein for the
purposes of creating FRET sensors.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Joshua
full_name: Mitchell, Joshua
last_name: Mitchell
- first_name: William
full_name: Zhang, William
last_name: Zhang
- first_name: Michel
full_name: Herde, Michel
last_name: Herde
- first_name: Christian
full_name: Henneberger, Christian
last_name: Henneberger
- first_name: Harald L
full_name: Janovjak, Harald L
id: 33BA6C30-F248-11E8-B48F-1D18A9856A87
last_name: Janovjak
orcid: 0000-0002-8023-9315
- first_name: Megan
full_name: O'Mara, Megan
last_name: O'Mara
- first_name: Colin
full_name: Jackson, Colin
last_name: Jackson
citation:
ama: 'Mitchell J, Zhang W, Herde M, et al. Method for developing optical sensors
using a synthetic dye fluorescent protein FRET pair and computational modeling
and assessment. In: Stein V, ed. Synthetic Protein Switches. Vol 1596.
Synthetic Protein Switches. Springer; 2017:89-99. doi:10.1007/978-1-4939-6940-1_6'
apa: Mitchell, J., Zhang, W., Herde, M., Henneberger, C., Janovjak, H. L., O’Mara,
M., & Jackson, C. (2017). Method for developing optical sensors using a synthetic
dye fluorescent protein FRET pair and computational modeling and assessment. In
V. Stein (Ed.), Synthetic Protein Switches (Vol. 1596, pp. 89–99). Springer.
https://doi.org/10.1007/978-1-4939-6940-1_6
chicago: Mitchell, Joshua, William Zhang, Michel Herde, Christian Henneberger, Harald
L Janovjak, Megan O’Mara, and Colin Jackson. “Method for Developing Optical Sensors
Using a Synthetic Dye Fluorescent Protein FRET Pair and Computational Modeling
and Assessment.” In Synthetic Protein Switches, edited by Viktor Stein,
1596:89–99. Synthetic Protein Switches. Springer, 2017. https://doi.org/10.1007/978-1-4939-6940-1_6.
ieee: J. Mitchell et al., “Method for developing optical sensors using a
synthetic dye fluorescent protein FRET pair and computational modeling and assessment,”
in Synthetic Protein Switches, vol. 1596, V. Stein, Ed. Springer, 2017,
pp. 89–99.
ista: 'Mitchell J, Zhang W, Herde M, Henneberger C, Janovjak HL, O’Mara M, Jackson
C. 2017.Method for developing optical sensors using a synthetic dye fluorescent
protein FRET pair and computational modeling and assessment. In: Synthetic Protein
Switches. Methods in Molecular Biology, vol. 1596, 89–99.'
mla: Mitchell, Joshua, et al. “Method for Developing Optical Sensors Using a Synthetic
Dye Fluorescent Protein FRET Pair and Computational Modeling and Assessment.”
Synthetic Protein Switches, edited by Viktor Stein, vol. 1596, Springer,
2017, pp. 89–99, doi:10.1007/978-1-4939-6940-1_6.
short: J. Mitchell, W. Zhang, M. Herde, C. Henneberger, H.L. Janovjak, M. O’Mara,
C. Jackson, in:, V. Stein (Ed.), Synthetic Protein Switches, Springer, 2017, pp.
89–99.
date_created: 2018-12-11T11:49:24Z
date_published: 2017-05-15T00:00:00Z
date_updated: 2025-07-10T12:01:54Z
day: '15'
department:
- _id: HaJa
doi: 10.1007/978-1-4939-6940-1_6
editor:
- first_name: Viktor
full_name: Stein, Viktor
last_name: Stein
fulldoi: https://doi.org/10.1007/978-1-4939-6940-1_6
intvolume: ' 1596'
language:
- iso: eng
month: '05'
oa_version: None
page: 89 - 99
publication: Synthetic Protein Switches
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer
publist_id: '6450'
quality_controlled: '1'
scopus_import: '1'
series_title: Synthetic Protein Switches
status: public
title: Method for developing optical sensors using a synthetic dye fluorescent protein
FRET pair and computational modeling and assessment
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1596
year: '2017'
...
---
_id: '1024'
abstract:
- lang: eng
text: The history of auxin and cytokinin biology including the initial discoveries
by father–son duo Charles Darwin and Francis Darwin (1880), and Gottlieb Haberlandt
(1919) is a beautiful demonstration of unceasing continuity of research. Novel
findings are integrated into existing hypotheses and models and deepen our understanding
of biological principles. At the same time new questions are triggered and hand
to hand with this new methodologies are developed to address these new challenges.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Andrej
full_name: Hurny, Andrej
id: 4DC4AF46-F248-11E8-B48F-1D18A9856A87
last_name: Hurny
orcid: 0000-0003-3638-1426
- first_name: Eva
full_name: Benková, Eva
id: 38F4F166-F248-11E8-B48F-1D18A9856A87
last_name: Benková
orcid: 0000-0002-8510-9739
citation:
ama: Hurny A, Benková E. Methodological advances in auxin and cytokinin biology.
Auxins and Cytokinins in Plant Biology. 2017;1569:1-29. doi:10.1007/978-1-4939-6831-2_1
apa: Hurny, A., & Benková, E. (2017). Methodological advances in auxin and cytokinin
biology. Auxins and Cytokinins in Plant Biology. Springer. https://doi.org/10.1007/978-1-4939-6831-2_1
chicago: Hurny, Andrej, and Eva Benková. “Methodological Advances in Auxin and Cytokinin
Biology.” Auxins and Cytokinins in Plant Biology. Springer, 2017. https://doi.org/10.1007/978-1-4939-6831-2_1.
ieee: A. Hurny and E. Benková, “Methodological advances in auxin and cytokinin biology,”
Auxins and Cytokinins in Plant Biology, vol. 1569. Springer, pp. 1–29,
2017.
ista: Hurny A, Benková E. 2017. Methodological advances in auxin and cytokinin biology.
Auxins and Cytokinins in Plant Biology. 1569, 1–29.
mla: Hurny, Andrej, and Eva Benková. “Methodological Advances in Auxin and Cytokinin
Biology.” Auxins and Cytokinins in Plant Biology, vol. 1569, Springer,
2017, pp. 1–29, doi:10.1007/978-1-4939-6831-2_1.
short: A. Hurny, E. Benková, Auxins and Cytokinins in Plant Biology 1569 (2017)
1–29.
corr_author: '1'
date_created: 2018-12-11T11:49:45Z
date_published: 2017-03-17T00:00:00Z
date_updated: 2026-11-11T23:31:00Z
day: '17'
ddc:
- '575'
department:
- _id: EvBe
doi: 10.1007/978-1-4939-6831-2_1
file:
- access_level: open_access
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:14:18Z
date_updated: 2019-10-15T07:47:05Z
file_id: '5068'
file_name: IST-2018-1019-v1+1_Hurny_MethodsMolBiol_2017.pdf
file_size: 840646
relation: main_file
file_date_updated: 2019-10-15T07:47:05Z
fulldoi: https://doi.org/10.1007/978-1-4939-6831-2_1
has_accepted_license: '1'
intvolume: ' 1569'
language:
- iso: eng
month: '03'
oa: 1
oa_version: Submitted Version
page: 1 - 29
project:
- _id: 2542D156-B435-11E9-9278-68D0E5697425
call_identifier: FWF
grant_number: I 1774-B16
name: Hormone cross-talk drives nutrient dependent plant development
publication: Auxins and Cytokinins in Plant Biology
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer
publist_id: '6369'
pubrep_id: '1019'
quality_controlled: '1'
related_material:
record:
- id: '539'
relation: dissertation_contains
status: public
scopus_import: '1'
status: public
title: Methodological advances in auxin and cytokinin biology
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1569
year: '2017'
...
---
_id: '2245'
abstract:
- lang: eng
text: 'Exogenous application of biologically important molecules for plant growth
promotion and/or regulation is very common both in plant research and horticulture.
Plant hormones such as auxins and cytokinins are classes of compounds which are
often applied exogenously. Nevertheless, plants possess a well-established machinery
to regulate the active pool of exogenously applied compounds by converting them
to metabolites and conjugates. Consequently, it is often very useful to know the
in vivo status of applied compounds to connect them with some of the regulatory
events in plant developmental processes. The in vivo status of applied compounds
can be measured by incubating plants with radiolabeled compounds, followed by
extraction, purification, and HPLC metabolic profiling of plant extracts. Recently
we have used this method to characterize the intracellularly localized PIN protein,
PIN5. Here we explain the method in detail, with a focus on general application. '
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Sibu
full_name: Simon, Sibu
id: 4542EF9A-F248-11E8-B48F-1D18A9856A87
last_name: Simon
orcid: 0000-0002-1998-6741
- first_name: Petr
full_name: Skůpa, Petr
last_name: Skůpa
- first_name: Petre
full_name: Dobrev, Petre
last_name: Dobrev
- first_name: Jan
full_name: Petrášek, Jan
last_name: Petrášek
- first_name: Eva
full_name: Zažímalová, Eva
last_name: Zažímalová
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: 'Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. Analyzing the
in vivo status of exogenously applied auxins: A HPLC-based method to characterize
the intracellularly localized auxin transporters. In: Hicks G, Robert S, eds.
Plant Chemical Genomics. Vol 1056. Methods in Molecular Biology. Springer;
2014:255-264. doi:10.1007/978-1-62703-592-7_23'
apa: 'Simon, S., Skůpa, P., Dobrev, P., Petrášek, J., Zažímalová, E., & Friml,
J. (2014). Analyzing the in vivo status of exogenously applied auxins: A HPLC-based
method to characterize the intracellularly localized auxin transporters. In G.
Hicks & S. Robert (Eds.), Plant Chemical Genomics (Vol. 1056, pp. 255–264).
Springer. https://doi.org/10.1007/978-1-62703-592-7_23'
chicago: 'Simon, Sibu, Petr Skůpa, Petre Dobrev, Jan Petrášek, Eva Zažímalová, and
Jiří Friml. “Analyzing the in Vivo Status of Exogenously Applied Auxins: A HPLC-Based
Method to Characterize the Intracellularly Localized Auxin Transporters.” In Plant
Chemical Genomics, edited by Glenn Hicks and Stéphanie Robert, 1056:255–64.
Methods in Molecular Biology. Springer, 2014. https://doi.org/10.1007/978-1-62703-592-7_23.'
ieee: 'S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, and J. Friml,
“Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method
to characterize the intracellularly localized auxin transporters,” in Plant
Chemical Genomics, vol. 1056, G. Hicks and S. Robert, Eds. Springer, 2014,
pp. 255–264.'
ista: 'Simon S, Skůpa P, Dobrev P, Petrášek J, Zažímalová E, Friml J. 2014.Analyzing
the in vivo status of exogenously applied auxins: A HPLC-based method to characterize
the intracellularly localized auxin transporters. In: Plant Chemical Genomics.
Methods in Molecular Biology, vol. 1056, 255–264.'
mla: 'Simon, Sibu, et al. “Analyzing the in Vivo Status of Exogenously Applied Auxins:
A HPLC-Based Method to Characterize the Intracellularly Localized Auxin Transporters.”
Plant Chemical Genomics, edited by Glenn Hicks and Stéphanie Robert, vol.
1056, Springer, 2014, pp. 255–64, doi:10.1007/978-1-62703-592-7_23.'
short: S. Simon, P. Skůpa, P. Dobrev, J. Petrášek, E. Zažímalová, J. Friml, in:,
G. Hicks, S. Robert (Eds.), Plant Chemical Genomics, Springer, 2014, pp. 255–264.
date_created: 2018-12-11T11:56:32Z
date_published: 2014-01-01T00:00:00Z
date_updated: 2025-07-10T11:52:16Z
day: '01'
department:
- _id: JiFr
doi: 10.1007/978-1-62703-592-7_23
editor:
- first_name: Glenn
full_name: Hicks, Glenn
last_name: Hicks
- first_name: Stéphanie
full_name: Robert, Stéphanie
last_name: Robert
fulldoi: https://doi.org/10.1007/978-1-62703-592-7_23
intvolume: ' 1056'
language:
- iso: eng
month: '01'
oa_version: None
page: 255 - 264
publication: Plant Chemical Genomics
publication_identifier:
issn:
- 1064-3745
publication_status: published
publisher: Springer
publist_id: '4704'
quality_controlled: '1'
scopus_import: '1'
series_title: Methods in Molecular Biology
status: public
title: 'Analyzing the in vivo status of exogenously applied auxins: A HPLC-based method
to characterize the intracellularly localized auxin transporters'
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1056
year: '2014'
...
---
_id: '6178'
abstract:
- lang: eng
text: Mechanically coupled cells can generate forces driving cell and tissue morphogenesis
during development. Visualization and measuring of these forces is of major importance
to better understand the complexity of the biomechanic processes that shape cells
and tissues. Here, we describe how UV laser ablation can be utilized to quantitatively
assess mechanical tension in different tissues of the developing zebrafish and
in cultures of primary germ layer progenitor cells ex vivo.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Michael
full_name: Smutny, Michael
id: 3FE6E4E8-F248-11E8-B48F-1D18A9856A87
last_name: Smutny
orcid: 0000-0002-5920-9090
- first_name: Martin
full_name: Behrndt, Martin
id: 3ECECA3A-F248-11E8-B48F-1D18A9856A87
last_name: Behrndt
- first_name: Pedro
full_name: Campinho, Pedro
id: 3AFBBC42-F248-11E8-B48F-1D18A9856A87
last_name: Campinho
orcid: 0000-0002-8526-5416
- first_name: Verena
full_name: Ruprecht, Verena
id: 4D71A03A-F248-11E8-B48F-1D18A9856A87
last_name: Ruprecht
orcid: 0000-0003-4088-8633
- first_name: Carl-Philipp J
full_name: Heisenberg, Carl-Philipp J
id: 39427864-F248-11E8-B48F-1D18A9856A87
last_name: Heisenberg
orcid: 0000-0002-0912-4566
citation:
ama: 'Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. UV laser ablation
to measure cell and tissue-generated forces in the zebrafish embryo in vivo and
ex vivo. In: Nelson C, ed. Tissue Morphogenesis. Vol 1189. MIMB. New York:
Springer; 2014:219-235. doi:10.1007/978-1-4939-1164-6_15'
apa: 'Smutny, M., Behrndt, M., Campinho, P., Ruprecht, V., & Heisenberg, C.-P.
J. (2014). UV laser ablation to measure cell and tissue-generated forces in the
zebrafish embryo in vivo and ex vivo. In C. Nelson (Ed.), Tissue Morphogenesis
(Vol. 1189, pp. 219–235). New York: Springer. https://doi.org/10.1007/978-1-4939-1164-6_15'
chicago: 'Smutny, Michael, Martin Behrndt, Pedro Campinho, Verena Ruprecht, and
Carl-Philipp J Heisenberg. “UV Laser Ablation to Measure Cell and Tissue-Generated
Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” In Tissue Morphogenesis,
edited by Celeste Nelson, 1189:219–35. MIMB. New York: Springer, 2014. https://doi.org/10.1007/978-1-4939-1164-6_15.'
ieee: 'M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, and C.-P. J. Heisenberg,
“UV laser ablation to measure cell and tissue-generated forces in the zebrafish
embryo in vivo and ex vivo,” in Tissue Morphogenesis, vol. 1189, C. Nelson,
Ed. New York: Springer, 2014, pp. 219–235.'
ista: 'Smutny M, Behrndt M, Campinho P, Ruprecht V, Heisenberg C-PJ. 2014.UV laser
ablation to measure cell and tissue-generated forces in the zebrafish embryo in
vivo and ex vivo. In: Tissue Morphogenesis. Methods in Molecular Biology, vol.
1189, 219–235.'
mla: Smutny, Michael, et al. “UV Laser Ablation to Measure Cell and Tissue-Generated
Forces in the Zebrafish Embryo in Vivo and Ex Vivo.” Tissue Morphogenesis,
edited by Celeste Nelson, vol. 1189, Springer, 2014, pp. 219–35, doi:10.1007/978-1-4939-1164-6_15.
short: M. Smutny, M. Behrndt, P. Campinho, V. Ruprecht, C.-P.J. Heisenberg, in:,
C. Nelson (Ed.), Tissue Morphogenesis, Springer, New York, 2014, pp. 219–235.
corr_author: '1'
date_created: 2019-03-26T08:55:59Z
date_published: 2014-08-22T00:00:00Z
date_updated: 2026-04-16T10:31:19Z
day: '22'
department:
- _id: CaHe
doi: 10.1007/978-1-4939-1164-6_15
editor:
- first_name: Celeste
full_name: Nelson, Celeste
last_name: Nelson
external_id:
pmid:
- '25245697'
fulldoi: https://doi.org/10.1007/978-1-4939-1164-6_15
intvolume: ' 1189'
language:
- iso: eng
month: '08'
oa_version: None
page: 219-235
place: New York
pmid: 1
publication: Tissue Morphogenesis
publication_identifier:
eisbn:
- '9781493911646'
eissn:
- 1940-6029
isbn:
- '9781493911639'
issn:
- 1064-3745
publication_status: published
publisher: Springer
quality_controlled: '1'
series_title: MIMB
status: public
title: UV laser ablation to measure cell and tissue-generated forces in the zebrafish
embryo in vivo and ex vivo
type: book_chapter
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
volume: 1189
year: '2014'
...
---
_id: '10900'
abstract:
- lang: eng
text: Leukocyte migration through the interstitial space is crucial for the maintenance
of tolerance and immunity. The main cues for leukocyte trafficking are chemokines
thought to directionally guide these cells towards their targets. However, model
systems that facilitate quantification of chemokine-guided leukocyte migration
in vivo are uncommon. Here we describe an ex vivo crawl-in assay using explanted
mouse ears that allows the visualization of chemokine-dependent dendritic cell
(DC) motility in the dermal interstitium in real time. We present methods for
the preparation of mouse ear sheets and their use in multidimensional confocal
imaging experiments to monitor and analyze the directional migration of fluorescently
labelled DCs through the dermis and into afferent lymphatic vessels. The assay
provides a more physiological approach to study leukocyte migration than in vitro
three-dimensional (3D) or 2-dimensional (2D) migration assays such as collagen
gels and transwell assays.
acknowledgement: We would like to thank Alexander Eichner and Ingrid de Vries for
discussion and critical reading of the manuscript, and Mary Frank for assistance
with the recording of videos and images in Fig. 1. M.S. is supported through funding
from the German Research Foundation (DFG). M.W. acknowledges the Alexander von Humboldt
Foundation for funding.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Michele
full_name: Weber, Michele
id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
last_name: Weber
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: 'Weber M, Sixt MK. Live Cell Imaging of Chemotactic Dendritic Cell Migration
in Explanted Mouse Ear Preparations. In: Cardona A, Ubogu E, eds. Chemokines.
Vol 1013. MIMB. Totowa, NJ: Humana Press; 2013:215-226. doi:10.1007/978-1-62703-426-5_14'
apa: 'Weber, M., & Sixt, M. K. (2013). Live Cell Imaging of Chemotactic Dendritic
Cell Migration in Explanted Mouse Ear Preparations. In A. Cardona & E. Ubogu
(Eds.), Chemokines (Vol. 1013, pp. 215–226). Totowa, NJ: Humana Press.
https://doi.org/10.1007/978-1-62703-426-5_14'
chicago: 'Weber, Michele, and Michael K Sixt. “Live Cell Imaging of Chemotactic
Dendritic Cell Migration in Explanted Mouse Ear Preparations.” In Chemokines,
edited by Astrid Cardona and Eroboghene Ubogu, 1013:215–26. MIMB. Totowa, NJ:
Humana Press, 2013. https://doi.org/10.1007/978-1-62703-426-5_14.'
ieee: 'M. Weber and M. K. Sixt, “Live Cell Imaging of Chemotactic Dendritic Cell
Migration in Explanted Mouse Ear Preparations,” in Chemokines, vol. 1013,
A. Cardona and E. Ubogu, Eds. Totowa, NJ: Humana Press, 2013, pp. 215–226.'
ista: 'Weber M, Sixt MK. 2013.Live Cell Imaging of Chemotactic Dendritic Cell Migration
in Explanted Mouse Ear Preparations. In: Chemokines. Methods in Molecular Biology,
vol. 1013, 215–226.'
mla: Weber, Michele, and Michael K. Sixt. “Live Cell Imaging of Chemotactic Dendritic
Cell Migration in Explanted Mouse Ear Preparations.” Chemokines, edited
by Astrid Cardona and Eroboghene Ubogu, vol. 1013, Humana Press, 2013, pp. 215–26,
doi:10.1007/978-1-62703-426-5_14.
short: M. Weber, M.K. Sixt, in:, A. Cardona, E. Ubogu (Eds.), Chemokines, Humana
Press, Totowa, NJ, 2013, pp. 215–226.
corr_author: '1'
date_created: 2022-03-21T07:47:41Z
date_published: 2013-04-03T00:00:00Z
date_updated: 2024-10-09T21:02:37Z
day: '03'
department:
- _id: MiSi
doi: 10.1007/978-1-62703-426-5_14
editor:
- first_name: Astrid
full_name: Cardona, Astrid
last_name: Cardona
- first_name: Eroboghene
full_name: Ubogu, Eroboghene
last_name: Ubogu
external_id:
pmid:
- '23625502'
fulldoi: https://doi.org/10.1007/978-1-62703-426-5_14
intvolume: ' 1013'
language:
- iso: eng
month: '04'
oa_version: None
page: 215-226
place: Totowa, NJ
pmid: 1
publication: Chemokines
publication_identifier:
eisbn:
- '9781627034265'
eissn:
- 1940-6029
isbn:
- '9781627034258'
issn:
- 1064-3745
publication_status: published
publisher: Humana Press
quality_controlled: '1'
scopus_import: '1'
series_title: MIMB
status: public
title: Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse
Ear Preparations
type: book_chapter
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 1013
year: '2013'
...